1393 results about "Bacillus cereus" patented technology

The invention relates to a group of alive microbial preparations for preparing compound microbial fertilizer and the preparation method thereof. The alive microbial preparations include a microbial inoculant and an organic material degradation agent. The microbial inoculant includes Bacillus megaterium, Bacillus licheniformis, Bacillus mucilaginosus, Bacillus laterosporus, Azotobocter chroococcum, etc. The organic material degradation agent includes Bacillus megaterium, Bacillus licheniformis, Bacillus subtilis, Bacillus circulans, Saccharomyces cerevisiae, Candida lipolytica, Bacillus pumilus, Bacillus cereus, Streptomyces griseus, Pseudomonas alcaligenes, Pseudomonas cepacia, Streptococcus salivarius subsp. Thermophilus, Geobacillus stearothermophilus, Streptomyces Thermophilus, Geotrichum candidum and Aspergillus oryzae. The preparation method comprises the following steps: carrying out submerged fermentation, directly mixing the bacterial liquids and stirring to obtain a liquid preparation; or mixing with an absorbent to obtain a solid preparation. The alive microbial preparations have the advantages of improving soil fertility, promoting plant growth, enhancing anti-disease, anti-insect and anti-drought capacities of plants, improving quality of agricultural products and so on.

The present invention demonstrated the potential use of Lactobacillus johnsonii D115 as a probiotic, as a prophylactic agent or as a surface treatment of materials against human and animal pathogens such as Brachyspira pilosicoli, Brachyspira hyodysenteriae, Shigella sonnei, Vibrio cholera, Vibrio parahaemolyticus, Campylobacter jejuni, Streptococcus pneumoniae, Enterococcus faecalis, Enterococcus faecium, Clostridium perfringens, Yersinia enterocolitica, Escherichia coli, Klebbsiella pneumoniae, Staphylococcus aureus, Salmonella spp., Bacillus cereus, Aspergillus niger and Fusarium chlamydosporum. The proteineous antimicrobial compound was partially characterized and found to be heat tolerant up to 121° C. for 15 min, and acid tolerant up to pH1 for 30 min at 40° C. The compound is also stable to enzymatic digestion, being able to retain more than 60% antimicrobial activity when treated with pepsin and trypsin.

The present invention relates to microbial fodder additive, and a kind of microbial fodder additive comprising beneficial microbes and their metabolites and its preparation process and use. The microbial fodder additive is the mixture of fermented liquid or dry powder obtained with Bacillus subtilis, Bacillus cereus, lactobacillus and Saccharomyices cerevisiae and through high density deep layer liquid fermentation. It may be prepared into different specific liquid or powder preparations. The microbial fodder additive has high live bacillus content, long product preserving period and less foreign bacteria contamination, and has the functions of raise fodder utilization, promote animal's growth, increase animal product yield and quality, etc.

A low-temp nitrating bacteria preparation for removing NH3-N and COD from the seawater used in marine farm is prepared from Nitrobacter hamburgensis (AS 1.2729), Nitrobacter winogradskyi (AS 1.2730), Nitrobacter vulgaris (AS 1.2775), Bacillus cereus (AS 1.230), Bacillus licheniformis (AS 1.518) and saccharomyces cerevisiae (AS 2.412) through culturing, and proportional mixing.

The invention discloses a denitrifying strain with nitrifying function, a strain-containing water body improver of multiple active microorganisms and a preparation method of the water body improver, belonging to the technical fields of aquaculture and water body improvement. The strain disclosed by the invention is conserved in the China center for type culture collection (CCTCC), the CCTCC NO isM2011443; and the water body improver of the active microorganisms which is based on the strain comprises multiple strains such as Bacillus subtilis, Bacillus licheniformis, Bacillus pumilus, Bacillus cereus, Bacillus megaterium, Rhodopeudomonas palustris, Arthrobacter globiformis, Rhodotorula rubra and Lactobacillus plantarum. The strain is used to perform liquid fermentation and obtain fermentation liquor of which number of live bacteria is 0.5-3*10<10>cfu/mL, bacterial powder of which the number of live bacteria is 0.2-2*10<12>cfu/mL is further prepared; and the bacterial powder is used along with a diluent to prepare a liquid or powdery water body improver, wherein the effective live bacteria of the water body improver is 0.1-3*10<10>cfu/mL(or cfu/g). The water body improver has the function of reducing the contents of ammonia nitrogen, nitrites and nitrates in the culture water body; and the water body improver is suitable for different culture water bodies and has the function of improving the water quality.

The invention discloses a compound probiotics preparation for biological organic fertilizers and a preparation method thereof. The preparation method comprises the following steps: performing activated culture of 14 kinds of probiotics, including Pseudomona putida, Streptomyces violovariabilis, Rhizobium sp., Azotobacter chroococcum, Bacillus subtilis, Bacillus amyloliquefaciens, Bacillus cereus, Bacillus subtilis, Bacillus weihenstephanensis, Bacillus mucilaginosus, Bacillus thuringiensis, Saccharomyces rouxii, Streptomyces griseus var. ferrugineus, and Streptomyces microflavus; performing mixed inoculation at a proportion of 1% to 5% to a 500 mL liquid seed culture medium; then performing amplification culture; and finally delivering the strains to a 500 mL fermentor and fermenting for 3 to 5 days under such conditions that the temperature ranges from 28 DEG C to 30 DEG C and the rotational speed is 200 rpm, to obtain a liquid-state compound probiotics preparation. The compound probiotics preparation for biological organic fertilizers, provided by the invention, plays important roles of taking advantage of microbial ecology, improving soil, promoting plant growth, improving the yield and disease resistance of crops and reducing the consumption of fertilizers.

The invention relates to a compound microbial bacterial preparation for degrading crop straw and a preparation method and application of the compound microbial bacterial preparation. The compound microbial bacterial preparation is prepared from bacillus subtilis, bacillus cereus, aspergillus niger, aspergillus flavus, trichoderma reesei, trichoderma longibrachiatum, sporotrichum thermophile and phanerochaete chrysosporium through solid fermentation. The compound microbial bacterial preparation can shorten the decomposition time and improve the decomposition efficiency of the crop straw. Microorganisms generate various effective metabolic products with nitrogen fixation and phosphate solubilizing functions and a high disease and pest resistance effect in the fermentation process, so that the compound microbial bacterial preparation is favorable for improving the soil structure and the yield and the quality of crops.

The invention discloses a production method of L-lactic acid and the special condensate bacillus CASH CGMCC No. 2184. L-lactic acid can be gotten by cultivating the special condensate bacillus CASH CGMCC No. 2184. Per litter of fermentation culture medium of the condensate bacillus comprises at least one of the following five nitrogen sources: yeast powder 5 to 15g, peptone 5 to 15g, soybean peptide 5 to 15g, soybean peptone 5 to 15g, and cottonseed protein 10 to 20g; in addition, the fermentation culture medium comprises glucose 80 to 170g (the content of initial glucose), calcium carbonate 50 to 100g and water; the pH of the fermentation culture medium is 5.5 to 7. With glucose as the substrate, under the condition of 45 to 60DEG C and with 98 to 99% of sugar acid conversion, the condensate bacillus CASH CGMCC No. 2184 efficiently ferments to produce L-lactic acid with optical purity of 99%; the concentration of the L-lactic acid maximum can attain 190 g/l and the volume production efficiency maximum can attain 5.7 g/l/h.

The invention relates to an organic materials zymolysis complex bacillus agent in the field of zymolysis bacillus agent technology. It comprises: low temperature bacteria, Bacillus cereus, gliotoxin, pseudomonas, slim streptomycete, high temperature bacteria and high temperature actinomyces with the weight ration 4:2:1:1:3:2:2. The low temperature bacterium is enzyme yeast with the heat resisting temperature below 20 deg. The high temperature bacterium is enzyme yeast with the heat resisting temperature below 70 deg. The heat resisting temperature below 70 deg of the high temperature actinomyces bellows 70 deg.

The present invention discloses a compound microbial feed additive. It is a compound microbial feed additive obtain by adding 7 normal microbial pools into solid culture medium and making solid fermentation, drying and pulverizing to obtani powered material. Said 7 liquid micriobial pools are bacillus cereus, peptococcus, lactic acid bacteria, lactobacillus bifidus, yeast, photosynthetic bacteria and actionmycete. In said invented feed additive its viable count is greater than or equal to 180 handred million/gand total number of bacteria is greater than or equal to 240 handred milling/g. Said additive can be aded into animal feed, and can reduce fed-meat ratio and can raise disease resistance of animal.

The invention relates to a compound microbial fertilizer and a preparation method thereof. The compound microbial fertilizer is prepared by mixing the following raw materials: yeast fermentation powder residues, an organic fertilizer, urea, K2SO4, NH4H2PO4, clay and a compound microbial agent; and the compound microbial agent is formed by fermentation of bacillus mucilaginosus, bacillus megaterium, bacillus cereus, bacillus thuringiensis, azotobacter chroococcum, pichia farinose and streptomyces microflavus. The compound microbial fertilizer disclosed by the invention has a variety of microbes with the effects of nitrogen fixation, phosphorus dissolution and potassium dissolution, can increase the nitrogen content in soil, improves the utilization rate of the chemical fertilizer, and is favorable for improving the structure of the soil and improving the yield and quality of crops; and simultaneously, the microbes in the fertilizer can produce a variety of metabolites, and the metabolites can effectively resist diseases and insect pests.

A method of detection is provided that permits differentiation of each of Bacillus cereus, Bacillus thuringiensis, and Bacillus anthracis from other microorganisms, using oligonucleotide primers for amplification of the target nucleotide sequences characteristic to Bacillus cereus, Bacillus thuringiensis, and Bacillus anthracis, consisting of the oligonucleotide (A) having a nucleotide sequence obtained from SEQ ID NO:1 and containing at least one site that can amplify a nucleotide sequence characteristic to Bacillus cereus, the oligonucleotide (B) having a nucleotide sequence obtained from SEQ ID NO:3 and containing at least one site that can amplify a nucleotide sequence characteristic to Bacillus thuringiensis, and the oligonucleotide (C) having a nucleotide sequence obtained from SEQ ID NO:5 and containing at least one site that can amplify a nucleotide sequence characteristic to Bacillus anthracis. Also provided are a method of detection of Bacillus cereus, Bacillus thuringiensis, and Bacillus anthracis by polymerase chain reaction (PCR) using a primer specific to the DNA gyrase sub-unit B (gyrB) gene and a method of detection of Bacillus cereus, Bacillus thuringiensis, and Bacillus anthracis in a sample by differentiation on the genetic level.

The invention discloses a fourteen-food-borne pathogenic bacterium multiplex PCR detection primer set and a kit. The detection primer set is composed of primer pairs for detecting salmonella, Shigella, Vibrio parahemolyticus, campylobacter jejuni, campylobacter coli, staphylococcus aureus, bacillus cereus, Listeria monocytogenes, yersinia enterocolitica, enterobacter sakazakii, Escherichia coli, vibrio cholerae, Escherichia coli O157, aeromonas hydrophila and internal positive control. A multiplex PCR detection method based on an ordinary PCR platform is built, multiplex PCR reactions are carried out on genomic DNA, extracted from a sample to be tested, of bacteria in the same reaction system through the primer pairs acquired through analysis and design, and whether the food-borne pathogenic bacteria are contained in the sample or not is judged through the electrophoretic analysis of reaction products.

A microecological water quality conditioning agent applicable to restoring of sea cucumber culture water body and a method for preparing the microecological water quality conditioning agent belong to the technical field of aquaculture and microorganisms. By aiming at physiological habits and culturing characteristics of sea cucumbers and applying modern biological technique, the microecological water quality conditioning agent is made of bacillus flexus CGMCC (China general microbiological culture collection center) No.5115, bacillus subtilis CGMCC No.5116 and bacillus cereus CGMCC No.5117, which are separated and sieved from a sea cucumber culturing environment and prepared by steps: using corn starch and soybean cake powder as main culturing materials, fermentating for 24-48h at 25-40 DEG C, and using zeolite and wheat bran as adsorption carriers for processing to obtain the microecological water quality conditioning agent. The microecological water quality conditioning agent is less susceptible to environment conditions, has remarkable purifying effect on sea cucumber culturing water body, and is capable of greatly reducing content of harmful substances such as ammonia nitrogen, nitrite, hydrogen sulfide and the like, deodorizing and effectively restraining pathogenic bacteria so as to prevent sea cucumber diseases and improve yield and quality of cultured sea cucumbers.

The invention relates to a complex microorganism fungicide for treating animal manure and a preparation method and an application thereof. The adopted technical scheme is that the complex microorganism fungicide consists of saccharomyces cerevisiae, powdery pichia, bacillus subtilis, bacillus cereus, bacillus megaterium and lactobacillus plantarum. Microbial organic fertilizer can be prepared by treating the animal manure with the complex microorganism fungicide. The complex microorganism fungicide for treating the animal manure prepared by the invention has the advantages of easy obtainment of raw materials and simple manufacturing method. Preparing the microbial organic fertilizer by mixing the complex microorganism fungicide for treating the animal manure with the animal manure, organic materials and cornmeal not only can treat the animal manure, but also effectively utilizes rural wastes; and the prepared microbial organic fertilizer can not pollute crops and cultivated land because no chemical substance exists in the prepared microbial organic fertilizer.

The improved process of treating oil field effluent for reinjection features aerobic biochemical treatment and subsequent filtering. The microbes for the aerobic biochemical treatment include Bacillus cereus, Corynebacterium, Bacillus subtilis and Pseudomonas alcaligenes, and the inflow water has pH value controlled in 6-9. The process can reach oil field water injection standard, including oil not more than 2 mg/l, SS not more than 1 mg/l and grain size mid value not greater than 1 micron. The present invention has the advantages of simple technological process, low cost, stable treating effect, no secondary pollution, etc., and is especially suitable for use in oil field.

The invention discloses a composite bacillus preparation applied to improving the aquatic environment of mariculture, and a making method thereof. The preparation is made by mixing Bacillus subtilis, Bacillus licheniformis, Bacillus cereus and Bafillus natto according to certain ratio, and under room temperature, the active microorganism is in resting state and the preparation is stable and easy to be preserved. After the composite bacillus preparation is put into mariculture water, the dormant bacteria can be back to normal quickly, degrade residual bait, dejection, fertilizer, biological residual bodies and other organic contaminant fast, reduce the content of ammonia nitrogen, nitrite and COD in the culture water and reproduce to be predominant bacteria in the water quickly so as to further restrain the putrefying bacteria from reproduction, improve the environment of the culture water and purify the water. The preparation of the invention is safe, contains no pollution nuisance and residual, can obviously improve the environment of the culture water, and meanwhile, the immunity of aquatic livestock can be improved.

The invention provides a cadmium pollution bioremediation agent which is composed of the following ingredients in parts by weight: 10-20 parts of core rhzomorph, 10-20 parts of a nutritional ingredient, 2-6 parts of an enzyme activity substance and 54-78 parts of an rhzomorph carrier. The invention further provides an application of the cadmium pollution bioremediation agent. The cadmium pollution bioremediation agent is reasonable in microorganism specie collocation and high in comprehensive function. Microorganisms in the core rhzomorph can be planted and grow in soil rapidly to form strong bacterial colonies, and heavy metal ions, namely, cadmium Cd<+1> can be directly absorbed to microbial cell walls or into cells to be cured and passivated. Besides, bacillus cereus and beer yeast in the composition have high absorption and passivation function on heavy metal ions, namely, cadmium Cd<+1>, chromium Cr<+1> and lead Pb<+1>, due to the high comprehensive function, the mobility of the heavy metal ions, namely, cadmium Cd<+1>, is reduced, and the purpose of reducing absorption of crops on the heavy metal ions is achieved.

The bacterial colony number sample for verifying food microbiological capacity relates to quality control technology in microbiological detection. The sample is matrix with added Bacillus cereus as leading bacteria and background bacteria. The matrix is practical food, and the background bacteria include one or several kinds of serratia marcescens, Citrobacter freundii, enteroaerogen and escherichia coli. The sample of the present invention has homogeneous colony number content, high finishing rate, and high stability, and may be used in test capacity verification of domestic and international labs. In the same time, the capacity verifying sample may be used in verification of detection method, calibration of test instrument, quality control and check of test result and other aspects.

The invention relates to a high-efficiency hardening-resisting continuous-cropping-resisting soil-modifying biological agent. The biological agent is composed of: 20% of trichoderma, 20% of streptomyces microflavus, 30% of starch, 10% of grass peat, 8.15% of a siliceous fertilizer, 6% of humic acid, 5% of urea, 0.2% of biological strains (including 1/3 of bacillus subtilis, 1/3 of bacillus cereus, and 1/3 of bacillus licheniformis), 0.1% of vitamin C, 0.5% of compound sodium nitrophenolate, and 0.05% of DA-6. With the soil-modifying biological agent provided by the invention, defects of existing fertilizers are overcome, the pollution to the environment is reduced, soil is activated, soil hardening and continuous-cropping are overcome, and substantial influences are provided for healthy growth and bumper harvest of crops.

The invention discloses a compound enzyme preparation for ramie degumming, and a preparation method thereof. The method comprises the steps of taking bacillus cereus obtained through separation as an original strain, preparing high-enzyme-activity alkaline pectinase which contains glucanase and neutral protease through seed culture and liquid-submerged fermentation, and compounding an appropriate amount of xylanase and mannanase on the basis of the alkaline pectinase so as to prepare the compound enzyme preparation. The invention also discloses a ramie degumming process, and the compound enzyme preparation is applied to an enzyme refining step in the ramie degumming process. The process enables the alkaline pectinase and hemicellulase to play a role jointly by compounding a plurality of biological enzymes, so as to achieve the aim of degumming. The process can save acid-base raw materials, and has the advantages of mild treatment conditions, low cost, good fiber quality, little pollution on environment and the like.

The invention discloses a microbial preparation used for treating landfill leachate and a preparation method thereof; the preparation method of the microbial preparation is as follows: (1) a liquid culture medium I and a liquid culture medium II are prepared; (2) bacillus cereus is inoculated into the liquid culture medium I for culture and then inoculated into the liquid culture medium II for mass production, thereby obtaining bacterial suspension; Bacillus marisflavi is inoculated into the liquid culture medium I for culture and then inoculated into the liquid culture medium II for mass production, thereby obtaining the bacterial suspension; photosynthetic bacteria, nitrifying bacteria, denitrifying bacteria and yeast are respectively inoculated into the liquid culture medium II to be cultured into the bacterial suspension after activation; and (3) the microbial preparation is prepared by mixing the various types of bacterial suspension according to proportion. The microbial preparation can improve the removal rate of organic matters which are difficult to decompose and ammonia nitrogen in the salt-containing landfill leachate. The microbial preparation still has very high biological activity when being added into organic waste water with high salt content, thereby improving the removal effect of COD and ammonia nitrogen in the leachate.

The invention relates to a microbial preparation for shortening a tobacco fermentation period and application of the microbial preparation, and relates to the technical field of microorganism and tobacco processing. The microbial preparation comprises bacillus cereus and bacillus amyloliquefaciens from tobaccos, and the ratio of the live bacillus cereus to the live bacillus amyloliquefaciens is 10-200:1-50. The powdery microbial preparation is obtained by means of separation, culture and preparation and is dissolved into sterile water according to the proportion of 0.5-5%, formed water aqua is uniformly sprayed on tobaccos according to the proportion of 3-10%, and the tobaccos are artificially fermented for 10-40 days at the temperature ranging from 25 DEG C to 50 DEG C and the humidity ranging from 65%RH to 80%RH. Tests show that cellulose, protein, pectin and the like in the tobaccos can be degraded effectively, and the tobacco fermenting period is shortened to 10-40 days. Artificially fermented tobaccos are not obviously different from naturally alcoholized tobaccos in terms of quality, the tobacco fermenting period is shortened, and the quality of the tobaccos can be improved.

The invention discloses bacillus cereus with heavy metal passivating and plant growth promoting functions and application of the bacillus cereus, and belongs to the technical field of microbial strains. The name of the bacillus cereus is bacillus cereus B19, and is preserved in the China General Microbiological Culture Collection Management Committee Centre of Institute of Microbiology, Chinese Academy of Sciences, 3#, No.1 Yard, West Beicheng Road, Chaoyang District, Beijing, on April 27th, 2016; the preservation number is CGMCC NO. 12405. The bacillus cereus has the functions of passivating heavy metals and promoting plant growth, is suitable for decomposing difficultly soluble phosphorus, and is used for producing auxin, resisting salt, resisting the heavy metals, promoting plant seed germination, increasing effective phosphorus and microbial carbon and nitrogen of soil, and reducing available heavy metals in the soil.