Carrier-free co-assembled tumor targeting anti-cancer nano medicine as well as preparation method and application thereof

A nano-drug and tumor-targeting technology, applied in the field of biomedicine, can solve the problems of complex carrier nano-system, unclear mechanism of action, and unclear metabolism, so as to solve the problems of unclear metabolism in vivo, complex solution system, and good tumor treatment effect Effect

Inactive Publication Date: 2017-09-15
FUZHOU UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

The present invention forms a co-assembled nanomedicine with targeted anticancer activity through π-π stacking, hydrophobicity and electrostatic force to solve the complex, difficult quality control, unclear mechanism of action and metabolic problems of artificially synthesized carrier nanosystems in the prior art. In order to achieve the goal of synergistic treatment of tumors

Method used

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  • Carrier-free co-assembled tumor targeting anti-cancer nano medicine as well as preparation method and application thereof
  • Carrier-free co-assembled tumor targeting anti-cancer nano medicine as well as preparation method and application thereof
  • Carrier-free co-assembled tumor targeting anti-cancer nano medicine as well as preparation method and application thereof

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0060] The preparation method of ursolic acid nano micelles

[0061] Accurately weigh 0.00456g of UA powder, dissolve it in 1ml of methanol, and ultrasonically dissolve it to form a 10mM ursolic acid methanol solution; take 100μL of the above solution, and add it dropwise to 2ml of secondary water during stirring (note: drop Stir at a high speed during the addition process, and the dropwise addition time is 30s), at this time, the concentration of UA in the solution is 500 μM, and then stir for 8 minutes to obtain UA nanomicelles.

[0062] The average particle diameter of the UA nanomicelle prepared in this embodiment is about 98.32 nanometers, and the particle diameter diagram is as follows figure 1 shown.

[0063] The contrast imaging picture of the UA nanomicelle solution prepared in this example and the UA methanol solution is as follows figure 2 shown.

Embodiment 2

[0065] Accurately weigh 0.00456g of UA powder, dissolve it in 1ml of methanol, and dissolve it by ultrasonic to prepare a 10mM UA methanol solution; accurately weigh 0.00579g of doxorubicin hydrochloride powder, dissolve it in 1ml of secondary water, and dissolve it by ultrasonic to prepare a 10mM UA solution. Aqueous solution of doxorubicin hydrochloride; take 200 μL of UA methanol solution and add it dropwise to a beaker containing 2000 μL aqueous solution of adriamycin 0.2 μM), the concentration of UA in the solution is 1000 μM, after stirring for 8 minutes, high-speed stirring for 2 hours, drying the methanol solution to obtain carrier-free double anti-cancer nano drug UD;

[0066] The average particle size of the carrier-free double anti-cancer nano drug UD prepared in this example is about 207 nanometers, and the particle size diagram is as follows image 3 shown. The potential is around 15.1mV, and the potential is as Figure 4 shown.

[0067] The contrast imaging im...

Embodiment 3

[0069] Take the UD nano solution prepared in Example 2, add 20 μL of folic acid solution (water suspension, 10 mM) dropwise, and then sonicate for 20 min, the suspension disappears, and FUD nanoparticles with folic acid targeting are prepared.

[0070] The average particle size of the carrier-free double anti-cancer nano drug FUD prepared in this example is about 164 nanometers, and the particle size diagram is as follows Image 6 shown.

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Abstract

The invention discloses a carrier-free co-assembled tumor targeting anti-cancer nano medicine as well as a preparation method and application thereof. The carrier-free dual anti-cancer nano medicine is prepared from a hydrophobic medicine, namely ursolic acid, together with board-spectrum anti-tumor medicines such as doxorubicin in water through co-assembling, in addition, a fluorescence labeling nucleic acid aptamer, a molecular target, an antibody or polypeptide and the like with tumor targeting functions are adsorbed to the surface of the medicine through mutual electrostatic functions, then the carrier-free co-assembled tumor targeting anti-cancer nano medicine with tumor targeting and tumor microenvironment response is prepared, a synergic anti-tumor function is achieved, diagnosis and treatment integration is achieved, particularly the medicine has outstanding functions in preventing tumor transfer, and more importantly the problems that a conventional nano carrier is complex in system, indefinite in in-vivo metabolism and the like are solved.

Description

technical field [0001] The invention relates to the technical field of biomedicine, in particular to a carrier-free co-assembled tumor-targeting anti-cancer nano-medicine and a preparation method and application thereof. Background technique [0002] Cancer is one of the diseases that seriously threaten human life and health. Tumor metastasis is the main cause of death of cancer patients. According to data, the proportion of malignant tumor metastasis is as high as 60%-62.5%. Therefore, the control of tumor metastasis is the key to the prognosis of cancer patients. The key factor. To some extent, preventing tumor metastasis can control the death caused by tumor. The process of tumor metastasis involves cell shedding, infiltration, migration, implantation, angiogenesis, etc. In theory, as long as one or more of the above processes can be prevented, tumor metastasis can be inhibited. Therefore, it is urgent to find out anti-tumor metastasis drugs. [0003] Aptamers are a cl...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A61K31/704A61K31/56A61K47/54A61K47/61A61K47/68A61K47/64A61K47/69A61K9/14A61K49/00A61P35/00A61P35/04
Inventor 邵敬伟许爱笑陈思嘉郭燕
Owner FUZHOU UNIV
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