The invention discloses application of
nucleotide and fructo-
oligosaccharide, low-fish-
meal aquatic feed and a preparation method of the low-fish-
meal aquatic feed, and relates to the technical field of feed. The content of the
fish meal in the feed formula
system is only 15%, and the low-fish-
meal feed has the breeding effect equivalent to or even better than that of the traditional 35%
fish meal feed through reasonable matching and combination of
nucleotide and fructo-
oligosaccharide. The
nucleotide and the fructo-
oligosaccharide can synergistically regulate the expression of
appetite-related genes, optimize the intestinal micro-
ecological environment, improve the
digestive enzyme activity and activate multiple metabolic pathways, so that the food calling property and the
palatability of the low-fish-meal feed are remarkably improved, the feeding rate of the
eriocheir sinensis is increased, the growth is promoted, and the feed coefficient is reduced. Under the condition that a large amount of
plant protein replaces
fish meal, through the synergistic effect of nucleotide and fructo-oligosaccharide, the
appetite of the
eriocheir sinensis is not reduced, the growth is not slow, the
protein deposition rate of the feed is high, the cost of each
ton of the feed can be saved by about 1030 yuan, the reduction amplitude reaches 15.7%, and the economic requirements of large-scale culture of the
eriocheir sinensis are met.