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3566 results about "Genotype" patented technology

The genotype is the part of the genetic makeup of a cell, and therefore of any individual, which determines one of its characteristics (phenotype). The term was coined by the Danish botanist, plant physiologist and geneticist Wilhelm Johannsen in 1903.

Molecular marker influencing sheep weight traits and application thereof

The invention belongs to the technical field of genetic breeding, and particularly relates to a molecular marker influencing sheep weight traits and application thereof, and the molecular marker comprises a site I or a site II. The nucleotide sequence of the molecular marker containing the site I is as shown in SEQ ID NO.1, and mutation from T to C occurs at the 101bp position in the SEQ ID NO.1; the nucleotide sequence of the molecular marker containing the site 2 is as shown in SEQ ID NO.2, and mutation from A to G occurs at the 101bp position in the SEQ ID NO.2. According to the molecular markers influencing the sheep weight traits, the marker I and the marker II can be used for identifying the sheep weight traits, and the offspring weight can be increased by selecting TT or AA genotype individuals as male parents or female parents.
Owner:INNER MONGOLIA AGRICULTURAL UNIVERSITY

Molecular marker related to salt-tolerant and rapid growth traits of grass carp and application of molecular marker

The invention provides a molecular marker related to salt-tolerant rapid growth traits of grass carps and application of the molecular marker, and belongs to the technical field of molecular markers. According to the invention, a group of molecular markers related to salt-tolerant and rapid growth traits of grass carp are obtained through GWAS analysis and screening. The invention also develops a group of amplification primers based on the molecular marker, the amplification primers can realize screening and / or identification of salt-tolerant fast-growing grass carp germplasm, and the more the enriched dominant genotypes are, the higher the growth trait index is. Compared with the prior art, the molecular marker provided by the invention can be used for accurately and efficiently identifying high-quality grass carp individuals with salt-tolerant and fast-growing characters, and the salt-tolerant breeding progress of grass carp is accelerated.
Owner:FRESHWATER FISHERIES RES CENT OF CHINESE ACAD OF FISHERY SCI

Larimichthys crocea medium-low density SNP liquid phase chip and application thereof

The invention provides a large yellow croaker medium-low density SNP liquid phase chip and application thereof, the chip is prepared from a probe for detecting 15,292 SNP molecular marker combinations of a large yellow croaker whole genome, and the position and base information of each SNP site on the chip are shown in a table 1. The invention further provides a preparation method and application of the chip, such as application in large yellow croaker population genetic diversity analysis, breeding population genotype identification, character correlation analysis or genome selective breeding. The chip designed by the invention comprises a series of core sites obviously related to growth, flow resistance and quality characters, and also comprises a large number of background sites, so that the detection accuracy of the designed chip is guaranteed, and the chip has the obvious advantage of low detection cost, the detection cost is reduced by more than 30%, and the detection efficiency is improved. The scientific and technological innovation of the large yellow croaker dark blue breeding industry can be further accelerated.
Owner:EAST CHINA SEA FISHERIES RES INST CHINESE ACAD OF FISHERY SCI

Sugarcane germplasm resource evaluation and breeding method for smart agriculture

The invention discloses a sugarcane germplasm resource evaluation and breeding method for smart agriculture. The sugarcane germplasm resource evaluation and breeding method comprises the following steps: step 1, collecting materials with wide genetic diversity; establishing an intelligent incubator and a greenhouse, and monitoring environmental parameters by using an Internet of Things sensor; 2, performing data acquisition on the related germplasm resources by using an unmanned aerial vehicle, a robot and a near infrared spectrum technology, and constructing a phenotype database for data management; meanwhile, genotype identification is carried out by combining molecular marker-assisted selection and genome selection technologies, molecular markers of key genes are mined, and the genetic value of related germplasm is evaluated; step 3, constructing a hybrid combination prediction model by using an AI algorithm, optimizing parent matching of the related germplasm and the cultivated sugarcane, monitoring and collecting the filial generation at the same time, and analyzing and screening in combination with smart agriculture; 4, demonstration planting is conducted on the bred excellent sugarcane strain, and application of the excellent strain is improved through government-enterprise-scientific research institution collaborative popularization and in combination with farmer technical training.
Owner:YUNNAN AGRICULTURAL UNIVERSITY

Molecular marker for auxiliary screening of anti-freezing wheat germplasm and application thereof

The invention discloses a molecular marker for auxiliary screening of anti-freezing wheat germplasm and application of the molecular marker, and relates to the technical field of biology, in particular to a method for screening or auxiliary screening of wheat with different freezing resistance, which comprises the following steps: detecting whether the genotype of wheat to be detected is qFDG-7A. 3a or qFDG-7A. 3b, and the freezing resistance gt of the wheat with the genotype qFDG-7A. 3a; the freezing resistance of the wheat with the genotype qFDG-7A. 3b; the wheat of which the genotype is qFDG-7A. 3a is wheat of which the genotype is AA homozygous based on an A129C SNP (Single Nucleotide Polymorphism) site; the wheat of which the genotype is qFDG-7A. 3b is wheat of which the genotype is CC homozygous based on an A129C SNP (Single Nucleotide Polymorphism) site; the A129C SNP site is the 129th nucleotide from the 5'tail end of SEQ ID NO: 1 in a wheat genome. The molecular marker has important theoretical significance and economic value for molecular marker-assisted selection of wheat germplasm or breeding progeny materials with high anti-freezing capacity.
Owner:INST OF CEREAL & OIL CROPS HEBEI ACAD OF AGRI & FORESTRY SCI

Molecular marker for detecting gibberellic disease resistant QTL QFhb-yaas-4AL and application

The invention discloses a molecular marker for detecting a gibberellic disease resistant QTL (Quantitative Trait Loci) QFhb-yaas-4AL and application of the molecular marker. Genotype data are obtained by utilizing a Wheat16K wheat high-throughput gene chip, a QTL locus QFhb-yaas-4AL which is derived from a resistant material and is related to the resistance of the gibberellic disease is detected, and a KASP marker and a detection primer group thereof are developed and are used for efficiently screening the resistance of the gibberellic disease. The primer group disclosed by the invention is used for performing PCR amplification on wheat genome DNA, whether a gene with high gibberellic disease resistance of a resistant material is carried or not can be directly judged through KASP typing, the detection method is simple to operate, the detection result is very intuitive, the detection effect is obvious and effective, and the primer group can be widely applied to the field of genetic engineering. When the molecular marker is used for screening, the working efficiency of molecular marker-assisted selection of wheat breeding with high and low gibberellic disease resistance can be greatly improved.
Owner:JIANGSU LIXIAHE REGION AGRI RES INST

SNP-C1216T related to thousand seed weight of wheat and application of SNP-C1216T

The invention discloses a wheat thousand seed weight related SNP-C1216T and application thereof, the SNP site corresponds to the 1216th basic group from the 5'terminal of the sequence shown in SEQ ID NO.1, and when the site is C / C homozygous, the corresponding genotype is A; when the locus is T / T homozygous, the corresponding genotype is B; the thousand seed weight of the wheat with the homozygous genotype A is greater than or candidate to be greater than that of the wheat with the homozygous genotype B. The SNP has high effectiveness and potential application value, wheat with high thousand seed weight can be found by detecting the SNP, and the SNP has important value in research or application of cultivation of high-yield wheat varieties.
Owner:SHIJIAZHUANG INST OF AGRI MODERNIZATION CHINESE ACAD OF SCI

Porcine muscle stem cell line capable of being cultured in vitro for long time and application of porcine muscle stem cell line

The invention discloses a porcine muscle stem cell line capable of being cultured in vitro for a long time and application of the porcine muscle stem cell line, and belongs to the technical field of stem cells and cell culture meat. The porcine muscle stem cell line is prepared by the following steps: performing CDKN2A gene knockout on porcine muscle stem cells by using a CRISPR / Cas9 gene editing technology, performing monoclonal screening and genotype identification to obtain a cell strain with successful CDKN2A knockout, and then performing carrier-free and serum-free suspension culture domestication on the cell strain. And a carrier-free and serum-free porcine muscle stem cell line capable of being subjected to in-vitro long-term suspension culture is obtained. Verification shows that the CDKN2A knockout porcine muscle stem cell line subjected to carrier-free and serum-free suspension culture domestication not only can be subjected to carrier-free and serum-free suspension culture in a shake flask for a long time, but also can maintain a good differentiation characteristic. The porcine muscle stem cell line which can be cultured on a large scale, is derived from edible animals and can be cultured in vitro for a long time is created, and excellent cell resources are provided for commercial production of cultured meat.
Owner:NANJING JOES FUTURE FOOD TECH CO LTD

Methods and systems for characterizing morphodynamic profiles of objects

This disclosure provides a novel method and system for characterizing morphodynamic profiles of objects, such as biological entities. This disclosure provides a shape, appearance, and motion (SAM) phenotype Observation Tool (SPOT). SPOT establishes a standardized SAM “phenome,” image descriptors resembling single-cell transcriptomes, to comprehensively quantify a cell's instantaneous state without prior knowledge. SPOT also establishes a standardized workflow for temporal analysis. SPOT is a generalist tool, applicable to any live-cell imaging and advances biomedical discovery through its standardized, unbiased, streamlined workflow to quantify phenotypic heterogeneity and predict phenotype-genotype-function coupling.
Owner:THE CHANCELLOR MASTERS AND SCHOLARS OF THE UNIVERSITY OF OXFORD

STON1 gene molecular marker primer related to chicken double-yolk egg traits and application of STON1 gene molecular marker primer

The invention relates to an STON1 gene molecular marker primer related to chicken double-yolk egg traits and application of the STON1 gene molecular marker primer, and belongs to the technical field of biology. The STON1 gene molecular marker is a 7867409th basic group of a chicken reference genome GRCg7b version chromosome 3, the basic group is mutated into A or G, and the genotypes are A / A, A / G and G / G. The double-yolk egg ratio of the G / G genotype chicken is higher than that of the A / G and A / A genotype individuals, and the double-yolk egg ratio of the A / G genotype chicken is higher than that of the A / A genotype individuals. A forward and reverse primer pair is used for amplifying a fragment containing the molecular marker site, Sanger sequencing is carried out on an amplification product, the genotype of an individual is rapidly and accurately identified to serve as a genetic marker for chicken breeding, and the chicken with the higher proportion of double-yolk eggs is bred; the double-yolk egg traits can be efficiently and accurately detected, the breeding accuracy and breeding efficiency of the chicken double-yolk egg proportion are improved, and the method has important value for chicken breeding and production.
Owner:NANJING AGRICULTURAL UNIVERSITY

SNP (Single Nucleotide Polymorphism) molecular marker related to conception rate of young cow, detection product and application of SNP molecular marker

The invention discloses an SNP molecular marker related to the conception rate of young cows, a detection product and application of the SNP molecular marker, and belongs to the technical field of dairy cow breeding markers. The SNP molecular marker is chr6: g. 103355581Cgt, and the SNP molecular marker is a molecular marker. The method comprises the following steps of: taking a bovine reference genome version UMD3.1 as a standard for a coordinate position, numbering Assessment in NCBI (National Center of Biotechnology Information) as GCF000003055.6, and numbering a chromosome as AC 000163.1; the young cows are cows which are 12-24 months old and do not lay calves. By detecting the genotype of the SNP site, the conception rate condition can be effectively judged, parent individuals with the higher young cow conception rate can be screened, early selection of genotype individuals with the high young cow conception rate is achieved, the frequency of the genotype with the high young cow conception rate in a dairy cow group is increased, and the reproduction rate of the dairy cow group is increased; the service life of cows is prolonged, the treatment cost is saved, the breeding cost is reduced, and the pasture income is increased.
Owner:INST OF ANIMAL SCI & VETERINARY MEDICINE SHANDONG ACADEMY OF AGRI SCI

Cynoglossus semilaevis vibrio harveyi-resistant KASP molecular marker combination and application thereof in disease-resistant breeding of cynoglossus semilaevis

The invention belongs to the technical field of aquatic biotechnology and molecular marker-assisted breeding, and particularly relates to a cynoglossus semilaevis vibrio harveyi-resistant KASP molecular marker combination and application thereof in cynoglossus semilaevis disease-resistant breeding. The KASP molecular marker combination comprises three SNP (Single Nucleotide Polymorphism) loci which are respectively positioned at 17314099, 17325496 and 17355989 loci of the 14 # chromosome of the cynoglossus semilaevis, when the genotypes of the three SNP loci are respectively CC, AA and GG, the disease resistance of the cynoglossus semilaevis is high, and the invention also provides a primer for detecting the genotype polymorphism of the SNP loci. By utilizing the KASP molecular marker combination, the vibrio harveyi resistance genotype in a cynoglossus semilaevis breeding population can be quickly detected, and disease-resistant individuals are screened, so that the disease-resistant breeding efficiency of the cynoglossus semilaevis is greatly improved, and a new variety of the cynoglossus semilaevis with strong disease resistance can be quickly cultivated.
Owner:YELLOW SEA FISHERIES RES INST CHINESE ACAD OF FISHERIES SCI

Monoclonal antibody for detecting human metapneumovirus, detection reagent and application thereof

The invention relates to a monoclonal antibody for detecting human metapneumovirus, a detection reagent and application of the monoclonal antibody and the detection reagent. The monoclonal antibody or the antigen binding fragment thereof can be specifically bound with the human metapneumovirus N protein with higher affinity, has broad-spectrum effectiveness on various genotypes, and has no cross reactivity with other respiratory viruses, so that the monoclonal antibody or the antigen binding fragment thereof has huge potential application value in clinical detection of human metapneumovirus infection.
Owner:CAPITAL INST OF PEDIATRICS

KASP molecular marker, primer pair and kit for identifying dwarf character of muskmelon and application of KASP molecular marker, primer pair and kit

The embodiment of the invention discloses a KASP molecular marker, a primer pair and a kit for identifying the dwarf character of muskmelon and application of the KASP molecular marker, relates to the technical field of molecular markers, and aims to promote gene positioning and molecular-assisted breeding processes by utilizing molecular marker-assisted selective breeding on the dwarf character of muskmelon. The method for identifying the dwarf character of the muskmelon comprises the following steps: extracting genome DNA of a target muskmelon, taking the genome DNA of the target muskmelon as a template for PCR amplification, and performing fluorescence detection based on a primer of a KASP marker K1069930 to obtain a gene type of the target muskmelon, on the basis of the detected gene type, the character of the target muskmelon is judged; the KASP marker K1069930 is designed according to an SNP (Single Nucleotide Polymorphism) site at Chr07: 1069930, and the nucleotide sequence of the SNP site is shown as SEQ ID NO. 1. The invention also discloses a kit for detecting the KASP marker K1069930 and a kit for detecting the KASP marker K1069930. The identification method provided by the invention can be more efficiently applied to breeding work of dwarf muskmelon germplasm.
Owner:SANYA PEARL MELON & WATERMELON DISPLAY & EVALUATION RES CENT

KASP molecular marker for identifying soybean hundred-grain weight and application

The invention discloses a KASP molecular marker for identifying soybean hundred-grain weight and application thereof, and relates to the technical field of biology and application of an SNP locus, the SNP locus takes a soybean Wm82. A2. V1 genome sequence as a reference genome, the SNP is the 16321243th SNP on a soybean 11 # chromosome, and the SNP is the 16321243th SNP on the soybean 11 # chromosome. Corresponding to the 20th basic group from the 5'terminal of the sequence shown in SEQ ID NO: 1, when the locus is CC homozygosis, the corresponding genotype is A; when the locus is TT homozygous, the corresponding genotype is B; according to the application, soybeans with different hundred-grain weights are screened or screened in an auxiliary mode, and the different hundred-grain weights of the soybeans are as follows: the homozygous soybeans with the genotype A are higher than or candidate to be higher than the homozygous soybeans with the genotype B. The molecular marker has important theoretical significance and economic value for molecular marker-assisted selection of soybean germplasm or breeding progeny materials with high hundred-grain weight.
Owner:INST OF CEREAL & OIL CROPS HEBEI ACAD OF AGRI & FORESTRY SCI

Rice genotype and phenotype association prediction method based on machine learning

The invention relates to the field of bioinformatics and machine learning, in particular to a rice genotype and phenotype association prediction method based on machine learning. The method comprises the following steps: collecting genotype data and phenotype data of a rice sample, and generating genotype features by adopting a dynamic feature extraction algorithm so as to obtain a genotype feature set; and S2, constructing a genotype and phenotype association prediction model, and predicting phenotypes based on the genotype feature set. The problems that feature information of genotype data is difficult to accurately capture due to non-uniform data distribution or periodic change of different gene loci, and complex features are difficult to recognize by an existing feature extraction method are solved; in the aspect of agricultural genotype and phenotype prediction, especially in the fields of rice breeding, disease resistance enhancement and yield optimization, an effective method special for bioinformatics feature extraction and correlation prediction is lacked; the technical problem of excessive dependence on some unimportant or redundant features due to insufficient performance during feature redundancy processing is solved.
Owner:WEIFANG UNIV OF SCI & TECH +1

Construction method of agrobacterium tumefaciens-mediated masson pine genetic transformation system

The invention discloses a construction method of an agrobacterium tumefaciens mediated masson pine genetic transformation system, and belongs to the technical field of genetic engineering. According to the construction method of the agrobacterium tumefaciens-mediated masson pine genetic transformation system, immature masson pine cones are collected, embryogenic calluses are induced and cultured, the embryogenic calluses of the masson pine in a PEM III stage are used as a material, suspension pre-culture is performed to obtain an acceptor material, and the acceptor material is subjected to genetic transformation to obtain the agrobacterium tumefaciens-mediated masson pine genetic transformation system. The method comprises the following steps: firstly, carrying out tissue culture on a transgenic somatic embryo, infecting by adopting an agrobacterium infection solution with an OD600 value of 0.2-0.6, carrying out co-culture, recovery culture and screening culture, carrying out histochemical staining by adopting GUS, carrying out molecular identification to obtain a transgenic embryogenic callus, and carrying out maturation treatment to finally obtain the transgenic somatic embryo. The masson pine genetic transformation system established by the invention is stable in transformation performance and high in transformation positive rate, and when an acceptor material is masson pine embryonic callus with the genotype of 1-3-5, the transformation positive rate is as high as 100%.
Owner:NANJING FORESTRY UNIV

CDK5RAP3 gene SNP molecular marker related to feed conversion rate of yellow feather broilers and application of CDK5RAP3 gene SNP molecular marker

The invention discloses a CDK5RAP3 gene SNP molecular marker related to the feed conversion rate of yellow feather broilers and application of the CDK5RAP3 gene SNP molecular marker, and belongs to the field of animal genetic breeding and molecular biology. The polymorphism of the SNP molecular marker is A / G, the SNP molecular marker is located at the 369307 site of a chicken chromosome 27, and the RS number of the SNP molecular marker is rs7383324012. The invention provides the SNP molecular marker related to the feed conversion rate of the yellow-feathered broiler chicken and located in the CDK5RAP3 gene of the 27th chromosome of the chicken, and the marker shows that the feed conversion rates of the yellow-feathered broiler chickens with different genotypes are obviously different, so that the SNP molecular marker can be used for efficiently screening individual or parent yellow-feathered broiler chickens with excellent characters of low feed conversion rate; the yellow-feather broiler chicken with low feed conversion rate can be quickly bred by applying the yellow-feather broiler chicken feed additive to breeding of the yellow-feather broiler chicken, the feed consumption in the production process can be effectively reduced, and the economic benefits and competitiveness of enterprises are improved.
Owner:SOUTH CHINA AGRICULTURAL UNIVERSITY

Molecular marker related to sheep meat traits and fatty acid content and application of molecular marker

The invention provides a molecular marker related to sheep meat quality traits and fatty acid content and application thereof, and relates to the technical field of biologication.The application comprises the step that substances for detecting SNP sites are applied to preparation of products for predicting the sheep meat quality traits and the fatty acid content. The sheep with the specific genotype at the SNP site has high meat quality character or / and high fatty acid content, and the SNP molecular marker related to the meat quality character and the fatty acid content of the sheep can be used for predicting the meat quality character and the fatty acid content of the sheep, screening sheep individuals with the high meat quality character and the high fatty acid content, and improving the sheep breeding efficiency. The invention further discloses a breeding method for obtaining sheep with high meat quality characters and high fatty acid content, and the breeding speed of dominant sheep is increased.
Owner:JILIN ACAD OF AGRI SCI

HNRNPA2B1 gene-based SNP molecular marker related to bovine sperm malformation rate and application of HNRNPA2B1 gene-based SNP molecular marker

The invention discloses an HNRNPA2B1 gene-based bovine sperm malformation rate related SNP molecular marker and application thereof, and belongs to the technical field of genetic molecular biology, the number of the SNP molecular markers is two, SNP1 is at the position of a bovine genome chr4: 69733358, the polymorphism is C or A, SNP2 is at the position of a bovine genome chr4: 69733387, and the polymorphism is T or G. According to the two SNP loci provided by the invention, the dominant genotypes of g.-1713A > C and g.-1684G > T are AA and GG respectively, and the sperm collection quantity and the sperm malformation rate of the two SNP loci have better performance. By detecting the polymorphism of the two SNP loci, the motility evaluation of the bull sperms can be realized, the breeding is assisted, a reliable support is provided for breeding a high-quality next generation, and the method has the advantages of high reliability, high efficiency, low cost and the like.
Owner:INST OF ANIMAL SCI & VETERINARY MEDICINE SHANDONG ACADEMY OF AGRI SCI +1

Production management system and method based on intelligent production line

The invention discloses a production management system and method based on an intelligent production line, which are suitable for a tobacco process production line, and the method comprises the following steps: collecting genotype information and physicochemical indexes of tobacco raw materials; based on a small sample expansion algorithm and a neural network model of transfer learning, performing process matching analysis on the genotype information to generate an initial processing parameter set; in the cut tobacco making process, the Maillard reaction process is scanned and monitored in real time through a transmission-type THz-TDS probe array, the terahertz spectrum characteristic peak intensity is obtained, microwave auxiliary parameters are dynamically adjusted according to the characteristic peak, and a reaction optimization instruction set is generated; performing aroma constraint verification on the initial processing parameter set and the reaction optimization instruction set, and outputting a feasible process scheme; and executing the feasible process scheme, collecting finished product quality information, and updating the neural network model. According to the method, dynamic optimization and accurate regulation and control of the tobacco primary processing process are achieved, and the product quality stability and aroma type consistency are effectively improved.
Owner:LONGYAN UNIV

Method for predicting whole-growth-period image based on crop genotypes of artificial intelligence

The invention discloses a method for predicting images in a whole growth period based on crop genotypes of artificial intelligence. The method comprises the steps of collecting crop images, processing the crop images, constructing a multi-layer perceptron neural network and a condition control generative adversarial network for training so as to learn the mapping relation of crop genotypes, phenotypes and images in the whole growth period, obtaining a crop gene-phenotype prediction model and a phenotype-image prediction model, and finally obtaining the crop genotype-phenotype prediction model and the phenotype-image prediction model through the two models. Based on the genotype of a to-be-predicted crop, a visual result of the whole growth period of the crop in an ideal environment under the regulation of the gene is predicted. According to the method, based on an artificial intelligence technology, a multi-dimensional space multi-layer perception network and a condition control generative adversarial network are constructed, and vivid and accurate visual prediction of crops is realized by deeply utilizing mapping rules contained in crop genes, phenotypes and image big data.
Owner:HUAZHONG AGRI UNIV

Improved genetic transformation by improving de-novo shoot regeneration in adult plants and during in-vitro tissue culture

Disclosed is an innovative method for genetic transformation through injection of Agrobacterium containing plasmid DNA expressing PLETHORAs (PLT5) or / and WOUNDINDUCED DEDIFFERENTIATION 1 (WIND1) in multiple plant species including Snapdragon and tomato. In addition, this plasmid has also been tested that it can significantly improve the genetic transformation method is not genotype dependent. Genetic transformation can be achieved by promoting de-novo regeneration of shoots in adult plants, which will of significant impact on some perennial plant species such as citrus and blue berry etc. The transgenic de-novo shoot regenerated from adult plants can by-pass the juvenile phase of plants regenerated through the tissue culture method. Expression of PLT5, WIND1, or both can also greatly improve plant regeneration and transgenic transformation through in-vitro tissue culture in recalcitrant plant species.
Owner:UNIV OF FLORIDA RESEARCH FOUNDATION INC

Adenosine triphosphatase sarcoplasmic / endoplasmic reticulum Ca2+ transporting 2 (ATP2A2) gene-based molecular marker for identifying chicken feed efficiency trait, and identification method and use thereof

Provided are an adenosine triphosphatase sarcoplasmic / endoplasmic reticulum Ca2+ transporting 2 (ATP2A2) gene-based molecular marker for identifying a chicken feed efficiency trait, and an identification method and use thereof. A nucleotide sequence of the molecular marker is shown in SEQ ID NO: 1, and a base at locus 926 in the nucleotide sequence is T or G. In the present application, by identifying a genotype of the molecular marker in a chicken genome and then selecting the chicken feed efficiency trait according to the genotype, a breeding method for early selection of the poultry feed efficiency trait is established. The method is simple, rapid, and low-cost, does not require special instruments, and is suitable for the needs of marker-assisted selection experiments.
Owner:ANHUI AGRICULTURAL UNIVERSITY

SNP (Single Nucleotide Polymorphism) molecular marker related to drought resistance of corn and application of SNP molecular marker

The invention discloses an SNP molecular marker related to drought resistance of corn and application of the SNP molecular marker, and relates to the field of agricultural molecular biology. The SNP molecular marker comprises at least one of a first SNP molecular marker and a second SNP molecular marker; the first SNP molecular marker is located at a basic group at a 97605381 site of a chromosome 9 of a corn reference genome Zeamas.AGPv4, and the polymorphism of the first SNP molecular marker is C / T; the second SNP molecular marker is located at the basic group of the 97604011 site of the No.9 chromosome of the corn reference genome Zeamas.AGPv4, and the polymorphism is C / T. The SNP molecular marker disclosed by the invention has the characteristics of high specificity, high sensitivity, high resolution and high typing quality; the drought resistance of the corn material can be quickly judged according to the genotype, and the method has wide application universality and can be used for corn breeding.
Owner:HUAZHI RICE BIO TECH CO LTD +1

InDel molecular marker related to size of lychee kernel and application of InDel molecular marker

The invention relates to the technical field of molecular biology, and particularly provides an InDel molecular marker related to the size of a lychee kernel and application of the InDel molecular marker. The InDel molecular marker is located at a site 25610680 of a fifth chromosome of a reference genome GeneBank: GCA019925255.1 of the litchi, and the InDel molecular marker is used for detecting the DNA of the litchi. The genotype of the marker in a pyrokaryotic litchi material is homozygous' GAAAAA / GAAAAA ', and the genotype of the marker in a macrokaryotic litchi material is heterozygous' G---- / GAAAAA 'or homozygous' G---- / G----'. The invention further provides a primer group for detecting the molecular marker, an effective tool is provided for molecular marker-assisted breeding of the size of the lychee kernel, and the method is expected to assist in shortening the breeding period, improving the breeding efficiency and promoting the improvement work of the lychee variety.
Owner:SANYA RESEARCH INSTITUTE OF HAINAN ACADEMY OF AGRICULTURAL SCIENCES (HAINAN EXPERIMENTAL ANIMAL RESEARCH CENTER) +1

QTL related to maize resistance to stalk rot, SNP molecular markers closely linked with QTL, and use of SNP molecular markers

The present invention relates to the technical field of molecular markers for maize resistance to stalk rot and genetic breeding, and in particular, to a QTL related to maize resistance to stalk rot, SNP molecular markers closely linked with the QTL, and a use of the SNP molecular markers. Provided is a QTL related to maize resistance to stalk rot, wherein the QTL is located on maize chromosome 10. Also provided are SNP molecular markers Ks4 and Ks6 closely linked with a QTL site for maize resistance to stalk rot. By detecting the SNP molecular markers, the genotype of a maize material under test can be accurately identified in a high-throughput manner, a material resistant to stalk rot is selected, and phenotype identification and assisted screening of the maize resistance to stalk rot can also be carried out in a seedling stage, thus significantly reducing production cost and significantly enhancing the efficiency of breeding maize resistant to stalk rot.
Owner:NORTHWEST A & F UNIV +1

Molecular marker related to immune traits of male semi-fine wool sheep as well as detection primer and application of molecular marker

The invention provides a molecular marker related to immune traits of elephant male semi-fine wool sheep and a detection primer and application thereof, and belongs to the technical field of molecular assisted breeding. The nucleotide sequence of the molecular marker is shown as SEQ ID NO.1; the molecular marker comprises an SNP site, the SNP site is located at the 117th site of the molecular marker, the mutation basic group of the SNP site is A or G, and the immune globulin content of an individual male semi-fine wool sheep with the genotype of AG or GG at the SNP site is remarkably higher than that of an individual with the genotype of AA. The content of immune globulins IgA, IgG and IgM of an individual elephant male semi-fine-wool sheep can be judged according to the genotype of the molecular marker, the immune traits of the elephant male semi-fine-wool sheep can be rapidly and accurately evaluated, the high-immunity elephant male semi-fine-wool sheep can be screened, the molecular marker has important significance on early-stage auxiliary breeding of the elephant male semi-fine-wool sheep, the breeding period is remarkably shortened, and the breeding cost is reduced. The breeding efficiency is improved.
Owner:INST OF ANIMAL SCI & VETERINARY TIBET ACADEMY OF AGRI & ANIMAL HUSBANDRY SCI

Linked molecular marker of peanut hypocotyl elongation related gene locus, identification method and application of linked molecular marker

The invention belongs to the technical field of crop genetic breeding, and relates to a linked molecular marker of a peanut hypocotyl elongation related gene locus, an identification method and application of the linked molecular marker. The primer pair is C2-15-F / R, and the sequence of the primer pair is as shown in SEQ ID NO. 1 and SEQ ID NO. 2; by designing an InDel marker C2-15, the peanut hypocotyl length character can be quickly distinguished by using a primer to amplify a target band and using polyacrylamide gel electrophoresis detection. The identification method is more accurate and convenient, has good repeatability, can efficiently distinguish the phenotype and genotype of an individual, shortens the experiment process, has the advantages of simple and feasible operation steps and the like, and saves expenditure. The method disclosed by the invention has important application value in the breeding work aspects such as peanut hypocotyl elongation character related gene positioning, genotype typing and later cultivation of excellent peanut varieties.
Owner:QINGDAO AGRI UNIV +1

Intelligent computing platform for animal gene breeding

The invention discloses an intelligent computing platform for animal gene breeding, which adopts RFID electronic ear tags or other biological recognition technologies to carry out individual marking and identity authentication on each breeding pig, collects basic information of the breeding pig in real time, and carries out intelligent computing on the basis of a statistical genetics model in combination with a machine learning algorithm. The method comprises the following steps: collecting phenotypic data and genotypic data, carrying out deep analysis on the collected phenotypic data and genotypic data, calculating a breeding value or a genome breeding value of each breeding pig, designing and applying an optimization algorithm according to a genetic evaluation result, formulating an optimal breeding pig mating plan, and realizing automatic monitoring of pig house environment parameters through an Internet of Things technology. The method comprises the steps of performing intelligent analysis on behaviors and postures of pigs in combination with an AI image recognition technology, developing a matched software system, integrating data analysis results, establishing a database based on a cloud computing architecture, constructing a visual platform containing various charts and reports, and converting complex data into visual and understandable information.
Owner:SHANGHAI JIAYU HUIKANG INTELLIGENT TECH CO LTD