Method for detecting compounds in toad venom

Abstract

The invention discloses a method for detecting compounds in a toad venom. The method for detecting the compounds in the toad venom comprises the steps of the preparation of a reference solution, the preparation of a test solution, determination of chromatographic conditions, determination of relative correction factor, determination of component content of the test solution, and the like. Cinobufagin is selected as an internal standard substance. The method for detecting the compounds in the toad venom not only solves a problem that a reference product is expensive and rare, but also complieswith a principle of environmentally friendly and green Chinese medicine, and the cinobufagin with high content, stable peak shape and low price is selected as the internal standard substance. At the same time, the content of six compounds in the toad venom is measured, the detection efficiency is greatly improved and the quality of the toad venom is comprehensively controlled. According to a detection object to determine a reasonable mobile phase, a chromatographic column, an elution procedure, a detection wavelength, a column temperature and other chromatographic conditions, under the selected chromatographic conditions, the content of the compounds in the toad venom can be quickly determined, precision is high, reproducibility is good, stability is good, recovery rate is high and measurement results are accurate .

Description

technical field [0001] The invention relates to a method for detecting compounds in toad venom. Background technique [0002] Toad venom is a nationally protected medicinal material, and it is the dry product of the white secretion of the national "three haves" animal toad's auricular gland. It has the functions of detoxification, pain relief, and refreshing. Toad Su is mainly used to treat carbuncle, ulcer, sore throat, heat stroke, coma, abdominal pain, vomiting and diarrhea. There are 88 kinds of prescriptions using Toad Su as medicine, the quality of which directly affects the efficacy of the preparation. At present, the evaluation standard of Chansu is mainly based on the content index of each single component to characterize its quality. The external standard method that has been widely used requires multiple standards to conduct corresponding analysis on multiple components in the medicinal material. This method Long time consumption, high cost of standard products a...

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Problems solved by technology

At present, the evaluation standard of Chansu is mainly based on the content index of each single component to characterize its quality. The external standard method that has been widely used requires multiple standards to conduct corresponding analysis on multiple components in the medicinal material. This method It takes a long time, the high cost of standard products and the high purity of standard products limit its application

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