43 results about "Eriocheir" patented technology

The invention relates to the technical field of RNA interference, and particularly discloses an RNA interference fragment of a crustacean hyperglycemia hormone gene and application of the RNA interference fragment. The RNA interference fragment provided by the invention is injected into a third step foot of a river crab in an injection manner and is used for interfering the mRNA expression quantity of a hyperglycemia hormone gene CHH of a crustacean of the river crab. The fighting behavior of the eriocheir sinensis is effectively controlled, the yield in large-scale breeding of the eriocheir sinensis is improved, and economic losses caused by incomplete crabs due to fighting of the eriocheir sinensis are reduced.

The invention discloses a feed additive for promoting muscle and gonad development of eriocheir sinensis and application of the feed additive, and relates to the technical field of aquatic feed. The feed additive comprises cholesterol and omega-3 high unsaturated fatty acid. Each kilogram of the feed comprises the following feed additives by weight: 0.4 to 4.5 g of cholesterol and 3.5 to 4.5 g of omega-3 high unsaturated fatty acid. According to the feed additive, 4.022 g / kg omega-3 high unsaturated fatty acid (omega-3 HUFA) is supplemented into feed containing 4.114 g / kg cholesterol, so that the weight gain rate, the gonad index and the meat percentage of female crabs in the fattening period can be remarkably increased; the protein nutrition deposition capability of gonads and muscles is obviously improved; when the feed added with the feed additive is used in the fattening period of the eriocheir sinensis, the feed additive has importance on nutrient deposition and quality improvement of female crabs; the production cost of farmers can be reduced, the product value is improved, and technical support is provided for healthy development of the hairy crab industry.

The application of the primer for detecting the anti-disease gene of the Eriocheir sinensis in identifying the disease resistance of the parent of the Eriocheir sinensis comprises the following steps: (1) respectively extracting RNA (Ribonucleic Acid) of the Eriocheir sinensis, and carrying out reverse transcription to obtain cDNA (Complementary Deoxyribonucleic Acid) of the Eriocheir sinensis; (2) designing a primer combination according to the sequence of the lysozyme gene of the eriocheir sinensis and the sequence of the C-type lectin 4 gene, performing PCR amplification on the cDNA sample of the eriocheir sinensis by utilizing the primer combination to obtain a target fragment, and detecting and confirming that the target fragment is amplified by utilizing agarose gel electrophoresis; (3) obtaining a cDNA sequence by a first-generation sequencing means, carrying out sequence comparison, identifying SNP sites in gene coding regions of the lysozyme gene and the C-type lectin 4 gene, and analyzing the two genes and a combination thereof; and comparing the differences of the disease resistance of the genotypes, and screening to obtain the genotype with the strongest disease resistance and the combined marker primer thereof.

The invention discloses a method for distinguishing good crabs according to shell color changes, which enables the shell color of the good crabs to rapidly become light through a method of changing a photoperiod so as to achieve the purpose of distinguishing the good crabs. Comprising the following five operation steps: screening eriocheir sinensis and adjusting shell color; carrying out an illumination experiment; analyzing the shell color, testing aggressiveness, carrying out a fighting experiment, and distinguishing good crabs; and drawing a conclusion. According to the method, the gene polymorphism between the good crabs and the non-good crabs is further distinguished and analyzed through a scientific method, an important group material is provided for molecular breeding of the eriocheir sinensis, and a new eriocheir sinensis variety (the non-good crabs) can be finally cultured and used as a main culture object. Meanwhile, according to the characteristics of the good crabs and the non-good crabs, different breeding environments are selected for separate breeding. The non-good crab groups are subjected to high-density culture in the pond, so that the yield is increased; low-density cage culture or single culture is carried out on the good-fighting crab group, so that the individual quality is further improved, and the unit price is increased.

The application discloses bacillus subtilis G190 and application thereof in Eriocheir sinensis breeding. After soybean meal is fermented by the bacillus subtilis G190, aquatic product feed is prepared, and the Eriocheir sinensis is bred by using the feed, so that the survival rate and flavor substances of the Eriocheir sinensis can be remarkably improved. The effective implementation of the research can provide scientific theoretical basis for the effect, regulation mechanism and popularization and application of the probiotic bacillus feed, and effectively serve further development of sustainable ecological breeding technology of the Eriocheir sinensis.

The invention belongs to the technical field of biological detection, and particularly relates to an Eriocheir sinensis white spot virus QPCR kit and a use method and application thereof.The Eriocheir sinensis white spot virus QPCR kit comprises a primer pair used for detecting the Eriocheir sinensis white spot virus, and the primer pair comprises an upstream primer WSSV-F shown as SEQ ID NO: 1 and a downstream primer WSSV-R shown as SEQ ID NO: 2. The detection method constructed according to the primer pair has the advantages of simplicity and convenience in operation, strong specificity, good repeatability and the like, the result can be judged according to an amplification curve immediately after the reaction is finished, the simultaneous detection and identification of the Eriocheir sinensis white spot virus are realized, and the method not only can save the time cost, but also can reduce pollution, and has extremely high application value.

The present invention discloses the application of a diplotype of a weight-associated SNP site in breeding of Chinese mitten crab (Eriocheir sinensis), belonging to the technical field of aquatic breeding. The diplotype is randomly formed by haplotype I, haplotype II, and haplotype III, wherein the bases at exons 442 and 793 of the bsk gene of haplotype I are C and G, respectively; the bases at exons 442 and 793 of the bsk gene of haplotype II are C and C, respectively; the bases at exons 442 and 793 of the bsk gene of haplotype III are A and C, respectively; the homozygous diplotype V randomly formed by haplotype III has significant advantages over other diplotypes in terms of average body weight and bsk gene expression; the homozygous diplotype V can be used for molecular-assisted breeding of new fast-growing strains of Chinese mitten crab (Eriocheir sinensis), accelerating the breeding process of fast-growing and improved varieties of Chinese mitten crab (Eriocheir sinensis).

The invention discloses bacillus subtilis G190 and application of the bacillus subtilis G190 in culture of eriocheir sinensis. The bacillus subtilis is used for fermenting soybean meal to prepare an aquatic product feed, the feed is used for breeding the eriocheir sinensis, and the survival rate and flavor substances of the eriocheir sinensis can be remarkably increased. The effective implementation of the research can provide a scientific theoretical basis for the action effect, regulation and control mechanism, popularization and application of the probiotic bacillus feed, and effectively serves the further development of the sustainable ecological breeding technology of the eriocheir sinensis.

The invention discloses a method for culturing Eriocheir sinensis with oyster shells, which comprises the following steps: S1, pretreating a crab pond, and laying a microporous oxygenation pipeline; s2, collecting the waste oyster shells, and performing cleaning, disinfection and drying treatment; s3, the oyster shells treated in the step S2 are stacked in a long ridge shape in the crab pond in the direction parallel to the micropore oxygenation pipeline, and the whole crab pond is disinfected; s4, waterweed is planted in the oyster shell ridge direction in the middle ten days of January; s5, crab species are stocked in early February, so that the environment richness of the crab pond is improved, self-killing of the river crabs is reduced, the survival rate is increased, and the adult crab breeding technology is more environmentally friendly and efficient.

The invention relates to the technical field of aquaculture, in particular to a method for improving the growth performance and nutritional quality of female eriocheir sinensis. The method comprises the following steps: pretreating phaeodactylum tricornutum to obtain pretreated phaeodactylum tricornutum; in the feeding process of female eriocheir sinensis, basic feed is fed into a feeding water body, and the pretreated phaeodactylum tricornutum is additionally fed; wherein the feeding amount of the pretreated phaeodactylum tricornutum is dynamically adjusted according to the change of the feeding water temperature. By adding the phaeodactylum tricornutum, the food source of the eriocheir sinensis is directly changed, and the final weight, gonad index, meat percentage, total edible rate and fatness of female crabs are remarkably improved; protein, amino acid and vitamins which are rich in phaeodactylum tricornutum can directly optimize the growth and quality of the eriocheir sinensis, and especially under the condition of high-density culture, the quality of the eriocheir sinensis is correspondingly improved while the growth performance of the eriocheir sinensis can be remarkably improved.

The invention relates to the technical field of eriocheir sinensis breeding, in particular to application of a new wheat-eriocheir sinensis ecological planting and breeding mode in improving the quality of adult crabs, which comprises the following steps: planting wheat in a field, digging ditches around to protect eriocheir sinensis, and performing field operation in the growth period of wheat; after wheat is harvested, straw is removed, then land is ploughed, and water is injected for grass planting; after 15 days, Chinese mitten crabs are bred in the wheat field; through a new wheat-eriocheir sinensis ecological planting and breeding mode, the maturity and quality of adult eriocheir sinensis are improved.

The invention relates to the technical field of breeding of adult eriocheir sinensis, in particular to application of a new wheat-crab ecological planting and breeding mode to improvement of microbial communities in an adult crab breeding environment, which comprises the following steps: planting wheat in a field, digging ditches around to protect eriocheir sinensis, and performing field operation in the growth period of wheat; after wheat is harvested, straw is removed, then land is ploughed, and water is injected for grass planting; after 15 days, Chinese mitten crabs are bred in the wheat stubble field; through the new wheat-crab ecological planting and breeding mode, the microorganism composition of the culture environment of the eriocheir sinensis is improved, the change rule of microorganisms of water quality, water body and bottom mud under the new wheat-crab ecological planting and breeding mode is mastered, and the culture mode of adult crabs is enriched.

The invention relates to a male and female specific DNA (deoxyribonucleic acid) sequence for identifying genetic sex of eriocheir sinensis and application of the male and female specific DNA sequence. The nucleotide sequence of the male and female specific DNA sequence is shown as SEQ ID NO: 1; the sex identification method is realized by identifying the male and female specific DNA sequence, and comprises the following steps: extracting genome DNA of the eriocheir sinensis; the method comprises the following steps: carrying out PCR (Polymerase Chain Reaction) amplification by taking genome DNA as a template, wherein primer sequences for PCR amplification are shown as SEQ ID NO: 2 and SEQ ID NO: 3; agarose electrophoresis detection is carried out on amplification products, the products with male and female specific DNA sequence amplification bands are female eriocheir sinensis, and otherwise, the products are male eriocheir sinensis. The primer pair developed and designed based on the male and female specific DNA sequence is proved to have high accuracy and reliability in early sex identification of the eriocheir sinensis, and important technical support is provided for sex control breeding, unisexual breeding and genetic improvement of the eriocheir sinensis.

The invention provides a primer probe group, a kit and a detection method for detecting microsporidia of eriocheir sinensis, and belongs to the technical field of pathogen detection. The invention provides a primer probe group for detecting microsporidia of eriocheir sinensis. The primer probe group comprises a primer pair and a TaqMan probe, the nucleotide sequences of the primer pair and the TaqMan probe are as shown in SEQ ID NO: 1 to SEQ ID NO: 3. In the invention, the primer probe group can specifically detect the eriocheir sinensis microsporidia, and does not have cross reaction with aeromonas veronii, vibrio parahaemolyticus, eriocheir sinensis spiroplasma, white spot syndrome virus and siniperca chuatsi iridovirus. The lowest detection limit of the primer probe group is 4.6 * 10 < 1 > copies / mu L, the sensitivity is improved by 1000 times compared with that of common PCR, and great detection advantages are shown.

The invention provides paste slurry type pellet feed for eriocheir sinensis and a production method and device of the paste slurry type pellet feed. According to the invention, compounded cuttlefish paste, shrimp paste and enzymolysis fish paste form external spraying paste, base material particles are put into a rotating cylinder to form dynamic rolling, and the external spraying paste is sprayed to the surfaces of the rolling base material particles through a nozzle of a spraying pipe, so that the external spraying paste uniformly coats the base material particles and is prevented from caking and falling off; finally, collaborative optimization of food calling enhancement and structural stability is achieved, and the technical defects that traditional pellet feed is insufficient in food calling, poor in water resistance and low in production efficiency are systematically overcome, so that the pellet feed has the chilled fish-grade food calling performance and the stability of commercial feed; an industrial feed solution with efficient ingestion promotion and lasting water resistance is provided for sustainable development of large-scale culture of the eriocheir sinensis.

The present application relates to the technical field of RNA interference, and particularly discloses a RNA interference fragment of a crustacean hyperglycemic hormone gene and application thereof. The RNA interference fragment is injected into the third step of a river crab in a manner of injection, and is used for interfering with mRNA expression of a crustacean hyperglycemic hormone gene CHH of the river crab. The fighting behavior of Eriocheir sinensis is effectively controlled, which is beneficial to the yield improvement of large-scale breeding of Eriocheir sinensis and reduction of economic losses caused by the incomplete Eriocheir sinensis due to fighting.

The utility model discloses a sealed eriocheir sinensis tissue sample collection box, apply in sample collection technical field, including box, the rear side of box is connected with the apron through the axle pin swing, the inner chamber fixed mounting of box has the deposit storehouse, the both sides of box front side all are connected with the clamping component through the axle pin swing, the clamping component includes the clamping plate, the rear side of clamping plate is fixed with apron mounting, the both sides of box and apron front side and rear side all are clamped with supplementary sealing component. When needing to increase the sealing property of the collection box sealed through the clamping component, the user unfolds the supplementary sealing component at this moment, the positioning shell in supplementary sealing component is contacted with the box, and is fixed after through bolt and box, then clamps the positioning plate and apron, fixes supplementary sealing component between box and apron through the resilience of limiting spring, to increase the sealing property of the collection box sealed through the clamping component.

The invention relates to the technical field of aquaculture, in particular to a breeding method for improving the disease resistance of eriocheir sinensis larvae. The method comprises the following steps: soaking and stimulating egg-carrying crabs of the Eriocheir sinensis twice by using seawater containing inactivated Meischwa yeast with the concentration of 1 * 10 < 5 > CFU / mL, and hatching the stimulated egg-carrying crabs to generate daphnia larvae, namely the disease-resistant Eriocheir sinensis larvae. By adopting the breeding method provided by the invention to breed the egg-carrying crabs, the development of Eriocheir sinensis embryos can be promoted, and the resistance of zoea larvae to infection of Meischwa's yeast is improved.

The invention discloses a method for tracing the origin of eriocheir sinensis based on flavor characteristics, which comprises the following steps of: firstly, extracting target organ tissues of an eriocheir sinensis sample; then, volatile organic compounds in target organ tissues are detected through headspace gas chromatography-ion migration spectrometry, and obtained volatile substance spectral data are subjected to data preprocessing and feature extraction; and finally, inputting the component characteristic matrix into a component analysis model for identification, and inputting an obtained target characteristic vector set into an SVM model for identification to obtain the production place of the eriocheir sinensis sample. According to the method, the volatile organic compound characteristics obtained through gas chromatographic analysis are combined with the statistical model and the learning model, so that the production place of the eriocheir sinensis is traced, and the accuracy and reliability of the production place traceability are improved.

The invention discloses application of a diplotype of a body weight associated SNP (Single Nucleotide Polymorphism) site of eriocheir sinensis in breeding, and belongs to the technical field of aquatic breeding. The haplotype is randomly formed by a haplotype I, a haplotype II and a haplotype III, basic groups at the exons 442 and 793 of the bsk gene of the haplotype I are C and G respectively, basic groups at the exons 442 and 793 of the bsk gene of the haplotype II are C and C respectively, and basic groups at the exons 442 and 793 of the bsk gene of the haplotype III are A, C and G respectively. Compared with other diplotypes, the homozygous double V type randomly formed by the haplotype III has remarkable advantages in average weight and bsk gene expression, and can be used for molecular assisted breeding of a fast-growing new strain of the eriocheir sinensis, so that the breeding process of fast-growing improved varieties of the eriocheir sinensis is accelerated.

The invention discloses an antibacterial peptide Hyastatin gene of Eriocheir sinensis as well as a recombinant mature peptide and application of the antibacterial peptide Hyastatin gene. A unigene sequence obtained by transcriptome sequencing is utilized, a 5'and 3 'terminal rapid amplification technology is utilized to amplify in an Eriocheir sinensis blood lymphocyte cDNA library to obtain an antibacterial peptide Hyststin gene full-length sequence, and the gene plays an important role in the innate immune defense aspect of Eriocheir sinensis. According to the present invention, the Eriocheir sinensis antibacterial peptide Hyastatin recombinant mature peptide is obtained by using the in vitro recombinant expression technology, the antibacterial peptide Hyastatin recombinant mature peptide has significant inhibition effect on the common aquaculture pathogenic bacteria aeromonas hydrophila, and the foundation can be laid for Eriocheir sinensis disease control, medical product development and feed additive application.

The invention discloses a construction method and application of an Eriocheir sinensis gut-brain axis research model, according to the method, metronidazole, neomycin and vancomycin are added to commercial feed according to the mass ratio of 10: 10: 5 g / kg to prepare compound antibiotic feed, and the Eriocheir sinensis is continuously fed for 96 h. According to the method, a standardized research model capable of synchronously and controllably presenting the causal relation of'intestinal flora disturbance-nervous system response 'is constructed in crustacean for the first time. The model is short in construction period, high in stability and high in repeatability, and a key research tool is provided for deeply analyzing the enteric-cerebral axis interaction mechanism of the eriocheir sinensis and even crustacean, screening neural active substances and evaluating neurotoxicity of environmental stress.

The present application belongs to the technical field of biological information analysis, and particularly relates to a microsatellite marker of a temperature regulation related functional gene of Scylla paramamosain, a detection method, a kit, an analysis method, a screening method and a breeding method. Through transcriptome data analysis, the present application screens a temperature adaptability related functional gene, develops a microsatellite marker of the temperature regulation related functional gene of Scylla paramamosain, and provides polymorphic primers, establishes a technical system and a detection method for development of the microsatellite marker of the functional gene of Scylla paramamosain. The present application not only lays a foundation for Scylla paramamosain genetic diversity research, genetic map construction and evolution analysis, but also provides valuable and reliable molecular markers for breeding of fine varieties of Scylla paramamosain.

The application discloses a siRNA targeting for Eriocheir sinensis RDH11 gene and application thereof, and belongs to the technical field of molecular biology of aquaculture. The siRNA is siRDH11 targeting for the Eriocheir sinensis RDH11 gene, and a sequence is shown as SEQ ID NO: 1 or 2; the siRNA is used for preparing a drug for inhibiting fighting behavior of the Eriocheir sinensis, and the siRNA is used as an active ingredient; in particular, the drug is injected into the Eriocheir sinensis in an injection mode, and the expression amount of the RDH11 gene in a brain ganglion or a thoracic ganglion of the Eriocheir sinensis is inhibited or reduced through the siRNA. The siRNA targeting for the Eriocheir sinensis RDH11 gene is developed to realize molecular precise regulation of fighting behavior of the Eriocheir sinensis, and the problem that the aquaculture yield is reduced due to the fighting behavior of the Eriocheir sinensis in the aquaculture process is solved, and the application has important industrial value.

The invention discloses a feed for improving the quality of eriocheir sinensis and a preparation method of the feed, and belongs to the technical field of crab feeds. The feed comprises the following raw materials in parts by weight: 30-40 parts of fish meal, 5-15 parts of chicken meal, 8-12 parts of soybean meal, 4-6 parts of peanut meal, 2-5 parts of egg white powder, 1-5 parts of pig protein powder, 1-5 parts of sesame powder, 15-20 parts of starch, 5-8 parts of soybean oil, 0.5-1 part of 50% choline chloride, 0.5-1.5 parts of monocalcium phosphate, 0.5-1 part of vitamin mineral premix and 1-3 parts of zeolite powder. The crab feed obtained by the invention can obviously improve the quality of the eriocheir sinensis.

The invention relates to an Eriocheir sinensis origin identification method based on an electronic tongue. The method comprises the following steps: acquiring a preset number of Eriocheir sinensis from at least two preset origin; obtaining a crab meat sample based on a preset gram weight, and preparing a sample solution corresponding to each producing area; obtaining analysis solutions based on the preset capacity, and detecting the analysis solutions one by one by using the electronic tongue to obtain taste feature values of the analysis solutions corresponding to the preset taste features; based on the taste feature values corresponding to the analysis solutions, SPSS is used for discriminant analysis, and discriminant functions corresponding to the producing areas are constructed respectively; and identifying the eriocheir sinensis of an unknown producing area in combination with the discrimination function. The method has the characteristics of convenience, objectiveness and rapidness.

The invention belongs to the technical field of biological detection, and particularly relates to an absolute fluorescent quantitative PCR (Polymerase Chain Reaction) detection kit for detecting fasciculago fasciculatus. The invention provides a primer pair for detecting a parasite, namely fasciculago fasciculatus parasitized in Eriocheir sinensis, which is characterized in that an upstream primer and a downstream primer with the amplified fragment size of 257bp are synthesized according to a target gene sequence of the fasciculago fasciculatus. The invention also provides a kit containing the primer pair and used for detecting the fasciculatorula fasciculatorula. The kit provided by the invention can quickly diagnose the content of parasitic fasciculago fasciculatus in the Eriocheir sinensis body through the specific primer of fasciculago fasciculatus in the Eriocheir sinensis body and SYBR Green fluorescent quantitative PCR detection, so that the sensitivity and the specificity of the detection of fasciculago fasciculatus disease of the Eriocheir sinensis are enhanced to a great extent, and missing detection and false detection are avoided.

The invention discloses an Eriocheir sinensis ELOVL7 gene and application thereof, the nucleotide sequence of the Eriocheir sinensis ELOVL7 gene is as shown in SEQ ID NO: 1, and the amino acid sequence of protein encoded by the Eriocheir sinensis ELOVL7 gene is as shown in SEQ ID NO: 2. The Eriocheir sinensis ELOVL7 gene sequence is successfully obtained through a PCR technology for the first time, the function of the ELOVL7 gene is verified through an in-vitro experiment, it is proved that the gene serves as an elongase gene to play a role in the Eriocheir sinensis fatty acid synthesis process, the function of promoting LC-PUFA synthesis is achieved, a foundation is laid for studying the LC-PUFA anabolism mechanism of the Eriocheir sinensis, and the Eriocheir sinensis ELOVL7 gene has a good application prospect. And a reference is provided for the functional research of other crustacean ELOVL7.

The utility model is suitable for the technical field of Eriocheir sinensis culture, and provides a Eriocheir sinensis storage and culture pool, which comprises: a storage and culture pool body for culturing Eriocheir sinensis, wherein the storage and culture pool body comprises a plurality of culture pools, and the plurality of culture pools are used for placing and culturing Eriocheir sinensis; a mounting frame fixedly mounted on the storage and culture pool body; a feeding box assembled on the mounting frame, wherein the feeding box is used for placing food for feeding Eriocheir sinensis; and a feeding mechanism assembled on the feeding box, wherein the feeding mechanism is used for feeding the food placed in the feeding box into the storage and culture pool body. The Eriocheir sinensis storage and culture pool can culture Eriocheir sinensis, and can automatically feed the cultured Eriocheir sinensis, thereby reducing the labor burden of the staff and improving the culture efficiency.

The invention discloses a construction method and application of an Eriocheir sinensis intestinal flora deletion model, the method comprises the following steps: feeding Eriocheir sinensis with a feed mixed with mixed antibiotics for 96 hours to obtain the Eriocheir sinensis intestinal flora deletion model; the mixed antibiotic is prepared from metronidazole, neomycin and vancomycin. A plate culture method verifies that the optimized core antibiotic concentration group can enable the clearance rate of intestinal content florae to reach 95% or above and the clearance rate of fecal florae to reach 90% or above. According to the method, the intestinal flora deletion model of the eriocheir sinensis is constructed for the first time, the method has the advantages of being high in removal efficiency, good in repeatability, small in stress and the like, the flora is restorable after drug administration is stopped, and a standardized experimental tool is provided for studying the intestinal-brain axis mechanism, physiological metabolism and healthy breeding of crustacean.