175 results about "Isorhamnetin" patented technology

A UGT2B inhibitor capable of increasing the bio-availability of a drug, is a compound in a free base or a pharmaceutically acceptable salt form that is selected from the group consisting of: capillarisin, isorhamnetin, β-naphthoflavone, α-naphthoflavone, hesperetin, terpineol, (+)-limonene, β-myrcene, swertiamarin, eriodictyol, cineole, apigenin, baicalin, ursolic acid, isovitexin, lauryl alcohol, puerarin, trans-cinnamaldehyde, 3-phenylpropyl acetate, isoliquritigenin, paeoniflorin, gallic acid, genistein, glycyrrhizin, protocatechuic acid, ethyl myristate, umbelliferone, PEG (Polyethylene glycol) 400, PEG 2000, PEG 4000, Tween 20, Tween 60, Tween 80, BRIJ® 58, BRIJ® 76, Pluronic® F68, Pluronic® F127, and a combination thereof. A UGT2B enhancer capable of enhancing a clearance rate of morphine-like analgesic agents, is a compound in a free base or a pharmaceutically acceptable salt form that is selected from the group consisting of: nordihydroguaiaretic acid, wogonin, trans-cinnamic acid, baicalein, quercetin, daidzein, oleanolic acid, homoorientin, hesperetin, narigin, neohesperidin, (+)-epicatechin, hesperidin, liquiritin, eriodictyol, formononetin, quercitrin, genkwanin, kaempferol, isoquercitrin, (+)-catechin, naringenin, daidzin, (−)-epicatechin, luteolin-7-glucoside, ergosterol, rutin, luteolin, ethyl myristate, apigenin, 3-phenylpropyl acetate, umbelliferone, glycyrrhizin, protocatechuic acid, poncirin, isovitexin, 6-gingerol, cineole, genistein, trans-cinnamaldehyde, and a combination thereof.

The invention discloses an artemisia sacrorum extract which contains total-flavone compounds, wherein the total-flavone compounds comprise one or more of 6-methoxy-quercetagetin-7-O-beta-D-glucoside, quercetin-3-O-beta-D-glucoside, isorhamnetin, queretagetin-3-O-beta-D-glucoside, acacetin-7-O-beta-D-glucoside, rutin, 5,7,4'-trihydroxyflavone, 3,3',4',5,7-pentahydroxyflavone, 5,7,4'-trihydroxy-3'-methoxyflavone, kaempferol, 3,5,7,4'-tetrahydroxy-6-methoxyflavone, 3',4',5,7-tetrahydroxyflavone, 5,7,4'-trihydroxy-6,3'-dimethoxyflavone, 5,7,4'-trihydroxy-6-methoxyflavone, quercitrin, 7,3',4'-trimethyl quercetin, 5-hydroxy-7,4'-dimethoxyflavone, 5,4'-dihydroxy-7-methoxyflavone genkwanin, 5,7-dihydroxy-4'-methoxyflavone, 5,7,3'-dihydroxy-4-methoxyflavone and derivatives. The artemisia sacrorum extract has remarkable prevention and treatment effects on liver injury.

The present invention relates to the active general flavone components extracted from longbract cattail as one kind of Chinese medicinal materials. The extractive includes mainly isorhamnetin, quercetin, kaempferol and their glycoside derivatives, and also includes the degraded products of the said components, their metal salt derivatives, metal complexes, etc. The extractive may be obtained via solvent extraction process and other process. The extractive has the functions of lowering blood fat, resisting atherosclerosis, raising the tolerance of cardiac and cerebral tissues to deficiency of oxygen and blood, resisting platelet aggregation, etc. and may be used in preventing and treating cardiac and cerebral vascular diseases, traumatic injury, haemorrhages and other diseases.

The invention provides a method for screening a xanthine oxidase inhibitor by ultra performance liquid chromatography and mass spectrometry. The method is used for analyzing the in-vitro inhibition rate of the extract or monomer of a natural product on the xanthine oxidase and the catalytic activity of the xanthine oxidase. By using the ultra performance liquid chromatography-mass spectrometry in the xanthine oxidase inhibitor screening, the method is rapid and accurate in sample detection, and the correlation coefficients of the linear equation reaches 0.998. The mass spectrometry has high accuracy and good specificity in detecting the mass electron ratio of a compound, and can be used for screening the xanthine oxidase inhibitor and for the kinetic study of the xanthine oxidase inhibitor without false positive and false negative results which occur in the spectrometry. The results showed that the inhibition rate I of 20 mumol/L allopurinol is 80%; the inhibition rate of 20 mumol/L isorhamnetin is 73%; the inhibition rate of 20 mumol/L genistein is 50%; and the inhibition rate of 0.1 mg/mL aqueous extract of Ginkgo biloba is 27%.

The invention provides a method for separating and purifying flavonoid glycoside compounds in lotus plumules. The method comprises the following steps of 1 preparing of a lotus plumule solution, 2 enrichment of lotus plumule total flavonoids and 3 separating and purifying through high-speed countercurrent chromatography, and quercetin-3-O-beta-D-glucoside, isorhanetin-3-O-beta-D-glucoside, apigenin-6-C-beta-D-xylose-8-C-beta-D-glucoside and apigenin-6-C-beta-D-glucose-8-C-beta-D-glucoside are obtained. The flavonoid glycoside compound monomers separated and purified through the method are high in purity, low in loss, easy to operate, high in preparation quantity, economical and environmentally friendly.

The present invention discloses a method for simultaneously preparing hyperin and isorhammetin-3-O-galactoside control products. Said method includes the following steps: (1) placing Chinese medicinal material evodia in a container, adding solvent to make extraction with reflux; (2) filtering evodia extract, reduced pressure concentrating to obtain extractum, adding water to dissolve it, then successively using petroleum ether, chloroform and ethyl acetate to make extraction; (3). using column chromatographic process to purify the ethyl acetate extracted portion twice; and (4). combining chromatographic eluents, concentrating, recrystallizing so as to obtain the invented two control products of hyperin and isorhamnetin-3-O-galactoside. Said invention is simple, and can simultaneously prepare hyperin and isorhamnetin-3-O-galactoside pure products whose purity can be above 98%.

The invention relates to a high-purity ginkgo flavone and a composition thereof. The high-purity ginkgo flavone is light brownish yellow, and tastes slightly bitter; the content of ginkgo flavone is greater than or equal to 50%, the peak ratio of quercetin to kaempferol is 0.8-1.2, the peak ratio of isorhamnetin to quercetin is greater than or equal to 0.15, the concentration of ginkgoic acid is smaller than 5ppm, and the content of residue on ignition is smaller than 0.8%. The water content is smaller than 5.0%. The method for preparing the high-purity ginkgo flavone comprises the following steps: extracting by heating ginkgo leaves, which are used as the raw material, under reflux with a 20-80 wt% ethanol solution; concentrating the extracting solution to recover ethanol; separating by passing through macroporous resin columns; carrying out ethanol desorption; diluting the product by adding water; adsorbing by passing the product through a gel-type hydrogen bond adsorption resin which is mainly based on hydrogen bond adsorption, and desorbing; and collecting the effluent liquid and the desorption liquid, concentrating and drying. The preparation technique is simple and reliable, has the advantage of low cost, and does not use toxic organic solvents in the process, thereby being environment-friendly. The high-purity ginkgo flavone can be made into a composition as an effective constituent, and the composition can be used as various preparations (capsules, tablets, oral liquids, granules, powder, injections and the like) of ginkgo flavone medicines or health products.

The invention discloses a high efficiency liquid chromatography method for simultaneously quantitatively detecting six flavonoid components in polygonum hydropiper. The method comprises the following steps of selecting a Waters chromatographic column, selecting methanol and a 0.4% formic acid water solution as flowing phases, performing gradient elution, wherein the flowing speed of the flowing phase is 1ml/min, the gradient elution is finished in 3 gradients, and the volume ratios of methanol and 0.4% formic acid water solution of 3 gradients are respectively 45:55, 55:45 and 70:30, respectively performing ultraviolet detection on eluates at 359 nm, and determining the concentration of each component. The method provided by the invention has the advantages that the method is exact and simple and convenient, the replicability is good, the method is used for measuring the contents of rutin, hyperin, quercetin, quercetinic acid, isorhamnetin and kaempferol in the polygonum hydropiper, and the reference is provided for controlling quality of a polygonum hydropiper drug.

The present invention provides one kind of UGT2B inhibitor capable of raising the bioavailability of medicine. The UGT2B inhibitor is one or the composition of capillarisin, isorhamnetin, beta-naphthoflavone and other compounds and in the form of alkali or pharmaceutically acceptable salt. The present invention also provides one kind of UGT2B promoter capable of promoting the detoxication function of liver. The UGT2B promoter is one or the composition of nordihydroguaiaretic acid, wogonin, trans-cinnamicacid and other compounds and in the form of alkali or pharmaceutically acceptable salt.

The invention relate to a ginkgo biloba leaf extract injection quality control method based on quantitative analysis of multi-components by a single marker and a fingerprint spectrum. The method comprises the steps that rutin is adopted as a reference sample, flavonols kaempferol-3-O-rutinoside, isorhamnetin-3-O-rutinoside, kaempferol-3-O-rhamnose-2-glucoside, quercetin-3-O-rhamnose-2-O-(6-O-p-hydroxy cinnamoyl) glucosyl and kaempferol-3-O-rhamnose-9-O-6-O-(p-hydroxy cinnamoyl) glucosyl are quantified through quantitative analysis of multi-components by a single marker, and the ginkgo biloba leaf extract injection batched quality stability is controlled through the fingerprint spectrum. The problem that ginkgo biloba leaf extract injection multi-component quantification and multi-index quality control lack reference samples is solved, and the quality of the ginkgo biloba leaf extract injection is ensured while the product batched quality stability is controlled through the fingerprint spectrum.

The invention discloses a ginkgo biloba extract medicinal raw material, and belongs to the field of medicines. The ginkgo biloba extract medicinal raw material is prepared from flavone and lactone which are extracted from ginkgo, wherein the flavone is prepared from quercetin, kaempferol and isorhamnetin; the lactone is prepared from bilobalide, ginkgolide A, ginkgolide B and ginkgolide C; a proportion of the flavone to the lactone is 1 to 1. According to the ginkgo biloba extract medicinal raw material disclosed by the invention, the proportion standard of the flavone and the lactone in original ginkgo biloba extract is changed, and an optimal proportion of a medicine for scavenging free radicals and resisting platelete aggregation is determined.

The invention relates to a method for determining the content of flavonoid components in folium ginkgo extract and particularly discloses a method for quantitatively determining the content of flavonoid components in folium ginkgo extract by employing multi-target ingredients. According to the method, rutin, kaempferol-3-O-rutinoside, isorhamnetin-3-O-rutinoside, kaempferol-3-O-rhamnose-2-glucoside, quercetin-3-O-rhamnose-2-O-(6-O-p-hydroxy cinnamoyl glucosyl) glucoside and kaempferol-3-O-rhamnose-9-O-6-O-(p-hydroxy cinnamoyl glucosyl) glucoside are taken as reference substances. The method for determining the content of the flavonoid components in the folium ginkgo extract is fast, simple, high in sensitivity, high in accuracy and good in stability, and is capable of accurately and comprehensively controlling the quality of the folium ginkgo extract.

The invention discloses a method for inducing flavonoids and terpene lactones to accumulate in plant leaves by using methyl jasmonate and application, and belongs to the technical field of biology. The method comprises the following step: when plant leaf cells are completely mature, spraying a 20-80mg/L methyl jasmonate solution onto the plant leaves for 1-2 times, so that a ginkgo biloba extractwith high active ingredient content can be obtained from the treated plant leaves through a conventional method. Compared with the prior art, the method has the advantages of being simple and easy toimplement, low in cost, free of pollution, stable and efficient; the flavonoids and terpene lactones obtained after regulation and control by the method disclosed by the invention are high in content;wherein the contents of quercetin, kaempferol, isorhamnetin, total flavonoids, ginkgo terpene lactone A, ginkgo terpene lactone B and total terpene lactone are respectively increased by 29.84%, 18.08%, 35.94%, 26.24%, 30.36%, 60.65% and 35.41% compared with a control group.

The present invention belongs to the technical field of gene engineering and relates to a method for obtaining yellow seed germplasm of brassica napus based on a CRISPR/Cas9 technology. A CRISPR/Cas9system is used for carrying out gene editing on a brassica napus BnMYB123 gene, so that the yellow seed brassica napus with BnMYB123 gene function deletion is obtained. The BnMYB123 has two copies inthe brassica napus, gene coding region sequences of the BnMYB123 are shown as SEQ ID NO:1 and SEQ ID NO:2, and the sequence length is 786 bp. The BnMYB123 can regulate and control synthesis and accumulation of isorhamnetin and epicatechin, thereby regulating and controlling seed coat color of the brassica napus. The method provides a brand-new way for breeding work of the yellow seed germplasm andprovides a theoretical basis for development of the brassica napus yellow seed germplasm.

The invention discloses a production method for extracting glucoside type seabuckthorn flavones from sea-buckthorn leaves and the method comprises the following steps: carrying out pre-processed machining, such as cleaning, jordaning and the like to fresh sea-buckthorn leaves or picked fallen leaves; taking ethanol as solvent; and under the action of ultrasonic wave, further breaking plant cells to release glucoside type seabuckthorn flavones-Isorhamnetin, wherein the extraction ratio is 95.6%. Compared with the traditional method, the production method disclosed by the invention has the advantages of low temperature, constant pressure, no toxic solvent residue, low energy consumption, short extraction period and high extraction efficiency, does not damage or degrade effective ingredientsand is suitable for mass production; and the additional value of agricultural products is improved.

Disclosed is a superficial venules dredging preparation for treating cerebral arteriosclerosis which comprises the constituents (by weight portion) of quercetin 2-6.35 g, kaempferide 1.5-4.6 g, isorhamnetin 0.35-1g, ginkgolides 1-3g, ginseng saponin 0.6-2g, and right amount of auxiliary materials.

The invention discloses a method for near-infrared online monitoring content change of total flavonol glycosides in a folium ginkgo extraction process. The method comprises the following steps: associating data of a quercetin content, a kaempferide content and an isorhamnetin content measured by high performance liquid chromatography with near infrared spectral information by using a partial least squares method, and establishing a multivariate quantitative calibration model of the quercetin content, the kaempferide content and the isorhamnetin content in the folium ginkgo extraction process; quickly detecting the quercetin content, the kaempferide content and the isorhamnetin content in the unknown folium ginkgo extraction process by using the established multivariate quantitative calibration model. The method disclosed by the invention can be used for sampling an extracting solution in the folium ginkgo extraction process in real time and monitoring the content change of total flavonol glycosides in the folium ginkgo extracting solution in real time; the result is accurate and reliable.

The invention discloses a bacteriostatic effective component isorhamcomin-3-O-glucoside of ageratum conyzoides and an extraction method thereof. The isorhamcomin-3-O-glucoside belongs to flavonoid glycoside compounds, has the bacteriostatic function and can be used as a contrast substance for measuring the content of the ageratum conyzoides. The invention lays the foundation for developing a novel product of an ageratum conyzoides preparation aiming at improving the quality control standard of the medicinal material of the ageratum conyzoides.

The invention discloses a method for extracting high purity seabuckthorn flavone aglycone by using seabuckthorn leaves as raw material through the steps of leaching, leach mixing, concentrating, extraction, hydrolyzing, edulcoration and dehydration, wherein the extraction agent is methanol or ethanol or the mixed liquid of methanol-water or ethanol-water, the extracting agent is cyclohexane or petroleum ether, the hydrolysate is 1-15% aqueous solution of hydrochloric acid or sulfuric acid or acetic acid or phosphoric acid.

An application of isorhamnetin and its derivatives in preparing medicines for treating cervical cancer, liver cancer, squamous cell carcinoma of tongue, lung adenocar cinoma, etc is disclosed. Said isorhamnetin and its derivatives may be isorhammetin aglycone and isohamnoside. Its advantages are high curative effect, low toxic by-effect.

The invention discloses a preparation method of flavone genins of seabuckthorn leaves, belonging to the field of the preparation method of the flavone genins. In the method, flavonoids compounds are extracted from the seabuckthorn leaves and then are added into a fluid nutrient medium inoculated with Aspergillus niger, wherein the fluid nutrient medium comprises 10-20mL of ethylene glycol, 2-4g of orange peel powder, 1-3g of (NH4)2SO4, 1-3g of bran, 0.05-0.1g of urea and 0.01-0.02g of CaCl2, and the initial pH value is 5.0-5.5; under the condition of 28-42 DEG C, the oscillation and fermentation cultivation can be carried out for 2-10 days; then, under the condition of 40-60 DEG C, the oscillating enzymolysis is carried out for 1-5h; after enzymolysis, the solution rests for centrifugation, the precipitation is dissolved by using methanol, and methanol solution containing the flavone genins can be obtained after filtration. In the invention, the source of the seabuckthorn leaves is extensive, and the biotransformation that the microorganism bacterial strain has the effect on the flavone glucoside in the raw material, therefore, the content of the flavone genins can be increased, and the contents of the three main genins of quercetin, rhizoma kaempferiae and isorhamnetin in the seabuckthorn leaves can be obviously increased.

The invention relates to the technical field of analytical chemistry and discloses a method for quickly determining the content of catechinic acid, rutin, myricetin, quercetin, kaempferol and isorhamnetin in seabuckthorn by utilizing reversed high performance liquid chromatography diode array detection. The method comprises the following steps: diluting a main component standard substance or extract of seabuckthorn flavone by using methyl alcohol, by using octadecyl silane bonded silica gel filler as a stationary phase, and using methyl alcohol-water (acidic) as a mobile phase, performing isocratic elution, and then recording a chromatogram under all the flavone absorption wavelengths. According to the method, the octadecyl silane bonded silica gel filler is used as the stationary phase and methyl alcohol-water (acidic) is used as the mobile phase, so that almost all the flavonoid aglycones components in the seabuckthorn flavone extract can be quantitatively determined, the base line separation for all the components almost can be realized and the accurate quality control on the seabuckthorn flavone extract can be effectively realized. According to the method provided by the invention, the specificity is high, the sensitivity is high and the accuracy is high.

A method for extracting refined isorhamnetin from sea buckthorn fruit paste is that: degreased sea buckthorn, which is acquired by extracting the raw material sea buckthorn, is removed of water-soluble impurities through 0-30v percent of alcohol-water solution, and the filtered residue is extracted by 60v percent of alcohol-water solution with pH value of 7-12, anhydrous methanol or anhydrous alcohol, then the extracted liquid is neutralized, steamed and re-crystallized, thereby acquiring the isorhamnetin. The invention has the advantages that: the technology is simple, the cost is low, the product is suitable for industrial production and isorhamnetin content in the product is as high as 80-95wt percent.

The present invention relates to a novel extraction technology of effective components in Chinese medicine. Specifically, the present invention relates to a novel method of using ultrasound to extract an effective component isorhamnetin in ginkgo leaves. The method uses a design program of orthogonal experiment. Based on technical parameters of the generation method, ultrasonic intensity, functional duration, functional method, solvent type, temperature of the system, and the mixing method of ultrasound through screening and extraction process, the fresh ginkgo leaves can be prepared. The ginkgo leaves are dried at a temperature of 60 DEG C for 24 hours and crushed and screened (0.45 Mu m) to be a material. The ginkgo leaves are filled into ethanol solution of 45 percent in an ultrasonic extraction reactor; the material and solution are mixed according to a ratio (Kg: L) of 1 to from 17 to 19. The generation method of ultrasound comprises a simulated audible device of ultrasound and a radial cylindrical vibration transducer. The ultrasonic power is 100W, and the ultrasonic frequency is 28 kHz. In the extraction method, the ultrasound functions once with an interval of 3 minutes; the functional time of ultrasound is 3 minutes; the total extraction time is 36 minutes; the extraction temperature is 45 DEG C; the stirring method is an air-lift stirring way; the content of isorhamnetin in the extraction solution is analyzed by using high-efficiency liquid-phase chromatogram and the quantity is fixed in a standard-material external marking way. With the methods, the extraction rate of the effective component isorhamnetin in ginkgo leaves can reach 96.77 percent.