1366 results about "Genotype" patented technology

The invention discloses SSR (simple sequence repeat) multiple PCR (polymerase chain reaction) primers for paternity test of grass carp and application of the SSR multiple PCR primers. The primers comprise 16 pairs of specific primers, and base sequences of the 16 pairs of specific primers are respectively shown as SEQ ID NO.1-32 in sequence. The invention further discloses a fish paternity test kit comprising the SSR multiple PCR primer, an SSR multiple fluorescent PCR method for grass carp paternity test and application of the SSR multiple PCR primer, the kit or the method in grass carp paternity test. According to the kit, 16 pairs of specific SSR primers, multiple fluorescent PCR and a universal amplification primer technology are combined, 16 sites can be detected at a time, universal primers can be repeatedly used, and compared with simple single-site detection, the efficiency is improved, and the cost is reduced; the microsatellite loci contained in the invention are 3-6 basic groups, the allele size interpretation is more accurate, and the accuracy of genotype data is improved.

The invention belongs to the technical field of genetic breeding, and particularly relates to application of a molecular marker for breast circumference character breeding of grassland short-tailed sheep, the molecular marker is at least one of the following (1)-(3): (1) a nucleotide sequence as shown in SEQ ID NO.1, nucleotide at the 101bp site is A or G; (2) a nucleotide sequence as shown in SEQ ID NO.3, wherein the nucleotide at the 101bp site is C or A; (3) a nucleotide sequence as shown in SEQ ID NO.5, wherein the nucleotide at the 101bp site is T or C; the application refers to any one of the following steps: identifying the chest circumference of the grassland short-tailed sheep; the chest circumference of the offspring of the grassland short-tailed sheep is improved. By detecting the genotype of the molecular marker and selecting a dominant genotype individual as a parent, the average chest circumference of offspring can be effectively improved, and a reliable tool is provided for realizing molecular assisted breeding and precise genetic improvement of the chest circumference character of the grassland short-tailed sheep.

The invention relates to the technical field of molecular markers, in particular to an Indel molecular marker related to yellow leaves of a melon seedling growing point and application of the Indel molecular marker. The invention explores a key candidate gene MELO3C016136 for controlling the leaf color of the growth point of the muskmelon seedling, and develops an Indel molecular marker ygp2 for identifying the leaf color change of the growth point of the muskmelon seedling based on the key candidate gene MELO3C016136. The molecular marker ygp2 can be used for identifying the leaf color change character of the growth point of the muskmelon seedling, only one-time PCR amplification and polyacrylamide gel electrophoresis need to be carried out on the DNA of the plant to be detected, the identification result is reliable, the genotype of the target plant can be simply and conveniently identified, and the genotype identification result is completely consistent with the leaf color phenotype of the growth point of the seedling; important technical support is provided for creating new muskmelon germplasm with yellow seedling growing point leaves.

The invention relates to the technical field of genetic breeding, in particular to application of a molecular marker related to daily gain traits of sheep before weaning. The molecular marker is at least one of the following (1)-(2): (1) a nucleotide sequence as shown in SEQ ID NO.1, wherein the nucleotide sequence at the 101bp site is T or C; (2) a nucleotide sequence as shown in SEQ ID NO.3, wherein the nucleotide sequence at the 101bp site is T or C. The application refers to any one of the following (1) and (2): (1) identifying the daily gain of the sheep before weaning; and (2) the daily gain of the offspring of the sheep before weaning is improved. The screened molecular marker influencing the daily gain character of the sheep before weaning can be used for identifying the daily gain character of the sheep before weaning, and the daily gain of offspring before weaning can be improved by selecting a CC genotype individual as a male parent or a female parent.

The invention relates to the technical field of agricultural biological information, in particular to a rape phenotype-genotype correlation analysis system based on deep learning, which comprises a phenotype image acquisition module, an image preprocessing module, an image phenotype feature extraction module, a multi-dimensional phenotype integration module, a deep learning model training module and a correlation analysis module, the image phenotypic feature extraction module creatively fuses a differential geometry theory and a deep learning technology, maps rape phenotypic features from an Euclidean space to a Riemannian manifold space through a multi-scale differential manifold representation network, and calculates feature correlation based on a geodesic distance by using a manifold attention weighting mechanism. The multi-scale geodesic integration module is adopted to realize organic fusion of phenotypic characteristics of different scales, the association analysis module adopts a multi-level linear regression model to analyze key agronomic characters of the rape, an association relationship between phenotypes and genotypes is established, and the phenotypic characteristic extraction accuracy under a complex background is improved by 35.2% compared with that of a traditional method.

The invention provides a closely linked molecular marker primer of a rice brown planthopper resistant major gene Bph56 as well as a marking method and application of the closely linked molecular marker primer. According to the invention, genotypes of F2 individual plants obtained by hybridizing a rice insect-resistant variety C127 (or) and KW (or) are combined with brown planthopper resistance levels of an F3 family for genetic linkage analysis, a resistance major gene Bph56 carried by the insect-resistant variety C127 is identified, and a region of the resistance major gene Bph56 is reduced to a 77kb fragment limited by markers 6M26.278 and 6M26.355 on two sides and is closely linked with a molecular marker 6M26.355. The molecular marker 6M26.355 disclosed by the invention can be used for effectively detecting whether an insect-resistant variety C127 and derived varieties (lines) of the insect-resistant variety C127 contain the major resistance gene locus or not, so that the selection efficiency of the brown planthopper-resistant character is greatly improved, and the brown planthopper-resistant rice variety (line) containing Bph56 is obtained.

The invention discloses a molecular marker closely linked with a cowpea salt tolerance major QTL (quantitative trait loci) and a primer sequence of the molecular marker. The name of the cowpea salt tolerance major QTL is SC300126, a KASP type molecular marker 300126 closely linked with the cowpea salt tolerance major QTL is located at the position of 43816166 bp on a chromosome 11, the marker can be used for amplifying a material to be detected, and whether a sample carries key allelic variation for controlling salt tolerance or not is judged according to the genotype of the marker. The molecular marker and the primer provided by the invention can be used for quickly screening the cowpea salt tolerance major QTL, and compared with a traditional phenotype identification method, the molecular marker and the primer have the advantages of high detection flux, accurate and reliable result and the like, and the typing accuracy can reach 99% or above. The technology can significantly improve the breeding efficiency of the salt-tolerant variety of cowpea and shorten the breeding period.

The invention belongs to the technical field of plant molecular markers, and particularly discloses an Indel molecular marker related to the salt tolerance of brassica napus and application of the Indel molecular marker. The marker is located at the position of an A01 chromosome scafoldA0133077947 of a brassica napus reference genome ZS11.v10, and the genotype of a polymorphic site is CTAA or C. The invention further discloses a method for identifying the marker of the brassica napus. Large-scale group phenotypic association analysis proves that the molecular marker is remarkably associated with the salt tolerance of the brassica napus in the germination period, and when the polymorphic site genotype is CTAA, the salt tolerance in the germination period is high; when the genotype of the polymorphic site is C, low salt tolerance in the germination period is shown. The invention finds that the polymorphic site is obviously associated with the salt tolerance of the brassica napus for the first time, provides a powerful molecular tool for solving the problems of difficulty in phenotype identification, long period and low efficiency in salt tolerance breeding of the brassica napus, can accurately screen salt-tolerant materials in the early stage, and greatly accelerates the breeding process of new varieties of the salt-tolerant brassica napus.

The invention discloses a corn crossbreeding intelligent matching system and method based on artificial intelligence, and the method comprises the steps: collecting molecular marker genotype data and phenotype data of candidate parents, calculating the genetic distance between the parents, and constructing a genetic distance matrix; analyzing the phenotypic complementarity degree of the parent pair, and calculating a phenotypic complementarity index; constructing a heterosis prediction model based on genetic distance and phenotype complementarity, and predicting the yield heterosis value of the candidate hybrid combination; evaluating the environmental adaptability and resistance comprehensive score of the candidate combination; and adopting a Pareto multi-objective optimization strategy to screen an optimal matching scheme. According to the method, molecular marker data and phenotype data are integrated, a heterosis prediction model considering a genetic distance nonlinear effect is constructed, collaborative optimization of multiple breeding targets such as yield, resistance and environmental adaptability is achieved, the prediction accuracy is improved by about 20% compared with that of a traditional method, and the method is suitable for large-scale popularization and application. And an intelligent and precise decision support tool is provided for corn crossbreeding.

The application is suitable for the technical field of germplasm resources, and provides a germplasm resource data management system.The system comprises: an approval and registration sample management system, a resource survey collection management system, a germplasm resource library position management system, a germplasm resource phenotype database and a germplasm resource genotype database, the approval and registration sample management system manages data of crop standard samples; the germplasm resource phenotype database stores phenotype data of crop germplasm resources; the germplasm resource genotype database stores genotype data of crop germplasm resources; the germplasm resource library position management system manages data of crop germplasm resources; the phenotype data of crop germplasm resources, the genotype data of crop germplasm resources and the data of crop germplasm resources are corresponded through library number coding.The application can scientifically manage crop germplasm resource data, and can also realize internet sharing of crop germplasm resource data.

The invention discloses a risk assessment method, system and equipment for idiopathic pulmonary arterial hypertension, and belongs to the field of pulmonary arterial hypertension. According to the method, SNP data containing genotypes and effect values, protein marker expression quantity, metabonomics and clinical data are obtained, the SNP effect values are corrected based on linkage imbalance reference information, and PRS is calculated in combination with the genotypes; constructing a protein expression score by utilizing the site effect value and the expression quantity of the pQTL, and fusing the protein expression score with the PRS to form a target PRS; carrying out dimensionality reduction on metabolome data by adopting sparse coding, extracting sparse coefficients of IPAH related metabolic pathways, and converting the sparse coefficients into metabolic pathway scores; converting the clinical indexes into clinical risk scores; based on the clinical parameter distribution target PRS, the metabolic pathway score and the weight coefficient of the clinical risk score, calculating a risk assessment value; and finally, matching the evaluation value with a preset risk threshold value, and outputting a risk evaluation level. And the IPAH risk assessment accuracy of common people is improved.

The invention relates to the technical field of genetic breeding, in particular to application of a molecular marker related to the weaning weight character of sheep, the molecular marker is a nucleotide sequence shown in SEQ ID NO.1, and nucleotide at the 101bp site is T or C; the application refers to any one of the following (1) and (2): (1) identifying the weaning weight of sheep; and (2) the weaning weight of the offspring of the sheep is improved. The screened molecular marker influencing the weaning weight character of the sheep can be used for identifying the weaning weight character of the sheep, and the weaning weight of offspring can be improved by selecting a CC genotype individual as a male parent or a female parent.

The invention discloses an SNP (Single Nucleotide Polymorphism) molecular marker related to a Hu sheep body height character and application thereof, and belongs to the field of gene engineering. The SNP molecular marker is located at the nucleotide at the 71034104th site of the No.17 chromosome of the sheep reference genome AR-UIRambv3.0 version, the polymorphism of the site is T or C, and the genotype is TT, TC or CC. Correlation analysis verifies that the body height of a TT genotype Hu sheep individual is obviously higher than that of TC genotype and CC genotype individuals. The invention further provides a primer for detecting the SNP molecular marker and application of the primer in Hu sheep body height character auxiliary screening. By utilizing the molecular marker, dominant genotype individuals can be quickly and accurately identified in the young Hu sheep period without waiting for the Hu sheep to grow to the body size for determining the age, the breeding efficiency of the Hu sheep body height can be greatly improved, and the molecular marker has important significance for breeding large Hu sheep strains and improving the breeding economic benefits.

The invention relates to the technical field of molecular markers, in particular to application of a detection reagent of an SNP (Single Nucleotide Polymorphism) molecular marker related to goat meat quality traits. The nucleotide sequence of the SNP molecular marker is as shown in SEQ ID NO.1, the SNP site is located at the 139th site from the 5'end of the sequence as shown in SEQ ID NO.1, the polymorphism of the SNP site is A or G, and the tenderness of longest muscle on the back of a goat with the genotype of AG type or GG type is greater than that of a goat with the genotype of AA type. The invention also develops a reagent for detecting the SNP molecular marker, and the reagent can be used for identifying goat meat traits, so that goat meat which is fresh and tender in meat quality and fine and smooth in mouth feel can be screened out, and the SNP molecular marker has important value in the aspects of goat breeding, assistant breeding or goat meat use value improvement and the like.

The invention discloses a forest tree genotype-environment interaction modeling method based on multi-modal deep learning, and relates to the technical field of forest tree breeding, the method comprises the following specific steps: multi-modal data acquisition: adopting a high-throughput phenotype platform, a whole genome sequencing technology and soil nutrient detection equipment to acquire multi-modal data; sNP locus genotype data, soil key physicochemical index environmental data and growth-related morphology and biomass parameter phenotype data of forest trees are collected respectively; forest genotype, environment and phenotype multi-modal data are collected through the system, the genotype-environment interaction algorithm and the phenotype prediction model are constructed after preprocessing and fusion, the model can accurately predict forest phenotypes, the breeding screening period is remarkably shortened, the breeding selection precision and efficiency are greatly improved, and the method is suitable for large-scale popularization and application. The method effectively solves the problem that a traditional breeding mode is short in time and efficiency, enables breeding work to respond to market demands and environmental changes more quickly and accurately, and provides powerful support for sustainable development of the forestry industry.

The invention relates to the technical field of digital twinborn and agricultural information, and discloses a digital twinborn growth simulation and optimization method for safflower seedling breeding, and the method comprises the following steps: constructing individual digital twinborn bodies fusing genotypes, phenotypes and multi-dimensional environmental perception data; performing hybrid modeling in combination with the mechanism model and a depth time sequence neural network, and correcting growth prediction deviation in real time; and based on multi-objective optimization of biomass, medicinal component content and water efficiency, generating a precise cultivation regulation and control instruction. The system comprises a data acquisition unit, a twin construction unit, an environment fusion unit, a hybrid modeling unit, a visualization unit, an optimization decision unit and an instruction execution unit. According to the method, through closed-loop intelligent regulation and control, the yield, quality and resource utilization efficiency of safflower seedling breeding are remarkably improved.

The invention relates to the technical field of biology, in particular to a molecular marker for identifying Holstein cattle with high fecundity and application of the molecular marker. According to the invention, an RBX1 gene is analyzed in cattle 150K genotype chip data, and SNP loci related to first and last hybridization interval characters are found; the method comprises the following steps: establishing a 30x re-sequencing database of high and low reproduction populations for the first time, and autonomously identifying the SNP sites of the high-fecundity Holstein cattle; an important molecular target is provided for the creation of a new gene editing breeding material in the later period, the creation of an autonomous breeding chip in China and the breeding of cattle.

The invention belongs to the technical field of plant molecular breeding, and particularly relates to a KASP molecular marker related to the oil content of soybeans and application of the KASP molecular marker. The molecular marker is based on a soybean Glyma. 13G035200 gene, and targets an SNP (Single Nucleotide Polymorphism) site, which is remarkably associated with the soybean oil content, in the gene. The invention further provides a specific primer combination and a detection method of the molecular marker, soybean genotypes can be rapidly distinguished through PCR amplification and fluorescence signal detection, and high-oil-content soybean individuals can be accurately screened. The molecular marker has the advantages of being high in specificity, efficient in detection, reliable in result and the like, can be applied to soybean molecular marker assisted breeding, germplasm resource screening and high-oil soybean variety cultivation, remarkably shortens the breeding period, reduces the breeding cost, and provides an efficient tool for precise improvement of the soybean oil content character.

The invention discloses a molecular marker related to slash pine paper pulp, a DNA (deoxyribonucleic acid) fragment, a kit and application of the molecular marker, the SNP molecular marker is S1641460596 and is located at the 641460596 site of a slash pine chromosome 1; the polymorphism of the SNP site is G / A, the genotype of the SNP site of the slash pine with the long wood fiber length is GG, and the genotype of the SNP site of the slash pine with the short wood fiber length is GA or AA. The DNA fragment of the gene is located in a Pel1G156900 gene region of a slash pine genome, and the nucleotide sequence of the gene is as shown in SEQ ID NO. 1 or SEQ ID NO. 2. The kit comprises a forward primer and a reverse primer which are used for amplifying the DNA fragment related to the slash pine paper pulp; the nucleotide sequence of the forward primer is as shown in SEQ ID NO.3, and the nucleotide sequence of the reverse primer is as shown in SEQ ID NO.4; the molecular marker, the DNA fragment and the kit are used for related phenotype identification, early prediction, breeding and the like of slash pine paper pulp, and the problems of late identification, long breeding period, low breeding efficiency and the like of existing slash pine paper pulp characters can be solved.

The invention discloses a structural variation molecular marker located on a pig chromosome 7 and related to the lactation ability of a sow. The structural variation molecular marker is a DNA (deoxyribonucleic acid) fragment which is inserted between the 112024896 bp site and the 112024896 bp site on a chromosome 7 of an international pig reference genome 11.1 version, wherein the nucleotide sequence of the DNA fragment is shown as SEQ ID NO: 1; the genotype of the gene is T / T, T / INS and INS / INS. The structural variation molecular marker provided by the invention obviously influences the total weight gain character and the lactation ability character of the 7-day-old sow, and by breeding the pig with the structural variation molecular marker genotype of T / T, the lactation ability of the sow can be obviously improved, and the survival rate and the weaning weight of piglets are increased.

The invention relates to the technical field of molecular markers, in particular to a molecular marker for early identification of immune traits of elephant male semi-fine wool sheep and application of the molecular marker. According to the present invention, the deterministic correlation between the genotype of the 102308292 site on the second chromosome and the immune globulin (IgA, IgG and IgM) level of the male semi-fine wool sheep is found for the first time; by detecting the genotype of the site, the future humoral immunity of the lamb can be predicted in the lamb stage, so that early seed selection is realized. The invention provides a powerful SNP marker for non-diagnostic purpose for disease-resistant breeding of male semi-fine wool sheep.

The invention belongs to the field of plant biotechnology breeding, and particularly relates to a fine positioning molecular marker closely linked with rape grain weight main effect QTL-qSW.A3 and application of the fine positioning molecular marker. According to the present invention, the rape grain weight QTL-qSW.A3 is finely positioned through the molecular marker, the PARMS marker is adopted to transform and identify the genotype, and the QTL-qSW.A3 explains the average 10% phenotypic variance, and can be used for the genetic improvement of the rape grain weight and the yield;

The invention belongs to the technical field of aquatic product breeding, and particularly relates to a KASP molecular marker related to high temperature resistance character of patinopecten yessoensis and application of the KASP molecular marker in genetic breeding. On the basis of genome re-sequencing of 200 patinopecten yessoensis, a PySNP587919 locus obviously associated with the patinopecten yessoensis high-temperature-resistant character is screened by combining a whole genome SNP genotype and whole genome association analysis of the patinopecten yessoensis high-temperature-resistant character, and after the reliability of the locus is verified through Sanger sequencing, the locus is developed into a KASP marker, and the KASP marker can be used for identifying the high-temperature-resistant character of the patinopecten yessoensis and identifying the high-temperature-resistant character of the patinopecten yessoensis. The screening method can be used for screening the high-temperature-resistant comb shells. Furthermore, the invention also establishes a method for screening the high-temperature-resistant patinopecten yessoensis, and the method is efficient and accurate. Therefore, the technical scheme provided by the invention is suitable for molecular marker-assisted breeding work, rapid and effective screening of parents can be realized by typing the PySNP587919 site, and the breeding efficiency is improved.

Systems and methods are provided that include using models configured to generate offspring genotypes and a fitness evaluator configured to evaluate fitness of the offspring genotypes.

Methods, kits, and systems for assessing the risk of a human subject for developing a cancer, including genetic risk assessment, clinical risk assessment, and combinations of both to improve risk analysis. Technologies utilize, among other things, analyzing a sample of DNA obtained or derived from a subject to detect the genotype for a plurality of test genomic loci.

The invention discloses a pleiotropic InDel molecular marker related to soybean yield, quality and agronomic traits and application, and relates to the technical field of molecular detection. Whole genome association analysis finds that a specific number of TC repetitive sequences exist in a specific region of a No.19 chromosome of soybean and are remarkably related to the characters of soybean yield, quality, plant height, leaf type and flowering time, and an InDel molecular marker is developed based on the TC repetitive sequences. By utilizing the InDel molecular marker, the genotype of a target site can be quickly and accurately detected, and soybean materials with different genetic backgrounds can be distinguished, so that soybean varieties with excellent agronomic traits such as yield, quality related traits, plant height and flowering time can be effectively screened in an auxiliary manner. According to the method, the breeding selection accuracy and efficiency are remarkably improved, and a powerful tool is provided for accelerating the soybean genetic improvement process.

The invention relates to the field of genome prediction, and particularly discloses a GEP-GANGP-based genome prediction system and method, and the method comprises the steps: generating synthetic phenotype data by using GEP-cGAN; processing real and synthetic data through a feature extraction network; calculating interaction characteristics of the genotype and the environment by adopting a cross-modal attention mechanism; a transfer learning module is used for realizing knowledge transfer and feature enhancement; synchronously outputting a phenotype prediction value, an effect weight and an interpretability characteristic through a multi-task decoder; the system comprises a GEP-cGAN module, a biological multi-head attention mechanism BMAM module, a cross-modal interaction feature extraction module, a transfer learning TrG2P module and a multi-task decoder module. According to the method, the problems of low prediction precision, poor generalization ability and weak model interpretability in a small sample scene in forest tree breeding are solved, the accuracy and practicability of genome prediction are remarkably improved, and reliable technical support is provided for forest tree genetic improvement.

The invention discloses an SNP (Single Nucleotide Polymorphism) molecular marker combination related to saline-alkaline tolerance of elymus dahuricus and application of the SNP molecular marker combination. The SNP molecular marker combination is composed of five SNP molecular markers, the SNP molecular markers in the group are located at the 80176348th site of a chromosome 6 of elymus dahuricus, the 490854164 site of a chromosome 9 of elymus dahuricus, the 291625249 site of a chromosome 11 of elymus dahuricus and the 64884340th site and the 65147058 site of a chromosome 12 of elymus dahuricus, base polymorphism CC / CG (GG), TT / TG, AA / TT, AA / GG and TT / CC exist at the sites, CC-TT-AA-AA-AA-TT is a saline-alkaline tolerant genotype, and CG / GG-TG-TT-GG-CC is a saline-alkaline sensitive The SNP molecular marker combination disclosed by the invention is used for detecting the saline-alkaline tolerance of the elymus dahuricus, is accurate and reliable, and effectively improves the identification efficiency of the germplasm and variety of the saline-alkaline tolerance elymus dahuricus.

The invention belongs to the technical field of fish breeding, and discloses a method for creating alkali-resistant grass carp new germplasm based on a ca12 gene editing technology and application. Carbonic anhydrase ca12 is used as a target gene, a CRISPR / Cas9 gene editing technology is used for carrying out gene editing on wild grass carp, the genotype of the grass carp is identified through sequencing, ca12 gene knockout F0-generation chimera and the corresponding wild type thereof are selected to carry out carbonate alkalinity stress experiments, and based on experimental results, it is known that the ca12 knockout chimera of the grass carp has high alkali resistance, and can be used for preparing the grass carp. The invention provides a novel breeding method and germplasm material for culturing fishes suitable for alkaline water culture, and has important industrial application value.

The invention belongs to the technical field of crop genetic breeding, and relates to a molecular marker linked with a peanut branch number related gene locus, an identification method and application of the molecular marker. The primer pair is C9-F / R, and the sequence of the primer pair is as shown in SEQ ID NO. 1 and SEQ ID NO. 2; by designing an InDel marker C9 linked with the number of branches, the character of the number of branches of peanuts can be quickly distinguished by using a primer to amplify a target band and using polyacrylamide gel electrophoresis detection. The identification method is more accurate and convenient, has good repeatability, can efficiently distinguish the phenotype and genotype of an individual, shortens the experiment process, has the advantages of simple and feasible operation steps and the like, and saves expenditure. The method disclosed by the invention has important application value in the breeding work aspects of peanut branch number character related gene positioning, genotype typing, later cultivation of excellent peanut varieties and the like.