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320 results about "Gene carrier" patented technology
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Carrier (Genetics) In genetics, the term carrier describes an organism that carries two different forms (alleles) of a recessive gene (alleles of a gene linked to a recessive trait) and is thus heterozygous for that the recessive gene.
Drugs And Gene Carrier Particles That Rapidly Move Through Mucous Barriers
ActiveUS20080166414A1Easy adhesionPromote complexationPowder deliveryOrganic active ingredientsCrystallographyMucus
Owner:THE JOHNS HOPKINS UNIVERSITY SCHOOL OF MEDICINE
Biodegradable mixed polymeric micelles for gene delivery
InactiveUS6210717B1Kind be easilySimple contentOrganic active ingredientsNanotechGene deliveryPolyester
A biodegradable, mixed polymeric micelle used to deliver a selected nucleic acid into a targeted host cell contains an amphiphilic polyester-polycation copolymer and an amphiphilic polyester-sugar copolymer. The polyester-polycation copolymer forms an electrostatic interaction with polyanionic nucleic acids, and the polyester-sugar copolymer directs the micelle-nucleic acid complex to cells in vivo. Additional copolymers with similar properties may also be included. The composition improves delivery efficiency by providing a particulate gene carrier for which particle size and charge density are easily controlled by multivariate means. Various kinds of ligands and other functional compounds may be also be introduced using the composition. The composition may be used in a method for transforming a targeted host cell with a selected nucleic acid.
Owner:SAMYANG BIOPHARMLS CORP
Drugs and gene carrier particles that rapidly move through mucous barriers
ActiveUS8957034B2Easy adhesionPromote complexationPowder deliveryLiquid surface applicatorsActive agentMedicine
Owner:THE JOHNS HOPKINS UNIVERSITY SCHOOL OF MEDICINE
Water soluble chitosan nanoparticle for delivering an anticancer agent and preparing method thereof
The present invention relates to a water soluble chitosan nanoparticle (WSC-NP) for delivering an anticancer agent and a preparing method thereof, more precisely, a water soluble chitosan nanoparticle for delivering an anticancer agent which has function of targeting on a wanted area by introducing a functional group in the location of highly reactive amine group and becomes an excellent gene carrier with the use of water soluble chitosan since the water soluble chitosan itself can combined with DNA having a negative electric charge(−) owing to the very strong positive electric charge(+) of its amine group, and a preparing method thereof. Therefore, a water-soluble chitosan nanoparticle for delivering an anticancer agent of the present invention can effectively envelope paclitaxel by introducing hydrophilic and hydrophobic groups in the position of highly reactive amine group of the water-soluble chitosan. A water soluble chitosan nanopaclitaxel prepared as the above has an excellent re-dispersion force, after freeze-drying, in distilled water and has an outstanding anticancer effect with its accumulation in tumor cells greater than that of the other anticancer agent carriers.
Owner:JEONG TUK RAI +1
Mucus Penetrating Gene Carriers
ActiveUS20130323313A1Prevent complete digestionPowder deliveryPeptide/protein ingredientsNanoparticleSide chain
Nanoparticles gene carriers, particularly nanoparticle gene carriers which exhibit increased rates of diffusion through cystic fibrosis (CF) mucus, as well as methods of making and using thereof, are described herein. The nanoparticle gene carriers are formed from a nucleic acid complexed to one or more biocompatible, polycationic polymers. The nanoparticle gene carriers also contain one or more mucus resistant polymers. In a particularly preferred embodiment, the nanoparticle gene carrier is a nanoparticle formed from one or more nucleic acids, PEI, and a mucus-resistant / diffusive graft copolymer composed of a PEI backbone functionalized by one or more PEG side chains. The nanoparticle gene carriers can efficiently diffuse through CF mucus, and can effectively serve as a vehicle to administer one or more nucleic acids to a patient suffering from CF.
Owner:THE JOHN HOPKINS UNIV SCHOOL OF MEDICINE
Hyperbranched polyamino acid, preparation method and application thereof
InactiveCN101575412AImprove responseEfficient transductionOther foreign material introduction processesChemical compositionArginine
The invention relates to hyperbranched polyamino acid. The chemical composition is as follows: in the formula of (Lys-)a-(-Arg-)b-(-His-)c-(-X)d, Lys, Arg and His are respectively lysine, arginine and histidine, X is one of or a plurality of any amino acids except for the amino acids above, wherein a is not less than 0.2 and not more than 0.95, b is not less than 0 and not more than 0.8, c is not less than 0 and not more than 0.8, d is not less than 0 and not more than 0.3, and the sum of a, b, c and d is 1. The preparation method is as follows: uniformly mixing lysine or lysine salt with other amino acids or the amino acid salts contained in the hyperbranched polyamino acid to react for 2-64h at the temperature of 130-180 DEG C and then obtain yellow solid, and finally purifying the coarse product with a precipitation method to obtain the hyperbranched polyamino acid. The hyperbranched polyamino acid obtained in the invention can be used as nonviral gene carrier for the transduction of huamn or animal cell gene, and the hyperbranched polyamino acid nonviral gene carrier provided by the invention is reasonable in design, simple in preparation method, high in transfection efficiency, low cytotoxicity and favorable transduction efficiency in the presence of blood serum.
Owner:WUHAN UNIV
Mucus penetrating gene carriers
ActiveUS9327037B2Prevent complete digestionPowder deliveryPeptide/protein ingredientsSide chainNanoparticle
Nanoparticles gene carriers, particularly nanoparticle gene carriers which exhibit increased rates of diffusion through cystic fibrosis (CF) mucus, as well as methods of making and using thereof, are described herein. The nanoparticle gene carriers are formed from a nucleic acid complexed to one or more biocompatible, polycationic polymers. The nanoparticle gene carriers also contain one or more mucus resistant polymers. In a particularly preferred embodiment, the nanoparticle gene carrier is a nanoparticle formed from one or more nucleic acids, PEI, and a mucus-resistant / diffusive graft copolymer composed of a PEI backbone functionalized by one or more PEG side chains. The nanoparticle gene carriers can efficiently diffuse through CF mucus, and can effectively serve as a vehicle to administer one or more nucleic acids to a patient suffering from CF.
Owner:THE JOHN HOPKINS UNIV SCHOOL OF MEDICINE
Recombination interferon with new space conformation and enhanced effect, its preparing method and application
ActiveCN1740197AStrong antiviral activityLittle side effectsPeptide/protein ingredientsAerosol deliveryAntigenSide effect
The invention provides recombination interferon (rSIFN-co) or the functional analog with new space conformation, enhanced effect, and low side effect. It is showed by the external pharmacodynamics, it can not only restrain the DNA replication of the hepatitis b virus, but also restrain the exudation of the surface antigen (HBsAg) and e antigen (HBeAg). The cytology toxicity is 1 / 8 of the clinical interferon and the antiviral activity is 5-20 times to the clinical interferon and has a higher, a more board spectrum and longer time biological response reaction, and prevent the tumor hyperplasia and transmission. The invention also provides the synthetic gene code, gene carrier, expression system of the synthetic gene of the recombination interferon or the functional analog. Finally, the invention also provides the preparing method and application thereof.
Owner:SUPERLAB FAR EAST LTD
Polyethylene glycol monomethyl ether-poly 2-methyl-carboxyl propylene carbonate graft polyethyleneimine copolymer, preparation method thereof and application thereof
The invention relates to polyethylene glycol monomethyl ether-poly 2-methyl-carboxyl propylene carbonate graft polyethyleneimine copolymer, a preparation method thereof and application thereof. The preparation method comprises the step of directly condensing carboxyl in the polyethylene glycol monomethyl ether-poly 2-methyl-carboxyl propylene carbonate graft polyethyleneimine segmented copolymer with amino in polyethyleneimine to form the graft copolymer. The copolymer is a polycation gene carrier, integrates the advantages of polyethylene glycol, Makrolan and polyethyleneimine, and has high transfection efficiency, wherein the highest transfection efficiency to the medium luciferase plasmid of african green monkey kidney cell is 14 times of that of American Invitrogen biological company transfection reagent Lipofetamine<TM>2000, can effectively antagonize the inhibiting effect of blood serum to the transfection, and has less cell toxicity, wherein consistency thereof is not higher than 200 microgramme / ml and cell survival rate is more than 80%.
Owner:CHANGCHUN INST OF APPLIED CHEMISTRY - CHINESE ACAD OF SCI
Cationic alpha-amino acid-containing biodegradable polymer gene transfer compositions
The invention provides gene transfer compositions using as the gene carrier a biodegradable polymer that contains one or more cationic alpha amino acids, such as arginine or agmatine. The compositions form a tight soluble complex with a poly nucleic acid suitable for transfecting target cells to effect translation of the cargo poly nucleic acid by the target cell. Thus, such compounds are useful both in vitro and in vivo.
Owner:MEDIVAS LLC
Biocompatible and biodegradable elastomer
ActiveUS20140194533A1Significant blood coagulation functionGood biocompatibilityPharmaceutical delivery mechanismPharmaceutical non-active ingredientsPolymer scienceBiocompatibility
The present invention provides a biocompatible and biodegradable elastomer, comprising a hard segment and a soft segment. The hard segment is formed by reacting diisocyanate and a chain extender; and the soft segment is comprising a biodegradable oligomer diol, wherein the biodegradable oligomer diol is selected from the group consisting of polycaprolactone diol, polyethylene butylene adipate diol (PEBA diol), poly-L-lactic acid diol (PLLA diol), polylactic acid diol and any combination thereof. The biocompatible and biodegradable elastomer of present invention can be used to produce vascular graft, cell carrier, drug carrier or gene carrier.
Owner:NAT TAIWAN UNIV
Method for whole surrounding surface display of target proteins using bacterial exosporium
InactiveUS8030064B2Fast conductionAddressing slow performanceBacteriaPeptide/protein ingredientsSurface displayScreening method
Owner:KOREA ADVANCED INST OF SCI & TECH +1
Nts-polyplex nanoparticles system for gene therapy of cancer
InactiveUS20150307554A1Specificity of thinAvoid developmentPowder deliveryPeptide/protein ingredientsCancer cellWhole body
The present invention describes a system of gene carrier nanoparticles capable of specifically internalize into cancer cells, eg, cancer cells involved in breast cancer, in vitro and in vivo. The system described allows the introduction of therapeutic genes specifically into target cells through NSTR1 receptor-mediated endocytosis of said system, making it possible to provide treatment for this type of conditions, for example by systemic, intravenous, or in situ administration.
Owner:CASTILLO RODRIGUEZ ROSA ANGELICA
Tetrahymena transgenic carrier containing HSP70 promoter and GFP and preparation method and use thereof
InactiveCN101586119AImprove relevanceIncrease credibilityMicrobiological testing/measurementChemiluminescene/bioluminescenceGene vectorBiology
The invention discloses a tetrahymena transgenic carrier containing HSP70 promoter and GFP and preparation method and use thereof. Plasmid T-GFP is constructed by using plasmid rDNA-GFP in the methods such as enzyme cutting and PCR enzyme cutting site mutation; thermophile tetrahymena genome DNA is used for constructing plasmid T-HSP702 in the methods such as PCR augmentation and clone; Plasmids T-GFP, T-HSP702 and pD5H8 are used for construction and finally obtaining plasmid HSP702-GFP-pD5H8 by enzyme cutting and connection. The carrier contains HSP702 promoter, thereby being capable of realizing high-efficient expression of exogenous gene; the carrier contains replaceable exogenous gene gfp so that an exogenous gene expression system is realized for tetrahymena by replacing different exogenous genes; with the sieving effect of paromomycin medicine, the carrier replaces most rDNAs originally in the tetrahymena so that the exogenous genes are largely increased in the tetrahymena so as to realize genetic transformation of target genes. The method in the invention is easy and has convenient operation, and the carrier can be used for detection of environmental tributyl tin in transfection in tetrahymena.
Owner:INST OF AQUATIC LIFE ACAD SINICA
Method of detection of transcription factor expression activity by luciferase reporter gene system
InactiveCN106399461ALow costGood price advantageMicrobiological testing/measurementBiological material analysisMarket potentialTime-Consuming
The invention provides a method of detection of transcription factor expression activity by a luciferase reporter gene system; the method comprises the steps: based on the luciferase characteristics of being lasting, stable and easy to detect, co-transfecting cells with a luciferase reporter gene vector containing a target gene promoter and a transcription factor expression plasmid; after the cells are cultured, carrying out lysis of a certain number of cells with a cell lysis buffer liquid, collecting a lysate containing luciferase, and centrifuging to take a supernatant; and then real-timely detecting luciferase expression intensity data in a luciferase activity detection buffer liquid, at the same time, detecting the luciferase concentration, correcting, and then calculating to obtain the transcription factor expression quantity, namely the transcription factor expression activity needing to detection. The method is simpler and more feasible in whole operation process, shorter in time consuming, higher in flux, relatively high in repeatability and accuracy, and lower in operating costs, thereby having broad application prospects, and having excellent market potential.
Owner:MIAOSHUN SHANGHAI BIOTECH CO LTD
Target quaternary ammonium salt cationic polymer lipid gene carrier, preparation method and application thereof
InactiveCN102234658ARich varietyHigh transfection efficiencyGenetic material ingredientsMacromolecular non-active ingredientsPositive controlQuaternary ammonium cation
The invention discloses a target quaternary ammonium salt cationic polymer lipid gene carrier, a preparation method and an application thereof. The target quaternary ammonium salt cationic polymer lipid genetic carrier is characterized in that: a polymeric quaternary ammonium salt and lipid are adopted for preparing a quaternary ammonium salt cationic polymer lipid genetic carrier according to a mass ratio, wherein the mass ratio of the polymeric quaternary ammonium salt to the lipid is 0.05-20:1; then a assembly method or a modification method is adopted for modifying to prepare a folic acid or EGFR antibody modified cationic polymer lipid gene carrier. Results of gene transfection experiments show that: gene transfection efficiencies of the target quaternary ammonium salt cationic polymer lipid gene carrier in 293T cells and NIH-3T3 cells are the same as the gene transfection efficiencies of positive control lipofectamine of lipofectamine<TM>2000 in the 293T cells and the NIH-3T3 cells; the gene transfection efficiencies of the EGFR antibody modified cationic polymer lipid genetic carrier in liver cancer Huh-7 cells and breast cancer MCF-7 cells are higher than the gene transfection efficiencies of the lipofectamine<TM>2000 in the liver cancer Huh-7 cells and the breast cancer MCF-7 cells. The cationic polymer lipid genetic carrier system provided by the present invention has good biocompatibility and low cytotoxicity, and can be as an excellent non-viral gene delivery carrier.
Owner:SHANGHAI INST OF ONCOLOGY
Targeting drug/gene carriers to irradiated tissue
InactiveUS20050186264A1Low magnificationImmunoglobulins against cell receptors/antigens/surface-determinantsAntibody ingredientsMedicineLiposome
The present invention provides targeted delivery systems to deliver pharmaceuticals to irradiated tissue comprising a biomolecule carrier, a targeting moiety to cellular adhesion molecules and a pharmaceutical. The present invention also provides methods of selectively targeting endothelial tissue for delivery of a pharmaceutical thereto and of treating a pathophysiological state in an individual using the targeted delivery systems disclosed herein. Further provided is a method of optimizing an immunoliposome for specific targeting of a pharmaceutical encapsulated therein to irradiated tissue by selecting a liposome that has a greater rate of adhesion to the irradiated tissue than a rate of uptake by the reticuloendothelial system.
Owner:BOARD OF TRUSTEES OF OHIO UNIV THE +1
Anti CD19 engineered antibody for target conjugated lymphocyte, leuco cyte and its use
InactiveCN1775808AImmunoglobulins against cell receptors/antigens/surface-determinantsAntibody ingredientsTriturationGenetic engineering
The invention discloses an anti-CD19 engineering antibody and its application which is used in target direction combining lymphocytic leukemia cell. It lays a foundation for the next trituration genetic engineering medicine of the target direction therapy leukemia. It relates to anti-CD19 monoclonal antibody HI19a heavy and light chain variable region gene, and application of the gene code polypeptide, the gene carrier, and using the gene and polypeptide to make leukemia therapy medicine. The heavy and light chain variable region gene is come from the anti-CD19 monoclonal antibody HI19a. The invention successfully adopts gene engineering technique to make anti-CD19 gene engineering antibody, and lays a foundation for the target direction therapy of the leukemia.
Owner:INST OF HEMATOLOGY & BLOOD HOSPITAL CHINESE ACAD OF MEDICAL SCI
Nitrilase, encoding genes, carrier and application
InactiveCN104962540AAtom economy is highHigh potential for industrial applicationHydrolasesFermentationBrassica rapaBirdsrape Mustard
The invention discloses nitrilase from brassica rapa, encoding genes and an application of the encoding genes to the preparation of pregabalin chiral intermediate (S)-3-cyan-5-methylhexanoic acid through biocatalysis, and an amino acid sequence of the nitrilase is shown as SEQ ID NO:1. The invention provides novel nitrilase for preparing the (S)-3-cyan-5-methylhexanoic acid with high region and high stereoselectivity through hydrolysis, the concentration of hydrolysis substrates of the nitrilase can reach more than 1 M, and an ee (enantiomeric excess) value is kept more than 99 percent. The pregabalin important chiral intermediate (S)-3-cyan-5-methylhexanoic acid can be prepared through the nitrilase; a preparation method of the (S)-3-cyan-5-methylhexanoic acid has the advantages of high atom economy, mild conditions, environmental friendliness and the like and has high industrial application potential.
Owner:ZHEJIANG UNIV OF TECH
Low-molecular weight, water-soluble chitosan nanoparticle for gene delivery with folic acid conjugaed thereto as target ligand and preparation method thereof
InactiveUS20100040694A1Well formedImprove expression rateOrganic active ingredientsBiocideGene deliveryWater soluble chitosan
Disclosed are low-molecular weight, water-soluble chitosan nanoparticles with folic acid conjugated thereto as a target ligand and a preparation method thereof. The nanoparticles can be simply prepared since the strong reactivity of the chitosan allows folic acid to be readily introduced thereinto. Also, the folic acid-conjugated, low-molecular weight, water-soluble chitosan nanoparticles can be useful as gene carriers because they are of low or zero-toxicity, have sizes suitable for use as gene carriers, can readily form complexes with DNA, allow high gene expression rates, and are excellent in targeting tumor cells which are rich in folic acid receptors.
Owner:IND ACADEMY COOPERATION CORPS OF SUNCHON NAT UNIV 20
Fluorine-containing aliphatic chain-modified cationic polymer and application of fluorine-containing aliphatic chain-modified cationic polymer as gene carrier
ActiveCN104017828ALow priceEasy to synthesizeOther foreign material introduction processesVector-based foreign material introductionCationic polymerizationPolyamide
Owner:EAST CHINA NORMAL UNIV
Method for preparing glutathione response shell disulfide bond crosslinking non-virogene vector
InactiveCN101265477ASimple methodDifferent transfection efficienciesVector-based foreign material introductionHigh concentrationPlasmid dna
The invention discloses a method for preparing a glutathione-response shell disulfide bond crosslinking non-virus gene carrier. The method comprises the following steps: (1) synthesizing sulfhydrylation polyethyleneimine; (2) preparing sulfhydrylation polyethyleneimine aqueous solution with concentration of 0.1-1mg / ml; (3) preparing plasmid DNA solution with concentration of 50-250Mu g / ml; and (4) adding the solution prepared in step (2) to the solution of the same volume prepared in step (3), whirling to mix, standing to obtain sulfhydrylation gene carrier solution, stirring in air, and obtaining the glutathione-response shell disulfide bond crosslinking non-virus gene carrier. Through the preparation of the shell disulfide bond bionic crosslinking non-virus gene carrier, the stability of the gene carrier in physiological salt solution can be improved. The degradation of high concentration reduced glutathione in cells on disulfide bond can release associated DNA molecule so as to realize effective gene transfection.
Owner:ZHEJIANG UNIV
Detection kit for virulence gene of spinal muscular atrophy and application thereof
InactiveCN106319085AAccurate detectionEasy to detectMicrobiological testing/measurementSpinal muscular atrophiesNormal people
The invention provides a detection kit for virulence gene of spinal muscular atrophy and application thereof, comprising primer SMN-Ex7-F with such sequences as SEQ ID NO.1; primer SMN-Ex7-R with such sequences as SEQ ID NO.2 and probe SMN-Ex7-P with such sequences as SEQ ID NO.3. The above kit in the invention can be applied to detect the carrier of spinal muscular atrophy virulence gene, comprising the steps of extracting the genome DNA from subject to be detected; precisely quantifying the genome DNA from subject to be detected; and performing primary fluorescent quantitation PCR based on genome DNA from subject to be detected as the template to obtain the primary fluorescent quantitation curve. The kit in the invention can visually, precisely, quickly and succinctly detect the SMN1 gene, so as to realize the distinguishing of SMA virulence gene carrier and normal people.
Owner:THE FIRST AFFILIATED HOSPITAL OF ZHENGZHOU UNIV
Target type polypeptide nanometer gene carrier compound
ActiveCN106890343AAvoid clearingImprove targetingOrganic active ingredientsGenetic material ingredientsTumor targetNano structuring
The invention relates to the field of gene carriers, and particularly discloses a target type polypeptide nanometer gene carrier compound. The target type polypeptide nanometer gene carrier compound is prepared from a nano gene carrier with positive charges, a gene medicine and a tumor targeted antibody, wherein the nano gene carrier is adsorbed with the gene medicine and the tumor targeted antibody; and the nano gene carrier is prepared by coupling polypeptides with hydrophobic functional molecules. According to the target type polypeptide nanometer gene carrier compound, the nano gene carrier is formed by self-assembling and carries small interfering nucleic acid; the surface of the nano gene carrier is adsorbed with the tumor tissue targeted antibody; the nano gene carrier with the rich positive charges is used for adsorbing the targeted antibody with negative charges, so that the nano gene carrier with the positive charges can be cleared away in vivo circulation, and the targeting capacity can be also improved. The method is simple in process, mild in reaction conditions and easy to operate. Moreover, no covalent bonds are generated in the self-assembling process, so that no reverse reaction is caused, and highly ordered nano structures can be formed; and the target type polypeptide nanometer gene carrier compound is particularly suitable for constructing a biological medical material.
Owner:THE NAT CENT FOR NANOSCI & TECH NCNST OF CHINA
Bitarget fibroblast growth factor acceptor and transgene carrier of integrated element
InactiveCN1757738AGrowth inhibitionLow transfection efficiencyGenetic material ingredientsFermentationAbnormal tissue growthDisease
A transgenic carrier with deal targets (fibroblast growth factor receptor and integrant) is composed of the polypeptide CR16 specifically linked with alkaline fibroblast growth factor receptor, the polypeptide CP9 specifically linked with integrant, and the transgenic non-virus carrier system of CR16 / CP9 / cationic polymer PEI / exogenous DNA. Said carrier can effectively introduce the exogenous DNA to the tumor cell line and tumor tissue with high expression of FGFRs, so suppressing the growth of tumor. It can be used to preparation of the medicine for treating tumor and other diseases.
Owner:ZHEJIANG UNIV
Gene transduction method with nano granule as carrier based on ultrasonic intervention guiding
InactiveCN1687427ALow priceEasy to operateImmobilised enzymesOther foreign material introduction processesParticulatesCell wall
A gene transduction method ultrasonic-based, which using the nano particulate as the carrier. It consists of animal transgenic gene method and plant transgenic gene method. The outer gene joins with the particulate with the effect of static form together to form the gene carrier, which can protect the DNA being broken by ultrasonic. The ultrasonic synchronously give effect to particulate gene carrier, cells, organize and apparatus, the carrier can run efficiently through the short period alleyway of cell wall, cell film, core film cell, enables the outer gene constructs with the gene group in cell. This method has a high efficiency, and is provided with convenient manipulation, high efficiency, no request of differential function e.g. other merits. It can be applied widely to these range, animal and plant transgenic technology and genic cure of human being.
Owner:HUNAN UNIV
Polycationic gene carriers formed of endogenous amino group-bearing monomers
InactiveCN102083972AGenetic material ingredientsOther foreign material introduction processesCarbamateCarrier system
Owner:金拓
Phosphoryl choline chitosan derivative synthesis method
The invention discloses a synthesizing method of phosphocholine chitosan derivant, which comprises the following steps: reacting chitose powder and disubstituted Choline phosphonate with different proportions in the composite system of alkaline, carbon tetrachloride and secondary alcohol at 0-40 Deg C for 12-24h; hydrolyzing through alkaline liquid to obtain the product with different coupling rate. The invention modifies biological function and compatibility, which can be applied in the drug and gene carrier, tissue engineering rack, biological medical film, aquagel and biological intelligent instrument domain.
Owner:JINAN UNIVERSITY
Multi-copy gene carrier of high efficient expression mannanase
The present invention relates to gene engineering bacteria, and is especially one expression vector for high efficiency secreting expression on mannase gene and its multicopy body. Primer is designed according to the cloning site of Pichia yeast pAO815, the polyclonal site region of the target expression vector is replaced into Not I, XhoL I and EcoR I to form polyclonal sites for easy cloning, the alpha-factor signal peptide and the target gene are fused and connected to the expression vector for the expression vector containing the target gene to secrete and express mannase, and the restriction enzymes Bgl II and BamH I with complementary sticky ends are cleaved, linked and converted to obtain multicopy expression unit plasmid containing mannase gene. The multicopy body after transforming Pichia yeast can express mannase in high efficiency and is used to prepare mannase from konjaku meal.
Owner:广州伯凯生物技术有限公司
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