38 results about "Mammalian Embryos" patented technology

An improved method of nuclear transfer involving the transplantation of donor differentiated cell nuclei into enucleated oocytes of the same species as the donor cell is provided. The resultant nuclear transfer units are useful for multiplication of genotypes and transgenic genotypes by the production of fetuses and offspring, and for production of isogenic CICM cells, including human isogenic embryonic or stem cells. Production of genetically engineered or transgenic mammalian embryos, fetuses and offspring is facilitated by the present method since the differentiated cell source of the donor nuclei can be genetically modified and clonally propagated.

A method of generating cells capable of secreting insulin is disclosed. The method comprises subjecting mammalian embryonic stem cells to set of culturing conditions suitable for differentiation of at least a portion thereof into cells displaying at least one characteristic associated with a pancreatic islet cell progenitor phenotype, and subjecting such differentiated cells to a set of culturing conditions suitable for formation of surface bound cell clusters including insulin producing cells.

The invention relates to isolated hemangioblast cells. Hematopoietic and endothelial cells are postulated to be derived from a common progenitor, hemangioblast. While hemangioblast has been isolated retrospectively during embryonic stem cell differentiation, it has not been isolated from embryos or from bone marrow. Prospectively stable clonal cell lines have been isolated from mammalian embryos, from embryonic stem cells and from mammalian bone marrow that can differentiate in vitro into tubular structures with both endothelial and hematopoietic markers such as CD34, CD31, Flk-1, TIE2, P-selectin, Sca-1, thy-1, CD45, and smooth muscle actin. Gene expression profiles in the undifferentiated and differentiated cells were consistent with endothelial and hematopoietic differentiation potential. Transplantation studies in isogenic or immunodeficient mice demonstrated that these cells were not tumorigenic. In an appropriate microenvironment, the cells incorporate into the vasculature and participate in hematopoiesis.

The present invention relates to a method for generating non-human mammalian chimeric embryo. The method involves coculturing denuded non-human mammalian embryos with cells in an Eppendorf micro test tube. The chimeric embryo obtained is then transferred into a non-human recipient mammal so as to develop into a non-human chimeric fetus, non-human chimeric mammal, an embryonic stem cell-derived fetus or an embryonic stem cell-derived mammal.

Mechanisms regulating cell proliferation stop and differentiation initiation during the development stage of mammalian embryo, and the proteins involved therein, are presented. Differentiation regulators, methods of regulating differentiation, transgenic organisms with loss of expression of the differentiation regulator, and methods of preparing the transgenic organisms, are provided.

Microfluidic embryo scaled channels (14) for handling and positioning embryos provide the opportunity to evaluate and treat embryos in improved manners. Fluid flow is used to move and position embryos within microfluidic channels and channel geometrics may be used to place embryos at specific locations. Surface properties and compliance (deformation) properties of embryos are evaluated as a predictor of viability. The microfluidic channels provide the opportunity for fine controls of pressure to conduct various evalutions at forces slightly below which damage to embryos is known to occur. Measurement of the distance and/or which embryos roll in a same pressure gradient microfluidic channel provides information, with healthy embryos traveling slower or a shorter distance as they demonstrate more stiction to channel walls. Positioned at a constriction (14a, 14b, 24, 26), health embryos also appear to deform less than unhealthy embryos that are more readily pulled into a constriction. In addition, healthy embryos appear to resume their shape better. Fluid from microfluidic channels is easily collected downstream without altering the embryo environment, providing a better opportunity for chemical analysis of fluid chemical analysis than convention manual handling and sampling techniques. Zona pellucida removal of mammalian embryos is achieved as embryos are moved through flow to a precise location where lysing agent can be washed over the embryo to achieve zona removal. Cumulus removal is realized with a series of constrictions to cut cumulus followed by fluid flows to remove cut cumulus from the embryo.

The invention provides methods and systems for assessing the developmental potential of mammalian embryos. The method of the invention comprises taking measurements of cytoplasmic movements in the embryo and/or periodic changes in the shape of the embryo at the single cell stage.

The invention discloses an in vitro micro-dynamic culture device of mammalian embryos and a method for cultivating embryos by using the same, relates to an in vitro culture device of embryos and a cultivation method using the same, and overcomes the defects of an in vitro oscillating micro-dynamic incubator of mammalian embryos and the defects of an existing patent technology. The culture device comprises a glass micro-fluid channel layer, a main body layer, an air chamber seal cover, an air duct, an injector, an electric motor and a square wave generator. The cultivation method comprises the following steps: 1, setting the frequency of the square wave generator and the stretching speed of the electric motor; 2, injecting a culture solution and mineral oil, and putting the embryos into a culture room; and 3, powering on a switch, and cultivating. The in vitro micro-dynamic culture device is applied to the field of biology.

The present invention relates to complement proteins, in particular, C3 protein, and methods using the same for enhancing the development of preimplantation mammalian embryos in vitro for use in assisted reproductive technologies. In particular, the present invention relates to supplementation of complement C3 protein, its precursors, fragments, or derivatives, to culture media to improve the development of cultured embryos and, thereby, enhance pregnancy rates of in vitro fertilization.

The invention relates to a method for preparing and separating stereotyped endoderm cells by using monolayer culture technology and also discloses culture mediums suitable for preparing the stereotyped endoder mcells. The culture mediums comprise Neurobasal culture medium, DMEM/F12 culture medium, N-2 additive, B-27 additive, bovine serum albumin, mono-thioglycerin and activin A. The inventive method is simple and efficient, and can improve the efficiency in differentiating stereotyped endoderm of embryonic stem cells (preferably inhuman mammals) greatly.

A method of enhancing in vitro development of a mammalian embryo is disclosed which comprises supplementing the culture medium with a prostaglandin, or a prostaglandin analog, in an amount effective to promote complete hatching of the embryo (i.e., freeing of the embryo from the zona pellucida). The quality of human blastocysts is enhanced in vitro by culturing with a prostacyclin agonist, Iloprost. The in vivo implantation potential and live birth potential of an in vitro fertilization embryo is thereby enhanced and establishment of a viable pregnancy is facilitated.

The invention relates to a PCR augmentation primer and agent box and the application in the sex identification of mammals, which is a pair of primer augmenting the 163bp area in gene Sry, sequence of basic group is: SRY5: 5'-TGAACGCCTTCATTGTGTGGTC-3',SRY3: 5'-GCTAGTAGTCTCTGTGCCTCCT-3'. The main components of the agent box are: buffer for DNA extraction, Taq DNA polymerase, PCR reaction liquid, blank control, paraffin oil and sample buffer. The applying steps of agent box in the sex identification of mammals are: (1) sample treatment, (2) augmentation, (3) product detecting. The invention is characterized by judging the existence of Y colorant layer specific fragment by detecting the augmenting product, and the avoidance of the unnecessary pollution, and the suitness for the quick(about 1 hour) sex identifying of mammal embryo in practice, the accuracy is 100%.

The invention relates to isolated hemangioblast cells. Hematopoietic and endothelial cells are postulated to be derived from a common progenitor, hemangioblast. While hemangioblast has been isolated retrospectively during embryonic stem cell differentiation, it has not been isolated from embryos or from bone marrow. Prospectively stable clonal cell lines have been isolated from mammalian embryos, from embryonic stem cells and from mammalian bone marrow that can differentiate in vitro into tubular structures with both endothelial and hematopoietic markers such as CD34, CD31, Flk-1, TIE2, P-selectin, Sca-1, thy-1, CD45, and smooth muscle actin. Gene expression profiles in the undifferentiated and differentiated cells were consistent with endothelial and hematopoietic differentiation potential. Transplantation studies in isogenic or immunodeficient mice demonstrated that these cells were not tumorigenic. In an appropriate microenvironment, the cells incorporate into the vasculature and participate in hematopoiesis.

The invention relates to a sex identification method for a mammalian embryo. The sex identification method for the mammal embryo comprises the following steps: (1) obtaining the embryo; (2) obtaining genome DNA of embryonic cells; (3) designing and synthesizing primers and probes; (4) performing QPCR reaction: during PCR amplification, adding a pair of primers and a specific fluorescent probe at the same time; and (5) amplifying the curve and analyzing, and identifying the sex of the embryo. The sex identification method for the mammal embryo, disclosed by the invention, is a real-time fluorescent quantitative PCR (Quantitative Real-time PCR) probe method, is simple in design, only needs a pair of primers and is added with a specific fluorescent probe at the same time, so that the limitation that only end-point detection can be carried out in a traditional method is effectively solved; the method has the advantages of less sampling, high sensitivity, stronger specificity, rapidness, simplicity, convenience, effective solving of a PCR pollution problem, good universality, high automation degree and the like.

The invention relates to a method for identifying the sex of a mammal embryo. The method for identifying the sex of the mammal embryo comprises the following steps: (1) obtaining the embryo; (2) obtaining genome DNA of the embryonic cells; (3) designing and synthesizing primers; (4) performing QPCR reaction: adding a fluorescent dye into a PCR reaction system; and (5) amplifying the curve and analyzing, and identifying the sex of the embryo. The method for identifying the sex of the mammal embryo, disclosed by the invention, adopts the real-time fluorescent quantitative PCR method, is simple in design, only needs a pair of primers, can analyze and detect the specificity of amplification reaction through a melting point curve, and has the advantages of high sensitivity, short time consumption, low initial cost, good universality, high automation degree and the like.

Described herein is a method for treating or restoring loss of hearing and balance in a subject in need thereof. In certain aspects, the disclosed method is comprised of transplanting a humanized otocyst to a target delivery site in the subject. According to certain aspects, the otocyst is obtained from a humanized non-human mammal embryo. In certain exemplary aspects, the non-human mammal is a pig. In certain embodiments, prior to transplantation, the otocyst is cultured in a species-specific tissue culture buffer.

Methods and compositions for enhancing development of a preimplantation mammalian embryo and for increasing the live birth potential of an in vitro fertilized mammalian embryo are disclosed. An in vitro method of activating the peroxisome proliferator activated receptor δ (PPARδ) in a preimplantation mammalian embryo comprises culturing an embryo in an embryo culture medium, and upon or after commencement of expression of PPARδ in the cells of the embryo, activating the PPARδ by adding an amount of a PPARδ ligand to said medium effective to bind to PPARδ to deter apoptosis in the cells of the cultured embryo and/or increase proliferation of the cells of the cultured embryo.