84 results about "Pregnancy rate" patented technology

The invention discloses a biological composite material as well as a preparation method and application thereof. The biological composite material comprises a decellularized biological amnion, mesenchymal stem cells and polypeptide hydrogel. In the polypeptide hydrogel, the sequence of the polypeptide is IGSRVGDRGDS. The biological composite material can effectively promote cell growth at a thin endometrial injury position, endometrial cell proliferation activity is higher, new vessel production related gene regulation activity is enhanced, fibrosis caused by the endometrial injury is well inhibited, re-adhesion after an intrauterine adhesion separation operation is prevented, and a good application prospect is achieved. The fertility is obviously improved, and the pregnancy rate is increased.

The invention discloses a traditional Chinese medicine compound oral preparation for treating male oligoasthenospermia, and belongs to the technical field of traditional Chinese medicine preparations. The rhizoma polygonati processed with wine, the prepared rehmannia root and the like are used for nourishing the liver and kidney to consolidate congenital, the codonopsis pilosula, the astragalus membranaceus and the like are used for invigorating spleen and replenishing qi to complement postnatal, the fried cowherb seed is used for activating blood circulation, and the salted semen plantaginis is used for promoting diuresis, so that the problems of insufficient spermatogenesis and low vitality caused by deficiency of the spleen and the kidney are fundamentally solved. Experiments and clinical researches show that the preparation can significantly improve the sperm quantity, activity and activity rate of oligoasthenospermia model mice and patients, the clinical total effective rate reaches 90%, the pregnancy rate reaches 53.3%, and the curative effect is superior to that of Wuzi Yanzong pills in a control group. The action mechanism covers regulation of blood lipid metabolism, improvement of hypothalamus-pituitary-gonad axis functions, repair of testicular seminiferous tubule structural damage and reduction of lipid deposition, and meanwhile, oxidative stress and inflammatory injury are relieved and spermatogenic cell functions are protected by regulating and controlling a PPAR signal channel and inhibiting molecular pathways such as Cga / Sting mediated cell apoptosis.

The invention relates to the technical field of panda breeding, and discloses a panda expected date of confinement and true and false pregnancy judgment method which comprises the following steps: after panda mating, collecting and detecting the progesterone value of a urine sample in a gestation period every day; counting the excrement amount of the giant pandas in the gestation period; taking the current day of the progesterone peak value of the farrowing panda as 0 day, taking the number of days as an abscissa, and taking the progesterone value and the excrement amount of a urine sample in the gestation period of the panda as an ordinate to construct a change mode pattern of the progesterone value and the excrement amount of the panda; and the true and false pregnancy conditions and the expected date of delivery of the panda are judged by observing the change mode pattern. According to the method, the true and false pregnancy conditions of the pandas can be judged by observing the food reduction and excrement amount change conditions of the pandas after the point C, and the expected date of birth of the true pregnancy pandas is predicted according to the time after the progesterone value peak point B. Therefore, management personnel can conveniently strengthen gestation period management on the pandas in the embryo implantation period; the method effectively prevents early embryo loss of pandas, improves the pregnancy rate of pandas, and prevents early abortion of fetuses.

The invention relates to the technical field of medicine application, and discloses a medicine composition for preventing or treating reproductive dysfunction of elderly women and application of the medicine composition. The invention provides a new therapeutic drug and means for prevention and treatment of reproductive dysfunction of elderly women. By constructing an animal model of reproductive dysfunction of the elderly women and taking the ovulation number of animals, hormone level related to reproduction, pregnancy rate and progeny development condition as detection indexes, the pharmacodynamic effect and safety of the pharmaceutical composition and the influence of the pharmaceutical composition on progeny development quality and genetic variability are evaluated for the first time; the effectiveness and safety of the pharmaceutical composition for treating reproductive dysfunction of elderly women are fully proved.

The invention relates to the technical field of animal husbandry, in particular to a synchronous oestrus-timing insemination method suitable for cattle, which comprises the following steps: firstly, performing intramuscular injection of PG (cloprostenol sodium) at different time points after calving to promote uterine recovery and luteolysis and lay a synchronous oestrus foundation; the method comprises the key steps that GnRH (gonadotropin release hormone) is given through intramuscular injection on the 43rd day, the 53rd day, the 60th day and the 69th day, pituitary is promoted to release follicle-stimulating hormone (FSH) and luteinizing hormone (LH), collection and screening of follicles are guided, follicle maturation is promoted, the oestrus uncovering rate and the oestrus conception rate are increased, a melatonin implant is implanted in a subcutaneous implantation mode on the 63rd day, and the melatonin implant is implanted in a subcutaneous implantation mode on the 63rd day. The synthesis of ovarian hormones is promoted by regulating the hypothalamus-pituitary-gonad axis, the secretion of follicle cell factors and maturation promoting factors is promoted, the development of granular cells is promoted, and the maturation of oocytes is promoted. According to the method, the pregnancy rate of a double-synchronization-timing insemination scheme which is most widely used in a large-scale dairy cow breeding pasture at present can be remarkably increased, and feasible technical support is provided for success of increasing the breeding efficiency of cows.

The invention discloses a timed insemination method for improving the pregnancy rate and the lambing rate of sheep, and belongs to the technical field of livestock breeding. In order to solve the problem that the pregnancy rate of existing sheep is unstable during regular insemination, the method comprises the steps that a progesterone sponge suppository is embedded in the vagina of a healthy and reproductive ewe on the 0th day, and meanwhile 25 g of luteinizing hormone is injected in an intramuscular mode to release hormone A3; removing the thrombus on the 12th day, and performing intramuscular injection of 400IU of pregnant mare serum gonadotropin at the same time; and after the suppository is removed, insemination is carried out on the cervix orifice of the ewe with ram semen diluted at the ratio of 1: (5-8) in 52h and 64h respectively. According to the method, ovarian activities of the ewes are regulated and controlled, the estrus synchronism and the ovulation rate are improved, tests show that the pregnancy rate reaches 81.6%, the lambing rate is 1.14, compared with a conventional method, the pregnancy rate and the lambing rate of the sheep are remarkably improved, and development of the sheep industry is promoted.

The invention provides a goat semen cryopreservation solution. The goat semen cryopreservation solution comprises melatonin and one or more of sodium citrate, fructose, yolk, glycerol, penicillin, streptomycin and tris (hydroxymethyl) aminomethane. The cryopreservation liquid is used for preserving goat semen and can improve the pregnancy rate of long-term infertility ewes.

The invention discloses an application of a secretory curl-associated protein 2 gene as a new target for treating uterine scars, the secretory curl-associated protein 2 gene (sFRP2) promotes the progress of the uterine scars, interferes, silences and inhibits the uterine scars, and the sFRP2 is knocked out, so that the occurrence and development of the scar uterus can be relieved, the pregnancy rate of women can be increased, and the adverse pregnancy outcome can be reduced. In order to further improve the curative effect that sFRP2 gene expression is inhibited by sFRP2-targeted small interfering RNA (issFRP2) so as to inhibit uterine scar formation, ionizable lipid, distearoyl phosphatidylcholine (DSPC), cholesterol and 1, 2-dimyristoyl-rac-glycerol-3-methoxy polyethylene glycol (DMG-PEG) are mixed and dissolved in ethanol according to a certain molar ratio, issFRP2 is dissolved in a citric acid buffer solution, and the issFRP2-targeted small interfering RNA (issFRP2) is added into the citric acid buffer solution so as to obtain a mixed solution; an ethanol phase and a water phase are mixed through a microfluid device, and the mixed solution is dialyzed in a PBS solution for two hours to prepare lipid nanoparticles. And then mixing the polycarbophil, the poloxamer and the lipid nanoparticles to prepare the composite hydrogel. The hydrogel can locally release sisFRP2 in the uterus, inhibit fibrosis, reduce collagen deposition and repair uterine scars. The hydrogel is good in biocompatibility and noninvasive, can realize slow release of the medicine, avoids repeated administration, has no obvious side effect on a maternal body and a fetus, and can also be used for other fibrosis-related diseases.

The present invention relates to the field of animal husbandry and veterinary technology, and specifically to a combined preparation for improving the conception rate of bovine embryo transplantation, and a preparation method and application thereof. The combined preparation comprises preparation A and preparation B; the active ingredients of preparation A are sodium selenite and vitamin E; the active ingredients of preparation B are chorionic gonadotropin and luteinizing hormone releasing hormone A3. By intramuscularly injecting preparation A with active ingredients of sodium selenite and vitamin E into the recipient bovine for embryo transplantation at 8 and 11 months of age, and intramuscularly injecting preparation B with active ingredients of chorionic gonadotropin and luteinizing hormone releasing hormone A3 into the recipient bovine for embryo transplantation on the day of embryo transplantation, the conception rate of bovine embryo transplantation can be improved. When preparation A and preparation B are used together at the same time, the transplantation conception rate can be significantly improved.

The invention relates to the technical field of medical instruments, in particular to a surgical device for artificial insemination in reproductive medicine, which comprises a syringe and a support, a control panel and a hose fixed at the top of the syringe are fixedly connected to the outside of the support, and semen injection holes are uniformly formed in the top end of the hose; the suction assembly is fixedly mounted on the syringe and is used for sucking semen; the supporting assembly is movably arranged outside the hose and used for assisting the hose in entering the vagina; according to the scheme, through the fixed connection mode of the hose and the syringe, the operation steps are simplified, through mutual cooperation of the first piston, the first connecting rod, the filter membrane and other structures, seminal fluid can be adsorbed into the syringe through the suction assembly, then seminal fluid outflow caused by loosening of a connector can be avoided, the operation success rate is further increased, and the pregnancy rate of a patient is further guaranteed.

The invention discloses a novel embryo transplant gun for cattle which can bend and extend forward along with the uterine horn, and relates to the field of embryo transplant guns. The invention comprises a gun barrel, a gun core and a culture fluid capillary, wherein the culture fluid capillary is matched and installed in a barrel hole at the inner end of the gun barrel, a piston is arranged in the culture fluid capillary, the gun core is arranged in the gun barrel, and the gun barrel also comprises an inflatable part, a hollow steel needle and an extension catheter, wherein the extension catheter is a hose with a hollow interior. The invention pushes the embryo in the capillary into the extension catheter through the piston, and then pierces the piston through the hollow steel needle to communicate with the inner cavity of the capillary, an operator pushes the piston handle of the syringe to allow the gas in the syringe to enter the extension catheter through the hollow steel needle, and relies on the gas to inject the embryo in the extension catheter into the uterine horn of the cattle, thereby solving the problem that the gun core cannot inject the embryo into the uterus after the capillary is connected to the extension catheter, and the operator does not need to grab the front end of the uterine horn, thereby greatly reducing the operating difficulty, facilitating the use of the operator, and indirectly improving the conception rate.

The application discloses an antioxidant combined diluent for improving the freezing preservation effect of sheep semen and application thereof. The mitochondrion-targeted antioxidant SS-31 is combined with the plant-derived antioxidant CUR for the freezing preservation of sheep semen, so that the generation and action of peroxides can be inhibited, the antioxidant capacity of sperm is enhanced, oxidative stress damage can be effectively avoided, the quality of frozen sheep semen is improved, the fertilization capacity is maintained or improved, and the pregnancy rate is improved.

The invention discloses a traditional Chinese medicine composition for treating PCOS pregnancy loss and application of the traditional Chinese medicine composition. The traditional Chinese medicine composition comprises the following raw material medicines in parts by weight: 20-40 parts of salted semen cuscutae, 20-40 parts of prepared rehmannia root, 10-20 parts of scutellaria baicalensis, 10-20 parts of dogwood, 20-30 parts of astragalus membranaceus, 20-30 parts of bighead atractylodes rhizome, 20-30 parts of white paeony root, 10-20 parts of parasitic loranthus and 10-20 parts of teasel According to the invention, active component groups (mass spectrum identification) and accurate compatibility design (kidney tonifying and miscarriage prevention theory) are defined, so that (1) the PCOS pregnancy rate is obviously increased (52%-80%, experimental verification); (2) synchronously improving reproduction-metabolism-uterus / placenta triple defects (data support such as a < HOMA-IR <->, a < Total T <->, a < Hif1 alpha > <-> and the like); and (3) the blank in the prior art is filled, and an integrated treatment scheme with both embryo protection and placenta function repair is provided. Experimental data, a molecular mechanism and traditional Chinese medicine theories prove the innovativeness and clinical value of the traditional Chinese medicine composition.

The present invention is applicable to the technical field of feed additives, and provides a feed additive and application thereof for improving the reproductive performance of ewes, wherein the feed additive includes D-aspartic acid and N-carbamylglutamate, and the masses of D-aspartic acid and N-carbamylglutamate are 0.5 grams and 1.5 grams, respectively. The present invention innovatively combines D-aspartic acid with N-carbamylglutamate for ewe breeding, which can significantly improve the fertility of ewes and is suitable for promotion in farms. The feed additive can effectively improve the number of superovulations, high-quality egg rate, estrus rate, pregnancy rate, and number of lambs of ewes, while improving the health of lambs. Compared with reproductive hormones, functional amino acids have low cost, simple operation, no increase in labor burden, and overcome the problem of side effects such as abnormal ovarian development caused by long-term use of reproductive hormones to improve the fertility of ewes, ensuring the green, healthy and sustainable nature of the breeding process, and laying a solid foundation for the long-term development of the breeding industry.

The invention relates to the technical field of medicine, and particularly discloses an endometriosis prescription for dysmenorrhea with endometriosis. Comprising the following raw materials: 10-15 parts of radix salviae miltiorrhizae, 7-10 parts of rhizoma curcumae, 2-3 parts of flos carthami, 7-10 parts of vinegar turtle shell, 10-15 parts of salted radix achyranthis bidentatae, 7-10 parts of rhizoma corydalis, 10-15 parts of prepared fingered citron, 5-8 parts of radix bupleuri, 10-15 parts of radix paeoniae alba, 10-15 parts of caulis spatholobi, 10-15 parts of poria cocos, 7-10 parts of herba taxilli, 7-10 parts of radix dipsaci, 3-5 parts of fructus amomi, 7-10 parts of endothelium corneum gigeriae galli, 10-15 parts of hawthorn and 7-10 parts of liquorice. The agreement prescription of the internal different prescription has obvious curative effects on improvement of dysmenorrhea symptoms of EMs patients, reduction of tentacle nodules and appendage masses, improvement of pelvic cavity states, increase of pregnancy rates and the like, and is safe and free of obvious toxic and side effects.

The invention discloses a traditional Chinese medicine composition for treating insufficiency of kidney yang and a preparation method thereof, and relates to the technical field of traditional Chinese medicines. The traditional Chinese medicine composition is prepared from the following components in parts by weight: 20 to 35 parts of rhizoma polygonati extract, 20 to 35 parts of semen astragali complanati, 20 to 35 parts of herba epimedii, 10 to 15 parts of rhizoma drynariae, 10 to 15 parts of fructus rubi, 10 to 15 parts of herba verbenae, 5 to 15 parts of rhizoma polygonati, 5 to 15 parts of cortex eucommiae and 5 to 15 parts of fructus psoraleae. Through scientific compatibility and an optimized preparation process, the curative effect of the traditional Chinese medicine composition in the aspect of treating kidney-yang deficiency is remarkably improved. Experimental results show that the traditional Chinese medicine composition can obviously improve the kidney-yang deficiency symptom, increase the sperm quantity and activity, reduce the sperm malformation rate and improve the sexual organ weight and the female mouse pregnancy rate. In addition, the traditional Chinese medicine composition can be used for preparing traditional Chinese medicine preparations of various dosage forms, and has wide application prospects.

The application discloses a mesenchymal stem cell preparation and application thereof in thin endometrium repair, and comprises the following steps: adding Enterococcus durans CGMCC 1.3249 and Bacillus subtilis CGMCC 1.15792 supernatant as effective components into cultured mesenchymal stem cells; and the stem cell preparation obtained by the method can effectively restore the endometrial thickness of mice, increase the number of glands, and improve the pregnancy rate of the thin endometrium mice.

The invention relates to the technical field of bovine cloned embryo culture, and discloses a bovine cloned embryo culture solution and application thereof. The culture solution is prepared from water and comprises the following substances: 6.81 g / L of sodium chloride, 0.23 g / L of potassium chloride, 2.2 g / L of sodium bicarbonate, 0.04 g / L of sodium pyruvate, 0.76 g / L of calcium hemi-lactate, 0.05 v / v% of double antibodies, 2v / v% of essential amino acids, 1v / v% of non-essential amino acids, 0.146 g / L of glutamine, 4g / L of bovine serum albumin and 10-100 [mu] mol / L of NAD (nicotinamide adenine dinucleotide). The culture solution can obviously improve the blastocyst rate and pregnancy rate of the bovine cloned embryos and reduce the apoptosis rate and active oxygen level of the bovine cloned embryos, so that the quality of the bovine cloned embryos is obviously improved, a somatic cell cloning operation system of cattle is optimized, and the cost of the bovine cloned embryos from production to application is reduced.

The invention relates to the technical field of biological medicine, and particularly discloses a recombinant bovine interferon coated sustained release microsphere and a preparation method and application thereof, an ultrasonic re-emulsification method is used for replacing bare-handed re-emulsification, 2.5% of trehalose is added into an internal water phase W1, and the protein concentration of the internal water phase W1 is reduced by 30 mg / ml to 25 mg / ml. According to the method provided by the invention, the encapsulation efficiency reaches 90% or above, the recovery rate reaches 95%, the drug loading rate is close to 5%, the balling rate exceeds 90%, and the in-vitro effective slow release time reaches about 10 days; through clinical supplement of the coated slow-release rbIFNT, the pregnancy rate of the receptor cattle in the initial test and the re-test is obviously improved by about 10%, and the pregnancy rate of the repeat breeding infertility young cattle in the initial test is obviously improved by about 15%.

The invention discloses an embryo freezing resuscitation reagent and a resuscitation method, and belongs to the technical field of embryo freezing resuscitation. According to the embryo freezing resuscitation reagent provided by the invention, resuscitation liquid is improved, and compared with an existing 1M sucrose solution, the same resuscitation effect can be achieved only by adopting a 0.3-035 M sucrose solution. The reagent concentration is remarkably reduced, and the cost can be effectively reduced. The invention further provides a rapid resuscitation method matched with the embryo freezing resuscitation reagent, the whole resuscitation process of one embryo is about 2 min, 20 FET periods can be completed only about 40 min, the working efficiency is greatly improved, the operation process is simplified, the waiting time of a patient is shortened, and the satisfaction degree of the patient is improved. The embodiment proves that the rapid resuscitation scheme has no influence on the survival of the blastocyst, the survival rate of the resuscitated blastocyst reaches 100%, the continuous pregnancy rate can be effectively increased, and the abortion rate is reduced.

The invention belongs to the technical field of animal breeding, and particularly relates to application of alpha-ketoglutaric acid or salt thereof in preparation of a sheep semen cryopreservation diluent. The invention discloses the application of alpha-ketoglutaric acid or salt thereof in the cryopreservation of the sheep semen for the first time, and the alpha-ketoglutaric acid or salt thereof can improve the semen quality of the frozen and unfrozen sheep semen and improve the fertilization ability of the frozen semen; such as sperm motility, sperm movement performance, sperm viability, sperm plasma membrane integrity rate, sperm acrosome integrity rate, sperm antioxidant capacity, sperm ATP level, sperm early apoptosis incidence rate, sperm in-vitro fertilized ovulation rate and / or artificial fertilization pregnancy rate, can be used for preparing the sheep sperm cryopreservation diluent.

The invention belongs to the technical field of traditional Chinese medicines, and particularly relates to a traditional Chinese medicine composition for treating polycystic ovarian syndrome as well as a preparation method and application thereof. The traditional Chinese medicine composition is prepared from Chinese yam, semen cuscutae, pulp of dogwood fruit, radix glehniae, radix scrophulariae, lily, rhizoma polygonati, tortoise shell, cistanche deserticola, lotus seed and raspberry, has the effects of tonifying kidney and replenishing essence, nourishing yin and blood, replenishing qi and tonifying spleen, and benefiting uterus and accommodating fetus, and is mainly used for treating the polycystic ovarian syndrome. Research results show that the traditional Chinese medicine composition can significantly improve the sex hormone level of a patient with the polycystic ovarian syndrome, increase the endometrial thickness, improve the ovarian function and improve the endometrial receptivity, the ovulation rate and the pregnancy rate. Therefore, the traditional Chinese medicine composition has important clinical significance and wide market application prospects.

The present invention discloses a composition, method and application thereof for improving the quality of in vitro maturation of eggs and the pregnancy rate of embryos after in vitro fertilization. The present invention aims at the problems of low in vitro maturation rate and poor subsequent embryo development potential caused by metabolic disorders during in vitro maturation of oocytes, and provides a composition including two or more regulators in vitamin C, L-carnitine and thrombopoietin to regulate the metabolic mode of oocytes, thereby greatly improving the in vitro maturation rate of oocytes and the subsequent embryo development potential. The present invention not only improves the efficiency of in vitro embryo production of livestock, but also provides a reference for improving the quality of in vitro maturation of human assisted reproductive eggs. The method is simple, safe and efficient, and has great application value and broad application prospects.

The invention is suitable for the technical field of camellia oil application, and provides application of camellia oil in prevention and treatment of ovarian function decline. The invention reveals that the camellia oil has good prevention and improvement effects on the ovarian function decline for the first time. A series of rigorous scientific experiments prove that the camellia oil can effectively improve weight loss and reproductive hormone disorder caused by mouse ovarian function decline induced by cyclophosphamide, repair ovarian tissue damage and inhibit ovarian granular cell apoptosis, so that normal development of ovaries is promoted, the ovarian index is increased, and the effect of preventing and treating ovarian diseases is achieved. The number of atresia follicles is reduced, the pregnancy rate and the litter size are increased, and the occurrence of ovarian function decline is effectively prevented and improved. The discovery provides a scientific basis for preventing and improving ovarian function decline by using the camellia oil, and avoids tissue hyperplasia and canceration risks caused by strong stimulation of an endocrine system, reproductive organs and mammary gland by exogenous hormones in a hormone therapy.

The present invention discloses an additive for freezing liquid in a one-step method for bovine in vitro embryos and a one-step freezing method for bovine in vitro embryos, and belongs to the technical field of animal embryo cryopreservation and embryo transplantation. The additive of the present invention is an aqueous solution comprising sodium chloride, potassium chloride, magnesium chloride, disodium hydrogen phosphate, potassium dihydrogen phosphate, glucose, sodium pyruvate, calcium chloride, bovine serum albumin, sucrose, ethylene glycol and hyaluronic acid; the concentration of sucrose is 4.5 to 5.0 M. Based on the one-step freezing method for bovine in vitro embryos, the present invention increases the sucrose concentration in the conventional one-step freezing liquid and additionally adds hyaluronic acid, thereby increasing the osmotic pressure of the freezing liquid, facilitating the discharge of water inside the embryo, reducing the formation of ice crystals inside the embryo during the freezing process, reducing the damage to the embryo during the freezing process, and improving the survival rate, hatching rate and transplantation pregnancy rate of bovine in vitro embryos after freezing and thawing.

The invention relates to a freezing carrier device capable of absorbing embryo freezing fluid and application of the freezing carrier device. The freezing carrier device comprises a freezing pipe body, fluid-absorbing resin particles are adhered to the inner wall of the freezing pipe body through a polyurethane adhesive, tiny gaps exist between the fluid-absorbing resin particles, numerous micro-channels are formed, and a capillary effect is generated on the fluid. The liquid-absorbing resin particles used for absorbing the liquid can be polyacrylamide, sodium polyacrylate or a mixture of the polyacrylamide and the sodium polyacrylate. When the freezing carrier device is used, an embryo and a protective agent are mixed and then put into the freezing pipe body, and due to the liquid absorption performance (the hydrophilic effect of resin and the capillary effect of particle gaps) of the liquid absorption resin particle layer, the liquid absorption resin particle layer can rapidly absorb redundant embryo freezing liquid in embryo freezing liquid, so that the volume of the embryo freezing liquid is reduced, and the embryo freezing effect is improved. The freezing and unfreezing speed is increased, the damage of ice crystals to embryos is reduced, and the survival rate and conception rate of the embryos are increased.

The invention discloses application of resveratrol in preparation of a product for preventing and / or treating unexplained recurrent abortion, and belongs to the technical field of biological medicine. A small dose of resveratrol is subjected to intragastric administration treatment on a mouse with unknown recurrent abortion, so that the resveratrol not only reduces the embryo absorption rate, but also improves the inflammatory response of the decidua tissue of the mouse, improves the Sirt1 level of the decidua tissue of the mouse, and prevents the generation and development of the sterile inflammation of the maternal-fetal interface of the mouse with unknown recurrent abortion; further, the pregnancy rate of the mice with unexplained recurrent abortion is effectively protected and treated. The invention provides a new way and method for treating recurrent abortion caused by unknown reasons.

The application discloses high-adhesion modified mesenchymal stem cells and application thereof in preparation of a medicine for treating intrauterine adhesion. The modified mesenchymal stem cells are genetically engineered to overexpress integrin alpha 2 subunit and / or integrin alpha 5 subunit, thereby enhancing the adhesion capacity to the extracellular matrix (collagen and fibronectin) of the endometrium. In vitro experiments show that the initial adhesion rate, liquid flushing resistance and anoikis resistance of the modified MSCs are significantly better than those of common MSCs. When used in combination with the "dynamic culture and drug delivery device for intrauterine adhesion treatment" applied by the inventor on the same day, the retention rate of the modified MSCs in the simulated intrauterine dynamic culture environment is significantly improved. Animal experiments show that the modified MSCs in combination with the device can significantly promote endometrial regeneration, inhibit fibrosis and improve the pregnancy rate. The application solves the core bottleneck of low cell retention rate and short survival time in the MSC transplantation treatment of intrauterine adhesion by enhancing the adhesion capacity of the MSCs, and forms a complete treatment scheme in cooperation with the device, thereby having a good clinical application prospect.

The sperm screening device is sequentially provided with a sperm sampling component, a first filtering component, a sperm screening component, a second filtering component and a sperm collecting component from bottom to top, a sperm storage cavity is formed in the top surface of the sperm sampling part, the sperm screening part is provided with a vertically-through mucus storage cavity, and the sperm collecting part is provided with a vertically-through culture medium storage cavity; the first filtering part is provided with a plurality of first through holes penetrating up and down, and the two ends of the first filtering part are in contact with the sperm storage cavity and the mucus storage cavity respectively; the second filtering part is provided with a plurality of second through holes penetrating up and down, and the two ends of the second filtering part are in contact with the mucus storage cavity and the sperm collecting part respectively. The invention further provides a screening method using the sperm screening device. According to the sperm screening device and the using method thereof, sperms with the ultralow DNA fragmentation rate can be screened out, and the pregnancy rate and the offspring quality can be improved.

The invention provides a method for improving the timed insemination efficiency of cows, which comprises the following steps: injecting GnRH for the first time on the nth (n is 33-39) day after delivery of cows, and embedding CIDR at the same time; on the (n + 7) th day, PG is injected for the first time, and CIDR is withdrawn at the same time; injecting GnRH for the second time on the (n + 7 + 3) th day; injecting GnRH for the third time on the (n + 7 + 3 + 7) th day; injecting PG again on the (n + 7 + 3 + 7 + 5) th day; after PG is injected again for 56 h, GnRH injection is conducted for the fourth time; after 14-16 h of the fourth GnRH injection, artificial semen deposition is carried out; and carrying out fifth GnRH injection on the seventh day after artificial insemination, and respectively injecting ketoprofen on the fifteenth night and the sixteenth morning after artificial insemination. According to the method, the timing insemination pregnancy rate of the dairy cow can be remarkably increased, the timing insemination pregnancy rate of the dairy cow under the heat stress condition can also be increased, and the method has important economic value and social value for guaranteeing healthy development of dairy cow breeding enterprises.