274 results about "TaqMan" patented technology

The invention belongs to the technical field of aquatic pathogen diagnosis, and particularly relates to a Taqman multiple fluorescent quantitative PCR (Polymerase Chain Reaction) detection system and kit for common vibrio pathogens in aquaculture as well as application and a detection method. The system comprises primers and probes for detecting four common vibrio pathogens in aquaculture, the 5'end of each probe is modified with different fluorophores, the 3 'end of each probe is modified with corresponding quenching groups, and the common vibrio pathogens comprise vibrio anguillarum Va, vibrio alginolyticus Val, vibrio harveyi Vh and vibrio scophthalmi Vsc.

The invention discloses a sweet potato arbuscular disease phytoplasma TaqMan fluorescent quantitative detection primer, a kit and a detection method, the primer and a probe group are specially used for specific detection of sweet potato arbuscular disease phytoplasma; the invention also designs a fluorescence quantification (QPCR) kit based on the primer probe group. The detection primer group is as follows: a forward primer F as shown in SEQ ID No: 1, a reverse primer R as shown in SEQ ID No: 2, and a probe primer Probe as shown in SEQ ID No: 3; the sweet potato arbuscular disease phytoplasma can be specifically amplified within 1 hour by extracting the DNA of the sweet potato plant infected with the phytoplasma and carrying out QPCR, and the detection sensitivity is 10 copies / uL. According to the TaqMan QPCR detection technology established for the sweet potato arbuscular disease phytoplasma, the problems that an existing sweet potato arbuscular disease phytoplasma detection method is time-consuming and labor-consuming, poor in specificity and sensitivity, easy to cause pollution and the like are solved, and a technical basis is provided for early diagnosis of the sweet potato arbuscular disease phytoplasma.

The embodiment of the invention discloses a probe primer combination for identifying TaqMan real-time fluorescent quantitative PCR of ampullaria gigas, a kit and application. Comprising a probe, an upstream primer and a downstream primer, the nucleotide sequence of the probe is 5 '-FAM-CCTTCTCTATTACTACTATTA-MGB-3', the nucleotide sequence of the upstream primer is 5 '-CTTTCCGCGTCTAATAACATG-3', and the nucleotide sequence of the downstream primer is 5 '-CATCAGTTCCAGCACCACTC-3'. The TaqMan probe real-time fluorescent PCR probe and primer combination established by the invention has excellent specificity and sensitivity in the aspect of identifying the species of the ampullaria gigas, the detection accuracy is greatly improved, the detection time is shortened, the effects of stability, reliability, short period and low cost are achieved, and the primer combination has good application prospects in rapid and accurate identification of the ampullaria gigas. The method is of great significance in promoting related prevention and research work.

The invention designs a carbapenem drug-resistant gene nucleic acid fluorescent PCR method detection kit and application thereof, the kit uses Taqman fluorescent probe multiple amplification technology to detect carbapenem drug-resistant genes, the kit comprises a nucleic acid reaction liquid Mix1 and a nucleic acid reaction liquid Mix2, the nucleic acid reaction liquid Mix1 is used for detecting drug-resistant genes IMP, NDM and OXA48, the nucleic acid reaction liquid Mix2 is used for detecting drug-resistant genes IMP, NDM and OXA48, and the nucleic acid reaction liquid Mix2 is used for detecting drug-resistant genes IMP, NDM and OXA48. The nucleic acid reaction liquid Mix2 is used for detecting drug resistance genes VIM, KPC and OXA23. The method is high in detection specificity, and the detection result is visual and easy to interpret. Clinical doctors are assisted to judge respiratory tract pathogen infection more comprehensively and more accurately. The kit covers six carbapenem drug resistance genes, is more comprehensive and accurate in detection, and is high in specificity and sensitivity. Meanwhile, multiple sample types can be detected, a sputum sample, an excrement sample and a rectum swab sample are detected through paramagnetic particle extraction, and pure bacterial colonies are directly detected without extraction.

The invention discloses a nucleic acid combination product for detecting various respiratory pathogens, a kit containing the nucleic acid combination product and a method. The nucleic acid combination product comprises a primer pair and a probe, wherein the nucleotide sequences of the primer pair and the probe are respectively shown as SEQ ID NO: 1-21. According to the nucleic acid combination product, on the technical basis of Taqman fluorescent quantification, seven targets can be detected by one tube, so that the time and energy of operators are greatly shortened, and the nucleic acid combination product is convenient and rapid, is not easy to make mistakes, and is simpler and easier to understand in result interpretation.

The invention relates to a multiplex PCR (Polymerase Chain Reaction) primer probe combination, a kit and a detection method for human red blood cell RHCE genetic typing detection, and belongs to the field of biomedicine clinical molecular detection. The invention provides a multiplex PCR (Polymerase Chain Reaction) primer probe combination for human red blood cell RHCE genetic typing detection, and further provides a kit containing the multiplex PCR primer probe combination. The method has the technical advantages that the RHCE gene variation site specific primer and probe are designed through an improved ARMS technology and a homologous sequence specific base method, so that higher specificity and accuracy are realized; only four-hole reaction is needed, detection of RHC, RHc, RHE, RHe, CW- / CW +, CX- / CX +, V-VS- / V + VS + and V + / V-can be completed at a time, and higher flux and efficiency are achieved; according to the method, multiple fluorescent PCR is combined with a Taqman probe technology, qualitative typing detection is carried out on the human red blood cell RHCE gene, and compared with a traditional method, higher sensitivity, higher experiment speed, higher accuracy and a wider application range are shown.

The invention discloses a TaqMan real-time fluorescent quantitative RT-PCR (Reverse Transcription-Polymerase Chain Reaction) detection fluorescent probe and kit for corn necrosis stripe virus and application, and relates to the technical field of virus detection. The nucleotide sequence of the fluorescent probe provided by the invention is 5 '-FAM-CGCTGGTACATAGGATGGCAAC-TAMRA-3', and the nucleotide sequence of the fluorescent probe provided by the invention is shown in the description. The nucleotide sequence of the primer is as follows: an upstream primer: 5 '-CCTGCTCCTCTGCTATTCG-3'; a downstream primer: 5 '- The downstream primer is 5 '-CAGACTTCCCTTGGCAGTGT-3', and the downstream primer is 5 '- The kit at least comprises the fluorescent probe and the primer. The TaqMan real-time fluorescent quantitative RT-PCR detection method established by the invention is simple and convenient to operate, strong in specificity, high in sensitivity and good in repeatability, is not only suitable for qualitative identification, but also can realize accurate quantitative analysis; the sensitivity is at least 100 times higher than that of a common RT-PCR detection method. The problem that in the prior art, a TaqMan real-time fluorescent quantitative RT-PCR detection method for detecting the corn necrotic stripe virus is lacked can be solved.

The invention belongs to the technical field of biomedicine, and relates to a kit for rapidly detecting hiPSC residues in hiPSC derived cells and application of the kit. The kit comprises six components, namely an NANOG gene primer probe composition, a TaqMan Fast qPCR (Polymerase Chain Reaction) premixed solution, a standard quality particle, a positive reference substance, a sensitivity reference substance and a sample dilution buffer solution, according to the kit, the NANOG gene sequence serves as a template, the designed primer pair and probe have high specificity, absolute quantitative detection can be conducted on hiPSC residues in an intermediate product, a semi-finished product and a finished product (obtained through differentiation of an endoderm, a mesoderm and an ectoderm) of a human induced pluripotent stem cell derived cell preparation, and compared with other detection modes such as a culture method, the detection method has the advantages that the detection efficiency is high, and the detection cost is low. False positive results caused by a detection method are avoided, and the method has the detection characteristics of simplicity and rapidness in operation, high specificity and high sensitivity.

The invention discloses a quadruple Taqman qPCR reagent for detecting food-borne pathogenic bacteria in aquatic products, the food-borne pathogenic bacteria are vibrio parahaemolyticus, vibrio vulnificus, shigella and staphylococcus aureus, and the reagent comprises specific primers and Taqman probes corresponding to the four food-borne pathogenic bacteria. The quadruple Taqman qPCR reagent provided by the invention has the characteristics of high efficiency, accuracy, sensitivity, rapidness and the like, provides an accurate, rapid, efficient and economical means for detecting the four food-borne pathogenic bacteria in the quality safety detection work of aquatic products and processed products thereof, and has important significance for guaranteeing the edible safety of the aquatic products in China.

The invention discloses a Taq DNA polymerase mutant with improved excision activity and application thereof, and belongs to the technical field of enzyme engineering. The invention aims to solve the technical problems of low probe cutting efficiency, weak fluorescence signal, limited detection sensitivity (especially for low-abundance targets), low reaction speed (high Ct value) and reduced specificity caused by insufficient 5 '-> 3' excision enzyme activity of wild Taq DNA polymerase in TaqMan probe method qPCR (quantitative polymerase chain reaction). According to the technical scheme, the Taq DNA polymerase mutant with the high 5 '-3' exonuclease activity is provided, the amino acid sequence of the Taq DNA polymerase mutant is shown as SEQ ID NO.2, the exonuclease activity of the mutant is improved by 77%, the cutting speed of a TaqMan probe is greatly increased, accumulation of fluorescence signals is accelerated, the Ct value is reduced, and the detection time is remarkably shortened.

Disclosed are a probe method-based kit and method for tracking a behavior trajectory of Acipenser dabryanus Dumeril released upstream of the Yangtze River. By analyzing mitochondrial DNA sequences of Acipenser dabryanus Dumeril and common fish species in the Yangtze River basin, a pair of specific primers ND5-F4 / ND5-R4 capable of specifically amplifying mitochondrial DNAs of Acipenser dabryanus Dumeril and a Taqman fluorescent probe ND5 Probe 4 are designed and screened. A kit composed of the specific primers, the Taqman fluorescent probe, and a ddPCR master mix is used to carry out ddPCR testing on environmental DNA samples collected before and after release, so that a behavior trajectory of the released Acipenser dabryanus Dumeril can be tracked and monitored, achieving "non-contact" tracking of the behavior trajectory of the released Acipenser dabryanus Dumeril, avoiding damage to fish bodies in the tracking process, leading to reduced tracking costs and improved accuracy of overall tracking.

The invention discloses a detection kit and method for Mycobacterium tuberculosis complex and rifampicin drug-resistant mutation, and belongs to the technical field of biologication.A Taqman probe and a molecular beacon are combined, a fluorescent signal is generated based on a Taq enzyme hydrolysis fluorescent probe, the fluorescent signal is accumulated, and the molecular beacon is used for detecting the drug-resistant mutation of the Mycobacterium tuberculosis complex and the rifampicin drug-resistant mutation of the Mycobacterium tuberculosis complex and the rifampicin drug-resistant mutation. According to two technical principles that a molecular beacon and a fluorescent product are hybridized to generate a fluorescent signal, fluorescent PCR amplification is adopted for detection of one target spot in a monochromatic fluorescent channel, and a melting curve mode of a probe and a target gene is adopted for detection of the other target spot. Therefore, two target spots can be detected and analyzed in a monochromatic fluorescence channel at the same time. Single-tube multiple detection is achieved, and mycobacterium tuberculosis complex group detection and mycobacterium tuberculosis rifampicin drug-resistant mutation site detection can be achieved at the same time.

The invention discloses a primer and probe composition for detecting nocardia seriolae based on TaqMan qPCR (quantitative polymerase chain reaction) and application of the primer and probe composition. According to the invention, a qualitative and quantitative detection method for nocardia seriolae is established by utilizing the specific primers and probes, and the method is used for carrying out qualitative detection on water bodies and fish bodies of farms of snakeheads and hybrid snakeheads in different regions and detecting different tissues after the hybrid snakeheads counteract the nocardia seriolae, and has a very good detection effect. According to the invention, the purposes of nocardia seriolae monitoring, nocardia disease diagnosis, pathological degree measurement and the like can be realized, a reliable detection tool can be provided for nocardia seriolae epidemiological investigation, and nocardia disease can be diagnosed in an early stage so as to guide culture management and reasonable medication treatment; and a research basis and a technical support are provided for exploring epidemic characteristics and pathological mechanisms of nocardia seriolae.

The embodiment of the invention discloses a fluorescent primer combination for identifying TaqMan real-time fluorescent quantitative PCR of trachemys scripta elegans, a kit and application. Comprising a probe, an upstream primer and a downstream primer, the nucleotide sequence of the probe is 5 '-FAM-TTACTCCGACTATCCAG-MGB-3', the nucleotide sequence of the upstream primer is 5 '-TCCTAGGCCTTGCTGGAATG-3', and the nucleotide sequence of the downstream primer is 5 '-TGAAATAGAATTCTATGCG-3'. The TaqMan real-time fluorescent quantitative PCR probe primer combination developed by the invention has the characteristics of strong specificity and high sensitivity in the aspect of identifying trachemys scripta elegans species, and the detection accuracy and reliability are greatly improved; powerful technical support is provided for effectively monitoring distribution and diffusion of trachemys scripta elegans, preventing and controlling damage of trachemys scripta elegans to the ecological environment and guaranteeing public health safety.

The invention discloses a double Taqman detection probe primer group for trichina and sarcocystis and application of the double Taqman detection probe primer group. Relates to the technical field of parasite detection. The invention provides a detection probe primer group, a kit and application. According to the detection method established by the invention, an amplification curve has a good linear relationship; the sensitivity is high; the specificity is high; dNA detection on 12 parasites such as toxoplasma gondii, ascaris suum, cryptosporidium and pulmonary nematode does not have an amplification curve; agarose gel electrophoresis shows that the molecular mass of the obtained PCR product strip accords with expectation. The kit has the characteristics of simplicity in operation, high specificity and high sensitivity, can quickly and efficiently detect trichina and sarcocystis, and has guiding significance in quick laboratory diagnosis of infection of the trichina and sarcocystis.

The invention relates to a primer probe combination and a kit for detecting eDNA of Chinese sturgeons and application of the primer probe combination and the kit. Specific primers and TaqMan probes are designed for a D-loop region of Chinese sturgeon mitochondrial DNA, and efficient specific amplification and absolute quantification of Chinese sturgeon eDNA in an environmental sample are realized by optimizing a reaction system and amplification conditions. Compared with the prior art, the method is simple and convenient to operate, does not need to perform harmful sampling on Chinese sturgeon individuals, is suitable for resource monitoring and protection evaluation in a large-range and dynamic environment, and has high sensitivity, high accuracy and high practical value.

The invention discloses a primer and probe combination for detecting a bulbus fritillariae cirrhosae medicinal material based on Taqman fluorescent probe double-marking linear correction quantitative PCR (Polymerase Chain Reaction). The invention further discloses a method for detecting the bulbus fritillariae cirrhosae medicinal material based on Taqman fluorescent probe double-marking linear correction quantitative PCR, the bulbus fritillariae cirrhosae specific primers and probes designed based on six bulbus fritillariae cirrhosae-based original plant conserved gene sequences and the 18sRNA universal reference gene primers and probes are adopted, a sample to be tested and a standard reference substance are amplified at the same time in a PCR reaction system, and the specific primers and probes are used for detecting the specific genes of the bulbus fritillariae cirrhosae medicinal material based on Taqman fluorescent probe double-marking linear correction quantitative PCR. The method comprises the following steps: qualitatively confirming that a test sample contains the bulbus fritillariae cirrhosae component according to amplification results of a bulbus fritillariae cirrhosae specific primer and a probe, then taking a standard reference substance as a template to obtain two standard curves of a bulbus fritillariae cirrhosae specific gene and a 18sRNA gene, and finally quantitatively analyzing the bulbus fritillariae cirrhosae content in the test sample by adopting a double-marking linear correction method. The method can be used for efficiently, specifically and accurately detecting the ingredients and content of the bulbus fritillariae cirrhosae through PCR.

The invention discloses a probe primer combination and a kit for rapidly detecting five kinds of diarrheogenic Escherichia coli. The method comprises the following steps: respectively designing a group of specific primers and Taqman probes according to escV, stx1, stx2 and bfp genes of EPEC and STEC / EHEC, an ipaH gene of EIEC, lt, stp and sth genes of ETEC, and aggR, pic and astA genes of EAEC; the kit can be used for detecting various diarrheogenic Escherichia coli in a single tube at the same time, or can be used for detecting five kinds of diarrheogenic Escherichia coli in different reaction tubes within 30 minutes at the soonest, can meet higher sensitivity, and has definite clinical significance and potential application value.

The invention relates to the technical field of typing identification, in particular to a primer and probe combination for typing identification of mycobacterium abscessus subspecies and a method and application of the primer and probe combination. The primer and probe combination comprises three subspecies specific amplification primer pairs and three subspecies specific TaqMan fluorescent probes; the sequences of the three pairs of subspecies specific amplification primers are SEQ ID NO. 1 to SEQ ID NO. 6; wherein the TaqMan fluorescent probe comprises three TaqMan fluorescent probes corresponding to mycobacterium abscessus subtypes, two ends of each TaqMan fluorescent probe are respectively provided with a fluorophore and a quenching group, and each sequence is SEQ ID NO.7 to SEQ ID NO.9. The invention further discloses a kit with the PCR reaction liquid, and by means of the PCR reaction liquid, the mycobacterium abscessus can be rapidly and accurately detected and identified, and subspecies typing identification can be conducted on the mycobacterium abscessus.

The invention provides a kit for detecting human BRAF gene V600E and TERT gene promoter mutation, and belongs to the technical field of biology. The kit comprises a primer, a probe, a 5 * PCR (Polymerase Chain Reaction)-Enhancer, a 10 * PCR Buffer, dNTP (Deoxyribonucleoside Triphosphate) Mix, MalTaq DNAPlyase, magnesium ions, DEPC (Diethylpyrocarbonate) water, a positive control and a negative control. According to the present invention, the ARMS-PCR detection technology is adopted to detect the specific gene mutation, and the target mutation site is identified by designing the specific primer and the probe, such that the detection specificity and the detection sensitivity are improved; by combining with a Taqman probe technology, the fluorescent signal change in the PCR amplification process can be monitored in real time, so that whether mutation of a BRAF V600E site, a TERT C228T site and a TERT C250T site exists or not is accurately judged; according to the method, pre-amplification of 15 cycles is adopted, then quantitative amplification of 30 cycles is adopted, and when the initial amount of the target DNA is very low, the copy number of the target DNA can be increased, so that the detection accuracy and sensitivity are further improved.

The invention discloses a PCR (polymerase chain reaction) detection primer, a probe and a detection kit for identifying PRRSV (porcine reproductive and respiratory syndrome virus). According to the method, specific primers and probes are designed and screened for a PRRSV conserved gene ORF6, the primers are composed of two nucleotide sequences as shown in SEQ ID No.1-2, and the sequence of the probes is as shown in SEQ ID No.3; the invention further discloses a TaqMan fluorescent quantitative PCR detection kit for identifying the porcine reproductive and respiratory syndrome virus. The TaqMan fluorescent quantitative PCR detection kit comprises a specific primer, a TaqMan probe and a positive control. The specific primer and the probe provided by the invention can be used for rapidly and accurately identifying the PRRSV, have good sensitivity and specificity, effectively overcome the defect that the existing detection method is insufficient in detection coverage on a new variant or a recombinant strain of the PRRSV, and reduce the possibility of false negative leak detection.

The invention discloses TaqMan real-time fluorescent quantitative PCR (polymerase chain reaction) primers, a probe, a detection method and a kit for detecting oviductus ovatus and application of the TaqMan real-time fluorescent quantitative PCR primers and the probe. The primer comprises an upstream primer HO-F and a downstream primer HO-R, and the nucleotide sequences of the upstream primer HO-F and the downstream primer HO-R are respectively shown as SEQ ID NO. 1 and SEQ ID NO. 2. The nucleotide sequence of the probe HO-P is shown as SEQ ID NO.3, the 5'end of the probe HO-P is marked with a fluorescence reporter group FAM, and the 3 'end of the probe HO-P is marked with a quenching group BHQ1. The TaqMan real-time fluorescent quantitative PCR method established by using the primer and the probe can specifically detect the ovulus ovatus of sheep, and has no cross reaction with other bacteria of ovulus ovatus and 47 common bacteria of cattle and sheep. The method or the kit not only can be applied to rapid identification of oviductus ovatus, but also can be applied to qualitative, relative quantitative and absolute quantitative detection of oviductus ovatus in cattle and sheep clinical samples, has the advantages of strong specificity, high sensitivity, good repeatability and the like, provides a reliable method for rapid detection of oviductus ovatus infection of cattle, sheep and sheep, and has a wide application prospect. Good popularization and application prospects are realized.

The application relates to a primer probe composition, a kit and a detection method for specifically detecting Blattella germanica. The specific primer and TaqMan probe are designed and screened based on the specific sequence of the mitochondrial COI gene of Blattella germanica. The detection method has extremely high specificity, has no cross reaction with close species such as Periplaneta americana and Blattella germanica, has high sensitivity, the minimum detection limit is 1 fg / muL, and has good repeatability, with the variation coefficients of groups and between groups being less than 3%.

The invention belongs to the technical field of biological detection, and particularly relates to a primer probe pair, a microfluidic chip and a kit for detecting pathogens of vector infectious diseases based on a TaqMan microfluidic chip technology as well as a preparation method and application of the primer probe pair. Based on a TaqMan microfluidic chip technology, the invention provides a detection method for simultaneously detecting 28 kinds of pathogens of the disease-borne infectious diseases, and the related pathogens of the disease-borne infectious diseases comprise viruses, bacteria and parasites, and are wide in range and large in difference. The detection method provided by the invention has a low detection limit (5-20 copies / [mu] L); when the method is applied to an actual sample, the sensitivity, specificity, accuracy and precision are high, and compared with a gold standard method, the consistency is good; and compared with a traditional qPCR method, the method has the advantages that the detection time efficiency and the manpower and material consumption are greatly reduced, and the method is particularly suitable for disease monitoring and emergency disposal scenes with undefined pathogen spectrum, need of rapid and comprehensive troubleshooting and the like, and has a good application prospect.

The invention discloses a primer and Taqman probe combination for detecting pathogenic tick-borne viruses and a detection method, and the primer and Taqman probe combination for detecting the pathogenic tick-borne viruses comprises the following steps: (1) detecting six viruses such as an Alongshan virus (ALSV), a forest encephalitis virus (TBEV), a severe fever with thrombocytopenia syndrome virus (SFTSV), a Songling virus (SGLV), a BJNV and a Yezo virus (Yezo virus, YEZV) and the like, and (2) detecting the six viruses such as the Alongshan virus, the TBEV, the SFTSV, the Songling virus, the SGLV, the BJNV and the Yezo virus. Each primer pair comprises an upstream primer F, a downstream primer R and a Taqman probe. According to the application, common pathogenic virus ALSV, TBEV, SFTSV, SGLV, BJNV and YEZV virus seeds in northeast China are respectively detected by using Taqman probe detection through fluorescent quantitative PCR, a rapid and accurate virus diagnosis and detection method is developed, and viral tick-borne diseases are scientifically prevented and treated.

The invention relates to a quadruple TaqMan real-time fluorescent quantitative PCR (Polymerase Chain Reaction) primer group and a probe group for simultaneously detecting cGPV (Complementary Glutathione Virus), MDPV (Minimal Disease Papilloma Virus), MDGPV (Minimal Disease Papilloma Virus) and SBDSV (Sequence Broadcast DSV) and a kit thereof. The sequences of the primer group and the probe group are respectively shown as SEQ ID NO.1-12. The research successfully develops and verifies a multiple TaqMan-MGB real-time fluorescent PCR method capable of simultaneously detecting and distinguishing four important waterfowl parvoviruses (cGPV, MDPV, MDGPV and SBDSV). The method has good specificity, repeatability and high sensitivity. Compared with a conventional PCR method, the multiple detection method has the advantages that the clinical waterfowl parvovirus detection rate is remarkably improved, mixed infection can be effectively recognized, and the clinical diagnosis efficiency is greatly improved.

The invention discloses an aptamer-based oxytetracycline fluorescent quantitative PCR (Polymerase Chain Reaction) detection kit as well as a preparation method and a detection method thereof, and relates to the technical field of analysis and detection.The detection method comprises the following steps: mixing and incubating a sample to be detected, an oxytetracycline specific aptamer, a complementary chain and a magnetic bead curing capture probe; magnetically separating out the mixed and incubated magnetic beads, curing and capturing the probe, and washing to obtain a magnetic compound; adding PCR reaction liquid containing the primer pair and a TaqMan probe into the magnetic compound, carrying out fluorescent quantitative PCR amplification, and monitoring a fluorescent signal in real time to obtain a Ct value; establishing a standard curve between the oxytetracycline concentration and the Ct value of the fluorescent quantitative PCR, and determining the oxytetracycline concentration in the sample to be detected according to the standard curve. Based on an indirect competitive mechanism, through signal conversion design, combination of oxytetracycline and the aptamer is converted into quantitative analysis of free sDNA, and through integration of three technologies of aptamer free recognition, magnetic bead targeted capture and qPCR signal amplification, the sensitivity and anti-interference capability of oxytetracycline detection are improved.

The invention discloses a specific molecular target of shigella flexneri and a detection method of the specific molecular target. The nucleotide sequence of the specific molecular target is as shown in SEQ ID NO. 1. The nucleotide sequences of an upstream primer of the specific molecular target SEQ ID NO.1 are as shown in SEQ ID NO.2 and SEQ ID NO.5, and the nucleotide sequences of a downstream primer of the specific molecular target SEQ ID NO.1 are as shown in SEQ ID NO.3 and SEQ ID NO.6. And the nucleotide sequences of the TaqMan probes are as shown in SEQ ID NO. 4 and SEQ ID NO. 7. According to the invention, the detection sensitivity of the shigella flexneri can reach 3.9 * 10 < 2 > CFU / mL, meanwhile, the method has better specificity and repeatability, and a simple, convenient, rapid, efficient and low-cost method is provided for the detection of the shigella flexneri.

The invention discloses a primer, a TaqMan probe and a kit for carrying out fluorescent PCR (Polymerase Chain Reaction) detection on bovine-derived ureaplasma, and belongs to veterinary detection of animal epidemic diseases in the technical field of biological detection. The invention discloses a primer and a TaqMan probe which are used for carrying out fluorescent PCR (Polymerase Chain Reaction) detection on bovine-derived ureaplasma, and the primer and the TaqMan probe are an upstream primer U.diversum-F3, a downstream primer U.diversum-R3 and a TaqMan probe U.diversum-P3. The kit is simple and convenient in detection operation, strong in specificity, high in sensitivity and good in repeatability, and can be used for qualitatively detecting the bovine-derived ureaplasma; technical support is provided for detection of bovine-derived ureaplasma, epidemic situation epidemiological investigation, epidemic disease monitoring and purification, strain screening and quality monitoring and stable production and supply conservation in the vaccine production process, and the method plays an important role.

The invention belongs to the technical field of biology, and discloses a TaqMan primer probe combination for detecting virulent and attenuated Newcastle disease, which comprises a virulent primer RT, a virulent probe RT-probe, a universal primer SRT and a universal probe SRT-probe, the virulent primer RT comprises an upstream primer RT-F and a downstream primer RT-R, and the universal primer SRT comprises an upstream primer SRT-F and a downstream primer SRT-R; a TaqMan probe fluorescent quantitative RT-PCR (Reverse Transcription-Polymerase Chain Reaction) detection method for virulent and attenuated NDV (Newcastle Disease Virus) established by using the TaqMan primer probe combination has no cross reaction on amplification of common avian viruses AIV, FADV, IBV and IBDV and has good specificity; the lowest detection concentration of virulent and attenuated Newcastle disease virus is 101 copies / uL, which is 100 times higher than that of a conventional RT-PCR method, and the kit has good sensitivity; and compared with the conventional RT-PCR method, the detection rate is 100% and is superior to that of the conventional RT-PCR method, and the detection result is consistent with the sequencing result.