151results about How to "PH responsive" patented technology

The invention discloses a preparation method of sericin hydrogel, the method is as follows: first, weighting domestic silkworm fibroin deletion form mutation variety silkworm cocoon for LiBr or LiCl extraction and dialysis and purification to obtain a non-degradable sericin water solution with a mass percentage concentration of 0.1-4%; concentrating the sericin water solution to 1.5-10%, adding a cross-linking agent to the concentrated sericin water solution (wherein 2-500muL of the cross-linking agent is added into each L of the sericin water solution), fully mixing, and placing at 4-45 DEG C for 5 seconds to 36 hours to obtain the sericin hydrogel. The sericin hydrogel has biological activity and multiple functions, can be used as carrying growth factors, drugs and cell carriers treatment, and can be applied in repair of a variety of soft tissue injuries, including but not limited to skin damages, muscle damages, injuries of blood vessel, nerve injuries, myocardial damages, and the like, and treatment of diseases.

The invention relates to a preparing method for self-healing polysaccharide hydrogel. The preparing method comprises the steps that cellulose is added into ionic liquid to be dissolved, the mixture is then cooled to the room temperature, and a cellulose solution is obtained; the cellulose solution is heated and dripped with tert-butyl acetoacetate under the protection condition of introducing nitrogen, a reaction is carried out at the constant temperature, the solution is then cooled to the room temperature, purified and subjected to vacuum drying, and acetoacetic acid cellulose is obtained; under the room temperature, a chitosan solution is added into the acetoacetic acid cellulose solution, oscillated and mixed, and the self-healing polysaccharide hydrogel is obtained. The polysaccharide hydrogel prepared through the method has the self-healing performance, and has the pH responsiveness at the same time. The preparing method is simple in process, rich in raw material and suitable for modification of most polysaccharide derivatives; meanwhile, due to the good biocompatibility of cellulose and chitosan, the prepared polysaccharide hydrogel has good application prospects in the fields such as tissue engineering repair, medicine controlled release and biological bionics.

The invention discloses a pH (potential of hydrogen) responsive metal organic framework nanometer crystal, a preparation method and application. The pH responsive metal organic framework nanometer crystal comprises a metal organic framework and a pH responsive segment, wherein the pH responsive segment is graftedadhered to an organic ligand of the metal organic framework, and can be a pH responsive polymer, a chain segment containing a carboxylic group, or a chain segment containing a pyridine group. The pH responsive metal organic framework nanometer crystal has the advantages that the pH responsive segment is grafted onto the organic ligand of the metal organic framework, so that the contact angle of the metal organic framework is changed, namely that the hydrophilicity and hydrophobicity properties of the metal organic framework are changed, thereby increasing the interface activity of the pH responsive metal organic framework; the pH responsive segment has response property to the change of the pH value of the surrounding environment, the pH responsive metal organic framework has different hydrophilicity and hydrophobicity properties under different pH values, and then the pH responsive metal organic framework can be separated and recycled by adjusting the pH value.

The invention discloses a carrier-free co-assembled tumor targeting anti-cancer nano medicine as well as a preparation method and application thereof. The carrier-free dual anti-cancer nano medicine is prepared from a hydrophobic medicine, namely ursolic acid, together with board-spectrum anti-tumor medicines such as doxorubicin in water through co-assembling, in addition, a fluorescence labeling nucleic acid aptamer, a molecular target, an antibody or polypeptide and the like with tumor targeting functions are adsorbed to the surface of the medicine through mutual electrostatic functions, then the carrier-free co-assembled tumor targeting anti-cancer nano medicine with tumor targeting and tumor microenvironment response is prepared, a synergic anti-tumor function is achieved, diagnosis and treatment integration is achieved, particularly the medicine has outstanding functions in preventing tumor transfer, and more importantly the problems that a conventional nano carrier is complex in system, indefinite in in-vivo metabolism and the like are solved.

The invention discloses pH-responsive bi-crosslinked mussel bionic adhesive intelligent drug-loading hydrogel as well as a preparation method and application thereof, and belongs to the technical field of medical materials. The preparation method of the pH-responsive adhesive intelligent drug-loading hydrogel comprises the following steps: oxidizing a polymer containing ortho-hydroxyl into a polymer containing an aldehyde group; carrying out a Schiff base reaction with molecules containing amino groups and catechol structures to prepare a functional polymer of which the side chain contains catechol structure micromolecules; a solution of the functional polymer and the antibacterial nano material is mixed with a solution of a polymer containing amino groups and a proper amount of an oxidizing agent, so that the double-crosslinking adhesive drug-loaded hydrogel can be rapidly prepared. By adding hydrophilic drugs or drug-loaded nano particles and the like, application of the drug-loaded nano particles in biomedicine is endowed. The drug-loaded hydrogel is simple in preparation process, has good adhesion performance, can quickly release drugs through pH response, can be naturally dissociated after pH response, does not cause damage to tissues, and has the advantage of good biocompatibility.

The invention discloses a biodegradable hydrogel and method for preparing same which consists of, preparing microporous konjak gluglucosan aqueous gel, preparing refined konjaku flour, dissolving in distilled water, agitating, charging inorganic solvent, adjusting pH, carrying out crossbridging under a controlled temperature and time, water scrubbing the product yield, repeated frost thawing, freeze-drying, then swelling the konjak gluglucosan gel into the aqueous solution of acroleic acid, cross linking agent, and initiating agent, carrying out polymerization under a controlled temperature and time, and water scrubbing the obtained outcome yield.

The invention provides a bispyrene-based pH (Potential of Hydrogen)-response self-assembly polypeptide nano material, and a preparation method and application thereof. The bispyrene-based pH-response self-assembly polypeptide nano material has a structure as shown in formulas I to IV. The bispyrene-based pH-response self-assembly polypeptide nano material has pH response, can be self-assembled into nano fiber under the environment that the pH is greater than or equal to 1.0 and smaller than 8.0 and can be assembled into nanospheres under the environment that the pH is greater than or equal to 8.0, and a hydrophobic micromolecular imaging agent and medicine can be recruited, so that the utilization rate of the micromolecular imaging agent and treatment medicine can be greatly increased; the bispyrene-based pH-response self-assembly polypeptide nano material can be converted into fiber in positions of ill tissues or cells in situ, is capable of greatly prolonging the retention time, can be used for biological imaging and treating, is capable of providing a reliable diagnosis and targeted therapy method for disease treatment, and has a wide application prospect.

The invention discloses a preparation method of a pH-responsive graphene / polymer nanometer hybrid material. The preparation method comprises the following steps: under the protection of inert gas of argon or nitrogen, carrying out an esterification reaction by using a reversible addition-breakage chain transfer polymerization chain transfer agent of hydroxyl and terminal carboxyl on the surface of graphene oxide to obtain a macromolecular chain transfer agent for reversible addition-breakage chain transfer polymerization; and then, carrying out reversible addition-breakage chain transfer polymerization under initiation of azodiisobutyronitrile to obtain the pH-responsive grapheme / polymethylacrylic acid-N,N-lignocaine ethyl ester nanometer hybrid material. The graphene oxide / polymer nanometer hybrid material not only ensures that the dispersibility of graphene in water is greatly improved but also has pH responsiveness, and can be widely applied to the fields of drug controlled release carriers, biosensors, intelligent nanometer materials and the like. The preparation method is simple and easy, and industrial production of materials can be realized, so that the method has good popularization and application values.

The invention belongs to the fields of intelligent bionic materials, high-molecular materials and biomedical engineering, and concretely relates to a polydopamine particles-doped star-shaped polymer of a multi-responsive self-healing hydrogel, and the self-healing hydrogel and a preparation method thereof. In the present invention, the polydopamine nanoparticles and the star-shaped polymer SPDMAEMA-AA are prepared first; and the polydopamine nanoparticles, SPDMAEMA-AA and branched polyethyleneimine are mixed in proportion to form a crosslinked network by forming a dynamic reversible enamine bond through the acetoacetate on SPDMAEMA-AA and an amino group in the PEI molecules to obtain the hydrogel. The self-healing hydrogel material prepared by the invention has good near-infrared responsiveness, biocompatibility and high self-healing efficiency, and has potential applications in the fields of solid-state band-aids, drug slow release, cell culture, and a double-layer drive.

The invention relates to PLGA ultrasonic nano bubbles integrating ultrasonic imaging, drug delivery and gene therapy into a whole and a preparation method thereof. The preparation method comprises the following steps: preparing drug-loading nano bubbles loaded with a chemotherapy drug DOX, further modifying PLGA nano bubbles through PEI, adsorbing shRNA plasmids of MDR-1, thereby forming the composite nano bubbles. The nano bubbles have high drug loading rate and encapsulation efficiency, drug release has pH responsiveness, the loaded genes can be protected, expression of drug resistance genes MDR-1 is effectively silenced, the ultrasonic imaging capacity can be exerted, and the drugs and genes can be simultaneously and efficiently delivered to tumor locations to exert a synergistic effect.

The invention belongs to the technical field of materials and particularly relates to a method for preparing titanium-dioxide nano granules with the performance of high-efficiency ultraviolet resistance. An amphiphilic three-block polymer PEO-b-PDMA-b-PS is synthesized by an atom transfer radical polymerization (ATRP) reaction, and polymeric micelles with uniform particle-size distribution are also formed by macromolecular self-assembly. Then, the sol-gel reaction of deposited titanate occurs on the micelles in situ, organic / inorganic hybrid titanium-dioxide nano granules are obtained after drying, and then inorganic titanium-dioxide nano granules can also be obtained after further calcining. According to the method for preparing the titanium-dioxide nano granules with the performance of the high-efficiency ultraviolet resistance, the titanium-dioxide nano granules with the performance of the high-efficiency ultraviolet resistance can be prepared; and moreover, the magnitude of the particle size of the nano granules can be controlled by controlling the pH value of an environment and the polymerization degree of each chain segment of the polymer. The method has a simple preparing process and low cost and is convenient to operate, and the prepared product is safe and nontoxic, has favorable dispersing performance in water and can be applied to the fields of sun-screen cosmetics, catalytic degradation and the like.

The present invention relates to a kind of polyacrylic acid / polypeptide grafted copolymer in the first as shown and its preparation process and application. The preparation process includes the main steps of: the first setting polyacrylic acid, solvent and condensing agent in reactor; and the subsequent adding peptide homoplymer to react at 15-30 deg.c to obtain the target product. The copolymer of the present invention may be used as the nanometer level release controlling carrier of hydrophobic medicine. The present invention has the advantages of pH response of the copolymer, easy preparation and capacity of controlling the relative content of the hydrophilic chain segment and the hydrophobic chain segment of the copolymer precisely.

The present invention relates to preparation process of nano chitosan-glycyrrhizic acid particle. Glycyrrhizic acid has the effects of resisting viral hepatitis and chronic hepatitis and destroying HIV cell in blood vessel, but available clinically applied glycyrrhizic acid preparations have the problem of hard absorption. The present invention prepares powdered nano chitosan-glycyrrhizic acid particle through mixing chitosan dissolved in acid aqua and glycyrrhizic acid dissolved in ammonia solution, synthesis under mild condition and freeze drying. The product of the present invention may be re-dispersed in water to form nano particle and has certain targeting and delayed releasing performance. Compared with available marketed oral preparation, the present invention has obviously raised intestinal absorption and bioavailability.

The invention discloses novel pineapple peel residue cellulose-g-acrylic acid-kaolin-cuttlefish ink hydrogel and a preparation method and application thereof. The preparation method comprises the steps that pineapple peel residues are taken as raw materials to be cleaned, dried, smashed, bleached and treated through alkali liquor, and then pineapple peel residue cellulose is obtained; the pineapple peel residue cellulose is stirred to be dissolved in ionic liquid through heating, and grafting modification is conducted by taking ammonium persulfate as an initiator, taking N,N-methylene bisacrylamide as a cross-linking agent and taking acrylic acid as a monomer; kaolin and cuttlefish ink are added, after reacting is completed, a product is cooled to room temperature and soaked and washed by adding distilled water, freeze drying is conducted, and then the hydrogel is obtained. According to the novel pineapple peel residue cellulose-g-acrylic acid-kaolin-cuttlefish ink hydrogel, the adopted raw materials are rich in source, convenient and easy to obtain and good in economy; the technology that the pineapple peel residue cellulose is modified and then prepared into the hydrogel is simple and short in reaction time, the thermal stability of the hydrogel can be improved through the cuttlefish ink and the kaolin, and the obtained hydrogel has a good application prospect in the fields of waste water treatment and adsorption materials.

An amphiphilic fluoride segmented copolymer and its production are disclosed. The procedure is carried out by oxygen anion initiation polymerizing, reacting polymer with hydroxyl terminal group with strong lye to obtain low-polymer oxygen anion, and reacting it with methyl-acrylic acid and methyl-acrylic fluoride ester separately as initiator to obtain final product. It has fast reactive speed, higher conversion rate and purity.

The invention discloses application of an ursolic acid conjugate having the anticancer activity and serving as a drug carrier or a molecular probe carrier. The ursolic acid conjugate is characterized by being a conjugate formed by connecting of carboxy groups of ursolic acid or derivatives of ursolic acid with amino groups of guanidine drugs in the form of amido bonds. According to the specific application, a drug or a molecular probe is encapsulated and carried with the ursolic acid conjugate to form nanoparticles. When serving as the carrier, the referred conjugate has a certain anticancer effect without too high cytotoxicity and is easy and convenient to synthesize; the nanoparticles are self-assembled through a solvent exchange method, when the ursolic acid conjugate serves as the carrier of an anticancer drug or a fluorescent material, the problem on water solubility of a series of anticancer drugs is effectively solved.

The invention discloses polymer hydrogel based on Fe3+-dopamine modification and a preparing method thereof. Polymer containing a hydroxyl group is used as a main raw material, dopamine is selected as a functional group, and p-nitrophenyl chlorate is used as an activating agent and directly reacts with dopamine hydrochloride to prepare the polymer with the terminal modified by dopamine; coordination of the polymer with the terminal modified by dopamine and Fe3+ through pH adjustment is achieved. The hydrogel has adhesiveness, thermosensitivity, pH responsiveness, self-healing and other multifunctional characteristics and has potential application value in biomedical engineering and underwater mechanical coatings.

The invention discloses a composite condensation product with a double-embedding function as well as a preparation method and application of the composite condensation product. The preparation methodcomprises the following steps: (1) adding Zein into an ethanol water solution, and carrying out stirring for dissolving to obtain a Zein solution; (2) adding chitosan into an acetic acid solution, carrying out stirring for dissolving, and then carrying out overnight hydration to obtain a chitosan anti-solvent solution; (3) adding curcumin and quercetin into the Zein solution, carrying out uniformstirring and mixing, and then carrying out overnight hydration to obtain a Zein-Cur-Que mixed solution; and (4) adding the chitosan anti-solvent solution into the Zein-Cur-Que mixed solution, carryingout uniform stirring and mixing, then carrying out rotary heating to remove ethanol, and then adjusting the pH value to 3.0-7.0 to obtain the composite condensation product with the double-embeddingfunction. The composite condensation product disclosed by the invention can simultaneously embed two substances with different polarities, is high in embedding rate and high in bioavailability, and can be used in the field of food processing.

The invention discloses application of adopting an aspirin conjugate with the antitumor activity as a drug carrier or a molecular probe carrier. The aspirin conjugate is the conjugate of aspirin and a hydroxyl-containing pentacyclic triterpenoid or amino-containing anti-cancer drugs. According to the application, nanoparticles are formed for an entrapment drug or molecular probe of the aspirin conjugate. The problem of water solubility of multiple drugs is solved, the conjugate has the antitumor activity and does not have large cytotoxicity when as the carrier, the entrapment drug can achieve the better curative effect, and the entrapment molecular probe can achieve integration of diagnosis and treatment.

The invention discloses a pH-responsive controlled release pesticide microcapsule suspension. The pH-responsive controlled release pesticide microcapsule suspension comprises 1-55 wt% of a capsule core effective component, 2-25 wt% of a capsule material, 0-15 wt% of a capsule core solvent, 2-6 wt% of a dispersant, 0.5-1 wt% of a defoamer, 0.5-2 wt% of a preservative, 3-5 wt% of an antifreeze agent, 0.1-2 wt% of a thickener, and the balance of water, and the capsule material is polyurethane obtained by crosslinking and curing isocyanate, a polyurethane soft segment and a carboxyl group-containing chain extender, and a weight ratio of the isocyanate to the polyurethane soft segment to the carboxyl group-containing chain extender is (10-20):(20-85):(4-30). The invention also discloses a preparation method of the pH-responsive controlled release pesticide microcapsule suspension. The pH-responsive controlled release pesticide microcapsule suspension prepared in the invention has the advantages of simple preparation process, stable quality, high efficiency, and realization of adjustment of the release rate according to different pH values.

The invention belongs to the field of interdiscipline of civil engineering materials and microbiology, and relates to a hydrogel encapsulated bacterial spore self-repairing material with pH responsiveness and a cement-based concrete self-repairing method. The self-repairing material comprises hydrogel with pH responsiveness, bacterial spores encapsulated by the hydrogel and a calcium source, wherein the hydrogel is prepared from a cross-linked hydrophilic polymer, and the bacterial spores comprise bacillus pseudofirmus spores. The hydrogel used in the invention has pH responsiveness, and can encapsulate bacterial spores on the premise of ensuring no damage to cement strength, thus successfully realizing cement self-healing.

The invention discloses a multilayer structure metal organic framework nanocrystal and a preparation method thereof. The preparation method comprises the following steps that (1) first metal organic framework crystal particles are used as an emulsifying agent; oil-in-water type pickering emulsion is prepared; cooling is performed to below the melting point of solid paraffin; first paraffin microspheres are obtained; (2) maleic anhydride or methacrylic anhydride is used for performing reaction modification on the first paraffin microspheres; second paraffin microspheres are obtained; the second paraffin microspheres fix a second metal organic framework; (3) a third metal organic framework is grown on the surface of side-chain radicals, containing carboxyls, of the second metal organic framework through heteroepitaxy, or a sulfhydryl compound is added to perform sulfydryl-alkene click reaction, so that the multilayer structure metal organic framework nanocrystal is obtained. The prepared multilayer structure metal organic framework nanocrystal has anisotropy in the surface structure and property, and can be applied to the fields of catalysis, metal surface MOFs membrane preparation, oil-water separation and the like; the application is wide.

The present invention relates to a pH-responsive drilling fluid aluminum-based anti-sloughing agent and a preparation method thereof. According to the present invention, the pH-responsive drilling fluid aluminum-based anti-sloughing agent prepared by carrying out a reaction on polyether polyol, a lactone monomer, an initiator, an aluminum compound and a pH value adjuster has pH responsiveness, stably exists in the drilling fluid (the pH value is more than 8) in the form of a dissolved state, can rapidly respond within a pH responsive range (the pH value is more than or equal to 5 and is less than or equal to 7.5) to form aluminum hydroxide precipitate, and can block stratum bore throat and micro-cracks through physical and chemical action, can improve the collapse resistance of the aluminum-based drilling fluid, can strengthen the stability of the borehole wall, and can meet the complex stratum exploration and development requirements; and the preparation method has characteristics of simpleness, easy reaction control, low production cost, stable product quality, and easy industrial application.

The invention provides TA-Fe (III) modified PLGA nanoparticles coating doxorubicin hydrochloride and a preparation method thereof. The mPEG-PLGA is selected as a carrier material, TA and Fe (III) are used as modification materials, and the nanoparticles are prepared by combination of a multiple emulsion-solvent volatilization method and coordination modification of the TA and Fe (III). The encapsulation efficiency of the nanoparticles is 80.14+ / -9.79%, and the drug loading is 0.85+ / -0.08%. The particle size of the nanoparticles is 146.9+ / -14.3 nm, the Zeta potential is -21.0+ / -0.2 mV, and the polydispersity index is 0.214+ / -0.113. The surfaces of the nanoparticles obtained by the preparation method are smooth and round without adhesion, and the nanoparticles have controllable size, small particle size and narrow range of particle size, are convenient for intravenous injection, and have good sustained release effect. The TA-Fe (III) modified nanoparticles have a good drug encapsulation effect, can significantly reduce drug burst release and have a pH responsiveness effect. Therefore, the nanoparticles have broader application prospects in targeted delivery as an anti-tumor drug.

The invention provides a polymer, a preparation method and a poly-N-isopropylacrylamide europium complex micelle, and belongs to the field of europoium complexes. The polymer is methoxypolyethylene glycol-poly-N-isopropylacrylamide-polymethylacrylic acid N,N-diethylaminoethyl, the structural formula is shown as the formula I, and the abbreviate formula is mPEG23-PNIPAM-PDEA, wherein mPEG23 is methoxypolyethylene glycol, PNIPAM is poly-N-isopropylacrylamide, and PDEA is polymethylacrylic acid N,N-diethylaminoethyl. The structural formula of the europium complex micelle is shown as the formula II. Due to the fact that the polymer (mPEG23-PNIPAM-PDEA) has good thermosensitivity and pH sensibility, the polymer can be used as a chemical conveying carrier after being prepared into the europium complex micelle.

The invention discloses a preparation method of a fluorinated polyacrylic copolymer antibacterial composite material and a obtained product and application thereof. A fluorine-free acrylic monomer andA fluorine-containing acrylate monomer are polymerized to form a fluorine-containing polyacrylic copolymer, and then the fluorine-containing polyacrylic copolymer is appropriately cross-linked to form a copolymer with a certain degree of crosslinking. Finally, the crosslinked copolymer is compounded with an antibacterial agent to form a composite antibacterial material. The preparation method ofthe invention has relatively mild reaction conditions, simple operation, and high sample yield. The obtained antibacterial composite material has the characteristics of antibacterial adhesion, sterilization, pH responsiveness, and fluorescence. The product can be made into a film or coating use on the surface of a product, not only can prevent adhesion of bacteria, dust and the like, but also canefficiently kill the bacteria attached thereon, prevent the reduction of bactericidal efficiency caused by the adhesion of dead bacteria or live bacteria. The product has good antifouling and antibacterial effects, and has good application prospects in the fields of medicine, biology, health, food and the like.

The present invention provides an antibacterial peptide, and a pharmaceutical composition and applications thereof. The antibacterial peptide comprises any one of an antibacterial peptide 1, an antibacterial peptide 2 and an antibacterial peptide 3, the amino acid sequence of the antibacterial peptide 1 is represented by SEQ ID No: 1, the amino acid sequence of the antibacterial peptide 2 is represented by SEQ ID No: 2, and the amino acid sequence of the antibacterial peptide 3 is represented by SEQ ID No: 3. The three antibacterial peptides are formed by modifying natural antibacterial peptides PGLa-AM1 as the basis, and have the characteristics of stronger antibacterial effect and better performance. The inhibitory effect of the three antibacterial peptides on helicobacter pylori is stronger than that of PGLa-AM1, the broad-spectrum antibacterial effect of the antibacterial peptide 3 is the strongest, and the antibacterial peptide 3 has good pH responsiveness, so that the antibacterial peptide 3 is in better contact with helicobacter pylori and inhibits growth of helicobacter pylori. The three antibacterial peptides have advantages of strong antibacterial activity, strong biocompatibility and low cost, and are expected to be widely used.

The invention belongs to the technical field of preparation of greenhouse covering film materials and system design of the greenhouse covering film materials, and particularly discloses a greenhouse covering film material easy to clean, a preparation method and a cleaning method. An outer layer of a greenhouse covering film easy to clean is composed of a polyphenol-metal nano-particle thin film, has good optical transmittance, and does not affect the optical performance of an original greenhouse covering film body film; and the thin film is small in mass and good in biocompatibility, does not corrode or damage the surfaces of a greenhouse covering film and a greenhouse covering glass body, and can be used on a large scale. The prepared polyphenol-metal nano-particle thin film can prevent dust from being directly deposited on the surfaces of the greenhouse covering film and greenhouse covering glass; and when the light transmittance of the greenhouse covering film becomes poor due to the dust deposition amount, the nano-particle thin film formed by plant polyphenol and metal ions can be dissociated by spraying a cleaning agent and washing with clear water, and meanwhile, the dust adhered to the nano-particle thin film is removed together, so that the greenhouse covering film is easy to clean.

The invention relates to a double-crosslinked Pickering emulsion and a preparation method and application thereof, and belongs to the field of modern healthy food processing. The invention provides apreparation method of a double-crosslinked Pickering emulsion. The preparation method of the double-crosslinked Pickering emulsion comprises a preparation method of a Pickering emulsion of gliadin-sodium alginate composite particles and a Pickering emulsion formed by crosslinking calcium ions and glutamine transaminase. The method comprises the following steps: preparing a gliadin-curcumin mixed solution; preparing a sodium alginate stock solution; preparing a curcumin-loaded gliadin suspension; and acquiring the Pickering emulsion of the gliadin-sodium alginate composite particles or the Pickering emulsion formed by crosslinking the calcium ions and the glutamine transaminase. According to the invention, the curcumin-loaded composite particles are constructed by utilizing gliadin and sodium alginate for the first time to form the stable Pickering emulsion, and the Pickering emulsion can be used for embedding food nutrient substances.

The invention discloses a preparation method of injectable bi-crosslinking hydrogel dynamically combined with natural polyphenol, and the hydrogel material comprises phenylboronic acid group and vinyl group modified hyaluronic acid, vinyl group modified gelatin, a photoinitiator and natural polyphenol. The preparation method comprises the following steps: dissolving the modified hyaluronic acid, the modified gelatin and the photoinitiator in a phosphate buffer solution, uniformly mixing with a phosphate buffer solution containing natural polyphenol to obtain mono-crosslinked hydrogel, and then irradiating with ultraviolet light to obtain the bi-crosslinked hydrogel. The preparation process is simple and easy to operate, and preparation conditions are mild. The hydrogel can simulate natural nucleus pulposus tissues, has good mechanical properties, injectability, adhesion, pH responsiveness, biocompatibility, oxidation resistance and anti-inflammatory performance, provides a new design idea for intervertebral disc tissue engineering materials, and also has good application prospects in other biomedical fields.