14 results about "Eriocheir" patented technology

The invention discloses a method for culturing Eriocheir sinensis with oyster shells, which comprises the following steps: S1, pretreating a crab pond, and laying a microporous oxygenation pipeline; s2, collecting the waste oyster shells, and performing cleaning, disinfection and drying treatment; s3, the oyster shells treated in the step S2 are stacked in a long ridge shape in the crab pond in the direction parallel to the micropore oxygenation pipeline, and the whole crab pond is disinfected; s4, waterweed is planted in the oyster shell ridge direction in the middle ten days of January; s5, crab species are stocked in early February, so that the environment richness of the crab pond is improved, self-killing of the river crabs is reduced, the survival rate is increased, and the adult crab breeding technology is more environmentally friendly and efficient.

The invention relates to the technical field of aquaculture, in particular to a method for improving the growth performance and nutritional quality of female eriocheir sinensis. The method comprises the following steps: pretreating phaeodactylum tricornutum to obtain pretreated phaeodactylum tricornutum; in the feeding process of female eriocheir sinensis, basic feed is fed into a feeding water body, and the pretreated phaeodactylum tricornutum is additionally fed; wherein the feeding amount of the pretreated phaeodactylum tricornutum is dynamically adjusted according to the change of the feeding water temperature. By adding the phaeodactylum tricornutum, the food source of the eriocheir sinensis is directly changed, and the final weight, gonad index, meat percentage, total edible rate and fatness of female crabs are remarkably improved; protein, amino acid and vitamins which are rich in phaeodactylum tricornutum can directly optimize the growth and quality of the eriocheir sinensis, and especially under the condition of high-density culture, the quality of the eriocheir sinensis is correspondingly improved while the growth performance of the eriocheir sinensis can be remarkably improved.

The invention relates to a male and female specific DNA (deoxyribonucleic acid) sequence for identifying genetic sex of eriocheir sinensis and application of the male and female specific DNA sequence. The nucleotide sequence of the male and female specific DNA sequence is shown as SEQ ID NO: 1; the sex identification method is realized by identifying the male and female specific DNA sequence, and comprises the following steps: extracting genome DNA of the eriocheir sinensis; the method comprises the following steps: carrying out PCR (Polymerase Chain Reaction) amplification by taking genome DNA as a template, wherein primer sequences for PCR amplification are shown as SEQ ID NO: 2 and SEQ ID NO: 3; agarose electrophoresis detection is carried out on amplification products, the products with male and female specific DNA sequence amplification bands are female eriocheir sinensis, and otherwise, the products are male eriocheir sinensis. The primer pair developed and designed based on the male and female specific DNA sequence is proved to have high accuracy and reliability in early sex identification of the eriocheir sinensis, and important technical support is provided for sex control breeding, unisexual breeding and genetic improvement of the eriocheir sinensis.

The present application relates to the technical field of RNA interference, and particularly discloses a RNA interference fragment of a crustacean hyperglycemic hormone gene and application thereof. The RNA interference fragment is injected into the third step of a river crab in a manner of injection, and is used for interfering with mRNA expression of a crustacean hyperglycemic hormone gene CHH of the river crab. The fighting behavior of Eriocheir sinensis is effectively controlled, which is beneficial to the yield improvement of large-scale breeding of Eriocheir sinensis and reduction of economic losses caused by the incomplete Eriocheir sinensis due to fighting.

The utility model discloses a sealed eriocheir sinensis tissue sample collection box, apply in sample collection technical field, including box, the rear side of box is connected with the apron through the axle pin swing, the inner chamber fixed mounting of box has the deposit storehouse, the both sides of box front side all are connected with the clamping component through the axle pin swing, the clamping component includes the clamping plate, the rear side of clamping plate is fixed with apron mounting, the both sides of box and apron front side and rear side all are clamped with supplementary sealing component. When needing to increase the sealing property of the collection box sealed through the clamping component, the user unfolds the supplementary sealing component at this moment, the positioning shell in supplementary sealing component is contacted with the box, and is fixed after through bolt and box, then clamps the positioning plate and apron, fixes supplementary sealing component between box and apron through the resilience of limiting spring, to increase the sealing property of the collection box sealed through the clamping component.

The invention discloses a construction method and application of an Eriocheir sinensis gut-brain axis research model, according to the method, metronidazole, neomycin and vancomycin are added to commercial feed according to the mass ratio of 10: 10: 5 g / kg to prepare compound antibiotic feed, and the Eriocheir sinensis is continuously fed for 96 h. According to the method, a standardized research model capable of synchronously and controllably presenting the causal relation of'intestinal flora disturbance-nervous system response 'is constructed in crustacean for the first time. The model is short in construction period, high in stability and high in repeatability, and a key research tool is provided for deeply analyzing the enteric-cerebral axis interaction mechanism of the eriocheir sinensis and even crustacean, screening neural active substances and evaluating neurotoxicity of environmental stress.

The present application belongs to the technical field of biological information analysis, and particularly relates to a microsatellite marker of a temperature regulation related functional gene of Scylla paramamosain, a detection method, a kit, an analysis method, a screening method and a breeding method. Through transcriptome data analysis, the present application screens a temperature adaptability related functional gene, develops a microsatellite marker of the temperature regulation related functional gene of Scylla paramamosain, and provides polymorphic primers, establishes a technical system and a detection method for development of the microsatellite marker of the functional gene of Scylla paramamosain. The present application not only lays a foundation for Scylla paramamosain genetic diversity research, genetic map construction and evolution analysis, but also provides valuable and reliable molecular markers for breeding of fine varieties of Scylla paramamosain.

The utility model is suitable for the technical field of Eriocheir sinensis culture, and provides a Eriocheir sinensis storage and culture pool, which comprises: a storage and culture pool body for culturing Eriocheir sinensis, wherein the storage and culture pool body comprises a plurality of culture pools, and the plurality of culture pools are used for placing and culturing Eriocheir sinensis; a mounting frame fixedly mounted on the storage and culture pool body; a feeding box assembled on the mounting frame, wherein the feeding box is used for placing food for feeding Eriocheir sinensis; and a feeding mechanism assembled on the feeding box, wherein the feeding mechanism is used for feeding the food placed in the feeding box into the storage and culture pool body. The Eriocheir sinensis storage and culture pool can culture Eriocheir sinensis, and can automatically feed the cultured Eriocheir sinensis, thereby reducing the labor burden of the staff and improving the culture efficiency.

The invention discloses a construction method and application of an Eriocheir sinensis intestinal flora deletion model, the method comprises the following steps: feeding Eriocheir sinensis with a feed mixed with mixed antibiotics for 96 hours to obtain the Eriocheir sinensis intestinal flora deletion model; the mixed antibiotic is prepared from metronidazole, neomycin and vancomycin. A plate culture method verifies that the optimized core antibiotic concentration group can enable the clearance rate of intestinal content florae to reach 95% or above and the clearance rate of fecal florae to reach 90% or above. According to the method, the intestinal flora deletion model of the eriocheir sinensis is constructed for the first time, the method has the advantages of being high in removal efficiency, good in repeatability, small in stress and the like, the flora is restorable after drug administration is stopped, and a standardized experimental tool is provided for studying the intestinal-brain axis mechanism, physiological metabolism and healthy breeding of crustacean.

The invention relates to the technical field of Chinese mitten crab seed cultivation, in particular to a method for increasing the survival rate of Chinese mitten crab seed early cultivation, which comprises three key steps of pond design, equipment arrangement and juvenile crab cultivation. Pond structural design is optimized, special breeding equipment is configured, and staged refined breeding management is implemented, so that the growth requirements of the fries are accurately matched with the environment and bait; planting blocks and bait and fertilizer blocks are arranged at intervals in the pond, aquatic plant cultivation and water body pretreatment are matched, the equipment comprises a lamp belt with a bait gathering function and manual and automatic double feeders matched with different breeding stages, and bait types and feeding modes are switched according to the seedling development stage in the breeding process; the method effectively solves the survival adaptation problem of the offspring seeds in the metamorphosis shelling period and the feeding habit conversion period, is suitable for large-scale early-stage cultivation of the eriocheir sinensis offspring seeds, and has good industrial application value and popularization prospects.

The present application relates to a kind of based on side scan sonar image's Eriocheir sinensis quantity and size evaluation system and equipment, belong to aquaculture monitoring, underwater acoustic detection and image intelligent identification technical field, the system includes side scan sonar collection module, data preprocessing module, bright spot map patch detection module, Eriocheir sinensis candidate target screening module, bright spot-shading identification module, multiple track deduplication module, resource quantity evaluation and visualization module.The method realizes Eriocheir sinensis individual level automatic identification, the assessment of Eriocheir sinensis quantity, size distribution and total resource quantity in pond.Make Eriocheir sinensis breeding pond can not interfere with production, quickly and accurately obtain Eriocheir sinensis quantity and specification structure information in pond, provide data support for feeding management, disease early warning, fishing decision etc..

The application provides a method for improving the ecdysis rate of Eriocheir sinensis, which can significantly improve the ecdysis rate of juvenile Eriocheir sinensis, reduce the mortality surge caused by ecdysis failure, and make the sustainable cultivation of subsequent larger size fry feasible. In addition, the method of the application also has the advantages of low cost and easy popularization.

This invention discloses the development and application of a specific marker associated with the shell thickness trait of the Chinese mitten crab (Eriocheir sinensis). This molecular marker is located in Chr09 of the Chinese mitten crab genome. Esine02654 The gene region exhibits significant sequence length polymorphism: the wild type contains a 72 bp nucleotide sequence, while in the mutant, this sequence is mutated to a single base A or deleted. This invention utilizes the specific design of downstream primers located within this variant region to develop a primer pair for detecting this structural variation. Amplification using this primer pair shows a specific 109 bp band in thick-shelled individuals (wild-type), while thin-shelled individuals (homozygous deletion mutant) show no amplification band due to the absence of the primer binding site. This marker detection is simple, and the results are intuitively interpreted, showing either a band or no band, making it suitable for early-stage auxiliary screening and breeding of the thick-shell trait in Chinese mitten crabs.

The application discloses a method for breeding a new germplasm of Eriocheir sinensis in cold regions, and belongs to the technical field of aquaculture. The method comprises the following steps: screening of two-year-old immature Eriocheir sinensis; overwintering of the two-year-old immature Eriocheir sinensis; water body disinfection, overwintering density, feed feeding and water body indexes; breeding of three-year-old large-size Eriocheir sinensis: breeding pond and aquatic plant planting; seedling release; management during the breeding period; and the three-year-old Eriocheir sinensis is sold as a commodity crab when the size and gonadal development meet the requirements. In the application, a new Eriocheir sinensis germplasm, i.e., three-year-old Eriocheir sinensis, is bred, and the size of the Eriocheir sinensis bred in cold regions is improved. In the application, the average size of the female three-year-old Eriocheir sinensis bred from two-year-old immature Eriocheir sinensis is (96.82+2.48) g, and the average size of the male three-year-old Eriocheir sinensis is (135.91+4.20) g. Compared with the two-year-old mature Eriocheir sinensis bred by using the prior art, the size is significantly increased, and the purpose of breeding large-size Eriocheir sinensis is achieved.