156 results about "CYP2C19" patented technology

The invention relates to a medicine metabolic relevant loci detection method, which comprises the following steps: extracting genome DNA from human samples; respectively designing a Taqman probe pair and a primer pair according to at least two medicine metabolic relevant genes; respectively marking the 5' end and the 3'end of the Taqman probe pair with fluorescence reporting genes and fluorescence quenching genes; carrying out fluorescence quantitative PCR augmentation on the genome DNA; and judging whether the medicine metabolic relevant genes have the mutation according to the fluorescence quantitative PCR augmentation results. Preferably, the number of the medicine metabolic relevant genes is four, the Taqman probe pair and the primer pair are used for detecting a loci rs1057910 of a gene CYP2C9, a loci rs4244285 of a gene CYP2C19, a loci rs4986893of a gene CYP2C19, a loci rs1065852 of a gene CYP2D6 and a loci rs28371759 of a gene CYP3A4. The invention has the advantages of ingenious design, simple operation and accurate and reliable detection results, and provides the reference frame for determining whether professional doctors are needed to be consulted so as to make sure the medicine can be taken or not or the proper dosage and the like when a certain medicine is taken.

The invention provides a novel method for detection of human CYP2C19 gene polymorphism. The method comprises the following steps: (1) an ARMS primer is designed. The 3' end of the ARMS primer is a mutation site, and any missense in first-third nucleotide positioned upstream of a mutation site is mutated; (2) after allelic ARMS primer is designed for each SNP, an artificial nucleotide sequence with no homology with human gene sequence is respectively introduced into the 5' end of each of the two specific ARMS primers; (3) according to the introduced general artificial sequence, a Taqman probe which is completely complementary and paired up with the intermediate sequence fragment of the general sequence is designed, and a specific fragment amplified by the ARMS primer is specifically identified. By the above detection method and the kit, the capability of the allelic specific primer in distinguishing different alleles can be greatly enhanced; and accuracy of detection results is guaranteed, cost is greatly saved and efficiency is raised.

The invention relates to the technical field of DNA detection and provides an SNP typing method and kit. A to-be-tested SNP site comprises SNP sites in CYP2C9, VKORC1 and CYP2C19 genes. The SNP typing method comprises the following steps: replacing a fluorescently-labeled oligonucleotide probe with a non-fluorescently-labeled oligonucleotide probe, performing one-time connection sequencing reaction on to-be-sequenced library molecules and then obtaining sequence information of the to-be-tested SNP site through connection sequencing. The SNP typing kit comprises a CYP2C9 specific primer pair, a VKORC1 specific primer pair, a CYP2C19 specific primer pair and the non-fluorescently-labeled oligonucleotide probe. The SNP typing method and kit provided by the invention have the benefit that through sealing non-specific binding sites in the to-be-sequenced library molecules, a false signal in a result obtained by the connection sequencing is reduced, so that the accuracy of SNP site detection is improved.

The invention discloses a quick detection kit for the CYP2C19*2 genetype. The quick detection kit comprises a PCR mixed solution, wherein the PCR mixed solution comprises the following raw materials: DNA (deoxyribonucleic acid) polymerases, DNTPs, CYP2C19*2 forward primers, CYP2C19*2 reverse primers, CYP2C19*2 mutant molecular beacons, CYP2C19*2 wild-type molecular beacons, MgC12, 5x Colorless Reaction Buffer and a cell lysis solution. The quick detection kit overcomes the defects that the conventional kit aiming at clinically detecting the CYP2C19*2 genetype and the detection method thereof cannot satisfy quick diagnosis of instructing clinical medication, and the quick detection kit and the detection method thereof provided by the invention can quickly detect the CYP2C19*2 genetype, and reduces patients' medical treatment risk.