234 results about "Biology (field)" patented technology

The invention relates to a composition for identifying epinephelus fuscoguttatus, epinephelus coioides and hybrid offspring and application, and belongs to the field of molecular biology, the composition comprises a pair of primers and a group of probes, and the sequences are shown as SEQ ID NO.1-4. The invention further provides a kit for identifying the epinephelus fuscoguttatus, the epinephelus coioides and the hybrid offspring thereof, and the kit is used for identifying the epinephelus fuscoguttatus and the hybrid offspring of the epinephelus coioides and the hybrid offspring of the epinephelus coioides and the hybrid offspring of the epinephelus coioides. The kit comprises the composition disclosed by the invention. The composition can be used for rapidly identifying epinephelus fuscoguttatus, epinephelus coioides and hybrid filial generation gold and tiger hybrid spots of the epinephelus coioides, the epinephelus coioides and the hybrid filial generation gold and tiger hybrid spots of the epinephelus coioides and the epinephelus coioides and the hybrid filial generation gold and tiger hybrid spots of the epinephelus

The invention belongs to the field of molecular biology, and particularly relates to a transgenic soybean event LP207-1 and a detection method thereof. The invention provides a nucleic acid sequence for detecting a transgenic soybean event LP207-1, the nucleic acid sequence is selected from one or more of sequences SEQ ID NO: 1-7 or complementary sequences thereof, and the nucleic acid sequence is derived from a plant, a seed or a cell of the transgenic soybean event LP207-1. A representative sample of a seed of the transgenic soybean event LP207-1 is preserved in the China Center for Type Culture Collection on May 23, 2025, and the preservation number is CCTCC NO: P202516. The transgenic soybean event LP207-1 has good resistance to lepidoptera pests and can tolerate agricultural herbicides containing glyphosate and glufosinate-ammonium, economic loss caused by the lepidoptera pests can be avoided, and the yield is not reduced; and the breeding efficiency is enhanced, and the molecular marker can be used for tracking a breeding population and transgenic insertion fragments in offspring of the breeding population. The detection method provided by the invention can quickly, accurately and stably identify the existence of the plant material derived from the transgenic soybean event LP207-1.

The invention relates to a river ecosystem planktonic bacteria environment threshold identification method, and belongs to the field of environmental ecology and computational biology. The method comprises the following steps: collecting a plurality of environmental factors, fusing and constructing a space-time aligned watershed ecosystem data set, and preprocessing the data set; constructing a prediction model and performing training; environmental factors are collected from a target drainage basin and input into the trained prediction model, and a nonlinear mapping relation between the environmental factors and the microbial community, namely an SHAP value, is obtained through learning; constructing a GAM response curve based on the SHAP values and the environmental factors, fitting a nonlinear response trend of the GAM response curve through a spline function, and identifying an environmental threshold corresponding to each environmental factor; through a Bootstrap resampling method, multiple times of environment threshold recognition are carried out on each environment factor, and a confidence interval is estimated based on environment thresholds obtained through multiple times of recognition, so that the stability and reliability of environment threshold recognition are improved.

The invention belongs to the field of molecular biology, and discloses acquisition of a waterlogging-resistant major QTL qWT1.1 in a corn seedling stage and development and application of a molecular marker primer of the waterlogging-resistant major QTL qWT1.1. The invention provides a major QTL qWT1.1 for controlling the waterlogging survival rate in the corn seedling stage, the major QTL qWT1.1 is located at the fifth version physical position of a reference genome in the first staining of the corn, the fifth version physical position of the reference genome is Chr1: 25904794-27039204, the major QTL site is used for controlling waterlogging-resistant phenotypic variation, an excellent haplotype SNP marker closely linked with the major QTL site is located at the 27039148th basic group of a first chromosome of a reference genome of the corn B73, and the excellent haplotype SNP marker is located at the 27039148th basic group of a second chromosome of the reference genome of the corn B73. The explained waterlogging survival rate phenotypic contribution rate is 10.03%, the PARMS marker designed by the SNP is used for detecting a corn inbred line group, the operation is simple and convenient, the typing is clear, the good selection effect on characters such as root length, root number, plant height, branch number and yield under the stress of corn waterlogging is realized, and the application prospect is wide. The molecular marker can be used for molecular marker-assisted selective breeding of the waterlogging resistance character in the corn seedling stage.

The invention belongs to the field of molecular biology, and particularly relates to application of OsDOF3 protein or a coding gene thereof to improvement of rice broad-spectrum disease resistance and regulation of agronomic traits. According to the application, an OsDOF3 knockout mutant and an overexpression transgenic line are constructed under the background of susceptible rice, resistance phenotype analysis is performed by inoculating various pathogenic bacteria (including pyricularia oryzae, rhizoctonia solani and bacterial blight), and it is proved that OsDOF3 participates in regulation and control of an immunoreaction pathway of rice to fungal diseases (rice blast and sheath blight) and bacterial diseases (bacterial blight). Meanwhile, the application also finds that after a strong promoter ubi is adopted for driving, an overexpression material of the OsDOF3 is shorter than that of a wild plant, and the OsDOF3 influences normal growth and development of rice while stimulating plant immune response. The invention provides important gene resources and molecular theoretical basis for developing a new variety of broad-spectrum disease-resistant rice.

The invention discloses osmanthus transcription factors OfbHLH10 and OfWRKY78 for regulating synthesis of a flower fragrance component beta-ionone as well as a coding gene and application of the osmanthus transcription factors OfbHLH10 and OfWRKY78, and belongs to the field of plant molecular biology. The amino acid sequence of the osmanthus fragrans transcription factor OfbHLH10 provided by the invention is as shown in SEQ ID NO. 2, and the amino acid sequence of the transcription factor OfWRKY78 interacting with the osmanthus fragrans transcription factor OfbHLH10 is as shown in SEQ ID NO. 4. According to the invention, carriers constructed by CDs of OfWRKY78 and a promoter of OfCCD4 are jointly injected into Ben's tobacco leaves, a dual-luciferase experiment is carried out, and a result shows that OfbHLH10 has a promoting effect on expression of a synthetase gene OfCCD4 of beta-ionone, OfWRKY78 has an inhibiting effect on expression of OfCCD4, and OfbHLH10 and OfWRKY78 interact with each other and has an antagonistic effect on expression of OfCCD4. The gene provided by the invention can effectively regulate and control the content of beta-ionone in osmanthus fragrans fragrance, and has a good application prospect.

The invention relates to a penaeus monodon high temperature resistance character related SNP marker primer based on whole genome association analysis and application, and belongs to the field of molecular biology. The nucleotide sequence of the primer pair is shown in SEQ ID NO.1-2. An SNP molecular marker is located at the 27494780 site of the 27th chromosome of a penaeus monodon genome and is shown as Cgt; a mutation, and the A mutation is associated with a high temperature resistant character. The invention further provides a kit for detecting the high-temperature-resistant character of penaeus monodon, and high-temperature-resistant individuals can be screened by utilizing the primers and are used for breeding new varieties.

The invention relates to the field of plant molecular biology, in particular to a gene for controlling stem diameter and height of a tomato plant and application of the gene, the gene is an MYC1 gene, and the MYC1 gene is a nucleotide sequence capable of coding the following protein (a) or (b): (a) a protein composed of an amino acid sequence shown in SEQ ID NO: 2; and (b) a protein which is derived from (a) and has the same enzyme activity, wherein one or more amino acid sequences are substituted, deleted or added into the amino acid sequence in (a). The gene can control the stem diameter and height of a tomato plant, and by increasing the expression quantity of the MYC1 gene in the tomato plant, the stem of the tomato plant can be thickened, the internode spacing of the tomato plant can be shortened, and the tomato plant can be shortened. Furthermore, the stem diameters and heights of different plants and the subsequent cultivation of varieties with different stem diameters and heights can be controlled through a subsequent cloning technology.

The invention discloses a CAPS molecular marker of a GhFRO7 gene of upland cotton and application of the CAPS molecular marker in early blossoming character identification and breeding, and belongs to the field of molecular biology. Whole genome association analysis is carried out based on a high-density SNP marker, an SNP site which is located on an upland cotton GhFRO7 gene and is remarkably related to the flowering time character is identified and obtained, and then a CAPS molecular marker with the sequence shown as SEQ ID NO.34 and a primer pair with the sequence shown as SEQ ID NO.3-4 are developed. The CAPS molecular marker and the primer pair thereof provided by the invention can be applied to identification of early flowering upland cotton varieties and molecular marker-assisted selective breeding, and can effectively save the detection cost, shorten the period and improve the detection efficiency. The invention provides a new method for genetic improvement of cotton prematurity, and provides important genetic resources for developing cotton varieties adapting to specific climate conditions.

The invention discloses a KASP molecular marker related to gibberellic disease resistance on a wheat 7B chromosome and application of the KASP molecular marker, and belongs to the field of molecular biology. The KASP molecular marker closely associated with wheat scab resistance is screened, and the nucleotide sequence of the KASP molecular marker is as shown in SEQ ID NO.1; a primer group for amplifying the KASP molecular marker is further designed, and the primer group comprises upstream primers F1 and F2 and a downstream primer R which are connected with fluorescent connectors with different colors. Experimental results show that the KASP molecular marker developed by the invention can be used for efficiently and accurately identifying whether the wheat to be detected has gibberellic disease resistance or not so as to separate gibberellic disease resistant single plants in a population for improved breeding of the wheat, so that the detection cost can be effectively saved, the period can be shortened, and the breeding efficiency can be improved. The invention provides a new theoretical basis and technical means for disease-resistant breeding of wheat, and has outstanding industrial application value.

The invention discloses a transcription factor OfMYB187 for regulating synthesis of beta-ionone of a sweet osmanthus fragrance component, an interaction protein OfCLpP of the transcription factor OfMYB187 as well as coding genes and application of the transcription factor OfMYB187 and the interaction protein OfCLpP, and belongs to the field of plant molecular biology. The amino acid sequence of the transcription factor OfMYB187 provided by the invention is as shown in SEQ ID NO. 2, and the amino acid sequence of the interaction protein OfCLpP of the transcription factor OfMYB187 is as shown in SEQ ID NO. 4. According to the application disclosed by the invention, a carrier constructed by promoters of OfMYB187, OfCLpP and OfCCD4 is jointly injected into a Ben's tobacco leaf, firefly and renilla determination is carried out, and a result shows that the OfMYB187 has an inhibition effect on a synthetase gene OfCCD4 of beta-ionone, but after interaction of the OfMYB187 and the OfCLpP, the OfMYB187 protein can be hydrolyzed, and the inhibition effect of the OfMYB187 protein on the OfCCD4 is relieved. The gene provided by the invention can directly or indirectly regulate and control the content of beta-ionone in osmanthus fragrans fragrance, and has a good application prospect.

The invention relates to a method for identifying Langya chicken and Non-Langya chicken by using SNP molecular markers, and belongs to the technical fields of animal husbandry and molecular biology. The SNP molecular marker for Langya chicken identification comprises 12 loci. The invention further provides a primer combination which comprises SNP loci for amplifying Langya chicken identification. According to the method, the 12 specific SNP sites of the Langya chicken are utilized, the site information is obtained through PCR amplification and Sanger sequencing, prediction is conducted through machine learning, the Langya chicken variety identification model is established based on the 12 SNP site combination, and the method has the advantages of being low in cost, simple in sequencing method, high in speed and high in accuracy.

The present invention relates generally to the field of molecular biology and concerns a method for high throughput screening and characterization of one or more candidate plant pathogen effect genes and / or one or more candidate plant disease resistance genes in a single plant cell, the method comprises: (i) introducing into a plant protoplast one or more expression cassettes comprising at least one candidate plant pathogen effect gene and / or at least one candidate plant disease resistance gene; (ii) transiently expressing the one or more candidate plant pathogen effect genes and / or the one or more candidate plant disease resistance genes; (iii) measuring reactive oxygen species (ROS) produced by the protoplast; then (iv) separating the plant protoplast producing the ROS; and (v) identifying the introduced at least one candidate plant pathogen effect gene and / or at least one candidate plant disease resistance gene. Alternatively, the protoplast is capable of expressing the selectable marker, thereby allowing for the measurement of modulated selectable marker expression as an alternative indicator for HR activation.

The invention relates to the field of genetic engineering and molecular biology, in particular to application of an SlGT2 gene in regulation and control of plant dwarfing. The nucleotide sequence of the SlGT2 gene is a nucleotide sequence as shown in SEQ ID NO: 1. The expression level of the SlGT2 gene is regulated and controlled through an SlGT2 overexpression plant so as to research the regulation and control mechanism of the SlGT2 gene on the tomato plant height. Results show that stem cells of the SlGT2 overexpressed tomato plant become smaller, the stem node spacing is shortened, and the plant height is reduced. Gene resources are provided for cultivating new tomato dwarfing varieties, potential application value is achieved, and meanwhile a theoretical basis is laid for researching a tomato dwarfing molecular mechanism.

The invention belongs to the field of molecular biology, and discloses an SNP (Single Nucleotide Polymorphism) molecular marker related to watermelon clausena lansium traits as well as a primer, a kit, application and a method for identifying the related traits, the primer of the SNP marker for identifying the watermelon clausena lansium traits comprises a forward primer F and a reverse primer R; the nucleotide sequences of the forward primer F and the reverse primer R are shown as'primer sequences'. The primer of the SNP marker for watermelon clausena lansium character identification can rapidly identify watermelon clausena lansium characters in the seedling stage, and can effectively solve the problems of low phenotypic identification result reliability, time consumption, high cost, high difficulty and the like.

The present invention relates to the fields of agriculture, plant biotechnology, and molecular biology. Specifically, the present invention relates to a wheat EPSPS mutant protein and use thereof in improving the tolerance of wheat to glyphosate. The mutant protein comprises an amino acid sequence having the following mutations compared to the EPSPS amino acid sequence of any genome of wild-type wheat: in the amino acid sequence set forth in SEQ ID NO: 2, the amino acid at position 168 is mutated from threonine to isoleucine, the amino acid at position 169 is mutated from alanine to valine, and the amino acid at position 172 is mutated from proline to serine. The mutant protein enables the production of stable glyphosate-resistant wheat, showing important significance for wheat breeding.

The invention belongs to the field of microbiology and molecular biology, and particularly relates to a method for extracting myxococcus flavus dormant mucospore RNA (Ribonucleic Acid). The method specifically comprises the following steps: taking a certain amount of myxococcus flavus liquid, and inducing sporocarp development by using a common starvation induction method; scraping the formed sporocarp structure, resuspending with sterile water, and destroying the sporocarp structure and mucospore coating by using a certain proportion of organic reagent; centrifuging, taking a water phase, adding an acetate buffer solution with a certain concentration, and precipitating with isopropanol to obtain nucleic acid substances; and removing DNA by using a conventional kit to obtain RNA. Aiming at the characteristic that the mucospore RNA is difficult to extract, the invention develops a simple, convenient and efficient method for extracting the mucospore RNA, and provides an effective way for deeply researching the ecological characteristics of the myxobacteria.

The invention belongs to the field of molecular biology, and particularly relates to an ITS molecular marker primer and method for identifying a melia azedarach lingering variety and application of the ITS molecular marker primer and method. The ITS molecular marker primer comprises the following components: ITS-p5, CCTTATCAYTTAGAGGAAGGAG, and ITS-u4, RGTTTCTTTCCTCCGCTTA, and is characterized in that the ITS-p5, the CCTTATCAYTTAGAGGAAGGAG The identification method comprises the following steps: collecting a molecular specimen; extracting DNA (deoxyribonucleic acid); according to the present invention, primers ITS-p5 and ITS-u4 are adopted to perform ITS fragment amplification, sequencing is performed, and if the 56th site to the 71st site in the 5 '-3' direction in the ITS sequences of different species of the chinaberry tree, the variety with the 16-base deletion of CGCGGGGCGGGCGA is the honeydew lingering variety; the invention also discloses an application of the ITS molecular marker primer in a reagent and a kit. According to the method, the ITS sequences of different species can be directly and accurately identified, the defects of traditional morphological identification are effectively overcome, the value of a new Honeysuckle variety in the fields of ecological restoration, economic material development, medicinal resource development and the like is fully exerted, and large-scale high-quality development of the melia azedarach industry is assisted.

The invention discloses a gingko GbDRF2 gene as well as an expression protein and application thereof, and belongs to the field of plant molecular biology. The GbDFR2 gene is obtained by analyzing and screening based on data of a ginkgo transcriptome in the earlier stage of a research group, the nucleotide sequence of the GbDFR2 gene is shown as SEQ ID NO.1, and the amino acid sequence of expression protein of the GbDFR2 gene is shown as SEQ ID NO.2. A GbDFR2 overexpression vector is constructed and nicotiana benthamiana is transformed, so that the synthesis of flavonoid substances in a transgenic plant is obviously changed, the contents of 9 flavonoid substances such as sakuranetin and 3, 7-dimethyl quercetin are increased, and the contents of 10 metabolites such as naringin and chalcone are decreased. The invention discloses a regulation effect of the ginkgo GbDFR2 gene in synthesis of flavonoid compounds, and provides a theoretical basis and a molecular tool for improving plant secondary metabolites through genetic engineering.

The invention belongs to the field of molecular biology, and particularly relates to application of an OsNiR1 gene to improvement of rice broad-spectrum disease resistance and improvement of rice agronomic traits. The invention provides an application of an OsNiR1 gene, and the application comprises an application in preventing and treating plant diseases and / or enhancing the resistance of plants to pathogenic bacteria, and / or an application in improving agronomic traits of the plants. The amino acid sequence coded by the OsNiR1 gene is as shown in SEQ ID NO. 1, and the CDS sequence of the OsNiR1 gene is as shown in SEQ ID NO. 2. The application proves that OsNiR1 participates in regulating and controlling the immune response pathway of rice to fungal disease rice blast, fungal disease banded sclerotial blight and bacterial disease bacterial leaf blight. Meanwhile, after the strong promoter ubi is adopted for driving, an overexpression material of the OsNiR1 is higher and stronger than a wild plant type, and the tiller number is increased. The invention provides important gene resources and molecular theoretical basis for developing a new variety of broad-spectrum disease-resistant rice.

The application belongs to the field of molecular biology, and particularly relates to a specific marker of an exon region of a Oplegnathus punctatus hmcn2 gene, a primer pair, a detection method, a kit and application. The specific marker of the exon region of the Oplegnathus punctatus hmcn2 gene is a nucleotide sequence of non-insertion and insertion specific markers of the exon region of the Oplegnathus punctatus hmcn2 gene DNA fragments, and the sequence is shown in bases of SEQ ID NO:1 and SEQ ID NO:2. The specific marker is applied to identification of female and male genetic sex of the Oplegnathus punctatus. The application establishes a method for realizing rapid genetic sex identification by rapidly detecting an exon DNA insertion variation of the Oplegnathus punctatus hmcn2 gene, and the method has a wide application prospect in the fields of sex identification of the Oplegnathus punctatus, preparation of high male seedlings and family selection breeding.

The invention relates to the field of gene engineering and molecular biology, in particular to application of a corn ZmMAPK6 gene in corn nitrogen utilization, and by constructing a CRISPR / Cas9 material, performing directional and fixed-point editing on a target gene and quickly knocking out related genes in a wild type, it is detected that the low nitrogen resistance of a transgenic plant is improved compared with that of the wild type. According to the application of the ZmMAPK6 gene in corn nitrogen utilization, a new germplasm resource is provided for cultivating a new nitrogen-efficient corn variety, and meanwhile, a certain theoretical basis is laid for researching plant low-nitrogen response and improving the plant nitrogen utilization efficiency.

The application provides a plasmid containing modified satellite RNA and a construction method and application, and belongs to the field of plant virology and molecular biology. Each copy number of the modified satellite RNA is composed of a modified satRNA TA-Tb fragment and an HDV ribozyme fragment, and the copy number of the modified satellite RNA in the plasmid is 10. The nucleotide sequence of the modified satellite RNA is shown in SEQ No. 3. After the plasmid of the application is inoculated by agrobacterium injection, the symptoms caused by cucumber mosaic virus on different strains and the same strain (CMV Fny ) on different hosts can be significantly reduced. An effective control measure for field control of cucumber mosaic virus is provided.

The present invention belongs to the field of plant molecular biology and relates to the excision of recombinant DNA from the genome of plant somatic cells.

The invention relates to the field of plant molecular biology, in particular to an SNP (Single Nucleotide Polymorphism) molecular marker combination for identifying length and width of upland cotton seeds and application of the SNP molecular marker combination. The SNP molecular marker combination provided by the invention comprises 12 molecular markers, and the 12 SNP molecular markers are all located on an A07 chromosome of a reference genome Gossypium hirsutum (AD1) 'TM1' gene CRIv1 of upland cotton. The SNP molecular marker combination provided by the invention exists in the form of DNA, can be detected in each tissue and development stage of cotton, is not limited by environment and seasons, is suitable for rapid large-scale screening, can realize early prediction of cottonseed utilization value, provides key technical support for cottonseed genetic research, molecular marker-assisted breeding and whole genome selection, and has a wide application prospect. The cotton molecular breeding efficiency and accuracy are improved.

The present invention discloses a SNP molecular marker associated with the testicle size trait of Hu sheep, its screening method, and application, belonging to the fields of genetic breeding and molecular biology. The marker is located in the intron region of the LRRIQ1 gene on chromosome 3 of Hu sheep and is significantly associated with the testicle size trait of Hu sheep. Through a scientific and rigorous screening method, the accuracy and reliability of the SNP molecular marker are ensured; the marker can quickly and accurately screen out Hu sheep individuals associated with the testicle size trait, improve breeding efficiency and accuracy, accelerate the breeding process, reduce breeding costs, and enhance economic benefits; it helps to protect genetic diversity and improve the accuracy of semen quality evaluation, providing a powerful tool for Hu sheep breeding work and important basic data for studying the genetic mechanism of testicular development and function; it can also be used as an auxiliary diagnostic tool to assist in identifying potential genetic defects or disease risks, promoting the progress and development of breeding technology.

The invention discloses a molecular marker related to disease resistance of ricefield eel and application of the molecular marker, and relates to the biological field of aquaculture and molecular marker screening. According to the invention, SNP loci in SFRP2 and TC1A gene coding regions in a ricefield eel group are screened through sequence alignment, and the two genes and a combination thereof are subjected to genetic typing; and comparing the differences of the disease resistance of the genotypes, and screening to obtain the genotype with the strongest disease resistance and the combination thereof, thereby providing an effective molecular marker for detecting the disease resistance of the ricefield eel. According to the monopterus albus disease resistance screening method developed based on the molecular marker, monopterus albus varieties with excellent disease resistance can be screened by utilizing SNP differences of disease-resistant genes, and then the monopterus albus varieties are used for disease resistance breeding. Effective technical support is provided for breeding of disease-resistant ricefield eel varieties.

The invention discloses an SNP (Single Nucleotide Polymorphism) molecular marker related to Hu sheep epididymis weight major quantitative trait sites and application thereof, and belongs to the fields of genetic breeding and molecular biology. The SNP molecular marker is located at the 33127527th basic group of Hu sheep chromosome 9, and polymorphism of two alleles C and T appears at the site. Different genotypes of the SNP site are significantly related to the epididymis weight of Hu sheep, wherein the epididymis weight of a CC genotype individual is significantly lower than that of TC and TT genotypes (Plt; 0.01) of the substrate. The SNP molecular marker is applied to early breeding of high-fecundity stud rams, and has important significance in improving the production capacity of sheep flock and increasing the breeding benefits.

The invention discloses a molecular marker related to broiler carcass traits and meat quality traits, an amplification primer and application of the molecular marker and the amplification primer, and belongs to the field of molecular biology. The nucleotide sequence of the molecular marker is as shown in SEQ ID NO.3; sNP1 with A / C mutation exists at the basic group at the 334th site of the sequence as shown in SEQ ID NO.3; sNP2 with A / G mutation exists at the basic group at the 362nd site; sNP3 with C / T mutation exists at the 518th basic group; an SNP4 with C / A mutation exists at the base at the 652nd site, an SNP5 with A / G mutation exists at the base at the 840th site, and an SNP6 with C / T mutation exists at the base at the 863rd site. By analyzing the LCA5L gene, it is found that the gene has a plurality of SNP loci significantly related to chicken carcass and meat quality traits, a new SNP molecular marker is provided for MAS, and meanwhile, a scientific basis is provided for chicken breeding.

The invention relates to the field of molecular biology, and discloses a haplotype marker for dairy cow milk production butter-fat percentage character breeding and application of the haplotype marker, the haplotype marker is composed of three SNP sites in a strong linkage imbalance state, and the three SNP sites are located in a Block1 haplotype block region of a 10 # chromosome NC037337.1 of a dairy cow ARS-UCD1.2 genome; the three SNP sites are as follows: SNP1: g.59680573 A > C, SNP2: g.59743173 G > C, and SNP3: g.59743414 C > T; the dominant haplotype of the haplotype marker is H2, and the allele combination of the haplotype marker is SNP1-A, SNP2-G and SNP3-C; the homozygous H2H2 of the dominant haplotype H2 is significantly related to the high milk fat percentage character of milk production of dairy cows. The bottleneck of the traditional technology is solved, and the breeding accuracy is remarkably improved; the screening detection technical scheme is stable and reliable and has a wide application range; the milk production quality of groups is improved, and the industrial economic value is enhanced.