The invention belongs to the field of natural
organic chemistry, and relates to a purifying method for
schisandrin, and particularly to a method for purifying
schisandrin in
schisandra chinensis by using
microwave countercurrent extraction,
macroporous resin adsorption, and
column chromatography purification. The method has the following advantages that: 1, the
microwave extraction is adopted, and
ethanol is adopted as a
solvent to remove part of fatty oil so as to greatly reduce the extraction time, and improve production efficiency, in addition, the method is simple; 2, the
macroporous resin can be conveniently and rapidly regenerated, and is suitable for large-scale production, water
elution can remove most of pigments and impurities, and the
macroporous resin is easy to operate; 3, the macroporous resin and the
silica gel column chromatography are combined so as to provide a good separation and purification effect, at the
late stage, recrystallization is adopted so as to greatly improve the product purity. With the present invention, the
microwave countercurrent extraction, the macroporous resin separation and purification and the
column chromatography are adopted to carry out further purification, such that the product purity is greatly improved, and the semi-finished product
schisandrin with the content more than 65% and the final product schisandrin with the content more than 98% are obtained.