The invention relates to a culture medium and a method for inducing human amniotic epithelial stem cells to be differentiated into melanocytes. Endothelin 3, dexamethasone, stem cell factors, basic fibroblast growth factors, insulin-iron-selenium transducin and fetal bovine serum are added into an alpha-MEM basic culture medium. The method comprises the following steps: preparing human amniotic membranes into a human amniotic epithelial stem cell suspension solution; adding cells into the alpha-MEM basic culture medium added with the endothelin 3, the dexamethasone, the stem cell factors, thebasic fibroblast growth factors, the insulin-iron-selenium transducin and the fetal bovine serum; culturing in an incubator with the temperature of 37 DEG C and 5 percent of CO2 to obtain an amnioticepithelial stem cell adherent single layer; gradually inducing to be differentiated into the melanocytes of melanocyte marker gene tyrosinase-associated proteins 1 (trp 1) and 2 (trp 2), a microphthalmia-associated transcription factor (mitf) and positive tyrosinase (dtc). The culture medium and the method, provided by the invention, have the characteristics of abundant cell sources, low immunogenicity, no ethical limitation and simple induction method.