55 results about "Isorhamnetin" patented technology

The invention discloses an application of isorhamnetin in preparation of an anti-myocardial fibrosis medicine, and belongs to the technical field of medicines. The invention proposes that the isorhamnetin can effectively reduce myocardial tissue collagen deposition for the first time, and the medical application of the isorhamnetin is expanded. The isorhamnetin is used as the anti-myocardial fibrosis medicine, can effectively relieve the increase of mRNA expression levels of alpha-SMA, COL-1, COL-3, p53, PAI-1, IL-1beta and IL-6, and has the function of retarding myocardial fibrosis. The isorhamnetin is used as the active component of the anti-myocardial fibrosis medicine, the safety is high, the side effect is small, a novel anti-myocardial fibrosis treatment strategy is provided, and the application blank of the isorhamnetin in the field is filled.

To provide a novel erythropoietin production promoter.SOLUTION: An erythropoietin production promoter contains isorhamnetin and / or an isorhamnetin glycoside as an active ingredient.SELECTED DRAWING: Figure 1

The present application belongs to the technical field of traditional Chinese medicine detection, and particularly relates to a method for determining the content of multiple flavone components in Tibetan medicine Sanwei Huangjing Tangsan, which simultaneously determines the content of three flavone aglycone, i.e., quercetin, kaempferol and isorhamnetin in Sanwei Huangjing Tangsan by using high performance liquid chromatography (HPLC). The present application can accurately determine the content of main flavone effective components in Sanwei Huangjing Tangsan, has a good linear range, high accuracy, precision and repeatability, and is stable and feasible, and can be applied to the quality inspection of Sanwei Huangjing Tangsan.

The invention belongs to the field of pharmaceutical preparations, and particularly relates to a sulfhydrylated hyaluronic acid drug-loaded hydrogel preparation and a preparation method and application thereof, and the preparation method comprises the following steps: preparing isorhamnetin nanocrystalline ISO-NCs by adopting an anti-solvent precipitation method; the preparation method comprises the following steps: constructing thiolated hyaluronic acid HA-SH responded by GSH; and uniformly embedding the ISO-NCs into the HA-SH hydrogel, so as to prepare the sulfhydrylated hyaluronic acid drug-loaded hydrogel preparation ISO-NCs-coated Gel, and preparing the sulfhydrylated hyaluronic acid drug-loaded hydrogel preparation ISO-NCs-coated Gel. The drug-loaded hydrogel preparation constructed by the invention can be used for targeted therapy of colon cancer.

The invention discloses application of isorhamnetin in preparation of antiarrhythmic drugs, and belongs to the technical field of medicines. The isorhamnetin is proposed for the first time to effectively improve myocardial conduction rate increase, conduction discrete index increase and heart rate acceleration symptoms of tachyarrhythmia patients, and the medical application of the isorhamnetin is expanded. The isorhamnetin serving as the anti-arrhythmia drug has a very good inhibition effect on increase of cAMP / PKA signal transduction pathways, realizes reversal of occurrence of arrhythmia and has important guiding significance in treatment of arrhythmia. The isorhamnetin is used as the active ingredient of the anti-arrhythmia medicine, the safety is high, the side effect is small, a new way is provided for development of the anti-arrhythmia medicine, and the application blank of the isorhamnetin in the field is filled.

This invention relates to a method for creating low-cadmium-accumulation, high-yield rice. The invention utilizes CRISPR / Cas9 technology to render the function of isorhamnetin diglyceride synthase in rice deficient, exhibiting significant advantages under cadmium stress: the mutant shows significantly higher aboveground part length, aboveground dry weight, and root length than the wild type under cadmium stress; cadmium accumulation at both the whole plant and subcellular levels is significantly reduced; and the cadmium content in grains at grain maturity is significantly lower than that of the wild type, while yield is no different from the wild type. This mutant achieves the triple goals of "cadmium stress resistance, low cadmium accumulation, and high yield and quality," reducing the risk of cadmium pollution while ensuring planting benefits, providing a new solution for safe rice production in cadmium-polluted areas.

The invention discloses a composition with a whitening effect. The composition is prepared from the following components in parts by mass: 47 to 51 parts of grape seed oil, 47 to 51 parts of pomegranate seed oil, 0.5 to 0.8 part of 4-butylresorcinol, 0.2 to 0.6 part of VC-IP (Vitamin C-IP) and 0.1 to 10 parts of isorhamnetin-3-O-neohesperidin. The invention also discloses a preparation method of the composition. The invention also discloses an essence oil containing the composition. The invention also discloses a face cream containing the composition. The invention also discloses application of the composition in preparation of cosmetics for skin whitening, spot fading and skin yellowing reduction. The composition provided by the invention establishes a multi-target synergistic pathway: 1, a blackness removal pathway for reducing the activation of oxidative stress such as ultraviolet rays on melanocytes; and 2, a jaundice removal pathway: inhibiting lipid peroxidation and promoting lipofuscin removal.

The application provides application of isorhamnetin in preparation of a drug for preventing and treating male lower urinary tract symptoms / prostatofibrosis. The application belongs to the technical field of biological medicine, and proves that isorhamnetin can effectively improve the prostatofibrosis level of db / db mice and relieve lower urinary tract symptoms through animal experiments, bioinformatics and network pharmacology methods, and isorhamnetin can play a role in improving prostatofibrosis by targeting PPAR gamma.

The invention relates to the technical field of biological medicines, in particular to application of isorhamnetin in preparation of a medicine for preventing and treating fatty liver diseases related to metabolic dysfunction. The invention provides application of isorhamnetin or pharmaceutically acceptable salts, esters or derivatives thereof in preparation of drugs for preventing and treating fatty liver diseases related to metabolic dysfunction. The invention also provides application of isorhamnetin or pharmaceutically acceptable salts, esters or derivatives thereof in preparation of drugs for preventing and treating diseases caused by intestinal flora disorder. The isorhamnetin can recover the diversity and richness of intestinal flora of mice with fatty liver diseases related to metabolic dysfunction and recover the balance of the intestinal flora, and the high-dose (50 mg / kg.d) donor flora transplantation of the isorhamnetin can partially reproduce metabolism improvement. The fatty liver diseases related to obesity or metabolic dysfunction are relieved through an'intestinal flora-bile acid-lipid metabolism 'axis.

The present application provides a kind of pharmaceutical composition with lipid-lowering effect, including luteolin, isorhamnetin, 9-hydroxy-4,7-megastigme-3-ketone, lotus leaf base, 3-O-feruloyl quinic acid, kaempferol-3-O-acacia disaccharide-7-O-glucoside, kaempferol-3-O-beta-D-acacia glycoside and ginsenoside Rg1.The composition of example 1 has lipid-lowering efficacy verified by golden hamster hyperlipidemia model and AML12, HepG2 cell fatty degeneration model, the regulation effect of the composition of example 1 on the key target points of AMPK signal pathway and NF-κB signal pathway is verified by using molecular pharmacology experiment, and the mechanism of the composition of example 1 in treating hyperlipidemia by regulating energy metabolism and anti-inflammatory mechanism is described.

The invention discloses a construction method of a finger-print spectrum of a styptic capsule and application of the styptic capsule in quality detection, and belongs to the field of Chinese patent medicine detection. The method comprises the following steps: firstly, adding 80% methanol solution into contents of different batches of Xuezhenning capsules for extraction to prepare Xuezhenning capsule test solution; the method comprises the following steps: taking ginsenoside Rg1, notoginsenoside R2, ginsenoside Rd, caffeic acid, isorhamnetin, verbascoside, aesculetin, ferulic acid, rutin and quercetin as reference substances, and preparing a reference substance solution; respectively injecting the test solution and the reference solution into a high performance liquid chromatograph to obtain corresponding chromatograms; performing similarity analysis on the chromatogram of the test solution; and finally, according to the chromatogram of the test solution and the chromatogram of the reference substance solution, combining a total ion chromatogram obtained by the high-resolution mass spectrum and a mass spectrum result map of the chemical components to construct the fingerprint spectrum of the styptic Ning capsule. The method can be used for simultaneously detecting 10 components in the haemostatic Ning capsule, and can be used for accurately, clearly and objectively evaluating the quality of the haemostatic Ning capsule.

The invention is applicable to the technical field of biological medicine, and provides application of isorhamnetin-3-O-neohesperidin in preparation of a medicine for treating alopecia. Research finds that isorhamnetin-3-O-neohesperidin can promote hair growth and protect hair follicle stem cells, and as a natural extract, the isorhamnetin-3-O-neohesperidin can be used for preparing a medicine for treating alopecia. The traditional Chinese medicine composition has the advantages of being low in cost, few in side effect, good in patient compliance, easy to obtain and the like, and a new thought is provided for preparing the medicine for treating alopecia, especially the medicine for treating androgenetic alopecia.

The invention relates to the technical field of coreopsis tinctoria refined components and preparation and application, and provides a coreopsis tinctoria refined component and a preparation method and application thereof.The coreopsis tinctoria refined component comprises, by mass, 11 specific components of phenolic acids, flavonol, flavanol and flavonoid, and the 11 specific components comprise, by mass, 2 parts of gallic acid, 1 part of potassium sorbate, 1 part of potassium sorbate, 1 part of potassium sorbate and 1 part of potassium sorbate. The health-care food is prepared from the following raw materials in parts by weight: 3 parts of chlorogenic acid, 14 parts of quercetin, 18 parts of catechin, 5 parts of citric acid, 2-3 parts of procyanidine B, 3 parts of (+)-epicatechin, 1-31 parts of 31 parts of procyanidine C, 14 parts of rutin, 4 parts of camphanol-3-O-rutinoside and 2 parts of isorhamnetin-3-O-rutinoside. The snow chrysanthemum is Changbai Mountain snow chrysanthemum. Animal experiments show that the refined component can significantly reduce the content of total cholesterol and triglyceride in serum of a high-fat model rat, the specific component with blood fat regulating activity is prepared from Changbai Mountain snow chrysanthemum, and a new choice is provided for non-drug intervention of dyslipidemia.

The invention relates to a preparation method of a hippophae rhamnoides leaf isorhamnetin glycoside enriched solid beverage, and belongs to the field of functional plant beverage processing. The preparation method comprises the following steps: preparing sea buckthorn green tea and sea buckthorn green tea freeze-dried powder by taking sea buckthorn leaves as a main raw material; carrying out ethanol extraction and AB-8 type macroporous resin separation and purification to obtain an isorhamnetin glycoside substance enriched component; a Maillard composite system formed by whey protein isolate and maltodextrin is loaded with an isorhamnetin glycoside substance enrichment component, the isorhamnetin glycoside substance enrichment component is compounded with sea buckthorn green tea freeze-dried powder, freeze drying is performed, and the isorhamnetin glycoside enrichment solid beverage is obtained. The isorhamnetin glycoside enriched solid beverage disclosed by the invention has good solubility, and shows a remarkable immunological enhancement effect in a cyclophosphamide induced immunocompromised mouse model. The method has a wide application prospect in the aspects of high-value utilization of hippophae rhamnoides leaf resources and functional beverage development.

The invention discloses a gingko GbHCT1 gene as well as an expression protein and application thereof, and belongs to the field of plant molecular biology. The GbHCT1 gene is obtained by analyzing and screening based on data of a ginkgo transcriptome in the earlier stage of a research group, the nucleotide sequence of the GbHCT1 gene is as shown in SEQ ID NO.1, and the amino acid sequence of expression protein of the GbHCT1 gene is as shown in SEQ ID NO.2. A GbHCT1 overexpression vector is constructed and the nicotiana benthamiana is transformed, so that the content of ten flavonoid substances such as luteolin and isorhamnetin-3-O-glucoside in the transgenic GbHCT1 tobacco is remarkably increased. KEGG enrichment shows that differential metabolites are mainly enriched in a flavone synthesis pathway. The application reveals that the ginkgo GbHCT1 gene has dual effects on synthesis of flavonoid compounds by regulating carbon source distribution of a phenylpropane metabolic pathway, and a theoretical basis, a gene resource and a method are provided for secondary metabolism regulation and genetic improvement of ginkgo.

This invention discloses a method for determining the flavonoid aglycone content in acid-hydrolyzed sea buckthorn leaf extract, belonging to the field of detection technology. The invention involves acid-hydrolyzing sea buckthorn leaf extract to obtain a filtrate containing flavonoid aglycones, which is used as the test solution. High-performance liquid chromatography (HPLC) is used to detect the flavonoid aglycone content in both the test solution and the reference solution. The preparation method of the sea buckthorn leaf extract test solution rich in quercetin, kaempferol, and isorhamnetin includes: adding sea buckthorn leaf extract to methanol and hydrochloric acid solutions, mixing thoroughly, heating under reflux, cooling, shaking, and filtering to obtain the sea buckthorn leaf extract test solution rich in quercetin, kaempferol, and isorhamnetin. This invention simplifies the diverse chemical structures of flavonoid glycosides to a few flavonoid aglycones, effectively reducing dependence on expensive and scarce glycoside reference standards, simplifying chromatographic separation, and improving the stability and reproducibility of the method.

The invention provides a method for detecting HPLC (high performance liquid chromatography) characteristic chromatograms of senecio scandens medicinal materials, decoction pieces, standard decoction and formula granules, which comprises the following steps: pre-treating raw materials of a test sample to obtain liquid to be detected; the pretreatment mode comprises solvent dissolution and extraction; measuring the liquid to be measured by adopting HPLC (High Performance Liquid Chromatography) to obtain HPLC characteristic chromatograms of the senecio scandens medicinal material, decoction pieces, standard decoction and formula granules; the preparation method comprises the following steps: preparing a reference substance solution, namely dissolving neochlorogenic acid, protocatechuic aldehyde, chlorogenic acid, caffeic acid, cryptochlorogenic acid, p-coumaric acid, chlorogenic acid methyl ester, rutin, hyperoside, isoquercitrin, chlorogenic acid ethyl ester, isorhamnetin-3-O-galactoside, quercitrin, isochlorogenic acid B, isochlorogenic acid A, isochlorogenic acid C, quercetin and kaempferol by adopting a solvent; obtaining a reference substance solution of the reference substance; the HPLC conditions are as follows: a chromatographic column is a phenyl column; a mobile phase A is an acetonitrile solution, a mobile phase B is a 0.3% phosphoric acid aqueous solution, and gradient elution is carried out; according to the method, a high performance liquid chromatography method is adopted, and an acetonitrile-0. 3% phosphoric acid solution is selected as a mobile phase for gradient elution; the method comprises the following steps: taking neochlorogenic acid, protocatechuic aldehyde, chlorogenic acid, caffeic acid, cryptochlorogenic acid, p-coumaric acid, chlorogenic acid methyl ester, rutin, hyperoside, isoquercitrin, chlorogenic acid ethyl ester, isorhamnetin-3-O-galactoside, quercitrin, isochlorogenic acid B, isochlorogenic acid A, isochlorogenic acid C, quercetin and kaempferol as reference substances; the HPLC characteristic chromatogram method of the senecio scandens medicinal material, the decoction pieces, the standard decoction and the formula granules is established, and more scientific technical means are provided for controlling the medicinal quality of the senecio scandens medicinal material, the decoction pieces, the standard decoction and the formula granules.

The invention relates to the technical field of flavone separation and purification, in particular to a method for separating and purifying Yunnan sea buckthorn crude flavone. A high-speed counter-current chromatography and a preparative liquid chromatography are combined to separate and purify the Yunnan sea-buckthorn crude flavone, and the method comprises the following steps of: separating the Yunnan sea-buckthorn crude flavone by using normal hexane, ethyl acetate, methanol and water in a volume ratio of 1: 9: 1: 9 as well as ethyl acetate, n-butyl alcohol and water in a volume ratio of 4: 1: 5 as a solvent system and using the high-speed counter-current chromatography to separate the Yunnan sea-buckthorn crude flavone, the isorhamnetin-3-O-glucoside, the isoquercitrin and the rutin are obtained; and then, purifying the unseparated effluent through preparative liquid chromatography to obtain the kaempferol and the quercetin. According to the method, the high-speed counter-current chromatography and the preparative liquid chromatography are combined, so that the problems of long separation period and low purification efficiency of the traditional column chromatography are solved, and the aim of quickly separating and purifying various flavone compounds from the Yunnan sea-buckthorn crude flavone is fulfilled.

The invention provides a tobacco additive composition for preventing and treating sphagitis. The tobacco additive composition comprises isorhamnetin and naringenin. The tobacco additive has unique properties, when tobacco is ignited, isorhamnetin and naringenin are not changed through a combustion mode but are converted into a gaseous state, and the isorhamnetin and the naringenin enter the respiratory tract of the human body along with gas generated by tobacco combustion. Due to the characteristic, the traditional Chinese medicine composition can play a positive role in chronic pharyngitis and pharyngolaryngitis, effectively relieve symptoms, prevent diseases and assist the treatment process, does not cause any complications after being used for a long time, and greatly guarantees the health and safety of a user. From the perspective of use experience, the tobacco product is endowed with good taste by the additive, and the additive can be appreciated and accepted by the majority of smokers.

To provide use of isorhamnetin or pharmaceutically acceptable salts thereof in preparation of drugs for treating esophageal cancer in the technical field of biological medicines.SOLUTION: It has been found that isorhamnetin significantly inhibits malignant growth of metastatic esophageal squamous carcinoma cells and in-vivo ESCC metastatic tumors by inhibiting a fatty acid de novo synthesis pathway in tumor metabolism. Therefore, isorhamnetin can be used for developing an anti-ESCC metastatic cell growth medicine targeting an abnormally active fatty acid de novo anabolic enzyme.SELECTED DRAWING: Figure 1

The application belongs to the technical field of biological medicine, and provides a use of isorhamnetin-3-O neohesperidoside in preparation of a drug for treating alopecia, and finds that isorhamnetin-3-O neohesperidoside can promote hair growth and protect hair follicle stem cells, and as a natural extract, has many advantages such as low cost, few side effects, good patient compliance and easy to obtain, and provides a new idea for preparation of a drug for treating alopecia, in particular, a drug for treating androgenetic alopecia.

The invention discloses an EPE foamed sheet prepared from a mixed butane / HFO-1233zd composite foaming agent and a preparation method of the EPE foamed sheet, and belongs to the technical field of polyethylene foamed materials. The preparation method comprises the following steps: (1) adding low-density polyethylene, talcum powder, monoglyceride, tetra [beta-(3, 5-di-tert-butyl-4-hydroxyphenyl) propionic acid] pentaerythritol ester and isorhamnetin into an extrusion foaming machine to prepare a melt; and (2) respectively injecting mixed butane and HFO-1233zd into a third area of a machine barrel of an extrusion foaming machine, uniformly stirring the mixed butane and the HFO-1233zd with the melt, and then performing extrusion, foaming, natural cooling and cutting forming treatment to obtain the EPE foamed sheet. The residual amount of mixed butane in 48 hours of the EPE foamed sheet prepared by the invention is less than or equal to 25 ppm, and the EPE foamed sheet can be directly used subsequently.

The invention discloses an extraction reagent and an extraction method for functional components of natural plants. The method is applied to extraction of curcumin in turmeric, xanthophyll in marigold, linalool in lavender, rosmarinic acid in basil, ellagic acid in pomegranate, anthocyanin in lycium ruthenicum, isorhamnetin in sea-buckthorn, ginsenoside in ginseng and baicalin in scutellaria baicalensis. According to the method, the potential of DES in replacement of a traditional solvent can be visually displayed, and the method is suitable for courses of chemistry, environmental science or materials science and helps students to understand core concepts (such as recoverability and low-toxicity design) of green chemistry. Experiment cost is low, operation is safe, and the device is suitable for teaching laboratories.

The invention relates to the technical field of analysis method detection, and provides a detection method of a pharmaceutical composition. The detection method of the pharmaceutical composition comprises the following steps: extracting components of the pharmaceutical composition comprising tribulus terrestris, rhizoma polygonati and mirabilis himalaica, and preparing the extracted components into a test solution; dissolving a reference substance to prepare a reference substance solution; and respectively injecting the reference solution and the test solution into a liquid chromatograph, and detecting according to preset chromatographic conditions. Wherein the reference substance is prepared from 5-hydroxymethylfurfural, quercetin-3-O-gentiobioside and isorhamnetin-3-O-gentiobioside, and the reference substance is prepared from 2-hydroxymethylfurfural, quercetin-3-O-gentiobioside The detection method of the pharmaceutical composition can be used for quantitative analysis of multi-components by single marker, the detection efficiency is improved, and the detection cost is reduced.

The application discloses a construction method and content determination method of a fingerprint spectrum of Ginkgo biloba L., and belongs to the technical field of traditional Chinese medicine detection. A test sample solution is prepared through heating reflux extraction, data is collected through HPLC gradient elution, and a fingerprint spectrum containing 17 common peaks is constructed; meanwhile, the content of isorhamnetin-3-O-glucoside is determined through ultrasonic extraction combined with HPLC. The method has high extraction efficiency, good chromatographic peak separation degree, excellent precision and repeatability, can effectively identify Ginkgo biloba L. and its adulterants, accurately monitors the quality of the medicinal material, and provides a reliable basis for quality control of Ginkgo biloba L.

The present invention belongs to the field of traditional Chinese medicine detection, and specifically relates to the chromatographic identification of Typhae Pollen charcoal. Typhae Pollen charcoal in Fuke Duanhongyin capsules is identified using thin-layer chromatography. Identification bands are detected in both the test sample and the reference sample, isorhamnetin-3-O-neohesperidin solution, with good separation between the bands. In the negative control, no characteristic bands are detected in Fuke Duanhongyin capsules lacking Typhae Pollen charcoal, indicating no interference. The chromatographic identification method of Typhae Pollen charcoal has strong specificity and good durability, and is applicable to different thin-layer plates, at different temperatures and humidity levels. It can also be quickly and accurately identified even in high-humidity environments, and can be effectively used to identify Typhae Pollen charcoal in Fuke Duanhongyin capsules.

The present application provides a kind of determination wine Jumu and ginkgo leaf content in Yining Tongmai Jian Nao Granules, adopts liquid chromatography to determine the content of monorin, strychnos seed, kaempferol, isorhamnetin, including being analyzed by liquid chromatograph, with the mixed solution of monorin control product, strychnos seed control product being prepared as a mixed control product solution, with the mixed solution of kaempferol control product, isorhamnetin control product being prepared as another mixed control product solution, with the solution of Yining Tongmai Jian Nao Granules sample being prepared as test product solution, corresponding chromatographic conditions are set respectively, are precisely sucked respectively, then are injected into liquid chromatograph respectively, and content is determined.The quality of Yining Tongmai Jian Nao Granules is evaluated by the detection of the present application by liquid chromatography, which can better reflect the internal quality of the compound granules and better control the quality of Yining Tongmai Jian Nao Granules.

This invention discloses a method for constructing a fingerprint spectrum of a throat-clearing hard candy and a method for detecting its component content, belonging to the field of component detection technology. In this invention, the throat-clearing hard candy sample solution is analyzed by high-performance liquid chromatography (HPLC) to obtain a fingerprint spectrum of the candy; and the content information of the components is obtained through the external standard method, realizing qualitative and quantitative analysis of the components of the throat-clearing hard candy. Specifically, it involves the simultaneous qualitative and quantitative analysis of the following 10 components: gallic acid, neochlorogenic acid, chlorogenic acid, isorhamnetin-3-O-glucose-7-O-rhamnoside, rutin, luteolin, isochlorogenic acid B, isorhamnetin-3-O-glucose, glycyrrhizic acid, and kaempferol, providing a reliable and quantifiable standard for the quality assessment of throat-clearing hard candy. The chromatogram obtained by the method of this invention has the characteristics of stable baseline, high peak resolution, strong specificity, and good peak symmetry.