33 results about "Pregnancy rate" patented technology

The invention relates to the technical field of animal husbandry, in particular to a synchronous oestrus-timing insemination method suitable for cattle, which comprises the following steps: firstly, performing intramuscular injection of PG (cloprostenol sodium) at different time points after calving to promote uterine recovery and luteolysis and lay a synchronous oestrus foundation; the method comprises the key steps that GnRH (gonadotropin release hormone) is given through intramuscular injection on the 43rd day, the 53rd day, the 60th day and the 69th day, pituitary is promoted to release follicle-stimulating hormone (FSH) and luteinizing hormone (LH), collection and screening of follicles are guided, follicle maturation is promoted, the oestrus uncovering rate and the oestrus conception rate are increased, a melatonin implant is implanted in a subcutaneous implantation mode on the 63rd day, and the melatonin implant is implanted in a subcutaneous implantation mode on the 63rd day. The synthesis of ovarian hormones is promoted by regulating the hypothalamus-pituitary-gonad axis, the secretion of follicle cell factors and maturation promoting factors is promoted, the development of granular cells is promoted, and the maturation of oocytes is promoted. According to the method, the pregnancy rate of a double-synchronization-timing insemination scheme which is most widely used in a large-scale dairy cow breeding pasture at present can be remarkably increased, and feasible technical support is provided for success of increasing the breeding efficiency of cows.

The application discloses an antioxidant combined diluent for improving the freezing preservation effect of sheep semen and application thereof. The mitochondrion-targeted antioxidant SS-31 is combined with the plant-derived antioxidant CUR for the freezing preservation of sheep semen, so that the generation and action of peroxides can be inhibited, the antioxidant capacity of sperm is enhanced, oxidative stress damage can be effectively avoided, the quality of frozen sheep semen is improved, the fertilization capacity is maintained or improved, and the pregnancy rate is improved.

The invention discloses a traditional Chinese medicine composition for treating PCOS pregnancy loss and application of the traditional Chinese medicine composition. The traditional Chinese medicine composition comprises the following raw material medicines in parts by weight: 20-40 parts of salted semen cuscutae, 20-40 parts of prepared rehmannia root, 10-20 parts of scutellaria baicalensis, 10-20 parts of dogwood, 20-30 parts of astragalus membranaceus, 20-30 parts of bighead atractylodes rhizome, 20-30 parts of white paeony root, 10-20 parts of parasitic loranthus and 10-20 parts of teasel According to the invention, active component groups (mass spectrum identification) and accurate compatibility design (kidney tonifying and miscarriage prevention theory) are defined, so that (1) the PCOS pregnancy rate is obviously increased (52%-80%, experimental verification); (2) synchronously improving reproduction-metabolism-uterus / placenta triple defects (data support such as a < HOMA-IR <->, a < Total T <->, a < Hif1 alpha > <-> and the like); and (3) the blank in the prior art is filled, and an integrated treatment scheme with both embryo protection and placenta function repair is provided. Experimental data, a molecular mechanism and traditional Chinese medicine theories prove the innovativeness and clinical value of the traditional Chinese medicine composition.

The application discloses a mesenchymal stem cell preparation and application thereof in thin endometrium repair, and comprises the following steps: adding Enterococcus durans CGMCC 1.3249 and Bacillus subtilis CGMCC 1.15792 supernatant as effective components into cultured mesenchymal stem cells; and the stem cell preparation obtained by the method can effectively restore the endometrial thickness of mice, increase the number of glands, and improve the pregnancy rate of the thin endometrium mice.

The invention belongs to the technical field of animal breeding, and particularly relates to application of alpha-ketoglutaric acid or salt thereof in preparation of a sheep semen cryopreservation diluent. The invention discloses the application of alpha-ketoglutaric acid or salt thereof in the cryopreservation of the sheep semen for the first time, and the alpha-ketoglutaric acid or salt thereof can improve the semen quality of the frozen and unfrozen sheep semen and improve the fertilization ability of the frozen semen; such as sperm motility, sperm movement performance, sperm viability, sperm plasma membrane integrity rate, sperm acrosome integrity rate, sperm antioxidant capacity, sperm ATP level, sperm early apoptosis incidence rate, sperm in-vitro fertilized ovulation rate and / or artificial fertilization pregnancy rate, can be used for preparing the sheep sperm cryopreservation diluent.

The invention belongs to the technical field of traditional Chinese medicines, and particularly relates to a traditional Chinese medicine composition for treating polycystic ovarian syndrome as well as a preparation method and application thereof. The traditional Chinese medicine composition is prepared from Chinese yam, semen cuscutae, pulp of dogwood fruit, radix glehniae, radix scrophulariae, lily, rhizoma polygonati, tortoise shell, cistanche deserticola, lotus seed and raspberry, has the effects of tonifying kidney and replenishing essence, nourishing yin and blood, replenishing qi and tonifying spleen, and benefiting uterus and accommodating fetus, and is mainly used for treating the polycystic ovarian syndrome. Research results show that the traditional Chinese medicine composition can significantly improve the sex hormone level of a patient with the polycystic ovarian syndrome, increase the endometrial thickness, improve the ovarian function and improve the endometrial receptivity, the ovulation rate and the pregnancy rate. Therefore, the traditional Chinese medicine composition has important clinical significance and wide market application prospects.

The application discloses high-adhesion modified mesenchymal stem cells and application thereof in preparation of a medicine for treating intrauterine adhesion. The modified mesenchymal stem cells are genetically engineered to overexpress integrin alpha 2 subunit and / or integrin alpha 5 subunit, thereby enhancing the adhesion capacity to the extracellular matrix (collagen and fibronectin) of the endometrium. In vitro experiments show that the initial adhesion rate, liquid flushing resistance and anoikis resistance of the modified MSCs are significantly better than those of common MSCs. When used in combination with the "dynamic culture and drug delivery device for intrauterine adhesion treatment" applied by the inventor on the same day, the retention rate of the modified MSCs in the simulated intrauterine dynamic culture environment is significantly improved. Animal experiments show that the modified MSCs in combination with the device can significantly promote endometrial regeneration, inhibit fibrosis and improve the pregnancy rate. The application solves the core bottleneck of low cell retention rate and short survival time in the MSC transplantation treatment of intrauterine adhesion by enhancing the adhesion capacity of the MSCs, and forms a complete treatment scheme in cooperation with the device, thereby having a good clinical application prospect.

The application discloses a one-step freezing solution for bovine in-vitro embryos and a one-step freezing method for bovine in-vitro embryos and belongs to the technical field of biotechnology.The method comprises the following steps: bovine oocyte in-vitro maturation; bovine in-vitro fertilization embryo production; after culturing the bovine in-vitro fertilization embryo for 6-8 days, the embryo is moved into freezing solution A, and after standing, the shriveled blastocyst is moved from the freezing solution A into freezing solution B, the embryo sinking to the bottom of the freezing solution B is moved into freezing solution C, is loaded into a macaroni tube, and is subjected to programmed freezing; after the freezing program is completed, the macaroni tube is put into liquid nitrogen. The bovine in-vitro embryos are frozen by using the three kinds of freezing solutions, on the premise that the conventional freezing method has low technical requirements for the operator and can be directly transplanted after thawing, the conventional freezing method is overcome, the survival rate, the hatching rate and the transplantation pregnancy rate of the embryos after thawing are low, the convenience of use and the effective integration of the high transplantation pregnancy rate are realized.

The invention relates to the field of probiotics, in particular to lactobacillus enteri LLM10, a probiotic complexing agent for promoting initial start of replacement gilts and oestrus of primiparous sows and application of the probiotic complexing agent. According to the present invention, the preservation number of the Lactobacillus enteri LLM10 is CGMCC No.26850, and the Lactobacillus enteri LLM10, the cinnamon powder and the Gorgon fruit powder are prepared into the complexing agent; the complexing agent can better enable the lactobacillus enteri LLM0 to be colonized in animal bodies, and has higher tolerance to artificial cholate, artificial gastric acid and artificial intestinal juice; particularly, hormone synthesis and secretion of the replacement gilts and the primiparous sows can be better improved, so that follicular development of the replacement gilts is improved, early starting of the replacement gilts is promoted, timely oestrus of the primiparous sows after weaning is promoted, the oestrus rate is increased, the pregnancy rate is increased, and the number of live piglets is increased.

This invention provides the application of lecithin in the preparation of drugs for the prevention and / or treatment of infertility, belonging to the field of biomedical preparation technology. Specifically, it relates to the application of lecithin in the preparation of drugs for the prevention and / or treatment of infertility, recurrent miscarriage, and / or uterine decidualization disorders. The lecithin can effectively reduce TNF-α levels in early pregnancy, improve uterine decidualization disorders, and increase endometrial receptivity, thereby increasing pregnancy rates and providing a new approach for the treatment of infertility and recurrent miscarriage.

The invention relates to the field of traditional Chinese medicine, and discloses a traditional Chinese medicine composition for treating spleen and kidney deficiency type male infertility and a preparation method and application thereof. The composition consists of a pharmaceutically acceptable carrier and active ingredients, the active component is prepared from the following components in parts by weight: 10 to 15 parts of fructus cnidii, 10 to 15 parts of rhizoma curculiginis, 10 to 15 parts of fructus psoraleae, 10 to 15 parts of herba epimedii, 10 to 15 parts of semen cuscutae, 10 to 15 parts of fructus lycii, 10 to 15 parts of fructus rosae laevigatae, 10 to 15 parts of fructus rubi, 10 to 15 parts of fructus ligustri lucidi, 10 to 15 parts of tortoise shell, 15 to 20 parts of herba taxilli, 10 to 15 parts of fructus schisandrae chinensis, 10 to 15 parts of rhizoma polygonati, 15 to 20 parts of semen plantaginis and 10 to 15 parts of semen citri reticulatae. The application can obviously improve the sperm quality of a patient, improve the testosterone level of serum and increase the natural pregnancy rate, and is good in safety.

The invention belongs to the technical field of biological medicine, and particularly relates to a monoclonal antibody for detecting or blocking FomA protein with fusobacterium nucleatum as well as application and a product of the monoclonal antibody. The monoclonal antibodies obtained through screening or antigen binding fragments FAMD6 and FAME67 of the monoclonal antibodies can be specifically combined with different sites of Fn-FomA protein, an immunodetection or diagnosis kit constructed by the monoclonal antibodies or the antigen binding fragments FAMD6 and FAME67 is high in sensitivity and specificity and can rapidly and accurately detect Fn infection, the detection sensitivity can reach 3.9 ng / mL, the highest dilution of Fn culture supernatant is 1: 1600, the sensitivity is high, and the Fn infection can be rapidly and accurately detected. Good application prospects are realized. Besides, in a mouse model, the monoclonal antibody or the antigen binding fragments FAMD6 and FAME67 thereof have higher protectiveness on pregnant mice, and the bad pregnancy rate can be effectively improved.

This invention discloses a mixed cell preparation and its application in the preparation of drugs for treating premature ovarian failure. The mixed cell preparation comprises granulosa cells and mesenchymal stem cells, mixed in a certain ratio (preferably 1:1). Granulosa cells are obtained by inducing differentiation from mesenchymal stem cells and express granulosa cell-specific markers (FOXL2, FSHR, etc.), participating in follicular structure reconstruction; mesenchymal stem cells can improve the ovarian microenvironment, inhibit inflammation, and resist fibrosis, providing a suitable "soil" for the survival of granulosa cells. In vitro experiments are expected to show that mixed culture can significantly increase estradiol secretion levels and cell survival rate. Animal models of premature ovarian failure are expected to show that mixed cell therapy can significantly restore the estrous cycle, improve hormone levels, increase the number of follicles at all stages, and improve pregnancy rates, with effects significantly superior to single-cell therapy. This invention utilizes the synergistic effect of the two cell types to achieve dual repair of the "seed" and the "soil," providing a novel treatment strategy for premature ovarian failure.

The application relates to a method for preserving animal sperm. The method uses an animal sperm liquid preservation diluent to preserve animal sperm; the animal sperm liquid preservation diluent is prepared by the following method: (1) mixing glucose, fructose, tris-hydroxymethyl aminomethane, citric acid and water to obtain a mixture 1; the mass-volume ratio of the glucose, fructose, tris-hydroxymethyl aminomethane, citric acid and water is (10-20) g:(10-20) g:(20-30) g:(10-20) g:(50-100) ml; (2) mixing edible mixed vegetable oil and the mixture 1 of step (1), stirring, and the volume ratio of the edible mixed vegetable oil and water is 50:(50-100) to obtain a mixture 2; standing, discarding the upper oil, refrigerating, and diluting with sterilized distilled water. The application has the advantages of wide application range, high pregnancy rate, long animal sperm liquid preservation time, good preservation activity and the like.

The invention discloses an application of FGF12 (Fibroblast Growth Factor 12) in a medicine for improving intrauterine adhesion. A 95% ethanol-induced intrauterine adhesion rat model is constructed, and an adeno-associated virus carrying an FGF12 gene is injected into a uterine cavity for intervention, which indicates that the FGF12 can significantly activate a Rho / ROCK signal channel, further promotes phosphorylation of myosin light chain subunits on myosin light chain phosphatase, inhibits dephosphorylation of MLC, and improves the phosphorylation level of MLC in fibroblasts. The endometrial fibrosis process is inhibited, collagen deposition is remarkably reduced, the endometrial thickness and the gland number are recovered, and the TGF-beta1 expression level is improved. Meanwhile, the pregnancy rate is obviously increased, and the limitation of traditional treatment is broken through.

The invention belongs to the technical field of animal breeding, and particularly relates to application of alpha-ketoglutaric acid or salt thereof in preparation of a normal-temperature sheep semen preservation diluent. The invention discloses the application of alpha-ketoglutaric acid or salt thereof in normal-temperature preservation and / or dilution of the sheep semen for the first time, and the semen quality of the sheep semen during normal-temperature preservation and / or dilution can be improved by the alpha-ketoglutaric acid or salt thereof; such as sperm motility rate, sperm motility, sperm motility performance, sperm viability, sperm plasma membrane intact rate, sperm acrosome intact rate and / or artificial fertilization pregnancy rate, can be used for preparing the sheep sperm normal-temperature preservation diluent.

The present application relates to the technical field of pregnancy rate prediction, and particularly relates to a model establishment method and system for predicting pregnancy rate of early-onset ovarian dysfunction, which obtains clinical data of a patient, identifies four types of prediction key points, including basic physiological characteristics, ovarian reserve function, treatment intervention and pregnancy outcome correlation. When the total amount of key points exceeds a first threshold value, a dimensionality reduction improved feedforward neural network is constructed, and basic physiological characteristics and treatment intervention data are used as inputs to predict ovarian function and pregnancy outcome. When the number of key points is small, a graph neural network is used, a directed graph structure is constructed based on clustering and hierarchical relationship of each key point, and node state is dynamically updated based on basic physiological characteristics, so as to realize classification prediction of ovarian function and pregnancy risk, improve prediction accuracy and model interpretability, and provide personalized and intelligent decision support for fertility evaluation of POI patients.

The invention belongs to the technical field of assisted reproduction, and particularly relates to a biomarker combination and a method for evaluating the failure risk of embryo implantation and early pregnancy of in-vitro assisted pregnancy patients. According to the method, the risk of embryo implantation failure is evaluated by detecting the oxidative stress marker concentration of an in-vitro pregnancy assisting patient at a specific time point. The multivariate logistic regression analysis proves that the increase of MDA, non-heme, 12-HETE and 15-HETE in serum is an independent risk factor of embryo implantation failure in the period; wherein the elevation of MDA and non-iron heme is a risk factor of occurrence of repeated implantation failure (RIF); the increase of MDA in uterine cavity fluid is an independent risk factor for early pregnancy failure. The invention provides a risk assessment method, which is characterized in that the concentration of the corresponding marker in the specific sample is detected, and the risk of the patient is assessed according to logistic regression analysis. The method has the advantages that high-risk patients can be recognized before transplantation, individualized diagnosis and treatment are achieved, and the accumulative pregnancy rate is increased.

The invention discloses a high-quality embryo screening method based on embryo image and free RNA (cfRNA) multi-modal fusion, and belongs to the technical field of assisted reproduction. The method is based on a single embryo culture system and comprises the following steps: collecting a culture solution of a blastocyst-stage embryo and a corresponding embryo image and clinical information, obtaining molecular characteristics of cfRNA in the culture solution by adopting an ultramicro cfRNA sequencing library preparation technology, and extracting embryo morphology and time sequence dynamic characteristics in combination with a deep learning algorithm. And a multi-modal prediction model fusing molecule and image information is constructed, so that non-invasive accurate evaluation of embryo quality, pregnancy rate and live birth rate is realized. According to the method, the problems of high subjectivity, high invasive risk and insufficient prediction accuracy of traditional morphological evaluation are solved, the accuracy of high-quality embryo screening is improved through multi-modal information fusion, a safer and more efficient embryo evaluation scheme is provided for an assisted reproduction technology, and the method has important clinical application value.

The present application relates to the technical field of giant panda breeding, and discloses a method for judging the true or false pregnancy and the expected delivery date of a giant panda, which comprises the following steps: collecting and detecting the progesterone value of the urine sample of a pregnant giant panda every day after mating; counting the amount of excrement of the pregnant giant panda; taking the day when the progesterone value of the excrement of a giant panda reaches the peak as day 0, taking the number of days as the horizontal coordinate, and taking the progesterone value of the urine sample and the amount of excrement of the pregnant giant panda as the vertical coordinate, to construct a pattern diagram of the change of the progesterone value and the amount of excrement of the giant panda; and judging the true or false pregnancy and the expected delivery date of the giant panda by observing the pattern diagram. The true or false pregnancy of the giant panda can be judged by observing the decrease of food and the change of the amount of excrement of the giant panda after the C point, and the expected delivery date of the true pregnant giant panda can be predicted by the time after the progesterone value peak B point. Therefore, the management personnel can strengthen the management of the giant panda in the embryo implantation period, effectively prevent the early embryo loss of the giant panda, and improve the pregnancy rate of the giant panda and prevent the early abortion of the fetus.

The invention discloses an sgRNA (small guide ribonucleic acid) pair for constructing a low-fertility mouse model as well as a construction method and application of the sgRNA pair. The sgRNA pair consists of sgRNA1 (SEQ ID NO.1) and sgRNA2 (SEQ ID NO.2) of a targeted mouse 1700031 M16Rik gene. A CRISPR-Cas9 system formed by the sgRNA pair and Cas9 protein is used for performing gene editing on a mouse fertilized egg, and exons 1-11 of the 1700031 M16Rik gene can be efficiently knocked out, so that a stably inherited low-fertility mouse model is successfully constructed. The model has definite phenotypes such as remarkable reduction of pregnancy rate and litter number, increase of sperm malformation rate and the like, is good in specificity, provides an ideal experimental tool for researching a molecular mechanism of human low fertility and screening medicines, and has important scientific research and clinical values.

The application relates to a preparation method of an animal sperm liquid storage diluent. The preparation method of the animal sperm liquid storage diluent comprises the following steps: (1) mixing glucose, fructose, tris-hydroxymethyl aminomethane, citric acid and water to obtain a mixture 1; the mass-volume ratio of the glucose, the fructose, the tris-hydroxymethyl aminomethane, the citric acid and the water is (10-20) g:(10-20) g:(20-30) g:(10-20) g:(50-100) ml; (2) mixing edible multiple plant blended oil and the mixture 1 in the step (1), stirring to obtain a mixture 2; standing, discarding the upper oil, refrigerating, and diluting 10 times in volume with sterilized distilled water. The application has the advantages of wide application range, high pregnancy rate, long animal sperm liquid storage time, good storage activity and the like.

The invention provides a method for regulating and inducing conception of non-pregnant non-oestrus dairy cows. The method is characterized by comprising the following steps: 1) detecting LH and FSH levels and performing staging; 2) carrying out regulation and targeted induction on cows with different LH and FSH stage levels; and 3) artificial insemination. Targeted treatment and precise regulation and control are achieved for high-difficulty breeding cases of 37 sky huai, no oestrus, lasting corpus luteum and uterus residues. The prostaglandin PGF2a + oxytocin + gonadotropin releasing hormone GnRH combined intramuscular injection scheme is adopted, lasting corpus luteum is thoroughly dissolved, residual embryonic tissues in the body are removed, the uterus is purified, the subsequent conception environment quality is remarkably improved, ultrasonic monitoring does not need to be frequently used, individualized and refined hormone regulation is achieved, the conception rate is remarkably increased, and meanwhile the conception time is shortened. The one-birth double-calf rate is stably improved, and the breeding economic benefits are greatly improved.

The application provides a method for artificial insemination of sheep, which synchronously induces estrus of ewes by implanting a vaginal sponge, promotes follicular development by combined injection of pregnant mare serum when the sponge is removed, and ensures accurate mating time by combining estrus testing and timed insemination scheme; after training by supplemental feeding, semen is collected by using a false vagina with a temperature of 38-40 DEG C, and the motility of the diluted semen is maintained above 0.6; at least one of the two ways of conventional insemination or laparoscopic insemination is selected for insemination; the laparoscopic insemination establishes an operation channel by puncturing the base of the udder of the ewe, and the semen is accurately injected into the first third of the large bend of the uterine horn on both sides under the guidance of the laparoscope, so that the pregnancy rate can reach above 90%; and the pregnancy is checked by using B-ultrasound 25-30 days after mating. The method significantly improves the estrus concentration of ewes, the utilization rate of semen and the pregnancy rate, and is suitable for the reproductive management of large-scale and intensive sheep farms.

The application relates to application of an animal sperm liquid storage diluent. The animal sperm liquid storage diluent can be used for storing animal sperm; the animal sperm liquid storage diluent is prepared by the following method: (1) mixing glucose, fructose, tris-hydroxymethyl aminomethane, citric acid and water to obtain a mixture 1; the mass-volume ratio of the glucose, the fructose, the tris-hydroxymethyl aminomethane, the citric acid and the water is (10-20) g:(10-20) g:(20-30) g:(10-20) g:(50-100) ml; (2) mixing edible mixed plant oil and the mixture 1 of the step (1), stirring, the volume ratio of the edible mixed plant oil and the water is 50:(50-100), to obtain a mixture 2; standing, discarding the upper oil, refrigerating, diluting with sterilized distilled water. The application has the advantages of wide application range, high pregnancy rate, long animal sperm liquid storage time, good storage activity and the like.

The invention relates to a piston type embryo transfer tube, which comprises a lead-in tube part, a catheter part and a push-in part. The right end of the lead-in tube part can be pre-bent to a certain angle. The catheter part contains a catheter and a supporting tube sleeved on the left side of the catheter, and the right end of the catheter extends outwards relative to the supporting tube. The push-in part comprises a push rod, a piston body and a handle end respectively arranged at the two ends of the push rod. The piston body extends into the catheter cavity of the catheter, and the outer wall of the piston body is in contact with the inner wall of the catheter cavity. The piston body can be pushed by the push rod to approach the right end port of the catheter cavity. The push rod is formed with an elastic deformation section, so that the piston body can be bent relative to the push rod. The patent can ensure that the embryo is fully pushed into the uterus during embryo transfer, and it is not necessary to check whether the embryo is retained in the catheter in subsequent inspection. The time of the lead-in tube staying in the uterus can be shortened, which helps to reduce the stimulation of the uterus during embryo transfer and helps to improve the pregnancy rate.

This invention provides an sgRNA sequence targeting the MSTN gene in Rongchang pigs and its application, relating to the field of biotechnology. The nucleotide sequence of the target sequence in the sgRNA sequence is shown in any of SEQ ID NO. 1-3. This invention precisely edits a portion of exon 1 of the Rongchang pig MSTN gene using CRISPR / Cas9 technology, achieving for the first time targeted regulation of this gene's function, laying a crucial foundation for the breeding of high-quality Rongchang pigs. This invention only requires editing a 1-14 bp sequence to achieve frameshift mutations and other inactivating proteins in the MSTN gene. The targeting efficiency of the three sgRNAs designed in this invention all exceed 70%, especially sgRNA-2, which has a targeting efficiency as high as 84.6%. More importantly, in pregnancy experiments on sows after embryo transfer, the pregnancy rate reached 75%, and comparative studies of weight changes during the growth process of MSTN knockout pigs confirmed a positive impact on pig growth performance, moving beyond the level of laboratory cell gene editing and providing a reliable guarantee for practical industrial applications.

The invention provides a method for improving the transfer developmental capacity of a frozen bovine IVF (in-vitro fertilization) embryo, which is characterized in that in the processes of freezing and unfreezing the bovine IVF embryo, culturing the embryo in a later period and the like, high-concentration sodium pyruvate, insulin, TLR2 (toll-like receptor 2) and a Cytosporone B are independently or jointly added, so that the survival rate and developmental capacity of the frozen embryo, the pregnancy rate after transplantation and the like are improved. Experiments show that the embryo survival rate, the hatching rate and the embryo transfer pregnancy rate of a group which is jointly added with sodium pyruvate, insulin, TLR2 and Nur77 agonist B are obviously higher than those of a double-substance adding group, a single-substance adding group and a control group. The method lays a foundation for production and related scientific research of in-vitro fertilized embryos in the future, provides an effective, safe and feasible strategy for improving in-vitro embryo production efficiency and embryo quality, and has great popularization value.

The invention provides a special feed for cows and a preparation method thereof, and belongs to the technical field of cattle breeding nutrition, the special feed for cows comprises the following raw materials: corn, soybean meal, cottonseed meal, wheat bran, compound inclusion selenium yeast, a motherwort extract, vitamin E, sodium bicarbonate, bacillus subtilis, saccharomyces cerevisiae and a mineral premix; the special feed for cows provided by the invention can realize efficient absorption of selenium, accelerate involution of uterus, improve the oestrus concentration rate and conception rate, effectively reduce the occurrence rate of retained fetal membranes and postpartum infection, improve the double-birth rate, comprehensively improve the breeding efficiency and health level of cows, and reduce the comprehensive breeding cost.

The invention provides a regulation and control method based on correlation of porcine reproductive endocrine rhythm of intestinal microorganisms, which comprises the following steps: firstly, collecting pig manure and blood samples, detecting the structure and diversity of the intestinal microorganism community by using high-throughput sequencing, and detecting reproductive endocrine indexes such as follicle stimulating hormone and the like by using an ELISA (Enzyme-Linked Immunosorbent Assay) method. Then, through correlation analysis, establishing a correlation model of the enteric microorganisms and the reproductive endocrine rhythm, and finding out key enteric microorganisms; then, according to an analysis result, a targeted regulation and control scheme is formulated from the aspects of diet, microbial transplantation, environment and the like. And finally, regularly monitoring the regulation and control effect and adjusting the scheme. The method can realize accurate regulation and control, avoids the problem of drug residue in traditional hormone regulation, and is green and safe. The embodiment shows that the pig intestinal microflora can be optimized, the reproduction endocrine rhythm can be improved, the oestrus rate and the pregnancy rate can be remarkably increased, the early oestrus period can be advanced, and considerable economic benefits can be brought to the pig industry.