227 results about "Nanomedicine" patented technology

The invention relates to a reducible and degradable nano medicine-carrying micelle and a preparation method thereof, belonging to the fields of biomedical technology and nano medical technology. The reducible and degradable nano medicine-carrying micelle is characterized by having the following structural general formula: MPEG-CA-PCL, wherein the CA is poly-cystamine containing disulfide bonds, the MPEG is methoxy polyethylene glycol the molecular weight of which is 475-5000DA, and the PCL is polycaprolactone. The preparation method of the reducible and degradable nano medicine-carrying micelle comprises the following steps: (1) carrying out Michael addition to N,N'-bis (acrylic) cystamine and ethanolamine to obtain hydroxy poly-cystamine containing disulfide bonds, and then, carrying out ring opening reaction on caprolactone by utilizing a hydroxy group to obtain a CA-PCL polymer; and (2) mixing the CA-PCL polymer obtained in the step (1) and carboxyl-terminated MPEG, and adding paclitaxel (PTX) to prepare the nano medicine-carrying micelle through a dialysis method. The method is efficient and simple.

The invention relates to a preparation method and application of a reduction-response-type pegylation (PEG) nanomedicine composition. The nanomedicine composition is characterized by being prepared by coupling the PEG with a medicine via disulfide bonds which are sensitive in reduction. Thus, the water solubility of the medicine is improved; the in-vivo behavior of the medicine is also improved. Meanwhile, the full release and the activity of the medicine are ensured by utilizing the characteristic that the disulfide bonds in the PEG medicine, which are sensitive in reduction, can be specifically degraded in a tumor site. Thus, a good tumor treatment scheme is provided.

The invention provides synthesis for biodegradable amphiphilic block copolymerization antibacterial peptoids, a preparation method of antibacterial peptoid vesicles and applications of the antibacterial peptoid vesicles. The synthesis for the antibacterial peptoids comprises the following steps: a hydrophilic polypeptide chain segment containing an amino protective group, a hydrophobic polymer chain segment of which one end is amino-terminated and isocyanate radicals of a diisocyanate unit are connected through covalent bonds, and therefore the antibacterial peptoids are obtained; and the antibacterial peptoid vesicles are formed by self assembly of the antibacterial peptoids, and the antibacterial peptoid vesicles can be used as antibacterial agents or drug carriers to be applied to aspects such as clinical inflammation resistance, anti-cancer drug targeting release, or nanomedicine. The antibacterial peptoid vesicles select and use the biodegradable hydrophobic polymer chain segment to replace a hydrophobic amino acid chain segment in an antibacterial peptide, thus the antibacterial peptoid vesicles do not have cytotoxicity, and have excellent biocompatibility and biodegradability; and besides, the antibacterial peptoid vesicles have a broad-spectrum antibacterial property, and a membrane damage antibacterial mechanism which is similar to that of a natural antibacterial peptide, and therefore drug tolerance of germs is not easily induced.

The invention belongs to the field of nanometer medicine and biomedical engineering, and particularly discloses a nanometer vesicle capable of simultaneously achieving RNA interference and MR imaging and a preparation method and application thereof. The nanometer vesicle is based on amphipathy block polymer carrier linear polymine-polyactic acid. A hydrophobic cavity of the nanometer vesicle is used for performing loads on hydrophilic magnetic nanoparticles SPIO, siRNA is composited on the surface PEI, neural stem cells are effectively marked in vitro, nerve cell differentiation genes are restrained silently, stem cells are positioned in real time after in-vivo transplanting, the stem cells are promoted to be differentiated to nerve cells, the cerebral infarction treatment effect is improved, and the application prospect is wide.

The invention provides anamphiphilic multi-block antibacterial peptide copolymer, and a preparation method and application thereof. According to the amphiphilic multi-block antibacterial peptide copolymer,a hydrophobic amino acid block and a hydrophilic amino acid block are selected and usedfor simulating and replacingan amino acid composition in natural antibacterial peptide, therefore, excellentbroad-spectrum antibacterial performance, low cytotoxicity, excellent biocompatibility and biodegradability are achieved, an antibacterial mechanism of the amphiphilic multi-block antibacterial peptide copolymer is the same as that of the natural antimicrobial peptide, bacteria die by destroying bacterial cell membranes, andthe bacteria is not prone to being inducedto produce drug resistance.According to synthesis steps of the preparedamphiphilic multi-block antimicrobial peptide copolymer is simpler, efficient and lower in production cost compared with step-by-step synthesis reaction, and the amphiphilic multi-block antibacterial peptide copolymer can be used as an antimicrobial agent or drug carriers to be used in aspects of clinical anti-infection, targeted release of anticancer drugsor nanomedicine.

Provided herein is a method for manipulating the surface density of functional molecules conjugated to nanoparticles, which method including incubating nanoparticles with nucleotides to form nucleotide-coated nanoparticles, adjusting buffer and salt concentration of the conjugation media, adding thiolated molecules in the conjugation media to incubate with the nucleotie-coated nanoparticles, and adding thiolated oligo(ethylene glycol) in the conjugation media to cease the conjugation process of thiolated molecules to nanoparticles. The method is simple, efficient and cost effective, and the surface density of functional molecules can be quickly manipulated in a wide range for various applications, such as biosensing, molecular diagnostics, nanomedicine, and nano-assembly.

The present invention relates to a nano co-delivery system for co-delivering a NO donor and nanomedicine. The system comprises a nanocarrier, the NO donor and the nanomedicine, wherein the nanocarrieris a mesoporous silica nanoparticle, the NO donor is nitrosothiol bonded to the surface of the nanocarrier, and the nanomedicine is loaded in a mesoporous channel of the nanocarrier. The invention also relates to a preparation method of the co-delivery system and application of the co-delivery system in promoting deep permeation of the nanomedicine in tumor tissue. After intravenous administration, the nano co-delivery system is gathered in the tumor tissue through an EPR effect, nitrosothiol is decomposed to release NO, the expression and activity of MMP are improved through a process of NO,ONOO and MMP, tumor matrix collagen is degraded, the deep permeation of the nanomedicine in the tumor tissue is promoted, and an anti-tumor curative effect is improved.

The invention relates to a macromolecule based preparation method of pH-responsive metal-organic coordination polymer in the technical field of nanomedicine, comprising the steps of: dissolving the biocompatible macromolecule in the deionized water, orderly adding the medicinal solution and the metal nitrate solution while stirring and adjusting the pH to be neutral with the alkali, then orderly adding the poor solvent and the metal sulfate solution, and centrifugally washing and drying to obtain the pH-responsive metal-organic coordination polymer. In the invention, the medicine is prepared into the nanoscale metal-organic coordination polymer by means of the biocompatible macromolecule and the metal ion, and the invention can perform controllable release to the medicine according to the small change of the pH.

The invention relates to the cross field of chemical engineering, materials and nanomedicines, and specifically discloses a multi-modality imaging probe structure and a preparation method thereof. A multi-modality imaging probe is a microbubble with a putamen structure, a shell membrane material is composed of a high polymer material and a phospholipid material which are biodegradable and good in biocompatibility, water-solubility quantum dot solutions with different fluorescent characteristics are embedded at the center, a perfluor carbon alkyl gas is fed, and the multi-modality probe which is controllable in grain diameter, good and stable in dispersion and easy to store are prepared by a double-emulsion-freeze-drying gas feeding method. According to the multi-modality imaging probe structure the preparation method thereof, the microbubble is a reticuloendothelial system specificity contrast agent, the fluorescent imaging is achieved, the enhancement for ultrasonoscopy and magnatic resonance imaging (MRI) display and the microbubble multi-mode imaging can be achieved, and the multi-modality imaging probe structure the preparation method thereof have a wide application prospect.

The present invention relates to targeted nanocarriers - also termed nanomedicines - and methods of preferentially, or actively, targeting and delivering a tool for gene transfer or genome editing (i.e., a plasmid or a restriction enzyme - such as a zinc finger nuclease, a CRISPR / Cas system, or a TALEN) or a tool for gene silencing or post-transcriptional regulation of gene expression (i.e., a microRNA, a siRNA, a mRNA, an antisense oligonucleotide, or a sense oligonucleotide) to a range of mammalian cell species. Cell- specific targeting is achieved by using nanocarriers featuring suitable targeting anchors having a targeting moiety that can be a carbohydrate, an antibody or an antibody fragment, a non-antibody protein derivative, an aptamer, a lipoprotein or a fragment thereof, a peptidoglycan, a lipopolysaccharide or a fragment thereof, or a CpG DNA. Such targeting anchors may or may not include a polymeric spacer like polyethylene glycol. The nanomedicines shall allow to therapeutically address a range of mammalian disease entities via various application routes. These indications include malignant diseases, autoimmune diseases, inherited disorders, metabolic disorders, or infectious diseases.