138 results about "Phosphatidylcholine" patented technology

The application relates to the technical field of cough-relieving medicines, and discloses a high-efficiency cough-relieving loquat particle composition which is composed of the following components in parts by weight: loquat leaf extract 30-40 parts; white front extract 10-15 parts; platycodon grandiflorum extract 6-10 parts; white mulberry bark extract 18-25 parts; stemona sessiliflora extract 7-10 parts; menthol 0.1-0.5 part; polyethylene glycol 2-5 parts; mannitol 8-12 parts; ethyl cellulose 3-6 parts; hydroxypropyl methyl cellulose 1-3 parts; phosphatidylcholine 1-3 parts; polysorbate 80 1-3 parts; cross-linked sodium carboxymethyl cellulose 4-6 parts; and sucrose 40-60 parts. The double dispersion system combining the immediate-release component and the sustained-release component is adopted, the solubility and the initial release speed of the traditional Chinese medicine extract are remarkably improved through the addition of polyethylene glycol and mannitol, and the technical effect that the drug effect appearing time is remarkably shortened is achieved. Compared with the technical scheme relying on only a single release mode in the prior art, the deficiency that the drug effect is slow and the symptoms of the patient cannot be timely relieved is solved.

The invention relates to the technical field of biotechnology and medical detection, in particular to an ovarian cancer early-stage accurate screening method based on triple lipid metabolite chemical crosslinking, which comprises the following steps: sample collection: collecting a serum sample of a person to be detected; lipid metabolite extraction: extracting lipid metabolite from the serum sample; detection of triple lipid metabolites: simultaneously detecting the contents of sphingomyelin (SM), phosphatidylcholine (PC) and cholesteryl ester (CE) in the lipid metabolites by adopting a liquid chromatography-mass spectrometry technology, and by simultaneously detecting the three lipid metabolites of sphingomyelin (SM), phosphatidylcholine (PC) and cholesteryl ester (CE) and carrying out chemical cross-linking analysis, the content of the triple lipid metabolites in the lipid metabolites can be detected; and calculating the triple lipid metabolite index (TLMI), so that the abnormal lipid metabolism condition of the ovarian cancer patient can be more comprehensively and accurately reflected, and the sensitivity and the specificity of early screening of the ovarian cancer are improved.

The invention relates to a pharmaceutical composition consisting of a liposomal formulation of a phospholipid component and an aqueous phase, wherein the phospholipid component is selected from the group consisting of phosphatidylcholine, and sphingomyelin, and wherein the phospholipid component comprises a saturated or mono-unsaturated (Z / cis) fatty acid having 14 to 20 carbon atoms. The invention further relates to the use of the pharmaceutical composition in treatment of a condition associated with presence of lipopolysaccharide (LPS), particularly endotoxemia.

The application belongs to the technical field of anti-aging skin care product preparation, and discloses a composition containing a composite exosome-polypeptide, a preparation method thereof and application of the composition in cosmetics. The composition contains, in terms of mass fraction, 3.0%-7.0% of an exosome-polypeptide composite wrapped by a biomimetic liposome, 4%-9% of a plant active synergistic extract, 15%-20% of a temperature-sensitive moisturizing sustained-release system, 3%-6% of a lipid barrier repair agent, 0.3%-0.8% of an antioxidant stabilizer, and the rest is a basic base; the exosome is composed of membrane pod huangqi root exosome, salvia miltiorrhiza exosome and long life flower exosome; the polypeptide is composed of palmitoyl tripeptide-5 and acetyl hexapeptide-8; and the biomimetic liposome is composed of phosphatidylcholine, ceramide NP and phytosteryl oleate. The application is compounded by the composite exosome-polypeptide-multiple effect component, hierarchical synergy is achieved, multiple-dimensional aging targets are covered, the transdermal rate is improved, the activity is retained for a long time, the barrier repair and skin quality adaptation are considered, and the anti-aging performance is excellent.

The invention relates to a high-absorptivity blood fat reducing composition containing a burdock root water extract as well as a preparation method and application thereof, and relates to the field of preparation of blood fat reducing compositions. The blood fat reducing composition is prepared from burdock root aqueous extract, hawthorn flavone, cassia seed glycoside and phosphatidylcholine according to the mass ratio of (6 to 10): (1 to 2): (0.8 to 1.5): (0.5 to 1). According to the composition, the burdock root aqueous extract is taken as a core, hawthorn flavone, cassia seed glycoside and phosphatidylcholine are matched, and the problems of low absorption rate and single curative effect of the existing traditional Chinese medicine lipid-lowering medicine are solved through the dual design that active ingredients synergistically lower lipid and absorption-promoting ingredients improve bioavailability.

The invention discloses a compound grease feed additive for improving breeding quality of channa maculata and application. The method comprises the following steps: (1) preparing experimental feed; the method comprises the following steps: respectively preparing an experimental group ARA 1.5 added with 1.5% of arachidonic acid and an experimental group ARA + PC added with 1.5% of arachidonic acid and 1.5% of phosphatidylcholine; (2) feeding; (3) after the culture experiment is finished, treating and detecting the channa maculata; (4) carrying out an artificial propagation test; (5) carrying out ovarian steroid synthesis related gene expression detection; and (6) carrying out data statistical analysis. By adding arachidonic acid (ARA) and phosphatidylcholine (PC) into the feed, the breeding performance of channa maculata and the quality of fry are improved, and the feed has very important significance for improving the aquatic product efficiency of high-quality fry of channa maculata in aquaculture.

The present invention provides a nutritional composition comprising milk phospholipids and egg phospholipids, particularly suitable as a nutritional composition for infants, also as a nutritional support product for pregnant women and as a nutritional supplement for the elderly, in which phosphatidylcholine and sphingomyelin and hence choline are also highly correlated. The invention provides a nutritional composition containing choline with high bioavailability.

The application provides a nanoemulsion and a preparation method and application thereof. The total amount of raw materials for preparing the nanoemulsion is 100%, and the raw materials include the following components: 1-40% of a composite surfactant, 1-25% of an oil phase, 1-30% of a co-surfactant, and the balance of a water phase; wherein the mass of the composite surfactant is 100%, and the composite surfactant includes the following components: 5-25% of a biological surfactant, 35-65% of a fatty alcohol polyoxyethylene ether surfactant, 1-20% of dodecyl benzene sulfonic acid and / or sodium salt thereof, 1-20% of oleic acid, and 1-5% of citric acid; the biological surfactant includes one or more than two combinations of sophorolipid, rhamnolipid, trehalose lipid, surfactin, alkyl polyglycoside, phosphatidylcholine, amino acid lipid, saponin, phospholipid, or derivatives thereof; and the average particle size of the emulsion droplets in the nanoemulsion is 1.5-5 nm. The nanoemulsion has excellent recovery performance.

This invention discloses a soothing and repairing composition and its application, comprising the following raw materials: phytosterols, chamomile extract, hydrolyzed sodium hyaluronate, tocopherol, ceramide NP, triethanolamine, lactobacillus, macadamia seed oil, magnolia alcohol, matricaria flower extract, maltodextrin, lecithin, phosphatidylcholine, ascorbyl palmitate, inulin root juice, arginine / lysine polypeptide, squalane, jojoba seed oil, scutellaria baicalensis root extract, polygonum cuspidatum root extract, fructose, glycerin, p-hydroxyacetophenone, butylene glycol, acrylate / C10-30 alkanol acrylate crosspolymer, α-glucan oligosaccharide, 1,2-hexanediol, and water as the balance. Compared with the prior art, the soothing and repairing composition prepared by this invention has the effects of rapidly rebuilding the skin barrier, regulating skin immune function, improving skin microecology, synergistic effect, and rapid skin repair.

The invention discloses a cell membrane vesicle self-assembly body loaded with spirulina PDRN and a preparation method of the cell membrane vesicle self-assembly body, and belongs to the technical field of cosmetics. The preparation method comprises the following steps: homogenizing and crushing spirulina at low temperature and high pressure, and performing graded filtration to prepare spirulina vesicle suspension; mixing with auxiliary materials such as spirulina platensis source PDRN, hydrogenated soybean phosphatidylcholine and the like to prepare a medicine-carrying vesicle premixed solution; and carrying out pre-homogenization, low-temperature fine homogenization and graded filtration to obtain the target self-assembly body. According to the invention, the spirulina natural cell membrane vesicles are taken as a core carrier, efficient encapsulation of PDRN is realized through membrane fusion recombination, the encapsulation efficiency of the obtained product can reach 55% or more, the transdermal absorption performance is excellent, UVB-induced skin light injury cells can be remarkably repaired, and the product has the effects of resisting aging, tightening, resisting wrinkles and whitening, and can be widely applied to preparation of skin care products.

The invention provides powdery phospholipid with good free-running property and rich phosphatidylcholine as well as a preparation method and application of the powdery phospholipid, and belongs to the technical field of deep processing of phospholipid. According to the method, the crude phospholipid is sequentially subjected to absolute ethyl alcohol purification and acetone refining, the refining frequency is controlled, it is guaranteed that the PC content is 40 wt% or above, meanwhile, the isomaltitol is added in the last time of refining, and the isomaltitol serves as sugar alcohol which is low in hygroscopicity and easy to crystallize, so that the purity of the product is greatly improved. At the moment, a special co-dispersion system which can form with phospholipid in an acetone medium is added, and in the subsequent drying process, the phospholipid particles can be accurately filled and an isolation layer is formed, so that the physical structure of the product is fundamentally changed, and particle adhesion is prevented from internal anti-caking and internal isomaltitol is used as a skeleton; an anti-caking agent is arranged outside to serve as a'ball 'to further reduce friction, and the dual-action mechanism ensures that the final product has extremely excellent flowability and long-term storage stability.

The invention provides a phospholipid composition and an edible oil anhydrous degumming method. The phospholipid composition is separated from cakes or residues obtained by squeezing oil materials to prepare oil, the mass of phospholipid accounts for 70-100% of the total mass of the phospholipid composition, and the mass of phosphatidylcholine accounts for 25% or below of the total mass of the phospholipid composition. According to the degumming method, the phospholipid composition is used for degumming. By using the degumming method disclosed by the invention, the phosphorus content in the edible oil can be reduced, and the flavor is not influenced.

The application belongs to the technical field of cosmetics, and discloses an enzyme moisturizing composition, a preparation method and application thereof, and aims to solve the problem that the existing enzyme-containing mask is limited in enzyme efficacy due to skin residual grease blocking. The enzyme moisturizing composition comprises a yeast / rice fermentation product filtrate, an amphiphilic compound (consisting of phosphatidylcholine, PEG-10 stearate and lipase), sodium hyaluronate and the like; the mask has a face a and a face b, the face a is coated with the enzyme moisturizing composition, and the face b is coated with a supplementary moisturizing composition containing trehalose; step-by-step drying is adopted in the mask preparation, and the water content of each face of the mask is controlled to be 20-25%. The application can break through the grease blocking, fully exert the moisturizing and repairing efficacy of the yeast / rice fermentation product filtrate, and the mask is stable and has good skin feel.

The invention relates to the technical field of ultraviolet light analysis, and particularly discloses a qualitative and quantitative method of phosphatidylcholine, which is used for solving the problems that phosphatidylcholine multi-carbon-chain components are difficult to stably separate, retention drift is caused by different batches of mobile phase A solvent strength and selectivity fluctuation, integral mismatch is caused by acromion and co-elution, and the stability of the components is poor. Different carbon chain reference substances are difficult to prepare, so that qualitative and quantitative deviation is caused. The method comprises the following steps: preparing a test solution, performing HPLC gradient separation and mobile phase A closed-loop calibration, performing marker time axis correction and acromion MS resolution quantification, and performing QTOF-MS molecular weight qualitative and qualitative and quantitative contrast output. A mobile phase A is calibrated in a closed-loop manner through a marker alpha to stabilize the strength and selectivity of a batch of solvents, correction is performed according to a marker time axis, an integral window is generated automatically, a UV area is split according to an acromion EIC proportion to inhibit misintegration caused by drifting and peak stacking, and qualitative and quantitative detection of a to-be-detected sample is achieved without using a whole set of multi-carbon-chain PC reference substances.

A cyclocurcumin-based composition consisting of: a cyclocurcumin component present at 20 to 40 wt% of the total composition; a curcuminoid component present at 30 to 50 wt%, comprising 20 to 30 wt% curcumin, 5 to 10 wt% demethoxycurcumin, and 3 to 8 wt% bisdemethoxycurcumin; a solubilizer component present at 5 to 15 wt%, comprising polysorbate at 3 to 8 wt% and lecithin at 3 to 7 wt%; a complexing component present at 5 to 12 wt%, comprising cyclodextrin inclusion complexes; and a nanoparticulate component present at 3 to 10 wt%, comprising cyclocurcumin particles with a particle size in the range of 50 nm to 200 nm. consists of a polymer stabilizing component, which is present in an amount of 4 to 10 wt.-% is present and comprises polyvinylpyrrolidone or polyethylene glycol, a phospholipid complex component present in an amount of 3 to 8 wt% and consisting of phosphatidylcholine complexes, and an encapsulation component present in an amount of 2 to 6 wt% and containing maltodextrin or gum arabic, the composition being configured to ensure improved solubility, stability and bioavailability.

The invention relates to chlorotoxin-melittin nanoparticles as well as a preparation method and application thereof. The method comprises the following steps: weighing chlorotoxin, dissolving the chlorotoxin in a PBS buffer solution, adding 2-imino sulfane hydrochloride, fully mixing, and incubating at room temperature to form a CTX-SH solution; the preparation method comprises the following steps: weighing dimyristoyl phosphatidylcholine, adding a trichloromethane solution, uniformly mixing, then adding a cholesterol oleate solution and a phosphatidyl ethanolamine-polyethylene glycol 2000-maleimide solution, and sufficiently and uniformly mixing; drying the solution, resuspending with a PBS (Phosphate Buffer Solution), and fully dissolving by ultrasonic; adding a thiolated CTX-SH solution into the solution, incubating for 1-3 days, and carrying out ultrafiltration purification to form a CTX-NP nanoparticle solution; preparing a melittin solution, dropwise adding the melittin solution into the CTX-NP nanoparticle solution, incubating for 10-24 hours, and performing ultrafiltration purification. The invention has the advantages of targeting brain glioma, inhibiting the growth of in-situ brain glioma and improving the immunosuppressive microenvironment of brain glioma.

The present application relates to a kind of phospholipid choline structural compounds and its preparation method, the preparation method of the phospholipid choline structural compounds includes the following steps: with hydroxyethyl methacrylate and beta-mercaptoethylamine as raw material, intermediate M1 is obtained by reaction at room temperature;Intermediates M1 and acrylate are added to obtain intermediate M2;Intermediate M2 and 2-chloro-2-oxygen-1,3,2-dioxaphospholane are reacted to obtain intermediate M3;Then intermediate M3 and chain or cyclic tertiary amine are reacted to obtain phospholipid choline compound.The phospholipid choline compound obtained by the method of the present application is simple, mild, can be combined with many compound fragments on the market, and the product is rich and varied.Such phospholipid choline compound can be selectively delivered to the spleen site for the delivery of nucleic acid drugs.

The invention relates to the technical field of anti-tumor bionic nanomaterials, and discloses a preparation method and application of tumor-bacterial membrane chimeric nanoparticles, and the preparation method comprises the following steps: carrying out rotary evaporation on dimyristoyl phosphatidylcholine and cholesterol oleate to obtain an HDL rotary evaporation film; mixing the tumor cell membrane with the bacterial membrane to obtain a tumor-bacterial mixed membrane; pre-mixing apolipoprotein ApoA-1 with the tumor-bacteria mixed membrane, and adding the mixed solution into a container containing an HDL rotary evaporation membrane for hydration and reconstruction to obtain a crude suspension; and carrying out ultrafiltration purification on the crude suspension to obtain the tumor-bacterial membrane chimeric nanoparticles. The tumor cell membrane and the bacterial membrane are co-loaded on the high-density lipoprotein, so that the two membrane components can be co-delivered to the dendritic cells to induce maturation of the dendritic cells, are accumulated in tumors and drainage lymph nodes in vivo, activate CD8 + T cell response and have an inhibition effect on tumor growth.

The present invention provides a nutritional composition especially well suited as an infant nutritional composition, but also as a nutritional support product for pregnant women and as senior nutritional supplement, where phosphatidylcholine and sphingomyelin, and thus choline, also are highly relevant. The present invention provides a nutritional composition which contains choline of high bio-availability.

The invention belongs to the technical field of skin care products, and provides an anti-skin-aging bovine-derived protein peptide composition and a preparation method thereof. The preparation method comprises the following steps: preparing bovine-derived protein peptide with the molecular weight of 300-800Da by adopting a sequential enzymolysis technology, directionally enriching active peptide fragments with arginine and lysine content of more than or equal to 18% through cation exchange chromatography, constructing an antioxidant synergistic network with ascorbyl sodium phosphate, tocopheryl acetate and phytic acid, preparing a bionic liposome carrier by utilizing phosphatidylcholine, cholesterol and ceramide 3, and preparing the bovine-derived protein peptide biomimetic liposome. The surface of a carrier is sequentially coated with a poly L-histidine layer, a hyaluronic acid layer and a polycaprolactone outer layer by adopting a multi-layer response coating design, and is grafted with hyaluronic acid oligosaccharide to form a stable multi-layer structure. The average particle size of the prepared composition is 120-280 nm, the drug loading efficiency is larger than or equal to 82%, the 24-hour cumulative release rate is 58-82%, and the composition shows excellent controlled release performance and anti-aging activity and is suitable for development and application of high-end anti-aging skin care products.

The application belongs to the technical field of pesticide preparation, and particularly relates to 1-methylcyclopropene oil suspending agent and a preparation method thereof. The cyclodextrin inclusion compound of 1-methylcyclopropene is dispersed in a non-water medium, so that the rapid release of the cyclodextrin inclusion compound of 1-methylcyclopropene caused by water is avoided, the stability of the 1-methylcyclopropene oil suspending agent is improved, and meanwhile, the phosphatidylcholine is used in combination with an emulsifier, a thickening agent and other auxiliaries, so that the problem of high viscosity caused by the dispersion of cyclodextrin in an oil medium is solved, the appropriate viscosity of the 1-methylcyclopropene oil suspending agent is ensured, the use and storage are facilitated, the environment is friendly, and the 1-methylcyclopropene oil suspending agent can be used in open space and closed space at the same time.

The invention relates to tricholoma matsutake endophytic fungus fermented oil with a whitening effect and a preparation method and application thereof. The preparation method comprises the following steps: (1) inoculating a tricholoma matsutake endophytic fungus seed solution into a fermentation culture medium for fermentation culture to obtain a primary fermentation solution; and (2) mixing the primary fermentation liquor with vegetable oil and a phosphatidylcholine-based eutectic solvent, continuing fermentation culture, and centrifugally collecting an upper-layer oil phase to obtain the tricholoma matsutake endophytic fungus fermentation oil. The tricholoma matsutake endophytic fungus fermentation oil prepared by the invention has high-level transdermal capabilities of oil-soluble functional components and water-soluble functional components, and has an excellent whitening effect.

The invention discloses a method for producing phosphatidylserine through enzyme catalysis, and belongs to the technical field of biology, and the method specifically comprises the following steps: in a reaction system of a [BMIM] [BF4] aqueous solution, taking natural phospholipid containing phosphatidylcholine and cephalin as a raw material, adding L-serine, calcium salt and phospholipase D, and carrying out a trans-acylation reaction. By introducing cephalin (4%-10%, preferably 6%-8%) and sphingomyelin (5%-10%) in a specific proportion and combining with a [BMIM] [BF4] ionic liquid system, high efficiency and high purity (the purity gt, 79.6% and the yield gt, 76.4%) of PS production are realized, and the problem that the yield and the purity cannot be considered at the same time in the traditional process is thoroughly solved.

This invention discloses a method for preparing a highly stable polydopamine-lipid bilayer membrane. On one side of a porous Teflon membrane scaffold, dopamine and polyethyleneimine self-polymerize at the interface of an alkaline buffer and air to form a porous polymer monolayer membrane. Simultaneously, on the other side, diaphytylphosphatidylcholine self-assembles at the solution / air interface to form a lipid monolayer membrane. A solution-lifting method is used to spontaneously fuse the Teflon pores to form a polymer / lipid bilayer composite membrane. This asymmetric composite membrane exhibits excellent mechanical strength, stability, and high sealing performance. Membrane proteins, protein channels, or protein nanopores can spontaneously and rapidly insert into the composite membrane to form channels, enabling single-molecule pA-level current or optical measurements. Furthermore, protein biomolecules such as enzymes, antigens, or antibodies can be sandwiched and sealed between the composite membranes, utilizing spatial effects to achieve label-free single-molecule detection.

This invention relates to the field of cigar processing technology, and more particularly to a method for grouping and processing cigar tobacco scraps. It includes the following steps: Grouping: The scraps are grouped according to whether they contain tobacco stems, their moisture content, and their origin; the scraps with medium moisture content and containing tobacco stems undergo vacuum rehydration treatment; the scraps with low moisture content and containing tobacco stems undergo vacuum rehydration and hot air moistening treatment sequentially; simultaneously, the scraps with medium and low moisture content are further divided into thin and thick groups, the thin group undergoes water rehydration and phosphatidylcholine aqueous solution rehydration, and the thick group undergoes sodium chloride aqueous solution rehydration and water rehydration; then all the scraps containing tobacco stems are processed together and then combined with the stemless scraps that have undergone hot air moistening treatment for subsequent processing. This application, through refined pretreatment after grouping cigar tobacco scraps according to whether they contain tobacco stems, their moisture content, and their origin, homogenizes the processing characteristics of different cigar tobacco scraps, thereby improving raw material utilization.

The invention relates to an alkaloid liposome, a preparation process thereof and an application of the alkaloid liposome in an external preparation, and belongs to the technical field of external preparations. The technical problem to be solved is to provide an alkaloid liposome technology which is higher in stability, safe and non-irritating. According to the scheme, the alkaloid liposome technology is characterized in that raw materials comprise alkaloid, hydrogenated lecithin, grease, polyhydric alcohols and water; the alkaloid is selected from at least three of piperine, tetrahydropiperine, tetrahydropalmatine, dihydrooat alkaloid D, caffeine and leonurine; the hydrogenated lecithin is selected from at least one of PHOSPHOLIPON80H or PhoslipTM HPC (Hydroxyethyl Propyl Phosphatidylcholine). The liposome is good in stability and low in irritation, and has the effects of promoting local tissue microcirculation, reducing choked flow rate, increasing local body temperature, enhancing tissue metabolism and improving local inflammation when being externally applied to skin.

The present application discloses PLPC-DB, an ultrapurified phospholipoproteomic composition derived from the supernatant of peripheral blood mononuclear cells (PBMCs). It comprises essential phospholipids, bioactive proteins, regulatory peptides, and intercellular signaling factors. The composition exceeds 99% purity through a multi-stage purification process combining high-speed centrifugation and selective ultrafiltration (1-50 kDa), followed by lyophilization. This process ensures structural stability for ≥24 months and inter-batch variability <2%. PLPC-DB supports reproducibility and molecular integrity in research and diagnostic settings. Key components include phosphatidylcholine and phosphatidylserine for membrane stabilization and intracellular signaling; structural and regulatory lipids for membrane biogenesis and immune-relevant components involved in antigen presentation, cytokine modulation, and membrane-associated signaling. These may include structurally defined molecules compatible with immunological evaluation in experimental and non-therapeutic settings. The composition is formulated for bioaccessible delivery via sublingual, endonasal, transdermal, and injectable routes, and is intended exclusively for non-therapeutic use in experimental and translational models.

The invention relates to an extracellular vesicle enrichment material as well as preparation and application thereof. According to the enrichment material, SPP serves as a monomer, AIBN serves as an initiator, and a hydrophilic amphoteric high-molecular polymer is prepared through a precipitation polymerization reaction. The efficient capture of the extracellular vesicles is realized by utilizing the coordination effect of the prepared amphoteric high-molecular polymer and phosphatidylcholine on the surfaces of the extracellular vesicles. Meanwhile, the material is subjected to phase change centrifugation in an organic phase with a certain concentration to form a precipitate, and one-step efficient enrichment of the extracellular vesicles is realized by utilizing the characteristic. The material contains repeated amphoteric group units, so that the material has strong affinity to extracellular vesicles, and meanwhile, the strong hydrophilicity of the material can also avoid non-specific adsorption of impure proteins, so that the enrichment purity of the extracellular vesicles is remarkably improved. In addition, the material has the advantages of being easy to prepare, simple and convenient to operate, efficient in enrichment and the like, and has important significance on separation and purification and downstream analysis of extracellular vesicles in body fluid.

The invention relates to the technical field of sterilization and antifogging, in particular to a microsphere-based antibacterial and antifogging additive, a preparation method thereof, a multifunctional coating and application. The microsphere-based antibacterial and antifogging additive is a liposome vesicle microsphere, and the liposome vesicle microsphere is prepared from an oil-phase raw material and a water-phase raw material; the oil phase raw material comprises one or more of distearoyl phosphatidylcholine, hydrogenated lecithin and cholesterol; the water-phase raw material is a mixture of polyhexamethylene biguanide, polyethyleneimine and benzalkonium chloride; in the water phase raw material, the content of the polyhexamethylene biguanide is 25wt%-45wt%. By optimizing the formula of the microspheres, the efficiency and effect of killing nucleic acid can be remarkably improved, and the fog-resistant and anti-fog effects are achieved.

The invention relates to the technical field of disinfectants, in particular to environment-control double-layer composite microbe removal microspheres and a preparation method thereof. The environment-control double-layer composite microbe removal microsphere comprises an inner core and a shell wrapping the inner core, the inner core is prepared from water, nano disinfection microspheres and a stabilizer, and the shell is prepared from an organic solvent and polylactic acid; the nano disinfection microspheres are prepared from an oil-phase raw material and a water-phase raw material, the oil phase raw materials comprise hydrogenated lecithin, distearoyl phosphatidylcholine and cholesterol; the water-phase raw materials comprise polyethyleneimine, polyhexamethylene biguanide and benzalkonium chloride. According to the environment-control double-layer composite microbe removal microsphere, through unique structural design, the nanometer disinfection microsphere in the inner core is prepared from specific oil-phase and water-phase raw materials, various pathogens can be effectively killed, and the polylactic acid structure of the shell provides good stability and slow release performance for the microsphere, so that the environment-control double-layer composite microbe removal microsphere has a good application prospect. And the duration of the disinfection effect is prolonged.