13 results about "Pectobacterium" patented technology

The present invention discloses three soft rot pathogen-specific detection genes and their application in detecting taro soft rot. The present invention has obtained specific gene fragments for detecting Dickea fangzhongdai, Pectobacterium aroidearum and Pectobacterium colocasium, and their nucleotide sequences are shown as SEQ ID NO.1, SEQ ID NO.2 and SEQ ID NO.3, respectively. Based on this, a set of primer groups and detection products that can specifically detect the three pathogens have been developed, which can detect the three pathogens separately or simultaneously. The present invention uses molecular detection technology for the first time to detect and identify the above three pathogens of taro soft rot. The detection method of the present invention is fast, simple, specific, and sensitive. It can achieve specific detection in the early stage of invasion, and has important significance and application value for predicting the condition of related diseases, especially taro soft rot, and taking timely measures to prevent and control it.

This invention belongs to the field of biotechnology. To improve the effectiveness of biological control of plant diseases, this invention discloses *Bacillus velezensis* ZF516 and its application in controlling various soil-borne diseases. The *Bacillus velezensis* strain number is ZF516, and its registration number at the China General Microbiological Culture Collection Center is CGMCC No. 30136. This *Bacillus velezensis* strain can prevent and / or treat tomato bacterial canker, tomato bacterial wilt, or cowpea root rot, or can be used to prepare products for the prevention and / or treatment of these diseases. This strain also exhibits good inhibitory effects against *Fusarium oxysporum*, *Diplostomum spp.*, *Rhizoctonia solani*, *Fusarium solanum*, *Corynebacterium multiflorum*, *Botrytis cinerea*, *Agrobacterium tumefaciens*, *Pectinobacter brasiliensis*, *Ralstonia solanacearum*, *Streptomyces spp.*, etc.

The invention discloses a salt-tolerant nitrogen-fixing Klebsiella pasteurii and application thereof, and relates to the technical field of microorganisms, and the Klebsiella pasteurii S8812 provided by the invention is a salt-tolerant combined nitrogen-fixing bacterial strain which is screened from corn roots and has outstanding comprehensive performance; the strain is preserved in the China General Microbiological Culture Collection Center (CGMCC) on March 21, 2025, and the preservation number of the strain is CGMCC No: 33923. The strain is high in nitrogen fixation activity, strong in salt resistance and capable of tolerating a culture medium environment with the NaCl concentration as high as 7%. Meanwhile, the strain also has the functions of dissolving organic phosphorus, dissolving potassium, producing amylase, producing ammonia, producing siderophore, producing auxin and the like. Greenhouse pot experiments show that the strain has a growth promoting effect on various crops such as wheat and corn, and the plant height, stem length, fresh weight and the like of plants can be remarkably improved. In addition, the strain has a good inhibition effect on rhizoctonia solani, pectobacterium and xanthomonas oryzae.

The invention relates to the technical field of medicinal botanical bactericides, and particularly discloses application of kushenol F in preparation of broad-spectrum antibacterial drugs. It is found that the kushenol F can be applied to inhibition of growth of various bacteria such as pectobacterium, pseudomonas aeruginosa, bacillus cereus and ralstonia solanacearum. The kushenol F shows a remarkable inhibition effect on Ralstonia solanacearum, can obviously inhibit mycelial growth, and is suitable for preventing and treating agricultural diseases such as bacterial wilt and the like. Compared with traditional chemical synthetic antibiotics, the sophora alcohol F has the advantages of being low in toxicity, safe to use, high in antibacterial activity, wide in application range and the like, and a new choice is provided for bacterial disease control in the fields of agricultural production and medicine.

The invention discloses paenibacillus polymyxa JX-1 and application of the paenibacillus polymyxa JX-1. The bacillus polymyxa strain JX-1 is preserved in the China General Microbiological Culture Collection Center on March 10, 2025, the preservation date is CGMCC (China General Microbiological Culture Collection Center), and the preservation number is CGMCC NO.33781. The bacillus polymyxa strain JX-1 is named as Paenibacillus polymyxa strain JX-1 and is named as Paenibacillus polymyxa strain JX-1. The paenibacillus polymyxa JX-1 disclosed by the invention can be used for remarkably preventing and treating the influence of plasmodiophora brassicae infection on hosts and promoting the growth of plant root systems. The paenibacillus polymyxa JX-1 disclosed by the invention can also be used for remarkably preventing and treating the occurrence of mulberry fertilizer ginkgo biloba disease in field experiments. Various extracellular enzymes and biological membranes can be generated, and thalli and culture supernate can antagonize various plant pathogens including fusarium, sclerotinia sclerotiorum, pectobacterium, ralstonia solanacearum and oryza sativa.

The utility model provides a device for extracting lysate of obligate bacteriophage of soil ralstonia solanacearum, and relates to the technical field of enrichment and separation of bacteriophage, the device comprises a centrifugal tube and an enrichment tube, the centrifugal tube is provided with a sample inlet and a sampling port, the inside of the centrifugal tube is provided with an accommodating cavity communicated with the sample inlet and the sampling port, and the enrichment tube is arranged in the accommodating cavity. The enriching pipe is detachably mounted in the accommodating cavity, the enriching pipe is provided with a sample receiving port and a filter port, the sample receiving port is communicated with the filter port, the sample receiving port is arranged towards the sample inlet, and a filter screen is arranged at the filter port. According to the utility model, enrichment and separation of bacteriophages can be completed in the same equipment.

The present application belongs to the technical field of microorganisms, and particularly relates to a bacillus pumilus, a microbial agent, a method and application. The present application provides a bacillus pumilus G15 with a preservation number of CGMCC No.32839. The bacillus pumilus G15 of the present application has a significant inhibitory effect on a variety of pectobacterium FN20111. The present application further provides a microbial agent. Potting prevention effect determination shows that the bacillus pumilus G15 has a high prevention effect on the variety of pectobacterium FN20111.

The application belongs to the technical field of plant protection, and particularly relates to application of pyrimidine nucleoside compounds in prevention and treatment of plant bacterial diseases. The application provides application of pyrimidine nucleoside compounds and their pesticide-acceptable salts in preparation of medicines for preventing and / or treating plant bacterial diseases. Bacteriostatic activity tests show that the compounds exhibit excellent inhibitory activity on multiple plant pathogenic bacteria such as rice bacterial leaf blight, rice bacterial leaf streak, tobacco bacterial wilt, soft rot pectobacterium, wild mustard xanthomonas campestris and sweet potato bacterial stem rot, and exhibit broad-spectrum anti-plant pathogenic bacterial ability. The application widens the application range of pyrimidine nucleoside compounds in agriculture, enriches the types of medicines for preventing and treating plant bacterial diseases, and provides a novel and effective solution for plant bacterial diseases.

The invention discloses bacillus velezensis QJXW44 and an application of the bacillus velezensis QJXW44 in amorphophallus konjac soft rot. The bacillus velezensis QJXW44 is preserved in China General Microbiological Culture Collection Center on May 10, 2024, and the preservation number of the bacillus velezensis QJXW44 is CGMCC No.30596. The invention further discloses an application of the bacillus velezensis QJXW44 in amorphophallus konjac soft rot. The bacillus velezensis QJXW44 provided by the invention has an antagonistic effect on various plant pathogenic fungi including Erwinia carotovora subsp. Carotovora, pectobacterium carotovora and botryosphaeria dothidea, has a remarkable antagonistic effect on the soft rot of the konjak, and also can induce the disease resistance of the konjak; the microbial community structure at the rhizosphere of the konjak is improved, the occurrence and spreading of the soft rot of the konjak can be effectively inhibited through a biological control mode, and the use of chemical pesticides is reduced.

The invention discloses a Pth-St1 antibacterial derived peptide and application thereof, and belongs to the field of application of peptide antibiotics. The amino acid residue sequence of the antibacterial derived peptide (Design1867) is as follows: RKLVRQLHRFKGKLVRKLH. The invention further discloses a preparation method of the antibacterial derived peptide (Design1867). The derivative peptide provided by the invention has a stronger bactericidal effect, and the minimum bactericidal concentrations of the derivative peptide to escherichia coli and pectobacterium soft rot reach 250 mu g / mL and 62.5 mu g / mL respectively; meanwhile, the safety of the Design1867 polypeptide is also proved, so that the Design1867 polypeptide is expected to become a novel prevention and control product and means for pectobacterium soft rot.

The application discloses Paenibacillus polymyxa JX-1 and application thereof. The Paenibacillus polymyxa JX-1 is preserved in the China General Microbiological Culture Collection Center on March 10, 2025, and the preservation number is CGMCC NO.33781. The Paenibacillus polymyxa JX-1 can significantly prevent and treat the influence of the infection of gnomoniella on a host, and promote the growth of a plant root system. The Paenibacillus polymyxa JX-1 can also significantly prevent and treat the occurrence of a large-sized white fruit disease of mulberry in a field experiment. The Paenibacillus polymyxa JX-1 can produce various extracellular enzymes and biological membranes, and the bacterial body itself and culture supernatant can antagonize various plant pathogenic bacteria including fusarium, sclerotinia, pectobacterium, pseudomonas, and cuphelia mori.

The invention belongs to the technical field of microorganisms, and particularly relates to bacillus velezensis, bacillus pumilus, a biocontrol agent, a method and application. The invention provides a bacillus velezensis HzR6-0, and the preservation number of the bacillus velezensis HzR6-0 is CGMCC (China General Microbiological Culture Collection Center) The bacillus velezensis HzR6-0 disclosed by the invention has a remarkable inhibition effect on pectobacterium parvovium FN20211. The invention also provides a biocontrol agent, and the result of the embodiment shows that the bacillus velezensis HzR6-0 and bacillus pumilus G15 compounded fermentation liquor and 4% kasugamycin (aqueous solution) are applied to the field for prevention and treatment, the prevention and treatment effect on the potato aerial stem rot is the best, and the prevention and treatment effect is 40.66%.

The invention discloses a single-tube one-step nucleic acid detection method of pectobacterium brazilianum and a kit used by the same. The invention relates to the field of vegetable disease detection, and provides a nucleic acid detection reagent for detecting pectobacterium brazilianum, which comprises a reagent A and a reagent B, the reagent A is a reagent for performing RPA amplification on target nucleic acid of pectobacterium brazilianum; the reagent B is a reagent for carrying out CRISPR / Cas detection on the target nucleic acid; the reagent B contains polyvinyl alcohol. According to the invention, prefabrication and room temperature storage of a reaction system are realized, on-site preparation is not needed, operation is simple, reaction only needs one-step pipetting, aerosol pollution is avoided, cost is low, and detection of pectobacterium brazilianum can be completed without large-scale instruments and equipment and temperature control equipment with relatively high energy consumption. The invention provides an efficient, reliable and economical field detection solution for diagnosing vegetable diseases caused by pectobacterium brazilianum.