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Nanoparticle drug loading system and preparation method and application thereof

A nano-particle and nano-drug loading technology, which is applied in the direction of pharmaceutical formulations, antineoplastic drugs, and drug combinations, can solve the problems of wasting macrophages, not utilizing the natural immune cell functions of macrophages, increasing process and cost, etc.

Inactive Publication Date: 2020-03-06
SHENZHEN INST OF ADVANCED TECH
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0005] Although the above-mentioned existing technologies have a certain effect on the treatment of tumors through macrophage drug loading and macrophage targeting systems, these methods only use macrophages as carriers to deliver drugs to tumor sites to play a role. Does not take advantage of the natural immune cell function of macrophages
This not only increases the process and cost of preparation preparation, but also wastes the role of macrophages

Method used

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  • Nanoparticle drug loading system and preparation method and application thereof
  • Nanoparticle drug loading system and preparation method and application thereof
  • Nanoparticle drug loading system and preparation method and application thereof

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0042] Example 1 Preparation of macrophages loaded with nanoparticles on the surface

[0043] ① Synthesis of nanoparticles wrapped with maleamide bonds by ultrasonic method;

[0044] ② Take out and induce primary macrophages from the mouse spinal cord;

[0045] ③ When the growth state of macrophages is good, count 2×10 6 The left and right macrophages were placed in a 1.5mL centrifuge tube, and 1mM TCEP solution (diluted with phosphate buffer) was added to it, at 37°C, 5% CO 2 Incubate in the incubator for 20 minutes, during which the centrifuge tube is inverted every 10 minutes, and then washed 3-4 times with phosphate buffer;

[0046] ④ Transfer the nanoparticles and the treated macrophages into a 15mL centrifuge tube, continue to drop the phosphate buffer to a total volume of 4mL, then put it in a shaker at 37°C, rotate at 120 for 20 minutes, and finally put the mixed liquid into the tube. Centrifuge at 1000 rpm for 4 minutes to successfully load the particles on the sur...

Embodiment 2

[0047] Performance and characterization of macrophages loaded with nanoparticles on the surface of Example 2

[0048] 1. SEM images of macrophages loaded with nanoparticles

[0049] First, the solution of macrophages loaded with nanoparticles on the surface was diluted 10 times, and then the sample was prepared according to the scanning electron microscope sample preparation method, and then observed under the scanning electron microscope. It was found that nanoparticles loaded on the surface of macrophages, such as figure 1 shown.

[0050] 2. Laser confocal images of macrophages loaded with nanoparticles

[0051] Firstly, the solution of macrophages loaded with nanoparticles on the surface was diluted 100 times and added dropwise into a confocal small dish, and then the confocal small dish was placed under a laser confocal microscope for observation. The results showed that nanoparticles were loaded on the surface of macrophages, as figure 2 shown.

[0052] 3. Detection...

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Abstract

The preparation provides a nano drug loading system, which is simple is a preparation method, mature in process, stable in property and good in biocompatibility. According to the nano drug loading system, nano particles are coupled to the surfaces of macrophages through maleic amide bonds; the tumor is jointly treated through the immune regulation function of macrophages and the functions of the nanoparticles, so that the anti-tumor curative effect is improved; the problem that in the prior art, drug micromolecules cannot be effectively enriched and targeted at tumor sites is solved, and the nano drug loading system has important application prospects in the nano medical field and the tumor treatment field.

Description

technical field [0001] The invention belongs to the field of nanomedicine, and in particular relates to a drug-carrying system loaded with nanoparticles on the surface of macrophage cells, a preparation method thereof and an application in tumor immunotherapy. Background technique [0002] Tumor immunotherapy is called the fourth tumor treatment method after surgery, chemotherapy and radiotherapy. Different from traditional treatment methods, tumor immunotherapy is a treatment method that targets the human immune system rather than directly targeting tumors. It stimulates or mobilizes the body's immune system and enhances the anti-tumor immunity of the tumor microenvironment, thereby controlling and killing tumor cells. . In recent years, cellular immunotherapy has attracted extensive attention as the main tumor immunotherapy. Cellular immunotherapy, which is called adoptive immune cell therapy, refers to the delivery of immune cells (specific and non-specific) with anti-t...

Claims

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Application Information

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IPC IPC(8): A61K9/50A61K47/46A61K47/34A61K45/00A61P35/00
CPCA61K9/5031A61K9/5063A61K45/00A61P35/00
Inventor 蔡林涛周海梅刘兰兰何华美梁锐晶
Owner SHENZHEN INST OF ADVANCED TECH
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