The invention discloses a bionic nano-carrier and an application thereof in preparation of brain glioma treatment medicines, and belongs to the technical field of medicines. The bionic nano-carrier comprises a long-circulating liposome and a hybrid cell membrane; the long-circulating liposome serves as an inner core, the hybrid cell membrane serves as a shell, and the long-circulating liposome is coated with the hybrid cell membrane; and the hybrid cell membrane is formed by mixing an immune cell membrane and a tumor cell membrane. According to the nano-carrier disclosed by the invention, the long-circulating liposome is taken as a core, the core is coated with a hybrid cell membrane of immune cells and tumor cells, the nano-carrier moves and passes through a blood-brain barrier through the interaction of surface protein of the immune cells and receptors on brain endothelial cells, and meanwhile, the enhanced tumor targeting capability is realized through homologous targeting and homing effects, targeted treatment of brain glioma is achieved, and the chemotherapy effect is improved.