5 results about "Chinese population" patented technology

The invention relates to the technical field of forensic genomics, in particular to a primer group and a kit for detecting genetic markers of 365 Y chromosomes and application of the primer group and the kit. According to the invention, an amplification primer group for specific 57 Y-STRs and 308 Y-SNPs of Chinese population is optimally designed, and a joint detection method with high sensitivity and high resolution is constructed. Y-STR haplotype analysis and Y-SNP haplogroup accurate typing can be synchronously realized, and the problems of high family checking false positive rate, insufficient geographic ancestor inference resolution and the like caused by single site type detection in the traditional technology are solved. The method is suitable for diversified biological samples such as blood, seminal stains and saliva, and provides a novel technical scheme of high-throughput, low-cost and multi-dimensional paternal genetic information analysis for forensic practice. Comprising construction of a Y-STR and Y-SNP composite amplification system based on next-generation sequencing and application of the Y-STR and Y-SNP composite amplification system in forensic medicine individual recognition, family investigation and biogeography ancestor inference.

This invention discloses a reagent, detection system, and method for HPA genotyping in the Chinese population. The invention employs a multiplex PCR technique based on fluorescent probe melting curves, arranging and combining specific primers and fluorescently labeled probes for different HPA systems. This ultimately enables simultaneous genotyping of 14 HPA antigen systems in the Chinese population using only three reaction tubes in a single test. Furthermore, the detection system of this invention is not only simple and rapid but also accurate and efficient, possessing high practical value.

This invention belongs to the field of genetics and provides a technique and data analysis method for simultaneously detecting 193 Y-STRs in the Chinese population based on an NGS platform. This invention discloses 193 Y-STR loci for human Y-chromosome STR genotyping; the selected 193 Y-STRs are used for library construction using a two-step PCR method. This invention has advantages such as rapid amplification, consistent efficiency, high sensitivity, and good data visualization; due to the high depth of high-throughput sequencing, it is more conducive to the analysis of mixed and degraded materials.

The invention discloses a Chinese population POPs lifetime cumulative risk assessment method and system. The method comprises the following steps: S1, big data collection and preprocessing; s2, constructing an age and gender related dynamic relation model; s3, optimizing the POPs human body accumulation model; s4, carrying out POPs removal half-life period fitting on the Chinese population; s5, carrying out life-long cumulative trend simulation and risk assessment on the POPs; the system comprises a data acquisition module, a dynamic relation modeling module, an accumulation model construction module, a model calibration verification module and a risk assessment module. The method and system can solve the problems that an existing model is complex, poor in applicability and incapable of adapting to Chinese population characteristics and full-life-cycle evaluation requirements, achieve accurate simulation and prediction of the lifelong and intergenerational POPs accumulation level of Chinese population, provide a scientific tool for population health risk evaluation and environment management policy making, and improve the economic benefits of people. Good popularization and application prospects are realized.

This invention discloses a primer set, kit, and application for prenatal screening of RhD, belonging to the field of gene detection technology. Based on genetic data of the Chinese population, this invention proposes four specific primer pairs. Through specific amplification, it can effectively distinguish four genes: RhD complete deletion gene, RhD-CE(2-9)-D hybrid gene, RhD1227A, and RhD wild-type gene. Compared with existing technologies, by incorporating RhD exon9 amplification, it can detect the RhD1227A gene, which accounts for approximately 16.3% to 32.6% of RhD-negative blood types in the Chinese population, reducing the probability of false positives due to paternal RhD1227A gene carriage. This provides a more accurate, low-cost, rapid, and convenient option for prenatal screening of RhD in the Chinese population.