28 results about "Biology (field)" patented technology

The application provides a plasmid containing modified satellite RNA and a construction method and application, and belongs to the field of plant virology and molecular biology. Each copy number of the modified satellite RNA is composed of a modified satRNA TA-Tb fragment and an HDV ribozyme fragment, and the copy number of the modified satellite RNA in the plasmid is 10. The nucleotide sequence of the modified satellite RNA is shown in SEQ No. 3. After the plasmid of the application is inoculated by agrobacterium injection, the symptoms caused by cucumber mosaic virus on different strains and the same strain (CMV Fny ) on different hosts can be significantly reduced. An effective control measure for field control of cucumber mosaic virus is provided.

This invention discloses the application of the tomato SlPIF8b gene in regulating tomato fruit coloring and ripening, relating to the fields of genetic engineering and molecular biology. The tomato SlPIF8b gene has the nucleotide sequence shown in SEQ ID NO: 2. This application utilizes gene editing technology to knock out the SlPIF8b gene in tomatoes, which promotes the accumulation of lycopene and carotenoids in the tomato fruit, accelerates fruit coloring, and simultaneously promotes ethylene accumulation and softening, thus accelerating fruit ripening. This provides new germplasm resources for breeding new tomato varieties that promote fruit coloring and ripening, and has good potential application value. It also provides important theoretical basis and technical support for studying the molecular mechanisms of tomato ripening and improving the quality and nutritional value of tomato fruits.

The application discloses an InDel molecular marker related to peanut crown rot resistance and application, and belongs to the field of molecular biology.The polymorphism of the InDel molecular marker on an A01 chromosome is that a 177 bp nucleotide sequence exists or is lost, and the nucleotide sequence is shown as SEQ ID NO.5.The molecular marker is based on a resistance major gene qCRA01 The co-dominant marker developed from the candidate gene is closely related to the resistance trait, and rapid resistance identification and screening of peanut breeding materials and germplasm resources can be realized.The application provides a reliable tool for peanut crown rot resistance molecular marker assisted breeding. Early selection is carried out by using the molecular marker, the workload of field identification can be greatly reduced, the introduction and aggregation of disease resistance genes to excellent backgrounds can be accelerated, the breeding period is shortened, and the breeding efficiency is improved, and therefore the application has important application value for cultivating high-yield, high-quality and disease-resistant peanut new varieties.

This invention relates to the field of plant molecular biology, specifically to a loquat fatty acid desaturase-related gene, EjFAD8, its encoded protein, and its applications. The full-length cDNA sequence of this gene is shown in SEQ ID No. 1, and the amino acid sequence of its encoded protein is shown in SEQ ID No. 2. The EjFAD8 gene of this invention increases plant cold resistance by increasing the synthesis of SQDG (16:0 / 18:1) and promoting the expression of related genes in the ICE-CBF-COR low-temperature stress pathway. Using Agrobacterium tumefaciens Gv3101-mediated flower-dip method, the plant expression vector pCambia2300-35S::EjFAD8-eGFP containing the target gene was transformed into wild-type Arabidopsis thaliana. Overexpression of 35S::EjFAD8 in the transgenic Arabidopsis enhanced its cold resistance. This invention utilizes transgenic Arabidopsis thaliana plant materials obtained from the loquat EjFAD8 gene. Under low temperature stress, these plants can increase the content of unsaturated fatty acids, enhance the expression of low temperature-related genes, and reduce the content of H2O2 in the plant, thereby increasing the plant's cold resistance and showing great application potential.

The present application relates to the field of molecular biology, further to a specific marker of Oplegnathus fasciatus hydin gene exon region insertion variation, a primer pair and application and a detection method.The specific marker of Oplegnathus fasciatus hydin gene exon region insertion variation is a nucleotide sequence of a non-insertion and insertion specific marker of the Oplegnathus fasciatus hydin gene exon region DNA fragment, and the sequence is shown in the bases of SEQ ID NO:1 and SEQ ID NO:2.The detection method provided by the present application is simple in operation, clear in result, good in repeatability, significantly shortens the identification period, improves the detection throughput and accuracy, and has important application value in Oplegnathus fasciatus gender identification, high male seedling preparation and family selection breeding and other breeding practices.

This invention belongs to the field of plant molecular biology, specifically relating to a small RNA molecule that alleviates lignification of loquat fruit and its application. The small RNA molecule is from loquat. Eja-MIR164 Its nucleotide sequence is shown in SEQ ID NO. 6. After processing within the plant, it forms a functional mature form. This invention experimentally confirms that loquat fruit contains… Eja-MIR164 The expression level of Eja-miR164 was negatively correlated with lignin content; transient interference or stable transformation using STTM technology reduced Eja-miR164 content, targeting its genes NAC transcription factor and lignin polymerase gene. EjLAC7 Increased expression levels in all samples led to a significant increase in lignin content. This invention utilizes small RNA molecules. Eja- MIR164 It can be used to breed new loquat varieties with reduced lignification, improve fruit texture and quality, and enhance post-harvest storage performance, and has potential application value in loquat genetic improvement and molecular breeding.

The application discloses a kind of small RNA fusion multiomics plant phenotype prediction method, it is related to bioinformatics and agricultural biology field, including the following steps: obtaining and pre-processing genotype, transcript expression and small RNA expression data, obtain standardized feature matrix;The standardized feature matrix is screened based on the feature of pearson correlation, and the corresponding genotype, transcript expression, small RNA expression feature subset is obtained;Based on the pre-trained MirGP prediction model, by multi-branch feature extraction, multi-level efficient channel attention fusion and regression prediction, output plant phenotype prediction result.The method introduces multiple small RNA regulation characteristics and constructs hierarchical deep learning fusion architecture, expands the multiomics feature dimension, reduces data redundancy, realizes the effective integration and deep mining of cross-modal features.

The application belongs to the field of molecular biology, and discloses obtaining of a main-effect QTL qWTCKNR1.2 of corn yield tolerance to waterlogging, and development and application of a molecular marker primer thereof. The application provides that the main-effect QTL of the waterlogging tolerance coefficient of corn row kernel number is located at the 26373756th base of the first chromosome of a corn B73 reference genome, and the explained phenotype contribution rate of the waterlogging tolerance coefficient of the row kernel number is 5.94%. The PARMS marker designed by using the SNP is used for detecting a corn inbred line population, and it is found that the PARMS marker is simple in operation and clear in typing, and has a good selection effect on root fresh weight, root dry weight, root length, leaf number on ear, ear length, ear thickness, ear weight and other traits of corn under waterlogging stress, and can be used for molecular marker assisted selection breeding of the waterlogging tolerance traits of corn yield.

This invention belongs to the field of molecular biology, specifically involving SbJMJ02 The application of genes or their expression promoters in improving plant drought and / or salt tolerance. SbJMJ02 The nucleotide sequence of the gene is shown in SEQ ID NO.1, and the amino acid sequence of the protein it encodes is shown in SEQ ID NO.2. This invention has experimentally demonstrated that... SbJMJ02 The gene's expression was significantly upregulated under drought and salt stress conditions, and its encoded protein, when heterologously expressed in yeast and Arabidopsis, significantly enhanced the organisms' tolerance to high salt, high osmotic pressure, and drought stress. Based on this, the present invention provides... SbJMJ02 The application of genes, their encoded proteins, or expression promoters in improving plant drought and salt tolerance provides new genetic resources and molecular breeding strategies for the genetic improvement of crop stress resistance.

The application discloses a SNP marker-based genome DNA fingerprint of Herba Phrymatis, a gene chip and application thereof, and belongs to the field of molecular biology. The application obtains SNP of a genome of Herba Phrymatis based on GBS, identifies 248 core SNP sites, and develops a SNP liquid-phase gene chip through a targeted capture sequencing technology, so that the sites uniformly cover a chromosome set, are representative, have high polymorphism, high specificity, and strong universality, are suitable for being applied to identification of Herba Phrymatis varieties, provide scientific and technological support for Herba Phrymatis variety management and quality detection, and lay a technical foundation for Herba Phrymatis germplasm resource analysis.

The application discloses a gene GbTG1 for improving salt tolerance of ginkgo, an expression protein thereof and application, and belongs to the field of plant molecular biology. The applicant screens and clones the ginkgo GbTG1 gene based on previous ginkgo transcriptome data, a nucleotide sequence of the gene is shown as SEQ ID NO. 1, and an amino acid sequence of the expression protein of the gene is shown as SEQ ID NO. 2. The transformation of the GbTG1 into tobacco shows that, compared with tobacco strains into which EV is transformed, the leaves of transgenic tobacco strains have no obvious dryness phenomenon observed by naked eyes. Measurement shows that the relative conductivity in the leaves of the transgenic tobacco strains is significantly reduced, and the average reduction is 9.4% compared with the leaves of the tobacco into which EV is transformed. Meanwhile, the content of malondialdehyde in the leaves and the relative conductivity show the same trend, and the content of the transgenic strains is averagely reduced by 18%. It is shown that the GbTG1 is a salt stress resistance gene of ginkgo, and has important application value.

The present application relates to the field of molecular biology, further to an Oplegnathus punctatus intergenic region specific marker of MPP2 / ELF3 gene, primer pair and application and detection method. The specific marker of the intergenic region insertion variation of the Oplegnathus punctatus MPP2 / ELF3 gene is a DNA fragment specific to the non-insertion and insertion of the intergenic region of the Oplegnathus punctatus MPP2 / ELF3 gene, and the nucleotide sequence of the DNA fragment is shown as SEQ ID NO:1 and SEQ ID NO:2. The present application amplifies 469bp and 185bp bands in individuals with DNA insertion variation by using a pair of primers, and only amplifies 185bp bands in individuals without insertion variation. The detection method of the present application significantly shortens the identification time, improves the detection efficiency, and has important application value in the sex identification and family selection of the offspring of the hybridization between Oplegnathus punctatus males and Oplegnathus stripatus females.

A culture medium relating to the field of molecular biology and more particularly that of clinical and industrial microbiology, and for detecting microorganisms, including, as a gelling agent, a mixture of agar and kappa-carrageenan.

This invention belongs to the field of molecular biology and discloses the main QTL for maize yield tolerance to waterlogging. qWTCKNR3.2 This invention provides the acquisition of [specific compound name], the development and application of its molecular marker primers, and the main effect QTL for controlling the waterlogging tolerance coefficient of maize rows. qWTCKNR3.2 Located at nucleotide 218456862 on chromosome 3 of the maize B73 reference genome, this SNP contributed 14.53% to the phenotypic contribution of row number to waterlogging tolerance. PARMS markers designed using this SNP were used to test maize inbred lines, revealing that the method is simple to operate and produces clear genotypes. Furthermore, it demonstrated good selection effects on traits such as plant height, ear position, number of tassel branches, tassel main axis length, leaf length, number of ear rows, ear weight, and single ear grain weight under waterlogging stress. Therefore, it can be used for marker-assisted selection breeding of maize yield and waterlogging tolerance traits.

The application discloses a crab meat DNA extraction kit and an extraction method, and belongs to the field of molecular biology. The kit comprises a lysis solution, and the lysis solution comprises SDS, protease K and RNase A. The kit is simple in components, easy to prepare, low in cost and free of toxicity. The extraction method is simple and easy to operate, and crab meat DNA can be quickly extracted, thereby promoting the high-quality and sustainable development of the aquaculture industry.

This invention belongs to the field of molecular biology and provides a method for virus-induced gene silencing in cauliflower. The method includes: vacuum-infecting cauliflower seedlings with a transformation bacterial solution followed by a first dark culture; then, culturing the seedlings under light after the first dark culture; then, injecting the seedlings after the light culture with the transformation bacterial solution; and finally, culturing the injected seedlings under dark a second time. The transformation bacterial solution comprises: Agrobacterium-tuberculosis solution transformed with the PCVA-PDS recombinant silencing vector and Agrobacterium-tuberculosis solution transformed with the PCVB helper vector. This invention successfully achieves effective silencing of the PDS gene in cauliflower, with a silencing efficiency of over 60%, completely solving the technical problem of ineffective VIGS silencing in cauliflower. This method is convenient to operate, has a short cycle, strong adaptability, and broad application prospects.

The application discloses a genetic element for accelerating morphological differentiation of actinomycetes, and belongs to the fields of synthetic biology and molecular biology. eshA Gene overexpression vector pIB139-PermE-his- eshA -his is transformed into target actinomycetes streptomyces coelicolor, and an engineering strain is obtained through screening, and through plate phenotype observation and growth curve determination, the significant effect of gene overexpression is verified. eshA The overexpression strain O eshA S. coelicolor WT appears aerial hyphae and mature spores earlier. The application provides a simple and specific technical scheme for controlling the speed of morphological differentiation of actinomycetes, provides a new genetic element for accelerating morphological differentiation of actinomycetes, and has important application value.​

The present application relates to the field of molecular biology, and more particularly to a genetic sex-specific marker of Pelophylax nigromaculatus and application thereof, the nucleotide sequence of the marker is shown as SEQ ID NO:1, the specific marker provided by the present application can quickly and simply identify the genetic sex of Pelophylax nigromaculatus at each age stage, and is of great significance to the study of the sex determination and differentiation mechanism of Pelophylax nigromaculatus and the realization of the sex control thereof.

The application discloses a CDK5RAP3 gene SNP molecular marker related to yellow-feathered broiler feed conversion rate and application thereof, and belongs to the field of animal genetics and breeding and molecular biology. The SNP molecular marker polymorphism is A / G, is located at the 369307th site of chicken chromosome 27, and has an RS number of rs738324012. The application provides a SNP molecular marker related to yellow-feathered broiler feed conversion rate in a CDK5RAP3 gene on chicken chromosome 27, the marker indicates that there is a significant difference in feed conversion rate between yellow-feathered broilers with different genotypes, and the marker can be used for efficiently screening individuals or parent yellow-feathered broilers with excellent low feed conversion rate properties; when the marker is applied to yellow-feathered broiler breeding, yellow-feathered broilers with low feed conversion rate can be quickly bred, feed consumption in the production process can be effectively reduced, and economic benefits and competitiveness of enterprises can be improved.

Cucumber CsSTAR2 gene and its encoded protein are applied to reduce pesticide propamocarb residue, and the present application relates to the field of molecular biology.The nucleotide sequence of cucumber CsSTAR2 gene and the amino acid sequence coded by cucumber CsSTAR2 gene are disclosed.The protein expressed by CsSTAR2 gene, expression vector, transgenic cell or engineering bacteria are disclosed.The application of CsSTAR2 gene in reducing pesticide propamocarb residue is disclosed.In the expression mode of CsSTAR2 in response to propamocarb, it is shown that CsSTAR2 can quickly respond to the induction of propamocarb and play a function in low propamocarb residue of fruits.In the CsSTAR2 overexpression plant, the propamocarb residue of fruits is lower than that of wild type plant;the propamocarb residue in CsSTAR2 gene editing plant is higher than that of wild type plant, and there is a very significant difference, and CsSTAR2 has the function of positively regulating the low propamocarb residue of cucumber fruits.

This disclosure relates to the field of molecular biology. Provided are novel compositions and methods for mitigating undesirable agronomic characteristics of certain transgenic proteins, including insecticidal proteins, when expressed in transgenic plants. Also provided are novel compositions and methods for increasing protein expression levels in transgenic plants. Further provided are novel genes that encode pesticidal proteins. These pesticidal proteins and the nucleic acid sequences that encode them are useful in preparing pesticidal formulations and in the production of transgenic pest-resistant plants. Engineered peptides and chimeric fusion polypeptides, and methods of producing and using the same, are also contemplated.

This invention belongs to the field of molecular biology and discloses the acquisition of the major-effect QTL qWTCPH5.1 for maize yield and waterlogging tolerance, as well as the development and application of its molecular marker primers. The invention provides a major-effect QTL qWTCPH5.1 controlling the waterlogging tolerance coefficient of maize field yield, plant height, and waterlogging tolerance. Located at nucleotide 6892087 on chromosome 5 of the maize B73 reference genome, it explains 5.88% of the phenotypic contribution of waterlogging plant type, plant height, and waterlogging tolerance coefficient. PARMS markers designed using this SNP were used to detect maize inbred lines, revealing that the method is simple to operate, provides clear genotyping, and exhibits good selection effects on traits such as ear position, leaf length, leaf width, and yield under maize waterlogging stress. It can be used for marker-assisted selection breeding of maize field yield and waterlogging tolerance traits.

The present application relates to a kind of star point oriental tile genetic sex related InDel molecular marker, primer and its application, belong to molecular biology field, the molecular marker is located on the male DNA of star point oriental tile, the partial DNA sequence shown in the molecular marker is as shown in SEQ ID NO.1, the InDel molecular marker is a 2bp length insertion sequence, at the 35th and 36th of nucleotide sequence shown in SEQ ID NO.1, the sequence only stably exists in the genome of male individual of star point oriental tile, is deleted in the genome of female individual.This application can accurately and non-invasively identify the genetic sex of star point oriental tile fry and non-breeding season adult fish without killing experimental subjects.

Embodiments disclosed herein relate to the field of plant molecular biology, specifically to DNA constructs for conferring insect resistance to a plant. Embodiments disclosed herein relate to insect resistant soybean plant containing event COR-23134-4, and to assays for detecting the presence of event COR-23134-4 in samples and compositions thereof.

This invention belongs to the field of molecular biology and relates to animal genetics and breeding. It specifically provides genes, primers, applications, and methods for identifying the developmental stages of the clitellum in *Pheretima aspergillum*. Addressing the shortcomings of existing artificial breeding methods for *Pheretima aspergillum*, which rely heavily on visual observation of the clitellum and suffer from significant physiological lag, this invention is the first to discover and screen specific molecular marker genes closely related to the initiation and maturity stages of clitellum differentiation in *Pheretima aspergillum*, and provides highly specific amplification primer sets and detection methods. This invention achieves very early molecular early warning and objective quantitative identification of clitellum development in *Pheretima aspergillum*, providing molecular standards for the quantitative control of nutritional intervention and mating / harvesting timing in artificial breeding. This technology helps to break through the bottleneck of large-scale *Pheretima aspergillum* farming, reduce dependence on wild resources, and is of great significance for protecting ecological red lines and the safety of the traditional Chinese medicine industry.

This invention relates to the field of agricultural molecular biology and its application of markers for detecting soybean protein content. It addresses the problems of weak linkage and insufficient stability of some existing molecular markers with protein content traits. The invention utilizes dCAPS and KASP markers for detecting soybean protein content, and their application in high-protein crop breeding. The marker sites of this invention are stable and reliable, with high detection accuracy; identification is efficient and rapid, suitable for early screening; high throughput and strong adaptability reduce breeding costs; and significant application value contributes to targeted breeding. This invention achieves rapid, low-cost, and accurate detection of high-protein traits, providing an efficient technical tool for targeted breeding of high-protein crops.

This invention discloses primers for the rapid identification of E-genome cotton species in diploid and tetraploid Gossypium and their applications, belonging to the field of molecular biology. Using the primers provided by this invention for the rapid identification of E-genome cotton species in diploid and tetraploid Gossypium, a nucleotide fragment of approximately 350 bp can be specifically amplified in PCR reactions using E-genome cotton species DNA as a template; however, no amplification products are obtained in PCR reactions using genomic DNA from other diploid or tetraploid cotton species as templates, demonstrating high specificity. This method enables rapid and accurate identification of E-genome cotton species, effectively solving the problem of accidental confusion between E-genome cotton species DNA and other cotton species DNA in experimental work, filling the gap in rapid identification technology for E-genome cotton species, and has significant practical value.

The invention belongs to the field of molecular biology, and discloses a molecular marker for identifying seedless traits of Qingqing orange and Hubei orange No.1 and filial generation thereof and application of the molecular marker for identifying seedless traits of Qingqing orange and Hubei orange No.1 and filial generation thereof. The applicant constructs a hybrid population by hybridizing 'Qingqing orange' and 'Hubei orange No.1', and finally finds that A / G mutation exists in the 25753159th basic group of the chromosome 8 of the sweet orange through re-sequencing analysis of fertile offspring and sterile offspring BSA and male and female parents, and the mutation is related to the character of whether seeds are contained or not. Aiming at the site, the applicant designs a CAPS primer, so that the Qingqing orange, the Hubei orange No.1 and the filial generation of the Qingqing orange, the Hubei orange No.1 and the filial generation of the Qingqing orange and the Hubei orange No.1 can be subjected to grain identification, and compared with a traditional morphological identification or multi-marker combined identification method, the detection time is greatly shortened, and the detection accuracy is 100%.