29 results about "Epithelial Stem Cell" patented technology

The invention discloses an amniotic epithelial stem cell screening method and device based on microscopic image feature extraction, relates to the technical field of microscopic image analysis and cell screening, and is used for solving the problem that group topology and internal activity in amniotic epithelial stem cell lossless screening are difficult to collaboratively quantify. The method comprises the following steps: firstly, performing enhancement processing on a phase difference microscopic image, and extracting a kernel centroid and a global gradient energy diagram; then, constructing a Voronoi jurisdiction, and coupling a cytoplasm texture entropy and a gray scale attenuation gradient to generate a cytoplasm compactness factor; meanwhile, calculating a cell gap topology consistency index based on mapping of the Voronoi boundary and the gradient map; and finally, weighting and correcting the compactness factor by using a topological index to obtain a dryness maintenance confidence coefficient, and outputting a target coordinate. Through dual verification of geometric topology and physical gradient, combined constraint screening of internal and external characteristics of cells is realized.

Methods and systems are described for a high-level approach to organ generation by self-assembly using bioprinting, overcoming longstanding challenges in replicating the intricate architecture and function of complex organs. In some embodiments, these methods are used to produce lung tissues and microtissues, demonstrating how mini-lung constructs can be produced on a small scale through specialized processes and media. In such embodiments, methods and systems for generating bioprinted lung tissues and microtissues are provided, as are tissues made by such methods. The tissues find use in implantation, drug discovery, personalized medicine, and other applications. Methods for the large-scale manufacturing of lung epithelial stem cells are also provided.

The application discloses an amnion epithelial stem cell screening method and device based on microscopic image feature extraction, relates to the technical field of microscopic image analysis and cell screening, and is used for solving the problem that the population topology and internal activity are difficult to be quantified in the lossless screening of amnion epithelial stem cells. Firstly, phase contrast microscopic images are subjected to enhancement processing, and nucleolus centers and global gradient energy maps are extracted; subsequently, Voronoi districts are constructed, and cytoplasm texture entropy and gray attenuation gradient are coupled to generate a cytoplasm compactness factor; meanwhile, a cell gap topology consistency index is calculated based on the mapping of the Voronoi boundary and the gradient map; finally, the compactness factor is weighted and corrected by using the topology index to obtain stemness maintenance confidence, and target coordinates are output. Through the dual verification of geometric topology and physical gradient, the application realizes the joint constraint screening of the features inside and outside the cells.

Methods and compositions for treating a corneal wound and / or increasing a population of limbal epithelial stem cells (LESCs) and / or early transit amplifying cells (eTAs) in the limbal epithelium of a subject in need thereof. The methods can include administering a therapeutic agent to the subject that: i) results in an increase in concentration of IFITM1 (Interferon Induced transmembrane Protein 1) in the limbal epithelium of the subject; and / or ii) results in a decrease in concentration of OVOL1 (Ovo Like Zinc Finger 1) in the limbal epithelium of the subject. Also provided are adeno-associated viruses (AAVs). The AAVs comprise a heterologous nucleic acid sequence encoding IFITM1 or a variant thereof, operably linked to a regulatory sequence.

An artificial hair follicle germ 10 has a three-dimensional structure in which a first block 11 including hair follicle epithelial stem cells, a second block 12 including hair papilla cells, and a third block 13 including dermal-derived mesenchymal cells (DDMCs) are stacked in this order. A method for artificially preparing a hair follicle germ 10 includes three-dimensionally culturing a first block 11 including hair follicle epithelial stem cells, a second block 12 including hair papilla cells, and a third block 13 including dermal-derived mesenchymal cells (DDMCs), these blocks being stacked in this order.

To provide an external composition that stably contains minoxidil in high concentration, and exhibits better hair growth effect than that of existing treatment agents.SOLUTION: An external composition contains 2 w / v% or more of minoxidil, the minoxidil being fine particles with an average particle size of 50 nm-200 nm. The external composition preferably further contains cellulosic polymers.SELECTED DRAWING: Figure 3

The present invention relates to functionally improved epithelial stem cells. The epithelial stem cells with improved functionality by a method of the present invention are prepared without genetic modification, thus being less likely to cause side effects due to DNA delivery / modification, are derived from the amniotic membrane in which no tissue-compatible antigen (factor) is expressed, thus having no rejection, and exhibit improved cell migration and would healing effects due to the improved functionality. Therefore, the epithelial stem cells can be used for the regeneration of various tissues, including the cornea, amniotic membrane, skin, and the like, and particularly can be used for the prevention or treatment of premature rupture of the amniotic membrane.

To provide a defined culture medium for isolating stratified epithelial stem cells and stably maintaining the epigenetics of the stratified epithelial stem cells during multiple passages, and to provide clonal stratified epithelial stem cells isolated using the defined culture medium.SOLUTION: And a defined culture medium comprising each of ROCK (Rho kinase) inhibitors, mitogenic growth factors, insulin or IGF, TrkA inhibitors, and Oct4 activators, and comprising at least one of VEGF inhibitors, tyrosine kinase inhibitors, and / or FGF10 or FGF10 agonists, and optionally further comprising TGF β signaling pathway inhibitors and / or bone morphogenetic proteins (BMP) antagonists, wherein the defined culture medium supports epigenetically stable growth and proliferation of stem cells of stratified epithelial tissue origin in culture.SELECTED DRAWING: Figure 1

The application provides a method for constructing a tissue-engineered cornea based on human amniotic epithelial stem cells and application thereof, and belongs to the technical field of biotechnology. The method comprises the following steps: 1) culturing human amniotic epithelial stem cells in a corneal epithelial cell induction and differentiation culture medium to obtain human corneal epithelial cell-like cells; culturing the human amniotic epithelial stem cells in a corneal stromal cell induction and differentiation culture medium to obtain corneal stromal cells; 2) inoculating the human corneal epithelial cell-like cells and the corneal stromal cells obtained in step 1) on the surface and inside of a decellularized corneal stromal scaffold respectively, and co-culturing for 5 days to construct a tissue-engineered cornea. The application successfully constructs a tissue-engineered cornea by inoculating two kinds of corneal cells derived from human amniotic epithelial stem cells on a human decellularized corneal stromal at the same time for the first time. In addition, the obtained corneal cells can be inoculated on various types of biomaterials such as hydrogels, thereby providing a wider application possibility for developing various types of artificial cornea products.

This invention discloses the application of genes derived from the rumen abdominal sac tissue of ruminants in promoting the differentiation of rumen epithelial stem cells, belonging to the field of genetic engineering technology. This invention is the first to discover genes with unknown functions. ABCC1、NPC1L1 , MRPL24 or GPR15LG The proteins encoded by these substances promote the differentiation of rumen epithelial stem cells, and this can be achieved by regulating gene expression or utilizing their encoded proteins to regulate the proliferation and differentiation of rumen epithelial stem cells. The research results of this invention overcome the limitations of traditional breeding methods that rely on phenotypic selection, by screening… ABCC1、NPC1L1 , MRPL24 or GPR15LG This study provides a reference for individuals with high gene expression and for the targeted breeding of breeds with more efficient rumen function using biological methods, and provides a core target for the breeding of new ruminant breeds that are high-yielding, efficient, and stress-resistant.

This invention relates to the application of human amniotic epithelial stem cells (hAESCs) in retinal regeneration and repair, belonging to the field of biomedical technology. It aims to address the technical problems of off-target effects, low efficiency, and safety risks associated with existing methods that directly reprogram Müller glial cells (MGs) via viruses. This invention transplants hAESCs into the degenerated subretinal space. hAESCs, through secreting active substances such as matrix metalloproteinases (MMPs), degrade excessively deposited extracellular matrix, improve the pathological microenvironment inhibiting regeneration, and thus promote the reprogramming of endogenous MGs into functional retinal neurons, thereby achieving retinal structural repair and visual function restoration. This invention utilizes highly safe hAESCs and, by remodeling the microenvironment, significantly improves the efficiency and specificity of MG differentiation into functional neurons, providing a new strategy for the treatment of retinal degenerative diseases.

Epithelial stem cells (15), such as hair follicle epithelial stem cells, are cultured on a scaffold (13) comprising a molecule containing an extracellular cadherin (cadherin EC) domain. The epithelial stem cells 15 are then separated from the scaffold 13. The epithelial stem cells 15 are prepared through the abovementioned process. Thereafter, a hair follicle primordium10 may be produced by three-dimensionally culturing the epithelial stem cells 15 and mesenchymal cells 16 in a state in which a layer of the epithelial stem cells 15 and a layer of the mesenchymal cells 16 overlap. The mesenchymal cell 16 layer may include a layer of hair papilla cells and a layer of cells constituted of dermal pluripotent cells (DDMCs) or dermal sheath cells (DS cells). The epithelial stem cell 15 layer may overlap the hair papilla cell-layer side of the mesenchymal cell 16 layer.

The invention relates to cell biology and regenerative medicine, specifically to a culture method and application for porcine limbal epithelial stem cells. The method involves pretreatment of cells, coating of culture dishes, centrifugation, culturing, passaging, and identification by immunofluorescence staining. The cultured cells are applicable in repairing corneal diseases, including limbal stem cell deficiency. During repair, the cells can be combined with biological materials to form a stem cell engineering carrier for implantation at the injury site. The separation and culture method is simple, yields a large number of cells, and is suitable for use with porcine eyeballs. Limbal epithelial stem cells cultured in vitro form distinct cell clusters, referred to as stem cell islands. The obtained cells exhibit phenotypes including BMI1+, ΔNp63α+, CK14+, and ABCG2+. Based on experimental and clinical research, this method provides a promising approach for cell transplantation in ocular surface reconstruction.

Provided herein include methods, compositions, isolated cells, cell models, and kits suitable for use in profiling various cell types in amnion including amnion epithelial cells, amnion mesenchymal cells, fibroblasts, macrophages, as well as amnion stem cells including amnion epithelial stem cells and amnion mesenchymal stem cells. The present disclosure also discloses novel markers for various cell types in human amnion, including marker genes, transcription factors and signaling molecules associated with various stages of amniotic development. The methods, cell models, and gene signatures disclosed herein can be used for a variety of research, diagnostic, or therapeutic applications.

The invention relates to differentiation methods for progenitor cells, e.g. mammalian epithelial stem cells, differentiation media for use in said methods, organoids and cells obtainable by said methods and uses, including therapeutic uses, thereof.

The present application relates to the field of cell detection and screening, and particularly relates to a method and system for detection and screening of amniotic epithelial stem cells. The method comprises: generating a structured sample task sheet based on multi-source information; performing tissue digestion processing based on the task sheet to obtain a candidate cell group; then performing phenotype detection, molecular detection and quality detection to generate corresponding data packets and label data packets; based on the quality label data packets, performing gating, batch normalization, abnormality rejection, fusion scoring and consistency determination processing through a screening engine to generate a screening result packet; and finally generating a disposal work order and a traceability report based on the screening result packet. The present application realizes standardized and auditable automatic processing of the whole process of amniotic epithelial stem cells from receiving, detection to sorting and disposal, greatly improving the detection efficiency, result consistency and traceability.

The application belongs to the technical field of biological medicine, and particularly relates to a thymus tissue dissociation reagent, a kit and application thereof in obtaining thymus epithelial cells. The thymus tissue dissociation reagent at least comprises collagenase D, dispersinase II, deoxyribonuclease I, dissociation enzyme and a dissolving reagent; and collagenase II and / or collagenase IV and / or trypsin. The cell survival rate of the thymus tissue dissociation reagent after dissociating the thymus tissue is maintained at a high level of 95.7% to 99.6%, which is significantly higher than that of the prior art; and after sorting, the number of thymus epithelial cells enriched and obtained from each gram of thymus tissue is 1.38e 5 ~1.06e 6 , and the success rate of enriching and obtaining thymus epithelial cells is as high as 95%, which is significantly higher than that of the prior art; and the proportion of epithelial stem cells in the thymus epithelial cells is 65.7%, which is significantly higher than that of the prior art.

An atlas of intestinal epithelial cells, intestinal epithelial stem cells and intestinal immune cells identifies new cell populations, markers, networks, and responses to stimuli. Intestinal epithelial cell sub-types are also found in the trachea. Accordingly, disclosed are methods of modulating epithelial cell differentiation, maintenance and / or function, related methods for the treatment of disease, including IBD and asthma. Also disclosed are methods and kits for identifying cell types, their differentiation, homeostasis and activation.

Methods of isolating normal stem cells in culture derived from embryonic epithelial tissue and cancer stem cells derived from epithelial cancers and culture medium systems useful for epigenetically stable growth SOLUTION: A method for isolating stem cells from epithelial tissue, preferably columnar epithelial tissue, comprising: (I) culturing dissociated epithelial cells from a columnar epithelial tissue sample to form a stem cell colony; (ii) isolating single stem cells from the cell colony; and (iii) individually culturing the isolated single stem cells from step (ii) to form a culture of purified stem cell clones, wherein: Wherein each of the stem cell clones represents a clonal expansion of epithelial stem cells present in the columnar epithelial tissue sample, thereby isolating epithelial stem cells.SELECTED DRAWING: None

The invention discloses application of human amniotic epithelial stem cells in preparation of drugs for treating depression diseases. The invention provides application of human amniotic epithelial stem cells and biological materials thereof in preparation of drugs for treating depression diseases. According to the human amniotic epithelial stem cells, amniotic membranes can be separated from the inner surfaces of discarded in-vitro placenta tissues through a mechanical means, then the amniotic membranes are cleaned, and the human amniotic epithelial stem cells are harvested through pancreatin digestion, centrifugation and resuspension in sequence. The adopted human amniotic epithelial stem cells are ideal schemes for treating depression and pharmacy, and the hAESCs have the advantages that the source is easy to obtain, the hAESCs are easy to obtain and have no ethical problems; by inhibiting excessive activation of glial cells, inhibiting inflammatory response related to depression and improving a neuron microenvironment, apoptosis of neurons is reduced, and depression-like behaviors of mice are effectively improved.

The invention discloses application of human amniotic epithelial stem cells in preparation of drugs for treating depression diseases. The human amniotic epithelial stem cells are harvested after being subjected to pancreatin digestion, centrifugation and resuspension in sequence after being cleaned by mechanically separating an amnion on the inner surface of a discarded in-vitro placenta tissue; and a human amniotic epithelial stem cell regimen for the treatment of depression; the cell is wide in source, free of ethical dispute, low in immunogenicity and free of tumorigenicity, and a novel safe and effective way is provided for depression treatment. The cell preparation can be administered through intravenous injection, intrathecal injection or nasal delivery, and the action mechanism of the cell preparation is that the cell preparation can inhibit excessive activation of glial cells and relieve depression-related neuroinflammatory response, so that the neuron microenvironment is improved. The traditional Chinese medicine composition can effectively promote hippocampal neurogenesis (DCX positive cell increase and the like), regulate the activity of 5-hydroxytryptamine neurons and glutamic acid neurons to restore the steady state of a neural network, and remarkably improve the depression-like behavior of a model mouse.

The application discloses a lacrimal passage epithelial organoid and a construction method and application thereof, and the construction method comprises the following steps: S1, preparation of a three-dimensional biomimetic support; S2, acquisition of lacrimal passage epithelial stem cells; and S3, construction and culture of the organoid. The PCL / GEL nanofiber support constructed by the electrospinning technology is combined with Matrigel coating, the extracellular matrix (ECM) microenvironment of the in-vivo lacrimal passage mucosa is successfully simulated, and the technical difficulties that stem cells are prone to aging and differentiation direction disorder in traditional two-dimensional culture are fundamentally solved. This breakthrough result promotes the lacrimal passage tissue engineering from simple 'cell adhesion growth' to a new height of'structure-function synchronous reconstruction', and provides a double technical guarantee of seed cells and tissue engineering supports for solving the clinical core pain point of postoperative scar adhesion and re-occlusion of the lacrimal passage obstruction disease.

The invention relates to differentiation methods for progenitor cells, e.g. mammalian epithelial stem cells, differentiation media for use in said methods, organoids and cells obtainable by said methods and uses, including therapeutic uses, thereof.

This invention provides a method for culturing bronchial epithelial stem / progenitor cells. Specifically, this invention provides a culture medium for culturing bronchial epithelial stem / progenitor cells, comprising a basal culture medium and additives; wherein the basal culture medium is selected from the group consisting of DMEM, Ham's F-12, DMEM / F-12, or combinations thereof, and the additives include: fetal bovine serum, glucose, insulin, epidermal growth factor, hydrocortisone, Y-27632, SB431542, Noggin, and R-spondin. 1. Fibronectin and E-Cadherin are provided, along with a method for culturing bronchial epithelial stem / progenitor cells. The bronchial epithelial stem / progenitor cells amplified using the method of this invention have a high cloning rate, can be isolated from trace amounts of tissue, and can be passaged more than 50 times. The cells maintain normal stemness and differentiation potential, and can be cultured to obtain a sufficient number of clinically infused bronchial epithelial stem / progenitor cells for autologous infusion. Clinically, this has achieved the repair of small airway damage in the lungs and significantly improved lung function.

The application belongs to the technical field of exosome application, and discloses an exosome composition for preventing hair loss and fixing hair. The exosome composition is obtained by adding plant exosomes in animal stem cell culture, and it is found that the plant exosomes can significantly promote the secretion of animal stem cell exosomes. On this basis, a scheme for constructing the exosome composition for preventing hair loss and fixing hair is constructed. The exosome composition is obtained by mixing fat stem cell exosomes secreted by fat stem cells treated by curcuma exosomes, urine source epithelial stem cell exosomes secreted by urine source epithelial stem cells treated by olea europaea leaf exosomes and biota leaf exosomes according to weight proportions of 1-2:2-5:2-3. The exosome composition can significantly promote hair papilla cell proliferation, has a significant effect on preventing hair loss and fixing hair, has a large number of plant exosomes, can be regenerated, can significantly reduce the use amount of animal stem cell exosomes, and has a better effect after combination, and has a wide application prospect.

Provided herein include methods, compositions, isolated cells, cell models, and kits suitable for use in profiling various cell types in amnion including amnion epithelial cells, amnion mesenchymal cells, fibroblasts, macrophages, as well as amnion stem cells including amnion epithelial stem cells and amnion mesenchymal stem cells. The present disclosure also discloses novel markers for various cell types in human amnion, including marker genes, transcription factors and signaling molecules associated with various stages of amniotic development. The methods, cell models, and gene signatures disclosed herein can be used for a variety of research, diagnostic, or therapeutic applications.