The invention relates to a quality evaluation method and application of a wild chrysanthemum flower medicinal material. According to the method, a fingerprint spectrum of the wild chrysanthemum flowermedicinal material is constructed, the fingerprint spectrum is analyzed through a mode recognition method, and the qualitative and quantitative information of multiple components in the wild chrysanthemum flower medicinal material in a once-detection-multi-evaluation method is reflected comprehensively. The method comprises the following steps: preparing a mixed reference solution and a test solution, then respectively detecting the mixed reference substance solution and the test solution by adopting high performance liquid chromatography, recording fingerprint spectrums of the mixed reference substance solution and the test solution, recording the peak areas of the 12 components by adopting the once-detection-multi-evaluation method, calculating a relative correction factor, and calculating the contents of the 12 components by using the correction factor. According to the method, the contents of the 12 components in the wild chrysanthemum flowers are simultaneously determined by highperformance liquid chromatography, operation is simple and convenient, the reproducibility is high, multiple index components in the wild chrysanthemum flowers are quantified rapidly and accurately at the same time, the separation degree of the 12 components is high, and a theoretical basis is laid for quality control of the wild chrysanthemum flowers.