The invention provides an HPLC-QAMS (
High Performance Liquid Chromatography-Quantitative Analysis
Mass Spectrometry) synchronous quantitative detection method for four rare ginsenosides in cooked pseudo-
ginseng, and belongs to the technical field of
traditional Chinese medicine detection. According to the method,
ginsenoside 20 (R)-Rg3 is taken as an internal reference, an HPLC-QAMS (
High Performance Liquid Chromatography-Quantitative Amplified
Mass Spectrometry) method is adopted, and simultaneous quantification of four ginsenosides is realized through verified
relative correction factors; rSDlt; the method has the advantages of high accuracy (the
recovery rate is 91.95-101.34%, RSDlt, 1.8%), high
linearity (Rgt, 0.9997) and high stability (RSDlt, 3.5%), and the reference standard cost can be reduced by 75%. The QAMS method is excellent in green analysis
score and blue applicability grade index. By analyzing 15 batches of samples, the consistency (relative to ESM, RE% lt, 5%) of the samples is confirmed, and the transfer rate of 20 (R) G-Rg3 is 85.82% by optimizing the extraction condition.