Kit and method for detecting polymorphism of CYP2C19 gene

A technology of CYP2C19 and gene polymorphism, applied in the field of fluorescent quantitative PCR, can solve the problems of low sensitivity and specificity of polymorphism, low sensitivity and specificity, long detection time, etc., to eliminate false positives and false negatives , avoid false positive or false negative, high accuracy effect
CN103468818AActive Publication Date: 2013-12-25刘辉
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Patent Information

Authority / Receiving Office
CN · China
Current Assignee / Owner
刘辉
Publication Date
2013-12-25

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Abstract

The invention relates to a kit and a method for detecting polymorphism of CYP2C19 gene, and belongs to the field of fluorogenic quantitive PCR (Polymerase Chain Reaction). The kit comprises a detection primer and a fluorescent probe, which comprise at least one group of specific primer and specific Taqman fluorescent probe of CYP2C19 gene CYP2C19*2 polymorphism, specific primer and specific Taqman fluorescent probe of CYP2C19 gene CYP2C19*3 polymorphism, and specific primer and specific Taqman fluorescent probe of CYP2C19 gene CYP2C19*17 polymorphism. The kit is adopted for detecting the CYP2C19 gene, the sensitivity and specificity are both obviously improved, the detection time is short and the predication of medicament dosage is facilitated.
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Description

technical field

[0001] The invention relates to the field of fluorescent quantitative PCR, in particular to a kit and method for detecting CYP2C19 gene polymorphism. Background technique

[0002] The CYP2C19 gene is an important drug-metabolizing enzyme that plays a key role in drug response. This is because there are significant individual differences in the activity of the CYP2C19 gene, manifested as genetic polymorphisms, resulting in individual differences in blood drug concentrations , the study of its genetic polymorphism will provide guidance for clinically determining the optimal therapeutic dose, formulating a rational drug regimen, maximizing the curative effect, avoiding and reducing adverse reactions.

[0003] In the CYP2C19 gene, the distribution frequencies of CYP2C19*2 polymorphism and CYP2C19*3 polymorphism in the Chinese population are 39.9% and 6.1%, respectively, which lead to the loss of activity of the protein encoded by CYP2C19, which leads to drug meta...

Claims

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