2652results about "Ketone active ingredients" patented technology

The invention discloses multifunctional hydrogel and application thereof, and belongs to the technical field of biological medicine. According to the multifunctional hydrogel, ketal mercaptan with two hydrazide ends and oxidized dextran are cross-linked to form a cross-linked network containing dynamic covalent bonds. The preparation method comprises the following steps: dissolving ketal mercaptan with two hydrazide ends in deionized water to obtain a ketal mercaptan solution, dissolving oxidized dextran in deionized water to obtain an oxidized dextran solution, mixing the ketal mercaptan solution with the oxidized dextran solution, and standing to obtain the multifunctional hydrogel. The multifunctional hydrogel has pH / ROS dual responsiveness, can respond to inflammation microenvironment and low pH of infectious wounds, remove active oxygen, release therapeutic drugs, regulate macrophage polarization and relieve bacterial infection, thereby playing a key role in inflammation, proliferation and remodeling stages of wound healing to accelerate wound healing. The curcumin-encapsulated liposome is added into the multifunctional hydrogel, so that the antibacterial effect can be further improved.

Recombinant NK cells, and especially recombinant NK-92 cells express a chimeric antigen receptor (CAR) having an intracellular domain of FcεRIγ. Notably, CAR constructs with an intracellular domain of FcεRIγ had a substantially prolonged duration of expression and significantly extended cytotoxicity over time. The CAR may be expressed from RNA and DNA, preferably as a tricistronic construct that further encodes CD16 and a cytokine to confer autocrine growth support. Advantageously, such constructs also enable high levels of transfection and expression of the recombinant proteins and provide a convenient selection marker to facilitate rapid production of recombinant NK / NK-92 cells.

Manganese-doped hollow mesoporous silica nanoparticles loaded with curcumin and an application in periodontal bone tissue repair are provided in the present disclosure, belonging to the technical field of targeted delivery of nanoparticles and bone tissue regeneration. In the present disclosure, alendronate is grafted on the surface of silica nanoparticles to achieve bone targeting, manganese is doped on the nanoparticles by a chemical method, and curcumin is wrapped in the nanoparticles.

The invention relates to the technical field of pharmaceutical preparations, and discloses a composition with liver protection and antioxidation effects and a preparation method thereof.The composition is prepared from galactosamine modified astragalus membranaceus exosome, schisandra chinensis nanocrystals, liquorice-chitosan nanoparticles, lactobacillus acidophilus freeze-dried powder, beta-(1, 3) / (1, 3, 4-tetramethyl-1, 3-pentanediol monoisobutyrate) sourced from saccharomyces cerevisiae, and beta-(1, 3, 4-tetramethyl-1, 3-pentanediol monoisobutyrate). And (6) a dextran, grape exosome-curcumin hybrid vesicle freeze-dried powder, a curcumin-mitochondrial targeting peptide compound, a resveratrol-hydroxypropyl-beta-cyclodextrin inclusion compound and reduced panthenol. According to the liver-protecting and anti-oxidation composition disclosed by the invention, multi-dimensional intervention on liver protection is realized through an elaborately designed ternary system. The system is composed of a traditional Chinese medicine extract, a microorganism-plant exosome combination and a high-bioavailability antioxidant, all the parts have a synergistic effect, and the liver protection effect is remarkably improved.

The invention relates to compositions (e.g., pharmaceutical compositions), uses, methods, non-therapeutic and therapeutic methods related to senolytic agents encapsulated or comprised in Muse cell derived extracellular vesicles (e.g. exosomes). Such compositions and methods are useful for specifically killing senescent cells and reverting any symptoms, disorders or diseases associated with senescence.

The invention discloses composite hydrogel as well as a preparation method and application thereof. The curcumin-EGCG composite hydrogel is prepared by forming a complex through hydrogen-bond interaction between gelatin and epigallocatechin gallate and carrying out chemical crosslinking through Schiff base reaction of curcumin and gelatin. The composite hydrogel disclosed by the invention has a self-healing characteristic or viscosity and a shear thinning characteristic, and is beneficial to local application. The composite hydrogel is loose and porous in structure, the drug loading rate can be increased, the drug can be slowly and controllably released, the treatment effect of the composite hydrogel in androgenetic alopecia is promoted, meanwhile, the solubility of a drug monomer can be remarkably improved, and the problem that the drug solubility is low is solved. The composite hydrogel provided by the invention provides an effective transdermal drug delivery preparation for treatment of skin diseases, and has good clinical application prospects and development values.

The invention relates to hydrogel for treating ischemic brain injury and a preparation method thereof. The hydrogel is prepared from the following raw materials: methylacryloylated hyaluronic acid, methylacryloylated gelatin, curcumin loaded HA-PPS (Hydroxypropyl-Polyphenylene Sulfite) nano-micelles and dendrobium polysaccharide loaded sodium alginate microspheres. Through combination of a nanotechnology and an intelligent response material, HA-PPS is adopted to load curcumin and sodium alginate microspheres are adopted to encapsulate dendrobium polysaccharide, so that the drug activity can be more effectively protected, the drug is protected to penetrate through a blood brain barrier and be accurately delivered to a brain injury area, and targeted release of the drug is realized; the anti-oxidation and anti-inflammatory effects are better under the combined action of the curcumin and the dendrobium polysaccharides, and meanwhile, a slow release function is also achieved.

The invention provides a composition for promoting hair regeneration after chemotherapy as well as a preparation method and application thereof, and belongs to the technical field of medicines. The preparation method comprises the following steps: modifying the surface of a gold nanocage with polydopamine, loading the modified gold nanocage on an exosome, complexing magnesium ions and zinc ions to prepare a modified exosome, preparing a nano drug-loaded liposome from curcumin, resveratrol and epigallocatechin gallate, mixing the nano drug-loaded liposome with the modified exosome to prepare a multi-layer liposome, and preparing the multi-layer liposome from the nano drug-loaded liposome. And mixing with a penetration enhancer, and adding into a temperature-sensitive hydrogel system to prepare the composition for promoting hair regeneration after chemotherapy. The composition for promoting hair regeneration after chemotherapy has a good transdermal absorption promoting effect, can slowly release drugs, promotes hair follicles to be converted from a resting stage to a growing stage under the synergistic effect of the drugs, regulates and controls multiple pathways (such as anti-apoptosis, angiogenesis promotion and anti-inflammation) at the same time, is matched with a complex pathological mechanism of hair regeneration after chemotherapy, and can promote hair regeneration after chemotherapy. Wide application prospects are realized.

The invention provides a nitrogen-doped biomass-based fluorescent carbon quantum dot, a preparation method and application, and relates to the technical field of carbon nanomaterials, the method comprises the following steps: mixing a biomass material and deionized water under an ultrasonic condition, adding a nitrogen source, and carrying out ultrasonic dispersion to obtain a uniformly dispersed to-be-reacted solution; reacting the to-be-reacted solution at a preset temperature for a preset time to obtain a mixed product solution; and filtering and dialyzing the mixed product solution to obtain the nitrogen-doped biomass-based fluorescent carbon quantum dot solution. The nitrogen-doped biomass-based fluorescent carbon quantum dot prepared by the method shows a stable fluorescent effect and good water solubility, the free radical clearance rate can reach 90% or above, and the antibacterial rate can reach 99% or above. The method can be used in the fields of drug sustained release, bioimaging and the like.

The invention relates to curcumin-loaded pectin sodium alginate gel as well as a preparation method and application thereof, and belongs to the technical field of gel preparation. The preparation method of the curcumin-loaded pectin sodium alginate gel comprises the following steps: in a dark environment, stirring and mixing curcumin, arjunic acid, ortho-aminophenol and an organic solvent, adding a polyvinyl alcohol aqueous solution, a sodium alginate solution and a pectin solution, uniformly mixing, performing ultrasonic treatment, adding calcium chloride, stirring and mixing, performing ultrasonic treatment, and performing vacuum drying to obtain the curcumin-loaded pectin sodium alginate gel. And adding oxidized hyaluronic acid, continuously stirring, stirring at normal temperature until the organic solvent is completely volatilized, and cleaning the hydrogel with deionized water to obtain the hydrogel. According to the invention, calcium ions and sodium alginate are subjected to physical crosslinking, pectin and polyvinyl alcohol are combined to form a composite hydrogel layer, the compounding of multiple components can effectively control the dissolution and drug release behaviors of active components, the bioavailability of curcumin is increased, the compactness and stability of hydrogel are increased, and the bioavailability of curcumin is improved. The oxidation resistance of the hydrogel can be effectively improved.

A traditional Chinese medicine composition for treating abnormal lung function and laryngitis caused by the influenza virus; the traditional Chinese medicine composition comprising as a primary formula of active ingredients seven medicinal / edible source materials composed of: Siraitia grosvenorii, Cinnamomum cassia, Prunella vulgaris, Houttuynia cordata, Platycodon grandiflorus, Gardenia jasminoides and Glycyrrhiza uralensis. The foundation of the primary formula is supplemented by a secondary formula of seven medicinal / edible source materials consisting of: Citrus reticulata (mandarin orange), Eriobotrya japonica leaves, Angelica dahurica, Arctium lappa root, Piper nigrum, Phragmites australis root and Syzygium aromaticum, and different preparations are prepared. The traditional Chinese medicine combined formula can be a combined formula of source herbs, and can likewise be a combined formula of extracts thereof, and can likewise be a combined formula of source herbs and extracts. The traditional Chinese medicine composition has a significant therapeutic effect on symptoms such as sore throat, itchy throat cough, swelling and pain in the throat, pharyngeal discomfort, etc. resulting from influenza-caused pneumonia such as abnormal lung function and difficulty breathing, cough, lung nodules, ground glass opacity in the lungs, pulmonary fibrosis, interstitial lung disease and laryngitis, and has a certain relieving, progression-stopping and disease condition control and treatment effects on chronic obstructive emphysema.

The invention provides a metal polyphenol network coated self-assembled nano-drug as well as a preparation method and application thereof, and belongs to the technical field of nano-drugs. The self-assembled nano-drug provided by the invention is of an organic-inorganic hybrid core-shell nano-structure; the core of the organic-inorganic hybrid core-shell nano structure is BC NPs; the BC NPs is formed by self-assembly of BBR (Boron Butadiene Rubber) and Cur (Carbon); a shell layer of the organic-inorganic hybrid core-shell nanostructure is a dynamic cross-linked network formed by self-assembly of metal ions and polyphenol through coordination. Metal ions and polyphenol are self-assembled through coordination to form a dynamic cross-linked network, and the dynamic cross-linked network coats the outer layer of the BC NPs nano-particles, so that the BC NPs nano-particles have good biocompatibility and biological safety; and the self-assembled nano-drug shows an obviously enhanced antibacterial effect under the combined treatment of NIR and H2O2.

The present invention provides information comprising at least one GSK-3 activator for use in the prevention and / or treatment of aging and aging-related conditions and diseases by increasing proteasome activity.

The invention discloses application of a compound shown as a formula I or salt or deuterated substance thereof in preparation of an anti-saccharification agent, in which ring A is a benzene ring or a five-membered ring containing O or S; the ring A may be substituted by m Ra, m being 0, 1, 2, 3 or 4; ra is hydroxyl, methyl, methoxyl, nitro or dimethylamino, and the deuterated substance is a partial deuterated substance and / or a perdeuterated substance. In the experiment of inhibiting generation of AGEs, compared with an Amino guanidine AG (Amino guanidine, AG), the anti-saccharification effect is obvious: under the low concentration, the anti-saccharification inhibition rate (the final administration concentration is 33 mu M) of the aromatic methylene acetone active small molecule compound protected by the invention is between 10% and 58%, and is far higher than the anti-saccharification inhibition rate (8.4%) of the Amino guanidine AG under the same final administration concentration (33 mu M), and the anti-saccharification inhibition rate (8.4%) of the Amino guanidine AG is far higher than the anti-saccharification inhibition rate (8.4%) of the Amino guanidine AG under the same final administration concentration (33 mu M) of the Amino guanidine AG. And the anti-saccharification inhibition rate of the radix codonopsis AG approaches to the final administration concentration (1mM) and is 57%. # imgabs0 #

The invention discloses a beta-glucuronidase inhibitor composition as well as a preparation method and application thereof. The composition is prepared from the following substances: ursolic acid, procyanidine, icariin, curcumin, epigallocatechin gallate and glycyrrhetinic acid. The beta-glucuronidase inhibitor composition has a broad-spectrum and good inhibition effect, the preparation method is simple, large-scale production is easy, and the inhibitor with a natural source is higher in use compliance, lower in toxic and side effects and more suitable for developing intestinal health products and has an application prospect.

The invention discloses a eutectic solvent for promoting ultrahigh dissolution of curcumin and a preparation method of the eutectic solvent, relates to the technical field of chemical preparations, and aims to solve the technical problem of relatively low solubility of curcumin in a solvent in the prior art. The preparation method of the eutecticevaporate solvent for promoting ultrahigh dissolution of curcumin comprises the following steps: mixing a hydrogen bond donor, a hydrogen bond acceptor and other components, and heating to a first preset temperature to obtain a clear and transparent eutecticevaporate solvent; and heating the eutectic solvent to a second preset temperature, adding curcumin powder, carrying out rapid vortex dissolution, centrifuging, and collecting a supernatant to obtain a clear and transparent high-solubility curcumin solution. According to the method, the green solvent is adopted as a medium, no additional substance needs to be added, operation is easy and convenient, the solubility of the curcumin is remarkably improved, and therefore the application range of the curcumin in the fields of cosmetics, food and pharmacy is expanded.

The invention belongs to the field of medicines, foods and health-care products, and particularly relates to an accelerant with a bone growth effect and application of the accelerant in the fields of medicines, health-care foods and functional foods. According to the application disclosed by the invention, the inhibiting effect of the morurin on osteogenesis promoting and osteoporosis resisting marker post target osteosclerosis protein is found through high-throughput virtual screening for the first time and is verified through experiments; meanwhile, in-vitro and animal experiments prove the osteogenic differentiation promoting effect of the morusin for the first time. According to the present invention, the morusin and the pharmaceutically acceptable salt thereof can be adopted as the bone growth promoter, can be singly or jointly used, can be used for promoting bone growth and resisting osteoporosis, and can be used in the forms of drugs, health-care foods, functional foods, food additives and the like.

The invention belongs to the technical field of food processing, and particularly relates to a curcumin-loaded protein-polysaccharide nanoparticle delivery system, a preparation method thereof and application of the curcumin-loaded protein-polysaccharide nanoparticle delivery system in neuroprotection. The preparation method comprises the following steps: mixing and dissolving sodium caseinate with one of glucan and pectin, hydrating at 0-4 DEG C for 12-24 hours, and performing ultrasonic and microwave treatment to obtain a pretreatment solution; adjusting the pH value, and heating the pretreatment solution to obtain covalent grafted particles; the protein-polysaccharide covalent grafted particles obtained in the second step are encapsulated with curcumin through a pH shift method, and the protein-polysaccharide-curcumin nanoparticles are obtained. The invention provides a method for carrying out Maillard reaction on sodium caseinate-polysaccharide under the assistance of ultrasound and microwaves, reaction parameters are optimized, and a preparation method of an efficient and stable curcumin delivery system and application of the curcumin delivery system in neuroprotection are constructed.

The invention belongs to the technical field of micro-nano, and particularly relates to a drug-loaded microalgae motor and a preparation method and application thereof, and the drug-loaded microalgae motor comprises a polydopamine / ferroferric oxide / chlamydomonas reinhardtii micromotor and a drug loaded on the polydopamine / ferroferric oxide / chlamydomonas reinhardtii micromotor, the drug is combined with catechol groups on the surface of polydopamine in the polydopamine / ferroferric oxide / chlamydomonas reinhardtii micromotor through covalent bonds; the medicine is an anti-cancer medicine or an anti-inflammatory medicine. The microalgae motor has the characteristics of simple preparation method, good material biocompatibility, high biodegradability and the like. The polydopamine / ferroferric oxide / chlamydomonas reinhardtii drug-loaded micromotor prepared by the invention has the characteristic of self-driving in intestinal juice, so that the polydopamine / ferroferric oxide / chlamydomonas reinhardtii drug-loaded micromotor can target an intestinal disease part under the guidance of an external magnetic field to improve the treatment effect and reduce the side effect of a drug, thereby expanding the application space of a micro-nano motor in the field of biomedicine.

The invention discloses a step-by-step targeting placenta trophoblast micro-nano robot as well as a preparation method and application thereof. The micro-nano robot comprises a yeast cell wall of a bag-shaped structure, bio-enzyme unevenly distributed on the surface of the yeast cell wall and targeted placenta trophoblast drug-loaded nanoparticles located in the yeast cell wall. The surfaces of the targeted placenta trophoblast drug-loaded nanoparticles are connected with placenta-like chondroitin sulfate A binding polypeptides. Inflammation placenta tissue can be targeted through macrophages, the medicine is accurately delivered to placenta trophoblastic cells through the medicine-carrying nano-particles connected with the targeting peptide, step-by-step accurate targeting is achieved, the medicine does not penetrate through the placenta barrier to reach the body of a fetus, and high biological safety is achieved for pregnancy administration.

A magnetite-based micro / nanorobot and a preparation method and use thereof are provided. The magnetite-based micro / nanorobot uses a polydopamine-coated magnetite as a carrier that is loaded with an anti-inflammatory drug and is finally coated with sodium alginate. The magnetite-based micro / nanorobot of the present disclosure improves a loading effect of the drug resveratrol and has an excellent anti-inflammatory effect. The magnetite-based micro / nanorobot of the present disclosure does not have obvious cytotoxicity, and exhibits excellent biocompatibility and high safety performance. The magnetite-based micro / nanorobot of the present disclosure allows the responsive release according to different pH values of a gastrointestinal environment. Compared with the traditional drug carriers, the magnetite-based micro / nanorobot of the present disclosure has a high rate and prominent targetability, and can be prepared by a method involving simple steps and easy operations.

The invention belongs to the technical field of biological medicines, and particularly relates to application of oxibenzophenone in preparation of a medicine for treating pathological cardiac hypertrophy and / or heart failure. Research results in myocardial cells of primary newborn rats show that Exibenzophenone can inhibit pathological hypertrophy of myocardial cells induced by phenylephrine (PE). Animal experiments show that the epoxybenzophenone can improve cardiac dysfunction induced by aortic arch constriction (TAC) and reduce cardiac hypertrophy and cardiac tissue fibrosis. The invention provides a new drug research and development approach and a drug action target for treating pathological cardiac hypertrophy and heart failure, and has very important medicinal value.

The invention discloses protein-based nanoparticles as well as a preparation method and application thereof. The protein-based nanoparticles have a core-shell structure, the core is zein particles loaded with active drugs, and the shell comprises a trypsin inhibitor. The preparation method of the protein-based nanoparticles comprises the following steps: 1) preparing a zein dispersion liquid loaded with an active drug and a shell material aqueous solution; and 2) slowly adding the zein dispersion liquid loaded with the active drug into the shell material aqueous solution in a stirring state, and then carrying out rotary evaporation and freeze drying. The protein-based nano-particle has an excellent gastrointestinal digestion resisting effect, various hydrophobic active drugs can be effectively delivered to a lower digestive system, the stability and slow release efficiency of the loaded drugs are remarkably enhanced, and the protein-based nano-particle is good in biocompatibility, safe, non-toxic, simple in preparation process and suitable for industrial production. The method is suitable for large-scale industrial production and application.

The invention relates to a curcumin-loaded MMP response type melittin nano-pellicle vesicle as well as a preparation method and application thereof, and belongs to the technical field of pharmaceutical preparations. The preparation method comprises the following steps: firstly, preparing curcumin entrapped lipidosome; then carrying out culture amplification on engineering bacteria carrying melittin recombinant plasmids, extracting cell membranes after removing cell walls, and carrying out ultrasonic treatment and membrane extrusion to obtain melittin cell membrane nano-vesicles; fusing the curcumin lipidosome and the cell membrane nano-vesicles in an ultrasonic extrusion mode to obtain fused vesicles; and finally, carrying out surface calcium phosphate mineralization treatment on the fused vesicles to obtain the curcumin-loaded MMP response type melittin nano pellicle vesicles. The nano mycofilm vesicle prepared by the invention can be used for preparing antitumor drugs, and the biocompatibility and in-vivo stability of nanoparticles are improved by introducing a calcium phosphate coating; through combined delivery of melittin and curcumin, the anti-tumor effect is enhanced, so that the growth and proliferation of tumor cells are inhibited.

The present invention discloses an amorphous curcumin nanoparticle and its preparation method and application. In the present invention, an absolute ethanol solution of curcumin and apple polyphenol is dropped into water under stirring, and then left standing for treatment to remove ethanol and adjust the pH to obtain amorphous curcumin nanoparticles. Through the bottom-up antisolvent precipitation method and the interaction between curcumin and polyphenol, and by optimizing the important conditions in the preparation process, the present invention effectively reduces the particle size of curcumin and transforms its crystal structure into an amorphous structure. At the same time, the amorphous curcumin nanoparticles can be used as an emulsifier for Pickering emulsions to prepare curcumin emulsions. Compared with curcumin nanoparticles, the curcumin emulsions have higher stability. The present invention has low energy consumption, reduced particle size, can improve solubility, enhance its bioavailability, improve its emulsifying properties, and has good application prospects.

The invention discloses an application of phloretin in preparation of a medicine or a feed additive for treating or preventing swine envelope virus infection, the medicine is phloretin or oral liquid, granules, tablets and powder prepared from phloretin as an effective component and a solvent or an auxiliary material, and the use dosage is 2.5-10mg / kg of the body weight of a pig; or the phloretin is directly added into the feed, and the addition amount of the phloretin is 0.01-0.03% of the total weight of the pig feed. The phloretin or the composition containing the phloretin is used as a medicine or a feed additive, is high in safety and free of toxic and side effects, and has the effects of remarkably inhibiting replication of envelope viruses such as porcine epidemic diarrhea virus, porcine reproductive and respiratory syndrome virus and African swine fever virus and reducing the toxicity of the porcine envelope viruses; the defects of immune failure possibly caused by vaccination, virus variation caused by use of antiviral drugs and the like are overcome, the morbidity and mortality of the piglets infected with the swine envelope virus are effectively reduced, and the effect of preventing and treating or preventing the swine envelope virus infection diseases is achieved.

The invention provides an autologous fat-derived mesenchymal stem cell composition as well as a preparation method and application thereof, and belongs to the technical field of medical auxiliary materials. The method comprises the following steps: separating mesenchymal stem cells from autologous fat particles, culturing in a stem cell culture medium added with cell stimulation particles and fetal calf serum, separating to obtain stem cells and an extracellular matrix, preparing the stem cells into stem cell lipidosome, and preparing the stem cell lipidosome into the mesenchymal stem cells. Sanguinarine and curcumin are embedded in a composite shell material of chitosan and beta-cyclodextrin to prepare the sustained release microspheres. The mesenchymal stem cell composition of the autologous fat source prepared by the invention has good air permeability, softness, certain mechanical property and good biocompatibility, can quickly promote wound healing, is convenient to remove, and does not cause secondary injury.

The invention discloses a targeted nano delivery system based on curcumin (Cur) and near-infrared aggregation-induced emission (AIE) molecule BDTA and application of the targeted nano delivery system in treatment of glioblastoma (GBM), and belongs to the field of biomedicine. The nano platform is composed of nanoparticles (MPMs (at) Cur-BDTA NPs) modified by a macrophage membrane, curcumin and BDTA are combined by adopting a coprecipitation method, and the targeting property of the nano platform to tumors is enhanced through modification of the macrophage membrane. The nanometer platform combines the functions of photo-thermal therapy (PTT), photodynamic therapy (PDT) and chemotherapy, and can achieve multiple treatment effects. The curcumin improves PTT sensitivity by down-regulating expression of heat shock protein HSP-70 in the platform, and effectively improves photothermal conversion efficiency (87.6%) and singlet oxygen generation amount (1.7 times higher than that of a control group) through a Foster resonance energy transfer (FRET) mechanism. In a glioblastoma in-vitro experiment, the nano platform shows a synergistic treatment effect of photo-thermal, photodynamic and chemotherapy. The invention provides an intelligent treatment platform integrating photo-thermal treatment, photodynamic treatment and chemotherapy, and provides an efficient and safe innovative treatment strategy for precise treatment of glioblastoma.

The invention relates to the technical field of medicines, in particular to a curcumin-loaded engineered cell membrane nano-vesicle NVs-CUR and a preparation method thereof, and the preparation method comprises the following steps: S1, extruding a macrophage suspension through an extruder to obtain an NVs resuspension; and S2, adding the CUR mixed solution into the NVs resuspension solution, and carrying out ultrasonic treatment to obtain the nano vesicle NVs-CUR. As a novel drug delivery system, the NVs-CUR shows excellent performance in the aspects of physical stability, drug release and stability, cellular uptake ability, cytotoxicity and the like. The characteristics provide powerful support for application in treatment of diseases such as nerve injury and the like, and indicate the wide prospect in the field of biomedicine.

The invention relates to a gingerol and shogaol loaded double-layer microneedle and a preparation method and application thereof.The double-layer microneedle comprises a microneedle base and a microneedle body, and the microneedle body is prepared from gingerol, shogaol, a base material and a medicinal solubilizer. The double-layer microneedle provided by the invention realizes simultaneous loading of gingerol and shogaol, solves the problems of poor stability and low water solubility of gingerol and shogaol, and realizes transdermal transmission of drugs. The gingerol and the enol are jointly loaded in the microneedle body of the double-layer microneedle, and the gingerol and the enol have a synergistic effect, so that the treatment effect of the microneedle on the diabetic wound surface can be further improved.