172 results about "Bacillus cereus" patented technology

The invention discloses a bacterium capable of resisting serious combined pollution of arsenic and cadmium. The bacillus cereus strain is identified as bacillus cereus, is named as bacillus cereus L1. GLUT, and is preserved in the China General Microbiological Culture Collection Center on April 10, 2024, the address is No.3, Yard 1, Beichen West Road, Chaoyang District, Beijing, and the preservation number is CGMCC NO.30292. The bacillus cereus strain is named as bacillus cereus L1. GLUT. The bacillus cereus L1. GLUT can resist 200 mg / L of As and 100 mg / L of Cd at the same time, can be prepared into a microbial agent to be combined with a hyperaccumulator, and has considerable application value in the aspect of remediation of arsenic and cadmium combined contaminated soil.

The invention discloses a method for inhibiting production of vomitoxin from bacillus cereus, which is characterized in that isoleucine is added into a substance needing to inhibit production of vomitoxin from bacillus cereus, preferably 5-20mM of isoleucine is added. According to the present invention, the content of the toxin is detected through the liquid chromatography-mass spectrometry, and the cytotoxicity experiment verification results show that the isoleucine can significantly inhibit the synthesis of the vomitoxin of the bacillus cereus in the culture medium and the matrix, and can effectively reduce the toxic effect of the toxin on HeLa cells; the invention provides a safe and efficient biological control means which is suitable for various food systems such as starch food, dairy products, meat products and the like. Isoleucine can be used as a novel non-toxic and harmless food additive and is low in cost; the use method is simple, the output of vomitoxin can be reduced by directly adding a trace amount of isoleucine, and the food flavor is not affected.

The invention belongs to the technical field of microorganisms, and particularly relates to bacillus cereus as well as a culture and application thereof. The preservation number of the bacillus cereus AK-LY1 is CCTCC (China Center For Type Culture Collection) NO: M 2025756. The bacillus cereus AK-LY1 disclosed by the invention is excellent in trypsin resistance, good in cholate resistance, acid resistance and high temperature resistance, good in inhibition performance on antibiotics such as strong penicillin and chloramphenicol, and relatively good in adhesion capacity in intestinal tracts, and provides a relatively good microecological preparation reference basis for expanding a bacillus cereus resource library and applying the bacillus cereus.

The invention belongs to the technical field of environmental engineering, and discloses a microbial agent for degrading malathion as well as a preparation method and application of the microbial agent. The microbial agent comprises the following raw materials: compound bacteria, a nutritional supplement and a biomass carrier material, wherein the compound bacteria comprise functional bacteria and synergistic bacteria, and the mass ratio of the functional bacteria to the synergistic bacteria is (1.2-1.8): 1; the functional bacteria comprise issatchenkia orientalis, nocardia, brevibacillus laterosporus and bacillus cereus; the synergistic bacteria comprise azospirillum brasilense, bacillus thuringiensis and lactobacillus buchneri. Functional bacteria with direct degradation capacity and synergistic bacteria with auxiliary functions are creatively compounded according to a specific proportion, meanwhile, the garlic straw powder is selected as a biomass carrier material, all the raw materials complement each other in function and have a synergistic effect, the degradation rate and thoroughness of malathion are remarkably improved, and the degradation efficiency of malathion is greatly improved. The defects of low degradation efficiency and poor environmental adaptability of a single strain are overcome.

The invention belongs to the technical field of microorganisms, and particularly discloses lactobacillus plantarum CX1-3-2 capable of producing gamma-aminobutyric acid and improving sleep and application of the lactobacillus plantarum CX1-3-2. The preservation number of the lactobacillus plantarum CX1-3-2 strain is CGMCC (China General Microbiological Culture Collection Center) No. 34340. The lactobacillus plantarum CX1-3-2 can achieve the effect of improving sleep by improving the levels of GABA and 5-HT in brain tissues and reducing the levels of glutamic acid Glu, dopamine DA and noradrenaline NE; the scavenging rate of DPPH free radicals of the strain reaches 96.4%, the scavenging rate of ABTS free radicals of the strain reaches 78.3%, and the strain has an excellent anti-oxidation effect; the diameters of inhibition zones of the strain on staphylococcus aureus, bacillus cereus, escherichia coli and pseudomonas fluorescens are all larger than 17 mm, and the strain has an excellent antibacterial effect.

This invention belongs to the field of microbial technology, specifically relating to a strain of *Lactobacillus plantarum* that produces antimicrobial peptides and its applications. The *Lactobacillus plantarum* is specifically *Lactobacillus plantarum* (…). Lactobacillus plants Lactobacillus plantarum AF-6, with accession number CGMCC No. 33716, is a product of this invention. It generates a variety of antimicrobial peptides with broad-spectrum antibacterial activity, inhibiting spoilage bacteria such as Escherichia coli, Bacillus subtilis, Salmonella, Micrococcus luteus, Pseudomonas aeruginosa, Bacillus cereus, and Staphylococcus aureus. This provides a more reliable and effective option for food safety preservation.

The invention belongs to the technical field of microbial fermentation, and relates to bacillus cereus capable of biologically converting limonene, the bacillus cereus is preserved in the China Center for Culture Collection, the classification name is Bacillus cereus GJ-02, the preservation number is CCTCC M 20251438, and the preservation date is June 23, 2025; according to the invention, the bacillus cereus strain capable of biologically converting limonene and having efficient conversion capability is separated and screened, a new strain resource is provided for microbial conversion of D-limonene, and a theoretical basis is provided for biosynthesis and metabolic engineering modification of terpenoids; therefore, the method has a wide application prospect in the field of microbial fermentation.

The invention provides an NSS1-6 strain and application of the NSS1-6 strain in promoting germination of cistanche deserticola seeds. The strain of the growth-promoting bacterium NSS1-6 is classified and named as bacillus cereus, and is preserved in the China General Microbiological Culture Collection Center (CGMCC), and the preservation number is CGMCC NO.35464. The invention further discloses a preparation method of the growth-promoting bacterium NSS1-6. The invention also provides a microbial agent, and the unique effective component of the microbial agent is the NSS1-6 strain. According to the invention, the strain NSS1-6 which has a relatively good promoting effect on the germination rate and the aspirator rate of the cistanche seeds is found for the first time, and the strain shows relatively strong saline-alkaline tolerance.

The invention provides bacillus cereus, the bacillus cereus is named as bacillus cereus, the bacillus cereus is registered and preserved in Guangdong Microbial Culture Collection Center, and the preservation number is GDMCC NO: 66395. The invention further provides a synthetic flora, and the synthetic flora contains the bacillus cereus and the trichoderma asperellum MM7. The invention further provides a preparation method of the bacillus cereus and the trichoderma asperellum MM7. A synthetic flora constructed by the bacillus cereus and Trichoderma asperellum MM7 has a better effect of promoting the growth of banana plants, can be used for preparing a mixed bacterial agent or a microbial fertilizer, is beneficial to improving the economic benefits of the banana planting industry, and has a wide application space in green and sustainable development of the banana industry.

The invention discloses bacillus cereus with wide pH adaptability and application of the bacillus cereus in soil or underground water heavy metal remediation, the strain is named as bacillus cereus GS and preserved in the China Center for Type Culture Collection (the preservation number is CCTCC NO: M 20241939), and the bacillus cereus is named as bacillus cereus GS and preserved in the China Center for Type Culture Collection (CCTCC). The strain is high in environmental adaptability and high in remediation efficiency, can show good reduction and endurance capacity to various heavy metal pollutants under the wide environmental conditions that the pH ranges from 4 to 10 and the temperature ranges from 16 DEG C to 37 DEG C, and is particularly suitable for in-situ remediation of high-concentration contaminated soil; experimental results represented by hexavalent chromium show that the removal rate of the strain in 1000 mg / kg hexavalent chromium contaminated soil within 7 days can reach 70-100%, the dependence of an existing microbial remediation technology on a low-concentration and alkaline environment or a long remediation period is broken through, and an efficient solution is provided for green bioremediation of multiple types of heavy metal contaminated sites.

This invention relates to a strain of Bacillus cereus and its applications, specifically Bacillus cereus (… Bacillus cereus Bacillus cereus (W1) was deposited at the China Center for Type Culture Collection on July 21, 2022, with accession number CCTCC NO: M 20221147. Bacillus cereus W1 can grow and ferment high-yield butyric acid under non-strict anaerobic conditions, and has the advantages of strong oxygen tolerance and high butyric acid yield.

A phage composition and an use thereof in strengthening soil carbon fixation, said phage composition including three phages, namely, a polyvalent phage φYSZKP that attacks Klebsiella pneumoniae and Pseudomonas aeruginosa; a Bacillus cereus phage φYSZBA1; and a Bacillus cereus phage φYSZBA2. In the present invention, the phage composition is injected into the target soil, and an approach of strengthening soil carbon fixation by means of cooperation with the host bacteria through injection of AMGs from the phage is employed; at the same time, the phage has a function of regulating the soil microbial communities from bottom to top; a network of symbiosis with indigenous bacteria is further optimized, and the soil carbon fixation potential is improved. This technique is an environmental improvement technique that achieves a good carbon fixation effect, has a low price and is environment-friendly.

The invention discloses a method for preparing a soil conditioner for gardenia cultivation by using immobilized compound bacteria, which comprises the following steps: extracting gardenia lignin by using gardenia waste as a raw material, and preparing an inner-layer wall material to embed a mixed bacteria solution to obtain lignin inner-layer microcapsules; preparing an outer-layer wall material through modified chitosan, and compounding and curing the inner-layer microcapsule, the hydrogenated streptomyces liquid and the outer-layer wall material to obtain the immobilized composite microbial agent. And finally, mixing the immobilized complex microbial inoculant with matrixes such as leaf mold and peat soil to obtain the modifier. Ordered release of compound bacteria is realized through dual response, functional synergy of Rafissonia, Ochrobactrum and Streptomyces hydrogenii can be enhanced through population signal molecule modification, soil nutrients can be activated, effective iron can be chelated to solve the problem of iron deficiency yellowing of cape jasmine, soil pollutants and pesticide residues are degraded, and the soil quality is improved. Pathogenic bacteria reproduction is inhibited, and rhizosphere micro-ecological balance is maintained; plant hormones can be secreted to promote the growth of the cape jasmine, and the cape jasmine is converted into more components with high medicinal value.

The invention relates to the technical field of bactericides for crops, in particular to a bacillus cereus-containing bactericidal composition and application thereof in prevention and control of camellia oleifera anthracnose. The active ingredients of the bactericidal composition comprise bacillus cereus WR-12 and diflumetofen sulfanilamide. According to the bactericidal composition, the synergistic bactericidal effect is enhanced by compounding a biological fungicide and a chemical fungicide, the development of drug resistance of pathogenic bacteria is delayed, the negative influence of chemical pesticides on the environment is reduced, green and efficient prevention and control of camellia oleifera anthracnose are achieved, and sustainable development of the camellia oleifera industry is promoted.

The invention discloses a nucleic acid aptamer A4-CA2 with high affinity to vibrio parahaemolyticus and application of the nucleic acid aptamer A4-CA2, and belongs to the technical field of nucleic acid aptamers. The nucleotide sequence of the nucleic acid aptamer A4-CA2 with high affinity to vibrio parahaemolyticus is as shown in SEQ ID NO. 2. The invention further discloses application of the nucleic acid aptamer A4-CA2 with high affinity to the vibrio parahaemolyticus in detection of the vibrio parahaemolyticus and application of the nucleic acid aptamer A4-CA2 in preparation of products for detecting the vibrio parahaemolyticus. The affinity and dissociation constant of the nucleic acid aptamer A4-CA2 and the vibrio parahaemolyticus is 76.5 nM, and the nucleic acid aptamer A4-CA2 has high affinity, does not obviously bind to escherichia coli, staphylococcus aureus, aeromonas salmonicida and bacillus cereus, shows good specificity and is suitable for specific detection of the vibrio parahaemolyticus.

The invention relates to a cyanide degrading bacterium, a culture application thereof and a preparation containing the cyanide degrading bacterium, the cyanide degrading bacterium is bacillus cereus 2H-2, the bacterium is preserved in the China General Microbiological Culture Collection Center on March 18, 2024, and the preservation number is CGMCC No.30063. The cyanide degrading bacterium has the advantages that the cyanide degrading bacterium can be used for degrading cyanide, and the cyanide degrading bacterium can be used for degrading cyanide; the strain provided by the invention can stably degrade cyanide in a water body with heavy metals, has good settleability and is easy to separate and obtain.

The utility model discloses a kind of confirmation experimental devices for Bacillus cereus detection with wax sample, including transparent detection box, two magnetic seats are symmetrically installed in the upper end in transparent detection box, two magnetic seats upper end are fixed with glass slide by magnetic block, the upper end of transparent detection box is provided with several syringes, the lower end of syringe is connected with the upper end of hard long connecting pipe by pipe joint, the lower end of several long connecting pipes is located above glass slide, the uppermost of glass slide is also provided with horn head, the upper end of horn head is connected with air duct and air bag, the lower end of transparent detection box is set in the liquid collection box outside, compared with prior art, the beneficial effects of the utility model are: when carrying out confirmation experiment, each kind of reagent needed for dyeing is respectively loaded into different syringes, reagent is made to drop on glass slide by long connecting pipe by pushing syringe to carry out dyeing, air flow is generated by extruding air bag and acts on magnetic seat, realize air drying.

An antimicrobial peptide against Bacillus cereus, a preparation method therefor and a use thereof. The antimicrobial peptide against bacillus cereus has the general amino acid sequence formula of [KR]GG[KR]XC[FWY]C[KR]X[KR][FWY]C[LIAV]CXG[KR][KR], wherein the antimicrobial peptide XWC26 has a strong antibacterial activity against Bacillus cereus, efficiently inhibiting the spore germination of Bacillus cereus, and effectively controlling Bacillus cereus contamination in food. The antimicrobial peptide against bacillus cereus has the amino acid sequence of KGGKLCWCKNKWCICLGKR, with a molecular weight of 2224.79 Da, an isoelectric point of 9.70, and a net charge of 6. The antimicrobial peptide also has the advantages of high stability, reduced risk of drug resistance induction, high synthesis efficiency, low production costs, etc.

The invention belongs to the technical field of food poisoning rapid detection, and particularly relates to a method for detecting bacillus cereus vomitoxin in a serum sample, which comprises the following steps: (1) precipitating protein in the serum sample by adopting an extraction solvent in a purification tube to obtain a purification solution; (2) concentrating and redissolving the extracted and purified liquid; and (3) separating the redissolved purified liquid by adopting a liquid chromatographic column, detecting in an LC-MS / MS multi-reaction monitoring scanning mode, and quantitatively calculating the content of vomitoxin in bacillus cereus by adopting an internal standard method. The method disclosed by the invention is simple and convenient to operate, green and environment-friendly, accurate in qualitation and quantification and high in sensitivity, can be used as a mass spectrometric detection method for determining the bacillus cereus vomitoxin in a serum sample, can solve the problem of rapid treatment of food poisoning caused by the bacillus cereus vomitoxin in food, and meets detection requirements.

The invention discloses an antibacterial peptide RH22 and application thereof, the amino acid sequence of the antibacterial peptide RH22 is KLPFQR, and the molecular weight of the antibacterial peptide is 787.4704 Daltons. The antibacterial peptide disclosed by the invention has an obvious inhibition effect on bacillus cereus and can be used for preparing medicines, food preservatives or feed additives for preventing or inhibiting bacillus cereus infection.

The invention relates to the technical field of fermentation of traditional Chinese medicine compositions, in particular to application of a traditional Chinese medicine composition fermented by bacillus cereus PE-Z to improvement of animal immunity and improvement of intestinal flora and a method. According to the invention, a microbial bacillus cereus strain PE-Z suitable for fermentation of the traditional Chinese medicine composition disclosed by the invention is screened out; the traditional Chinese medicine composition is developed for improving the animal immunity, the traditional Chinese medicine composition is prepared from radix astragali, rhizoma atractylodis macrocephalae, red dates, poria cocos, pericarpium citri reticulatae, fructus crataegi, fructus mume, radix glycyrrhizae, rhizoma polygonati and / or malt, and after the traditional Chinese medicine composition is fermented through bacillus cereus PE-Z, the animal immunity can be remarkably improved; the richness of AKK bacteria and lactobacillus in the intestinal tract is obviously improved; after the traditional Chinese medicine composition is subjected to PE-Z fermentation, the polysaccharide content can be remarkably increased, an optimal culture medium is obtained after orthogonal optimization, and the fermentation process is optimized.

The application provides an island reef coral filler compaction pile and a construction method thereof. The construction method of the island reef coral filler compaction pile comprises the following steps: (1) using a weight to hammer the ground soil body to form a cylindrical hole; (2) first filling mixed sand and stone into the hole, the mixed sand and stone is composed of coral reef gravel and calcareous sand, then pouring a microbial curing liquid and a composite additive, the microbial curing liquid is composed of a composite bacterial liquid and seawater, the composite bacterial liquid is prepared from bacillus pasteurii, bacillus cereus and bacillus mucilaginosus, and the composite additive is composed of hydroxyl citronellal and eucalyptol; then using the weight to ram the filler; (3) repeating step (2) to ram and fill the pile hole to the ground to form the compaction pile. The construction method can significantly improve the quality of the compaction pile, significantly improve the single pile bearing capacity, and effectively reduce the relative usage amount of the microbial curing liquid.

The invention discloses an antibacterial peptide sesquidendrin and an application of the antibacterial peptide sesquidendrin. The invention specifically relates to eight sesquistigma polypeptide compounds. The compounds 1-8 show different degrees of antibacterial activity, the compound 2 has broad-spectrum antibacterial activity, the minimum inhibitory concentration of the compound 2 to staphylococcus aureus ATCC 6538 and bacillus cereus CMCC (B) 63301 reaches 20 [mu] g / mL, and the MIC value of the compound 2 to enterococcus faecium 160119481, salmonella typhimurium CMCC (B) 50071 and salmonella typhimurium CMCC (B) 50115 is 40 [mu] g / mL. As staphylococcus, bacillus, enterococcus and salmonella bacteria are common food-borne pathogenic bacteria which seriously threaten food safety, the compounds 1-8 can be used for preparing novel antibacterial peptides which are applied to the field of food safety preservation.

This invention belongs to the field of microbial fermentation technology, specifically relating to a method for preparing microbial inoculants through mixed fermentation of Bacillus subtilis and Bacillus cereus. The method includes: separately activating Bacillus subtilis and Bacillus cereus to obtain a seed culture; adding a basic fermentation medium to a fermenter, first inoculating Bacillus subtilis, and then inoculating Bacillus cereus after it enters the mid-logarithmic growth phase, achieving sequential mixed inoculation; employing segmented temperature control, two-stage pH control, and dynamic sugar feeding during fermentation, while simultaneously adding malic acid in a coupled manner between 20 and 35 hours to alleviate oxidative stress; collecting the cells after fermentation, adding a protective agent, and drying to obtain the inoculant. This invention, through the synergistic effect of sequential inoculation and malic acid coupled feeding, achieves an effective viable cell count of up to 1.0 × 10⁻⁶. 13 With a CFU / mL or higher, the spore formation rate is ≥89%, which is significantly better than the existing mixed-strain fermentation process.

The invention belongs to the technical field of microbial prevention and control of plant diseases and insect pests, and particularly discloses a plant disease biocontrol bacillus cereus strain LCBC246 which is preserved in the China General Microbiological Culture Collection Center (CGMCC), and the preservation number of the strain is CGMCC No.33323. The invention further discloses a preparation method of the plant disease biocontrol bacillus cereus strain LCBC246. The LCBC246 strain provided by the invention is a gram-positive bacterium, has a very strong bacteriostatic effect on colletotrichum fucicola, the bacteriostatic rate is 92.1%, the disease control rate on the colletotrichum fucicola is 90.8%, and the LCBC246 strain has a very good inhibitory effect on other important plateau characteristic crop pathogenic bacteria and also has a remarkable crop growth promoting property. The bacterial inhibition rates of fermentation liquor and volatile substances of the strain LCBC246 on colletotrichum gloeosporioides of macadamia nuts are 62.70% and 76.58% respectively; the bacillus amyloliquefaciens can generate sterilization metabolites such as cellulase, amylase and siderophore, and has a very strong broad-spectrum bacteriostatic effect on various plant pathogenic fungi. Therefore, the strain LCBC246 and the metabolite thereof have the application value of developing bacterial biopesticides.

A method for decomposition of keratin material, e. g. mixture of feathers, is disclosed. The method comprises treating the keratin material with Bacillus cereus KB043 strain, subsequently with sodium hydroxide and potassium hydroxide, and finally with neutralizing acid. The end product is intended for use as an amino acid fertilizer.

The present application relates to a kind of lignocellulose as raw material fermentation production butyric acid method, comprising the following steps: (1) lignocellulose raw material is pretreated, enzymolysis, filtration obtains enzymolysis liquid;(2) the enzymolysis liquid is detoxified, separates out the precipitate after pH adjustment as fermentation medium;(3) the seed liquid of bacillus cereus (W1) Bacillus cereus ) W1 is inoculated into fermentation medium in non-strict anaerobic environment, and fermentation production butyric acid.The present application uses lignocellulose as raw material, utilizes bacillus cereus (W1) Bacillus cereus ) W1 in non-strict anaerobic environment fermentation production butyric acid, with butyric acid yield high, product selectivity is high, fermentation cost is low and so on.

The invention discloses a hydrogel and probiotic compounded microbial inoculum composition and a vegetable planting matrix, a three-dimensional gel carrier is used for embedding a microbial inoculum, the carrier integrates mechanical properties and thallus protection and can maintain flora activity for a long time, bacillus thuringiensis has a low-oxygen survival characteristic after being subjected to Fnr enhanced expression, and the microbial inoculum composition has the advantages that the microbial inoculum composition has a low-oxygen survival characteristic after being subjected to Fnr enhanced expression; the microbial agent composition can survive for a long time in a low-oxygen environment of a carrier embedding environment, and is cooperated with bacillus cereus, azotobacter chroococcum and bacillus megatherium to promote the growth of plants such as vegetables and the like and directionally kill pathogenic microorganisms such as root-knot nematode. Corresponding pests can be killed in a green and targeted manner, the growth of plants is promoted, and the yield is increased.

The invention relates to a biocontrol inoculant composition and application thereof.The biocontrol inoculant composition comprises bacillus subtilis z373 and bacillus cereus z266, the total viable bacterium concentration of the biocontrol inoculant composition is 4 * 10 < 7 >-8 * 10 < 7 > CFU / mL, the viable bacterium number ratio of the bacillus subtilis to the bacillus cereus is (1: 10)-(10: 1), and the biocontrol inoculant composition is stable in soybean rust prevention and treatment effect and low in cost. The control effect is greater than 90%; the soybean antibacterial agent has excellent antibacterial performance, can promote soybean growth, is non-toxic and safe to people and livestock, is environment-friendly, and has important application significance and value.

The invention belongs to the technical field of microorganisms, and particularly relates to rhizoma polygonati endophytic bacillus cereus and application thereof. The rhizoma polygonati endophytic bacillus cereus SK6 is separated and screened from rhizoma polygonati, the bacillus cereus is preserved in the China General Microbiological Culture Collection Center, and the preservation number is CGMCC (China General Microbiological Culture Collection Center) No.36060. The bacillus cereus strain provided by the invention has excellent exopolysaccharide synthesis capability, and the exopolysaccharide content in the fermentation liquor obtained by fermenting the bacillus cereus at 37 DEG C for 24 hours is up to 44.22 g / L.