Method for detecting compounds in toad venom

A detection method and compound technology, applied in measuring devices, instruments, scientific instruments, etc., can solve the problems of high cost of standard products, limitation of high-purity factors, long time consumption, etc., and achieve high precision, improve detection efficiency, and high recovery rate. Effect

Inactive Publication Date: 2018-12-11
山东宏济堂制药集团股份有限公司
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Problems solved by technology

At present, the evaluation standard of Chansu is mainly based on the content index of each single component to characterize its quality. The external standard method that has been widely used requires multiple standard

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  • Method for detecting compounds in toad venom
  • Method for detecting compounds in toad venom
  • Method for detecting compounds in toad venom

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Embodiment Construction

[0016] In order to clearly illustrate the technical features of this solution, the present invention will be described in detail below through specific implementation modes and in conjunction with the accompanying drawings.

[0017] 1 Instruments and reagents:

[0018] Control substances: sandbufingin, bufabufingin, bufofalin, bufalin (purchased from Tianjin Vientiane Hengyuan Technology Co., Ltd.), cinobufagin and esterbufagenin (purchased from China Food and Drug Certification Institute);

[0019] Test product: 41 samples of toad venom;

[0020] Instruments: Agilent 1260 HPLC system, Waters 2690 HPLC system;

[0021] Reagents: acetonitrile (Merck, chromatographically pure), formic acid (Merck, chromatographically pure), pure water;

[0022] 2 Methods and results:

[0023] 2.1 Preparation of reference solution:

[0024] Using methanol as the solvent, accurately weigh 10 mg of each of the reference substances of sabubufolin, bufabufagin, bufafetalin, bufafolin, cinobufafo...

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Abstract

The invention discloses a method for detecting compounds in a toad venom. The method for detecting the compounds in the toad venom comprises the steps of the preparation of a reference solution, the preparation of a test solution, determination of chromatographic conditions, determination of relative correction factor, determination of component content of the test solution, and the like. Cinobufagin is selected as an internal standard substance. The method for detecting the compounds in the toad venom not only solves a problem that a reference product is expensive and rare, but also complieswith a principle of environmentally friendly and green Chinese medicine, and the cinobufagin with high content, stable peak shape and low price is selected as the internal standard substance. At the same time, the content of six compounds in the toad venom is measured, the detection efficiency is greatly improved and the quality of the toad venom is comprehensively controlled. According to a detection object to determine a reasonable mobile phase, a chromatographic column, an elution procedure, a detection wavelength, a column temperature and other chromatographic conditions, under the selected chromatographic conditions, the content of the compounds in the toad venom can be quickly determined, precision is high, reproducibility is good, stability is good, recovery rate is high and measurement results are accurate .

Description

technical field [0001] The invention relates to a method for detecting compounds in toad venom. Background technique [0002] Toad venom is a nationally protected medicinal material, and it is the dry product of the white secretion of the national "three haves" animal toad's auricular gland. It has the functions of detoxification, pain relief, and refreshing. Toad Su is mainly used to treat carbuncle, ulcer, sore throat, heat stroke, coma, abdominal pain, vomiting and diarrhea. There are 88 kinds of prescriptions using Toad Su as medicine, the quality of which directly affects the efficacy of the preparation. At present, the evaluation standard of Chansu is mainly based on the content index of each single component to characterize its quality. The external standard method that has been widely used requires multiple standards to conduct corresponding analysis on multiple components in the medicinal material. This method Long time consumption, high cost of standard products a...

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Application Information

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IPC IPC(8): G01N30/06G01N30/74
CPCG01N30/06G01N30/74
Inventor 罗毅孙崇峰范圣此孟兆青常鸿彦刁瑞丽李樱
Owner 山东宏济堂制药集团股份有限公司
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