35 results about "Gestation period" patented technology

The invention discloses a preparation method of a medicinal and edible traditional Chinese medicine composition for preventing and treating pregnancy complications. The medicinal and edible traditional Chinese medicine composition is prepared from the following raw materials in parts by weight: 24 parts of radix rehmanniae, 12 parts of fructus gardeniae and 9 parts of folium mori. The composition embodies the essence of compatibility of monarch, minister, assistant and guide of a traditional Chinese medicine compound, the radix rehmanniae is used as a monarch drug, the fructus gardeniae is used as a minister drug, the folium mori is used as an assistant drug, and the composition is mainly characterized by nourishing the kidney, containing wood, clearing away fire, calming the liver and lightly dredging upper jiao and exactly conforms to the core pathogenesis of yin deficiency and yang hyperactivity of gestational hypertension. Modern pharmacological experiments prove that the three traditional Chinese medicinal materials all have definite antihypertensive activity, and the action mechanism shows the characteristic of multi-target synergism. The raw materials in the formula are homologous in medicine and food, free of toxic and side effects, mild in medicine property and capable of considering fetal elements, the three medicines all belong to clinical common miscarriage prevention or miscarriage prevention categories, worries about blood breaking and fetal movement are avoided, and the traditional Chinese medicine is suitable for being used in the pregnancy stage. The pharmaceutical composition disclosed by the invention can be used for relieving clinical symptoms of hypertensive patients in pregnancy and improving the outcome of bad pregnancy.

Embodiments of the invention provide a solution for ameliorating microbiome effects on neurodevelopment by developing a probiotic composition for consumption by a female during gestation. In certain aspects the probiotic is a multi-strain probiotic cocktail consisting of immunomodulatory taxa and administered females during pregnancy and lactation.

The invention discloses a CHRM3 gene InDel molecular marker associated with growth and reproduction traits of a large white pig and application of the CHRM3 gene InDel molecular marker, a 62bp deletion variation is identified in a second intron of the CHRM3 gene, and a breakpoint is positioned in a second intron 521426525142713bp (a pig chromosome 14 reference sequence NC010456.5) of ENSSSCT00000082112.1. The application for detecting / identifying the growth and reproduction traits of the large white pigs is developed on the basis of the molecular marker, and the detection method is simple, rapid and low in cost and can be used for accurately screening individuals with better traits such as backfat thickness, primary birth litter weight, total multiparous litter size and gestation period of multiparous sows in the early stage, so that genetic improvement is carried out on the growth and reproduction traits of the pigs, and the economic benefit is increased. Multiple characters such as growth and reproduction can be selected at the same time, the breeding period of excellent boars can be shortened, and the breeding efficiency and progress are accelerated.

The invention relates to a method for detecting early embryo death of cattle and sheep, which is characterized by comprising the following steps of: 1) determining the detection time of early embryo death: when oestrus behavior characteristics of cows and ewes are observed by a pedometer or naked eyes, determining the time as the detection time; 2) detecting early embryo death: taking 5-10ml of cow and ewe blood, standing for 1-2 hours in a shade place, separating serum, respectively inserting bovine trophoderm protein-1 detection test paper and sheep trophoderm protein-1 detection test paper into the cow and ewe serum for 5-10 minutes, observing the detection test paper 15-18 minutes after the detection test paper is taken out, and when the detection test paper develops color, detecting the early embryo death; the cows or ewes are successfully fertilized and form early embryos, but still have estrus behavior characteristics, and the embryos are proved to be dead. Whether embryos die or not can be detected in time, the problems that the feeding cost is increased in the non-pregnant period, the number of fertilization opportunities caused by blind mating is small, and wombs of cattle and sheep are injured are solved, and by reasonably waiting for mating, the breeding efficiency is greatly improved, the yield of milk and goat milk is increased, and the income of a feeding farm is increased.

The present invention relates to the medical and food use of bile acid derivatives. Specifically, the present invention relates to the use of 3-oxoLCA in reducing oxidative stress and inflammation in pregnant mothers and their offspring. Specifically, the present invention relates to the use of 3-oxoLCA to reverse the effects of oxidative stress on pregnant mothers, in particular, to maintain fetal equal weight, significantly reduce placental and liver oxidative stress in pregnant mothers and the resulting inflammation or dysfunction of the placenta and liver, and to improve the intestinal flora imbalance of pregnant mothers. Further studies have found that 3-oxoLCA has long-term effects, in particular, can significantly reduce the effects of oxidative stress during pregnancy on oxidative stress and inflammation in the liver of offspring.

The invention belongs to the technical field of medicines, and particularly relates to application of harpagide in preparation of a medicine for treating constipation. The technical problem to be solved by the invention is to provide a safer, milder and new effective choice for treating and relieving constipation. The technical scheme for solving the technical problem is to provide application of harpagide in preparation of drugs or health care products for treating or relieving constipation. The harpagide achieves or even exceeds the treatment effect of the fluid-increasing decoction compound. Meanwhile, in the small pigs, the constipation symptom of the small pigs in the gestation period is also obviously relieved. When the harpagide is used as a medicine for treating constipation, a safer, more efficient and standardized treatment effect can be achieved, a safer and milder treatment choice is provided for patients with constipation, and the harpagide has a very good application prospect.

The invention discloses a culture medium for goat trophoblast organoid, a culture method and application, and belongs to the field of goat trophoblast organoid culture. The method comprises the following steps: separating trophoblast tissues from a healthy goat placenta which is pregnant for 80-90 days, digesting the trophoblast tissues through type II collagenase to obtain primary cells, compounding the primary cells with matrigel, and performing three-dimensional culture in an improved trophoblast organ culture medium with specific components to form mature organs. The organ is of a typical three-dimensional structure, and KRT23 and SOX2 markers are stably expressed. The organoid culture system based on the goat primary trophoblast cells is established for the first time, gene modification is not needed, the in-vivo physiological status is highly simulated, and the culture system is standardized and good in repeatability. The constructed organoid can be used as an ideal in-vitro model for researching placenta development, evaluating drugs and toxins and modeling infectious diseases in the gestation period, and is of great significance in improving the reproductive research level of goats and the production benefits of animal husbandry.

The invention is applicable to the technical field of biological medicines, provides application of ophiopogon japonicus borneol glycoside in preparation of a medicine for preventing and treating gestational metabolic diseases, and aims to solve the technical problems that the conventional medicine is limited in use during pregnancy, and the curative effect and the safety are difficult to take into account. According to verification of multi-model systems such as animals, cells and human-derived trophoblast organs, it is found that ophiopogon japonicus borneol glycoside can significantly improve gestational glycometabolism disorder, improve insulin sensitivity and reduce insulin resistance, and therefore effective intervention on gestational diabetes mellitus is achieved. As a natural component derived from the Zhejiang radix ophiopogonis, the radix ophiopogonis borneol glycoside has definite pharmacological activity and excellent safety, and the Zhejiang radix ophiopogonis is listed in a national homology of medicine and food, so that reliable safety guarantee is provided for use during pregnancy.

The invention discloses an application of an RNAm6A modification level of PTGER4 in preparation of an ICP (inductively coupled plasma) auxiliary diagnosis kit. The RNA m6A modification level of the PTGER4 in placenta tissue and whole blood of an ICP patient is remarkably reduced, and the AUC modified by the RNA m6A of the PTGER4 in the whole blood is 0.844, so that the RNA m6A modification of the PTGER4 is a biomarker highly related to ICP attack, and a laboratory support can be provided for screening, diagnosis and treatment of ICP.

The invention belongs to the technical field of livestock breeding, and particularly relates to a bidding system for breeding living bodies in an incubation period according to adult value, which comprises a livestock breeding period model, an adult livestock model, a bidding and bidding module and a customer query module, and is characterized in that the livestock breeding period model and the adult livestock model are used for evaluating the value of livestock during slaughtering; the evaluated value and various breeding data are uploaded to a bidding module for customers to look up, various bidding rules are sent out, and finally bidding is carried out through a customer query module. According to the invention, the data model is established during the livestock breeding period, the model is used to deduce and evaluate the value of the livestock marketing data, bidding is carried out on the basis of the value evaluation, pre-selling is completed during the breeding period, the original selling time is prolonged to the whole breeding period, the selling pressure is reduced, and the marketing efficiency is improved. Meanwhile, the slaughtered livestock is prevented from occupying the farm, the next batch of livestock can be bred after slaughtering, and the economic benefit of livestock breeding is increased.

The method for predicting the severe brain injury probability of the newborn is used for predicting the severe brain injury probability of a selective fetal growth limited double-fetal newborn, and the selective fetal growth limited double-fetal newborn comprises a to-be-detected large fetus and a to-be-detected small fetus. The method comprises the following steps: acquiring a meconium sample of a large fetus to be detected and a meconium sample of a small fetus to be detected; the method comprises the following steps: pretreating and detecting a meconium sample of a large fetus to be detected and a meconium sample of a small fetus to be detected to obtain the concentration of marker metabolites in the meconium sample to be detected; according to the concentration of the marker metabolite of the big fetus and the concentration of the marker metabolite of the small fetus, the probability of severe brain injury of the big fetus and the small fetus is predicted; wherein the big fetus marker metabolite is different from the small fetus marker metabolite; according to the embodiment of the invention, the characteristic of recording the intrauterine metabolism state of the fetus in the whole gestation period by virtue of meconium is utilized, and the severe brain injury risk can be predicted in the early stage after birth. Different specific markers are screened for fetuses of different sizes, a prediction model is established, and the prediction result is accurate.

The invention relates to medical and food use of bile acid derivatives. Specifically, the invention relates to an application of 3-oxoLCA in reducing oxidative stress and inflammation of a pregnant maternal and offspring of the pregnant maternal. Specifically, the invention relates to the use of 3-oxoLCA to reverse the influence of oxidative stress on the pregnant maternal, in particular, to maintain the average weight of a fetus, to significantly reduce the oxidative stress of the placenta and liver of the pregnant maternal and the inflammation or dysfunction of the placenta and liver caused thereby, and to improve the intestinal flora imbalance of the pregnant maternal. Further research finds that the 3-oxoLCA has long-term influence, and particularly, the 3-oxoLCA can obviously reduce the influence of oxidative stress in the gestation period on the oxidative stress and inflammation of offspring liver.

The invention discloses application of indoleacetic acid in diagnosis and treatment of gestational diabetes mellitus. Clinical queue research finds that the concentration of serum indoleacetic acid of pregnant women in a gestational diabetes mellitus group is remarkably lower than that of pregnant women in a healthy control group, and high diagnosis efficiency is achieved. Animal experiments further prove that in a high fat diet induced mouse model with gestational diabetes mellitus, the exogenous indoleacetic acid is supplemented, so that the fasting blood glucose of a female mouse with gestational diabetes mellitus can be reduced, abnormal glucose tolerance and insulin resistance can be improved, and the indoleacetic acid is free of embryotoxicity, safe and reliable. The application provides a new thought and means for early diagnosis and effective treatment of gestational diabetes mellitus, and has a wide application prospect.

A system, device, and method of controlling, supporting, and / or elongating the cervix uteri are provided to treat gestation complications and / or delay the onset of childbirth such that the gestation period of a fetus can be extended to prevent preterm birth and childbirth of the fetus. A cervical control system may include a body configured to be placed in the vagina. The body has an internal channel configured for receiving a cervix therein and an inflatable and / or inflatable inner cuff extending from a portion of the internal channel. The inner cuff may inflate inwardly toward the cervix, or downwardly away from the cervix, or both inwardly and downwardly, such that the inner cuff may engage or clamp the cervix to secure the cervix control system in place while pressing the cervix to close, elongate, and / or adjust the cervix orientation angle.

The invention discloses an SNP (Single Nucleotide Polymorphism) genetic marker associated with the number of days in the gestation period of sows and application of the SNP genetic marker, and belongs to the technical field of pig genetic marker detection, the SNP genetic marker is located at a 26188159 bp nucleotide site of a No.7 chromosome of a reference sequence of an international pig genome version 11.1, the basic group of the site is C or T, and the SNP genetic marker is located at the upstream 373200 bp position of an MLIP gene. The invention also discloses an application of the SNP genetic marker in breeding sows with short gestation period days. The SNP genetic marker can be used for screening sows with short gestation period days, provides a new marker resource for auxiliary selection of breeding sows, and can be used for genetic breeding of the breeding sows.

The application belongs to the field of pig marker-assisted selection technology, and particularly relates to application of a SNP molecular marker related to a pig gestation period trait. The SNP molecular marker is at a base at a 32922080 locus of a first chromosome in a pig genome Sscrofa11.1.109. The SNP locus of the molecular marker has C / G polymorphism, wherein C is a favorable allelic variation of the pig gestation period trait. The superior allele of the SNP can increase the frequency of the superior allele generation by generation, shorten the gestation period of the pig, and accelerate the genetic improvement progress of the pig, thereby effectively reducing the cost of breeding of a breeding pig.

The invention discloses an animal model construction method for fetal osteoporosis, a prevention and treatment target and application of the prevention and treatment target. The fetal osteoporosis animal model is a filial generation rat which is obtained after a pregnant female Wistar rat is subjected to intragastric administration of Acimoline (300 mg / kg. D) in the pregnancy period. Weaning is conducted four weeks after birth, male and female are separated from cages and fed to 12 weeks, the peak bone mass of female offspring is remarkably reduced, and therefore the animal model with exposed Acimoline during pregnancy is established. On the basis of a pregnancy-stage Acimoline exposed rat model, 20 mg / kg hippuric acid is intragastric injected in the pregnancy stage, and peak bone mass reduction of female offspring can be effectively corrected. The animal model established by the invention is novel, reliable, simple and convenient, and application of hippuric acid in screening and preparing medicines for preventing and treating fetal osteoporosis is determined.

The invention discloses application of icariin or icariin composition in preparation of a medicine for cross-generation improvement of fertility of male mammals, and belongs to the technical field of biological medicine. It is confirmed for the first time that when icariin is given to the parental generation in the gestation period, remarkable cross-generation protection and improvement effects can be generated on fertility of male offspring of the parental generation. The discovery surpasses the traditional cognitive framework that icariin is only used for treating contemporary individual sterility, and reveals a brand-new and proactive application value of icariin in the field of reproductive health. The invention provides a brand new active ingredient and an intervention strategy for preventing and treating cross-generation male reproduction injury caused by factors such as parental environment exposure and the like, and has important scientific value and clinical application prospect.

The invention belongs to the technical field of animal model construction, and particularly relates to a construction method and application of a preeclampsia animal model, and the construction method comprises the following steps: injecting CdCl2 into a gestational animal to construct the preeclampsia animal model. The construction method disclosed by the invention is high in success rate, low in abortion rate, relatively low in toxicity and high in safety, and can be used for long-term intervention, i.e., the change of the early pregnancy can be observed from the early pregnancy intervention, and N-nitro-L-arginine methyl ester used in the prior art is very high in toxicity, so that a very high abortion rate can be caused if the early pregnancy intervention is carried out. The construction method is low in cost, CdCl2 used for injection is low in unit price, economic benefits are high, and the construction method is easy to operate and convenient to popularize.

The invention discloses an application of plasma mtDNA in predicting congenital heart disease related to gestational hyperglycemia, and the application comprises the following steps: detecting the copy number of plasma mtDNA of offspring, and comparing the copy number with a preset threshold value to predict the risk that the offspring suffers from the congenital heart disease related to the gestational hyperglycemia. According to the invention, animal experiments find that plasma mtDNA level change has clear correlation with fetal congenital heart disease induced by hyperglycemia in gestation period for the first time, show good stability and consistency in the animal experiments, and can objectively reflect the heart state; therefore, the prediction method for reflecting the occurrence risk of the fetal congenital heart disease under the hyperglycemia state in the gestation period is established by taking the plasma mtDNA as the biomarker, can be applied to basic research, risk assessment and related technology development of heart dysplasia related to the hyperglycemia in the gestation period, and has relatively high practical value and popularization significance.

The invention belongs to the technical field of biology, and particularly relates to application of an SNP molecular marker to identification of dairy cows with high reproductive performance and assistant breeding. The nucleotide sequence of the SNP molecular marker is shown as SEQ ID NO.4. The single nucleotide site at the 481bp of the sequence is T or G, the single nucleotide site at the 485bp of the sequence is G or A, and the single nucleotide site at the 531bp of the sequence is C or T. The invention further discloses a preparation method of the SNP molecular marker. According to the invention, the application of the SIGLEC12 gene as the marker gene in the gestation period of the dairy cow is determined for the first time, and a new target is provided for research on the reproductive performance of the dairy cow. The provided SNP molecular marker and the identification method have the advantages of high accuracy, simplicity and convenience in operation, low detection cost and the like, high reproductive performance identification can be performed in the early stage of the dairy cow, and the breeding period is greatly shortened.

The invention discloses an application of an SNP genetic locus related to the number of days of a sow in a gestation period and a molecular marker in pig genetic breeding or identification, and relates to the technical field of pig genetic breeding and biologication.The SNP genetic locus is related to the number of days of the sow in the gestation period and located at the intron position in a LOXL2 gene, and the LOXL2 gene is located in a 7435867-7540704 base region of the 14th chromosome of a pig reference genome and located at the intron position of the LOXL2 gene. The genetic locus rs3471610676 provided by the invention is located at a No.7472833 basic group of a No.14 chromosome, namely the position of a No.36996 basic group in the LOXL2 gene, and the molecular marker is a basic group sequence containing the SNP genetic locus. The SNP genetic locus or the molecular marker can assist in shortening the number of days of the gestation period of the sow, provides a new marker resource for auxiliary selection of the breeding sow, and can be used for genetic breeding and identification of the breeding sow.

The invention provides an anticoagulant polypeptide HS9 and application thereof, and belongs to the technical field of functional polypeptides. The amino acid sequence of the anticoagulant polypeptide HS9 is as shown in SEQ ID NO: 1. The anticoagulant polypeptide HS9 has strong anticoagulant activity, has the efficacy of inhibiting thrombosis in gestation period and reducing bleeding risk, and also has the characteristics of simple structure and convenience in preparation. Therefore, the invention provides application of the anticoagulant polypeptide HS9 in preparation of antithrombotic drugs or drugs for preventing and / or treating blood coagulation dysfunction diseases.

The present application relates to the technical field of medical detection, in particular to a biomarker combination for diagnosing gestational diabetes mellitus and application thereof. The present application provides application of SHBG protein and Vaspin protein in preparation of a product for diagnosing gestational diabetes mellitus. When SHBG and Vaspin are used in combination for diagnosing gestational diabetes mellitus, the diagnostic efficiency is extremely high, the AUC value reaches 0.934, the accuracy is 0.91, the sensitivity is 0.872, and the specificity is 0.949. The biomarker combination of the present application can be used for early diagnosis of whether there is a risk of GDM, so as to provide a basis for timely intervention, reduce the complication risk of pregnant women and fetuses, and improve the health outcomes of mothers and infants.

The application belongs to the technical field of animal experiment models, and discloses a method for constructing a mouse model of autism spectrum disorder and application. Pregnant mice are orally exposed to different doses of 1-NP every day during the whole gestation period, the pregnant mice naturally give birth, and the offspring mice are evaluated for autism-like behaviors at PND28 after birth by using a three-chamber social experiment. The offspring mice are evaluated for autism-like behaviors at PND70 after birth by using a three-chamber social experiment. Maternal 1-NP exposure during pregnancy delays the migration of intermediate neurons in the forebrain of fetal mice, and the autism-like behaviors. 1-NP exposure during pregnancy down-regulates the expression of intermediate neuron migration-related genes by reducing DNA hydroxymethylation of the intermediate neuron migration-related genes. 1-NP exposure during pregnancy damages the synthesis of mitochondrial tricarboxylic acid cycle alpha-ketoglutarate (alpha-KG). Maternal alpha-KG supplementation during pregnancy improves the migration of intermediate neurons in 1-NP exposed fetal mice and the autism-like behaviors of offspring mice.

The use of an active folic acid substance in the preparation of a food product for helping regulate the intestinal flora of a mother and offspring thereof via ingestion by the mother, wherein the active folic acid substance is selected from 6S-5-methyltetrahydrofolic acid and a salt thereof, the mother is in the period of pregnancy and / or lactation, and the offspring is in the fetal period, infant period, and / or childhood.

The present application relates to the technical field of giant panda breeding, and discloses a method for judging the true or false pregnancy and the expected delivery date of a giant panda, which comprises the following steps: collecting and detecting the progesterone value of the urine sample of a pregnant giant panda every day after mating; counting the amount of excrement of the pregnant giant panda; taking the day when the progesterone value of the excrement of a giant panda reaches the peak as day 0, taking the number of days as the horizontal coordinate, and taking the progesterone value of the urine sample and the amount of excrement of the pregnant giant panda as the vertical coordinate, to construct a pattern diagram of the change of the progesterone value and the amount of excrement of the giant panda; and judging the true or false pregnancy and the expected delivery date of the giant panda by observing the pattern diagram. The true or false pregnancy of the giant panda can be judged by observing the decrease of food and the change of the amount of excrement of the giant panda after the C point, and the expected delivery date of the true pregnant giant panda can be predicted by the time after the progesterone value peak B point. Therefore, the management personnel can strengthen the management of the giant panda in the embryo implantation period, effectively prevent the early embryo loss of the giant panda, and improve the pregnancy rate of the giant panda and prevent the early abortion of the fetus.

The application belongs to the field of data processing, and particularly relates to a pregnancy-induced diabetes subtype analysis method based on causal enhancement, which comprises the following steps: S10: obtaining an original mixed data set of pregnancy-induced diabetes, pre-processing the original mixed data set to obtain a standardized numerical characteristic matrix, learning the potential structure between features on the numerical characteristic matrix by using a causal discovery algorithm, and generating a causal structure directed graph; S20: determining the causal weight of each feature by using a mean intervention mechanism and a cluster center distance difference measurement; S30: calculating the causal weighted numerical distance matrix of the numerical characteristics and the frequency-aware category distance of the category characteristics, and constructing a unified comprehensive distance space matrix; S40: based on the obtained comprehensive distance space matrix, a clustering algorithm is used for iterative updating, and a final subtype division result is output; by introducing a feature weighting mechanism guided by a causal structure, the application can effectively exclude the interference of redundant clinical indicators.

Systems, devices, and methods are provided for controlling, supporting, and / or elongating the cervix in order to treat pregnancy complications and / or delay the onset of labor, thereby prolonging the gestation period of the fetus and thereby preventing premature birth and delivery of the fetus. The cervical control system may include a main body configured for placement in the vagina. The main body has an internal passage configured for receiving the cervix therein, and an inflatable and / or expandable internal cuff extending from a portion of the internal passage. The internal cuff may be expandable inward toward the cervix, or inward toward the uterus, or both inward and inward, so that the internal cuff may engage with or grasp the cervix, thereby holding the cervical control system in place while compressing and closing the cervix, elongating the cervix, and / or adjusting the angle of orientation of the cervix.

The invention relates to a pregnancy staged movement interaction method and system based on multiple physiological parameters, and belongs to the technical field of medical health. Core interaction equipment is used for collecting movement scenes, storing standard movement data and processing and storing various monitoring data, movement recording is carried out, pregnancy stages are divided based on the various monitoring data, and the movement data are stored; constructing a database according to pregnancy stage data and standard motion data, configuring motion data in combination with the database and pregnancy characteristics, identifying core joint points by using an AI algorithm based on the standard motion data, tracking core joint point change data, and performing analysis according to the core joint point change data; the motion posture is prompted according to the analysis result, meanwhile, motion early warning and fetal state visual feedback are carried out based on various monitoring data, and display is carried out according to a preset mode, the blank of lack of special motion courses in different stages of pregnancy in the prior art is filled, and accurate motion guidance is provided according to physiological needs of all stages.