90results about How to "Realize the integration of diagnosis and treatment" patented technology

The invention relates to a polydopamine coated gold core/hollow silica shell nanometer material as well as preparation and application thereof. The nanometer material is prepared according to the following steps: coating gold nanoparticles and perfluorohexane (PFH) in the inner cavity of hollow mesoporous silica, and coating polydopamine on the surface of hollow mesoporous silica; stably reducingHAuCl4 with sodium citrate so as to obtain gold nanoparticles, taking ethyl orthosilicate as a silicon source, forming mesoporous solid silicon-coated gold nanoparticles in a mixed solution of ethanol, ultrapure water and ammonium hydroxide, and etching gold core/hollow silica shell nanoparticles with sodium carbonate; modifying amino on the surface of the nanoparticles, and coating perfluorohexane; finally, coating a polydopamine layer on the surface of the nanoparticles, thereby obtaining the product. The nano platform prepared by the method disclosed by the invention has good stability andexcellent biocompatibility, also has good US/CT/PA imaging and photothermal therapy effects, provides a novel method for development of the multimode imaging contrast agent and diagnosis and treatmentintegrated platform, and is wide in application prospects.

The invention relates to a preparation method of hollow mesoporous silica loaded by nanometer star-shaped gold particles. The method includes the steps of forming solid silicon wrapped by meso-porous silicon in an ethyl alcohol, ultrapure water and ammonia mixed solution with ethyl orthosilicate as a silicon source, etching the solid silicon through sodium carbonate to form hollow mesoporous silica, modifying the surface of the hollow mesoporous silica by sulfydryl and loading the surface of the hollow mesoporous silica with nanometer gold particles so that the hollow mesoporous silica can serve as seeds, making the seeds grow into the hollow mesoporous silica loaded by nanometer star-shaped gold particles in a chloroauric acid solution, and wrapping perfluorohexane and modifying polyethylene glycol with one end being -SH. The prepared nanometer particles have excellent biocompatibility and long in-vivo blood circulation time, have good US/CT/PA imaging and photo-thermal treatment effects and are wide in application prospects, and a new method is provided for development of multi-mode imaging contrast media and treatment integrated platforms.

The invention discloses a carrier-free co-assembled tumor targeting anti-cancer nano medicine as well as a preparation method and application thereof. The carrier-free dual anti-cancer nano medicine is prepared from a hydrophobic medicine, namely ursolic acid, together with board-spectrum anti-tumor medicines such as doxorubicin in water through co-assembling, in addition, a fluorescence labeling nucleic acid aptamer, a molecular target, an antibody or polypeptide and the like with tumor targeting functions are adsorbed to the surface of the medicine through mutual electrostatic functions, then the carrier-free co-assembled tumor targeting anti-cancer nano medicine with tumor targeting and tumor microenvironment response is prepared, a synergic anti-tumor function is achieved, diagnosis and treatment integration is achieved, particularly the medicine has outstanding functions in preventing tumor transfer, and more importantly the problems that a conventional nano carrier is complex in system, indefinite in in-vivo metabolism and the like are solved.

The invention relates to a MRI (magnetic resonance imaging)-guided targeted photo-thermal agent and a preparation method of a chemotherapeutic system of the MRI-guided targeted photo-thermal agent. The method comprises the following steps: by using a hydrothermal method, preparing superparamagnetic ferroferric oxide nano-particles on PEI modified MoS2 in an in-situ preparation mode, thus obtaining a PEI modified MoS2 nano composite; preparing polyethylene glycol coupled targeting molecules by using EDC chemical; by using an EDC chemical, connecting polyethylene glycol with the targeting molecules to the PEI modified MoS2 nano composite in a covalent linkage mode; and through physical adsorption, loading a micromolecular anti-tumor drug on a carrier. The method disclosed by the invention is easy to operate and simple in step, and the prepared multi-functional MoS2 nano composite is good in biological compatibility, can be specifically gathered at tumor sites so as to effectively kill tumor cells, and can effectively integrate early diagnosis and late treatment so as to realize the integration of diagnosis and treatment of cancer treatment.

The invention discloses amphiphilic segmented copolymers, nanoparticles containing the amphiphilic segmented copolymers, a preparation method of the amphiphilic segmented copolymers and a use of the nanoparticles. The amphiphilic segmented copolymers are shown in the structural formulas (I) to (IV). In the structural formulas (I) to (IV), R1 represents H or methyl, R2, R3 and R5 represent H, alkyl or aryl, R4 represents hydroxyl or methyl, x is an integer of 3-20, Spacer is a spacer arm, the spacer arm is optional, and when the spacer arm exists, the spacer arm is one of an ester group, an alkyl group, a triazole group and a thioether group. The nanoparticle obtained by chelating of the amphiphilic segmented copolymer and rare earth ions has good biocompatibility, has excellent fluorescence imaging performances and MRI imaging performances and has a wide application value in the field of medicine and biology and especially in breast cancer diagnosis and treatment.

The invention relates to a preparation method and applications of a mesoporous calcium carbonate medicinal composition modified by hyaluronic acid, for effectively simultaneously realizing targeting, controlled release and ultrasonic therapy and ultrasonic imaging combination, and further realizing diagnosis and treatment integration on cancer. The technical scheme is as follows: the preparation method comprises the following steps: modifying hyaluronic acid on aminated calcium carbonate obtained by reacting mesoporous calcium carbonate and 3-aminopropyl triethoxysilane, and then loading a micromolecular sonosensitizer, thus obtaining the mesoporous calcium carbonate medicinal composition modified by hyaluronic acid. The preparation method has the advantages that a preparation process is simple, a method is stable and reliable, the production cost is low, and the prepared mesoporous calcium carbonate modified by hyaluronic acid and the medicinal composition of mesoporous calcium carbonate simultaneously have the efficacies of tumor targeting, in vitro and in vivo double-stimulation (in acid environment and under high-intensity focused ultrasonic condition) sensitive medicine release, sonodynamic therapy and ultrasonic imaging combined diagnosis and treatment integration, belong to an innovation in tumor treatment medicines, and have enormous economic and social benefits.

The invention relates to a preparation method of a folic acid coupled gold nano-rod/polypyrrole/ferroferric oxide multifunctional composite nano diagnosis and treatment agent. The preparation method comprises the following steps: by adopting composite nanoparticles consisting of gold nano-rods, polypyrrole and ferroferric oxide as a substrate, modifying the surfaces of the composite nanoparticles by using polycationic electrolyte chitosan and polyanionic electrolyte sodium alginate respectively by virtue of a layer-by-layer self-assembly process to ensure that the surfaces of the nanoparticles contain amino groups, and then connecting with carboxy groups of folic acid. The prepared multifunctional composite nano diagnosis and treatment agent disclosed by the invention has the advantages of high photo-thermal conversion efficiency, good biocompatibility, active tumor targeting performance, strong cancer cell killing ability, significant imaging effect, a function of being controlled for a long time, and the like, and can be applied to treatment, pathological magnetic resonance imaging analysis and medical diagnosis of X-ray computer tomography and ultrasonic imaging of tumors or cancers of human bodies or other mammals.

The invention relates to a complex of a bifunctional connecting agent realizing core coordination with carbonyl metal and a preparation method thereof, and belongs to the technical field of radiopharmaceutical chemistry. The bifunctional connecting agent is N,N'-bi[2-hydroxyl-5-( propyloic) benzyl] ethylenediamine-N,N'-oxalic acid, and has a structure shown in the description, wherein at least onein R1 and R2 is a targeted small molecule or polypeptide or protein big molecule; the metal is Tc or Re. The carbonyl Tc/Re core marked HBED-CC complex is successfully obtained for the first time, different nuclide (68Ga, 99mTc/Re) marks of the same marking precursors can be realized; by aiming at the mutual supplementation of two development modes (PET/SPECT) in the same target point, the 188/186Re complex can be used for radioactive therapy medicine study. A novel idea is provided for the development of a Tc-99m single photon radioactive tracer agents and Re-188/186 radioactive treatment medicine; meanwhile, the application range of the HBED-CC derivatives as radiopharmaceutical marker precursors is also expanded.

The invention relates to a nano-enzyme modified polymer carrier and a preparation method and antitumor nanoparticles thereof. The preparation method of the nano-enzyme modified polymer carrier comprises the following steps of enabling 4-phenylboronic acid pinacol ester to dissolve in dimethyl sulfoxide, and adding an activator to obtain activated liquid; enabling polyethyleneimine to dissolve in water, adding the activated liquid, and performing stirring for the first time to obtain a first intermediate product; loading nano-enzymes on polysaccharide to obtain a second intermediate product; and adding the first intermediate product in the second intermediate product, and performing stirring for the second time to obtain the nano-enzyme modified polymer carrier. The polymer carrier preparedby the method can adsorb a functional molecule bearing negative charges, through self-assembly, stable nanoparticles are formed, under the condition that tumor tissues are high in H2O2 content and acid environment exists, specific release of medicine functional molecules in target positions can be realized, and besides, oxygen can be generated under the condition that the H2O2 content is higher and the acid environment exists. The hypoxia can be improved, and the drug tolerance can be reduced.

The invention relates to a carbon dot used as an antitumor drug and a preparing method and application of the carbon dot. With arginine being a carbon source, arginine is completely dissolved with water, and an appropriate amount of thylenediamine is added and subjected to ultrasonic dissolving and microwave heating to obtain the target product carbon dot. Since cancer cells themselves in an organism contain hydrogen peroxide, the carbon dot can be used for treating cancer. The carbon dot can not only conduct fluorescence imaging, but also has the advantages of being high in biocompatibility,large in NO releasing amount, long in acting circular time, high in drug stability, cheap, easy to obtain and the like.

The invention discloses a glycyrrhetinic acid-modified tanshinone-loaded phase-transition ultrasonic contrast agent and a preparation method thereof. The method includes the preparation of glycyrrhetinic acid active ester, the preparation of lipid coating and the preparation of nanospheres by adopting an ultrasonic cell disruptor to perform ultrasonic processing. The grain size of the lipid nanospheres prepared by the invention is small, the average grain size is about 310nm, and passive targeting can be implemented by a tumor site EPR (Enhanced Permeability and Retention) effect. Moreover, glycyrrhetinic acid modification can also realize active targeting to the liver, consequently, the toxic and side effects of the preparation can be decreased, and the curative effect of the drug can be enhanced. On the other hand, the nanospheres which wrap low-boiling point liquid fluorocarbon with lipid as coating have good biocompatibility. Under certain conditions, enhanced ultrasonography can be performed by liquid-gas phase transition, the theranostics of tumors can be implemented after the anticancer drug tanshinone is loaded, consequently, intermediate links can be reduced, efficiency can be greatly increased, and the contrast agent has a broad clinical application prospect.

The invention discloses a rare earth upconversion diagnosis-and-treatment-integrated nanocomposite material. The rare earth upconversion diagnosis-and-treatment-integrated nanocomposite material is acuprous oxide modified rare earth upconversion nanocomposite prepared by the following steps: using oil-soluble upconversion nanoparticles (UCNPs) as cores; and then, allowing epitaxial growth of cuprous oxide (Cu(2)O) on surfaces of the UCNPs so as to form shells, thereby obtaining the cuprous oxide modified rare earth upconversion nanocomposite grown by interface nucleation. The Cu(2)O can transfer fluorescence resonance energy with upconversion emission of the UCNPs excited by near-infrared light so as to produce sufficient active oxygen, thereby meeting needs of photodynamic therapy; and moreover, the Cu(2)O can synchronously cooperate with the oil-soluble UCNPs so as to realize integrated diagnosis and treatment. The invention further provides a preparation method of the rare earth upconversion diagnosis-and-treatment-integrated nanocomposite material. The preparation method of the rare earth upconversion diagnosis-and-treatment-integrated nanocomposite material comprises the following steps: using the oil-soluble UCNPs as cores; and then, allowing interface nucleation growth of the (Cu(2)O) on the surfaces of the UCNPs. The rare earth upconversion diagnosis-and-treatment-integrated nanocomposite material prepared by the preparation method is uniform in size, good in stability, and excellent in biocompatibility; and thus, the nanocomposite material can be used in imaging diagnosis guided photodynamic therapy so as to meet needs of clinical diagnosis and treatment integration.

The invention provides an adriamycin-carrying lipid nanoscale ultrasound contrast agent targeting tumor-related fibroblasts and a preparation method of the adriamycin-carrying lipid nanoscale ultrasound contrast agent. The surface of the contrast agent is modified by a high affinity ligand (FH short peptide) for specifically expressing a TNC protein by the tumor-related fibroblasts, the FH short peptide is used as a targeting ligand, lipid is used as a shell membrane material, and adriamycin and gaseous fluorocarbon are wrapped inside the shell membrane. The targeted ultrasound contrast agentis in nanoscale, has ultrasound imaging capability, can be used for targeted delivery of drugs into the tumor-related fibroblasts, and specifically kills the tumor-related fibroblasts, and a tumor treatment effect is achieved through intervention on a tumor microenvironment.

The invention discloses a near infrared fluorescent dye taking a fluorophore of a cyanine dye as a parent skeleton structure and a preparation method and the application of the near infrared fluorescent dye, and belongs to the field of biomedical materials. The photostability of the near infrared fluorescent dye is better and the fluorescence quantum yield of the near infrared fluorescent dye is high as a cyclic hydrocarbon group is inserted into a long methenyl chain. The near infrared fluorescent dye can serve as a hydrophobic end to be combined with a hydrophilic molecule with good biocompatibility through an environment sensitive bond and self-assembled to form a nanoscale lipidosome or a micelle or a vesicle. An obtained lipid material can be mixed with a medicine and\or a gene and self-assembled to form the lipidsome or the micelle or the vesicle, and the medicine and\or the gene are\is coated inside to form a carrier system. The carrier system at the luminescence quenching state enters a target cell, the environment sensitive bond on the material is fractured and disassembled in the specific environment, the medicine and\or the gene are\is released to play a role in treatment, moreover, the cyanine dye gathered inside is also released, the fluorescence is recovered. Therefore, the fluorescence switching function is realized, and the integration of diagnosis and treatment is further realized.

The invention provides a tumor cell membrane drug loading system as well as a construction method and application thereof. The drug loading system comprises a tumor cell membrane and a polypeptide drug connected to the surface of the tumor cell membrane. The tumor cell membrane has good biocompatibility, so that the half-life period of the polypeptide drug in vivo can be prolonged; the tumor-associated antigen capable of being expressed on the surface of a tumor cell membrane can play a role of a tumor vaccine after being phagocytized by macrophages; homogeneous adhesion antigens can be expressed on the surfaces of tumor cells, so that the drug loading system is actively targeted to tumor parts, and active targeted drug delivery is realized. Meanwhile, the contrast agent is wrapped in the tumor cell membrane, and the contrast agent is gathered at the tumor part and the concentration of the contrast agent is increased by utilizing the active targeting property of the tumor cell membrane to the tumor part, so that the nuclear magnetic imaging effect of the tumor part is enhanced. Therefore, the tumor cell membrane drug loading system has important significance for realizing tumor diagnosis and treatment integration.

The invention relates to a novel diagnosis and treatment integrated hybridization micelle and a preparation method thereof. The hybridization micelle is a compound micelle which is self-assembled of two segmented copolymers through a dewatering effect. During preparation, the central nucleus of the compound micelle self-assembled of the two segmented copolymers through the dewatering effect is composed of an amphiphilic polymer hydrophobic chain segment and a hydrophobic medicine, a shell layer is a polymer hydrophilic chain segment, and special groups on the chain segment are used for generating magnetic nanoparticles on the shell layer in situ by a chemical coprecipitation method. Compared with the prior art, the preparation method has the advantages of being mild in preparation conditions and being simple, convenient and easy to carry out. The prepared micelle is stable in structure, has very good biocompatibility and biodegradability and can rapidly release medicine in a tumor environment, thus having the diagnosis-treatment integrated application potentiality.

The invention discloses a biodegradable unimolecular multi-support-arm polymer. The polymer comprises a hydrophobic core, a hydrophobic core shell layer and a hydrophilic outer shell layer, wherein the hydrophobic core is hyperbranched polyester, the hydrophobic core shell layer is polycaprolactone, and the hydrophilic outer shell layer is polyethylene glycol. The biodegradable unimolecular multi-support-arm polymer is applied to diagnostic integration nanometer medicine carrying polymer as a carrier, gadolinium and cisplatin can be wrapped into a molecular carrier hydrophobic section, and the water solubility of the cisplatin can be improved on the basis of the strong hydrophilcity of polyethylene glycol, so that the immunogenicity is reduced, and the circulation time of the gadolinium and cisplatin in the blood can be prolonged. Compared with the conventional magnetic resonance imaging (MRI) contrast medium and conventional chemotherapeutics, the diagnostic integration nanometer medicine carrying polymer can circulate in the blood for a long time, has small toxic or side effects and can wrap the gadolinium and the cisplatin at the same time, so that diagonistic integration is realized. The invention further discloses a preparation method and application of the biodegradable unimolecular multi-support arm polymer.

The invention provides peptide-drug conjugate of target placenta-like chondroitin sulfate A. The peptide-drug conjugate comprises a small molecule drug part, a peptide part and a connecting sub-part connected with the small molecule drug part and the peptide part, wherein peptide corresponding to the peptide part can target the placenta-like chondroitin sulfate A specifically, and the amino acid sequence of the peptide is selected from one or more of amino acid sequences shown as SEQ ID NO:1 to SEQ ID NO:3. The peptide-drug conjugate can target inappropriately-expressed target tissue of the placenta-like chondroitin sulfate A specifically, a drug is controlled to be released near the target tissue, and the toxic and side effects of the drug on normal tissue are reduced. The invention further provides a preparation method and application of the peptide-drug conjugate.

The invention discloses a magnetic targeting cell membrane modified ligand, a drug-loading material, a preparation method of the magnetic targeting cell membrane modified ligand and the drug-loading material and an application of the drug-loading material. The structural general formula of the magnetic targeting cell membrane modified ligand is shown as I series or II series in the formula (1). The magnetic targeting cell membrane drug-loading material is obtained by performing chemical covalent bond modification on a cell membrane to modify the magnetic targeting cell membrane modified ligand, an in-vitro test shows that the material is good in stability, can be effectively taken in by tumor cells, and has relatively high selectivity on the tumor cells, and besides, the material has remarkable paramagnetism. Under the condition of an external magnetic field, a magnetic targeting effect can be achieved. In an in-vitro anti-tumor test, the drug-loading material is remarkable in anti-tumor activity and hardly has toxicity to normal cells, so that the drug-loading material has a potential application of targeted therapy of malignant tumors.