4983results about "Energy modified materials" patented technology

This present disclosure is directed to poly (ADP-ribose) polymerase (PARP) inhibitor nanoparticle composition and methods of use and preparation thereof. PARP inhibitor dose reduction through combination treatment may be achieved using photodynamic therapy or photoimmunotherapy.

A method of preparing sterile human placental allografts by providing a human placental tissue from a donor within 24 hours to 72 hours post-childbirth; removing any visible blood, blood clots, and / or blood components from the human placental tissue without scraping or scrubbing the human placental tissue to preserve structural integrity of the human placental tissue; washing the human placental tissue in an isotonic solution while maintaining the structural integrity of the human placental tissue; dehydrating the human placental tissue thereby forming the dehydrated human placental tissue; resizing the dehydrated human placental tissue into dehydrated human placental tissue portions having predetermined sizes; and sterilizing the dehydrated human placental tissue portions of step (e) thereby forming the sterile human placental allograft. Also disclosed is a sterile human placental allograft produced by the method.

A sterile micronized composition prepared from intact human placental tissue for wound healing. The sterile micronized composition includes micronized human amnion; micronized human chorion; and micronized human intermediate spongy layer. In certain aspects, the composition is prepared from intact placental tissue comprising a human amnion layer, human chorion layer, and an intact human intermediate spongy layer positioned between the human amnion layer and human chorion layer such that immediately before micronization the human amnion layer and the human chorion layer are not separated from one another.

The invention provides temperature and pH responsive hydrogel entrapped with chlorogenic acid lipidosome as well as a preparation method and application of the temperature and pH responsive hydrogel, and relates to the technical field of biology. The temperature and pH responsive hydrogel entrapping chlorogenic acid lipidosome contains chlorogenic acid lipidosome, phenylboronic acid grafted modified gelatin and dopamine grafted oxidized hyaluronic acid, the concentration of the phenylboronic acid grafted modified gelatin is 3-10 wt%, the concentration of the dopamine grafted oxidized hyaluronic acid is 3-10 wt%, and the concentration of chlorogenic acid is 1-20 [mu] M. The temperature and pH responsive hydrogel can effectively promote wound healing independently or in combination with blue light.

The invention relates to the technical field of nano material application. The invention provides a ruthenium-doped Prussian blue nano-enzyme and a preparation method thereof. The ruthenium-doped Prussian blue nano-enzyme is obtained by reacting metal ruthenium with Prussian blue nano-particles at normal temperature. The invention further provides a nano-enzyme library containing the ruthenium-doped Prussian blue nano-enzyme and a construction method of the nano-enzyme library. The invention also provides the SNP-PB nano-enzyme obtained by loading sodium nitroprusside on the ruthenium-doped Prussian blue nano-enzyme. The ruthenium-doped Prussian blue nano-enzyme and the SNP-PB nano-enzyme both have good antibacterial and anti-inflammatory performance, after NO gas release capacity is given, the problem of resistance of a biological membrane is better solved, then oxidative stress resistance and periodontal bacterial infection resistance are better exerted, and a new treatment method is provided for periodontal diseases.

The invention discloses a double-layer microneedle patch and a preparation method thereof.The preparation method of the double-layer microneedle patch comprises the following steps that firstly, 3D printing is conducted through polydimethylsiloxane, and a PDMS mold similar to a machining microneedle is obtained; dispersing the medicine into the sericin solution to serve as an outer layer solution; dispersing germanium nanoparticles into the polycaprolactone solution to obtain an inner layer solution; and finally, adding the outer-layer solution into the needle-shaped groove of the PDMS mold, so that the needle-shaped groove is filled with the outer-layer solution, drying, adding the inner-layer solution, drying, and demolding to obtain the double-layer microneedle patch. When the double-layer microneedle patch is used for drug delivery, drugs can be dissolved and released in a short time, the inner layer is polycaprolactone (PCL) carrying photo-thermal agent germanium nanoparticles, degradation is slow, a photo-thermal effect is generated under irradiation of 808nm near-infrared light, residual tumor cells are induced to die immunogenicity, an immune microenvironment is programmed in a time sequence, and the double-layer microneedle patch has the advantages of being high in sensitivity, high in sensitivity and good in stability. Postoperative recurrence and metastasis of tumors are efficiently inhibited, and meanwhile operative wound repair is promoted.

The invention provides an atomizing agent containing stem cell exosomes as well as a preparation method and application of the atomizing agent, and belongs to the technical field of exosomes. Ferroferric oxide magnetic particles are prepared, the surfaces of the ferroferric oxide magnetic particles are modified through tannic acid, berberine and adriamycin are loaded, then the ferroferric oxide magnetic particles and the exosome secreted by adipose-derived mesenchymal stem cells are mixed, incubated and centrifuged, the mixture is added into normal saline, uniform ultrasonic dispersion is conducted, and the atomizing agent containing the stem cell exosome is prepared. The prepared atomizing agent containing the stem cell exosome is simple in preparation method and wide in raw material source, the prepared atomizing agent directly enters the lung through spray administration, the unique performance of magnetic targeting drug delivery is achieved, drugs are released in a targeted and fixed-point mode, the drug effect is improved, and the effects of efficiently promoting tumor cell apoptosis and resisting lung cancer and pneumonia are achieved.

The invention discloses a porphyrin-based covalent organic framework nano material, a preparation method and application of the porphyrin-based covalent organic framework nano material in polyurethane, and belongs to the field of functional nano materials and antibacterial materials. The porphyrinyl covalent organic framework nano material is prepared from 5, 10, 15, 20-tetra (4-aminophenyl) porphyrin and bis-(5-formyl furfuryl) ether through a Schiff base reaction, and the structural formula of the porphyrinyl covalent organic framework nano material is # imgabs0. The porphyrinyl covalent organic framework nano material prepared by the invention has relatively high photothermal conversion efficiency when being used as a photothermal agent, and has a relatively good sterilization effect under the condition of relatively low concentration. Besides, based on the porphyrin-based covalent organic framework nano material, a WPUS / Por-COF composite film is prepared, the composite film has excellent mechanical properties and excellent photothermal conversion efficiency, and has a good sterilization effect under the condition of relatively low concentration, bacteria can be inactivated within a short time, and the sterilization rate can reach 99% or above.

The invention provides a composition for promoting hair regeneration after chemotherapy as well as a preparation method and application thereof, and belongs to the technical field of medicines. The preparation method comprises the following steps: modifying the surface of a gold nanocage with polydopamine, loading the modified gold nanocage on an exosome, complexing magnesium ions and zinc ions to prepare a modified exosome, preparing a nano drug-loaded liposome from curcumin, resveratrol and epigallocatechin gallate, mixing the nano drug-loaded liposome with the modified exosome to prepare a multi-layer liposome, and preparing the multi-layer liposome from the nano drug-loaded liposome. And mixing with a penetration enhancer, and adding into a temperature-sensitive hydrogel system to prepare the composition for promoting hair regeneration after chemotherapy. The composition for promoting hair regeneration after chemotherapy has a good transdermal absorption promoting effect, can slowly release drugs, promotes hair follicles to be converted from a resting stage to a growing stage under the synergistic effect of the drugs, regulates and controls multiple pathways (such as anti-apoptosis, angiogenesis promotion and anti-inflammation) at the same time, is matched with a complex pathological mechanism of hair regeneration after chemotherapy, and can promote hair regeneration after chemotherapy. Wide application prospects are realized.

The invention discloses a drug-loaded liposome nano-particle, a preparation method thereof and application of the drug-loaded liposome nano-particle in treatment of brain glioma, and belongs to the technical field of biological nano-medicines. The invention provides a novel copper phthalocyanine derivative and catalase co-loaded lipidosome nanoparticle and application of the novel copper phthalocyanine derivative and catalase co-loaded lipidosome nanoparticle to treatment of brain glioma on the cellular level. The nano-particles are obtained by wrapping copper phthalocyanine derivatives and catalase with lipidosome prepared by a microfluidic method. The co-drug-loaded liposome can catalyze excessive H2O2 in brain glioma to generate oxygen, and O2 is continuously supplied to photodynamic therapy (PDT) to generate active oxygen; meanwhile, Cu < 2 + > can deplete glutathione and improve the oxidative stress level in the glioma, the reduction product Cu < + > can convert H2O2 into hydroxyl radicals with higher toxicity through a Fenton-like reaction, oxidative stress injury is doubly amplified, cascade cooperation of PDT and chemical dynamic therapy (CDT) is achieved, and the treatment effect on the glioma is remarkably enhanced.

The invention provides a metal polyphenol network coated self-assembled nano-drug as well as a preparation method and application thereof, and belongs to the technical field of nano-drugs. The self-assembled nano-drug provided by the invention is of an organic-inorganic hybrid core-shell nano-structure; the core of the organic-inorganic hybrid core-shell nano structure is BC NPs; the BC NPs is formed by self-assembly of BBR (Boron Butadiene Rubber) and Cur (Carbon); a shell layer of the organic-inorganic hybrid core-shell nanostructure is a dynamic cross-linked network formed by self-assembly of metal ions and polyphenol through coordination. Metal ions and polyphenol are self-assembled through coordination to form a dynamic cross-linked network, and the dynamic cross-linked network coats the outer layer of the BC NPs nano-particles, so that the BC NPs nano-particles have good biocompatibility and biological safety; and the self-assembled nano-drug shows an obviously enhanced antibacterial effect under the combined treatment of NIR and H2O2.

The invention provides an aggregation-induced emission molecule, a nano motor and a preparation method and application of the aggregation-induced emission molecule, the aggregation-induced emission molecule is used for near-infrared emission, has photo-thermal properties, and has the characteristics of near-infrared II region luminescence, heat production and good light stability; the nano motor is prepared by taking an asymmetric double-mesoporous nano material with different pore diameters as a support material and selectively loading aggregation-induced emission molecules on one side of the support material with a larger pore diameter; under the irradiation of near-infrared light, the aggregation-induced emission molecules with the photothermal effect can form a non-uniform thermal gradient to push the self-thermophoresis movement of the nano motor; tumor sites can be diagnosed and reported under the mediation of near-infrared II region imaging; through the photo-thermal effect, the motor can destroy tumor interstitial cells and degrade tumor matrix components; and in combination with autothermophoresis, blood vessels can be penetrated, so that penetration to the deep layer of the tumor can be realized.

The invention provides a nano photosensitizer modified by outer membrane vesicles of genetically engineered bacteria as well as a preparation method and application of the nano photosensitizer. The nano photosensitizer is a nano material with the particle size of 80-120 nm, and the nano photosensitizer modified by the genetically engineered bacterium outer membrane vesicle comprises dipalmitic acid phosphatidylcholine (DPPC), distearoyl phosphatidylcholine (DSPC), dioleoyl lecithin (DOPC), cholesterol, a small molecule dye and CD47nb-OMV membrane protein. The chemical structure of the small molecule dye is shown as a formula I or a formula II. The nano photosensitizer OBDlp modified by outer membrane vesicles of genetically engineered bacteria serves as a novel nano photosensitizer, tumor targeting PTT is mediated through NIR II FL / PA imaging, tumor immunogenicity is improved, antigen presenting cells are activated, and infiltration and functionalization of CD8 + T cells are caused. And OBDlp and laser are combined to induce release of a large amount of DAMPs and trigger ICD to promote maturation of cDC1s. Meanwhile, a CD47 nano antibody carried by the nano photosensitizer induces polarization of M1 phenotype macrophages by blocking a CD47-SIRP alpha signal transduction axis, and an immunosuppression tumor microenvironment is reversed.

The invention discloses a multi-metal borate nano-particle as well as a preparation method and application thereof. The preparation method comprises the following steps: in the presence of small molecules with alkyl chains, performing high-temperature thermal decomposition on an organic metal compound or a metal salt compound precursor and organic borate in a solvent, and then centrifuging, modifying, performing ultrafiltration, dialyzing and purifying to obtain the multi-metal borate nanoparticles. The preparation method disclosed by the invention has the characteristics of mildness, high efficiency and strong controllability. The multi-metal borate nanoparticles prepared by the preparation method disclosed by the invention have the advantages of monodisperse size, relatively high magnetic property and good biocompatibility. Therefore, the prepared multi-metal borate nanoparticles not only have multi-modal imaging functions such as magnetic resonance imaging, near-infrared two-region imaging, nuclear medicine imaging and magnetic particle imaging, but also can be used for various treatment means such as magnetocaloric therapy and boron neutron capture therapy; potential advantages are realized in biomedical applications such as magnetic targeting tumor treatment and imaging contrast agents, and high practical values are realized.

The present disclosure relates to methods that enable the continuous formation of droplets and dehydration of droplets to provide pharmaceutically relevant particles that can be used for therapy. In particular, the methods disclosed herein allow the controlled continuous droplet formation and dehydration that produce circular particles having low internal void spaces comprising bioactive therapeutic biologies.

The invention provides a novel organic conjugated molecule, the long conjugated D-pi-A structure enables the organic conjugated molecule to form nanoparticles with high positive charge density more easily, and meanwhile, a large amount of active oxygen generated under the irradiation of white light can efficiently split bacterial cell membranes and slow down the generation of bacterial drug resistance. The organic conjugated molecule as shown in the formula (I) has a strong photodynamic killing effect on gram-negative bacteria and gram-positive bacteria, and is low in hemolysis rate and good in biocompatibility. The invention provides guidance for developing a high-efficiency and low-toxicity photodynamic antibacterial agent.

The invention discloses a macrocyclic covalent organic polymer material for inhibiting a pi-pi stacking effect and application of the macrocyclic covalent organic polymer material, and belongs to the technical field of biological medicines. The preparation method comprises the following steps: adding 5, 10, 15, 20-tetra (4-ethynylphenyl) copper porphyrin and a macrocyclic monomer into an organic solvent in a protective atmosphere, and carrying out solvothermal reaction in the presence of a catalyst and alkali to obtain the macrocyclic covalent organic polymer material. According to the invention, 5, 10, 15, 20-tetra (4-ethynylphenyl) copper porphyrin and a macrocyclic monomer form a porous polymer MCP-CU-POP, the material utilizes the photo-thermal capability to combine the effects of simulated photo-thermal (PTT), photodynamic (PDT), peroxidase (PODs) and glutathione peroxidase (GSH-Px) to perform synergistic antibiosis, and the inherent characteristics of an infected microenvironment can also be utilized to induce death of bacteria, so that the antibacterial property of the material is greatly improved, and the antibacterial property of the material is greatly improved. And the microenvironment of the bacterial infection part can be adjusted, so that the recovery of the bacterial infection wound can be obviously accelerated.

The invention discloses a nano-antibacterial agent for treating biofilm infection through Prussian blue and a preparation method and application thereof.According to the nano-antibacterial agent, hollow Prussian blue (HPB) serves as a carrier to load a substance with anti-inflammatory and anti-oxidation effects, a metal polyphenol network structure is formed through chelating of tannic acid (TA) modified by hyaluronic acid (HA) and high-valence metal ions, and the nano-antibacterial agent is used for treating biofilm infection through Prussian blue. And the MPNs are packaged to obtain the product. All the components of the nano antibacterial agent have a synergistic effect, MPNs formed by chelating TA modified by hyaluronic acid and high-valence metal ions in the antibacterial agent have good photo-thermal performance, and light energy can be converted into heat energy for photo-thermal therapy. According to the invention, the nanoparticles for photo-thermal antisepsis and anti-inflammation are constructed, so that comprehensive treatment of anti-biofilm and anti-inflammatory effect is realized, and healing of infected parts is promoted. And the antibacterial agent has good biocompatibility and high biological safety, and is very suitable for replacing antibiotics to treat biological membrane infection.

The invention discloses multifunctional hydrogel for promoting healing of diabetic wounds and a preparation method of the multifunctional hydrogel. The multifunctional hydrogel is prepared from Ti3C2Tx-Ag, paeoniflorin (PF), a gel matrix oxidized konjac glucomannan (OKGM) solution and carboxymethyl chitosan (CMCS). The method comprises the following steps: mixing Ti3C2Tx powder with AgNO3 in deionized water, heating to boiling, adding a proper amount of a sodium citrate solution, reacting to obtain Ti3C2Tx-Ag (TA) powder, and mixing the powder with a paeoniflorin (PF) and oxidized konjac glucomannan (OKGM) solution and a carboxymethyl chitosan (CMCS) solution to prepare the KC-PF-TA hydrogel. The multifunctional hydrogel has excellent photo-thermal antibacterial performance, anti-inflammatory performance and angiogenesis promoting performance, shows a remarkable effect of promoting healing of diabetic wounds through the synergistic effect of Ti3C2Tx-Ag and paeoniflorin, and has good biocompatibility; the multifunctional nano-composite hydrogel disclosed by the invention has great application prospect and value in the field of wound repair materials.

The invention belongs to the technical field of micro-nano, and particularly relates to a drug-loaded microalgae motor and a preparation method and application thereof, and the drug-loaded microalgae motor comprises a polydopamine / ferroferric oxide / chlamydomonas reinhardtii micromotor and a drug loaded on the polydopamine / ferroferric oxide / chlamydomonas reinhardtii micromotor, the drug is combined with catechol groups on the surface of polydopamine in the polydopamine / ferroferric oxide / chlamydomonas reinhardtii micromotor through covalent bonds; the medicine is an anti-cancer medicine or an anti-inflammatory medicine. The microalgae motor has the characteristics of simple preparation method, good material biocompatibility, high biodegradability and the like. The polydopamine / ferroferric oxide / chlamydomonas reinhardtii drug-loaded micromotor prepared by the invention has the characteristic of self-driving in intestinal juice, so that the polydopamine / ferroferric oxide / chlamydomonas reinhardtii drug-loaded micromotor can target an intestinal disease part under the guidance of an external magnetic field to improve the treatment effect and reduce the side effect of a drug, thereby expanding the application space of a micro-nano motor in the field of biomedicine.

The invention relates to the technical field of nano-drugs, in particular to an acoustic / chemical power synergistic anti-tumor nano-drug as well as a preparation method and application thereof. The invention provides an acoustic / chemical power synergistic anti-tumor nano-drug, which takes hollow mesoporous MnO2 nano-particles as a carrier, and is loaded with vitamin C and a sound-sensitive agent chlorin e6. Vc can generate abundant H2O2 to provide a raw material for CDT, and MnO2 is used as a peroxidase-like enzyme to catalyze H2O2 to generate O2, so that tumor hypoxia is effectively relieved, and the effect of sonodynamic therapy is enhanced. Besides, GSH induces MnO2 degradation, GSH consumption is caused to enhance CDT, GSH depletion can cause glutathione peroxidase 4 (GPX4) activity reduction, ROS generation and GPX4 activity reduction have a synergistic effect, lipid peroxides (LPOs) are increased, and finally ferroptosis of tumor cells is caused.

The invention provides lipidosome wrapping L-arginine / Prussian blue nano particles and a preparation method and application thereof, and belongs to the technical field of biological agents. L-arginine is deposited on hPB through iron chelation, then the lipidosome is coated to avoid leakage of water-soluble L-arginine in circulation, and thus the L-arginine / Prussian blue nano particles are obtained. The liposome coated with the L-arginine / Prussian blue nano particles is obtained. Due to the EPR effect, the hPLL-coated Lipo can be efficiently transferred and distributed into tumor tissues, heat is generated under the near-infrared light irradiation condition to ablate a large number of tumor cells, the utilization degree of the tumor cells to L-arginine is greatly limited, meanwhile, immunogenic death is induced, DAMPS is released in situ, infiltration and activation of anti-tumor T cells are further promoted, and the anti-tumor effect is achieved. The tumor immunotherapy is enhanced. And under illumination, the liposome disintegrates and releases the L-arginine, so that nutritional support is provided for immunological competence cells in competitive disadvantage, and activation of an immune microenvironment is promoted.

The invention belongs to the technical field of biomedical materials, and discloses injectable heterogeneous cascade nano-enzyme antibacterial hydrogel as well as a preparation method and application thereof. The preparation method comprises the following steps: in a process of preparing cerium dioxide nano-enzyme by a reverse micelle method, introducing molybdenum disulfide nano-particles with a nano flower-shaped structure so as to deposit the cerium dioxide nano-enzyme on the surfaces of the molybdenum disulfide nano-particles in situ to obtain nano-composite particles; through electrostatic interaction, glucose oxidase is loaded on the surfaces of the nano composite particles to form a heterogeneous cascade nano enzyme material; the preparation method comprises the following steps: uniformly mixing sodium alginate and hyaluronic acid in water, and sequentially adding an NO donor, a heterogeneous cascade nano-enzyme material and a cross-linking agent to obtain the injectable heterogeneous cascade nano-enzyme antibacterial hydrogel. The preparation method is simple, and the injectable heterogeneous cascade nano-enzyme antibacterial hydrogel has a strong antibacterial effect and can be used for preparing the antibacterial hydrogel. Particularly, the compound has a remarkable killing effect on gram-negative bacteria and gram-positive bacteria.

The invention discloses a double-layer gel patch for diabetic wounds and a preparation method of the double-layer gel patch. The double-layer gel patch is composed of an upper gel layer and a lower gel layer, and when the double-layer gel patch is used, the lower gel layer is attached to the surface of skin; the upper gel layer is prepared from methacrylated gelatin, oxidized hyaluronic acid, a photoinitiator and an anti-inflammatory drug, and the lower gel layer is prepared from 3-carboxyphenylboronic acid grafted gelatin, dihydrocaffeic acid grafted epsilon-polylysine and an antibacterial agent. The double-layer gel disclosed by the invention has high mechanical strength and absorbability, and meanwhile, a sequential treatment strategy of firstly resisting bacteria and then resisting inflammation can be realized.

The invention discloses a dual-mechanism antibacterial photosensitizer as well as a preparation method and application thereof, and relates to the technical field of biomedical photoelectric functional materials. The invention relates to a triphenylamine derivative, which can realize fluorescence imaging of bacterial or fungal infection by utilizing fluorescence characteristics, can generate active oxygen under illumination to play a photodynamic therapy role, is suitable for preparing photodynamic therapy preparations and developing antibacterial materials, and can also be applied to the fields of environment and public health and tumor treatment. The problems that an existing photosensitizer is insufficient in broad spectrum, different in fungus drug resistance mechanism, limited in single treatment effect and the like can be effectively solved, and the photosensitizer has wide application prospects.

A magnetite-based micro / nanorobot and a preparation method and use thereof are provided. The magnetite-based micro / nanorobot uses a polydopamine-coated magnetite as a carrier that is loaded with an anti-inflammatory drug and is finally coated with sodium alginate. The magnetite-based micro / nanorobot of the present disclosure improves a loading effect of the drug resveratrol and has an excellent anti-inflammatory effect. The magnetite-based micro / nanorobot of the present disclosure does not have obvious cytotoxicity, and exhibits excellent biocompatibility and high safety performance. The magnetite-based micro / nanorobot of the present disclosure allows the responsive release according to different pH values of a gastrointestinal environment. Compared with the traditional drug carriers, the magnetite-based micro / nanorobot of the present disclosure has a high rate and prominent targetability, and can be prepared by a method involving simple steps and easy operations.

The invention discloses a nano-silver intercalation covalent organic frame polymer and application thereof, and belongs to the technical field of biological medicine. The nano-silver intercalation covalent organic frame polymer PP-COF-Ag is a porous material which is formed by taking 4, 5-dihydropyrene-4, 5-diketone and tetraaminophenyl porphyrin as structural units and stacking lamellae, and the nano-silver intercalation covalent organic frame polymer PP-COF-Ag is a nano-silver intercalation covalent organic frame polymer PP-COF-Ag. And silver nanoparticles are inserted into the lamellae of the nano-silver intercalation covalent organic frame polymer. The preparation method comprises the following steps: enabling 4, 5-dihydropyrene-4, 5-diketone and tetraaminophenyl porphyrin to react to obtain black powder PP-COF; the PP-COF reacts with a silver nitrate solution, then a sodium borohydride methanol solution is dropwise added to reduce silver, and the PP-COF-Ag is obtained. The insertion of the nano-silver increases the interlayer spacing of the original covalent organic framework, and the nano-silver, photo-thermal therapy and photodynamic therapy can be combined to resist bacteria, and the nano-silver covalent organic framework is good in biocompatibility and free of toxic and side effects.

The invention relates to a two-dimensional nano heterojunction with space-time antibacterial and anti-inflammatory functions and a preparation method and application thereof, and belongs to the field of biomedical materials. The preparation method comprises the following steps: firstly, generating a BiCuSeO nanosheet, and resuspending the BiCuSeO nanosheet by using deionized water; the preparation method comprises the following steps: adding cerium nitrate hexahydrate into ethylene glycol, mixing with a BiCuSeO nanosheet resuspended by deionized water, and preparing the two-dimensional nano heterojunction with space-time antibacterial and anti-inflammatory functions by taking the BiCuSeO nanosheet as a seed and the cerium nitrate hexahydrate as a raw material through a hydrothermal synthesis method. Compared with the prior art, good photo-thermal performance of the heterojunction can be utilized, near infrared is used as an external field excitation source to regulate and control the temperature difference and excite the thermoelectric performance of the heterojunction, collaborative treatment of the photo-thermal performance and the thermoelectric performance is achieved, the enhanced anti-inflammatory function of the heterojunction is combined, and the heterojunction becomes a potential application material for infectious bone defects. The preparation method is simple, stable in condition and high in repeatability.

The invention relates to a core-shell structure drug-loaded magnetic-thermal hydrogel microsphere for treating drug-resistant ovarian cancer and a preparation method thereof, belonging to the field of biological and medical micro-nano materials and technologies, and the core-shell structure drug-loaded magnetic-thermal hydrogel microsphere is formed by combining an inner core and a shell layer through covalent crosslinking and electrostatic interaction. An anti-tumor drug cis-platinum and magnetic nanoparticles are loaded on an inner core, and an AZ191 inhibitor is loaded on a chitosan shell, so that preferential release of AZ191 is realized to inhibit a drug-resistant signal channel and reduce the drug resistance of cancer cells, and the cis-platinum is slowly released after the action is completed to kill tumor cells. And the magnetic nanoparticles in the inner core can be heated under an external magnetic field, so that multifunctional synergistic treatment of magnetic hyperthermia, drug resistance inhibition and chemotherapy is realized. Chitosan and sodium alginate are biodegradable materials, so that the utilization rate of the embedded medicine can be increased, and meanwhile, the medicine can be metabolized out of a body. The preparation method does not relate to toxic cross-linking agents and organic solvents, and the prepared hydrogel core-shell microspheres have no biotoxicity. Complicated operation steps are not needed, the technological process is simple, the preparation period is short, and the application range is wide.

The invention discloses an OTX-015 (Ox-015) loaded cascaded responsive nano particle and a preparation method thereof. The nanoparticle has a three-layer core-shell structure, wherein the innermost layer is a cross-linked nanoparticle of chitosan and paclitaxel; the middle layer is a polydopamine layer loaded with a bromodomain inhibitor OTX-015; the outermost layer is a gelatin layer grafted with babali; wherein the cross-linked substance of the chitosan and the paclitaxel is a cross-linked substance containing a disulfide bond. The invention also provides a preparation method of the cascade responsive nano-particle and application of the cascade responsive nano-particle in preparation of an antitumor drug targeted delivery system. The nanoparticle provided by the invention improves the tumor microenvironment while activating in-vivo autoimmunity, and kills tumor cells by using photothermal therapy assisted chemotherapy drugs, so that the synergistic effect of chemotherapy / photothermal therapy / immunotherapy is realized, and a new idea is provided for clinical tumor treatment.