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Method for measuring content of salvianolic acid B and schisandrin in heart-benefiting pulse-invigorating granule by quantitative analysis of multi-components by single marker(QAMS)

A technology of schisandrin methyl and salvianolic acid, which can be used in measurement devices, instruments, scientific instruments, etc., can solve the problems of long inspection cycle, time-consuming, and rare reference substances.

Active Publication Date: 2016-08-03
TIANJIN TASLY PHARMA CO LTD +1
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

Simultaneous determination of several components in traditional Chinese medicinal materials by traditional determination methods not only faces the difficulty of reference substances, but also requires a long test cycle, and consumes energy and time.

Method used

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  • Method for measuring content of salvianolic acid B and schisandrin in heart-benefiting pulse-invigorating granule by quantitative analysis of multi-components by single marker(QAMS)
  • Method for measuring content of salvianolic acid B and schisandrin in heart-benefiting pulse-invigorating granule by quantitative analysis of multi-components by single marker(QAMS)
  • Method for measuring content of salvianolic acid B and schisandrin in heart-benefiting pulse-invigorating granule by quantitative analysis of multi-components by single marker(QAMS)

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0152] Take Yixin Fumai Granules numbered as Test 1, and measure the contents of salvianolic acid B and schisandrin A.

[0153] Step 1: Preparation of Schizandrin A Reference Substance Solution

[0154] Take an appropriate amount of schisandrin A reference substance, accurately weigh it, add 70% methanol to make a solution containing 0.036 mg of schisandrin A per 1 ml, and obtain it.

[0155] Step 2: Preparation of Yixin Fumai Granules Test Solution

[0156] Take an appropriate amount of Yixinfumai granule, grind it finely, take about 1.0g, accurately weigh it, add 10ml of 70% methanol to ultrasonically dissolve it for 30 minutes, take it out, place it at room temperature, make up the lost weight with 70% methanol, shake well, centrifuge, and pass through 0.45 Filter through a microporous membrane of μm, and take the filtrate.

[0157] Step 3: Inject the Schizandrin A reference substance solution and the test solution into the high-performance liquid chromatograph respective...

Embodiment 2

[0161] Take Yixin Fumai Granules numbered as Experiment 2, and measure the contents of salvianolic acid B and schisandrin A.

[0162] Step 1: Preparation of Schizandrin A Reference Substance Solution

[0163] Take an appropriate amount of schisandrin A reference substance, accurately weigh it, add 70% methanol to make a solution containing 0.030 mg of schisandrin A per 1 ml, and obtain the product.

[0164] Step 2: Preparation of Yixin Fumai Granules Test Solution

[0165] Take an appropriate amount of Yixinfumai granules, grind them finely, take about 1.0g, weigh them accurately, add 5ml of 70% methanol to dissolve them by ultrasonication for 20 minutes, take them out, place them at room temperature, make up the lost weight with 70% methanol, shake well, centrifuge, and pass through 0.45 Filter through a microporous membrane of μm, and take the filtrate.

[0166] Step 3: Inject the Schisandrin A reference substance solution and the test solution into the high-performance li...

Embodiment 3

[0170] Take Yixin Fumai Granules numbered as Test 3, and measure the contents of salvianolic acid B and schisandrin A.

[0171] Step 1: Preparation of Schizandrin A Reference Substance Solution

[0172] Take an appropriate amount of the reference substance of schisandrin A, accurately weigh it, add 70% methanol to make a solution containing 0.040 mg of schisandrin A per 1 ml, and obtain it.

[0173] Step 2: Preparation of the test solution

[0174] Take an appropriate amount of Yixinfumai granules, grind them finely, take about 1.0g, weigh them accurately, add 20ml of 70% methanol to ultrasonically dissolve them for 40 minutes, take them out, place them at room temperature, make up the lost weight with 70% methanol, shake well, centrifuge, pass through 0.45 Filter through a microporous membrane of μm, and take the filtrate.

[0175] Step 3: Inject the Schizandrin A reference substance solution and the test solution into the high-performance liquid chromatograph respectively,...

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Abstract

The invention relates to a method for measuring content of salvianolic acid B and schisandrin in heart-benefiting pulse-invigorating granule by a quantitative analysis of multi-components by single marker(QAMS). The method comprises the following steps: a tested object solution of heart-benefiting pulse-invigorating granule and a reference solution of schisandrin are prepared, then the materials are respectively injected into a high-performance liquid chromatography, gradient elution is carried out under specific chromatogram condition, the content of the schisandrin is determined, through the quantitative analysis of multi-components by single marker, and the content of the salvianolic acid B in heart-benefiting pulse-invigorating granule is calculated by using relative correction factors between the salvianolic acid B and the schisandrin. The established quantitative analysis of multi-components by single marker can increase a quality control level of the product, and at the same time, the detection cost is saved.

Description

technical field [0001] The invention relates to a drug analysis and detection method, in particular to a method for determining the contents of salvianolic acid B and schisandrin A in Yixin Fumai granules by using a one-test and multiple-evaluation method. Background technique [0002] Yixin Fumai Granule is a drug produced by Tianjin Tasly (Liaoning) Pharmaceutical Co., Ltd. Its main functions are to nourish qi and nourish yin, activate blood and restore pulse; it is used for deficiency of both qi and yin, internal resistance of heart and blood, chest pain, chest tightness and discomfort , palpitations and pulse knots. It has the characteristics of high drug loading, fast onset, no peculiar smell, and easy to carry. The preparation is a compound preparation composed of 6 medicinal materials: sun-dried ginseng, astragalus, radix radix, schisandra chinensis, salvia miltiorrhiza, and chuanxiong. For this product, the existing detection method only includes traits, 2 color ide...

Claims

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Application Information

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IPC IPC(8): G01N30/02G01N30/06
Inventor 侯春莲刘海涛高展阚红玉孙玉侠曹凤兰徐立元赵光燃杨建会张金巍杨雪梅
Owner TIANJIN TASLY PHARMA CO LTD
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