37 results about "Toad" patented technology

The utility model relates to the technical field of toad hatching, and provides a toad egg zone indoor hatching pond which comprises a pond body, two symmetrically-arranged vertical plates are fixedly connected to the upper end of the pond body, a throwing box is arranged between the two vertical plates, and a moving mechanism used for moving the throwing box is arranged between the two vertical plates. The moving mechanism comprises a reciprocating lead screw rotationally connected between the two vertical plates, a fixing block is fixedly connected to the upper end of the feeding box, the reciprocating lead screw penetrates through the fixing block and is matched with the fixing block, and a stirring mechanism used for stirring materials is arranged in the feeding box. A limiting mechanism used for limiting the throwing box is arranged between the two vertical plates. By arranging the moving mechanism, when a nutrient solution is fed, the feeding box moves in a reciprocating mode, the electromagnetic valve is opened, the nutrient solution is fed out, feeding can be completed in the moving process, and feeding is more uniform.

This invention belongs to the field of traditional Chinese medicine, specifically relating to a topical delay spray and its preparation method. The invention uses the dried epidermis of the Huaxi toad (with the toad venom removed), and extracts it efficiently through a combination of water and alcohol extraction. Male silkworm moth oil can nourish and strengthen the body, tonify the liver and kidneys, and combat fatigue; studies have shown that male silkworm moth oil can effectively treat kidney yang deficiency. This invention combines the synergistic effects of toad skin extract and male silkworm moth oil to prepare a delay spray that can significantly treat premature ejaculation and erectile dysfunction in men. This preparation has the advantages of direct action, sustained release, avoidance of drug contact, and simple operation. This invention further improves the rational utilization system of toad resources, provides a practical solution for the efficient development of toad medicinal resources, and significantly reduces production and clinical application costs.

The utility model relates to the technical field of toad breeding pools, in particular to a toad breeding pool structure which comprises a breeding pool, a cleaning component is installed on the inner wall of the breeding pool, a bearing component is installed at the top end of the breeding pool in a suspended mode, the bearing component comprises a shadow table, and inserting plates are inserted into the left side and the right side of the shadow table through through grooves. The tail end of the insertion plate is connected with a bearing plate, and a suspension part is arranged at the center of the top end of the shadow table. The hanging beam plates are erected on the two sides of the top end of the culture pond, the shadow table is arranged below the hanging beam plates, the insertion plates are pulled on the sides of the shadow table, then the bearing area of the bearing plate is changed, the insertion plates can be fixed through the fixing bolts, and a water surface rest platform can be provided through cooperation of the bearing plate and the shadow table; and the hanging beam plate can be used for shading partial area of the shadow table, so that more selection spaces are provided for toads.

The utility model belongs to the technical field of agricultural production auxiliary equipment, disclose a kind of sugarcane toad trapping system, it includes luring unit, treaded trap assembly, conveying pipeline, anti-back climbing device and collection device;Luring unit is arranged in the central region of treaded trap assembly, for attracting sugarcane toad to go to the position of treaded trap assembly;Treaded trap assembly bottom is communicated with conveying pipeline, for falling into conveying pipeline when sugarcane toad treads;Anti-back climbing device two ends are respectively communicated with conveying pipeline and collection device, for guiding sugarcane toad one-way into collection device and preventing back climbing.The utility model system is simple, easy to build, and high capture efficiency can provide continuous, efficient prevention and control effect, and will not destroy ecological environment, good reliability, strong practicality.

The present invention provides a method for quantitatively detecting the blood-transmitting components of Toad Venenum or Salvia miltiorrhiza in Musk Tongxin Dropping Pills, specifically comprising ultra-high performance liquid chromatography-tandem mass spectrometry detection of drug-containing plasma or tissue. The liquid chromatography conditions employ a C18-filled column, mobile phase A consisting of 0.05% to 0.2% formic acid in water, mobile phase B consisting of 0.05% to 0.2% formic acid in acetonitrile, and gradient elution; an electrospray ion source in positive ion mode, a drying gas temperature of 300 to 400°C, a drying gas flow rate of 4 to 8 L / min, a sheath gas temperature of 300 to 400°C, a sheath gas flow rate of 8 to 12 L / min, and a nebulizing gas pressure of 40 to 50 psi; a capillary inlet voltage of 3 to 4 kV; a cone voltage of 450 to 550 V, a collision energy of 10 to 30 eV, and a fragmentation voltage of 100 to 190 V. The present invention can simultaneously and quantitatively detect 9 kinds of toad venom components and 3 kinds of salvia miltiorrhiza components entering the blood in Musk Tongxin Dropping Pills, and has high detection sensitivity, good stability and short detection time, and is suitable for the pharmacokinetic study of Musk Tongxin Dropping Pills.

The ecological toad culture pond comprises a pond body, water tanks are formed in the two sides in the pond body respectively, shallow grass layers are arranged on the peripheries of the water tanks, intercepting nets are arranged in the water tanks, lifting frames are arranged on the two sides of the intercepting nets respectively, the lifting frames are connected with mounting bases, supporting frames are inserted into the mounting bases, and the supporting frames are connected with the water tanks through the lifting frames. The two sides of the pool body are respectively provided with a lifting mechanism, the two lifting mechanisms are respectively connected with the corresponding supporting frames, a sunshade inhabitation area is arranged between the water tanks on the two sides, and a boss is arranged on one side in the pool body, so that toad cortex naturally falling off from toads can be effectively intercepted and collected, the toad cortex is prevented from being lost along with water flow, and the toad cortex is prevented from falling off. In addition, the sun-shading inhabiting area is arranged, so that direct strong light can be effectively blocked, a proper environment is created for the toads, abnormal ecdysis of the toads caused by strong light stress is reduced, and the toad cortex quality is effectively guaranteed.

The utility model discloses an indoor soilless underwater hibernation pool for toads, which relates to the technical field of toad cultivation and is technically characterized by comprising a pool body, a cover plate is slidably mounted on the upper end face of the pool body, a support plate is fixedly mounted on the outer wall of the pool body, an opening and closing mechanism is arranged on the upper end face of the support plate and comprises a moving component and an overturning component, and the moving component is connected with the overturning component. The moving assembly comprises an electric push rod fixedly installed on the upper end face of the supporting plate, a moving block is fixedly installed at the telescopic end of the electric push rod, a toothed plate is fixedly connected to the outer wall of the moving block through a fixing rod, the overturning assembly comprises a connecting frame fixedly installed on the outer wall of the pool body, and a rotating shaft is rotationally installed in the connecting frame; a gear meshed with a toothed plate is fixedly mounted on the outer wall of the rotating shaft, the overturning assembly can drive the cover plate to be opened and closed under the driving of the moving assembly, and the opening and closing degree of the cover plate can be adjusted by adjusting the extending position of the electric push rod so as to maintain a proper temperature environment in the pool body.

The scheme discloses an alkaloid compound with 14, 21 oxygen bridge structures and a preparation method and application thereof in the field of traditional Chinese medicine extraction and separation. The ethyl acetate extraction part of ethanol extracts of toad skin is repeatedly separated and purified by combining various chromatography technologies; 1D and 2D nuclear magnetic and high resolution mass spectrometry are used to identify the structures of two new compounds, and the molecular formula and structure formula of the two new compounds are deduced. In vitro pharmacodynamic studies show that the two alkaloid compounds with 14, 21 oxygen bridge structures have good inhibition on colon cancer cells (HCT-116), and IC 50 The two alkaloid compounds with 14, 21 oxygen bridge structures have good inhibition on colon cancer cells (HCT-116), and IC 50 values are 0.92 μM and 10.91 μM respectively. The present application is helpful for the development and utilization of natural compounds from toad.

1. Name of the designed product: ring (Wangcai golden toad). 2. Use of the designed product: jewelry. 3. Design points of the designed product: in shape. 4. Picture or photo that best shows the design points: perspective view.

The utility model discloses a toad specification grading device. The toad specification grading device comprises a conveying mechanism and grading mechanisms, the multiple grading mechanisms are located on the two sides of the conveying mechanism respectively, the conveying mechanism comprises a conveying belt, the conveying belt is of a structure in the prior art, a weight sensor is arranged in the conveying belt, and the weight sensor is controlled by an external computer. The grading mechanism comprises a collecting box, a bearing plate is arranged on one side of the collecting box, a motor is arranged on the bearing plate, a rotating shaft is arranged at the top end of the motor, the motor is rotationally connected with a scraping plate through the rotating shaft, and the motor is controlled by an external computer to be turned on and turned off. The toads do not need to be manually selected to be classified and graded, and the toad grading efficiency and the automation level are improved.

1. Name of the product in this design: Craft (Three-legged toad ornament). 2. Purpose of this design: decoration and display. 3. The key design elements of this product are the combination of shape, pattern, and color. 4. The image or photograph that best illustrates the design's key points: a 3D model. 5. The design for which protection is sought includes color.

This invention discloses a method for detecting toad oil, comprising: extracting oil samples from toad viscera using multi-stage temperature-controlled ultrasound and calculating the ultrasound power-time coupling efficiency coefficient; performing basic physicochemical tests on the oil samples to obtain physicochemical indicators; preparing test solutions and reference solutions; determining chromatographic detection conditions for chromatographic identification; calculating fingerprint similarity coefficients and linoleic acid content based on the chromatographic identification results; calculating characteristic peak consistency index based on the fingerprint similarity coefficients and linoleic acid content; calculating a comprehensive quality index based on the characteristic peak consistency index, physicochemical indicators, and ultrasound power-time coupling efficiency coefficient; and grading the toad oil quality based on the comprehensive quality index. This method constructs a complete data chain from sample pretreatment to final quality grading, achieving full-process traceability and standardized control of toad oil quality detection, significantly improving the systematic nature and reliability of the detection method.

This invention belongs to the field of biomedical technology, specifically relating to an ointment and its application. The toad oil used in this invention differs significantly in composition from toad venom, and its ointment is effective for various acute and chronic pain conditions. Animal experiments have confirmed that the toad oil ointment significantly reduces the frequency and delays the onset of acetic acid-induced writhing responses, demonstrating a clear analgesic effect. Clinical studies have shown that this ointment is rapidly effective and has a definite therapeutic effect on acute gout attacks, postherpetic neuralgia, and arthritis-related pain and swelling. The formulation effectively improves drug retention and user comfort, and no skin irritation has been observed. This development achieves comprehensive utilization of toad resources, providing an example for the sustainable development of traditional Chinese medicine and the efficient utilization of biological resources.

The invention relates to the technical field of artificial breeding of amphibians, in particular to an ecdysis promoting method for artificially imitating wild breeding of toads and application, according to the ecdysis promoting method, the toads are soaked in a soaking solution containing iodophor, the effective iodine concentration in the soaking solution is 0.1-0.2%, and the soaking time is 10-25 minutes. By the adoption of the ecdysis promoting method, the ecdysis period is greatly shortened, and compared with natural ecdysis (12-15 days), the ecdysis period is shortened to 5-7 days, and efficiency is greatly improved; and the toad cortex quality and yield can be obviously improved, and the toad cortex integrity reaches 92% or above.

The utility model discloses a multifunctional toad venom collector which comprises a support and a protective cover, the protective cover is fixedly connected with the support, the upper surface of the protective cover is fixedly connected with a mounting frame, one surface of the mounting frame is fixedly connected with a motor, the output end of the motor is fixedly connected with a screw rod, and the peripheral side face of the screw rod is provided with a moving block. The bottom face of the moving block is fixedly connected with an electric push rod, the output end of the electric push rod is fixedly connected with a connecting plate, a plurality of guide grooves are formed in the upper surface of the connecting plate, guide rods are installed in the guide grooves, springs are installed on the circumferential side faces of the guide rods, the bottom ends of the guide rods are fixedly connected with an extrusion soft plate, and a mesh plate is fixedly connected into the support. The toad collecting device solves the problems that in the prior art, toads are directly grabbed by hands and collected through a scraper, and the collecting method is prone to causing the fact that serous fluid is splashed into the eyes and harms the eyes.

The application discloses an external use delayed cream with natural ingredients and a preparation method thereof, belongs to the field of traditional Chinese medicine, and particularly relates to an external use delayed cream containing dry toad extract and toad oil and a preparation method thereof. The application is based on the local anesthetic properties of the dry toad extract and the toad oil, and an external use delayed cream is prepared by quantitatively controlling the dosages of the dry toad extract and the toad oil, which is used for treating male premature ejaculation and erectile dysfunction. The preparation has the advantages of direct action, sustained release and simple operation, and the preparation cost is low because the toad killed in breeding is used as a raw material. The application realizes the transformation of toad oil from waste to treasure, fully excavates the medicinal value of the toad oil, further perfects a reasonable utilization system of toad resources, and provides a practical scheme for efficient development of toad medicinal resources.

1. Name of the product in this design: Ornament (Treasure Pot Toad). 2. Purpose of this design: decoration, ornament. 3. The key design features of this product are the combination of shape and pattern. 4. The image or photograph that best illustrates the design's key points: a 3D model.

The application belongs to the field of traditional Chinese medicine, and particularly relates to a time-delay spray containing dry toad extract and toad oil and a preparation method thereof. The time-delay spray provided by the application can significantly treat male erectile dysfunction. The time-delay spray prepared by the application has the advantages of direct action, quantitative drug delivery, and no contact to avoid drug pollution. The application realizes the transformation of waste into treasure of toad oil, fully excavates the medicinal value of toad oil, further improves the rational utilization system of toad resources, and provides a practical scheme for efficient development of toad medicinal resources.

The utility model relates to the related technical field of toad tadpole breeding, in particular to a toad tadpole breeding pond which comprises a breeding pond body, an obliquely-arranged fixing frame is fixedly installed on one side of the breeding pond body, a side plate is welded to one side of the breeding pond body, and one end of the side plate is connected with a connecting pipe through a bearing. And one end of the connecting pipe is fixedly sleeved with a first chain wheel, a connecting rod is fixedly installed at one end of the connecting pipe, and a propeller is fixedly connected to the outer ring of one end of the connecting rod. According to the toad tadpole culture pond, water in the culture pond can be pushed to flow under the cooperation of a plurality of groups of connecting pipes, connecting rods and propellers, meanwhile, the oxygen content in water can be increased while the water flows under the cooperation of an oxygen supply mechanism, sufficient oxygen can promote metabolism of tadpoles, and therefore the toad tadpole culture pond can promote the metabolism of the tadpoles. Meanwhile, the flowing water environment can increase the activity amount of the tadpoles, the physique of the tadpoles is increased, the resistance of the tadpoles is improved, and the physiological change and adaptive adjustment of the tadpoles are facilitated.

The application belongs to the field of traditional Chinese medicine, and particularly relates to an external use delayed oil agent and a preparation method thereof. The dried skin of the Visternotus limnocharis which has scraped toad venom is combined with the male silkworm moth which has the functions of tonifying liver and kidney, strengthening yang and astringing essence to significantly improve male premature ejaculation and erectile dysfunction through water extraction and alcohol extraction. The male silkworm moth oil has unique promotion potential and development potential. The application combines the toad skin and the male silkworm moth oil to prepare the delayed oil agent, significantly improves male premature ejaculation and erectile dysfunction, and has the characteristics of direct action and simple operation. The preparation cost is low.

The application discloses a kind of Vibration Mountain beard toad hind leg / toe tissue cells and its quick acquisition method and application.The method comprises: the body surface disinfection of Vibration Mountain beard individual, cut and take hind leg or toe tissue, and put back after wound disinfection;After tissue is soaked in 75% alcohol and washed with PBS containing penicillin, streptomycin and amphotericin B, it is temporarily stored in 4 ℃ temporary storage solution;After tissue is cut, it is sequentially digested with trypsin and type I collagenase;In the optimized low permeability medium, primary culture is carried out at 25-27 ℃;When cell confluence reaches 60%-70%, it is passaged using trypsin-EDTA digestion;Freezing and recovery.The application realizes the non-lethal cell of Vibration Mountain beard for the first time Quick acquisition, cell first time migration time is only 3 days, grow into monolayer only 12-17 days, pollution rate is reduced to 5.6%, recovery survival rate reaches 76.19%, and provides efficient and reliable technical support for endangered amphibian germplasm resource preservation and biological research.

1. Name of the product in this design: Artificial Bait (Toad). 2. Purpose of this design: Fishing bait. 3. The key design feature of this product is its shape. 4. The image or photograph that best illustrates the key design points: Design 1 3D view. 5. The left view of Design 2 is symmetrical to the right view of Design 2, so the left view of Design 2 is omitted. 6. Design 1 is designated as the basic design.

1. Name of the designed product: cotton slippers (devil fish). 2. Use of the designed product: for daily wear. 3. Design points of the designed product: in shape. 4. Picture or photo best indicating the design points: perspective view.

The utility model relates to the related technical field of toad breeding, in particular to a vibration type integral feed feeding device for toad breeding, which comprises a base, a supporting rod is connected to the surface of the base, a bolt is connected to the surface of the base, and a discharging mechanism is arranged at one end of the supporting rod. According to the vibration type integral feed feeding device for feeding toads, through the arrangement of the discharging mechanism, when the vibration type integral feed feeding device is used, firstly, a user fills a feed box with a proper amount of feed, then when toads need to be fed, an electric push rod is remotely controlled to be started, and then one end of the electric push rod contracts and drives a partition plate to move until the partition plate moves into an empty groove; at the moment, the feed falls onto the surface of the mesh screen through the discharging pipe, so that the feed moves subsequently through the vibrating mechanism, and the toads are attracted to eat the feed.

The utility model discloses a toad breeding transfer box which comprises a transfer box body and a plurality of placing boxes, a mounting frame is connected in the transfer box body, a connecting block is installed in the mounting frame, a climbing frame, a mounting seat and a mounting plate are connected in the placing boxes, an extrusion block and a pull rod are installed in the mounting seat, and a first spring is installed on the peripheral side face of the pull rod. The extrusion block is connected with a connecting rod, the connecting rod is connected with a shielding net, a strip-shaped groove is formed in the upper surface of the shielding net, the mounting plate is connected with an elastic rod, the upper end of the elastic rod extends into the mounting base, and a second spring is mounted on the peripheral side face of the elastic rod. The toad transfer device solves the problems that according to an existing toad transfer device, generally, many toads are placed in a transfer box containing water, the transfer box is not layered, a large number of toads exist in the same space, oxygen supply is prone to being insufficient, if long-distance transfer is carried out, the survival rate of the toads is prone to being reduced, and the toads are prone to being damaged. Therefore, certain economic loss is caused.

The invention provides application of a venenum bufonis extract combined with beta-lactam antibiotics in medicines for treating MRSA infection, and belongs to the technical field of biological medicines. The invention proves that the active ingredients of the venenum bufonis have strong affinity and are stably combined with target proteins such as PBP2a, BlaZ and AgrA of MRSA, so that the antibacterial effect of antibiotics is enhanced. The venenum bufonis extract and beta-lactam antibiotics are combined for use, so that a remarkable synergistic effect is achieved, and the drug resistance of drug-resistant staphylococcus aureus is overcome. Mouse model experiments prove that the venenum bufonis and penicillin G combined treatment has a remarkable treatment effect on MRSA infected mice. The combined application of the venenum bufonis and the penicillin G can effectively control the MRSA infection, and a novel and effective treatment scheme is provided.

This invention provides a nutritional additive for improving the survival rate of artificially bred juvenile Toad tadpoles and its preparation method. This invention also provides a compound nutrient solution for improving the survival rate of artificially bred juvenile Toad tadpoles and its preparation method. This invention further provides the use of the above-mentioned nutritional additive and / or compound nutrient solution in improving the survival rate of artificially bred juvenile Toad tadpoles. This invention further provides a method for improving the survival rate of artificially bred juvenile Toad tadpoles. The method provided by this invention for improving the survival rate of artificially bred juvenile Toad tadpoles greatly increases the survival rate of juvenile Toad tadpoles weighing less than 5g, and is simple to operate, has low production costs, and a short growth cycle for the juveniles.

The invention discloses a traditional Chinese medicine compound multi-layer coated microsphere preparation for treating pancreatic cancer and a preparation method thereof. The preparation is prepared from the following active components in parts by weight: 10-35 parts of astragalus extract, 8-25 parts of ganoderma lucidum spore powder, 5-18 parts of sculellaria barbata general flavone and the like. The preparation method comprises the following steps: a) directionally extracting active ingredients; b) removing toxic substances from the dried toad skin through molecular imprinting; c) constructing three layers of coated microspheres, wherein a core layer is a nanocrystal (indirubin / chelidonine / toad peptide), a middle layer is a phospholipid complex (flavone / polysaccharide), and an outer layer is a pH response polysaccharide microsphere; and d) pressing to obtain the three-layer sustained-release tablet. The preparation realizes colon-targeted drug release, the bioavailability is obviously improved, the tumor inhibition rate of animal experiments is improved, and the chemotherapy drug resistance can be reversed.

This invention belongs to the field of traditional Chinese medicine, specifically relating to a delay towel containing dried toad extract and toad oil, and its preparation method. Based on the local anesthetic properties of dried toad extract and toad oil, this invention prepares a topical delay cream by quantitatively controlling the dosage of both, for the treatment of premature ejaculation and erectile dysfunction in men. The delay towel prepared by this invention has the advantages of direct action, individual packaging, immediate use, low cost, and minimal volatility of the active ingredients. This invention not only turns toad oil into a valuable resource and fully explores its medicinal value, but also further improves the rational utilization system of toad resources, providing a practical solution for the efficient development of toad medicinal resources.

The application provides PCR primers, detection kits and detection methods for identification of river toads. The PCR primers comprise Plectropomus primers and Lethrinus primers, the sequences of the upstream primer and the downstream primer of the Plectropomus primers are shown in SEQ ID NO. 1 and SEQ ID NO. 2 respectively, and the sequences of the upstream primer and the downstream primer of the Lethrinus primers are shown in SEQ ID NO. 3 and SEQ ID NO. 4 respectively. The application further provides a detection kit for identification of river toads, and also provides a corresponding detection method. The method of the application effectively realizes the detection of 18 Plectropomus and 4 Lethrinus river toad fishes, and does not have a detection reaction with other fishes, and fills the blank of the standard related to the identification of river toad fish species.