250 results about "Prenatal development" patented technology

The invention relates to the technical field of database construction, in particular to a construction method and system for a reproductive medicine database. The method comprises the following steps: acquiring a biological tissue sample of a patient; acquiring reproductive endocrine hormone data, embryo morphological parameter data, immune cell function data and genome variation data from the biological tissue sample of the patient; performing time sequence analysis processing on the hormone fluctuation mode according to the reproductive endocrine hormone data to obtain endocrine dynamic characteristic data; performing image recognition processing based on coherent superposition characteristics of light on the embryo morphological parameter data, and performing embryo development potential evaluation to obtain embryo development potential data; and performing hierarchical clustering processing on the individual heterogeneity of the patient according to the immune cell function data and the genome variation data to obtain immune-genetic typing data of the patient. By means of the quantum dot sensing technology and the multi-channel micro-fluidic chip, standardization of data acquisition is achieved, and the heterogeneity problem of a data source is solved.

The invention belongs to the technical field of light polarization analysis, and discloses a method for determining the sex of an embryo in an egg based on polarization image recognition. Comprising the following steps: acquiring a transmitted spectrum image sequence of a hatching egg to be detected at a plurality of polarization angles, wherein the transmitted spectrum image sequence covers a transmitted spectrum image from visible light to a near-infrared band; extracting a polarization sensitive wave band set of the embryonic tissue according to the polarization state change characteristics of the embryo area in the transmitted spectrum image at different polarization angles; constructing an embryo gender characteristic spectrum based on the polarization degree distribution difference and the spatial texture characteristics of each wave band in the polarization sensitive wave band set; determining an optimal wave band combination for sex determination by analyzing the relevance between a polarization anisotropy index of a specific wave band in the embryo sex characteristic spectrum and an embryo development stage; according to the polarization response mode corresponding to the optimal wave band combination, the sex of the embryo in the egg is judged, and the resource investment and the waiting cost are greatly reduced.

The invention discloses application of MY-1B in preparation of medicines for improving ovarian aging, belongs to the technical field of biological medicines, and aims to solve the problems of how to effectively delay ovarian aging and improve ovarian functions. Experiments prove that the mRNA m5C modification level of ovarian granular cells in the ovarian aging process is increased, and the expression level of methylated transferase Nsun2 is increased. Ovarian aging caused by mRNA m5C modification level increase can be improved by supplementing the MY-1B, which is embodied in that the MY-1B can reduce the mRNA m5C modification level of aging nest granular cells; the MY-1B can be used for effectively correcting the orbital cycle disorder of the aged ovary and delaying the decline of the ovarian function; the MY-1B plays an important role in reducing the apoptosis level of granulosa cells in senescent ovaries; the MY-1B can improve the storage capacity of ovaries and enhance the quality of oocytes, so that the fertilization rate and the embryonic development potential of the oocytes are improved.

The invention provides application of a sperm tRNA (transfer ribonucleic acid) derived fragment 5 'tRF-Glu-CTC in prediction of development potential of assisted reproductive embryos. The expression of a tRNA derived fragment 5 'tRF-Glu-CTC in a sperm sample is detected, the abnormal expression of the sperm specific tRNA derived fragment 5' tRF-Glu-CTC in a DFI increase patient is verified, and the analysis on the correlation with clinical data and reproductive outcome of the patient prompts that the 5 'tRF-Glu-CTC molecular marker has significant correlation with the reproductive outcome, so that the 5' tRF-Glu-CTC molecular marker can be used for detecting the expression of the sperm specific tRNA derived fragment 5 'tRF-Glu-CTC in the DFI increase patient. The embryonic development potential can be effectively predicted, and a biomarker is provided for assisted reproduction treatment. In different experimental groups, the 5 'tRF-Glu-CTC can significantly improve the prediction precision, and shows good prediction performance in different clinical data. The detection method based on the fragment is expected to become a new tool for evaluating the assisted reproduction curative effect, the reproduction treatment scheme is further optimized, and the success rate is improved.

The invention provides an SMMC network-based embryo development quality evaluation method. The method comprises the following steps: inputting embryo images in different development periods; distributing different weights to the embryo image according to the development period, and outputting an attention feature map with channel attention; extracting global features and local features of the attention feature map; fusing the global features and the local features and extracting time sequence features to obtain a spatial-temporal feature map; and inputting the spatial-temporal feature map into a dichotomy full-connection layer for classification, and evaluating the embryonic development quality. By extracting the features in various aspects, the method has more excellent performance in embryo evaluation.

The invention relates to an N2B27 culture medium for inducing generation of bidirectional pluripotent stem cells, one of LY2090314, AS1842856 or CHIR99021 is added into an N2B27 basal culture medium, and the concentration of the CHIR99021 is 10 mu M. The invention also relates to a preparation method of the N2B27 culture medium. The weight of the LY2090314 is 10 nM, and the weight of the AS1842856 is 0.6 [mu] M. The invention also provides an application of the culture medium in induced production of bidirectional pluripotent stem cells. According to the invention, a novel stem cell culture condition for promoting co-expression of genes OCT4 and CDX2 by adding small molecules LY2090314 (LY), AS1842856 (AS) and CHIR99021 to inhibit signal channels of FOXO1 and GSK3 is screened out. The culture conditions are simple and convenient, the application range is wide, the cultured cells keep the characteristics of 16-32 cell stages of the embryos, and the development characteristics of the embryos can be efficiently reproduced. Under the condition of not depending on transcription factors, the screened bidirectional pluripotent stem cells (BPSCs) can promote efficient generation of TSC cells and establish a TSC cell line through independent differentiation of a serum culture medium or induced differentiation of a TSC culture medium. The obstacles between early embryo pedigree are broken, and the research on early embryo development is promoted.

The invention relates to a method for evaluating toxicity of spiroxamine by using zebra fish and application of the method, and belongs to the technical field of toxicity detection. According to the method, spiroxamine culture solutions with different concentrations are prepared for culturing zebrafish embryos, embryonic development detection and juvenile fish behavior testing are carried out, embryonic development detection includes hatching rate, heart rate and body length, and juvenile fish behavior testing includes spontaneous movement testing, vortex escape testing, swimming behavior testing, reflex behavior testing and color stimulation testing. According to the method provided by the invention, the toxic effect of spiroxamine on zebra fish embryos and juvenile fishes is systematically and comprehensively evaluated, the intuition and accuracy of spiroxamine toxicity detection are improved, and the method can be applied to evaluating the toxicity of environmental samples containing spiroxamine.

The invention discloses a dairy cow timed insemination method for controlling ovulation time and improving embryo implantation rate, and relates to the technical field of dairy cow breeding. The problems that follicles on ovaries of postpartum cows are not synchronous in development, ovulation time cannot be accurately regulated and controlled, and embryonic development regulation and control after fertilization are lacked are solved. The method mainly comprises the following steps: three times of gonadotropin releasing hormone injection, two times of cloprostenol sodium injection, gonadotropin releasing hormone injection, luteinizing hormone releasing hormone A3 injection, timed artificial semen deposition, and VADE mixed liquid injection. GnRH is used for actively regulating follicular development, 95% or more of the dairy cows are synchronized after 2-3 rounds of starting, then timing insemination is performed, the conception rate of the postpartum dairy cows can be remarkably increased, compared with the prior art, the pretreatment time is short, the synchronous ovulation rate and conception rate of the dairy cows can be increased, the calving interval is shortened, and the survival rate of the dairy cows is increased. The economic benefit of the pasture is obviously increased, and the breeding management level can be improved on the basis.

The invention relates to the technical field of biomedicine detection reagent manufacturing and reproductive medicine, in particular to an embryonic development potential sperm key protein kit and a use method thereof. The method is characterized in that an antibody combination capable of specifically recognizing a group of key proteins related to embryonic development potential in human sperms is prepared, and the antibodies, a fluorescence labeled secondary antibody, a cell nucleus dye, a concentrated washing liquid, a mounting medium and a pre-coated control porous glass slide are assembled into the special detection kit. The kit can be combined with a multi-mode imaging system (such as a fluorescence / confocal microscope and a common optical microscope) and image analysis software to realize the traditional analysis of sperm morphology and movement ability, and can perform synchronous and accurate qualitative and quantitative analysis with the expression level and subcellular localization of the key protein. The invention provides an integrated molecular detection solution, and provides a powerful tool for sperm quality screening and embryonic development potential prediction in an assisted reproduction technology.

The invention relates to a method for inducing mouse trophoblast stem cells in vitro. According to the method, mouse embryonic stem cells are used as starting cells, Sorcs3 gene knockout mouse embryonic stem cells are obtained through a gene editing technology, and in-vitro induced trophoblast stem cells (iTSCs) are efficiently and rapidly obtained by means of a flow cytometry sorting technology and in combination with specific antibody sorting. According to the invention, the Sorcs3 gene is knocked out from the embryonic stem cell, so that the capability of the embryonic stem cell to differentiate to the trophoblast lineage can be obviously enhanced; an important research platform is provided for deeply researching a lineage intertransformation mechanism of cell masses (ICM) and trophoblast (TE) in the blastocysts, and the method has important significance for researching lineage limitation of early embryonic development of mammals and the like.

A system and method for analyzing mechanical properties of biological tissues combines light-sheet fluorescence microscopy with mechanical perturbation capabilities. The system integrates a MEMS-based compression device, high-speed light-sheet scanner, and digital image correlation analysis to characterize viscoelastic properties of three-dimensional cellular structures. The system enables quantification of spatially-resolved mechanical characteristics and cellular responses through volumetric strain analysis, correlating mechanical properties with fluorescence-based cellular markers. Implementation with maternal-fetal interface models demonstrates capabilities in analyzing tissue mechanics during cellular invasion processes. The system provides applications in studying mechanical interactions in biological processes including embryonic development, cancer metastasis, and wound healing. The disclosed methodology enables unprecedented analysis of mechanical property dynamics at cellular interfaces in three-dimensional tissue environments.

The invention discloses an embryo development stage hierarchical classification method based on a convolutional neural network. The method comprises the following steps: acquiring an embryo image; acquiring a feature map according to the embryo image; fusing the feature maps and carrying out coarseness classification to obtain a main stage probability; fusing the feature maps after coarse granularity classification, and performing fine granularity classification to obtain a sub-stage probability; obtaining a category probability based on the main stage probability and the sub-stage probability; and performing hierarchical classification on the embryonic development stages according to the category probability. The method can improve the classification precision, and reduces the possibility of misclassification.

The invention discloses a bovine oocyte in-vitro maturation culture solution as well as a preparation method and application thereof, and belongs to the technical field of animal reproduction and embryo engineering. The preparation method of the bovine oocyte in-vitro maturation culture solution comprises the step of adding mitoquinine mesylate into a conventional IVM culture solution, wherein the adding concentration of the mitoquinine mesylate is preferably 50 nmol / L. The bovine oocyte in-vitro maturation culture solution provided by the invention can effectively reduce the oxidative stress reaction of the oocytes in the in-vitro culture process, enhance the mitochondrial function and remarkably improve the bovine oocyte in-vitro maturation quality and the embryonic development potential, so that the blastocyst rate is increased, and the problem that the development potential of the existing oocyte IVM culture solution is insufficient is solved.

The presently disclosed subject matter relates to using a haploid inducing line (whether existing or created) and transforming the haploid line so that it encodes cellular machinery capable of editing genes. The transformed haploid inducing line is used as a parent in a cross between two plants. During pollination, the parental gametes fuse to form an embryo; and the gene editing machinery is also delivered to the embryo at this time. During embryonic development, one set of parental chromosomes are lost, and the gene editing machinery operates on the remaining set of chromosomes. Thus, at least one haploid progeny with edited genes is produced from the cross.

This invention relates to the interdisciplinary field of assisted reproductive technology and artificial intelligence, specifically to a system and method for constructing an embryonic developmental trajectory map that integrates morphological features. By deeply integrating static morphological features with dynamic morphodynamic parameters, a more comprehensive panoramic map of embryonic development with richer information dimensions is constructed, overcoming the one-sidedness of single-dimensional assessment. Automated analysis of the map using an AI model reduces the influence of subjective human factors, enabling the discovery of subtle developmental patterns and potential differences that are difficult to discern with the human eye, significantly improving the accuracy of selecting high-quality embryos. The provided developmental trajectory map allows embryologists to intuitively examine the entire developmental process of the embryo and the performance of each key node, enhancing the interpretability and credibility of the assessment process. Through more scientific embryo selection, it is expected to improve embryo implantation and clinical pregnancy rates, and reduce multiple pregnancy and miscarriage rates.

Provided are a reagent combination or a kit for constructing an embryoid and the use thereof. The reagent combination or the kit can be used for inducing a stem cell to produce a blastocyst-like lineage precursor cell, a blastocyst-like cell and / or an embryoid, which are based on small-molecule induction and independent of transgenes, and have a single cell source. The produced blastocyst-like lineage precursor cell and blastocyst-like cell have a full blastocyst lineage, and are seed cells having balanced developmental competence and totipotency, which solves the problem of unbalanced cell maturation in the prior art. The produced embryoid is highly similar to natural embryos in terms of morphological characteristics and transcriptomic characteristics, has the capability of highly reproducing post-implantation embryonic development and / or gastrulation, which is beneficial for research on embryonic development in vitro. The method for inducing the stem cell to produce the blastocyst-like lineage precursor cell, the blastocyst-like cell and / or the embryoid by using the reagent combination or the kit is simple, and has high applicability and high embryoid construction efficiency.

The invention relates to the technical field of embryo quality evaluation, and discloses an embryo development quality evaluation method based on image recognition. The method comprises the following steps: acquiring an embryonic development image sequence, and judging whether the data volume reaches a preset acquisition threshold value; if a threshold value is reached, constructing an embryo feature vector, and processing the embryo feature vector by a multi-dimensional feature dynamic encoder to obtain optimized feature data; a dynamic game-reinforcement learning framework is adopted, and a development quality evaluation strategy is optimized through interaction of an evaluation agent and an embryo population agent; data collaboration is realized by means of a federated learning technology, an abnormal development detection model is trained in a cross-institution mode, embryo abnormal development behaviors are detected based on adversarial training, and an evaluation result is adjusted; and based on a self-adaptive ladder evaluation mechanism, dynamically adjusting an evaluation standard according to embryonic development characteristics and historical records, and calculating a final quality score. The method can comprehensively extract the multi-dimensional dynamic characteristics of the embryo, optimize the evaluation strategy and accurately detect abnormal development.

The present disclosure provides a method for promoting in vitro development of mammalian embryos, comprising the step of adding alpha-ketoglutarate in the embryo development fluid at 90-180h after in vitro fertilization. Compared with the method of adding throughout the whole embryo development, the present disclosure finds that the promotion of embryo development efficiency is more significant when alpha-ketoglutarate is added after 90 hours after fertilization (90 hours to 180 hours after fertilization).

The invention provides an embryonic development evaluation method and device, electronic equipment and a storage medium, and relates to the technical field of assisted reproduction. The method comprises the following steps: acquiring an embryo image of a to-be-evaluated embryo, and extracting an inner cell mass image from the embryo image; traversing the internal cell cluster image, and determining a pixel distribution index corresponding to the internal cell cluster image; determining at least one feature parameter of the internal cell mass image according to a pixel distribution index corresponding to the internal cell mass image; and determining an evaluation result of the to-be-evaluated embryo according to each characteristic parameter of the inner cell mass image. According to the method, pixel traversal is carried out on an extracted internal cell cluster image to obtain position indexes of a plurality of center pixel points so as to obtain a pixel distribution index, and feature mining is carried out based on pixel relative position difference information indicated by the pixel distribution index so as to obtain multiple feature parameters capable of representing texture information of the internal cell cluster image. Therefore, the method is used for evaluating embryonic development, and the accuracy and credibility of an evaluation result are improved.

The invention discloses application of a porphyromonas gingivalis extract in preparation of products related to an embryo cleft palate model, relates to the technical field of molecular biology, and particularly discloses application of the porphyromonas gingivalis extract in construction of the embryo cleft palate model. The research finds that in the embryonic development period, after a porphyromonas gingivalis extract is applied to a parent body by simulating the condition that a periodontitis patient is infected with the porphyromonas gingivalis, a certain probability of cleft palate of an embryo can be caused, and then the application of the porphyromonas gingivalis to construction of related products of an embryo cleft palate model is provided. The invention provides a novel method for constructing a cleft palate embryo, the application field of the porphyromonas gingivalis is expanded, the research on periodontal diseases and systemic toxic and side effects caused by oral infection of the porphyromonas gingivalis is deepened, and a theoretical basis is provided for the occurrence and prevention research of offspring cleft palate of maternal periodontitis patients.

The invention relates to the technical field of biology, and discloses a method for constructing a drosophila embryo permeabilization model, which comprises the following steps: collecting and cleaning drosophila embryos: collecting and washing the drosophila embryos; rinsing the drosophila melanogaster embryos, namely rinsing the drosophila melanogaster embryos by using a bleaching agent solution to remove chorion layers of the drosophila melanogaster embryos, and washing the embryos after rinsing; and performing permeation treatment on the drosophila melanogaster embryo: performing permeation treatment on the drosophila melanogaster embryo without the chorion layer in an embryo permeation solution EPS, and then cleaning in BPS to obtain the drosophila melanogaster embryo permeation model. According to the construction method of the drosophila melanogaster embryo permeabilization model, operation is easy, convenient and rapid, it can be guaranteed that embryo permeability is good, embryo vitality is maintained at the same time, the influence of small molecule drugs on the embryo development process is conveniently studied, and the method is suitable for popularization and application. And favorable conditions are provided for researching cell and molecular mechanisms of embryonic development, toxicological evaluation of small molecular substance screening and screening of related drugs in an embryo model.

The invention belongs to the technical field of bioengineering, and particularly relates to application of RPS26 recombinant protein in promoting early embryonic development of animals. Experiments show that after the RPS26 recombinant protein is added in the early-stage in-vitro embryo culture process of the lamb goats, the blastocyst rate of the lamb goats can be increased to 40.0%; meanwhile, the cell proportion of cell mass in the blastocyst can be remarkably increased, the cell proportion of trophoderm is reduced, and the total number of blastocyst cells is not influenced, so that the blastocyst development rate can be remarkably increased and the blastocyst quality can be improved by utilizing the RPS26 recombinant protein.

To provide a method for culturing avian embryos outside the eggshell that can reliably achieve high embryo survival rates and low embryonic developmental abnormality rates, and that is relatively easy to perform, as well as a method for evaluating the toxicity of test substances using such a culture method. [Solution] The embryo and yolk are removed from the fertilized bird egg and transferred together with avian aqueous oocyte white into a roughly cylindrical well in a culture vessel. The opening of the well, which is filled with avian aqueous oocyte white, is then covered with a porous membrane, and the avian embryo is cultured by turning it over. Preferably, the cultured avian embryo is further cultured in a roughly cylindrical well in which a porous membrane is provided along the inner circumference, and the amount of avian aqueous oocyte white is adjusted so that there is a space between the well and the avian yolk below the opening.

The application relates to the technical field of embryo development dynamic evaluation, and discloses an assisted reproduction management method and system. The method comprises the following steps: acquiring continuous biological state records of an in-vitro development cycle; analyzing and generating a biological state multidimensional space-time sequence; performing contour identification and dynamic tracking, and constructing a morphological trajectory; defining anchor points on the trajectory and generating a set of quantitative descriptors; performing space-time registration on the descriptor set and a development stage reference template, and establishing a mapping relationship; recombining the descriptors into a graph structure according to the mapping relationship; performing hierarchical feature mining on the graph structure, and extracting deep development mode features; inputting the deep features into a trajectory simulator to deduce an expected development trajectory; evaluating the expected trajectory, and outputting a development evaluation report. The application realizes continuous dynamic quantification and deep mode mining of the whole embryo development process, and improves the objectivity and prediction accuracy of development evaluation.

The invention relates to the technical field of artificial breeding of wild animals, in particular to an artificial hatching method for green peacocks. The method mainly comprises the steps of breeding cage building, parent selection and matching, hatching egg management, hatching environment control, embryonic development monitoring, hatching management and brooding management modules, and the hatching rate and the nestling survival rate are remarkably increased by simulating the native habitat environment, optimizing the hatching egg treatment process, precisely regulating and controlling hatching parameters and conducting brooding management in stages. According to the method, parents are screened by adopting a molecular marker technology, inbreeding mating is avoided, and the problem of embryo heat stress is relieved by innovatively combining egg cooling operation. The system is suitable for protecting endangered wild animals and provides a reference example for artificial breeding of rare pheasants.

The invention relates to the technical field of embryonic development evaluation, and discloses an embryonic chromatin openness and gene expression evaluation method, which comprises the following steps: extracting an embryonic cell sample with a survival rate of more than or equal to 95% through a micromanipulation technology; acquiring chromatin open signal data by adopting an ATAC-seq technology; carrying out genome positioning by utilizing a BWA comparison tool, and constructing a three-dimensional contact matrix through a matrix decomposition algorithm in combination with Hi-C data; determining the gene expression quantity by adopting fluorescent quantitative PCR (Polymerase Chain Reaction); and constructing an XGBoost integrated learning model to fuse chromatin characteristics and gene expression data. By optimizing micromanipulation parameters and an ATAC-seq experimental process, the cell survival rate is increased to 95.2 + / -1.3%, the ATAC-seq library complexity reaches 85.3%, and Hi-C data standardization processing is combined, so that the technical problem of data deviation is solved, and the embryo sample processing efficiency and data reliability are remarkably improved.

The invention provides an ART offspring circulatory system defect prediction method and system and a storage medium. The method comprises the following steps: acquiring parental clinical data, gene data and embryo monitoring image data of a to-be-predicted ART offspring; extracting an embryonic development event time node based on the image data, calculating a deviation with a preset standard model, generating a multi-scale space-time distortion parameter matrix, and converting the multi-scale space-time distortion parameter matrix into a development asynchronism scalar; analyzing parental data by using a circulatory system defect related gene-clinical index knowledge graph, constructing a weighted feature network, developing an asynchronous scalar to adjust an edge weight, and calculating initial feature propagation centrality of each node; inputting the space-time distortion parameter matrix into an embryo channel of a double-flow interactive attention network, and inputting the initial feature propagation centrality and other parental clinical data into a parental channel; and fusing the two-channel features through an interactive attention layer to generate context sensing fusion features, and outputting the risk probability of occurrence of circulatory system defects of ART offspring based on the features.

The invention relates to a culture medium and a method for obtaining extraembryonic endoderm stem cells (XEN) from porcine blastocysts and embryonic stem cells (ESCs), and belongs to the technical field of biology. The invention develops a two-step induction method for directly converting pig ESCs into pig XEN without gene modification and two culture mediums 4FY and 2FYSC. The invention provides a high-quality model for exploring separation characteristics and intercellular communication of embryos and extraembryonic pedigree in early embryonic development of large animals, provides a good cell model for researching dynamic development of extraembryonic pedigree, and provides a high-quality material for regenerative medicine and agricultural breeding.

The invention discloses a kit for predicting the development state of a bovine embryo and a CNVR combination used by the kit. The kit comprises a product for detecting the expression quantity and / or copy number of the CNVR combination in the bovine embryo genome; the CNVR combination comprises CNVR-Vst5, CNVR-Vst11, CNVR-Vst7 and / or CNVR-Vst4, and the CNVR-Vst5, the CNVR-Vst11, the CNVR-Vst7 and the CNVR-Vst4 If the expression quantities and / or copy numbers of CNVR-Vst5, CNVR-Vst11, CNVR-Vst7 and / or CNVR-Vst4 in a bovine embryo genome subjected to in-vitro fertilization culture are obviously different from those of a normal bovine embryo, the development of the bovine embryo is predicted to be stagnated, and embryo implantation is not suggested. The method can be used for scientifically judging the transplantation strategy and decision of the bovine embryo, and has important application value.