2363 results about "Genotype" patented technology

The invention belongs to the technical field of genetic breeding, and particularly relates to a molecular marker influencing sheep weight traits and application thereof, and the molecular marker comprises a site I or a site II. The nucleotide sequence of the molecular marker containing the site I is as shown in SEQ ID NO.1, and mutation from T to C occurs at the 101bp position in the SEQ ID NO.1; the nucleotide sequence of the molecular marker containing the site 2 is as shown in SEQ ID NO.2, and mutation from A to G occurs at the 101bp position in the SEQ ID NO.2. According to the molecular markers influencing the sheep weight traits, the marker I and the marker II can be used for identifying the sheep weight traits, and the offspring weight can be increased by selecting TT or AA genotype individuals as male parents or female parents.

The invention discloses a sugarcane germplasm resource evaluation and breeding method for smart agriculture. The sugarcane germplasm resource evaluation and breeding method comprises the following steps: step 1, collecting materials with wide genetic diversity; establishing an intelligent incubator and a greenhouse, and monitoring environmental parameters by using an Internet of Things sensor; 2, performing data acquisition on the related germplasm resources by using an unmanned aerial vehicle, a robot and a near infrared spectrum technology, and constructing a phenotype database for data management; meanwhile, genotype identification is carried out by combining molecular marker-assisted selection and genome selection technologies, molecular markers of key genes are mined, and the genetic value of related germplasm is evaluated; step 3, constructing a hybrid combination prediction model by using an AI algorithm, optimizing parent matching of the related germplasm and the cultivated sugarcane, monitoring and collecting the filial generation at the same time, and analyzing and screening in combination with smart agriculture; 4, demonstration planting is conducted on the bred excellent sugarcane strain, and application of the excellent strain is improved through government-enterprise-scientific research institution collaborative popularization and in combination with farmer technical training.

The invention discloses a molecular marker for auxiliary screening of anti-freezing wheat germplasm and application of the molecular marker, and relates to the technical field of biology, in particular to a method for screening or auxiliary screening of wheat with different freezing resistance, which comprises the following steps: detecting whether the genotype of wheat to be detected is qFDG-7A. 3a or qFDG-7A. 3b, and the freezing resistance gt of the wheat with the genotype qFDG-7A. 3a; the freezing resistance of the wheat with the genotype qFDG-7A. 3b; the wheat of which the genotype is qFDG-7A. 3a is wheat of which the genotype is AA homozygous based on an A129C SNP (Single Nucleotide Polymorphism) site; the wheat of which the genotype is qFDG-7A. 3b is wheat of which the genotype is CC homozygous based on an A129C SNP (Single Nucleotide Polymorphism) site; the A129C SNP site is the 129th nucleotide from the 5'tail end of SEQ ID NO: 1 in a wheat genome. The molecular marker has important theoretical significance and economic value for molecular marker-assisted selection of wheat germplasm or breeding progeny materials with high anti-freezing capacity.

This disclosure provides a novel method and system for characterizing morphodynamic profiles of objects, such as biological entities. This disclosure provides a shape, appearance, and motion (SAM) phenotype Observation Tool (SPOT). SPOT establishes a standardized SAM “phenome,” image descriptors resembling single-cell transcriptomes, to comprehensively quantify a cell's instantaneous state without prior knowledge. SPOT also establishes a standardized workflow for temporal analysis. SPOT is a generalist tool, applicable to any live-cell imaging and advances biomedical discovery through its standardized, unbiased, streamlined workflow to quantify phenotypic heterogeneity and predict phenotype-genotype-function coupling.

The invention discloses an SNP molecular marker related to the conception rate of young cows, a detection product and application of the SNP molecular marker, and belongs to the technical field of dairy cow breeding markers. The SNP molecular marker is chr6: g. 103355581Cgt, and the SNP molecular marker is a molecular marker. The method comprises the following steps of: taking a bovine reference genome version UMD3.1 as a standard for a coordinate position, numbering Assessment in NCBI (National Center of Biotechnology Information) as GCF000003055.6, and numbering a chromosome as AC 000163.1; the young cows are cows which are 12-24 months old and do not lay calves. By detecting the genotype of the SNP site, the conception rate condition can be effectively judged, parent individuals with the higher young cow conception rate can be screened, early selection of genotype individuals with the high young cow conception rate is achieved, the frequency of the genotype with the high young cow conception rate in a dairy cow group is increased, and the reproduction rate of the dairy cow group is increased; the service life of cows is prolonged, the treatment cost is saved, the breeding cost is reduced, and the pasture income is increased.

The invention belongs to the technical field of aquatic biotechnology and molecular marker-assisted breeding, and particularly relates to a cynoglossus semilaevis vibrio harveyi-resistant KASP molecular marker combination and application thereof in cynoglossus semilaevis disease-resistant breeding. The KASP molecular marker combination comprises three SNP (Single Nucleotide Polymorphism) loci which are respectively positioned at 17314099, 17325496 and 17355989 loci of the 14 # chromosome of the cynoglossus semilaevis, when the genotypes of the three SNP loci are respectively CC, AA and GG, the disease resistance of the cynoglossus semilaevis is high, and the invention also provides a primer for detecting the genotype polymorphism of the SNP loci. By utilizing the KASP molecular marker combination, the vibrio harveyi resistance genotype in a cynoglossus semilaevis breeding population can be quickly detected, and disease-resistant individuals are screened, so that the disease-resistant breeding efficiency of the cynoglossus semilaevis is greatly improved, and a new variety of the cynoglossus semilaevis with strong disease resistance can be quickly cultivated.

The invention discloses a KASP molecular marker for identifying soybean hundred-grain weight and application thereof, and relates to the technical field of biology and application of an SNP locus, the SNP locus takes a soybean Wm82. A2. V1 genome sequence as a reference genome, the SNP is the 16321243th SNP on a soybean 11 # chromosome, and the SNP is the 16321243th SNP on the soybean 11 # chromosome. Corresponding to the 20th basic group from the 5'terminal of the sequence shown in SEQ ID NO: 1, when the locus is CC homozygosis, the corresponding genotype is A; when the locus is TT homozygous, the corresponding genotype is B; according to the application, soybeans with different hundred-grain weights are screened or screened in an auxiliary mode, and the different hundred-grain weights of the soybeans are as follows: the homozygous soybeans with the genotype A are higher than or candidate to be higher than the homozygous soybeans with the genotype B. The molecular marker has important theoretical significance and economic value for molecular marker-assisted selection of soybean germplasm or breeding progeny materials with high hundred-grain weight.

The invention discloses a production management system and method based on an intelligent production line, which are suitable for a tobacco process production line, and the method comprises the following steps: collecting genotype information and physicochemical indexes of tobacco raw materials; based on a small sample expansion algorithm and a neural network model of transfer learning, performing process matching analysis on the genotype information to generate an initial processing parameter set; in the cut tobacco making process, the Maillard reaction process is scanned and monitored in real time through a transmission-type THz-TDS probe array, the terahertz spectrum characteristic peak intensity is obtained, microwave auxiliary parameters are dynamically adjusted according to the characteristic peak, and a reaction optimization instruction set is generated; performing aroma constraint verification on the initial processing parameter set and the reaction optimization instruction set, and outputting a feasible process scheme; and executing the feasible process scheme, collecting finished product quality information, and updating the neural network model. According to the method, dynamic optimization and accurate regulation and control of the tobacco primary processing process are achieved, and the product quality stability and aroma type consistency are effectively improved.

The invention relates to the technical field of molecular biology, and particularly provides an InDel molecular marker related to the size of a lychee kernel and application of the InDel molecular marker. The InDel molecular marker is located at a site 25610680 of a fifth chromosome of a reference genome GeneBank: GCA019925255.1 of the litchi, and the InDel molecular marker is used for detecting the DNA of the litchi. The genotype of the marker in a pyrokaryotic litchi material is homozygous' GAAAAA / GAAAAA ', and the genotype of the marker in a macrokaryotic litchi material is heterozygous' G---- / GAAAAA 'or homozygous' G---- / G----'. The invention further provides a primer group for detecting the molecular marker, an effective tool is provided for molecular marker-assisted breeding of the size of the lychee kernel, and the method is expected to assist in shortening the breeding period, improving the breeding efficiency and promoting the improvement work of the lychee variety.

The invention provides a molecular marker related to immune traits of elephant male semi-fine wool sheep and a detection primer and application thereof, and belongs to the technical field of molecular assisted breeding. The nucleotide sequence of the molecular marker is shown as SEQ ID NO.1; the molecular marker comprises an SNP site, the SNP site is located at the 117th site of the molecular marker, the mutation basic group of the SNP site is A or G, and the immune globulin content of an individual male semi-fine wool sheep with the genotype of AG or GG at the SNP site is remarkably higher than that of an individual with the genotype of AA. The content of immune globulins IgA, IgG and IgM of an individual elephant male semi-fine-wool sheep can be judged according to the genotype of the molecular marker, the immune traits of the elephant male semi-fine-wool sheep can be rapidly and accurately evaluated, the high-immunity elephant male semi-fine-wool sheep can be screened, the molecular marker has important significance on early-stage auxiliary breeding of the elephant male semi-fine-wool sheep, the breeding period is remarkably shortened, and the breeding cost is reduced. The breeding efficiency is improved.

The invention belongs to the technical field of crop genetic breeding, and relates to a linked molecular marker of a peanut hypocotyl elongation related gene locus, an identification method and application of the linked molecular marker. The primer pair is C2-15-F / R, and the sequence of the primer pair is as shown in SEQ ID NO. 1 and SEQ ID NO. 2; by designing an InDel marker C2-15, the peanut hypocotyl length character can be quickly distinguished by using a primer to amplify a target band and using polyacrylamide gel electrophoresis detection. The identification method is more accurate and convenient, has good repeatability, can efficiently distinguish the phenotype and genotype of an individual, shortens the experiment process, has the advantages of simple and feasible operation steps and the like, and saves expenditure. The method disclosed by the invention has important application value in the breeding work aspects such as peanut hypocotyl elongation character related gene positioning, genotype typing and later cultivation of excellent peanut varieties.

The invention discloses SSR (simple sequence repeat) multiple PCR (polymerase chain reaction) primers for paternity test of grass carp and application of the SSR multiple PCR primers. The primers comprise 16 pairs of specific primers, and base sequences of the 16 pairs of specific primers are respectively shown as SEQ ID NO.1-32 in sequence. The invention further discloses a fish paternity test kit comprising the SSR multiple PCR primer, an SSR multiple fluorescent PCR method for grass carp paternity test and application of the SSR multiple PCR primer, the kit or the method in grass carp paternity test. According to the kit, 16 pairs of specific SSR primers, multiple fluorescent PCR and a universal amplification primer technology are combined, 16 sites can be detected at a time, universal primers can be repeatedly used, and compared with simple single-site detection, the efficiency is improved, and the cost is reduced; the microsatellite loci contained in the invention are 3-6 basic groups, the allele size interpretation is more accurate, and the accuracy of genotype data is improved.

The invention relates to the technical field of cold-chain logistics monitoring, in particular to a cold-chain logistics remote monitoring platform and method based on the Internet of Things. An environment monitoring unit collects position and temperature and humidity data through a sensor array; the goods decay map generation unit is combined with a genotype biological activity database and a cross-stage accumulative exposure analysis model, a real-time quality decay coefficient is output through a space-time stress coupling algorithm and biological verification, the block chain dynamic evidence storage unit generates a traceability block containing decay data and an environment hash value, and the traceability block is used for storing the quality decay coefficient. And a cross-stage association chain is constructed to form a life cycle quality map, the adaptive regulation and control unit dynamically generates a threshold value according to a decay coefficient and a goods value loss function, and triggers responsibility report pushing and emergency goods scheduling, and the scheme improves the accuracy of quality evaluation, makes responsibility division clear, and ensures efficient and stable operation of cold-chain logistics.

The invention relates to a molecular marker related to the content of 2-acetyl-3-methylpyrazine in pig muscle and application of the molecular marker, belonging to the technical field of determination or inspection methods of enzymes, nucleic acids or microorganisms. The technical problem to be solved by the invention is to provide a molecular marker related to the content of 2-acetyl-3-methylpyrazine in pig muscle. In order to solve the technical problem, the molecular marker provided by the invention is obtained through genome-wide association (GWAS) analysis, the nucleotide sequence of the molecular marker is as shown in SEQ ID NO: 1, and the molecular marker has an Agt at the 6328556 position of a chromosome 9 of a pig genome; a nucleotide single base of G is mutated, and the mutation obviously affects the content of 2-acetyl-3-methylpyrazine in pig muscles. By detecting the genotype of a to-be-detected pig at the SNP site, early selection of the content of 2-acetyl-3-methylpyrazine in pig muscles can be realized.

The invention relates to the technical field of Chinese trumpet creeper breeding, and discloses a Chinese trumpet creeper-based efficient breeding decision support system and method. The method comprises the following steps: acquiring multi-dimensional phenotypic data in a Chinese trumpet creeper breeding test, wherein the multi-dimensional phenotypic data comprises plant height, leaf area, flowering period and yield indexes; performing normalization processing on the multi-dimensional phenotypic data, eliminating influences of different dimensions, and calculating a variable coefficient of each phenotypic index; then screening out phenotypic indexes of which the stability is higher than a preset threshold value based on the variable coefficient, and constructing a Chinese trumpet creeper phenotypic feature matrix; carrying out dimensionality reduction on the Chinese trumpet creeper phenotypic feature matrix by adopting an adaptive weighting algorithm, and extracting key phenotypic features; inputting the key phenotypic features into a genetic algorithm optimization module, and calculating the contribution degree of each genetic locus in combination with the Chinese trumpet creeper genotype data; and constructing a Chinese trumpet creeper breeding decision model according to the contribution degree of the gene locus, and outputting an optimal breeding combination scheme. The system can optimize the Chinese trumpet creeper breeding process and provide effective support for Chinese trumpet creeper breeding work.

The invention belongs to the technical field of sweet potato breeding, and provides an SNP marker related to sweet potato root color and anthocyanin content and application of the SNP marker, the SNP marker is located at the 25658800th site of a chromosome 10a of sweet potatoes, and the base polymorphism is A / T. When the SNP genotype of the sample to be detected is A / T, the content of anthocyanin in the tuberous roots is high, and the color is purple; when the SNP genotype is T / T, the content of anthocyanin in the tuberous roots is low, and the tuberous roots are non-purple in a large probability. According to the detection method and the kit provided by the invention, the efficiency of identifying the purple sweet potatoes by the developed SNP marker through sweet potato natural population phenotype identification and verification reaches 100%, the efficiency of identifying the non-purple sweet potatoes reaches 85.04%, the color of the sweet potato tuberous roots and the anthocyanin content can be rapidly and accurately detected, and the detection method and the kit have the advantages of being simple and convenient to operate, low in cost and the like, and have wide application prospects. The requirement of large-scale molecular marker-assisted selection can be met.

The invention discloses development and application of a KASP marker related to wheat freezing resistance, and relates to the technical field of biology, in particular to a method for screening or assisting in screening wheat freezing-resistant germplasm, which comprises the following steps: detecting whether wheat to be detected is genotype qFR-1B. 1a or genotype qFR-1B. 1b, and detecting whether the wheat to be detected is genotype qFR-1B. 1a or qFR-1B. 1b; the freezing resistance of the genotype qFR-1B. 1a wheat is greater than or candidate greater than that of the genotype qFR-1B. 1b wheat; the wheat of which the genotype is qFR-1B. 1a is wheat of which the genotype of a G101A SNP (Single Nucleotide Polymorphism) site is a GG homozygous type; the wheat of which the genotype is qFR-1B. 1b is wheat of which the genotype of a G101A SNP (Single Nucleotide Polymorphism) site is AA homozygous; the G101A SNP site is the 101 nucleotide from the 5'tail end of SEQ ID NO: 1 in a wheat genome. The molecular marker has important theoretical significance and economic value for molecular marker-assisted selection of wheat germplasm or breeding progeny materials with relatively high freezing resistance.

The invention relates to the technical field of biological breeding, in particular to a biological breeding management method and system based on big data. The method comprises the following steps: acquiring original germplasm data; multi-source heterogeneous data integration is carried out on the original germplasm data, the data integration comprises genotype sequencing integration and historical phenotype integration, and integrated germplasm data is generated and stored in a database in a distributed mode; obtaining original growth data, performing data preprocessing, generating standardized germplasm resource comprehensive data including a crop image feature set and an environment response parameter set, and synchronizing the data to the database; and performing whole genome selection prediction on the integrated germplasm data based on the standardized germplasm resource comprehensive data to generate a genotype-phenotype prediction model. By integrating germplasm resources, field management, breeding management, phenotype management, genotype management and whole genome selection, the accuracy and adjustment flexibility of biological breeding management are improved.

The invention provides a molecular marker related to immune traits of elephant male semi-fine wool sheep and application of the molecular marker, and belongs to the technical field of molecular marker application, and the nucleotide sequence of the molecular marker is shown as SEQ ID NO.1; the molecular marker comprises an SNP site, the SNP site is located at the 363rd site of the molecular marker, the basic group of the SNP site is T or C, and the immune globulin content of a male semi-fine wool sheep individual with the genotype of TC or CC at the SNP site is remarkably higher than that of a male semi-fine wool sheep individual with the genotype of TT. By detecting the genotype of the molecular marker SNP site related to the immune traits in the male semi-fine-wool sheep, the immune traits of the male semi-fine-wool sheep can be rapidly and accurately evaluated, and the molecular marker SNP site has important significance on early-stage auxiliary breeding of the male semi-fine-wool sheep.

The present invention relates to a method for constructing an eight-gene-edited xenogeneic organ transplantation donor pig, belonging to the field of animal biotechnology. In the wild-type porcine fetal fibroblast cell line, the GGTA1, β4GalNT2, and CMAH genes are knocked out by using the CRISPR / Cas9 gene editing technology, and the humanized genes of hCD39, hCD46, hCD55, hCD59, and hTBM are transfected. Combining with somatic cell cloning technology, GTKO / β4GalNT2KO / CMAHKO / hCD39 / hCD46 / hCD55 / hCD59 / hTBM eight-gene-edited cloned pigs are constructed. Further, through genotype, mRNA, protein expression identification and functional analysis, eight-gene-edited xenogeneic organ transplantation donor pigs are obtained. The present invention solves the technical problems of high production difficulty, low efficiency, and low survival rate of donor pigs for multi-gene-edited xenogeneic organ transplantation, and maximally solves the common problems of immune rejection reaction and complement dysregulation faced during xenogeneic organ transplantation, laying a foundation for more targeted development of donor pigs suitable for different tissue and organ xenotransplantation, and having important value for promoting the clinical transformation of xenogeneic organ transplantation.

The invention belongs to the technical field of genetic breeding, and particularly relates to application of a molecular marker for breast circumference character breeding of grassland short-tailed sheep, the molecular marker is at least one of the following (1)-(3): (1) a nucleotide sequence as shown in SEQ ID NO.1, nucleotide at the 101bp site is A or G; (2) a nucleotide sequence as shown in SEQ ID NO.3, wherein the nucleotide at the 101bp site is C or A; (3) a nucleotide sequence as shown in SEQ ID NO.5, wherein the nucleotide at the 101bp site is T or C; the application refers to any one of the following steps: identifying the chest circumference of the grassland short-tailed sheep; the chest circumference of the offspring of the grassland short-tailed sheep is improved. By detecting the genotype of the molecular marker and selecting a dominant genotype individual as a parent, the average chest circumference of offspring can be effectively improved, and a reliable tool is provided for realizing molecular assisted breeding and precise genetic improvement of the chest circumference character of the grassland short-tailed sheep.

The invention relates to the technical field of molecular markers, in particular to a biomarker related to the zeaxanthin content character of sweet corn kernels and application of the biomarker. The RIL population is used for performing QTL positioning on the corn kernel zeaxanthin content character, it is found that a major QTL related to the sweet corn kernel zeaxanthin content exists on the sixth chromosome of corn, the major QTL is located between SNP sites Schr6: 83027949 and Schr6: 84835891, the phenotype contribution rate of the corn kernel zeaxanthin content is 18.69%, and the major QTL is a major QTL related to the sweet corn kernel zeaxanthin content. The major QTL can be used for screening or auxiliary screening of sweet corn with relatively high zeaxanthin content, when the genotype of Schr6: 83027949 is GG and the genotype of Schr6: 84835891 is AA, the zeaxanthin content is relatively high, technical support is provided for molecular marker-assisted breeding, and the major QTL has important significance in improving the nutritional quality of the sweet corn.

A crop genotype-to-phenotype general prediction method based on multi-task learning comprises the following steps: collecting genotype and phenotype data sets of five crops, performing quality control and coding on genotype data, and performing standardization processing on phenotype data; dividing the data set into a training set and a test set in proportion by adopting a stratified sampling method; by taking genotype data as input and phenotype data as output, a universal prediction model based on a multi-task learning framework is constructed, information sharing among different phenotype prediction tasks is realized, and potential relations between complex phenotypes and genotypes are accurately captured. According to the method, phenotypes can be accurately predicted according to genotypes in the early stage of breeding, so that materials with target characters are rapidly screened, the breeding period is effectively shortened, the breeding efficiency is remarkably improved, and an efficient and universal technical scheme is provided for crop breeding and related research.

The invention discloses a molecular marker closely linked with a cowpea salt tolerance major QTL (quantitative trait loci) and a primer sequence of the molecular marker. The name of the cowpea salt tolerance major QTL is SC300124, a KASP type molecular marker 300124 closely linked with the cowpea salt tolerance major QTL is located at the position of 43716091 bp on a chromosome 11, the marker can be used for amplifying a material to be detected, and whether a sample carries key allelic variation for controlling salt tolerance or not is judged according to the genotype of the marker. The molecular marker and the primer provided by the invention can be used for quickly screening the cowpea salt tolerance major QTL, and compared with a traditional phenotype identification method, the molecular marker and the primer have the advantages of high detection flux, accurate and reliable result and the like, and the typing accuracy can reach 99% or above. The technology can significantly improve the breeding efficiency of the salt-tolerant variety of cowpea and shorten the breeding period.

The invention discloses a molecular marker related to thousand seed weight of wheat, a detection primer and application of the molecular marker and the detection primer, and belongs to the technical field of molecular marker-assisted breeding. The nucleotide sequence of the molecular marker TaDTX50-STS is as shown in SEQ ID NO. 1; basic groups from the 502 site to the 661 site of the sequence as shown in SEQ ID NO. 1 have insertion / deletion mutation. The InDel molecular marker related to the thousand seed weight character in a wheat genome is identified and named as TaDTX50-STS, and by detecting the polymorphism or genotype of the InDel molecular marker, the InDel molecular marker can be used for identifying or assisting in identifying the thousand seed weight character of wheat, assisting in breeding dominant varieties with high thousand seed weight of wheat and improving the wheat breeding efficiency.

The invention relates to the technical field of agricultural biology, in particular to SNP related to soybean node spacing and development and application of a KASP primer of the SNP, S121140167 remarkably related to the soybean node spacing is located at the 12th chromosome 1140167 bp position of a soybean genome Wm82. A4. V1, the site polymorphism is C or T, and the TT genotype is used for providing technical support for molecular marker-assisted breeding of the soybean node spacing character. The invention further develops a KASP molecular marker which can directly perform specific distinguishing and detection on the C or T basic group of the SNP mutation site, and the marker can quickly and accurately identify the soybean internode spacing, reduce the influence of the environment on the identification of the soybean internode spacing character, shorten the breeding cycle and accelerate the selection and improvement of the soybean internode spacing character.

The invention relates to the field of molecular markers, in particular to application of an SNP (Single Nucleotide Polymorphism) molecular marker in identification or auxiliary identification of goat chest circumference. The invention provides an SNP (Single Nucleotide Polymorphism) molecular marker related to a goat chest circumference character. The SNP molecular marker comprises SNP01-SNP06; wherein the SNP01, the SNP02 and the SNP04 are all beneficial mutations, and the remarkable dominant genotypes of the SNP01, the SNP02 and the SNP04 are CC, GG and CC respectively; sNP03 and SNP05 are both harmful mutations, and the obvious dominant genotypes are TT and AA respectively; sNP06 is beneficial mutation, and the significant dominant genotype is GG. The SNP molecular marker provided by the invention is related to the chest circumference character of the goat, and the chest circumference character of the goat can be more accurately predicted, so that effective selection is carried out in the breeding process, the breeding efficiency of the goat with larger chest circumference is improved, and a new molecular marker resource is also provided for genetic breeding of other livestock.

The invention relates to the technical field of modern livestock breeding, in particular to application of an MC5R gene in detection of goat growth traits. The invention provides application of an MC5R gene in detection of goat growth traits, a molecular marker related to the goat growth traits exists at the 519bp position of the nucleotide sequence of the MC5R gene, and the polymorphism of the molecular marker is C / T. When the genotype of the polymorphic site contained in the molecular marker is CC, the genotype corresponds to the growth trait relative advantage of the goat to be detected; when the genotype of the polymorphic site contained in the molecular marker is TT, the growth traits of the goat to be detected are relatively inferior. The molecular marker provided by the invention can be applied to identification or prediction of the growth state of the goats, can also be applied to the field of molecular breeding, and judges the genetic potential, seed reservation and seed reproduction of the goats on the basis of analysis of the genotypes of the goats. The invention has great significance for modern livestock breeding of goats.

The invention relates to the technical field of molecular markers, in particular to an Indel molecular marker related to yellow leaves of a melon seedling growing point and application of the Indel molecular marker. The invention explores a key candidate gene MELO3C016136 for controlling the leaf color of the growth point of the muskmelon seedling, and develops an Indel molecular marker ygp2 for identifying the leaf color change of the growth point of the muskmelon seedling based on the key candidate gene MELO3C016136. The molecular marker ygp2 can be used for identifying the leaf color change character of the growth point of the muskmelon seedling, only one-time PCR amplification and polyacrylamide gel electrophoresis need to be carried out on the DNA of the plant to be detected, the identification result is reliable, the genotype of the target plant can be simply and conveniently identified, and the genotype identification result is completely consistent with the leaf color phenotype of the growth point of the seedling; important technical support is provided for creating new muskmelon germplasm with yellow seedling growing point leaves.

The invention relates to application of a molecular marker related to daily gain of pigs at the stage of 30kg-120kg. The invention discloses a method for identifying the daily gain of pigs at the stage of 30 kg to 120 kg and a breeding method for breeding the pigs with the daily gain at the stage of 30 kg to 120 kg. According to the method, by detecting the genotype of a to-be-detected pig at the 161610871th site of a chromosome 1 in a pig reference genome Susscrofa.Sscrofa11.1, the daily gain of the pig at the stage of 30 kg to 120 kg is identified, the pig with the TT genotype is higher than the pig with the CT genotype, and the pig with the CT genotype is higher than the pig with the CC genotype. In actual breeding work, a to-be-detected pig with the genotype of the C161610871T site being TT can be selected as a parent for breeding, and the method has important significance on selection of pigs with high feed intake and high feed utilization efficiency.