Pair of specific primers and probe for detection of CYP2C19 gene chip

A CYP2C19, specific technology, applied in the field of molecular biology, can solve the problems of inaccurate detection results, long detection cycle, and difficulty in meeting clinical tests.
CN103525925AInactive Publication Date: 2014-01-22SHANGHAI BAIO TECH
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Patent Information

Authority / Receiving Office
CN · China
Current Assignee / Owner
SHANGHAI BAIO TECH
Publication Date
2014-01-22
Estimated Expiration
Not applicable · inactive patent

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Abstract

The invention relates to the molecular biology field, and discloses a specific oligonucleotide probe for detection of SNP loci of rs4244285681G / A and rs4986893636G / A of a CYP2C19 gene and a pair of specific primers for amplification of the CYP2C19 gene during detection of the above SNP loci. The probe can hybridize with different genotypes of the CYP2C19 gene specifically, and the genotypes comprise a CYP2C19*1 type, a CYP2C19*2 type and CYP2C19*3 type. The products can be used for detection of metabolic abilities for relevant medicines (such as plavix, omeprazole, voriconazole and the like) and of Chinese population, clinical medication schemes can be guided and adjusted, clinical personalized medication can be provided with a basis, the curative effects can be raised and the risks of toxic and side effects can be reduced.
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Description

technical field

[0001] The invention relates to the fields of molecular biology and nucleic acid detection, specifically designing specific primer pairs for amplifying CYP2C19 genes and specific probes for CYP2C19 gene chip detection. Background technique

[0002] Cytochrome P450 isozymes, also known as drug enzymes, are a superfamily composed of a series of structurally and functionally related enzymes, and are the main enzyme system for drug metabolism in the human body. The composition of P450 enzyme system is complex and controlled by gene diversity. At least 12 subfamilies are known. Many P450 isoenzymes have genetic polymorphisms, which make the corresponding enzyme activities show differences, and the ability to metabolize drugs is also different. According to the differences in drug metabolism ability, the population is divided into four types: ultrafast metabolizers (UM), fast metabolizers (EM), intermediate metabolizers (IM), and slow metabolizers (PM).

[0003] ...

Claims

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