8 results about "Ecdysis" patented technology

According to the laodelphax striatellus ecdysis gene LsMH and the application thereof in biological prevention and control of laodelphax striatellus, the laodelphax striatellus ecdysis gene LsMH serves as one of key genes for ecdysis of laodelphax striatellus, the laodelphax striatellus ecdysis gene LsMH can be subjected to targeted interference, laodelphax striatellus can be remarkably inhibited from forming adults through eclosion, and laodelphax striatellus death is avoided, so that laodelphax striatellus population is the harm of the laodelphax striatellus to food crops is reduced. The control of laodelphax striatellus is carried out through a biological means, the method is safe and harmless to environment ecology and food, and a new strategy is provided for green control of laodelphax striatellus. The dsRNA or the interference reagent is prepared through transcription of the laodelphax striatellus ecdysis gene LsMH, the LsMH gene can be effectively interfered after the dsRNA or the interference reagent is injected into the laodelphax striatellus, the phenomena that wings are bent or incomplete, the abdomen is wizened and ecdysis is incomplete occur in the ecdysis process of laodelphax striatellus nymphs, laodelphax striatellus eclosion into adults is inhibited, laodelphax striatellus death is avoided, and biological control over laodelphax striatellus is achieved.

The invention discloses an intelligent barley pest breeding system, and relates to the technical field of barley pest breeding, and the system comprises an environment regulation and control module, an automatic feeding module, a growth monitoring module, an intelligent processing module and an early warning prompt module. A full-life-cycle data acquisition system covering an egg stage, a larva stage, a pupal stage and an adult stage is constructed, AI image analysis of a visual monitoring unit can accurately identify insect body length and body surface abnormity, a physiological monitoring unit tracks body weight change and body temperature fluctuation in real time, and a growth prediction model is combined to predict the growth of insects. According to the method, the incubation time and key growth nodes of an ecdysis window are accurately pre-judged, the strategy optimization unit dynamically adjusts environment parameters based on the growth stage, cross-dimension correlation analysis of breeding data is achieved, data support is provided for formulating a standardized breeding process, and the industry is promoted to be changed into intelligent management of data driving from experience driving.

The utility model discloses a multifunctional device for insect breeding and behavior testing. Relates to the technical field of insect breeding equipment. A gauze partition plate is arranged in the rearing cage; a liquid feeding device, a water feeding device, a solid feeding device, an adult oviposition container and a larva feeding box are placed in the feeding chamber; a pupation box is arranged in the pupation eclosion chamber; a foam layer floats on the surface of the liquid foodstuff and is lined with filter paper; a plurality of water feeding assemblies are arranged in the water feeder, and each water feeding assembly comprises moisturizing filter paper and a fiber cotton swab; the multiple water feeding assemblies abut against one another to fully seal the bottle opening of the water feeder. The device mainly solves the bottleneck problem of drowning of the adult fruit flies, provides a suitable oviposition container for an adult oviposition selection test, can well realize breeding, continuous breeding and adult trend and oviposition behavior selection research of the adult fruit flies, avoids a large amount of death of the tested adult fruit flies, saves manpower, material resources and financial resources, and improves the test efficiency of the adult fruit flies. And the artificial feeding reproduction and test efficiency is greatly improved.

The invention discloses dsRNA for preventing and controlling pest mites and application of the dsRNA, and belongs to the technical field of agricultural biology. According to a specific fusion gene segment obtained by comparative analysis of a plurality of pest mite FTZ-F1 genes, and by evaluating the application potential of the gene in pest mite prevention and control, the result shows that tetranychus urticae and tetranychus ilosi can die due to ecdysis failure by injecting artificially synthesized FTZ-F1 gene fusion dsRNA into the tetranychus, and the tetranychus urticae and tetranychus ilosi can die due to ecdysis failure of the artificially synthesized FTZ-F1 gene fusion dsRNA. An artificially synthesized FTZ-F1 gene fusion dsRNA is fed, off-target risk assessment is performed on the neoseiulus californicus which is the natural enemy of the spider mites, and the neoseiulus californicus is found to be capable of normally molting to enter an adult stage without influencing the survival of the natural enemy of the spider mites. The method can efficiently and accurately prevent and control pest mites, is high in pest killing efficiency and has a good application prospect.

The invention relates to the technical field of artificial breeding of amphibians, in particular to an ecdysis promoting method for artificially imitating wild breeding of toads and application, according to the ecdysis promoting method, the toads are soaked in a soaking solution containing iodophor, the effective iodine concentration in the soaking solution is 0.1-0.2%, and the soaking time is 10-25 minutes. By the adoption of the ecdysis promoting method, the ecdysis period is greatly shortened, and compared with natural ecdysis (12-15 days), the ecdysis period is shortened to 5-7 days, and efficiency is greatly improved; and the toad cortex quality and yield can be obviously improved, and the toad cortex integrity reaches 92% or above.

The invention belongs to the field of plant protection, and discloses application of an insect adult tray growth factor IDGF1 in prevention and control of bemisia tabaci. The bemisia tabaci is found to be incapable of normal ecdysis, slow in development and lethal after being interfered by the adult disc growth factor IDGF1. The pesticide has the double advantages of efficient killing and ecological safety, is easy to degrade when applied to the nature, has low residue and low harm to the natural environment, and is beneficial to green prevention and control of field pests.

The application provides a method for improving the ecdysis rate of Eriocheir sinensis, which can significantly improve the ecdysis rate of juvenile Eriocheir sinensis, reduce the mortality surge caused by ecdysis failure, and make the sustainable cultivation of subsequent larger size fry feasible. In addition, the method of the application also has the advantages of low cost and easy popularization.

The application belongs to the technical field of biology and relates to the prevention and treatment of Epilachna vigintioctopunctata, in particular to a dsRNA of an insect neuropeptide ecdysis-triggering hormone ETH gene and a preparation method and application thereof. The neuropeptide ETH gene of Epilachna vigintioctopunctata is screened through transcriptome sequencing; a fragment of the gene sequence with SEQ ID No. 2 is selected to synthesize dsRNA (dsETH). After 3-day-old larvae of Epilachna vigintioctopunctata are fed with dsETH (concentration >= 1 ng / μL), more than 70% of the Epilachna vigintioctopunctata are deformed and cannot feed on the 7th day, more than 70% of the Epilachna vigintioctopunctata die on the 10th day, and the deformation rates of 5, 10, 25 and 100 ng / μL are 85%, 90%, 95% and 100% respectively. The application provides a new prevention and treatment target for the prevention and treatment of Epilachna vigintioctopunctata based on RNA interference.