46results about How to "Extend cycle time" patented technology

The invention relates to the technical field of atherosclerosis related drugs, in particular to a macrophage membrane functionalized manganese dioxide nano-drug as well as a preparation method and application thereof in treatment of atherosclerosis. The invention relates to a macrophage membrane functionalized manganese dioxide nano-drug, which comprises an HMnO2 carrier, colchicine is loaded on the HMnO2 carrier, and a macrophage membrane is coated outside the HMnO2 carrier. The preparation can be applied to practical operation of preparing medicines for treating atherosclerosis. According to the technical scheme, the technical problem of limited treatment effect caused by single action mechanism of medicines for treating atherosclerosis in the prior art can be solved. The nano-drug in the scheme can remove ROS to play an anti-inflammatory effect, reprogram macrophages and promote cholesterol excretion, and is used for synergistically treating atherosclerosis. The macrophage membrane can target an inflammation part and escape from an immune system. And the side effect of the nano-drug is relatively small, so that the nano-drug has ideal popularization and application prospects.

The invention relates to the technical field of medicine, and discloses an artificial exosome and application thereof in drug delivery, the artificial exosome is prepared into an oil phase based on PLGA and DOTAP, and a water phase based on OCT4 protein and siTrim28; mixing the two phases to obtain a primary emulsion, and adding a PVA aqueous solution to obtain a double emulsion; stirring the double emulsion and volatilizing the solvent to form an emulsified solution, and centrifuging to obtain PLGA nanoparticles; the lipid mixture is prepared from lipid, DPPE-PEG2000, alpha-tocopherol and alpha-lactalbumin, and a lipid film is formed after a solvent is evaporated; the preparation method comprises the following steps: mixing PLGA nanoparticles, a lipid film and membrane protein, uniformly mixing and heating to obtain a film-coated solution, and finally extruding through a cellulose acetate film to obtain the artificial exosome. The artificial exosome disclosed by the invention combines the stability of the PLGA nanoparticles and the biocompatibility of the lipid membrane, and provides new possibility for a drug delivery system.

The invention provides a PVDF-PMMA (Polyvinylidene Fluoride-Polymethyl Methacrylate)-based polymer electrolyte membrane as well as a preparation method and application thereof, and belongs to the field of lithium batteries. The invention provides a PVDF-PMMA (Polyvinylidene Fluoride-Polymethyl Methacrylate)-based polymer electrolyte membrane. The PVDF-PMMA-based polymer electrolyte membrane is prepared from the following components: PVDF, PMMA, a lithium salt, an active filler and a phosphine compound, the mass ratio of the PVDF to the lithium salt is (2-15): 1; the mass ratio of the PVDF to the PMMA is (1-10): 1; the ratio of the total mass of the PVDF and the lithium salt to the mass of the active filler is (2-30): 1; the ratio of the total mass of the PVDF and the lithium salt to the mass of the phosphine compound is (5-30): 1. The polarity of the phosphine compound is high, and the phosphine compound can promote dissociation of lithium salt, so that rapid and efficient transmission of lithium ions in the PVDF-PMMA-based polymer electrolyte membrane at room temperature is promoted, and the conductivity of the PVDF-PMMA-based polymer electrolyte membrane is further improved.

The invention discloses a trimethylolethane modified aqueous zinc ion battery electrolyte and an aqueous zinc ion battery. Wherein the aqueous zinc ion battery electrolyte comprises soluble zinc salt, trimethylolethane and water, after a trimethylolethane additive rich in polar groups (-OH) is introduced into the electrolyte, a zinc negative electrode can be covered by trimethylolethane molecules, and the adsorption of H2O is hindered, so that the side reaction is inhibited; and meanwhile, the adsorbed trimethylolethane adjusts the diffusion and nucleation behaviors of Zn < 2 + > on the surface of the negative electrode, and promotes the preferential deposition of a Zn (002) texture, so that dendrite-free Zn deposition is realized, and the problems of dendrite, hydrogen evolution, corrosion and the like of the current aqueous zinc ion battery negative electrode are solved. Besides, the trimethylolethane adsorbed on the surface of the electrode regulates the double electrode layer at the interface of the zinc negative electrode, so that the number of water molecules in the double electrode layer is reduced, the cycle time of the zinc negative electrode can be effectively prolonged, and the device life of the water-based zinc ion battery is prolonged.

The invention belongs to the technical field of medicines, and relates to a cholesterolized rapamycin liposome as well as preparation and application thereof. The invention relates to a cholesterylated rapamycin liposome, which is prepared from cholesterylated rapamycin, phospholipid and a phospholipid PEG (Polyethylene Glycol) derivative. The liposome is mainly composed of a rapamycin cholesterol conjugate, the coupling effect of cholesterol and rapamycin indirectly enables a drug and phospholipid to be tightly combined, compared with free rapamycin, the entrapment efficiency is greatly improved, the leakage rate is reduced (the entrapment efficiency is improved by 8.05%, and the leakage rate is reduced by 100%), the entrapment efficiency of the preparation is as high as 95% or above, and the preparation has good application prospects. The particle size is about 120nm, the PDI is less than 0.2, the drug can be stably delivered in vivo, the circulation time of the drug in vivo is prolonged, the toxic and side effects are reduced, the safety is improved, the stability is good, and the storage, transportation and use are facilitated.

The invention belongs to the technical field of energy storage, and particularly relates to a method for modifying a zinc negative electrode interface by using silver nanowires, which comprises the following steps: step 1, dispersing the silver nanowires with high length-diameter ratio into an organic solvent at a certain concentration; step 2, performing ultrasonic cleaning on the zinc foil, and then coating the silver nanowire dispersion liquid prepared in the step 1 on the surface of the zinc foil; 3, the zinc foil coated with the silver nanowire dispersion liquid in the step 2 is dried, and the zinc foil attached with the silver nanowires is obtained; and 4, the silver nanowires attached to the zinc foil in the step 3 are subjected to hot welding, and the zinc foil coated with the silver nanowire interconnection network is obtained. The silver nanowire conductive network formed on the surface of the zinc foil enables the current to be uniformly distributed, so that the formation and growth of zinc dendrites are inhibited, and the electroplating / stripping cycle time of the zinc negative electrode is remarkably prolonged; and the silver nanowire conductive network can also adjust the interface property of the zinc negative electrode and the electrolyte, reduce the corrosion current and enhance the stability of the electrode.

The invention belongs to the technical field of drug carriers, and particularly discloses a preparation method and application of a closed-loop feedback-regulation nano drug delivery system.The preparation method comprises the following steps that a starting material containing a reversible molecular library is provided, and the reversible molecular library is prepared in a buffer salt system; under thermodynamic control, adding anti-cancer drug molecules and guest molecules into the starting material; through a reversible covalent reaction process, molecules in an initial material spontaneously form a self-assembled nano-carrier containing drug molecules under the combined action of the drug molecules and guest molecules. According to the preparation method and application of the closed-loop feedback-regulation nano drug delivery system, reversible covalent reaction, closed-loop feedback-regulation and self-assembly technologies are combined, spontaneous construction of a nano carrier is achieved, and the nano carrier is endowed with excellent drug loading capacity, stability and environmental responsiveness.

The utility model provides novel corrosion-resistant high-efficiency packing, which relates to the technical field of chemical packing and comprises a packing main body and a packing hoop, the packing hoop is arranged on the outer surface of the packing main body, an L-shaped splicing groove is formed in the side surface of the packing hoop, an installation screw hole is formed in the center of the surface of the packing main body, and a guide screen plate is arranged on the bottom surface of the installation screw hole. The two sets of filler bodies are arranged, the splicing assemblies are arranged between the adjacent filler bodies, the two ends of the splicing assemblies are connected with the L-shaped splicing grooves, the mounting studs are inserted into the mounting screw holes, and the adjacent filler bodies are spliced through the splicing assemblies, so that the two filler bodies form one set to be mounted and used, and a sandwich structure is formed for a gas-liquid path; the circulating time of gas and liquid in the tower is prolonged, the gas-liquid separation and flow guide efficiency is improved, meanwhile, the splicing assemblies composed of the filler plates are arranged for use, so that when the surface is damaged, rapid replacement can be achieved, the structure is simple, gas and liquid are treated through multiple channels, corrosion is prevented, and use in the chemical tower is facilitated.

The invention discloses an oligomeric amino acid dendritic macromolecular material as well as a preparation method and application thereof, and relates to the technical field of nano materials. The general formula of the oligomeric amino acid dendritic macromolecular material is TPE-(PEG) n-(Lys) x-(Arg) y, wherein TPE is a hydrophobic core, has an aggregation-induced emission characteristic, and can enhance the self-assembly capability and the tracing function of the nano-carrier; pEG is a connecting arm, so that the water solubility and biocompatibility of the material can be improved; lys is a branch skeleton and is a main construction unit of a dendritic macromolecule; arg is a functional terminal, guanidyl of Arg has strong positive charges, electrostatic binding with negatively charged nucleic acid can be promoted, and meanwhile cellular uptake can be enhanced possibly through the membrane penetration effect. The oligomeric amino acid dendritic macromolecular material gives consideration to stability, modifiability and targeting, provides a new scheme for small nucleic acid delivery, and is expected to have far-reaching influence in the fields of precise tumor treatment, gene therapy and multi-modal diagnosis and treatment.

The utility model belongs to the technical field of nitrogen-containing sewage treatment, and particularly relates to a short-range denitrification reactor which comprises a reaction tank, a cooling and stirring assembly is mounted in the reaction tank and comprises a coiled pipe, the two ends of the coiled pipe penetrate through the reaction tank and are rotationally connected with the reaction tank, and the cooling and stirring assembly is mounted in the reaction tank. The two ends of the coiled pipe are fixedly connected with rotating joints respectively; according to the sewage treatment device, the first driver drives the coiled pipe to rotate, so that stirring liquid is stirred, fusion of a medicament and sewage is accelerated, reaction is promoted, cold water in the water storage tank is guided into the coiled pipe by the circulating pump to form circulating flow, and then heat exchange with the sewage is performed when the coiled pipe rotates; when the coiled pipe rotates, the coiled pipe can be in full contact with the sewage in the reaction tank, and compared with the prior art, the cooling effect of the sewage in the reaction tank is integrally improved.

The invention discloses ultra-coarse tungsten carbide and a preparation method thereof, and the preparation method comprises the following steps: providing ultra-coarse tungsten oxide particles with the water content of 3-5 wt%, the average particle size of the ultra-coarse tungsten oxide particles being 28-45 [mu] m; loading the ultra-coarse tungsten oxide particles into a boat, placing the boat in a closed reduction furnace, introducing hydrogen, and carrying out a reduction reaction to obtain ultra-coarse tungsten particles; wherein the temperature of the reduction reaction ranges from 950 DEG C to 1100 DEG C, the boat loading amount ranges from 2.8 Kg / boat to 3.5 Kg / boat, the circulation time ranges from 45 min / boat to 60 min / boat, the hydrogen flow ranges from 8 m < 3 > / h to 15 m < 3 > / h, and the hydrogen dew point ranges from-65 DEG C to-75 DEG C; the ultra-coarse tungsten particles and carbon are mixed, and a carbon mixed material is obtained; performing high-temperature carbonization on the carbon-containing mixture to obtain tungsten carbide particles; the tungsten carbide particles are crushed and sieved, the ultra-coarse tungsten carbide is obtained, and the average particle size of the ultra-coarse tungsten carbide ranges from 30 micrometers to 60 micrometers. According to the method, the ultra-coarse tungsten particles with high purity and more uniform particle size distribution can be obtained.

This invention discloses nanoparticles that enhance the near-infrared II fluorescence of indocyanine green, their preparation method, and applications. The nanoparticles are prepared by mixing PLGA-PEG-PLGA and ICG-NHS. The specific method is as follows: S1, dissolve PLGA-PEG-PLGA in a solvent to form a first solution; S2, dissolve ICG-NHS in a solvent to form a second solution; S3, thoroughly mix the first and second solutions, react them, and remove unreacted substances by ultrafiltration to obtain the nanoparticles. The order of steps S1 and S2 is not limited. This invention enhances the emission of ICG through PLGA-PEG-PLGA, extending the application of ICG in the near-infrared II region to the NIR-IIa region (1300nm-1400nm), and effectively prolongs the circulation time of ICG in the blood, thus removing limitations in its application in fields such as tumor and vascular disease diagnosis.

The invention provides a tumor targeted nano drug delivery system and a preparation method and application thereof, the tumor targeted nano drug delivery system comprises a PD-L1 monoclonal antibody, radix pseudostellariae cyclic peptide B and an mPEG-PLGA-NH2 nano carrier, the radix pseudostellariae cyclic peptide B is encapsulated in the mPEG-PLGA-NH2 nano carrier, and the outer surface of the mPEG-PLGA-NH2 nano carrier is modified with the PD-L1 monoclonal antibody. The tumor-targeted nano drug delivery system has good anti-cancer activity and biocompatibility, has longer circulation time and higher bioavailability, is obviously superior to free drugs in the aspects of tumor resistance and immunoregulation, and is expected to be applied to preparation of tumor-targeted drugs, especially gastric cancer-targeted drugs.

The invention relates to a self-assembled modified polyolefin diaphragm as well as a preparation method and application thereof, and belongs to the technical field of electrochemistry. The technical problems that in the prior art, a polyolefin diaphragm is poor in thermal stability, mechanical performance and wettability, the side reaction of an electrode-diaphragm interface is serious, and dendritic lithium dendrites easily cause internal short circuit of a battery are solved. The preparation method of the self-assembled modified polyolefin diaphragm comprises the following steps: performing deposition modification of layered hydroxide on the surface of the polyolefin diaphragm through vacuum filtration to obtain a modified layer, and performing phase interface conversion on the modified layer through an electrochemical in-situ method to form a stable transition interface layer. The preparation method has the advantages of low production cost, simple operation process and the like, and the diaphragm prepared by the method has good thermal stability, mechanical property and chemical activity, can inhibit dendritic crystal growth to realize long-life cycle of the battery, and is suitable for large-scale production and industrial application.

The application discloses a pPAM long-circulating liposome, a preparation method and application thereof, and the long-circulating liposome takes a blank liposome as a carrier, internally carries a plasmid pPAM, and is decorated on the surface with a transferrin-penetratin complex Tf-Pen and an oxytocin receptor antibody OTR Ab； The plasmid pPAM carries a cDNA sequence of a peptide acylglycine alpha amidation monooxygenase PAM; and the blank liposome is composed of DSPC, PEG 2000 , cholesterol and lecithin. The application provides a new direction for development of endogenous polypeptide synthesis enzyme delivery technology and mental drugs, and improves the possibility of preparing new anti-anxiety and depression drugs, relieving mental stress and improving patient compliance.

The invention belongs to the field of new auxiliary materials and new dosage forms of pharmaceutical preparations, and particularly relates to construction of albumin-bound adriamycin prodrugs and prodrug self-assembled nanoparticles and application of the albumin-bound adriamycin prodrugs and the prodrug self-assembled nanoparticles in preparation of antitumor drugs. The invention provides a novel albumin binding type adriamycin prodrug, self-assembled nanoparticles containing the prodrug are constructed, and the in-vivo circulation time of the drug is remarkably prolonged through specific binding of maleimide or acryloyl chloride and in-vivo albumin, so that tumor targeting of the drug is realized; meanwhile, the drug can be intelligently released in tumor cells due to the reduction-sensitive disulfide bond, the toxic and side effects on normal cells are reduced, and the drug has a wide clinical transformation prospect.

This invention discloses a liposome, a drug-loaded liposome, its preparation method, and its applications. The liposome comprises a liposome backbone and a targeting ligand; wherein the liposome backbone comprises the following components: phospholipids, cholesterol, cationic lipids, and PEGylated phospholipids; the cationic lipids are (2,3-dioleoyl-propyl)-trimethylammonium chloride and / or 1,2-dioleoyl-3-trimethylammonium propane; and the targeting ligands are sodium hyaluronate and / or hyaluronic acid. This liposome possesses excellent basic properties and excellent targeting ability; after encapsulating the active pharmaceutical ingredients (doxacin hydrochloride and berberine hydrochloride), its targeting ability can be further enhanced in practical applications, thereby increasing its efficacy.

The invention discloses a hybrid cell membrane disguised hexagonal boron nitride nano-particle as well as a preparation method and application thereof, relates to the technical field of medicines, and aims to solve the problems of poor targeting property and biocompatibility of a boron carrying agent, insufficient in-vivo stability and tumor accumulation capacity of nano-particles and the like in BNCT tumor treatment in the prior art. The composite material comprises hexagonal boron nitride nanoparticles and a hybrid cell membrane coating the peripheries of the hexagonal boron nitride nanoparticles, and the hybrid cell membrane is formed by fusing a macrophage cell membrane and a tumor cell membrane. The targeting property and biocompatibility of the boron carrying agent and the in-vivo stability and tumor accumulation capacity of nanoparticles can be effectively enhanced, and the BNCT treatment effect of glioma is improved.

The invention belongs to the technical field of biological materials, and relates to a blood brain barrier crossing drug delivery system and a preparation method thereof, and the drug delivery system is a liposome prepared from 1-stearoyl-sn-glycerol-3-phosphorylcholine (LPC). The LPC is selected as an important component of the drug delivery system with the blood brain barrier crossing capacity for the first time, and the drug delivery system has the advantages of being good in stability, high in brain entering efficiency, long in circulation time and the like. Different from the traditional non-specific passive transportation and invasive brain entering modes, the method has the capability of efficiently delivering target molecules such as drugs to a brain area in a non-invasive targeting manner, and has extremely strong clinical transformation significance; and the synthesis route is simple, the reaction condition is mild, and the damage to the possibly carried target compound is small.

The utility model discloses a methanol vaporizer with inner fins. The methanol vaporizer comprises a vaporizer body, a liquid inlet pipe is arranged close to the upper end of one side of the device body, and a liquid outlet pipe is arranged close to the lower end; the heat exchanger further comprises an inner sleeve, fins and turbulent flow blocks. The fins are fixed to the outer side of the inner sleeve at intervals, and the outer side faces of the fins are fixedly connected with the inner side face of the device body. Notches are formed in the fins, and the positions of the notches in the adjacent fins are different; the end of the liquid inlet pipe extends to the position above the fin at the uppermost end, and the end of the liquid outlet pipe extends to the position below the fin at the lowermost end. The heat exchanger is good in heat exchange effect, and the influence on a heating assembly in the heat exchanger body is small.

The invention provides a quaternary high-entropy alloy nano enzyme as well as a preparation method and application thereof, and belongs to the technical field of nano biological materials and biological medicines. The quaternary high-entropy alloy nano-enzyme comprises a single-phase alloy and polyvinylpyrrolidone, the single-phase alloy is composed of four metal elements of M, rhodium, iridium and ruthenium, the surface of the single-phase alloy is modified with the polyvinylpyrrolidone, the element M is selected from palladium or platinum, and the particle size of the quaternary high-entropy alloy nano-enzyme is smaller than 10 nm. The quaternary high-entropy alloy nano-enzyme disclosed by the invention can penetrate through a blood brain barrier, is enriched at a cerebral hemorrhage focus, and comprehensively blocks reactive oxygen species (ROS) cascade reaction after cerebral hemorrhage.

The invention provides a nano preparation for preparing a medicine for inhibiting prostatic cancer and a preparation method of the nano preparation, and relates to the technical field of nano medicinal preparations. The nano preparation comprises the following components in parts by mass: traditional Chinese medicine components and a nano carrier, the traditional Chinese medicine component comprises 15-45 parts of a Chinese yam extract, 5-15 parts of a madder extract, 8-20 parts of cuttlebone superfine powder, 10-25 parts of dragon bone superfine powder and 10-25 parts of oyster superfine powder; the nano-carrier comprises 5-20 parts of phospholipid, 2-10 parts of cholesterol, 1-5 parts of fatty acid, 3-15 parts of polyethylene glycol and 1-5 parts of a surfactant. The average particle size of the nano preparation is 80-150 nm, the polydispersity index PDI is smaller than or equal to 0.30, and the encapsulation efficiency is larger than or equal to 75%. According to the invention, active ingredients of traditional Chinese medicines are combined with a nanotechnology, so that the bioavailability and the tumor targeting property are remarkably improved, the pH-responsive release characteristic is achieved, a remarkable inhibition effect on various prostate cancer cell lines is shown, tumor growth can be effectively inhibited, metastasis can be reduced, and a good application prospect is achieved.

The invention belongs to the technical field of medicinal chemistry and medicinal preparations, and particularly relates to an anti-tumor compound, a self-assembled nano drug-loading system of the anti-tumor compound and a preparation method and application of the self-assembled nano drug-loading system. According to the novel antitumor compounds CPT-s-s-Lindeqvist and CPT-c-c-Lindeqvist and the preparation method thereof, the synthesis method of the two compounds is simple and convenient, and the raw material cost is low. CPT-s-Lindeqvist or CPT-c-c-Lindeqvist is prepared into vesicles through a film hydration method to obtain the self-assembled nano drug delivery system, accurate drug release in tumor tissue can be achieved, a certain slow release effect is shown, and the self-assembled nano drug delivery system has good biological safety and remarkable synergistic anti-tumor effect.

The invention discloses a BODIPY dye with an annular structure as well as a preparation method and application thereof, relates to the technical field of medical dyes, and solves the problems of stability of the BODIPY dye in an aqueous medium and safety in a living body. The preparation method comprises the following steps: dissolving a compound A and dicarboxylic acid in anhydrous dichloromethane, adding 1-ethyl-(3-dimethylaminopropyl) carbodiimide hydrochloride and a catalyst, carrying out a reaction under the protection of nitrogen, and carrying out silica gel column chromatography purification on a crude product to obtain the BODIPY dye with the cyclic structure. The amphipathy of the cyclic BODIPY formed by ester bonds or ether bonds is increased, so that the cyclic BODIPY forms nanoparticles in an aqueous solution, the BODIPY photosensitizer is delivered in a living body, the stability of the BODIPY photosensitizer in water is enhanced, the in-vivo circulation time is prolonged, the safety of the BODIPY photosensitizer in the living body is improved, and the integration of tumor diagnosis and treatment in the living body can be realized.

This invention belongs to the field of medicinal chemistry, specifically relating to a mitochondrial-dequinoline chloride-hyaluronic acid compound, its preparation method, and its application. The rotenol-dequinoline chloride-hyaluronic acid compound has the following structural formula, where n is 2. Based on the anticancer activity of rotenone, this invention utilizes reduction, esterification, and two amide reactions to successfully link rotenol, dequinoline chloride, and hyaluronic acid via amide bonds. Subsequently, these components are successfully self-assembled into spherical nanoparticles using ultrasound, constructing a rotenol-dequinoline chloride-hyaluronic acid nanosystem for tumor therapy. After targeted delivery of these nanoparticles to tumor cells, both rotenol and dequinoline chloride synergistically act on the mitochondria, forming a dual-drug delivery system that generates a large amount of ROS, thereby inducing apoptosis and effectively inhibiting tumor growth and migration.

This invention relates to the field of secondary battery technology, and discloses an inorganic superionic conductor material, its preparation method, and its applications. The inorganic superionic conductor material comprises apatite and dopant ions doped into the apatite; wherein the dopant ions include metal cations and non-metal anions. The inorganic superionic conductor material provided by this invention can be used as a modified filler to achieve synergistic effects on electrolyte mechanical and electrochemical performance. Simultaneously, it can be introduced as a modifier into iodine cathodes to activate the 4-electron reaction of the iodine cathode, and introduced into bromine cathodes to stabilize the redox reaction of bromine, reducing bromine loss, thereby significantly improving the capacity and cycle stability of the full battery. Furthermore, by using 3D printing technology, screen printing, digital light processing, and fused deposition modeling, the inorganic superionic conductor material can be assembled with interdigitated positive and negative electrodes into flexible batteries, exhibiting excellent application effects when integrated with flexible LEDs.

The invention relates to a plasmid-loaded targeted liposome compound, and a preparation method and application thereof. The liposome compound comprises liposome and plasmid pPEA entrapped by the liposome, and the plasmid pPEA comprises an expression vector pGAVPO containing a blue light induced transcription factor GAVPO protein and pU5-PEA of a light-operated gene expression system capable of expressing PEA. The targeting biological material for modifying the liposome is fused on the liposome to form a liposome compound pPEA (at) GPH-L entrapping the pseudomonas aeruginosa exotoxin light-operated gene and the liposome targeting material. The liposome disclosed by the invention can target tumor cells, has great lethality on the tumor cells, is safe and effective, and has important significance in preparation of anti-tumor medicines, especially medicines for treating breast cancer.

The invention discloses a preparation method of IR780 and gambogic acid prodrug-loaded lipidosome, which comprises the following steps: dissolving mPEG-TK-NH2 in dimethylformamide, adding gambogic acid, benzoyl peroxide and triethylamine, stirring for reaction, concentrating and recrystallizing to obtain mPEG-TK-GA, adding mPEG-TK-GA, soybean phosphatidylcholine, cholesterol and IR780 into an organic solvent, and carrying out rotary evaporation to remove the organic solvent, thereby obtaining the IR780 and gambogic acid prodrug-loaded lipidosome. The SIRP-alpha gene engineering exosome preparation method comprises the following steps: preparing a lipid membrane, adding a phosphate buffer solution, heating, carrying out ultrasonic treatment, granulating to obtain a liposome solution, mixing an SIRP-alpha gene engineering exosome and IG Lipo, and carrying out membrane fusion by adopting a freeze-thaw method to obtain the SIRP-alpha gene engineering exosome preparation. Compared with the prior art, the liposome prepared by the method has a responsive drug release effect, can relieve toxic and side effects while improving the treatment effect, is also loaded with IR780, and can reduce the dosage and improve the treatment effect in combination with photothermal therapy.

This invention provides the application of the LHPP gene in liver cancer immunotherapy and liver cancer immunotherapy drugs. Reagents that promote LHPP gene expression can be used to prepare liver cancer immunotherapy drugs. This invention also provides an mRNA nanomedicine, which is prepared by the following method: [The invention then describes a process involving Meo-PEG-Dlink]. m A PLGA solution, an aqueous solution of LHPP gene mRNA, and a cationic liposome solution were mixed and added dropwise to water under stirring to obtain a nanosolution. The nanosolution was then transferred to an ultrafiltration membrane and centrifuged. This invention is the first to discover that LHPP can participate in the immunotherapy of liver cancer and is associated with the survival prognosis of liver cancer. LHPP enhances STAT1 nuclear translocation by affecting the expression of autophagy-regulated IFNβ, thereby promoting the expression of T cell-related chemokines secreted by liver cancer cells, and ultimately promoting CD8+ expression. + T cell infiltration. Therefore, the LHPP gene can serve as a novel target for immunotherapy of liver cancer. The mRNA nanomedicine described has advantages such as long circulation time in the blood, high accumulation in tumors, rapid mRNA release, and low cost.

The invention relates to a preparation method of a metal ion complexation mRNA delivery system and a product and application thereof, and the preparation method comprises the following steps: (1) mixing mRNA and metal ions in a solvent at 10-35 DEG C for 10-60 min to obtain nanoparticles; and (2) mixing the nanoparticles with a phospholipid membrane at 10-35 DEG C for 2-60 minutes, and wrapping the nanoparticles with the phospholipid membrane to obtain the metal ion complexation mRNA delivery system. Metal ions and mRNA are mixed and complexed to form complex nanoparticles, and mRNA loading capacity can be improved. The surfaces of the nanoparticles are coated with phospholipid membranes, so that the biocompatibility of the delivery system is improved, the in-vivo circulation time of the delivery system is prolonged, and meanwhile, fusion between the delivery system and a target cell membrane can be promoted. Besides, the mixing temperature of mRNA and metal ions is controlled to be 10-35 DEG C, the mixing time is controlled to be 10-60 min, the reaction condition is mild and rapid, the prepared nanoparticles are uniform in particle size, and safer and more effective mRNA delivery can be achieved.