154 results about "Eriocheir" patented technology

The invention discloses a Yangtze river system eriocheir sinensis compound feed, which is prepared from the following materials: fish meal, bean pulp, cottonseed meal, rapeseed meal, peanut meal, corn germ meal, cuttlefish paste, shrimp meal, soybean phosphatide powder, soya-bean oil, fish oil, lard oil, monocalcium phosphate, high protein flour, shell pigment, trace elements for crab, complex vitamins for crab, choline chloride, a binder, zeolite powder, bacillus licheniformis premix, and astragalus polysaccharide premix. The preparation method of the Yangtze river system eriocheir sinensis compound feed comprises the steps of: mixing materials twice, evenly mixing, pelletizing, keeping warm, cooling, sieving, packaging and the like. The Yangtze river system eriocheir sinensis compound feed can enhance the disease resistance of the eriocheir sinensis, quickens the growth speed of the eriocheir sinensis, enriches crab roe and crab cream, improves the nutritive value of the eriocheir sinensis, increases production and improves productivity for a farmer. The digestion and absorption efficiency of the eriocheir sinensis on the materials can be effectively strengthened, and the stability of particles in water is strong.

The invention discloses a special formula feed for raising cream of eriocheir sinensis and a preparation method of the special formula feed. The special formula feed for raising cream of the eriocheir sinensis is prepared from the following components in parts by weight: 10-20 parts of fish meal, 5-10 parts of hydrolysis fish protein, 2-5 parts of beer yeast, 2-5 parts of sleeve-fish cream, 2-5 parts of euphausiid powder, 5-10 parts of shrimp shell powder, 15-25 parts of soybean meal, 5-10 parts of corn protein powder, 15-20 parts of flour, 1-3 parts of monocalcium phosphate, 0.1-0.5 part of multidimensional premix, 0.5-1 part of multi-mineral premix, 0.1-0.5 part of choline chloride, 0.2-0.5 part of salt, 1-3 parts of soybean oil, 1.5-3.5 parts of fish oil, 0.01-0.05 part of an antioxidant, 0.1-0.5 part of a mould inhibitor, 2-5 parts of bentonite, 0.1-0.5 part of coated lysine, 1-4 parts of gulfweed and 0.1-0.5 part of yolk powder. The special formula feed can satisfy the needs of the eriocheir sinensis in growth and cream raising and is capable of improving the feed attraction of the feed and the quality of commodity crabs.

The invention discloses an ecological breeding method of Eriocheir sinensis. The method comprises the steps that a pond is prepared firstly, an annular groove, an anti-permeable membrane and an inlet and outlet water pipe with a filtering mesh are arranged, water is poured after exposure, water plants are planted and loaches and diatom are placed in the pond after disinfection,, a biological agent and chicken manure are scattered and fertilized after water adding, the temperature, the dissolved oxygen and the PH value of the water are adjusted, the breeding pond is obtained, young Eriocheir sinensis is thrown into the breeding pond after being sterilized, feeding is carried out according to different growth periods of the Eriocheir sinensis, plant ash, the biological agent and inorganic salt type nutrient substances are scattered and fertilized regularly, and periodic water cleaning and disinfecting are carried out. According to the ecological breeding method, the environment and nutrient substances needed in the growth period of the Eriocheir sinensis can be provided, fast growth of the Eriocheir sinensis can be promoted, the specification and yield of the Eriocheir sinensis can be improved, and the method is ecological, environmentally friendly and economical.

The present invention discloses an eriocheir sinensis compound feed, which comprises the following components: fish meal, soybean meal, soy protein powder, brewer's yeast powder, squid paste, shrimp bran, rice bran, flour, fish oil, a shrimp and crab premix, betaine, choline, and calcium dihydrogen phosphate, wherein a weight ratio of the components is sequentially 18-22:13-17:10-14:4-6:4-6:5-7:4-6:5-7:18-22:0.8-1.2:1.5-2.5:0.3-0.7:0.3-0.7:1.8-2.2. According to the present invention, microbial agents and health drugs are adopted so as to substantially improve anti-stress ability and disease prevention of aquatic products, and guarantee is provided for achievements of the maximum growth amount, the best economic benefits, and green pollution-free aquatic products.

The invention relates to a directional mating and breeding method in Eriocheir sinensis breeding, comprising the following steps of: referring to the Eriocheir sinensis standard GB/T19783-2005, selecting male and female Eriocheir sinensis individuals with mature gonads from different species groups in the first ten-day period of November as parents, after labeling, breeding the male and female Eriocheir sinensis individuals separately, and strengthening cultivation; in the last ten-day period of November, pairing and putting the parents subjected to cultivation strengthening into cylindrical net cages weaved by polyethylene soft plastic, wherein each cylindrical net cage is 50 cm in diameter and 60 cm in height, and the salinity of water is 0.209; distributing a male crab for each net cage, wherein the ratio of female to male of full sib directional mating is 1:1, and the ratio of female to male of half-sib directional mating is 2: 1 or 3: 1; putting the obtained egg-carrying crabs into a 2*667 m<2>*1.5 m outdoor soil pond for overwintering cultivation; in the middle ten-day period of April of next year, pumping out the water in the pond to catch the egg-carrying crabs, putting the egg-carrying crabs into cylindrical net cages weaved by polyethylene soft plastic, wherein the cylindrical net cages are respectively 20 cm in diameter and 50 cm in height and are respectively arranged in net boxes in size of 3*2*1.5 m in other soil ponds respectively, and one egg-carrying crab is arranged in each net box; after the culturing of young crabs is finished, taking out the parents, and performing soil pond cultivation for the juvenile crabs according to the Eriocheir sinensis artificial breeding standard SC/T1099-2007.

The invention relates to a breeding technology for large-size parents of Eriocheir sinensis. The breeding technology comprises the step of selecting the athletic parents of the Eriocheir sinensis, wherein the size of the female crab is above 150g and the size of the male crab is above 200g; the breeding technology is characterized by comprising the following steps: from the last ten-day of October to the first ten-day of November per year, soaking the selected parents of the Eriocheir sinensis into fresh water with tiny running water and sufficient oxygen for 2-3 minutes, and then taking out; after 3-5 minutes, disinfecting by using potassium permanganate; temporarily breeding in an indoor glass reinforced plastic over-wintering pool; starting the over-wintering breeding for the parents of the Eriocheir sinensis in the middle ten days of December; at the beginning of March per year, at a mating moment, controlling the water temperature at 9-12 DEG C, wherein the male and female ratio is (1.5-2):1 and the mating density is 7-13 pieces/m<2>; after oogenesis, continuously aerating and increasing the inflating volume, wherein the breeding temperature for the larva of the Eriocheir sinensis is at 24-25 DEG C and the breeding seawater salinity is 1.8%; and feeding rotifera, fairy shrimp larva and fresh water cladocerans biological baits.

The present invention provides a feed for mitten crab. It is characterized by that said feed contains shell powder, the described shell powder is fresh shell powder made up by using fresh shell. Said feed can effectively promote growth of mitten crab.

The invention discloses a method for breeding Eriocheir sinensis in an indoor water tank. The method comprises the steps: (1) construction of a breeding environment in the indoor test water tank; (2) selection of Eriocheir sinensis; (3) realization of reasonable stocking density; (4) daily management and bait feeding; and (5) management during molting. The method for breeding the adult Eriocheir sinensis in the indoor water tank is developed by combining ecological breeding engineering and aiming at breeding in the indoor water tank according to the characteristics of the breeding biology and the autecology of the Eriocheir sinensis. Proved by the technical practices of the inventor, the adult Eriocheir sinensis breeding in the indoor water tank succeeds, and the method has great significance in the field of breeding and cultivating Eriocheir sinensis.

The present invention provides a formula of an eriocheir sinensis ecology starter feed. The formula is characterized by comprising the following substances, by weight: 22-31% of soybean meal, 24-33% of fish powder, 1-3% of rotifer powder, 1.5-3.5% of cuttlefish powder or a cuttlefish paste, 5-12% of fermentation cottonseed protein, 2.5-7.5% of defatted rapeseed meal, 2-4% of soybean phospholipid powder, 1.5-3% of silkworm pupa powder, 1-1.5% of soybean oil, 1-3% of fish oil, 1.8-2.5% of calcium dihydrogen phosphate, 15-20% of wheat flour, 0.05-0.15% of shelling powder, 0.5-1.5% of a crab trace element additive, 0.1-0.3% of a crab composite vitamin, and 0.1-0.3% of 50% choline chloride. According to the present invention, the rotifer powder prepared from rotifer with characteristics of wide distribution, easy collection and high nutrition is added to the traditional eriocheir sinensis ecology starter feed, such that problems that nutrients of the traditional eriocheir sinensis ecology starter feed are single and nutritional values of the rotifer in the natural environment is not effectively utilized are solved.

The invention discloses a gene engineering preparation and mass spectrometry identification method for eriocheir sinensis crustacean hyperglycemic hormone (Ers-CHH). Energy supplies of various tissues and organs are maintained by CHH through regulating a blood sugar level of a crustacean, and researches on family nerve polypeptide hormones can provide helps for revelation of crustacean metabolism, growth and reproduction regulation mechanisms. In previous studies, an eriocheir sinensis crustacean hyperglycemic hormone gene (Ers-CHH, GenBank registration number: JX485664) is cloned. According to the present invention, a gene engineering method is adopted to successfully obtain recombinant Ers-CHH, wherein a molecular weight is about 9.4 kD, a yield after purification renaturation is 0.4 g/L culture medium, and mass spectrometry identification results verify correctness of the recombinant protein. Due to low CHH content in the crustacean, direct purification enrichment on the CHH is virtually impossible, and a large number of the rare hormone proteins are rapidly obtained by using the gene engineering method in the present invention so as to establish a foundation for subsequent function regulation mechanism research development and application in aquaculture production practice.

A semiautomatic mitten crab packaging machine comprises a mitten crab quick packager for packaging mitten crabs. The mitten crab quick packager comprises a middle cheliped part for being buckled to mitten crab cheliped, a left cheliped part for being bucked to left mitten crab cheliped, a right cheliped part for being buckled to right mitten crab cheliped, a machine frame, a mitten crab feeding mechanism, a mitten crab quick packager feeding mechanism, a mitten crab quick packager closing mechanism and a mitten crab quick packager fixing device, wherein the mitten crab feeding mechanism, the mitten crab quick packager feeding mechanism, the mitten crab quick packager closing mechanism and the mitten crab quick packager fixing device are installed on the machine frame; the mitten crab quick packager fixing device is used for containing the mitten crab quick packager; the mitten crab quick packager feeding mechanism is located above the mitten crab feeding mechanism; the mitten crab quick packager closing mechanism is located in front of the mitten crab feeding mechanism; and the mitten crab quick packager fixing device is located in front of the mitten crab quick packager closing mechanism. The semiautomatic mitten crab packaging machine is high in working efficiency, uniform in quality and safe.

The invention relates to the technical field of molecular biology, in particular to eriocheir sinensis Crustin-1 gene clone and a recombination expression technology thereof. Crustin-1 gene of which the cDNA span is 821bp is cloned from eriocheir sinensis by using an expressed sequence tag (EST) technology and a 3' and 5' end rapid augmentation technology (RACE) and comprises a 315bp open reading frame, polyadenylic acid tailing signals and polyadenylic acid tails, 104 amino acids are coded, the length of a 5' non-coding region is 239bp, the length of a 3' non-coding region is 267bp, and the Crustin-1gene play an important role in the immune defense aspect of the eriocheir sinensis. The invention obtains eriocheir sinensis Crustin-1 protein by using the in-vitro recombination expression technology, the recombination protein has stronger bacteriostatic activity for gram-positive bacteria, the minimal inhibitory concentrations of the recombination protein for micrococcus luteus, bacillus subtilis, micrococcus tetragenus and bacillus thuringiensis are 0.12 mu m, 0.23 mu m, 0.46 mu m and 0.12 mu m respectively, and the recombination protein does not have obvious inhibiting function. The invention can lay the foundation for the disease control of the eriocheir sinensis, the gene assistant breeding and the development of feed additives.

A feed nutritive additive for fattening eriocheir sinensis relates to an aquaculture feed additive. The additive solves the problem that provisioning food cannot meet the needs of eriocheir sinensis parental gonadal development so a feed nutritive additive for fattening is needed to guarantee that the hepatopancreas of the eriocheir sinensis can gain rich nutrition and that the eriocheir sinensis can have good gonadal development as well as have plentiful cream and fat. The technical proposal of the invention which takes phospholipid, fish oil, vegetable oil and livestock animal oil as raw materials is characterized in that the phospholipid, the fish oil, the vegetable oil and the livestock animal oil are mixed well on weight percentage of 2:1:1:1 into the nutritive additive. The invention is used as feed nutritive additive for fattening the eriocheir sinensis.

The invention discloses an Eriocheir sinensis sexual precocity property related SNP marker and its application. The SNP is positioned in a 3'-UTR region of ecdysis inhibition hormone gene MIH(AY310313.1) and is concretely positioned in 3088bp, and base variation information is SNPA3088T T>G. Property-genotype relevance analysis results show that a significant correlation exists between SNP mutation and river crab sexual precocity properties, wherein the sexual precocity property risk of the GG genotype river crab individuals is 2.175 times higher that of TT genotype individuals. The polymorphism SNP marker of the river crab sexual precocity related gene, and can be used for the subsequent and river crab sexual precocity property related genetic and breeding researches.

The invention relates to the field of water purification, in particular to a method for managing pool eutrophication by planting rice. The method for managing pool eutrophication by planting the rice includes the following steps: a pool is aired and cleaned, and pests in the pool are killed; organic fertilizers for fermentation treatment are applied in middle of March; food organisms are started to be cultivated at the beginning of April; the food organisms are placed into the pool; reed rice seedlings are started to be cultivated at the beginning of May; rice seedling transplanting is started to be carried out in the middle of May; urea is applied after rice seedling transplanting is carried out; large-specification juvenile eriocheir sinensis, odontobutis obscura fries and an appropriate number of aristichthysnobilis fishes are input in the middle of June; compound fertilizers are applied at the beginning of July; harvesting is started to be carried out in the middle of November. By means of the method, rice is planted in the pool, the eriocheir sinensis and the odontobutis obscura which are high in economical value are bred in a mixed mode, aquatic products which are high in quality and high in economic value are produced while the water is purified, the price is higher the price of river crabs in the pool, ecological rice is achieved, and the pool cultivation comprehensive benefit is further improved.

The invention discloses a breeding method of three matched breeding systems of Eriocheir sinensis. The breeding method comprises the following steps of: (1) matching an original parent source of the breeding systems; (2) establishing the matched breeding systems: taking weight, rostral teeth and ambulatory leg lengths as breeding targets to establish A, B and C three matched breeding systems; (3)utilizing a group breeding method to carry out parallel breeding on each matched breeding system; (4) estimating breeding effects: breeding the matched breeding systems to the third generation and estimating the breeding effects. By utilizing the breeding method of the matched breeding systems of the Eriocheir sinensis, the breeding systems are constructed and three systems are respectively carried out by the parallel breeding through utilizing the group breeding method to form the breeding systems with different morphological characteristics, different growth speeds, different selection pressures and different hereditary variations; according to the method provided by the invention, the essential problem that the source of parent crab germplasm is bad in the traditional production of river crab larva is solved and conditions for manufacturing breeding strains or improved varieties which are polymerized by excellent germplasm are created.

The invention discloses an Eriocheir sinensis EsSXL gene, an amplification primer group thereof and an amplification method. A full-length cDNA (complementary Deoxyribose Nucleic Acid) sequence of the EsSXL gene is cloned from an Eriocheir sinensis testis tissue by using the primer group provided by the invention, and the EsSXL gene comprises two variable spliceosomes EsSXL1 and EsSXL2, and lays an important foundation for the research of the important function of the function of the Eriocheir sinensis EsSXL gene in a sex differentiation process of the Eriocheir sinensis EsSXL gene.

A winter temporary culture method of commercial eriocheir sinensis includes the steps of 1, performing strengthened fattening to crabs in temporary culture; 2, selecting a pond for temporary culture;3, regulating water quality of the pond for temporary culture; 4, selecting the crabs in temporary culture; 5, transporting the crab in temporary culture; 6, setting net cages; 7, putting in commercial crabs; 8, performing daily management; 9, fishing and selling. The winter temporary culture method of commercial eriocheir sinensis solves the problems such as difficulty in selling and low price due to the fact that existing commercial eriocheir sinensis is on the market concentrately, and low amount of storage of the commercial crabs and high death rate caused by the conventional winter temporary culture method, and the like.

The invention relates to an eriocheir sinensis blood DNA extraction method for Southern hybridization analysis, which is characterized by comprising the following steps of: sucking ACD anticoagulant by using a syringe, inserting the syringe into a soft membrane of a walking leg base joint of eriocheir sinensis to suck blood, and fully mixing the ACD anticoagulant and the blood uniformly to obtain mixed solution; centrifuging the mixed solution, removing supernate and leaving blood cell sediment; suspending the blood cell sediment into lysing solution to obtain mixed solution; adding Tris saturated phenol into the mixed solution, shaking the solution uniformly and centrifuging the solution; adding chloroform into the supernate, shaking the mixture uniformly and then centrifuging the mixture, and taking the supernate; adding ammonium acetate and anhydrous ethanol into the supernate, shaking the mixture uniformly and then centrifuging the mixture, and collecting DNA; and washing the DNA by using 70 percent ethanol, and airing and dissolving the DNA. The DNA extracted by the method has high concentration, and can meet a Southern hybridization method.

The invention discloses a microbial feed additive capable of improving the meat quality of eriocheir sinensis and a preparation method thereof. The microbial feed additive comprises bacillus subtilis (Bacillus subtilis), bacillus licheniformis (Bacillus licheniformis) and bacillus cereus (Bacillus cereus). Beneficial microorganisms are utilized for degrading protein and fat an helping digestion, the conversion rate of a feed is improved, growth is promoted, the content of crude protein and crude fat of the eriocheir sinensis can be significantly improved and the content of total amino acids, essential amino acid and unsaturated fatty acid of the whole eriocheir sinensis is significantly improved.

The invention discloses an SNP mark related to the weight of eriocheir sinensis and application of the SNP mark. The SNP mark related to the weight of the eriocheir sinensis is located on the5'- side wing area of an MIH gene, the specific position is the 372bp part of the MIH gene, and nucleotide variation information is SNP g.372T>C. Obvious relevance exists between the SNP position point and the weight trait of the eriocheir sinensis species, and the average weight of the CC gene type individual is obviously higher than the average weight of the TT gene type individual. SNP g.372T>C genetic typing is carried out on a river crab breeding colony and morphology characteristic analysis is combined so that the individual with the gene type of CC can be preferably selected as a breeding parent, and the research has the significant guiding meaning on breeding of a novel large-specification river crab variety.

The invention relates to an Eriocheir sinensis anti-lipopolysaccharide factor gene cloning and in vitro recombinant expression technology of molecular biology, and the Eriocheir sinensis anti-lipopolysaccharide factor gene has the base sequence in a sequence table of SEQ ID No: 1. The Eriocheir sinensis anti-lipopolysaccharide factor is studied by utilizing a constructed Eriocheir sinensis cDNA library and adopting the RACE technology and the in vitro recombinant expression technology, the cDNA sequence of the anti-lipopolysaccharide factor is cloned from the Eriocheir sinensis for the first time, the gene has the sequence which is indicated in the sequence table, and the prokaryotic recombinant technology is utilized for expressing a recombinant protein with stronger bactericidal activity.

The invention relates to the fields of molecular biology and biological technology, and discloses Eriocheir sinensis GnRH (gonadotropin-releasing hormone) analogues with respective amino acid sequence of QSYHFSHGWKP and QAYHFSHGWKP . Compared the the amino acid sequences of the two subtypes of Eriocheir sinensis GnRH analogues with those of the GnRH of other species, the results show that the former and the latter have similar amino acid conservative sites; the the sequence structures of the Eriocheir sinensis GnRH analogues are similar to that of invertebrate GnRH; and the Eriocheir sinensis GnRH analogues have similar functions as vertebrate GnRH, can stimulate breakdown of oocyte germinal vesicle and have distinct stimulative effect on crab oocyte maturation and important application value for artificial ripening of the Eriocheir sinensis.

The invention relates to the technical field of breeding, in particular to an eriocheir sinensis white spot syndrome virus specificity PCR detection kit and method. The eriocheir sinensis white spot syndrome virus specificity PCR detection kit comprises 1.0 mL of a 2*reaction mixture buffer solution, preferably-designed upstream or downstream primers, Taq DNA polymerase, positive control fluid, negative control fluid and ddH2O. The novel and rapid PCR detection kit and method overcome the defects of an existing eriocheir sinensis white spot syndrome virus detection method. The eriocheir sinensis white spot syndrome virus specificity PCR detection kit and method are practicable for clinic diagnosis on the eriocheir sinensis white spot syndrome virus, have the advantages of being rapid, accurate and special, meet the requirement of clinic diagnosis, and provide convenience for eriocheir sinensis white spot syndrome virus detection.

The invention discloses an SNP locus significantly relevant to the precocious puberty trait of eriocheir sinensis and the application. The SNP locus is positioned in a Vg gene 5'-flanking region-437bpposition of the eriocheir sinensis, and nucleotide variation information is SNP g.-437T) G. PCR primer used for detecting the SNP locus is SEQ ID NO.1 and SEQ ID NO.2, and restriction enzyme is MboI.The SNP locus has significant correlation with precocious puberty of the eriocheir sinensis, and the risk of precocious puberty trait of GG gene eriocheir sinensis individual is obviously smaller than that of precocious puberty trait of TT gene individual. The SNP locus and the PCR primer used for genetic typing are applied to the predication of the risk of precocious puberty of the eriocheir sinensis and/or the variety breeding of the eriocheir sinensis.