The invention provides a traditional Chinese medicine suppository which is specially used for treating gynaecological vaginitis and a preparation method thereof, according to the pathogenic mechanism and therapeutic principle of the gynaecological vaginitis and the theory of modern traditional Chinese medicine. In the suppository, radices sophorae flavescentis, herba violae, fructus citri sarcodactyli, radix gentianaes and szechuan lovage rhizome, which can clear heat, dry dampness, dispel wind, arrest itching, antisepticise, subdue inflammation and disinfest, are taken as monarch drugs; fructus cnidii, safflower, motherwort herbs, honeysuckle and hydrargyri subchloridum, which can dispel wind, disinfest, treat pruritus vulvae and leucorrhea for cold wetness, are taken as ministerial drugs; fructus kochiae, catechu, matricaria indica and garden burnet root, which can strengthen the curative effects of the monarch drugs and the ministerial drugs, are taken as excipients; and mint, skullcap, lotus plumule, divaricate saposhnikovia root, borneol and seabuckthorn seed oil, which can clear away the heart fire, nourish yin, promote the production of body fluid, and eliminate wetness-evil with fragrant flavor, are taken as adjuvant drugs. The combination of the monarch drugs, ministerial drugs, excipients and the adjuvant drugs can display the inwardness of the drugs and combine the effects of the drugs, thereby changing the composition of cell membrane lipids and killing the epiphyte. Therefore, the suppository has good curative effects on the vulvitis, colpomycosis and trichomonas vaginitis with short course of treatment and high cure rate, and can realize the radical cure of the diseases.