Culture medium and method for inducing human amniotic epithelial stem cells to be differentiated into melanocytes

Abstract

The invention relates to a culture medium and a method for inducing human amniotic epithelial stem cells to be differentiated into melanocytes. Endothelin 3, dexamethasone, stem cell factors, basic fibroblast growth factors, insulin-iron-selenium transducin and fetal bovine serum are added into an alpha-MEM basic culture medium. The method comprises the following steps: preparing human amniotic membranes into a human amniotic epithelial stem cell suspension solution; adding cells into the alpha-MEM basic culture medium added with the endothelin 3, the dexamethasone, the stem cell factors, thebasic fibroblast growth factors, the insulin-iron-selenium transducin and the fetal bovine serum; culturing in an incubator with the temperature of 37 DEG C and 5 percent of CO2 to obtain an amnioticepithelial stem cell adherent single layer; gradually inducing to be differentiated into the melanocytes of melanocyte marker gene tyrosinase-associated proteins 1 (trp 1) and 2 (trp 2), a microphthalmia-associated transcription factor (mitf) and positive tyrosinase (dtc). The culture medium and the method, provided by the invention, have the characteristics of abundant cell sources, low immunogenicity, no ethical limitation and simple induction method.

Description

technical field [0001] The invention belongs to the field of biotechnology, and relates to a culture medium and a method thereof for differentiation of epithelial stem cells into melanocytes, and specifically relates to a culture medium for inducing differentiation of human amniotic epithelial stem cells into melanocytes and a method thereof. Background technique [0002] Skin pigmentation plays a vital role in human health and skin aesthetics, especially in exposed areas such as the face. Since the pigment synthesized by melanocytes can protect the body from environmental attacks and the invasion of various dangerous cells, the lack of skin pigment can not only lead to immune diseases such as vitiligo, but also affect the mental health of patients. [0003] Vitiligo is a common acquired localized or generalized skin depigmentation disease. Due to the loss of melanocyte function in the skin, it can occur in various parts of the body, and it is common in the back of the fing...

AI Technical Summary

Problems solved by technology

[0005] The present invention aims at problems such as difficulty in source of autologous melanocytes and restriction of immunogenicity of allogeneic melanocytes in the existing methods for treating vitiligo, and provides a kind of human amniotic epithelial stem cells that have a wide range of allogeneic sources and no or low immunogenicity. Cell induction differentiation medium and method thereof

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