153 results about "Tumor microenvironment" patented technology

A brain glioma full-slice pathological image intelligent analysis method based on a double-path analysis framework specifically comprises the following steps: S1, preprocessing full-slice images, dividing each full-slice image into different foreground tissue areas and background areas by using a channel threshold segmentation algorithm, and performing image segmentation on the foreground tissue areas and the background areas; cutting the foreground tissue region into a plurality of image blocks with fixed sizes, and extracting a feature vector of each image block to form a feature cluster; s2, establishing a dynamic sampling mechanism, performing dynamic multi-instance learning on the feature cluster of each full-slice image by using a two-stage attention network, and finally obtaining slice-level feature representation of the full-slice image; and S3, quantifying tumor microenvironment spatial relationship information to obtain a feature representation of a spatial relationship quantization graph, and splicing the feature representation with the slice-level feature representation in the S2 to obtain a final prediction result. According to the method, the prediction efficiency and robustness are improved, and the medical decision speed and quality are improved.

The invention relates to a method for detecting Breg cells and Tfh cells in a chest adenoma tissue microenvironment, which comprises the following steps of: coupling quantum dots with a specific antibody, dyeing a chest adenoma tissue slice sample, and specifically connecting the specific antibody with antigens on the Breg cells and / or Tfh cells in the slice sample cells, so as to quantify or position the Breg cells and / or Tfh cells by the quantum dots. According to the method disclosed by the invention, the existing multicolor immunofluorescence technology is improved, and a secondary antibody dyeing link is omitted by using a method of carrying the antibody by the quantum dots, so that the efficiency is improved; the quantum dots are used for replacing fluorescent dye, direct marking of tissue slices can be achieved, local positioning of Breg cells and Tfh cells in a thymoma tumor microenvironment is achieved, and biological behaviors of the Breg cells and the Tfh cells can be determined conveniently; the immune condition can be evaluated by detecting the ratio condition of Breg / Tfh cells in the breast adenoma tumor microenvironment of a patient in vitro.

The invention relates to the technical field of biological medicine, in particular to immunogen cell death enhanced nano-vesicle hydrogel and application thereof. The invention provides a 3D-printable multifunctional synergistic immunogen cell death enhanced nano-vesicle hydrogel, which is characterized in that SerMA hydrogel is used as a matrix, cell membrane nano-vesicles (N (at) ILO) loaded with a photothermal agent IR1061, a chemotherapeutic drug OXA and a tumor acidic microenvironment regulator Lon are encapsulated, initial tumor immunogen cell death is triggered through a photothermal effect, and the tumor immunogen cell death enhanced nano-vesicle hydrogel can be used for 3D printing. OXA further enhances the ICD effect, Lon regulates the acidic tumor microenvironment to improve the T cell activity, and postoperative multi-mode synergistic anti-tumor treatment is achieved; meanwhile, the hydrogel also has excellent photocuring 3D printing capability, can be used for individually constructing a filling structure matched with a postoperative defect area, has a wide application prospect, and provides a new strategy and theoretical basis for postoperative comprehensive treatment of choroidal melanoma.

The invention provides preparation of ROS responsive lipidosome and application of the ROS responsive lipidosome in anti-pancreatic cancer drugs, and belongs to the technical field of ROS responsive lipidosome preparation. The liposome can synergistically deliver formononetin and salvianolic acid B, overcomes the defects of formononetin and salvianolic acid B in the aspects of solubility, stability and bioavailability, and improves the curative effect of formononetin and salvianolic acid B in pancreatic cancer treatment. The ROS-responsive formononetin liposome and the salvianolic acid B liposome are wrapped by the cell-derived nano-vesicles through co-extrusion with the nano-vesicles on the outer layer, so that the ROS-responsive liposome is prepared. The liposome can be used for preparing anti-pancreatic cancer drugs, realizes targeted treatment of pancreatic cancer, inhibition of pancreatic cancer cell proliferation, promotion of pancreatic cancer cell apoptosis and improvement of pancreatic cancer tumor microenvironment energy metabolism disorder, and has the advantages of high biological safety, controllable drug release and the like.

The invention discloses a construction method of a model for predicting an esophageal squamous cell carcinoma intervention effect. The construction method comprises the following steps: acquiring detection data of a predictive factor in a sample; the predictive factors at least comprise the content or the expression level of a depletion marker of a functional CD8 T cell in a sample and the content or the expression level of a C1QC + macrophage immune checkpoint molecule; and predicting the intervention effect of the esophageal squamous cell carcinoma according to the detection data of the predictive factor. Establishing a space proteomics map of a complex space tissue of a tumor microenvironment (TME) during treatment; key spatial features which are related to specific cell behaviors and influence treatment results are determined and layered; a powerful prediction model of drug response is developed by utilizing a machine learning technology, and the area under curve (AUC) can reach 97%.

The invention discloses a polyethylene glycol modified gold and manganese dioxide nano particle and a preparation method and application thereof, and belongs to the technical field of biological medicine, the polyethylene glycol modified gold and manganese dioxide nano particle (GMCN and PEG) has good biocompatibility under neutral physiological conditions, can relieve tumor hypoxia and increase generation of active oxygen in acidic TME, and has a good anti-tumor effect. The radiotherapy sensitivity is improved; and immune cell death is induced. Meanwhile, a cGAS-STING signal channel can be activated, dendritic cell maturation, macrophage M1 polarization and T cell infiltration are promoted, and the immunosuppression state in TME is counteracted. In addition, the GMCN-coated PEG also has an MR-CT bimodal imaging enhancement function, and integration of diagnosis and treatment is realized. The nano sensitizer disclosed by the invention has huge potential in the aspects of improving the radiotherapy curative effect, remodeling the tumor microenvironment, promoting the anti-tumor immunity, enhancing the biomedical imaging and the like.

The invention provides an organoid chip for screening micro-ecological drugs as well as application and a preparation method of the organoid chip, and relates to the technical field of biological medicines. The invention provides a chip integrating intestinal epithelium, a prostate cancer organ and a bladder cancer organ, which is used for researching how microecological preparations, such as probiotics, metabolites and the like, affect tumor microenvironment and immunotherapy effects. The chip can simulate interaction of intestinal tract-tumor axis, and provides an in-vitro evaluation system for precise tumor immunotherapy.

The invention relates to a double-response EGFR (epidermal growth factor receptor) targeted paclitaxel liposome based on heat shock protein, which adopts a pH sensitive design and is doped with heat shock protein 90 (HSP90) responsive molecule gambogic acid (GA), the surface of the liposome is modified with an EGFR antibody, and the core of the liposome is packaged with paclitaxel (PTX). According to the double-response EGFR targeted paclitaxel liposome of the heat shock protein, triple negative breast cancer cells are accurately locked through a double targeting strategy, and cell surface specific recognition is achieved through an EGFR antibody; gambogic acid GA responds to HSP90 overexpression in cells to promote drug release; the pH sensitive characteristic ensures that the medicine is quickly released in an acidic tumor microenvironment, the treatment effect is enhanced, and normal cell damage is reduced. According to the invention, the specific quick response release of the medicine is realized, the treatment accuracy and safety are improved, and the preparation method has a remarkable application prospect in the aspect of preparing the medicine for treating the triple-negative breast cancer.

An antibody gold nanocage drug delivery system, a preparation method, and a use. LA-DOX-mPEG and a gold nanocage are first prepared independently; reaction between LA-DOX-mPEG and the gold nanocage is enabled to obtain DOX@GNC; then HS-PEG-COOH and an antibody are added for reaction to obtain the antibody gold nanocage drug delivery system. In addition to the advantages of nano drug delivery systems in the prior art, the gold nanocage drug delivery system further has the active targeting capability for tumor cells and can reduce the toxic and side effects of chemotherapeutic drugs on normal cells; pH responsive DOX drug release in an acidic tumor microenvironment can improve drug accumulation in tumor tissue. In addition, under the irradiation of near-infrared light, the gold nanocage converts light energy into heat energy, and the pH responsive drug release combined with high temperature enables rapid pulsed release of DOX carried by the gold nanocage, thereby achieving synergistic treatment of photothermal therapy and chemotherapy.

The invention discloses a functionalized culture medium, a culture medium composition and a culture method for NK cell amplification, and belongs to the field of biological medicine. Aiming at the problem that the activity and the purity of the NK cells cannot be considered at the same time in the existing NK cell in-vitro amplification, the invention provides a functionalized culture medium for NK cell amplification, and the functionalized culture medium comprises a proliferation culture medium and an activation adaptation culture medium; the proliferation culture medium and the activated adaptive culture medium both comprise IL-10, and the concentration of the IL-10 in the proliferation culture medium is lower than that of the IL-10 in the activated adaptive culture medium. IL-10 is added into a proliferation culture medium and an activation adaptation culture medium, the concentration of IL-10 in the two culture mediums is different, and the synergistic effect of high purity (larger than 95%) and high activity (the killing rate is larger than 75%) is achieved by inhibiting T cell proliferation and enhancing the anti-tumor microenvironment capacity of NK cells.

The invention discloses a preparation method of oxygen deficit and H2O2 dual-response COF nano-enzyme and application of the oxygen deficit and H2O2 dual-response COF nano-enzyme in treatment of pancreatic cancer, and belongs to the technical field of application materials. The COF is prepared from 1, 3, 6, 8-tetra (4-formylphenyl) pyrene (Py-CHO), p-diaminoazobenzene (Azo-NH2), ferroferric oxide (Fe3O4), dopamine hydrochloride (DA) and hyaluronic acid (HA), and the COF is prepared from the following raw materials in parts by weight: 1, 3, 6, 8-tetra (4-formylphenyl) pyrene (Py-CHO), p-diaminoazobenzene (Azo-NH2), ferroferric oxide ( Under a tumor microenvironment (TME), the CFPH can be split due to reductase existing in an anoxic environment, released Fe3O4 can react with H2O2 in TME to generate ROS, and pancreatic cancer cells are effectively killed. The COF prepared by the invention is low in cost, simple and convenient in preparation method and good in biocompatibility, and has a wide application prospect in the aspect of pancreatic cancer treatment.

Activation of STimulator of INterferon Genes (STING) triggers cytokine production and facilitates tumor antigen cross-presentation. In an embodiment of the present invention, STING-dependent innate immune signaling pathway activators (STAVs) together with Replicants including mRNA adapted to express an antigen can be delivered to antigen presenting cells (APC's) using lipid nanoparticle formulations. In various embodiments of the present invention, the range of cancers amenable to STAV / Replicant therapy can be extended using a non-cell-based nanoparticle strategy that effectively delivers the STAV / Replicant into the Tumor Micro Environment (TME) to potently generate anti-tumor cytotoxic T cell activity together with humoral immune responses. The STAV / Replicant formulations can be introduced into solid tumors present in the subject. Alternatively, the STAV / Replicant can be introduced through direct inoculation, intramuscularly, or intravenously. The lipid nanoparticles stick to the tumor cells and are co-phagocytosed to activate STING in APC's.

This invention discloses a composite nanodelivery system encapsulated in a fusion membrane, its preparation method, and its applications. The nanodelivery system comprises a Ti3C2 / TiO2 / CuInS2 composite material, oxaliplatin loaded thereon, and a fusion membrane encapsulating the outer layer; the fusion membrane is formed by the fusion of Fusobacterium nucleatum extravesicles and M1 macrophage membranes. This invention also discloses a method for preparing the nanodelivery system, including the steps of preparing the Ti3C2 / TiO2 / CuInS2 composite material, preparing the fusion membrane, loading oxaliplatin, and encapsulating it in the fusion membrane. The nanodelivery system prepared by this invention exhibits pH-responsive drug release characteristics, generating H2S in the tumor microenvironment and H2 under light irradiation, while also possessing photocatalytic activity. This invention combines chemotherapy, photoelectrocatalytic therapy, and the regulation of multiple active substances, and can be used to prepare drugs for treating malignant tumors of the digestive system.

The invention relates to the technical field of physiological monitoring, in particular to a hepatocellular carcinoma treatment effect analysis system and method based on metabolism comprehensive analysis, and the method comprises the steps: obtaining the evolution condition of hepatocellular carcinoma, the composition condition of drugs and the composition condition of metabolites; drug absorption and transformation analysis is carried out based on the composition condition of the drug and the marking condition of the composition condition of the metabolite, future drug transformation component prediction is carried out based on the drug absorption and transformation analysis result and the evolution condition of the corresponding hepatocellular carcinoma, and drug treatment effect early warning is carried out based on the future drug transformation component prediction. The first-pass effect is corrected through mass spectrum imaging and enzyme activity, the inhibition effect of the tumor microenvironment on drug transformation is quantified, prediction is dynamically adjusted through the future tumor anomaly score ratio, and the accuracy of the time of drug replacement is improved.

The invention discloses a multifunctional double-drug delivery system based on metal collaborative treatment and application of the multifunctional double-drug delivery system, and belongs to the technical field of biological medicine. The double-drug delivery system is double-drug delivery lipidosome and comprises metal ions, a lipidosome membrane and active ingredients, the metal ions are adsorbed on the surface of the lipidosome membrane, and the active ingredients are wrapped in the lipidosome membrane; the metal ions are divalent metal ions; the active component consists of an STING agonist and a traditional Chinese medicine active monomer component. According to the double-drug delivery system, the STING agonist and the traditional Chinese medicine anti-inflammatory components are co-loaded through lipidosome, and multi-dimensional remodeling of a tumor microenvironment is achieved through the double effects of exciting tumor immunity and inhibiting inflammatory reaction; and meanwhile, by introducing metal ions, the lysosome escape efficiency of a liposome drug delivery system is effectively enhanced, so that the intracellular delivery efficiency of the drug is improved.

The invention relates to the technical field of biomedical detection, and discloses a system for detecting extracellular matrix free metalloproteinase and a soluble receptor. Comprising a polypeptide substrate nanopore sensing module used for detecting the enzyme activity of free metalloproteinase in an extracellular matrix; the electrophoretic analysis module is used for detecting a soluble receptor in an extracellular matrix, applying an electric field to a tumor microenvironment sample, determining the inter-terminal resistance of the sample by using an inter-terminal resistance detection device, and detecting the soluble receptor by comparing the resistance change according to the characteristic that the existence of the soluble receptor causes the additional resistance increase of the sample; and the data processing and judging module is electrically connected with the polypeptide substrate nanopore sensing module and the electrophoretic analysis module respectively, and is used for receiving the free metalloproteinase activity detection result and the soluble receptor detection result, and judging the time interval of cellular immune escape according to a preset rule in combination with cellular morphology observation information.

Activation of STimulator of INterferon Genes (STING) triggers cytokine production and facilitates tumor antigen cross-presentation. In an embodiment of the present invention, STING-dependent innate immune signaling pathway activators (STAVs) together with Replicants including mRNA adapted to express an antigen can be delivered to antigen presenting cells (APC's) using lipid nanoparticle formulations. In various embodiments of the present invention, the range of cancers amenable to STAV / Replicant therapy can be extended using a non-cell-based nanoparticle strategy that effectively delivers the STAV / Replicant into the Tumor Micro Environment (TME) to potently generate anti-tumor cytotoxic T cell activity together with humoral immune responses. The STAV / Replicant formulations can be introduced into solid tumors present in the subject. Alternatively, the STAV / Replicant can be introduced through direct inoculation, intramuscularly, or intravenously. The lipid nanoparticles stick to the tumor cells and are co-phagocytosed to activate STING in APCs.

The present invention relates to methods and conjugates to improve the penetrability of biological substances into the tumour microenvironment (TME) for therapeutic purposes, suitably the agent comprises at least: i a therapeutic domain; ii. a cleavable domain; and iii. a stabilisation domain.

The invention provides a lung cancer microenvironment ecological characteristic detection and curative effect evaluation system, and relates to the technical field of lung cancer prognosis evaluation, and the system comprises a reading module which is used for reading slice information of a subject; the boundary construction module is used for constructing a region boundary according to the tumor region in the slice information; identifying tumor infiltrating lymphocytes (TILs for short) from the slice information, and determining the distribution type of the tumor infiltrating lymphocytes in combination with the distribution characteristics and the region boundary of the tumor infiltrating lymphocytes; the method comprises the following steps of: obtaining slice information, identifying a three-level lymphatic structure (TLS for short) from the slice information, and determining a distribution type of the three-level lymphatic structure in combination with distribution characteristics and a region boundary of the three-level lymphatic structure; and the evaluation module is used for performing prognosis effect prediction on the subject based on the distribution type of tumor infiltration lymphocytes and the distribution type of the three-level lymphatic structure, deeply analyzing the immune condition of a tumor micro-environment (TME for short), and improving the accuracy of prognosis evaluation.

The present invention relates to the field of biomedicine, and in particular to immunogenic cell death-enhancing nanovesicle hydrogels and their applications. The present invention provides a 3D-printable, multifunctional, synergistic immunogenic cell death-enhancing nanovesicle hydrogel. The hydrogel uses SerMA hydrogel as a matrix and encapsulates cell membrane nanovesicles (N@ILO) loaded with the photothermal agent IR1061, the chemotherapy drug OXA, and the tumor acidic microenvironment regulator Lon. The hydrogel triggers initial tumor immunogenic cell death through the photothermal effect, further enhances the ICD effect through OXA, and modulates the acidic tumor microenvironment through Lon to increase T cell activity, achieving multimodal synergistic anti-tumor therapy after surgery. The hydrogel also possesses excellent photocurable 3D printing capabilities and can be used to construct personalized filling structures that match postoperative defect areas. The hydrogel has broad application prospects and provides a new strategy and theoretical basis for the comprehensive postoperative treatment of choroidal melanoma.

The application provides a tumor immune microenvironment multi-cell recognition and spatial analysis system, comprising: an intrinsic signal generation module generating an initial intrinsic signal vector; a preliminary functional marker module generating a preliminary phenotype classification label map based on spatial centroid coordinates of cell objects; a functional neighborhood construction module calculating and generating a functional neighborhood feature vector; a composite feature splicing module constructing a composite state feature vector; an effective function determination module decoding to obtain a continuous value feature vector output by a function determination model representing the final function strength under the regulation of the microenvironment; and a functional map rendering module rendering to generate a cell effective function map under the regulation of the environment. The application solves the problem that, in the prior art, when interpreting cell functions, the intrinsic state information of the cells and the local functional microenvironment context information cannot be effectively fused, resulting in deviation in the description of the spatial distribution map of tumor microenvironment function heterogeneity.

The embodiment of the invention provides a TPD52 immunomodulatory effect research method and system, and the method comprises the steps: obtaining single cell transcriptome data of a breast cancer tumor microenvironment; on the basis of the data, identifying the TPD52 as the immune regulation key dangerous gene by utilizing a machine learning algorithm; verifying the association of TPD52 expression and patient prognosis in a multi-center queue; and based on the function annotation and the body appearance type experiment of the TPD52, confirming the immunomodulatory effect of the TPD52, and outputting a comprehensive evaluation report of the immunomodulatory effect of the TPD52. According to the invention, a machine learning method and scRNA-seq are utilized to explore the effect of TPD52 as a key immunomodulatory factor in BRCA, and the application has important significance on tumor behavior and patient prognosis.

The invention discloses preparation and application of a ternary carrier-free injectable hydrogel formed by co-assembling glycyrrhizic acid, norcantharidin and metal copper ions. The hydrogel can form gel under the condition of not adding auxiliary materials, has excellent gel material properties such as injectability, heat sensitivity and stability, and can be used as an ideal anti-tumor drug to be clinically applied. The hydrogel has the effects of inducing tumor cell apoptosis and copper death and resisting inflammation, and cooperatively regulates and controls a tumor microenvironment; and laser irradiation is combined to assist in enhancing the Fenton-like effect, so that chemical kinetic treatment of tumors is realized. In a Hepa1-6 tumor-bearing liver cancer mouse model, the hydrogel has an excellent anti-tumor effect, and the drug effect is obviously superior to that of each monomer drug group. In addition, the hydrogel has good biocompatibility, and is a novel anti-tumor hydrogel which is simple to prepare, green and has clinical transformation potential.

Disclosed is an application of a mechanical force sensitive macrophage subset in pancreatic cancer diagnosis or prognosis evaluation, in which it is found that a number of mechanical force sensitive macrophages of CD68+p-PYK2+YAP1+ in pancreatic cancer is obviously higher than that of an adjacent normal tissue, and the number of the macrophages is in positive correlation with a pancreatic cancer process, so that the mechanical force sensitive macrophages can be used as a pancreatic cancer diagnosis and prognosis index. An elasticity modulus of the cells is reduced by inhibiting a mechanical force check point PYK2 of monocytes / macrophages, and differentiation of the monocytes into the macrophages is inhibited, so that a tumor micro-environment of the pancreatic cancer is improved. Growth of the pancreatic cancer is remarkably inhibited through combined treatment, and the lifetime of mice with the pancreatic cancer is remarkably prolonged.

The present invention describes novel drug-carrying nanoparticles called bionanocarriers capable of modulating the cellular redox system and targeting the cancerous tumor.Specifically, the invention relates to a nanomedicine strategy for combating cancerous tumor cells, a strategy that aims to target the tumor, and consists of providing quercetin-linked magnetite nanoparticles (Q): (MNPs Q), and 3,5-dimaleylbenzoic acid-linked magnetite nanoparticles (A3'5DMB): (MNPs A3'5DMB)M; both types of nanoparticles were subsequently encapsulated with chitosan (Qs) and O-carboxymethyl chitosan (O-CMQs), functionalized with 11-mercaptoundecanoic acid (MUDA), and finally the anti-ABCC3 antibody was attached to the polymer phase, which was used as a selective driver of the delivery process of the encapsulated nanoparticles, thus carrying out the construction of the bionanocarriers: BNC-1: MNPs-Q-Ab; BNC-2: MNPs-a3'5DMB-Ab.Its manufacturing method and the tests of its biological functionality, its stability, the degradation of the encapsulation for the tumor microenvironment, the controlled release profile of the drugs Q and A3'5DMB, selective cell uptake, intracellular localization, cytotoxic effect on steroids of hepatocellular carcinoma cells with altered redox balance and induction of apoptosis, as well as its safety on normal cells, are described.

The present invention discloses the application of calcium ion-dependent secretory activation protein 1 in NK cell therapy. Through the study of tumor cells, the present invention found that the killing ability of NK cells overexpressing CAPS1 on tumor cells was significantly enhanced, indicating that CAPS1 has the effect of inhibiting tumor progression; multicolor immunohistochemistry found that CAPS1 molecules were lowly expressed in NK cells in the tumor microenvironment. In animal experiments of tumor modeling, the results showed that the tumor size of tumor cells co-cultured with NK cells of CAPS1 gene knockout mice was significantly larger than that of wild-type mice, and it was also concluded that CAPS1 has the effect of inhibiting tumor progression. The present invention proposes a new use of CAPS1 in tumor treatment, which provides good candidate drug targets and prognostic indicators for the clinical treatment of solid tumors such as liver cancer, lung cancer, colon cancer, melanoma, and blood system tumors such as B cell lymphoma and T cell lymphoma, and has good application prospects.

There is provided a gastric tissue tumour model that is three-dimensional comprising from 0.8 to 1.2 w / v % alginate, from 1.7 to 2.3 w / v % gelatin and gastric decellularized extracellular matrix (dECM). The gastric tissue model is for example an esophageal cancer tumour model which advantageously replicates the tumour microenvironment for esophageal cancer and can be used to perform a three-dimensional culture of esophageal tumour cells or metastatic cells that often metastasize into the esophagus.

The invention provides application of a GPX1 inhibitor combined with a copper death inducer in preparation of a cold tumor treatment medicine. The key node effect of GPX1 in cold tumor metabolism reprogramming is found and utilized. In a cold tumor microenvironment deficient in glutamine, tumor cells often resist a copper death inducer. Through targeted inhibition of GPX1, the sensitivity of cold tumor cells to copper ion toxicity is significantly enhanced, and the drug resistance problem caused by metabolic pressure is effectively overcome.

The invention provides a marker for detecting gastric cancer immunotherapy drug resistance, application of the marker, a model and a use method of the model, provides remarkable influence of a low-oxygen tumor microenvironment on gastric cancer immunotherapy drug resistance, and highlights key effects of FOXO3a and PD-L1 expression in a mediated immune escape mechanism. An innovative microfluidic tumor chip model is adopted, the in-vivo tumor hypoxia environment is successfully simulated, and how FOXO3a up-regulation induced by the hypoxia condition causes PD-L1 expression enhancement and further causes immunotherapy drug resistance is disclosed.

The invention relates to the technical field of biology, in particular to an in-vivo editing and metabolism reprogramming nano drug-loaded material for targeting macrophages as well as a preparation method and application of the in-vivo editing and metabolism reprogramming nano drug-loaded material. On the basis of a metal organic framework (MOF) material, CAR mRNA molecules targeting DLL3 are loaded in the MOF material, a macrophage-derived cell membrane is coated outside the MOF material, and the nano drug-loading material with the macrophage targeting capability is constructed. The material has the effects of specifically swallowing and killing tumor cells, improves the immunosuppression effect of a tumor microenvironment by utilizing the immune activation effects of succinic acid and manganese, and synergistically promotes the killing effect on small cell lung cancer. The macrophage in-vivo editing technology designed by the invention is a novel safe, efficient, simple and convenient tumor immunotherapy technology, overcomes the defect that CAR-T cannot enter the interior of a solid tumor, and solves the problem of tedious cell in-vitro amplification and gene editing procedures in the conventional CAR immunotherapy process; and a new strategy is provided for immunotherapy of small cell lung cancer with limited treatment means.