23 results about "Eriocheir" patented technology

The application discloses bacillus subtilis G190 and application thereof in Eriocheir sinensis breeding. After soybean meal is fermented by the bacillus subtilis G190, aquatic product feed is prepared, and the Eriocheir sinensis is bred by using the feed, so that the survival rate and flavor substances of the Eriocheir sinensis can be remarkably improved. The effective implementation of the research can provide scientific theoretical basis for the effect, regulation mechanism and popularization and application of the probiotic bacillus feed, and effectively serve further development of sustainable ecological breeding technology of the Eriocheir sinensis.

The invention discloses a method for culturing Eriocheir sinensis with oyster shells, which comprises the following steps: S1, pretreating a crab pond, and laying a microporous oxygenation pipeline; s2, collecting the waste oyster shells, and performing cleaning, disinfection and drying treatment; s3, the oyster shells treated in the step S2 are stacked in a long ridge shape in the crab pond in the direction parallel to the micropore oxygenation pipeline, and the whole crab pond is disinfected; s4, waterweed is planted in the oyster shell ridge direction in the middle ten days of January; s5, crab species are stocked in early February, so that the environment richness of the crab pond is improved, self-killing of the river crabs is reduced, the survival rate is increased, and the adult crab breeding technology is more environmentally friendly and efficient.

The invention relates to the technical field of aquaculture, in particular to a method for improving the growth performance and nutritional quality of female eriocheir sinensis. The method comprises the following steps: pretreating phaeodactylum tricornutum to obtain pretreated phaeodactylum tricornutum; in the feeding process of female eriocheir sinensis, basic feed is fed into a feeding water body, and the pretreated phaeodactylum tricornutum is additionally fed; wherein the feeding amount of the pretreated phaeodactylum tricornutum is dynamically adjusted according to the change of the feeding water temperature. By adding the phaeodactylum tricornutum, the food source of the eriocheir sinensis is directly changed, and the final weight, gonad index, meat percentage, total edible rate and fatness of female crabs are remarkably improved; protein, amino acid and vitamins which are rich in phaeodactylum tricornutum can directly optimize the growth and quality of the eriocheir sinensis, and especially under the condition of high-density culture, the quality of the eriocheir sinensis is correspondingly improved while the growth performance of the eriocheir sinensis can be remarkably improved.

The invention relates to a male and female specific DNA (deoxyribonucleic acid) sequence for identifying genetic sex of eriocheir sinensis and application of the male and female specific DNA sequence. The nucleotide sequence of the male and female specific DNA sequence is shown as SEQ ID NO: 1; the sex identification method is realized by identifying the male and female specific DNA sequence, and comprises the following steps: extracting genome DNA of the eriocheir sinensis; the method comprises the following steps: carrying out PCR (Polymerase Chain Reaction) amplification by taking genome DNA as a template, wherein primer sequences for PCR amplification are shown as SEQ ID NO: 2 and SEQ ID NO: 3; agarose electrophoresis detection is carried out on amplification products, the products with male and female specific DNA sequence amplification bands are female eriocheir sinensis, and otherwise, the products are male eriocheir sinensis. The primer pair developed and designed based on the male and female specific DNA sequence is proved to have high accuracy and reliability in early sex identification of the eriocheir sinensis, and important technical support is provided for sex control breeding, unisexual breeding and genetic improvement of the eriocheir sinensis.

The invention provides a primer probe group, a kit and a detection method for detecting microsporidia of eriocheir sinensis, and belongs to the technical field of pathogen detection. The invention provides a primer probe group for detecting microsporidia of eriocheir sinensis. The primer probe group comprises a primer pair and a TaqMan probe, the nucleotide sequences of the primer pair and the TaqMan probe are as shown in SEQ ID NO: 1 to SEQ ID NO: 3. In the invention, the primer probe group can specifically detect the eriocheir sinensis microsporidia, and does not have cross reaction with aeromonas veronii, vibrio parahaemolyticus, eriocheir sinensis spiroplasma, white spot syndrome virus and siniperca chuatsi iridovirus. The lowest detection limit of the primer probe group is 4.6 * 10 < 1 > copies / mu L, the sensitivity is improved by 1000 times compared with that of common PCR, and great detection advantages are shown.

The invention provides paste slurry type pellet feed for eriocheir sinensis and a production method and device of the paste slurry type pellet feed. According to the invention, compounded cuttlefish paste, shrimp paste and enzymolysis fish paste form external spraying paste, base material particles are put into a rotating cylinder to form dynamic rolling, and the external spraying paste is sprayed to the surfaces of the rolling base material particles through a nozzle of a spraying pipe, so that the external spraying paste uniformly coats the base material particles and is prevented from caking and falling off; finally, collaborative optimization of food calling enhancement and structural stability is achieved, and the technical defects that traditional pellet feed is insufficient in food calling, poor in water resistance and low in production efficiency are systematically overcome, so that the pellet feed has the chilled fish-grade food calling performance and the stability of commercial feed; an industrial feed solution with efficient ingestion promotion and lasting water resistance is provided for sustainable development of large-scale culture of the eriocheir sinensis.

The present application relates to the technical field of RNA interference, and particularly discloses a RNA interference fragment of a crustacean hyperglycemic hormone gene and application thereof. The RNA interference fragment is injected into the third step of a river crab in a manner of injection, and is used for interfering with mRNA expression of a crustacean hyperglycemic hormone gene CHH of the river crab. The fighting behavior of Eriocheir sinensis is effectively controlled, which is beneficial to the yield improvement of large-scale breeding of Eriocheir sinensis and reduction of economic losses caused by the incomplete Eriocheir sinensis due to fighting.

The utility model discloses a sealed eriocheir sinensis tissue sample collection box, apply in sample collection technical field, including box, the rear side of box is connected with the apron through the axle pin swing, the inner chamber fixed mounting of box has the deposit storehouse, the both sides of box front side all are connected with the clamping component through the axle pin swing, the clamping component includes the clamping plate, the rear side of clamping plate is fixed with apron mounting, the both sides of box and apron front side and rear side all are clamped with supplementary sealing component. When needing to increase the sealing property of the collection box sealed through the clamping component, the user unfolds the supplementary sealing component at this moment, the positioning shell in supplementary sealing component is contacted with the box, and is fixed after through bolt and box, then clamps the positioning plate and apron, fixes supplementary sealing component between box and apron through the resilience of limiting spring, to increase the sealing property of the collection box sealed through the clamping component.

The invention discloses a construction method and application of an Eriocheir sinensis gut-brain axis research model, according to the method, metronidazole, neomycin and vancomycin are added to commercial feed according to the mass ratio of 10: 10: 5 g / kg to prepare compound antibiotic feed, and the Eriocheir sinensis is continuously fed for 96 h. According to the method, a standardized research model capable of synchronously and controllably presenting the causal relation of'intestinal flora disturbance-nervous system response 'is constructed in crustacean for the first time. The model is short in construction period, high in stability and high in repeatability, and a key research tool is provided for deeply analyzing the enteric-cerebral axis interaction mechanism of the eriocheir sinensis and even crustacean, screening neural active substances and evaluating neurotoxicity of environmental stress.

The present application belongs to the technical field of biological information analysis, and particularly relates to a microsatellite marker of a temperature regulation related functional gene of Scylla paramamosain, a detection method, a kit, an analysis method, a screening method and a breeding method. Through transcriptome data analysis, the present application screens a temperature adaptability related functional gene, develops a microsatellite marker of the temperature regulation related functional gene of Scylla paramamosain, and provides polymorphic primers, establishes a technical system and a detection method for development of the microsatellite marker of the functional gene of Scylla paramamosain. The present application not only lays a foundation for Scylla paramamosain genetic diversity research, genetic map construction and evolution analysis, but also provides valuable and reliable molecular markers for breeding of fine varieties of Scylla paramamosain.

The application discloses a siRNA targeting for Eriocheir sinensis RDH11 gene and application thereof, and belongs to the technical field of molecular biology of aquaculture. The siRNA is siRDH11 targeting for the Eriocheir sinensis RDH11 gene, and a sequence is shown as SEQ ID NO: 1 or 2; the siRNA is used for preparing a drug for inhibiting fighting behavior of the Eriocheir sinensis, and the siRNA is used as an active ingredient; in particular, the drug is injected into the Eriocheir sinensis in an injection mode, and the expression amount of the RDH11 gene in a brain ganglion or a thoracic ganglion of the Eriocheir sinensis is inhibited or reduced through the siRNA. The siRNA targeting for the Eriocheir sinensis RDH11 gene is developed to realize molecular precise regulation of fighting behavior of the Eriocheir sinensis, and the problem that the aquaculture yield is reduced due to the fighting behavior of the Eriocheir sinensis in the aquaculture process is solved, and the application has important industrial value.

The invention discloses a feed for improving the quality of eriocheir sinensis and a preparation method of the feed, and belongs to the technical field of crab feeds. The feed comprises the following raw materials in parts by weight: 30-40 parts of fish meal, 5-15 parts of chicken meal, 8-12 parts of soybean meal, 4-6 parts of peanut meal, 2-5 parts of egg white powder, 1-5 parts of pig protein powder, 1-5 parts of sesame powder, 15-20 parts of starch, 5-8 parts of soybean oil, 0.5-1 part of 50% choline chloride, 0.5-1.5 parts of monocalcium phosphate, 0.5-1 part of vitamin mineral premix and 1-3 parts of zeolite powder. The crab feed obtained by the invention can obviously improve the quality of the eriocheir sinensis.

The utility model is suitable for the technical field of Eriocheir sinensis culture, and provides a Eriocheir sinensis storage and culture pool, which comprises: a storage and culture pool body for culturing Eriocheir sinensis, wherein the storage and culture pool body comprises a plurality of culture pools, and the plurality of culture pools are used for placing and culturing Eriocheir sinensis; a mounting frame fixedly mounted on the storage and culture pool body; a feeding box assembled on the mounting frame, wherein the feeding box is used for placing food for feeding Eriocheir sinensis; and a feeding mechanism assembled on the feeding box, wherein the feeding mechanism is used for feeding the food placed in the feeding box into the storage and culture pool body. The Eriocheir sinensis storage and culture pool can culture Eriocheir sinensis, and can automatically feed the cultured Eriocheir sinensis, thereby reducing the labor burden of the staff and improving the culture efficiency.

The invention discloses a construction method and application of an Eriocheir sinensis intestinal flora deletion model, the method comprises the following steps: feeding Eriocheir sinensis with a feed mixed with mixed antibiotics for 96 hours to obtain the Eriocheir sinensis intestinal flora deletion model; the mixed antibiotic is prepared from metronidazole, neomycin and vancomycin. A plate culture method verifies that the optimized core antibiotic concentration group can enable the clearance rate of intestinal content florae to reach 95% or above and the clearance rate of fecal florae to reach 90% or above. According to the method, the intestinal flora deletion model of the eriocheir sinensis is constructed for the first time, the method has the advantages of being high in removal efficiency, good in repeatability, small in stress and the like, the flora is restorable after drug administration is stopped, and a standardized experimental tool is provided for studying the intestinal-brain axis mechanism, physiological metabolism and healthy breeding of crustacean.

The invention relates to the technical field of Chinese mitten crab seed cultivation, in particular to a method for increasing the survival rate of Chinese mitten crab seed early cultivation, which comprises three key steps of pond design, equipment arrangement and juvenile crab cultivation. Pond structural design is optimized, special breeding equipment is configured, and staged refined breeding management is implemented, so that the growth requirements of the fries are accurately matched with the environment and bait; planting blocks and bait and fertilizer blocks are arranged at intervals in the pond, aquatic plant cultivation and water body pretreatment are matched, the equipment comprises a lamp belt with a bait gathering function and manual and automatic double feeders matched with different breeding stages, and bait types and feeding modes are switched according to the seedling development stage in the breeding process; the method effectively solves the survival adaptation problem of the offspring seeds in the metamorphosis shelling period and the feeding habit conversion period, is suitable for large-scale early-stage cultivation of the eriocheir sinensis offspring seeds, and has good industrial application value and popularization prospects.

The invention relates to a method for replacing cholesterol in Eriocheir sinensis feed with modified phytosterol. According to the method, 0.045%-0.064% by mass of microcapsule-coated modified phytosterol is added into the eriocheir sinensis compound feed to replace part or all of cholesterol. The main components of the modified phytosterol comprise beta-sitosterol, stigmasterol, campesterol and campesterol, and the modified phytosterol has high water solubility and bioavailability. The feed is used for feeding the eriocheir sinensis in a growing period, a fattening period or an early breeding period after being extruded and granulated. Application results show that the method can effectively improve the gonad index, the estradiol content and the reproductive performance of the eriocheir sinensis and optimize the feed cost structure. The method is easy and convenient to operate, raw materials are easy to obtain, and the method is suitable for the fields of cholesterol replacement and functional feed development in aquaculture.

The invention discloses a molecular marker related to the body weight character of eriocheir sinensis and application of the molecular marker, and relates to the technical field of biology. The nucleotide sequence of the molecular marker is as shown in SEQ ID NO.3; an SNP (Single Nucleotide Polymorphism) site exists at the 141 basic group of the molecular marker and is G / A mutation. According to the invention, SNP loci related to the body weight character of the eriocheir sinensis are analyzed through genome-wide association analysis, and candidate SNP loci, namely Chr1616673106Ggt, which are obviously related to the body weight character are screened; a. A result shows that the gene is Chr1616673106Ggt; in A, the GA genotype is a preferred genotype. The excellent body weight character of the individual with the genotype is more prominent. Therefore, the SNP site can be used for early breeding of the eriocheir sinensis with excellent growth traits, and the accuracy is high. The molecular marker provided by the invention has an application prospect in Eriocheir sinensis body weight molecular marker-assisted breeding.

The invention discloses a breeding method for high-temperature-resistant germplasm of eriocheir sinensis, and relates to the technical field of biology. The method comprises the following steps: performing gene detection on an F0-generation breed conservation breeding group of the eriocheir sinensis, and selecting individuals with high-temperature-resistant main effect genotypes to obtain an F0-generation high-temperature-resistant breeding group; carrying out self-propagation on the F0-generation high-temperature-resistant breeding population to obtain an F1-generation breeding population; performing gene detection on the F1-generation breeding population, and selecting individuals with high-temperature-resistant main effect genotypes to obtain an F1-generation high-temperature-resistant breeding population; carrying out self-propagation on the F1-generation high-temperature-resistant breeding population to obtain an F2-generation breeding population; performing gene detection on the F2-generation breeding population, and selecting individuals with high-temperature-resistant main effect genotypes to obtain an F2-generation high-temperature-resistant breeding population; and carrying out self-propagation on the F2-generation high-temperature-resistant breeding population to obtain an F3-generation high-temperature-resistant breeding population. According to the method provided by the invention, the eriocheir sinensis with high temperature resistance can be efficiently obtained.

The present application relates to a kind of based on side scan sonar image's Eriocheir sinensis quantity and size evaluation system and equipment, belong to aquaculture monitoring, underwater acoustic detection and image intelligent identification technical field, the system includes side scan sonar collection module, data preprocessing module, bright spot map patch detection module, Eriocheir sinensis candidate target screening module, bright spot-shading identification module, multiple track deduplication module, resource quantity evaluation and visualization module.The method realizes Eriocheir sinensis individual level automatic identification, the assessment of Eriocheir sinensis quantity, size distribution and total resource quantity in pond.Make Eriocheir sinensis breeding pond can not interfere with production, quickly and accurately obtain Eriocheir sinensis quantity and specification structure information in pond, provide data support for feeding management, disease early warning, fishing decision etc..

The invention relates to the technical field of aquatic product tracing methods, in particular to a stable isotope-based geographical source tracing method and system for eriocheir sinensis. By comprehensively adopting stable isotope data of multiple tissues such as muscle, hepatopancreas and shells of the eriocheir sinensis, a five-dimensional feature vector containing tissue type information is constructed, the limitation that a traditional method depends on a single tissue is broken through, and collaborative analysis of isotope features of multiple tissues remarkably enhances the discrimination ability of the model, so that the model is more accurate and reliable. The method comprises the following steps of: firstly, selecting a model for a long-term breeding scene, so that the classification accuracy of the model in the long-term breeding scene is improved to 97-98%, eliminating dimensional difference through Z-score standardization by using a hybrid modeling strategy of partial least squares discriminant analysis (PLSDA) and logistic regression (LR), extracting potential variables by using the PLSDA to capture a nonlinear relationship among multiple isotopes, and then realizing high-robustness classification through the LR; the technical problem that in the prior art, it is difficult to recognize the temporarily-bred eriocheir sinensis is solved.

The application provides a method for improving the ecdysis rate of Eriocheir sinensis, which can significantly improve the ecdysis rate of juvenile Eriocheir sinensis, reduce the mortality surge caused by ecdysis failure, and make the sustainable cultivation of subsequent larger size fry feasible. In addition, the method of the application also has the advantages of low cost and easy popularization.

This invention discloses the development and application of a specific marker associated with the shell thickness trait of the Chinese mitten crab (Eriocheir sinensis). This molecular marker is located in Chr09 of the Chinese mitten crab genome. Esine02654 The gene region exhibits significant sequence length polymorphism: the wild type contains a 72 bp nucleotide sequence, while in the mutant, this sequence is mutated to a single base A or deleted. This invention utilizes the specific design of downstream primers located within this variant region to develop a primer pair for detecting this structural variation. Amplification using this primer pair shows a specific 109 bp band in thick-shelled individuals (wild-type), while thin-shelled individuals (homozygous deletion mutant) show no amplification band due to the absence of the primer binding site. This marker detection is simple, and the results are intuitively interpreted, showing either a band or no band, making it suitable for early-stage auxiliary screening and breeding of the thick-shell trait in Chinese mitten crabs.

The application discloses a method for breeding a new germplasm of Eriocheir sinensis in cold regions, and belongs to the technical field of aquaculture. The method comprises the following steps: screening of two-year-old immature Eriocheir sinensis; overwintering of the two-year-old immature Eriocheir sinensis; water body disinfection, overwintering density, feed feeding and water body indexes; breeding of three-year-old large-size Eriocheir sinensis: breeding pond and aquatic plant planting; seedling release; management during the breeding period; and the three-year-old Eriocheir sinensis is sold as a commodity crab when the size and gonadal development meet the requirements. In the application, a new Eriocheir sinensis germplasm, i.e., three-year-old Eriocheir sinensis, is bred, and the size of the Eriocheir sinensis bred in cold regions is improved. In the application, the average size of the female three-year-old Eriocheir sinensis bred from two-year-old immature Eriocheir sinensis is (96.82+2.48) g, and the average size of the male three-year-old Eriocheir sinensis is (135.91+4.20) g. Compared with the two-year-old mature Eriocheir sinensis bred by using the prior art, the size is significantly increased, and the purpose of breeding large-size Eriocheir sinensis is achieved.