151results about How to "PH responsive" patented technology

The invention relates to a preparing method for self-healing polysaccharide hydrogel. The preparing method comprises the steps that cellulose is added into ionic liquid to be dissolved, the mixture is then cooled to the room temperature, and a cellulose solution is obtained; the cellulose solution is heated and dripped with tert-butyl acetoacetate under the protection condition of introducing nitrogen, a reaction is carried out at the constant temperature, the solution is then cooled to the room temperature, purified and subjected to vacuum drying, and acetoacetic acid cellulose is obtained; under the room temperature, a chitosan solution is added into the acetoacetic acid cellulose solution, oscillated and mixed, and the self-healing polysaccharide hydrogel is obtained. The polysaccharide hydrogel prepared through the method has the self-healing performance, and has the pH responsiveness at the same time. The preparing method is simple in process, rich in raw material and suitable for modification of most polysaccharide derivatives; meanwhile, due to the good biocompatibility of cellulose and chitosan, the prepared polysaccharide hydrogel has good application prospects in the fields such as tissue engineering repair, medicine controlled release and biological bionics.

The invention discloses a pH (potential of hydrogen) responsive metal organic framework nanometer crystal, a preparation method and application. The pH responsive metal organic framework nanometer crystal comprises a metal organic framework and a pH responsive segment, wherein the pH responsive segment is graftedadhered to an organic ligand of the metal organic framework, and can be a pH responsive polymer, a chain segment containing a carboxylic group, or a chain segment containing a pyridine group. The pH responsive metal organic framework nanometer crystal has the advantages that the pH responsive segment is grafted onto the organic ligand of the metal organic framework, so that the contact angle of the metal organic framework is changed, namely that the hydrophilicity and hydrophobicity properties of the metal organic framework are changed, thereby increasing the interface activity of the pH responsive metal organic framework; the pH responsive segment has response property to the change of the pH value of the surrounding environment, the pH responsive metal organic framework has different hydrophilicity and hydrophobicity properties under different pH values, and then the pH responsive metal organic framework can be separated and recycled by adjusting the pH value.

The invention discloses a carrier-free co-assembled tumor targeting anti-cancer nano medicine as well as a preparation method and application thereof. The carrier-free dual anti-cancer nano medicine is prepared from a hydrophobic medicine, namely ursolic acid, together with board-spectrum anti-tumor medicines such as doxorubicin in water through co-assembling, in addition, a fluorescence labeling nucleic acid aptamer, a molecular target, an antibody or polypeptide and the like with tumor targeting functions are adsorbed to the surface of the medicine through mutual electrostatic functions, then the carrier-free co-assembled tumor targeting anti-cancer nano medicine with tumor targeting and tumor microenvironment response is prepared, a synergic anti-tumor function is achieved, diagnosis and treatment integration is achieved, particularly the medicine has outstanding functions in preventing tumor transfer, and more importantly the problems that a conventional nano carrier is complex in system, indefinite in in-vivo metabolism and the like are solved.

The invention discloses pH-responsive bi-crosslinked mussel bionic adhesive intelligent drug-loading hydrogel as well as a preparation method and application thereof, and belongs to the technical field of medical materials. The preparation method of the pH-responsive adhesive intelligent drug-loading hydrogel comprises the following steps: oxidizing a polymer containing ortho-hydroxyl into a polymer containing an aldehyde group; carrying out a Schiff base reaction with molecules containing amino groups and catechol structures to prepare a functional polymer of which the side chain contains catechol structure micromolecules; a solution of the functional polymer and the antibacterial nano material is mixed with a solution of a polymer containing amino groups and a proper amount of an oxidizing agent, so that the double-crosslinking adhesive drug-loaded hydrogel can be rapidly prepared. By adding hydrophilic drugs or drug-loaded nano particles and the like, application of the drug-loaded nano particles in biomedicine is endowed. The drug-loaded hydrogel is simple in preparation process, has good adhesion performance, can quickly release drugs through pH response, can be naturally dissociated after pH response, does not cause damage to tissues, and has the advantage of good biocompatibility.

The invention discloses a biodegradable hydrogel and method for preparing same which consists of, preparing microporous konjak gluglucosan aqueous gel, preparing refined konjaku flour, dissolving in distilled water, agitating, charging inorganic solvent, adjusting pH, carrying out crossbridging under a controlled temperature and time, water scrubbing the product yield, repeated frost thawing, freeze-drying, then swelling the konjak gluglucosan gel into the aqueous solution of acroleic acid, cross linking agent, and initiating agent, carrying out polymerization under a controlled temperature and time, and water scrubbing the obtained outcome yield.

The invention provides a bispyrene-based pH (Potential of Hydrogen)-response self-assembly polypeptide nano material, and a preparation method and application thereof. The bispyrene-based pH-response self-assembly polypeptide nano material has a structure as shown in formulas I to IV. The bispyrene-based pH-response self-assembly polypeptide nano material has pH response, can be self-assembled into nano fiber under the environment that the pH is greater than or equal to 1.0 and smaller than 8.0 and can be assembled into nanospheres under the environment that the pH is greater than or equal to 8.0, and a hydrophobic micromolecular imaging agent and medicine can be recruited, so that the utilization rate of the micromolecular imaging agent and treatment medicine can be greatly increased; the bispyrene-based pH-response self-assembly polypeptide nano material can be converted into fiber in positions of ill tissues or cells in situ, is capable of greatly prolonging the retention time, can be used for biological imaging and treating, is capable of providing a reliable diagnosis and targeted therapy method for disease treatment, and has a wide application prospect.

The invention discloses a preparation method of a pH-responsive graphene /polymer nanometer hybrid material. The preparation method comprises the following steps: under the protection of inert gas of argon or nitrogen, carrying out an esterification reaction by using a reversible addition-breakage chain transfer polymerization chain transfer agent of hydroxyl and terminal carboxyl on the surface of graphene oxide to obtain a macromolecular chain transfer agent for reversible addition-breakage chain transfer polymerization; and then, carrying out reversible addition-breakage chain transfer polymerization under initiation of azodiisobutyronitrile to obtain the pH-responsive grapheme/polymethylacrylic acid-N,N-lignocaine ethyl ester nanometer hybrid material. The graphene oxide/polymer nanometer hybrid material not only ensures that the dispersibility of graphene in water is greatly improved but also has pH responsiveness, and can be widely applied to the fields of drug controlled release carriers, biosensors, intelligent nanometer materials and the like. The preparation method is simple and easy, and industrial production of materials can be realized, so that the method has good popularization and application values.

The invention relates to PLGA ultrasonic nano bubbles integrating ultrasonic imaging, drug delivery and gene therapy into a whole and a preparation method thereof. The preparation method comprises the following steps: preparing drug-loading nano bubbles loaded with a chemotherapy drug DOX, further modifying PLGA nano bubbles through PEI, adsorbing shRNA plasmids of MDR-1, thereby forming the composite nano bubbles. The nano bubbles have high drug loading rate and encapsulation efficiency, drug release has pH responsiveness, the loaded genes can be protected, expression of drug resistance genes MDR-1 is effectively silenced, the ultrasonic imaging capacity can be exerted, and the drugs and genes can be simultaneously and efficiently delivered to tumor locations to exert a synergistic effect.

The present invention relates to a kind of polyacrylic acid/polypeptide grafted copolymer in the first as shown and its preparation process and application. The preparation process includes the main steps of: the first setting polyacrylic acid, solvent and condensing agent in reactor; and the subsequent adding peptide homoplymer to react at 15-30 deg.c to obtain the target product. The copolymer of the present invention may be used as the nanometer level release controlling carrier of hydrophobic medicine. The present invention has the advantages of pH response of the copolymer, easy preparation and capacity of controlling the relative content of the hydrophilic chain segment and the hydrophobic chain segment of the copolymer precisely.

The present invention relates to preparation process of nano chitosan-glycyrrhizic acid particle. Glycyrrhizic acid has the effects of resisting viral hepatitis and chronic hepatitis and destroying HIV cell in blood vessel, but available clinically applied glycyrrhizic acid preparations have the problem of hard absorption. The present invention prepares powdered nano chitosan-glycyrrhizic acid particle through mixing chitosan dissolved in acid aqua and glycyrrhizic acid dissolved in ammonia solution, synthesis under mild condition and freeze drying. The product of the present invention may be re-dispersed in water to form nano particle and has certain targeting and delayed releasing performance. Compared with available marketed oral preparation, the present invention has obviously raised intestinal absorption and bioavailability.

An amphiphilic fluoride segmented copolymer and its production are disclosed. The procedure is carried out by oxygen anion initiation polymerizing, reacting polymer with hydroxyl terminal group with strong lye to obtain low-polymer oxygen anion, and reacting it with methyl-acrylic acid and methyl-acrylic fluoride ester separately as initiator to obtain final product. It has fast reactive speed, higher conversion rate and purity.

The invention discloses a composite condensation product with a double-embedding function as well as a preparation method and application of the composite condensation product. The preparation methodcomprises the following steps: (1) adding Zein into an ethanol water solution, and carrying out stirring for dissolving to obtain a Zein solution; (2) adding chitosan into an acetic acid solution, carrying out stirring for dissolving, and then carrying out overnight hydration to obtain a chitosan anti-solvent solution; (3) adding curcumin and quercetin into the Zein solution, carrying out uniformstirring and mixing, and then carrying out overnight hydration to obtain a Zein-Cur-Que mixed solution; and (4) adding the chitosan anti-solvent solution into the Zein-Cur-Que mixed solution, carryingout uniform stirring and mixing, then carrying out rotary heating to remove ethanol, and then adjusting the pH value to 3.0-7.0 to obtain the composite condensation product with the double-embeddingfunction. The composite condensation product disclosed by the invention can simultaneously embed two substances with different polarities, is high in embedding rate and high in bioavailability, and can be used in the field of food processing.

The invention discloses a pH-responsive controlled release pesticide microcapsule suspension. The pH-responsive controlled release pesticide microcapsule suspension comprises 1-55 wt% of a capsule core effective component, 2-25 wt% of a capsule material, 0-15 wt% of a capsule core solvent, 2-6 wt% of a dispersant, 0.5-1 wt% of a defoamer, 0.5-2 wt% of a preservative, 3-5 wt% of an antifreeze agent, 0.1-2 wt% of a thickener, and the balance of water, and the capsule material is polyurethane obtained by crosslinking and curing isocyanate, a polyurethane soft segment and a carboxyl group-containing chain extender, and a weight ratio of the isocyanate to the polyurethane soft segment to the carboxyl group-containing chain extender is (10-20):(20-85):(4-30). The invention also discloses a preparation method of the pH-responsive controlled release pesticide microcapsule suspension. The pH-responsive controlled release pesticide microcapsule suspension prepared in the invention has the advantages of simple preparation process, stable quality, high efficiency, and realization of adjustment of the release rate according to different pH values.

The invention belongs to the field of interdiscipline of civil engineering materials and microbiology, and relates to a hydrogel encapsulated bacterial spore self-repairing material with pH responsiveness and a cement-based concrete self-repairing method. The self-repairing material comprises hydrogel with pH responsiveness, bacterial spores encapsulated by the hydrogel and a calcium source, wherein the hydrogel is prepared from a cross-linked hydrophilic polymer, and the bacterial spores comprise bacillus pseudofirmus spores. The hydrogel used in the invention has pH responsiveness, and can encapsulate bacterial spores on the premise of ensuring no damage to cement strength, thus successfully realizing cement self-healing.

The invention provides TA-Fe (III) modified PLGA nanoparticles coating doxorubicin hydrochloride and a preparation method thereof. The mPEG-PLGA is selected as a carrier material, TA and Fe (III) are used as modification materials, and the nanoparticles are prepared by combination of a multiple emulsion-solvent volatilization method and coordination modification of the TA and Fe (III). The encapsulation efficiency of the nanoparticles is 80.14+/-9.79%, and the drug loading is 0.85+/-0.08%. The particle size of the nanoparticles is 146.9+/-14.3 nm, the Zeta potential is -21.0+/-0.2 mV, and the polydispersity index is 0.214+/-0.113. The surfaces of the nanoparticles obtained by the preparation method are smooth and round without adhesion, and the nanoparticles have controllable size, small particle size and narrow range of particle size, are convenient for intravenous injection, and have good sustained release effect. The TA-Fe (III) modified nanoparticles have a good drug encapsulation effect, can significantly reduce drug burst release and have a pH responsiveness effect. Therefore, the nanoparticles have broader application prospects in targeted delivery as an anti-tumor drug.

The invention discloses a preparation method of a fluorinated polyacrylic copolymer antibacterial composite material and a obtained product and application thereof. A fluorine-free acrylic monomer andA fluorine-containing acrylate monomer are polymerized to form a fluorine-containing polyacrylic copolymer, and then the fluorine-containing polyacrylic copolymer is appropriately cross-linked to form a copolymer with a certain degree of crosslinking. Finally, the crosslinked copolymer is compounded with an antibacterial agent to form a composite antibacterial material. The preparation method ofthe invention has relatively mild reaction conditions, simple operation, and high sample yield. The obtained antibacterial composite material has the characteristics of antibacterial adhesion, sterilization, pH responsiveness, and fluorescence. The product can be made into a film or coating use on the surface of a product, not only can prevent adhesion of bacteria, dust and the like, but also canefficiently kill the bacteria attached thereon, prevent the reduction of bactericidal efficiency caused by the adhesion of dead bacteria or live bacteria. The product has good antifouling and antibacterial effects, and has good application prospects in the fields of medicine, biology, health, food and the like.

The present invention provides an antibacterial peptide, and a pharmaceutical composition and applications thereof. The antibacterial peptide comprises any one of an antibacterial peptide 1, an antibacterial peptide 2 and an antibacterial peptide 3, the amino acid sequence of the antibacterial peptide 1 is represented by SEQ ID No: 1, the amino acid sequence of the antibacterial peptide 2 is represented by SEQ ID No: 2, and the amino acid sequence of the antibacterial peptide 3 is represented by SEQ ID No: 3. The three antibacterial peptides are formed by modifying natural antibacterial peptides PGLa-AM1 as the basis, and have the characteristics of stronger antibacterial effect and better performance. The inhibitory effect of the three antibacterial peptides on helicobacter pylori is stronger than that of PGLa-AM1, the broad-spectrum antibacterial effect of the antibacterial peptide 3 is the strongest, and the antibacterial peptide 3 has good pH responsiveness, so that the antibacterial peptide 3 is in better contact with helicobacter pylori and inhibits growth of helicobacter pylori. The three antibacterial peptides have advantages of strong antibacterial activity, strong biocompatibility and low cost, and are expected to be widely used.

The invention belongs to the technical field of preparation of greenhouse covering film materials and system design of the greenhouse covering film materials, and particularly discloses a greenhouse covering film material easy to clean, a preparation method and a cleaning method. An outer layer of a greenhouse covering film easy to clean is composed of a polyphenol-metal nano-particle thin film, has good optical transmittance, and does not affect the optical performance of an original greenhouse covering film body film; and the thin film is small in mass and good in biocompatibility, does not corrode or damage the surfaces of a greenhouse covering film and a greenhouse covering glass body, and can be used on a large scale. The prepared polyphenol-metal nano-particle thin film can prevent dust from being directly deposited on the surfaces of the greenhouse covering film and greenhouse covering glass; and when the light transmittance of the greenhouse covering film becomes poor due to the dust deposition amount, the nano-particle thin film formed by plant polyphenol and metal ions can be dissociated by spraying a cleaning agent and washing with clear water, and meanwhile, the dust adhered to the nano-particle thin film is removed together, so that the greenhouse covering film is easy to clean.

The invention relates to a double-crosslinked Pickering emulsion and a preparation method and application thereof, and belongs to the field of modern healthy food processing. The invention provides apreparation method of a double-crosslinked Pickering emulsion. The preparation method of the double-crosslinked Pickering emulsion comprises a preparation method of a Pickering emulsion of gliadin-sodium alginate composite particles and a Pickering emulsion formed by crosslinking calcium ions and glutamine transaminase. The method comprises the following steps: preparing a gliadin-curcumin mixed solution; preparing a sodium alginate stock solution; preparing a curcumin-loaded gliadin suspension; and acquiring the Pickering emulsion of the gliadin-sodium alginate composite particles or the Pickering emulsion formed by crosslinking the calcium ions and the glutamine transaminase. According to the invention, the curcumin-loaded composite particles are constructed by utilizing gliadin and sodium alginate for the first time to form the stable Pickering emulsion, and the Pickering emulsion can be used for embedding food nutrient substances.

The invention discloses a preparation method of injectable bi-crosslinking hydrogel dynamically combined with natural polyphenol, and the hydrogel material comprises phenylboronic acid group and vinyl group modified hyaluronic acid, vinyl group modified gelatin, a photoinitiator and natural polyphenol. The preparation method comprises the following steps: dissolving the modified hyaluronic acid, the modified gelatin and the photoinitiator in a phosphate buffer solution, uniformly mixing with a phosphate buffer solution containing natural polyphenol to obtain mono-crosslinked hydrogel, and then irradiating with ultraviolet light to obtain the bi-crosslinked hydrogel. The preparation process is simple and easy to operate, and preparation conditions are mild. The hydrogel can simulate natural nucleus pulposus tissues, has good mechanical properties, injectability, adhesion, pH responsiveness, biocompatibility, oxidation resistance and anti-inflammatory performance, provides a new design idea for intervertebral disc tissue engineering materials, and also has good application prospects in other biomedical fields.