130 results about "Prenatal development" patented technology

The invention relates to the technical field of biomedicine detection reagent manufacturing and reproductive medicine, in particular to an embryonic development potential sperm key protein kit and a use method thereof. The method is characterized in that an antibody combination capable of specifically recognizing a group of key proteins related to embryonic development potential in human sperms is prepared, and the antibodies, a fluorescence labeled secondary antibody, a cell nucleus dye, a concentrated washing liquid, a mounting medium and a pre-coated control porous glass slide are assembled into the special detection kit. The kit can be combined with a multi-mode imaging system (such as a fluorescence / confocal microscope and a common optical microscope) and image analysis software to realize the traditional analysis of sperm morphology and movement ability, and can perform synchronous and accurate qualitative and quantitative analysis with the expression level and subcellular localization of the key protein. The invention provides an integrated molecular detection solution, and provides a powerful tool for sperm quality screening and embryonic development potential prediction in an assisted reproduction technology.

The invention discloses a bovine oocyte in-vitro maturation culture solution as well as a preparation method and application thereof, and belongs to the technical field of animal reproduction and embryo engineering. The preparation method of the bovine oocyte in-vitro maturation culture solution comprises the step of adding mitoquinine mesylate into a conventional IVM culture solution, wherein the adding concentration of the mitoquinine mesylate is preferably 50 nmol / L. The bovine oocyte in-vitro maturation culture solution provided by the invention can effectively reduce the oxidative stress reaction of the oocytes in the in-vitro culture process, enhance the mitochondrial function and remarkably improve the bovine oocyte in-vitro maturation quality and the embryonic development potential, so that the blastocyst rate is increased, and the problem that the development potential of the existing oocyte IVM culture solution is insufficient is solved.

The presently disclosed subject matter relates to using a haploid inducing line (whether existing or created) and transforming the haploid line so that it encodes cellular machinery capable of editing genes. The transformed haploid inducing line is used as a parent in a cross between two plants. During pollination, the parental gametes fuse to form an embryo; and the gene editing machinery is also delivered to the embryo at this time. During embryonic development, one set of parental chromosomes are lost, and the gene editing machinery operates on the remaining set of chromosomes. Thus, at least one haploid progeny with edited genes is produced from the cross.

This invention relates to the interdisciplinary field of assisted reproductive technology and artificial intelligence, specifically to a system and method for constructing an embryonic developmental trajectory map that integrates morphological features. By deeply integrating static morphological features with dynamic morphodynamic parameters, a more comprehensive panoramic map of embryonic development with richer information dimensions is constructed, overcoming the one-sidedness of single-dimensional assessment. Automated analysis of the map using an AI model reduces the influence of subjective human factors, enabling the discovery of subtle developmental patterns and potential differences that are difficult to discern with the human eye, significantly improving the accuracy of selecting high-quality embryos. The provided developmental trajectory map allows embryologists to intuitively examine the entire developmental process of the embryo and the performance of each key node, enhancing the interpretability and credibility of the assessment process. Through more scientific embryo selection, it is expected to improve embryo implantation and clinical pregnancy rates, and reduce multiple pregnancy and miscarriage rates.

Provided are a reagent combination or a kit for constructing an embryoid and the use thereof. The reagent combination or the kit can be used for inducing a stem cell to produce a blastocyst-like lineage precursor cell, a blastocyst-like cell and / or an embryoid, which are based on small-molecule induction and independent of transgenes, and have a single cell source. The produced blastocyst-like lineage precursor cell and blastocyst-like cell have a full blastocyst lineage, and are seed cells having balanced developmental competence and totipotency, which solves the problem of unbalanced cell maturation in the prior art. The produced embryoid is highly similar to natural embryos in terms of morphological characteristics and transcriptomic characteristics, has the capability of highly reproducing post-implantation embryonic development and / or gastrulation, which is beneficial for research on embryonic development in vitro. The method for inducing the stem cell to produce the blastocyst-like lineage precursor cell, the blastocyst-like cell and / or the embryoid by using the reagent combination or the kit is simple, and has high applicability and high embryoid construction efficiency.

The invention relates to the technical field of embryo quality evaluation, and discloses an embryo development quality evaluation method based on image recognition. The method comprises the following steps: acquiring an embryonic development image sequence, and judging whether the data volume reaches a preset acquisition threshold value; if a threshold value is reached, constructing an embryo feature vector, and processing the embryo feature vector by a multi-dimensional feature dynamic encoder to obtain optimized feature data; a dynamic game-reinforcement learning framework is adopted, and a development quality evaluation strategy is optimized through interaction of an evaluation agent and an embryo population agent; data collaboration is realized by means of a federated learning technology, an abnormal development detection model is trained in a cross-institution mode, embryo abnormal development behaviors are detected based on adversarial training, and an evaluation result is adjusted; and based on a self-adaptive ladder evaluation mechanism, dynamically adjusting an evaluation standard according to embryonic development characteristics and historical records, and calculating a final quality score. The method can comprehensively extract the multi-dimensional dynamic characteristics of the embryo, optimize the evaluation strategy and accurately detect abnormal development.

The present disclosure provides a method for promoting in vitro development of mammalian embryos, comprising the step of adding alpha-ketoglutarate in the embryo development fluid at 90-180h after in vitro fertilization. Compared with the method of adding throughout the whole embryo development, the present disclosure finds that the promotion of embryo development efficiency is more significant when alpha-ketoglutarate is added after 90 hours after fertilization (90 hours to 180 hours after fertilization).

The invention relates to the technical field of biology, and discloses a method for constructing a drosophila embryo permeabilization model, which comprises the following steps: collecting and cleaning drosophila embryos: collecting and washing the drosophila embryos; rinsing the drosophila melanogaster embryos, namely rinsing the drosophila melanogaster embryos by using a bleaching agent solution to remove chorion layers of the drosophila melanogaster embryos, and washing the embryos after rinsing; and performing permeation treatment on the drosophila melanogaster embryo: performing permeation treatment on the drosophila melanogaster embryo without the chorion layer in an embryo permeation solution EPS, and then cleaning in BPS to obtain the drosophila melanogaster embryo permeation model. According to the construction method of the drosophila melanogaster embryo permeabilization model, operation is easy, convenient and rapid, it can be guaranteed that embryo permeability is good, embryo vitality is maintained at the same time, the influence of small molecule drugs on the embryo development process is conveniently studied, and the method is suitable for popularization and application. And favorable conditions are provided for researching cell and molecular mechanisms of embryonic development, toxicological evaluation of small molecular substance screening and screening of related drugs in an embryo model.

The invention belongs to the technical field of bioengineering, and particularly relates to application of RPS26 recombinant protein in promoting early embryonic development of animals. Experiments show that after the RPS26 recombinant protein is added in the early-stage in-vitro embryo culture process of the lamb goats, the blastocyst rate of the lamb goats can be increased to 40.0%; meanwhile, the cell proportion of cell mass in the blastocyst can be remarkably increased, the cell proportion of trophoderm is reduced, and the total number of blastocyst cells is not influenced, so that the blastocyst development rate can be remarkably increased and the blastocyst quality can be improved by utilizing the RPS26 recombinant protein.

To provide a method for culturing avian embryos outside the eggshell that can reliably achieve high embryo survival rates and low embryonic developmental abnormality rates, and that is relatively easy to perform, as well as a method for evaluating the toxicity of test substances using such a culture method. [Solution] The embryo and yolk are removed from the fertilized bird egg and transferred together with avian aqueous oocyte white into a roughly cylindrical well in a culture vessel. The opening of the well, which is filled with avian aqueous oocyte white, is then covered with a porous membrane, and the avian embryo is cultured by turning it over. Preferably, the cultured avian embryo is further cultured in a roughly cylindrical well in which a porous membrane is provided along the inner circumference, and the amount of avian aqueous oocyte white is adjusted so that there is a space between the well and the avian yolk below the opening.

The application relates to the technical field of embryo development dynamic evaluation, and discloses an assisted reproduction management method and system. The method comprises the following steps: acquiring continuous biological state records of an in-vitro development cycle; analyzing and generating a biological state multidimensional space-time sequence; performing contour identification and dynamic tracking, and constructing a morphological trajectory; defining anchor points on the trajectory and generating a set of quantitative descriptors; performing space-time registration on the descriptor set and a development stage reference template, and establishing a mapping relationship; recombining the descriptors into a graph structure according to the mapping relationship; performing hierarchical feature mining on the graph structure, and extracting deep development mode features; inputting the deep features into a trajectory simulator to deduce an expected development trajectory; evaluating the expected trajectory, and outputting a development evaluation report. The application realizes continuous dynamic quantification and deep mode mining of the whole embryo development process, and improves the objectivity and prediction accuracy of development evaluation.

The invention relates to the technical field of artificial breeding of wild animals, in particular to an artificial hatching method for green peacocks. The method mainly comprises the steps of breeding cage building, parent selection and matching, hatching egg management, hatching environment control, embryonic development monitoring, hatching management and brooding management modules, and the hatching rate and the nestling survival rate are remarkably increased by simulating the native habitat environment, optimizing the hatching egg treatment process, precisely regulating and controlling hatching parameters and conducting brooding management in stages. According to the method, parents are screened by adopting a molecular marker technology, inbreeding mating is avoided, and the problem of embryo heat stress is relieved by innovatively combining egg cooling operation. The system is suitable for protecting endangered wild animals and provides a reference example for artificial breeding of rare pheasants.

The invention provides an ART offspring circulatory system defect prediction method and system and a storage medium. The method comprises the following steps: acquiring parental clinical data, gene data and embryo monitoring image data of a to-be-predicted ART offspring; extracting an embryonic development event time node based on the image data, calculating a deviation with a preset standard model, generating a multi-scale space-time distortion parameter matrix, and converting the multi-scale space-time distortion parameter matrix into a development asynchronism scalar; analyzing parental data by using a circulatory system defect related gene-clinical index knowledge graph, constructing a weighted feature network, developing an asynchronous scalar to adjust an edge weight, and calculating initial feature propagation centrality of each node; inputting the space-time distortion parameter matrix into an embryo channel of a double-flow interactive attention network, and inputting the initial feature propagation centrality and other parental clinical data into a parental channel; and fusing the two-channel features through an interactive attention layer to generate context sensing fusion features, and outputting the risk probability of occurrence of circulatory system defects of ART offspring based on the features.

This invention discloses a method and system for embryo quality detection, relating to the field of embryo detection technology, including the following steps: S1: fertilized embryos are continuously cultured in an in vitro culture system containing specific metabolic marker detection components, and continuous dynamic imaging of the embryos is performed to obtain a complete dynamic developmental image sequence; S2: the dynamic developmental image sequence is segmented, and temporal developmental features are captured, identifying key time points such as pronucleus appearance and disappearance, cleavage, and blastocyst formation, and generating a segmentation mask corresponding to each frame of the dynamic developmental image sequence; this invention achieves full-process time-difference imaging to capture the temporal features of embryonic development, without trauma or interference, avoiding subjective bias in static observation; by segmenting the dynamic developmental image sequence and capturing temporal developmental features, the segmentation accuracy is high and the anti-interference ability is strong.

This invention relates to the field of bioengineering technology, and in particular to a method for simultaneously collecting the inner cell mass and trophectoderm of mammalian blastocysts. The method includes the following steps: (1) after removing the zona pellucida of the blastocyst, the blastocyst is cut into an inner cell mass and a trophectoderm portion, and the cut trophectoderm portion is transferred to a culture medium for collection; (2) the inner cell mass portion obtained in step (1) is sequentially placed in an antibody treatment solution and a complement treatment solution for incubation; (3) the inner cell mass portion treated in step (2) is pipetted to obtain a purified inner cell mass. This method achieves simultaneous and efficient separation of two key cell lines from the blastocyst, fundamentally changing the traditional method's "resource waste" model of only obtaining the inner cell mass while discarding the trophectoderm, and providing more comprehensive experimental materials for embryonic development, placental biology, and stem cell research.

The invention discloses a construction method and application of an animal model with delayed embryonic development, and relates to the technical field of animal models.The construction method of the animal model with delayed embryonic development comprises the following steps that S1, an early embryo is obtained; s2, performing contact culture on the early embryo and a development retardation inducer; s3, culturing to obtain a slowly developed embryo; the proteomics analysis method based on non-data-dependent acquisition is used for analyzing an animal model with slow embryonic development. The method comprises the following steps: providing an embryo sample; obtaining a protein expression profile by adopting a non-data-dependent acquisition proteomics method; according to a protein expression profile, identifying differential expression proteins related to the embryonic development retardation; the animal model with the delayed embryonic development is constructed, and an experimental model is provided for researching a molecular mechanism of the delayed embryonic development; a non-data-dependent collection proteomics analysis method is applied to the animal model, and compared with a traditional data-dependent collection method, the method has higher stability.

This invention discloses an AI-based personalized dynamic embryo culture system, belonging to the field of embryo culture technology. The system adopts a modular architecture, including a core control module, at least one independent culture chamber module, an AI analysis module, and a dynamic regulation module. By employing microfluidic chip technology and an integrated microsensor array, this invention achieves high-precision real-time monitoring of embryo morphology images and physiological parameters of the culture medium. Combined with multimodal data fusion analysis using the AI ​​analysis module, it significantly improves the accuracy and reliability of embryo developmental potential assessment, thereby contributing to increased rates of high-quality embryos and clinical pregnancy success. Furthermore, this invention, through a built-in personalized protocol generation unit, can dynamically generate and execute personalized culture protocols based on the patient's age, ovarian function indicators, and basal hormone levels, while utilizing the dynamic regulation module to simulate the uterine diurnal rhythm for temperature fluctuation control.

The invention discloses a strain of Ligilbacillus murinus PV211184 capable of improving the reproductive performance of livestock, which belongs to the field of agricultural animal husbandry, is preserved in Guangdong Province Microbial Culture Collection Center, has a preservation number of GDMCC No: 66039, has a bacterial colony in milk white forms such as round bulge, smoothness and the like, has a suitable growth temperature of 30-45 DEG C, a pH value of 4.0-6.5, preferably 37 DEG C and a pH value of 6.0, and is subjected to facultative anaerobic culture by using an MRS culture medium. The bacterium can be colonized in the uterus, has good endometrial epithelial cell adhesion capability, has no negative influence on sperm motility and IVF embryonic development, and can promote endometrial epithelial cell proliferation. Experiments show that the litter size per litter of a sow can be increased by 1.3, the litter size per litter can be increased by 1.0, the lambing rate of Hu sheep can be increased by 7.2%, the lambing rate per litter can be increased by 14.5%, and the feed can be used as a probiotic agent for improving the reproductive performance of livestock.

The invention relates to the technical field of artificial intelligence in assisted reproduction, in particular to an embryo optimization model construction method, device and equipment and a storage medium. Comprising the following steps: acquiring video data of embryonic development from a plurality of fertilized eggs to a blastocyst stage based on a delayed photography embryo culture technology; constructing time sequence image data according to the video data; the time sequence image data comprises multiple frames of continuous images; performing feature extraction on each frame of image of the time sequence image to obtain a high-dimensional feature corresponding to each frame of image; determining a comparison difference feature with the corresponding reference sample feature, performing fusion, and constructing a fusion feature corresponding to each frame of image; constructing a feature sequence of the target embryo development according to the fusion features; and acquiring a trained embryo quality grading model according to a time dependency relationship of the feature sequence for capturing embryo development. The model provided by the invention can optimize the utilization rate of embryos, reduce unnecessary repeated ovulation promoting cycles, and promote the development of reproductive medicine towards the precision direction.

Provided is an embryo model, which is obtained by means of self-assembly of induced pluripotent stem cells and induced hypoblast stem cells, wherein both the induced pluripotent stem cells and the induced hypoblast stem cells are obtained by means of somatic cell reprogramming. The self-assembly of the stem cells of two different lineages generated by means of somatic cell reprogramming forms an embryo model simulating human peri-gastrulation. The embryo model better reproduces the interaction and communication mechanism among cells of multiple lineages during human embryonic development by means of combining cells of different lineages. With the embryo model, by means of a gene editing tool, lineage tracing and gene regulatory network research can be performed on cells of different lineages, thereby enabling an in-depth understanding of the molecular regulatory mechanisms of early human embryonic development.

The invention provides a breeding method for improving the breeding rate of replacement gilts, and the breeding method comprises the following steps: (1) adding a first functional nutritional composition into feed from 130-150 days old to the early estrus of the replacement gilts; (2) adding a second functional nutrient composition into the feed from the initial estrus period of the replacement gilts to the time before mating; and (3) within 25-35 days after mating of the replacement gilts, adding a second functional nutrient composition into the feed. According to the breeding method, oxidative stress of the replacement gilts can be relieved, development and maturation of follicles can be adjusted, oestrus and ovulation of the gilts can be promoted, the initial estrus period of the replacement gilts can be started in time, embryonic development can be promoted, the conception rate of the replacement gilts can be increased, and the replacement gilts are prevented from being eliminated due to delayed oestrus or non-oestrus or repeated breeding and infertility after oestrus; further, the breeding rate of the replacement gilts is improved.

The invention discloses application of the ratio of phosphatidylserine to taurocholic acid. Research finds that compared with assisted reproduction crowds capable of obtaining available embryos after embryo culture, the concentration of phosphatidylserine in follicular fluid of assisted reproduction crowds without available embryos after embryo culture is remarkably reduced, which prompts that phosphatidylserine can be used as a marker for predicting developmental potential of assisted reproduction embryos; the prediction module is used for predicting whether available embryos can be obtained or not in advance in an egg taking stage of assisted reproduction; and the ROC curve verifies that the AUC of the PS (21: 220: 4) is 0.741, and the AUC of the ratio of the PS (21: 220: 4) to the taurocholic acid is 0.784, so that the accuracy and the sensitivity are higher when the ratio of the PS (21: 220: 4) to the taurocholic acid is used as the marker to predict whether available embryos exist or not.

The invention provides a rainbow trout recirculating aquaculture, spawning and hatching integrated device, and belongs to the technical field of aquaculture, the rainbow trout recirculating aquaculture, spawning and hatching integrated device comprises an aquaculture device body, a vibration turbulent flow assembly and a circulating air supply assembly; through cooperation of the generation chamber, the incubation chamber and the outlet cavity and a hydraulic transfer system, connection from spawning, fertilization to incubation of rainbow trout is achieved, ovum transfer damage is reduced, and the initial incubation rate is increased; a shading structure is designed to shield external strong light and protect embryonic development; the filter screen is matched with the directional nozzle, and the vibration turbulent flow assembly is combined, so that self-cleaning of the filter screen is achieved, pollution sources are directionally discharged, and hatching water is kept clean; according to the circulating air supply assembly, air is continuously supplied to the upper portion of water in the incubation chamber through a rotating wheel, bubbles are pressed downwards through water flow sprayed obliquely downwards, mixed dive flow is formed, oxygenation can be achieved without extra electric power, the water is stirred through the generated bubbles, the temperature and dissolved oxygen in the incubation chamber are distributed more evenly, and the incubation environment is further optimized.

The invention discloses a leuciscus waleckii embryo hatching solution, a preparation method thereof and a method for improving the hatching rate of leuciscus waleckii embryos, and the leuciscus waleckii embryo hatching solution is a hatching mother solution formed by dissolving 170-180g of sodium chloride, 20-30g of potassium chloride, 4-6g of calcium chloride and 0.4-0.6 g of methylene blue in distilled water and fixing the volume to 1L; the invention further specifically discloses a preparation method of the leuciscus waleckii embryo hatching liquid and application of the leuciscus waleckii embryo hatching liquid in increasing the hatching rate of leuciscus waleckii embryos. According to the hatching working solution, the hatching mother solution is prepared and used immediately, water pollution is avoided, water dissolved oxygen is increased, the embryo development environment is stabilized, invasion of saprolegniasis spores and other bacteria can be inhibited under the low-temperature condition, saprolegniasis outbreak is inhibited, the hatching rate and the survival rate of leuciscus waleckii embryos are increased, and the survival rate of leuciscus waleckii embryos is increased. And a reliable technical scheme is provided for realizing large-scale and standardized artificial propagation of the leuciscus waleckii.

The invention discloses a biomarker combination based on pollutant exposure detection and application thereof, and relates to the technical field of biomarkers, the biomarker combination comprises an integrin subunit itg-alpha11, a nuclear transcription factor nf-kb, and an antioxidant stress factor nrf2; according to the biomarker combination disclosed by the invention, a complete association network from molecular response to phenotypic risk is constructed by accurately capturing an action mechanism of BDE-47 at molecular and cellular levels; the rapid diagnosis of pollutant exposure can be realized in the early stage of embryonic development, and the risk of development malformation and the like can be effectively early warned; and meanwhile, a quantitative basis is provided for evaluating the pollution remediation effect, and accurate formulation of an environment quality standard and optimization of an aquaculture management strategy are supported. The biomarker combination not only deepens the theoretical cognition of a pollutant toxicity mechanism, but also provides an innovative tool for environmental health risk assessment and ecological protection practice, and has important theoretical significance and wide application prospects.

The invention belongs to the technical field of assisted reproduction, and particularly relates to a biological response type memristor for in-vitro embryo monitoring and application of the biological response type memristor. The memristor comprises a substrate, a bottom electrode, a dielectric layer and a top electrode which are sequentially arranged from bottom to top, the dielectric layer is a double-layer functional layer composed of an Nb2O5 thin film and a barium titanate thin film, the barium titanate thin film is located on the upper layer of the bottom electrode, and the Nb2O5 thin film is located on the upper layer of the barium titanate thin film. The memristor realizes response to biological information by monitoring metabolic byproducts and oxidative stress markers in an embryo in-vitro culture solution in real time, realizes operation by inducing redistribution of surface charges of an interface through ion adsorption, and shows a resistance state transformation characteristic. Besides, the system breaks through the limitation of traditional microscope visual evaluation, realizes direct, sensitive and quantitative electric signal reading of the embryo metabolism state and the oxidative stress level, and solves the problem of embryo development potential evaluation caused by the increase of aneuploid rate in old lying-in women and recurrent implantation failure groups.

The invention belongs to the technical field of biology, and discloses a DNA tetrahedral probe for live oocyte mitochondrial ATP imaging and application of the DNA tetrahedral probe. The invention provides a DNA tetrahedron delivery-based nucleic acid probe for fixed-point trigger cascade nucleic acid reaction, which can realize high-sensitivity and rapid detection of mitochondrial ATP of living oocytes. The nucleic acid probe is stable in structure and high in specificity, can accurately distinguish similar 12s rRNA and ATP analogues (such as CTP, GTP and UTP), remarkably reduces false positive signals, and ensures the reliability of detection results. The nucleic acid probe realizes ATP in-situ imaging at a mitochondrial level, is helpful for deep evaluation of an oocyte energy state, provides key indexes for quality reduction of oocytes of elderly women and evaluation of embryonic development potential, and has positive significance in improvement of assisted reproductive outcome.

Stabilized amorphous calcium carbonate (ACC) for treatment of several neurological, muscular and infertility diseases and conditions is provided. In particular, the stabilized ACC may be used in the treatment of axonal defects and muscular dystrophy. In addition, provided are improved methods used in assistant reproductive technology. Examples of such methods are in vitro fertilization and improvement of sperm quality. The improved IVF method, for example, comprises addition of the stabilized ACC to the cell culture medium in which the stages of fertilization and embryo development occurs.

The presently disclosed subject matter relates to using a haploid inducing line (whether existing or created) and transforming the haploid line so that it encodes cellular machinery capable of editing genes. The transformed haploid inducing line is used as a parent in a cross between two plants. During pollination, the parental gametes fuse to form an embryo; and the gene editing machinery is also delivered to the embryo at this time. During embryonic development, one set of parental chromosomes are lost, and the gene editing machinery operates on the remaining set of chromosomes. Thus, at least one haploid progeny with edited genes is produced from the cross.