384 results about "Biology (field)" patented technology

The invention discloses an SNP (Single Nucleotide Polymorphism) molecular marker related to the testis size character of Hu sheep as well as a screening method and application of the SNP molecular marker, and belongs to the fields of genetic breeding and molecular biology. The marker is located in an intron region of a Hu sheep chromosome 3 LRRIQ1 gene and is remarkably related to the Hu sheep testis size character, and the accuracy and reliability of the SNP molecular marker are ensured through a scientific and rigorous screening method; according to the marker, Hu sheep individuals related to testis size characters can be rapidly and accurately screened out, the breeding efficiency and accuracy are improved, the breeding process is accelerated, the breeding cost is reduced, and the economic benefits are improved; the genetic diversity is protected, the accuracy of seminal fluid quality evaluation is improved, a powerful tool is provided for Hu sheep breeding work, and important basic data is also provided for researching a genetic mechanism of testis development and functions; the method can also be used as an auxiliary diagnosis tool to assist in identifying potential genetic defects or disease risks and promote progress and development of the breeding technology.

The invention relates to a composition for identifying epinephelus fuscoguttatus, epinephelus coioides and hybrid offspring and application, and belongs to the field of molecular biology, the composition comprises a pair of primers and a group of probes, and the sequences are shown as SEQ ID NO.1-4. The invention further provides a kit for identifying the epinephelus fuscoguttatus, the epinephelus coioides and the hybrid offspring thereof, and the kit is used for identifying the epinephelus fuscoguttatus and the hybrid offspring of the epinephelus coioides and the hybrid offspring of the epinephelus coioides and the hybrid offspring of the epinephelus coioides. The kit comprises the composition disclosed by the invention. The composition can be used for rapidly identifying epinephelus fuscoguttatus, epinephelus coioides and hybrid filial generation gold and tiger hybrid spots of the epinephelus coioides, the epinephelus coioides and the hybrid filial generation gold and tiger hybrid spots of the epinephelus coioides and the epinephelus coioides and the hybrid filial generation gold and tiger hybrid spots of the epinephelus

The invention discloses a CDK5RAP3 gene SNP molecular marker related to the feed conversion rate of yellow feather broilers and application of the CDK5RAP3 gene SNP molecular marker, and belongs to the field of animal genetic breeding and molecular biology. The polymorphism of the SNP molecular marker is A / G, the SNP molecular marker is located at the 369307 site of a chicken chromosome 27, and the RS number of the SNP molecular marker is rs7383324012. The invention provides the SNP molecular marker related to the feed conversion rate of the yellow-feathered broiler chicken and located in the CDK5RAP3 gene of the 27th chromosome of the chicken, and the marker shows that the feed conversion rates of the yellow-feathered broiler chickens with different genotypes are obviously different, so that the SNP molecular marker can be used for efficiently screening individual or parent yellow-feathered broiler chickens with excellent characters of low feed conversion rate; the yellow-feather broiler chicken with low feed conversion rate can be quickly bred by applying the yellow-feather broiler chicken feed additive to breeding of the yellow-feather broiler chicken, the feed consumption in the production process can be effectively reduced, and the economic benefits and competitiveness of enterprises are improved.

The invention belongs to the field of molecular biology, and particularly relates to a transgenic soybean event LP207-1 and a detection method thereof. The invention provides a nucleic acid sequence for detecting a transgenic soybean event LP207-1, the nucleic acid sequence is selected from one or more of sequences SEQ ID NO: 1-7 or complementary sequences thereof, and the nucleic acid sequence is derived from a plant, a seed or a cell of the transgenic soybean event LP207-1. A representative sample of a seed of the transgenic soybean event LP207-1 is preserved in the China Center for Type Culture Collection on May 23, 2025, and the preservation number is CCTCC NO: P202516. The transgenic soybean event LP207-1 has good resistance to lepidoptera pests and can tolerate agricultural herbicides containing glyphosate and glufosinate-ammonium, economic loss caused by the lepidoptera pests can be avoided, and the yield is not reduced; and the breeding efficiency is enhanced, and the molecular marker can be used for tracking a breeding population and transgenic insertion fragments in offspring of the breeding population. The detection method provided by the invention can quickly, accurately and stably identify the existence of the plant material derived from the transgenic soybean event LP207-1.

The invention discloses a Chinese orchid flowering regulation gene CsFTL3 as well as an encoding protein and application thereof, and belongs to the field of plant molecular biology. The gene CsFTL3 for regulating and controlling flowering of Chinese cymbidium is separated from flower bud cDNA (complementary deoxyribonucleic acid) of a Chinese cymbidium variety 'Xiaoxiang', and 174 amino acids are encoded by the gene CsFTL3. Through construction of an expression vector of the CsFTL3 gene, overexpression in wild-type arabidopsis thaliana and heterologous overexpression of the CsFTL3 gene in the arabidopsis thaliana, it is found that bolting and flowering periods of the arabidopsis thaliana are significantly delayed, flower stems are significantly thickened and increased, the number of rosette leaves is significantly increased, and the growth period is prolonged, which indicates that the gene has the function of delaying the flowering time of the arabidopsis thaliana. The Chinese orchid flowering regulation and control gene can be used for molecular mechanism research of orchid flowering time, delay Chinese orchid flowering time, promote plant vegetative growth, prolong whole Chinese orchid viewing time and promote Chinese orchid genetic improvement and variety breeding.

The invention belongs to the field of pepper breeding and molecular biology, and discloses a KASP molecular marker closely linked with a pepper fruit color gene CaCHL1, the gene of pepper zunla-1 is used as a reference gene, and the single nucleotide polymorphism at the 13072545th basic group on a pepper chromosome 5 is subjected to basic group G-C replacement. The invention further discloses a primer and a kit for identifying the KASP molecular marker closely linked with the pepper fruit color gene CaCHL1 and application of the primer and the kit in identifying pepper fruit color types or molecular assisted breeding. The screened KASP molecular marker can be directly used for identifying the pepper fruit color, then auxiliary breeding is carried out by depending on the molecular marker, the problems that conventional breeding is long in period and prone to being influenced by the environment can be effectively solved, and the KASP molecular marker has important significance in pepper fruit color breeding practice and research on fruit color change and regulation and control mechanisms.

The invention discloses an SNP molecular marker related to drought resistance of corn and application of the SNP molecular marker, and relates to the field of agricultural molecular biology. The SNP molecular marker comprises at least one of a first SNP molecular marker and a second SNP molecular marker; the first SNP molecular marker is located at a basic group at a 97605381 site of a chromosome 9 of a corn reference genome Zeamas.AGPv4, and the polymorphism of the first SNP molecular marker is C / T; the second SNP molecular marker is located at the basic group of the 97604011 site of the No.9 chromosome of the corn reference genome Zeamas.AGPv4, and the polymorphism is C / T. The SNP molecular marker disclosed by the invention has the characteristics of high specificity, high sensitivity, high resolution and high typing quality; the drought resistance of the corn material can be quickly judged according to the genotype, and the method has wide application universality and can be used for corn breeding.

The invention belongs to the field of pepper breeding and molecular biology, and discloses an SNP (Single Nucleotide Polymorphism) molecular marker closely linked with pepper fruit length, which takes a camphor harbor as a reference, and a base C-to-A replacement occurs at a single nucleotide polymorphism at a 4397410 site base of a pepper No.3 chromosome. The invention further discloses an obtaining method of the SNP molecular marker closely linked with the length of the pepper fruit, a primer and a kit for identifying the SNP molecular marker closely linked with the length of the pepper fruit, and application of the primer and the kit in identification of different length types of the pepper fruit or molecular assisted breeding. The SNP molecular marker closely linked with the length of the pepper fruit is screened, the primer and the kit are developed for the molecular marker, the SNP molecular marker can be used for identifying the shape of the pepper fruit, then auxiliary breeding is carried out by depending on the molecular marker, the identification period is shortened, and the selection efficiency and accuracy are greatly improved.

The invention relates to the field of molecular biology, and discloses a fish DNA bar code database and a construction method and application thereof based on a mitochondrial genome complete sequence. The method comprises the steps of fish directory determination, fish collection, classification and identification, DNA extraction, mitochondrial whole genome sequencing and analysis, DNA bar code database building and database application. Based on the research basis of the target water area and fish historical data, the fish directory in the target water area is determined, the fish DNA bar code database is constructed by utilizing the mitochondrial whole genome sequencing technology, and accurate database support is provided for fish investigation and monitoring by applying the environment DNA technology. The problems that an existing fish public database is insufficient in integrity, miscellaneous in data source, uneven in quality and insufficient in region pertinence, possibly causes small population species comparison loss, comparison results are wrong, false positive results such as non-target region species cannot be effectively eliminated, and related species with high gene similarity are difficult to accurately identify are solved.

The invention relates to a river ecosystem planktonic bacteria environment threshold identification method, and belongs to the field of environmental ecology and computational biology. The method comprises the following steps: collecting a plurality of environmental factors, fusing and constructing a space-time aligned watershed ecosystem data set, and preprocessing the data set; constructing a prediction model and performing training; environmental factors are collected from a target drainage basin and input into the trained prediction model, and a nonlinear mapping relation between the environmental factors and the microbial community, namely an SHAP value, is obtained through learning; constructing a GAM response curve based on the SHAP values and the environmental factors, fitting a nonlinear response trend of the GAM response curve through a spline function, and identifying an environmental threshold corresponding to each environmental factor; through a Bootstrap resampling method, multiple times of environment threshold recognition are carried out on each environment factor, and a confidence interval is estimated based on environment thresholds obtained through multiple times of recognition, so that the stability and reliability of environment threshold recognition are improved.

The invention belongs to the field of molecular biology, and discloses acquisition of a waterlogging-resistant major QTL qWT1.1 in a corn seedling stage and development and application of a molecular marker primer of the waterlogging-resistant major QTL qWT1.1. The invention provides a major QTL qWT1.1 for controlling the waterlogging survival rate in the corn seedling stage, the major QTL qWT1.1 is located at the fifth version physical position of a reference genome in the first staining of the corn, the fifth version physical position of the reference genome is Chr1: 25904794-27039204, the major QTL site is used for controlling waterlogging-resistant phenotypic variation, an excellent haplotype SNP marker closely linked with the major QTL site is located at the 27039148th basic group of a first chromosome of a reference genome of the corn B73, and the excellent haplotype SNP marker is located at the 27039148th basic group of a second chromosome of the reference genome of the corn B73. The explained waterlogging survival rate phenotypic contribution rate is 10.03%, the PARMS marker designed by the SNP is used for detecting a corn inbred line group, the operation is simple and convenient, the typing is clear, the good selection effect on characters such as root length, root number, plant height, branch number and yield under the stress of corn waterlogging is realized, and the application prospect is wide. The molecular marker can be used for molecular marker-assisted selective breeding of the waterlogging resistance character in the corn seedling stage.

The invention provides a miRNA reference gene in the opening and senescence process of sweet osmanthus petals and a primer and application of the miRNA reference gene, and belongs to the field of plant molecular biology. The petal opening and senescence process comprises S1 (the pedicel period), S2 (the initial flowering period), S3 (the initial stage of full-bloom), S4 (the full-bloom period), S5 (the later stage of full-bloom) and S6 (the petal falling period). According to the invention, 14 candidate reference genes are screened, and the stability of the candidate genes is evaluated through five algorithms (delta-CT, geNorm, NormFinder, BestKeeper and RefFinder), so that miRNA internal references, namely novel33 and ofr-miR395e, which are suitable for the opening and senescence processes of the sweet-scented osmanthus petals are obtained. According to the invention, the real-time fluorescent quantitative PCR primer of the internal reference is designed, the primer has strong specificity and high amplification efficiency, the detection efficiency of real-time fluorescent quantitative detection of sweet osmanthus miRNA can be greatly improved, and the credibility of the detection result is improved.

The invention belongs to the field of molecular biology, and particularly relates to application of OsDOF3 protein or a coding gene thereof to improvement of rice broad-spectrum disease resistance and regulation of agronomic traits. According to the application, an OsDOF3 knockout mutant and an overexpression transgenic line are constructed under the background of susceptible rice, resistance phenotype analysis is performed by inoculating various pathogenic bacteria (including pyricularia oryzae, rhizoctonia solani and bacterial blight), and it is proved that OsDOF3 participates in regulation and control of an immunoreaction pathway of rice to fungal diseases (rice blast and sheath blight) and bacterial diseases (bacterial blight). Meanwhile, the application also finds that after a strong promoter ubi is adopted for driving, an overexpression material of the OsDOF3 is shorter than that of a wild plant, and the OsDOF3 influences normal growth and development of rice while stimulating plant immune response. The invention provides important gene resources and molecular theoretical basis for developing a new variety of broad-spectrum disease-resistant rice.

The invention discloses an SNP molecular marker related to dry and wet environment adaptability of sheep and application of the SNP molecular marker, and belongs to the field of molecular biology. The SNP molecular marker related to the humid environment adaptability of the sheep is located in an intron region of a BMPR1B gene, the gene mutation of the site significantly affects the sheep, and when the genotype of the site is AA, the sheep is the sheep with dry environment adaptability; when the genotype of the locus is GG, the locus is a sheep adaptive to a humid environment, so that a new molecular breeding marker is provided for screening or introducing the sheep adaptive to the humid environment.

The invention provides a plant phenotype multi-precision information joint prediction method, and relates to the technical field of plant phenotype prediction, and the method comprises the steps: data acquisition and preprocessing; constructing and training a model; through interpretability analysis, the method can be applied to different species and various plant traits and can be expanded to other biological fields; new omics data and analysis functions can be flexibly supplemented, and the future requirements of large-scale multi-omics research are met; compared with a traditional linear or general machine learning method, the deep learning model is better in performance in a complex gene interaction scene, and stable prediction performance is kept.

The invention belongs to the field of plant molecular biology, and particularly relates to an EjTGA1 gene related to loquat flowering phase and growth development regulation, a protein coded by the EjTGA1 gene and application of the EjTGA1 gene. The full length of the cDNA sequence of the gene is as shown in SEQ ID NO.1, and the amino acid sequence of the encoded protein of the gene is as shown in SEQ ID NO.2. The EjTGA1 gene disclosed by the invention is predominantly expressed in the loquat flower. An expression vector pCambia2300-EjTGA1-eGFP is introduced into wild type arabidopsis thaliana through an agrobacterium tumefaciens-mediated inflorescence infection method, the overexpression of transgenic arabidopsis thaliana 35S:: EjTGA1 promotes the arabidopsis thaliana to bloom in advance, and the leaf area and the rosette leaf number are increased. The transgenic arabidopsis thaliana plant material obtained by using the loquat EjTGA1 gene can advance flowering and fruiting time and regulate growth and development, and has a good application prospect.

The present invention relates to a mutant plasmid and a preparation method and application thereof, belonging to the field of plant virology and molecular biology. F R2‑2bPT‑PSTVd 1‑200 Contains CMV Fny The full-length RNA2 sequence of the isolate has TAATAG and PSTVd ​​fragment inserted between positions 2682 and 2683; the nucleotide sequence of the PSTVd ​​fragment is shown in SEQ ID NO.1. Fny Isolate RNA1 and modified PCB301‑RNA3‑ △ The apno plasmid and the mutant plasmid of the present invention are pre-mixed and inoculated. The attenuated vaccine can prevent the potato spindle tuber virus from causing harm and avoid the spread of the virus by aphids in the field. The effect is good and provides vaccine materials and effective measures for field control of PSTVd.

The invention discloses osmanthus transcription factors OfbHLH10 and OfWRKY78 for regulating synthesis of a flower fragrance component beta-ionone as well as a coding gene and application of the osmanthus transcription factors OfbHLH10 and OfWRKY78, and belongs to the field of plant molecular biology. The amino acid sequence of the osmanthus fragrans transcription factor OfbHLH10 provided by the invention is as shown in SEQ ID NO. 2, and the amino acid sequence of the transcription factor OfWRKY78 interacting with the osmanthus fragrans transcription factor OfbHLH10 is as shown in SEQ ID NO. 4. According to the invention, carriers constructed by CDs of OfWRKY78 and a promoter of OfCCD4 are jointly injected into Ben's tobacco leaves, a dual-luciferase experiment is carried out, and a result shows that OfbHLH10 has a promoting effect on expression of a synthetase gene OfCCD4 of beta-ionone, OfWRKY78 has an inhibiting effect on expression of OfCCD4, and OfbHLH10 and OfWRKY78 interact with each other and has an antagonistic effect on expression of OfCCD4. The gene provided by the invention can effectively regulate and control the content of beta-ionone in osmanthus fragrans fragrance, and has a good application prospect.

The invention relates to a penaeus monodon high temperature resistance character related SNP marker primer based on whole genome association analysis and application, and belongs to the field of molecular biology. The nucleotide sequence of the primer pair is shown in SEQ ID NO.1-2. An SNP molecular marker is located at the 27494780 site of the 27th chromosome of a penaeus monodon genome and is shown as Cgt; a mutation, and the A mutation is associated with a high temperature resistant character. The invention further provides a kit for detecting the high-temperature-resistant character of penaeus monodon, and high-temperature-resistant individuals can be screened by utilizing the primers and are used for breeding new varieties.

The invention discloses a multifunctional composite microbial inoculant for improving drought resistance of plants as well as a preparation method and application of the multifunctional composite microbial inoculant, and belongs to the field of microbiology. According to the multifunctional composite microbial inoculant, by establishing a culture medium with gradually increased PEG (Polyethylene Glycol) concentration, progressive drought resistance induction is carried out on a known functional strain, so that the strain damage caused by single high-intensity stress is effectively avoided, and the strain can still keep relatively high activity and stability under a drought condition; progressive drought-resistant induction enables the strain to gradually adapt to the drought environment, so that the drought resistance of the strain is enhanced, the survival rate of the strain under the drought condition is increased, and the strain can better play a role in drought resistance and growth promotion; the multifunctional composite microbial inoculant is applied to plant seedling root system field planting, stress resistance phenotype induction and ecological adaptability improvement in arid / semi-arid regions, the colonization survival rate of seedling rhizosphere of caragana microphylla is increased to 2.3 times of that of conventional treatment, the transplanting survival rate of caragana microphylla is increased to 78% from 42%, and the multifunctional composite microbial inoculant has wide application prospects.

The invention relates to an SNP molecular marker combination related to stichopus japonicus polysaccharide traits and application, belongs to the field of molecular biology, and provides an SNP molecular marker combination related to the stichopus japonicus polysaccharide content screened based on a whole genome association analysis technology, and a detection kit and a chip developed based on the SNP molecular markers. In a stichopus japonicus breeding procedure, individuals with more than one character dominant mutation site of the SNP molecular marker are screened as parents, so that the stichopus japonicus breeding process can be promoted; or offspring seeds with more than one character dominant mutation site of the SNP molecular marker are selected for breeding in the breeding process, so that the content of polysaccharide in the stichopus japonicus can be remarkably increased, and the economic value and the market competitiveness of the stichopus japonicus are improved.

The invention relates to a primer group of a microsatellite marker HA223 related to growth of belly-expanding hippocampus and application of the primer group, and belongs to the field of molecular biology, and the sequence of the primer group is shown as SEQ ID NO.1-2. The invention also provides an application of the primer group in breeding of growth traits of hippocampus erectus, the primer group is utilized to perform PCR amplification on DNA of a hippocampus erectus individual, an individual which can amplify a specific fragment with the size of 260bp is screened as a basic parent, and a heterozygous individual or a homozygous individual with the fragment is produced and used for breeding of a rapid growth variety, so that the breeding of the growth traits of the hippocampus erectus is facilitated, and the breeding efficiency of the hippocampus erectus is improved. Compared with a conventional breeding method, the method can shorten the breeding period, accelerate the breeding process and improve the breeding efficiency.

The invention relates to the field of plant molecular biology, in particular to a gene for controlling stem diameter and height of a tomato plant and application of the gene, the gene is an MYC1 gene, and the MYC1 gene is a nucleotide sequence capable of coding the following protein (a) or (b): (a) a protein composed of an amino acid sequence shown in SEQ ID NO: 2; and (b) a protein which is derived from (a) and has the same enzyme activity, wherein one or more amino acid sequences are substituted, deleted or added into the amino acid sequence in (a). The gene can control the stem diameter and height of a tomato plant, and by increasing the expression quantity of the MYC1 gene in the tomato plant, the stem of the tomato plant can be thickened, the internode spacing of the tomato plant can be shortened, and the tomato plant can be shortened. Furthermore, the stem diameters and heights of different plants and the subsequent cultivation of varieties with different stem diameters and heights can be controlled through a subsequent cloning technology.

The invention discloses a CAPS molecular marker of a GhFRO7 gene of upland cotton and application of the CAPS molecular marker in early blossoming character identification and breeding, and belongs to the field of molecular biology. Whole genome association analysis is carried out based on a high-density SNP marker, an SNP site which is located on an upland cotton GhFRO7 gene and is remarkably related to the flowering time character is identified and obtained, and then a CAPS molecular marker with the sequence shown as SEQ ID NO.34 and a primer pair with the sequence shown as SEQ ID NO.3-4 are developed. The CAPS molecular marker and the primer pair thereof provided by the invention can be applied to identification of early flowering upland cotton varieties and molecular marker-assisted selective breeding, and can effectively save the detection cost, shorten the period and improve the detection efficiency. The invention provides a new method for genetic improvement of cotton prematurity, and provides important genetic resources for developing cotton varieties adapting to specific climate conditions.

The invention discloses a KASP molecular marker related to gibberellic disease resistance on a wheat 7B chromosome and application of the KASP molecular marker, and belongs to the field of molecular biology. The KASP molecular marker closely associated with wheat scab resistance is screened, and the nucleotide sequence of the KASP molecular marker is as shown in SEQ ID NO.1; a primer group for amplifying the KASP molecular marker is further designed, and the primer group comprises upstream primers F1 and F2 and a downstream primer R which are connected with fluorescent connectors with different colors. Experimental results show that the KASP molecular marker developed by the invention can be used for efficiently and accurately identifying whether the wheat to be detected has gibberellic disease resistance or not so as to separate gibberellic disease resistant single plants in a population for improved breeding of the wheat, so that the detection cost can be effectively saved, the period can be shortened, and the breeding efficiency can be improved. The invention provides a new theoretical basis and technical means for disease-resistant breeding of wheat, and has outstanding industrial application value.

The invention provides a method for evaluating the hemorrhagic disease resistance of grass carp based on intestinal mucosal barrier multi-dimensional indexes, and relates to the field of aquaculture and molecular biology. According to the method, grass carps with different genetic backgrounds are taken as objects, and comparison shows that after GCRV infection for 48 hours, the intestinal mucosa permeability of gynogenetic grass carps and disease-resistant grass carps is remarkably lower than that of common grass carps, the intestinal mucosa villus length is remarkably higher than that of the common grass carps, and the expression of intestinal mucosa tight junction molecules is remarkably higher than that of the common grass carps; expression of intestinal mucosa immune related factors is obviously higher than that of common grass carp, diversity and abundance of intestinal flora are obviously higher than that of common grass carp, concentration of four key antioxidant enzymes in serum is obviously higher than that of common grass carp, and content of liver and intestinal VP56 is obviously lower than that of common grass carp. Therefore, after 15 days of GCRV infection, the survival rate of gynogenesis grass carps and disease-resistant grass carps is significantly higher than that of common grass carps. The invention provides a novel method for evaluating the hemorrhagic disease resistance of the grass carp.

The invention discloses a transcription factor OfMYB187 for regulating synthesis of beta-ionone of a sweet osmanthus fragrance component, an interaction protein OfCLpP of the transcription factor OfMYB187 as well as coding genes and application of the transcription factor OfMYB187 and the interaction protein OfCLpP, and belongs to the field of plant molecular biology. The amino acid sequence of the transcription factor OfMYB187 provided by the invention is as shown in SEQ ID NO. 2, and the amino acid sequence of the interaction protein OfCLpP of the transcription factor OfMYB187 is as shown in SEQ ID NO. 4. According to the application disclosed by the invention, a carrier constructed by promoters of OfMYB187, OfCLpP and OfCCD4 is jointly injected into a Ben's tobacco leaf, firefly and renilla determination is carried out, and a result shows that the OfMYB187 has an inhibition effect on a synthetase gene OfCCD4 of beta-ionone, but after interaction of the OfMYB187 and the OfCLpP, the OfMYB187 protein can be hydrolyzed, and the inhibition effect of the OfMYB187 protein on the OfCCD4 is relieved. The gene provided by the invention can directly or indirectly regulate and control the content of beta-ionone in osmanthus fragrans fragrance, and has a good application prospect.

The invention relates to a method for identifying Langya chicken and Non-Langya chicken by using SNP molecular markers, and belongs to the technical fields of animal husbandry and molecular biology. The SNP molecular marker for Langya chicken identification comprises 12 loci. The invention further provides a primer combination which comprises SNP loci for amplifying Langya chicken identification. According to the method, the 12 specific SNP sites of the Langya chicken are utilized, the site information is obtained through PCR amplification and Sanger sequencing, prediction is conducted through machine learning, the Langya chicken variety identification model is established based on the 12 SNP site combination, and the method has the advantages of being low in cost, simple in sequencing method, high in speed and high in accuracy.

The present invention relates generally to the field of molecular biology and concerns a method for high throughput screening and characterization of one or more candidate plant pathogen effect genes and / or one or more candidate plant disease resistance genes in a single plant cell, the method comprises: (i) introducing into a plant protoplast one or more expression cassettes comprising at least one candidate plant pathogen effect gene and / or at least one candidate plant disease resistance gene; (ii) transiently expressing the one or more candidate plant pathogen effect genes and / or the one or more candidate plant disease resistance genes; (iii) measuring reactive oxygen species (ROS) produced by the protoplast; then (iv) separating the plant protoplast producing the ROS; and (v) identifying the introduced at least one candidate plant pathogen effect gene and / or at least one candidate plant disease resistance gene. Alternatively, the protoplast is capable of expressing the selectable marker, thereby allowing for the measurement of modulated selectable marker expression as an alternative indicator for HR activation.

Embodiments disclosed herein relate to the field of plant molecular biology, specifically to DNA constructs for conferring insect resistance to a plant. Embodiments disclosed herein relate to insect resistant corn plant containing event DP-910521-2, and to assays for detecting the presence of event DP-910521-2 in samples and compositions thereof.