35 results about "Isorhamnetin" patented technology

The present application belongs to the technical field of traditional Chinese medicine detection, and particularly relates to a method for determining the content of multiple flavone components in Tibetan medicine Sanwei Huangjing Tangsan, which simultaneously determines the content of three flavone aglycone, i.e., quercetin, kaempferol and isorhamnetin in Sanwei Huangjing Tangsan by using high performance liquid chromatography (HPLC). The present application can accurately determine the content of main flavone effective components in Sanwei Huangjing Tangsan, has a good linear range, high accuracy, precision and repeatability, and is stable and feasible, and can be applied to the quality inspection of Sanwei Huangjing Tangsan.

The invention belongs to the field of pharmaceutical preparations, and particularly relates to a sulfhydrylated hyaluronic acid drug-loaded hydrogel preparation and a preparation method and application thereof, and the preparation method comprises the following steps: preparing isorhamnetin nanocrystalline ISO-NCs by adopting an anti-solvent precipitation method; the preparation method comprises the following steps: constructing thiolated hyaluronic acid HA-SH responded by GSH; and uniformly embedding the ISO-NCs into the HA-SH hydrogel, so as to prepare the sulfhydrylated hyaluronic acid drug-loaded hydrogel preparation ISO-NCs-coated Gel, and preparing the sulfhydrylated hyaluronic acid drug-loaded hydrogel preparation ISO-NCs-coated Gel. The drug-loaded hydrogel preparation constructed by the invention can be used for targeted therapy of colon cancer.

The invention discloses application of isorhamnetin in preparation of antiarrhythmic drugs, and belongs to the technical field of medicines. The isorhamnetin is proposed for the first time to effectively improve myocardial conduction rate increase, conduction discrete index increase and heart rate acceleration symptoms of tachyarrhythmia patients, and the medical application of the isorhamnetin is expanded. The isorhamnetin serving as the anti-arrhythmia drug has a very good inhibition effect on increase of cAMP / PKA signal transduction pathways, realizes reversal of occurrence of arrhythmia and has important guiding significance in treatment of arrhythmia. The isorhamnetin is used as the active ingredient of the anti-arrhythmia medicine, the safety is high, the side effect is small, a new way is provided for development of the anti-arrhythmia medicine, and the application blank of the isorhamnetin in the field is filled.

This invention relates to a method for creating low-cadmium-accumulation, high-yield rice. The invention utilizes CRISPR / Cas9 technology to render the function of isorhamnetin diglyceride synthase in rice deficient, exhibiting significant advantages under cadmium stress: the mutant shows significantly higher aboveground part length, aboveground dry weight, and root length than the wild type under cadmium stress; cadmium accumulation at both the whole plant and subcellular levels is significantly reduced; and the cadmium content in grains at grain maturity is significantly lower than that of the wild type, while yield is no different from the wild type. This mutant achieves the triple goals of "cadmium stress resistance, low cadmium accumulation, and high yield and quality," reducing the risk of cadmium pollution while ensuring planting benefits, providing a new solution for safe rice production in cadmium-polluted areas.

The invention discloses a composition with a whitening effect. The composition is prepared from the following components in parts by mass: 47 to 51 parts of grape seed oil, 47 to 51 parts of pomegranate seed oil, 0.5 to 0.8 part of 4-butylresorcinol, 0.2 to 0.6 part of VC-IP (Vitamin C-IP) and 0.1 to 10 parts of isorhamnetin-3-O-neohesperidin. The invention also discloses a preparation method of the composition. The invention also discloses an essence oil containing the composition. The invention also discloses a face cream containing the composition. The invention also discloses application of the composition in preparation of cosmetics for skin whitening, spot fading and skin yellowing reduction. The composition provided by the invention establishes a multi-target synergistic pathway: 1, a blackness removal pathway for reducing the activation of oxidative stress such as ultraviolet rays on melanocytes; and 2, a jaundice removal pathway: inhibiting lipid peroxidation and promoting lipofuscin removal.

The invention relates to the technical field of biological medicines, in particular to application of isorhamnetin in preparation of a medicine for preventing and treating fatty liver diseases related to metabolic dysfunction. The invention provides application of isorhamnetin or pharmaceutically acceptable salts, esters or derivatives thereof in preparation of drugs for preventing and treating fatty liver diseases related to metabolic dysfunction. The invention also provides application of isorhamnetin or pharmaceutically acceptable salts, esters or derivatives thereof in preparation of drugs for preventing and treating diseases caused by intestinal flora disorder. The isorhamnetin can recover the diversity and richness of intestinal flora of mice with fatty liver diseases related to metabolic dysfunction and recover the balance of the intestinal flora, and the high-dose (50 mg / kg.d) donor flora transplantation of the isorhamnetin can partially reproduce metabolism improvement. The fatty liver diseases related to obesity or metabolic dysfunction are relieved through an'intestinal flora-bile acid-lipid metabolism 'axis.

The present application provides a kind of pharmaceutical composition with lipid-lowering effect, including luteolin, isorhamnetin, 9-hydroxy-4,7-megastigme-3-ketone, lotus leaf base, 3-O-feruloyl quinic acid, kaempferol-3-O-acacia disaccharide-7-O-glucoside, kaempferol-3-O-beta-D-acacia glycoside and ginsenoside Rg1.The composition of example 1 has lipid-lowering efficacy verified by golden hamster hyperlipidemia model and AML12, HepG2 cell fatty degeneration model, the regulation effect of the composition of example 1 on the key target points of AMPK signal pathway and NF-κB signal pathway is verified by using molecular pharmacology experiment, and the mechanism of the composition of example 1 in treating hyperlipidemia by regulating energy metabolism and anti-inflammatory mechanism is described.

The invention discloses a construction method of a finger-print spectrum of a styptic capsule and application of the styptic capsule in quality detection, and belongs to the field of Chinese patent medicine detection. The method comprises the following steps: firstly, adding 80% methanol solution into contents of different batches of Xuezhenning capsules for extraction to prepare Xuezhenning capsule test solution; the method comprises the following steps: taking ginsenoside Rg1, notoginsenoside R2, ginsenoside Rd, caffeic acid, isorhamnetin, verbascoside, aesculetin, ferulic acid, rutin and quercetin as reference substances, and preparing a reference substance solution; respectively injecting the test solution and the reference solution into a high performance liquid chromatograph to obtain corresponding chromatograms; performing similarity analysis on the chromatogram of the test solution; and finally, according to the chromatogram of the test solution and the chromatogram of the reference substance solution, combining a total ion chromatogram obtained by the high-resolution mass spectrum and a mass spectrum result map of the chemical components to construct the fingerprint spectrum of the styptic Ning capsule. The method can be used for simultaneously detecting 10 components in the haemostatic Ning capsule, and can be used for accurately, clearly and objectively evaluating the quality of the haemostatic Ning capsule.

The invention relates to the technical field of coreopsis tinctoria refined components and preparation and application, and provides a coreopsis tinctoria refined component and a preparation method and application thereof.The coreopsis tinctoria refined component comprises, by mass, 11 specific components of phenolic acids, flavonol, flavanol and flavonoid, and the 11 specific components comprise, by mass, 2 parts of gallic acid, 1 part of potassium sorbate, 1 part of potassium sorbate, 1 part of potassium sorbate and 1 part of potassium sorbate. The health-care food is prepared from the following raw materials in parts by weight: 3 parts of chlorogenic acid, 14 parts of quercetin, 18 parts of catechin, 5 parts of citric acid, 2-3 parts of procyanidine B, 3 parts of (+)-epicatechin, 1-31 parts of 31 parts of procyanidine C, 14 parts of rutin, 4 parts of camphanol-3-O-rutinoside and 2 parts of isorhamnetin-3-O-rutinoside. The snow chrysanthemum is Changbai Mountain snow chrysanthemum. Animal experiments show that the refined component can significantly reduce the content of total cholesterol and triglyceride in serum of a high-fat model rat, the specific component with blood fat regulating activity is prepared from Changbai Mountain snow chrysanthemum, and a new choice is provided for non-drug intervention of dyslipidemia.

The invention relates to a preparation method of a hippophae rhamnoides leaf isorhamnetin glycoside enriched solid beverage, and belongs to the field of functional plant beverage processing. The preparation method comprises the following steps: preparing sea buckthorn green tea and sea buckthorn green tea freeze-dried powder by taking sea buckthorn leaves as a main raw material; carrying out ethanol extraction and AB-8 type macroporous resin separation and purification to obtain an isorhamnetin glycoside substance enriched component; a Maillard composite system formed by whey protein isolate and maltodextrin is loaded with an isorhamnetin glycoside substance enrichment component, the isorhamnetin glycoside substance enrichment component is compounded with sea buckthorn green tea freeze-dried powder, freeze drying is performed, and the isorhamnetin glycoside enrichment solid beverage is obtained. The isorhamnetin glycoside enriched solid beverage disclosed by the invention has good solubility, and shows a remarkable immunological enhancement effect in a cyclophosphamide induced immunocompromised mouse model. The method has a wide application prospect in the aspects of high-value utilization of hippophae rhamnoides leaf resources and functional beverage development.

The invention discloses a gingko GbHCT1 gene as well as an expression protein and application thereof, and belongs to the field of plant molecular biology. The GbHCT1 gene is obtained by analyzing and screening based on data of a ginkgo transcriptome in the earlier stage of a research group, the nucleotide sequence of the GbHCT1 gene is as shown in SEQ ID NO.1, and the amino acid sequence of expression protein of the GbHCT1 gene is as shown in SEQ ID NO.2. A GbHCT1 overexpression vector is constructed and the nicotiana benthamiana is transformed, so that the content of ten flavonoid substances such as luteolin and isorhamnetin-3-O-glucoside in the transgenic GbHCT1 tobacco is remarkably increased. KEGG enrichment shows that differential metabolites are mainly enriched in a flavone synthesis pathway. The application reveals that the ginkgo GbHCT1 gene has dual effects on synthesis of flavonoid compounds by regulating carbon source distribution of a phenylpropane metabolic pathway, and a theoretical basis, a gene resource and a method are provided for secondary metabolism regulation and genetic improvement of ginkgo.

This invention discloses a method for determining the flavonoid aglycone content in acid-hydrolyzed sea buckthorn leaf extract, belonging to the field of detection technology. The invention involves acid-hydrolyzing sea buckthorn leaf extract to obtain a filtrate containing flavonoid aglycones, which is used as the test solution. High-performance liquid chromatography (HPLC) is used to detect the flavonoid aglycone content in both the test solution and the reference solution. The preparation method of the sea buckthorn leaf extract test solution rich in quercetin, kaempferol, and isorhamnetin includes: adding sea buckthorn leaf extract to methanol and hydrochloric acid solutions, mixing thoroughly, heating under reflux, cooling, shaking, and filtering to obtain the sea buckthorn leaf extract test solution rich in quercetin, kaempferol, and isorhamnetin. This invention simplifies the diverse chemical structures of flavonoid glycosides to a few flavonoid aglycones, effectively reducing dependence on expensive and scarce glycoside reference standards, simplifying chromatographic separation, and improving the stability and reproducibility of the method.

The invention provides a tobacco additive composition for preventing and treating sphagitis. The tobacco additive composition comprises isorhamnetin and naringenin. The tobacco additive has unique properties, when tobacco is ignited, isorhamnetin and naringenin are not changed through a combustion mode but are converted into a gaseous state, and the isorhamnetin and the naringenin enter the respiratory tract of the human body along with gas generated by tobacco combustion. Due to the characteristic, the traditional Chinese medicine composition can play a positive role in chronic pharyngitis and pharyngolaryngitis, effectively relieve symptoms, prevent diseases and assist the treatment process, does not cause any complications after being used for a long time, and greatly guarantees the health and safety of a user. From the perspective of use experience, the tobacco product is endowed with good taste by the additive, and the additive can be appreciated and accepted by the majority of smokers.

To provide use of isorhamnetin or pharmaceutically acceptable salts thereof in preparation of drugs for treating esophageal cancer in the technical field of biological medicines.SOLUTION: It has been found that isorhamnetin significantly inhibits malignant growth of metastatic esophageal squamous carcinoma cells and in-vivo ESCC metastatic tumors by inhibiting a fatty acid de novo synthesis pathway in tumor metabolism. Therefore, isorhamnetin can be used for developing an anti-ESCC metastatic cell growth medicine targeting an abnormally active fatty acid de novo anabolic enzyme.SELECTED DRAWING: Figure 1

The application belongs to the technical field of biological medicine, and provides a use of isorhamnetin-3-O neohesperidoside in preparation of a drug for treating alopecia, and finds that isorhamnetin-3-O neohesperidoside can promote hair growth and protect hair follicle stem cells, and as a natural extract, has many advantages such as low cost, few side effects, good patient compliance and easy to obtain, and provides a new idea for preparation of a drug for treating alopecia, in particular, a drug for treating androgenetic alopecia.

The invention discloses an EPE foamed sheet prepared from a mixed butane / HFO-1233zd composite foaming agent and a preparation method of the EPE foamed sheet, and belongs to the technical field of polyethylene foamed materials. The preparation method comprises the following steps: (1) adding low-density polyethylene, talcum powder, monoglyceride, tetra [beta-(3, 5-di-tert-butyl-4-hydroxyphenyl) propionic acid] pentaerythritol ester and isorhamnetin into an extrusion foaming machine to prepare a melt; and (2) respectively injecting mixed butane and HFO-1233zd into a third area of a machine barrel of an extrusion foaming machine, uniformly stirring the mixed butane and the HFO-1233zd with the melt, and then performing extrusion, foaming, natural cooling and cutting forming treatment to obtain the EPE foamed sheet. The residual amount of mixed butane in 48 hours of the EPE foamed sheet prepared by the invention is less than or equal to 25 ppm, and the EPE foamed sheet can be directly used subsequently.

The present application provides a kind of determination wine Jumu and ginkgo leaf content in Yining Tongmai Jian Nao Granules, adopts liquid chromatography to determine the content of monorin, strychnos seed, kaempferol, isorhamnetin, including being analyzed by liquid chromatograph, with the mixed solution of monorin control product, strychnos seed control product being prepared as a mixed control product solution, with the mixed solution of kaempferol control product, isorhamnetin control product being prepared as another mixed control product solution, with the solution of Yining Tongmai Jian Nao Granules sample being prepared as test product solution, corresponding chromatographic conditions are set respectively, are precisely sucked respectively, then are injected into liquid chromatograph respectively, and content is determined.The quality of Yining Tongmai Jian Nao Granules is evaluated by the detection of the present application by liquid chromatography, which can better reflect the internal quality of the compound granules and better control the quality of Yining Tongmai Jian Nao Granules.

The application discloses a composition with whitening effect, which comprises the following components in mass fraction: 47-51 parts of grape seed oil, 47-51 parts of pomegranate seed oil, 0.5-0.8 parts of 4-butyl resorcinol, 0.2-0.6 parts of VC-IP and 0.1-10 parts of isorhamnetin-3-O-neohesperidoside. The application further discloses a preparation method of the composition. The application further discloses an essence oil containing the composition, and a cream containing the composition. The application further discloses application of the composition in preparation of cosmetics for skin whitening, freckle lightening and skin yellowing reduction. The composition establishes multiple target point synergistic pathways: one is a black removal pathway, which reduces activation of melanocytes under oxidative stress such as ultraviolet rays; and the other is a yellow removal pathway, which inhibits lipid peroxidation and promotes lipofuscin clearance.

The invention discloses lactobacillus gasseri capable of metabolizing various components of dendrobium officinale to generate rich active small molecules to promote collagen synthesis of skin cells, and belongs to the technical field of microorganisms. The lactobacillus gasseri (Lactobacillus gasseri) CCFM1481 obtained by screening can be used for fermenting the dendrobium officinale to generate a plurality of active substances, such as quercetin, isoquercetin, p-coumaric acid, kaempferol, isorhamnetin and myricetin. The fermentation liquor obtained by fermenting dendrobium officinale with lactobacillus gasseri CCFM1481 can improve the oxidative stress state of HSF cells, promote the expression of collagen in the HSF cells and regulate cell senescence. Therefore, the lactobacillus gasseri CCFM1481 fermented dendrobium officinale fermentation liquor has a huge application prospect in preparation of products for preventing or treating skin quality.

This invention discloses a sleep-aid composition based on biotransformation technology, its preparation method, and its applications. The composition comprises jujube seed extract, sea buckthorn compound extract, γ-aminobutyric acid (GABA), and raspberry anthocyanin extract. Through a dual biotransformation process of "directional fiber-pectin dual-enzyme degradation" and "Lactobacillus plantarum-Saccharomyces cerevisiae symbiotic fermentation," the sugar chains of large molecular weight glycosides in the raw materials are broken down, efficiently enriching free small molecular weight jujube seed saponin A and isorhamnetin aglycone with high brain permeability, and achieving matrix synergy with GABA. The composition of this invention is safe and non-addictive, and can be used to prepare drugs or functional foods for sedation and hypnosis, prolonging slow-wave deep sleep, and promoting neural synapse regeneration and repair.

The invention discloses an application of isovitexin in preparation of a medicine for treating oral submucosal fibrosis (OSF). Experiments prove that isovitexin improves OSF pathological changes in a dose-dependent manner, and the effect of isovitexin is superior to that of a positive control drug tanshinone II A. The mechanism is to up-regulate Mfn2 and STX17 protein expression, enhance mitochondrial and lysosome co-localization and STX17-VDAC1 combination, down-regulate mitochondrial DNA copy number and ROS generation, and regulate mitochondrial autophagy; meanwhile, the level of p-Smad3 is reduced, the secretion of type I collagen is reduced, the activity of MMP-1 is improved, collagen deposition is reduced, and the mitochondrial membrane potential is improved. The oral coating particle prefabricated tablet (prepared from isovitexin, hydroxypropyl methyl cellulose and the like) and the gel (containing isovitexin, carbomer 940 and the like) provided by the invention are administrated through local smearing or focus injection, the mucous membrane morphology can be effectively recovered, the mouth opening degree can be improved, the safety is good, and a new choice is provided for OSF treatment.

The invention discloses a natural compound for inhibiting disulfide death by taking VAV3 as a target spot and application of the natural compound. Belongs to the technical field of biological medicine and pharmacy. According to the invention, through virtual screening and surface plasmon resonance technologies, seven small molecular compounds, including kaempferol, liensinine, sophocarpidine, isorhamnetin, deoxyshikonin, harmine and potenoside, which can be specifically bound with VAV3 protein are screened from a natural product library for the first time. In a C2C12 cell insulin resistance model, the compounds can significantly reduce the cell death rate caused by disulfide death, and the action mechanism of the compounds is to improve the activity or expression of VAV3, inhibit abnormal accumulation of cystine and improve the NADPH level, thereby effectively preventing disulfide bond crosslinking and collapse of an actin filament network. Experiments show that the compound provided by the invention can effectively inhibit disulfide death of skeletal muscle cells by targeting VAV3, and a new lead compound and a solution are provided for developing drugs for treating diabetic muscle dysfunction and related metabolic diseases.

The present application relates to the technical field of cell engineering, and particularly relates to a culture medium for producing biological antibodies and a fermentation method thereof.The culture medium comprises a basic culture medium and a fermentation culture medium; the basic culture medium is based on a commercial culture medium and comprises the following components: fumaric acid, ferric ammonium citrate, astragaloside and baicalein; the fermentation culture medium is based on a commercial culture medium and comprises the following components: L-citrulline, L-ornithine, L-theanine, L-homoarginine, L-hydroxyproline, L-allo-threonine, mannose, isorhamnetin and ginkgolin.The culture medium combination can effectively improve the expression level of target antibodies, supports cells to maintain long-term activity in a high-density state; meanwhile, the charge variant distribution of the obtained antibodies is more uniform, and the product purity and consistency are significantly improved.

The invention provides a tobacco additive, a preparation method and application of the tobacco additive, a tobacco blasting bead capsule and application of the tobacco blasting bead capsule, and a tobacco product. The preparation method of the tobacco additive provided by the invention comprises the following steps: mixing an isorhamnetin solution, a naringenin solution and an alcohol substance solution to obtain the tobacco additive, wherein an alcohol substance in the alcohol substance solution is piceatannol and / or sclareol. A tobacco additive with alcohol substances (piceatannol and / or sclareol), naringenin and isorhamnetin as main active ingredients is used as a blasting bead core material, a macromolecule hydrophobic material and a hydrophilic gel material are used as double-layer wall materials, the tobacco blasting bead capsule is prepared, after the tobacco blasting bead capsule is added into a cigarette filter, in the cigarette burning and smoking process, the blasting bead capsule is pinched to be broken, and the cigarette filter is made into the cigarette filter. The core material solution for dissolving the alcohol substances, the naringenin and the isorhamnetin forms a real-time contact environment with the smoke, and enters the smoke through diffusion and volatilization to be inhaled by a human body together with the smoke; and chronic pharyngitis and sphagitis can be relieved, prevented and treated.

The invention belongs to the technical field of cell culture, and particularly relates to application of isorhamnetin in preparation of a culture medium for promoting proliferation of mesenchymal stem cells. The concentration of isorhamnetin in the culture medium is 10-20 [mu] M. The culture medium for promoting proliferation of the mesenchymal stem cells, provided by the invention, is a growth culture medium added with a natural flavonoid compound isorhamnetin, proliferation of the adipose-derived mesenchymal stem cells can be remarkably promoted by adopting the improved culture medium for culture, and the requirement of cultured meat production on the number of seed cells is well met.

The present application provides a kind of content determination method of Fuyanxiao preparation, belong to the technical field of drug detection, the Fuyanxiao preparation is made of seven medicinal materials such as Oxalis corniculata L., Herba Patriniae, Radix Trichosanthis, Radix et Rhizoma Rhei, Cortex Moutan, Atractylodes, Linder, etc., the content determination method is using high performance liquid chromatography method same chromatographic condition is carried out content determination to 14 kinds of components in product, such as gallic acid, protocatechuic acid, epicatechin, aloe-emodin glycoside, isorhamnetin, swertisin, emodin glycoside, chrysophanol glycoside, physcion glycoside, aloe-emodin, rhein, emodin, chrysophanol, physcion.The present application increases the content determination of gallic acid, protocatechuic acid, epicatechin, aloe-emodin glycoside, isorhamnetin, swertisin, emodin glycoside, chrysophanol glycoside, physcion glycoside, aloe-emodin, rhein, chrysophanol, physcion compared with prior art, improves the requirement of product quality detection, is favorable for guaranteeing drug quality and clinical efficacy.

The invention discloses an isorhamnetin-loaded mannose modified liposome and a preparation method thereof. The liposome comprises a lipid component and an entrapped drug isorhamnetin, and the lipid component comprises soybean lecithin, cholesterol and DSPE-PEG-mannose. The liposome is prepared through the steps of lipid membrane preparation, hydration and ultrasonic treatment, extrusion and size stabilization, dialysis and purification, and freeze-drying and storage. The liposome with uniform particle size is prepared by combining a film dispersion method and an extrusion process. A compact and stable lipid bilayer is formed by soybean lecithin and cholesterol, so that the drug loading performance, the long-term storage stability and the freeze-thaw stability of the liposome are improved. Mannose modification endows the liposome with efficient and stable active targeting ability, so that target cells are accurately combined in a tumor microenvironment, and active targeting is realized. According to the invention, a collaborative optimization and quality control method taking the particle size, PDI, drug loading capacity and encapsulation efficiency as core indexes is established, so that the controllability and reproducibility of liposome quality are ensured, and the industrial development of liposome products is promoted.

The application discloses a kind of pharmaceutical composition and preparation method thereof.It is made of the following weight parts raw material components: quercetin 2-5 parts, kaempferol 1-3 parts, isorhamnetin 3-8 parts, isoginkgetin 3-8 parts, ginsenoside Rg1 1-3 parts, ginsenoside Rb1 3-8 parts, ginsenoside Rf 3-8 parts, starch 15-50 parts, calcium carbonate 10-30 parts, water 30-120 parts.The pharmaceutical composition of the application directly uses the main active components in ginseng extract and ginkgo biloba leaf extract.It is found through experimental research that the combination of isorhamnetin and isoginkgetin in the component, the combination of ginsenoside Rb1 and ginsenoside Rf has synergistic effect.