954 results about "Biology (field)" patented technology

The present invention relates to the field of plant molecular biology, more particularly to the root-specific gene expression in plants. The invention provides nucleic acids for a novel transcriptional regulatory root-specific promoter and nucleic acid and protein sequences coding for a new LRR receptor-kinase protein, further specified as a root clavata 1 homolog (RCH1). Further provided are compositions comprising nucleic acids, polypeptides, antibodies and vectors. The invention further provides for methods for modifying cell fate and / or plant development and / or plant morphology and / or plant biochemistry and / or plant physiology comprising the modification of expression in particular cells, tissues or organs of a plant of the novel LRR receptor-like kinase or comprising the expressing of a gene of interest under the control of the novel transcriptional regulatory root-specific promoter. Further are provided compounds interacting with the new polypeptides for use as herbicides or growth regulators.

A biological chemosensorial reaction can be produced using a process and a chip system for the controlled emission of a substance or a mixture of substances that work chemosensorially. The chip system emits, in a controlled as well as programmable manner, the substance or a mixture of substances that trigger a biochemical transduction at a chemosensory receptor for a neuronally transmitted signal to a specific structure of the central nervous system. The new process and its devices render numerous new applications possible within the medical and biological areas for chemosensorially active substances.

The present invention relates to certain computational methods for classifying a plurality of objects or for identifying one or more latent classes among a plurality of objects. The present invention presents methods that glean relationships across at least two distinct sets of objects, allowing one to identify latent classes of objects along one set of margins, observations about which objects provide insight into possible properties or characteristics of objects along another set of margins. More specifically, the present invention relates to a process for analyzing multivariate sets of data utilizing tools that combine, for example, aspects of fuzzy logic and statistics. The present invention finds a number of practical applications, including the sensible analysis of large amounts of information, such as those generated sequence analysis, gene expression and proteomics in the field of biology.

The invention relates to the field of molecular biology and discloses a kit and a method for extracting DNA from histiocytes. The kit comprises a binding solution, a regulating and binding solution and a deproteinization solution, wherein the binding solution comprises 3-5M of guanidinium isothiocyanate, 5-50mM of Tris-HCl with a pH value of 3.5-5.5, 5-25 percent by weight of Ttiton-X 100 and 5-20mM of Urea; the regulating and binding solution is isopropyl alcohol; and the deproteinization solution comprises 2-6M of guanidine hydrochloride, 5-50mM of Tris-HCl with a pH value of 6.5-8.0 and 5-50 percent by weight of absolute ethyl alcohol. The kit also comprises a pretreatment buffer solution, a splitting solution, a magnetic bead, a rinsing solution and an eluting solution. The DNA extracted by the kit and the method has the advantages of high content and high extraction speed, and the invention does not adopt toxic reagents and is suitable for extracting the DNA of various histiocytes.

The invention relates to the field of molecular biology and discloses a kit and a method for extracting microbial DNA. The kit comprises lysis solution, inhibitor removal solution and binding solution, wherein the lysis solution comprises 50 to 200mM of Tris-HCl, 50 to 150mM of EDTA, 0.5 to 3M of NaCl, 0.5 to 2 percent of CTAB, 0.5 to 2 percent of PVP and 0.5 to 2 percent of SDS; the inhibitor removal solution comprises 100 to 300mM potassium acetate, sodium acetate or ammonium acetate and 50 to 200mM aluminum sulfate, ammonium sulfate or aluminum ammonium sulfate; and the binding solution comprises 3 to 6M guanidine hydrochloride, 10 to 50mM Tris-HCl and 5 to 50 percent isopropanol. The kit and the method for extracting the microbial DNA have the advantages of high purity, high universality, high extraction speed, direct use for a downstream experiment, and application to extracting DNA from a microbe-containing sample.

The invention relates to a method for establishing a ferret model capable of applying to study human disease and an application thereof, which belong to the biology field and specifically relates to a ferret ovulation induction technology and a ferret external fertilization technology. With the method for establishing the ferret model based on the technology of utilizing CRISPR / Cas9 on the above aspects, the method can be applied to study human diseases.

A machine reading system is described herein that includes a framework in which grammar rules can be developed using a concise language that combines syntax and semantics. The resulting technology thus reduces the development time for new grammars in a new domain. An enormous amount of information appears in the form of natural language across millions of academic papers and other literature sources. For example, in the biological domain, there is a tremendous ongoing effort to extract individual chemical interactions from these texts, but these interactions are only isolated fragments of larger causal mechanisms such as protein signaling pathways. The proposed rule-based event extraction framework can model underlying syntactic representations of events in order to extract signaling pathway fragments. Though application to the biomedical domain is herein described, the framework is domain-independent and is expressive enough to capture most complex events annotated by domain experts.

The invention provides a wheat TaAGO4a gene CRISPR / Cas9 (clustered regularly interspaced short palindromic repeats) / -CRISPR-associated protein 9) vector and application thereof and belongs to the field of crop molecular biology. The wheat TaAGO4a gene CRISPR / Cas9 vector and the application thereof have the advantages that gRNA of a third exon of specificity-targeted TaAGO4a is provided firstly, a DNA sequence of the gRNA is shown as SEQ ID NO.1, and the gRNA contains an enzyme cutting site XmnI; subsequently, the CRISPR / Cas9 vector containing the gRNA is provided, and through co-transformation of Cas9 and the specific gRNA into a wheat protoplast as well as enzyme cutting and sequencing technologies, the condition that the gRNA can guide the Cas9 to cut three copies positioned on a chromosome 3A, a chromosome 3B and a chromosome 3D of the TaAGO4a respectively can be detected successfully so as to cause frameshift mutation of the gene and result in afunction or excalation of the gene; the wheat TaAGO4a gene CRISPR / Cas9 vector can be used for preparing TaAGO4a gene-deleted transgenic wheat.

The invention belongs to the field of the tumor molecule biology, in particular to a diagnosis reagent, a reagent knit and prevention and cure drugs of endometrial carcinoma, which provides a new diagnosis reagent and medicine for the early diagnosis and the prevention of endometrial carcinoma, and also provides a new method for filtering the medicine used for preventing endometrial carcinoma. The diagnosis reagent for endometrial carcinoma of the invention contains a reagent which can test the expressing level of a human cyclophilin A protein or the content of the mRNA of genes of the human cyclophilin A protein. The medicine which can prevent endometrial carcinoma of the invention includes: the expressing carrier of a shPNA molecule which can express the human cyclophilin A, etc. can change the expressing level of the human cyclophilin A protein or the mRNA content of the genes of the human cyclophilin A protein. The diagnosis reagent of the invention can be used for the early diagnosis of endometrial carcinoma, which has a wide application prospect.

The invention relates to the molecular biology field and particularly relates to a gene knockout carrier, a construction method and application thereof and a gene knockout method of an NLRP1 gene of an MH7A cell. A CRISPR-Cas9 gene knockout system is utilized for carrying out CRISPR targeting sequence design by taking NLRP1 as a target gene so as to prepare a knockout carrier aiming at the NLRP1 gene, and the knockout carrier is utilized for transfecting the MH7A cell, so that the NLRP1 gene in the MH7A cell can be efficiently knocked out; and therefore, a research platform for rheumatoid arthritis is effectively built, so that the research of the pathogenesis of the rheumatoid arthritis is greatly promoted, and the researches of the interaction of NLRP1 inflammasomes and various inflammatory factors and the disease related molecular mechanisms of the rheumatoid arthritis and meningitis can be promoted.

The present invention relates generally to the field of molecular biology. More particularly, it concerns methods and compositions for detecting, evaluating, and / or mapping 5-hydroxymethyl-modified cytosine bases within a nucleic acid molecule.

The invention relates to the field of cytobiology and in particular relates to a method for preparing platelet lysate and application of the platelet lysate. The preparation method comprises the following steps: oscillating platelets at the temperature of 20-24 DEG C, preserving heat for 1-5 days, treating by adopting a repeated freeze-thaw method and an ultrasonic method, centrifuging, collecting the supernatant, and removing the fibrous proteins, thereby obtaining the platelet lysate. According to the method for preparing the platelet lysate provided by the invention, the content of cell factors in the platelet lysate can be obviously improved, quantitative balance of the cell factors can be realized, the differences among the product batches are reduced, and the effect of stably promoting human-derived cell growth can be achieved.

The invention relates to the field of molecular biology and discloses a method and a kit for extracting ribonucleic acid (RNA). The method for extracting the RNA comprises the following steps of: uniformly mixing a sample and lysis solution; centrifugally collecting supernate and uniformly mixing the supernate and absolute ethyl alcohol which is half of the volume of the supernate; combining the mixture with 0.45 mu m glass fiber cellulose acetate membrane; washing with deproteinization liquid and rinsing liquid; eluting adsorbed RNA; uniformly mixing lysis cells and an extraction aid; and centrifugally removing polysaccharide and polyphenol substances. The kit for extracting the RNA comprises the lysis solution, the deproteinization liquid, the rinsing liquid, the eluting liquid and the extraction aid. The method of the invention has the advantages of simpleness, rapidness, no use of toxic reagent, wide application range and capabilities of effectively removing the polysaccharide andpolyphenol substances and separating high-quality RNA out by adding the extraction aid. The kit of the invention has the advantages of no toxic reagent, wide application range, RNA extraction effectsof plant materials which are rich in polysaccharide and polyphenol superior to that of foreign kits, low cost and suitability for extensive laboratories and scientific researches.

The present invention relates generally to the field of molecular biology and concerns a method for improving plant growth characteristics relative to corresponding wild type plants. More specifically, the present invention concerns a method for improving plant growth characteristics comprising modulating expression in a plant of a nucleic acid encoding a class I homeodomain leucine zipper (HDZip) hox5 polypeptide or a homologue thereof; or comprising modulating expression in a plant of a nucleic acid encoding a nitrate transporter protein (NRT) or a homologue thereof; or comprising modulating expression in a plant of a nucleic acid encoding a polypeptide denoted Yield Enhancing Protein 16 (YEP16); or comprising modulating expression in a plant of a Group I glycogen synthase kinase (Group I shaggy-like kinase) or a homologue thereof. The present invention also concerns plants having modulated expression of a nucleic acid encoding a class I HDZip hox5 polypeptide or a homologue thereof; or having modulated expression of a nucleic acid encoding a NRT protein or a homologue thereof; or having modulated expression of a nucleic acid encoding a polypeptide denoted YEP16; or having modulated expression of a Group I shaggy-like kinase or a homologue thereof, which plants have improved growth characteristics relative to corresponding wild type plants. The invention also provides constructs useful in the methods of the invention.

The invention relates to the field of molecular biology and especially relates to a method and a kit for detecting gene point mutation based on a digital PCR platform. Digital PCR is used as a platform, PCR primers and TaqMan probes are added into a reaction system, the TaqMan probes labeled with different types of fluorescent light groups are used for detecting wild and mutational DNA templates, and according to fluorescent light types, types and an amount ratio of the DNA templates in a sample are determined.

The invention discloses a method for primary culture of tumor cells and relates to the field of cell biology. According to the method disclosed by the invention, a tumor sample is prepared into a single-cell suspension liquid by use of an enzyme method or a mechanical method, and then tumor cell bladders are prepared by use of a serum-free suspension culture method, wherein alkaline fibroblast growth factors, epidermal growth factors, insulin and bovine serum albumin need to be added during a culturing process, then the growth factors are removed, and the culture is implemented in a general culture medium to obtain adherent cells capable of realizing continuous passage. The method disclosed by the invention is short in culture period and has the effect of increasing the success rate of the primary culture of tumors.

The invention relates to the field of molecular biology and discloses a kit and a method for extracting DNA from micro samples. The kit comprises a binding liquid, a regulated binding liquid and a deproteinized liquid, wherein the binding liquid comprises 3-8M of guanidinium isothiocyanate, 5-50mM of Tris-HCl with a pH value of 3.5-5.5, 5-25% of Ttiton-X 100, 5-20mM of Urea, 5-20mM of EDTA and 5% of Tween20, the regulated binding liquid is isopropanol, and the deproteinized liquid comprises 2-8M of guanidine hydrochloride, 5-50mM of Tris-HCl with a pH value of 6.5-8.0 and 5-50% of absolute ethyl alcohol. The DNA extracted by the kit and the method has high content and purity, the extraction speed is high, and no toxic reagent is used. The kit and the method are applicable for extracting DNA from various micro samples.

The present invention relates generally to the field of molecular biology and concerns a method for improving various plant growth characteristics by modulating expression in a plant of a nucleic acid encoding a GRP (Growth-Related Protein). The present invention also concerns plants having modulated expression of a nucleic acid encoding a GRP, which plants have improved growth characteristics relative to corresponding wild type plants or other control plants. The invention also provides constructs useful in the methods of the invention. The GRP may be one of the following: Seed Yield Regulator (SYR), FG-GAP1 CYP90B, CDC27, AT-hook transcription factors, DOF transcription factors and Cyclin Dependent Kinase Inhibitors (CKIs).

A method for quantification of an adeno-associated virus genome, including the steps of (a) preparing a composition containing a sample, at least one primer pair for use in amplification of only a nucleotide sequence contained in inverted terminal repeats of an adeno-associated virus, and an intercalating dye; (b) performing nucleic acid amplification reaction using the composition prepared in the step (a); and (c) detecting an amplified product obtained in the step (b). The present invention is especially useful in the fields of medicine, gene engineering, and biology.

The invention relates to the field of molecular biology and provides Oligo dT primer, wherein Oligo dT primer is a single-stranded DNA molecule containing continuous dT sequences and recognition sites for cutting. The invention further provides a method for constructing a cDNA library on the basis of the Oligo dT primer. The Oligo dT primer is excellent in applicability, can be applied to various different library schemes, and can further accurately locate the initiation site of mRNA molecule in poly A tail. The method for constructing the cDNA library can ensure that all mRNA molecules in a sample can show specificity in the constructed cDNA library.

The present invention relates generally to the field of molecular biology and concerns a method for enhancing various yield-related traits and / or plant growth characteristics in plants by modulating expression in a plant of a nucleic acid encoding a C3H-like polypeptide, or a SPATULA-like (SPT) polypeptide, or an IDI2 (Iron Deficiency Induced 2) polypeptide, or an elF4F-like protein complex subunit, or GR-RBP (Glycine Rich-RNA Binding Protein) polypeptide.; The present invention also concerns plants having modulated expression and / or activity of a nucleic acid encoding a C3H-like polypeptide, or a SPATULA-like (SPT) polypeptide, or an IDI2 (Iron Deficiency Induced 2) polypeptide, or an elF4F-like protein complex subunit, or GR-RBP (Glycine Rich-RNA Binding Protein) polypeptide, which plants have enhanced yield-related traits and / or plant growth characteristics relative to corresponding wild type plants or other control plants. The invention also provides constructs useful in the methods of the invention.

The present invention relates generally to the field of molecular biology and concerns a method for increasing plant yield relative to suitable control plants plants. More specifically, the present invention concerns a method for increasing plant yield comprising increasing expression in a plant of a nucleic acid encoding a Dof (DNA-binding with one finger) domain transcription factor polypeptide. The present invention also concerns plants having increased expression of a nucleic acid encoding a Dof domain transcription factor polypeptide, which plants have increased yield relative to suitable control plants. The invention also provides constructs useful in the methods of the invention.

The invention relates to the field of molecular biology, in particular to an AS-PCR (allele-specific polymerase chain reaction) primer design method, a gene mutation detection method and a kit. The AS-PCR primer design method comprises the following steps: (1), an AS-PCR primer is designed for a target sequence containing a to-be-detected allele mutation region; (2), a competition blocking primer is designed for the AS-PCR primer and adopts oligonucleotide in reverse complement with the AS-PCR primer. According to the gene mutation detection method, the AS-PCR primer and the competition blocking primer have a PCR amplification reaction. The kit comprises the AS-PCR primer, the competition blocking primer, a TaqMan probe, an internal control primer, an internal control probe, polymerase, dNTP (deoxynucleotide) and a buffer solution. The AS-PCR primer design method, the gene mutation detection method and the kit have the advantage that the difference of specificity of the AS-PCR primers on mutation sites due to strong and weak mismatch of the mutation sites is reduced effectively.

The invention belongs to the field of molecular biology, and discloses a barcode preparation method used for individual animal identity identification and / or meat product tracing and application thereof. The method comprises the following steps of selecting single nucleotide polymorphism (SNP) sites with high heterozygosity on an animal to be identified or a meat product genome DNA to be traced and combining the SNP sites into an SNP barcode, and then using ten Arabic numbers 0-9 to randomly and uniquely replace the ten SNP gene types: A / A, T / T, G / G, C / C, A / T, A / G, A / C, T / G, T / C and G / C in the SNP barcode, forming corresponding numerical barcodes, and realizing correspondence of individual animal identities and barcodes one by one. According to the method provided by the invention, as a polymerase chain reaction (PCR) primer is designed, the SNP sites with high heterozygosity can be obtained, and the sites can be used for preparing the SNP barcode and / or corresponding numerical barcode used for identifying the individual animal identity and / or tracing the meat product, thereby realizing individual animal identity identification and meat product tracing.

The present invention relates to the fields of chemistry and biology and more particularly to the field of biomaterials. The present invention includes amphiphilic fibers and membranes, which can be used for biomembranes and biocompatible devices. The present invention also relates to processes for preparing amphiphilic fibers and membranes from solutions comprising amphiphilic molecules. More particularly, the present invention relates to processes for preparing fibers and membranes from electrospinning solutions comprising amphiphilic molecules. The present invention further provides fibers and nonwoven membranes comprising amphiphilic fibers chosen from anionic surfactants, cationic surfactants, nonionic surfactants, phospholipids, sulfobetaines, lyotropic liquid crystalline molecules, and / or block copolymers. Electrospun fibers offer the potential for direct fabrication of biologically based, high-surface-area membranes without the use of multiple synthetic steps, complicated electrospinning designs, or post-processing surface treatments. Polymeric phospholipids, for example, have been shown to be attractive candidates for blood purification membranes, artificial heart valves and organs, and other prosthetics, including other biocompatible devices.

The present invention relates generally to the field of molecular biology and concerns a method for enhancing various economically important yield-related traits in plants. More specifically, the present invention concerns a method for enhancing yield-related traits in plants by modulating expression in a plant of a nucleic acid encoding an ASPAT (Asparatate AminoTransferase) polypeptide. The present invention also concerns plants having modulated expression of a nucleic acid encoding an ASPAT polypeptide, which plants have enhanced yield-related traits relative to control plants. The invention also provides hitherto unknown ASPAT-encoding nucleic acids and constructs comprising the same, useful in performing the methods of the invention. Furthermore, the present invention relates generally to the field of molecular biology and concerns a method for increasing various plant yield-related traits by increasing expression in a plant of a nucleic acid sequence encoding a MYB91 like transcription factor (MYB91 ) polypeptide. The present invention also concerns plants having increased expression of a nucleic acid sequence encoding an MYB91 polypeptide, which plants have increased yield- related traits relative to control plants. The invention additionally relates to nucleic acid sequences, nucleic acid constructs, vectors and plants containing said nucleic acid sequences. Even furthermore, the present invention relates generally to the field of molecular biology and concerns a method for improving various plant growth characteristics by modulating expression in a plant of a nucleic acid encoding a GASA (Gibberellic Acid-Stimulated Arabidopsis). The present invention also concerns plants having modulated expression of a nucleic acid encoding a GASA, which plants have improved growth characteristics relative to corresponding wild type plants or other control plants. The invention also provides constructs useful in the methods of the invention. Yet furthermore, the present invention relates generally to the field of molecular biology and concerns a method for enhancing various economically important yield-related traits in plants. More specifically, the present invention concerns a method for enhancing yield-related traits in plants by modulating expression in a plant of a nucleic acid encoding an AUX / IAA (auxin / indoleacetic acid) polypeptide. The present invention also concerns plants having modulated expression of a nucleic acid encoding IAA polypeptide, which plants have enhanced yield-related traits relative to control plants. The invention also provides constructs comprising AUX / IAA-encoding nucleic acids, useful in performing the methods of the invention.

The present invention relates generally to the field of molecular biology and regards various polynucleotides, polypeptides and methods that may be employed to enhance yield in transgenic plants. Specifically the transgenic plants may exhibit any one of the traits consisting of increased yield, increased tolerance to abiotic stress, increased cell growth and increased nutrient use efficiency.

Relating to the field of cell biology, the invention discloses a mesenchymal stem cell stock solution, which comprises glucose, sodium chloride, albumin, propylene glycol, cholesterol, and sodium bicarbonate, etc., and also can contain heparin. The mesenchymal stem cell stock solution provided in the invention can realize storing a mesenchymal stem cell at a temperature of -20-4DEG C for 14-20 days and maintaining a motility rate of over 85%, thus being convenient for cell transport and temporary storage. Also, the stock solution of the invention has components all coming from medicinal injectable reagents that are safe for human bodies, and can be injected directly after unfreezing, thus providing great convenience for long distance supply and clinical application of mesenchymal stem cell preparations.