24 results about "Bacillus cereus" patented technology

This invention relates to a strain of Bacillus cereus and its applications, specifically Bacillus cereus (… Bacillus cereus Bacillus cereus (W1) was deposited at the China Center for Type Culture Collection on July 21, 2022, with accession number CCTCC NO: M 20221147. Bacillus cereus W1 can grow and ferment high-yield butyric acid under non-strict anaerobic conditions, and has the advantages of strong oxygen tolerance and high butyric acid yield.

The utility model discloses a kind of confirmation experimental devices for Bacillus cereus detection with wax sample, including transparent detection box, two magnetic seats are symmetrically installed in the upper end in transparent detection box, two magnetic seats upper end are fixed with glass slide by magnetic block, the upper end of transparent detection box is provided with several syringes, the lower end of syringe is connected with the upper end of hard long connecting pipe by pipe joint, the lower end of several long connecting pipes is located above glass slide, the uppermost of glass slide is also provided with horn head, the upper end of horn head is connected with air duct and air bag, the lower end of transparent detection box is set in the liquid collection box outside, compared with prior art, the beneficial effects of the utility model are: when carrying out confirmation experiment, each kind of reagent needed for dyeing is respectively loaded into different syringes, reagent is made to drop on glass slide by long connecting pipe by pushing syringe to carry out dyeing, air flow is generated by extruding air bag and acts on magnetic seat, realize air drying.

This invention belongs to the field of microbial fermentation technology, specifically relating to a method for preparing microbial inoculants through mixed fermentation of Bacillus subtilis and Bacillus cereus. The method includes: separately activating Bacillus subtilis and Bacillus cereus to obtain a seed culture; adding a basic fermentation medium to a fermenter, first inoculating Bacillus subtilis, and then inoculating Bacillus cereus after it enters the mid-logarithmic growth phase, achieving sequential mixed inoculation; employing segmented temperature control, two-stage pH control, and dynamic sugar feeding during fermentation, while simultaneously adding malic acid in a coupled manner between 20 and 35 hours to alleviate oxidative stress; collecting the cells after fermentation, adding a protective agent, and drying to obtain the inoculant. This invention, through the synergistic effect of sequential inoculation and malic acid coupled feeding, achieves an effective viable cell count of up to 1.0 × 10⁻⁶. 13 With a CFU / mL or higher, the spore formation rate is ≥89%, which is significantly better than the existing mixed-strain fermentation process.

This invention relates to a compound microbial agent for the prevention and control of tobacco black shank disease, belonging to the field of biotechnology. The compound microbial agent comprises a suspension of *Pseudomonas aeruginosa* 232, *Bacillus cereus* 183, and *Mesophytes stolonifer* 161 in a volume ratio of 3:1:3, wherein the effective viable count in each of the aforementioned three bacterial suspensions is not less than 1 × 10⁻⁶. 9 CFU / mL. The compound biocontrol agent provided by this invention can effectively control tobacco black shank disease. Experimental verification shows that after 8 weeks of application of this agent to tobacco seedlings, the biocontrol effect against tobacco black shank disease can reach 33.34%.

This invention belongs to the field of microbial technology and discloses a synthetic microbial community for enhancing biological nitrogen fixation in karst grasslands, its construction method, and its application. The synthetic microbial community includes... Sinorhizobium americanum , Sionrhizobium sp. as well as Bacillus cereus Wherein: the Sinorhizobium americanum The accession number is GDMCC NO: 67796; Sionrhizobium sp. The accession number is GDMCC NO: 67797; Bacillus cereus The accession number is GDMCC NO: 67798. For leguminous forage grasses that require repeated sowing, the seeds are treated with a synthetic microbial community before being sown in the field; for perennial leguminous forage grasses that have already been planted, the synthetic microbial community is diluted with water and sprayed onto the surface of adjacent leguminous forage grasses. This invention screens out suitable and efficient root nodule endophytic bacteria in karst areas and constructs a synthetic microbial community to enhance the biological nitrogen fixation of leguminous forage grasses. It clarifies the technical details of the practical application of the synthetic microbial community in the field. The application of this technology can significantly improve the biological nitrogen fixation rate of leguminous forage grasses, significantly reduce the amount of nitrogen fertilizer used in forage grasses, and significantly increase forage grass yield.

This invention provides a compound microbial agent, the compound microbial agent comprising methyltrophic Bacillus (Bacillus) Bacillus methylotrophicus ), with accession number CGMCC No. 20908 and Bacillus cereus ( Bacillus cereus The compound microbial agent, with accession number CGMCC No.24485 and a ratio of methyltrophic Bacillus to Bacillus cereus of 1~5:1~5, can be used to improve the cold and drought resistance of fruit trees.

The application discloses a method for high-efficiency degradation of high-salinity wastewater by salt-tolerant bacteria in a composite inorganic salt system, and relates to the technical field of biological treatment of high-salinity industrial wastewater.The method realizes directional strengthening of the salt-tolerant performance of three strains of Staphylococcus xylosus, Alcaligenes faecalis and Bacillus cereus by a gradient-increasing NaCl domestication method.Under the condition of 40-50 g / L high salinity, the growth amount of the domesticated strains is increased by 30-40% compared with that of the non-domesticated strains, effectively relieving the osmotic pressure stress and metabolic enzyme activity inhibition of the microorganisms in the high-salinity environment.Meanwhile, the composite salt-tolerant bacteria agent is compounded based on functional complementation, and the agent has the abilities of efficient denitrification, phosphorus removal and organic matter degradation, and can adapt to the composite inorganic salt system of NaCl, CaCl2 and MgSO4 alone or in combination, and can realize synchronous and efficient removal of COD, ammonia nitrogen, total nitrogen and total phosphorus in a wide salinity range of 10-80 g / L.The method breaks through the technical limitation that the existing salt-tolerant bacteria only adapt to a single salt system and have single degradation function, and greatly widens the applicable scenarios of the biological method in the treatment of high-salinity wastewater.

The application relates to an anti-Bacillus cereus nanobody and application thereof, the nanobody comprising a framework region FR and a complementarity determining region CDR, wherein the complementarity determining region CDR is CDR1 with an amino acid sequence as shown in SEQ ID NO. 6, CDR2 with an amino acid sequence as shown in SEQ ID NO. 7 and CDR3 with an amino acid sequence as shown in SEQ ID NO. 8. The nanobody can recognize and combine with Bacillus cereus, and has the advantages of easy expression and high expression efficiency, so that the nanobody can be applied to an enzyme-linked immunoassay method for detecting Bacillus cereus.

This invention belongs to the field of pesticide and fertilizer technology, specifically relating to a pesticide and fertilizer for controlling underground pests in sugarcane. A microbial pesticide and fertilizer is provided, comprising a mixture of *Bacillus cereus* fermentation broth and *Bacillus cereus* fermentation broth; wherein the concentration of the *Bacillus cereus* fermentation broth is 2.5 × 10⁻⁶. 8 Spores / g; the concentration of the Bacillus cereus fermentation broth is 1.0 × 10⁻⁶. 8 CFU / g. This invention's microbial fertilizer is a mixture of *Bacillus cereus* fermentation broth and *Bacillus cereus* fermentation broth. The toxicity of the two fermentation broths and their mixture against sugarcane underground pests was determined using an indoor immersion method. The results showed that when the two were mixed at a mass ratio of 1:2-8, they had a synergistic effect on controlling sugarcane underground pest larvae. Compared with using *Bacillus cereus* fermentation broth or *Bacillus cereus* fermentation broth alone, this method improves the control effect against sugarcane underground pests, which is of great significance for the development of microbial agents and fertilizers for controlling sugarcane underground pests.

The present application belongs to the technical field of biological fermentation, and relates to a culture medium-free biological conversion method of citrus essential oil based on compound strains, comprising the following steps: 1, preparing wet bacteria bodies of Bacillus cereus GJ-02 and Pseudomonas nitroreducens GJ-01 respectively; 2, compounding the obtained two kinds of wet bacteria bodies; 3, suspending the compounded wet bacteria bodies in a buffer solution, and adding citrus essential oil to perform a culture medium-free biological conversion reaction; and 4, performing post-treatment on the obtained reaction liquid to recover a perfume product; the culture medium in the conversion stage is omitted, the process is simple, the cost is low, and there is no pollution, and the product synergism is realized through strain compounding, the obtained perfume has full and coordinated aroma, and is particularly suitable for tobacco flavoring, food and daily chemical perfume fields.

This invention discloses a Bacillus cereus bacteriophage, WAH1, and its applications. The Bacillus cereus bacteriophage WAH1, with accession number GDMCC No:67732-B1, does not carry drug resistance or virulence factors, can specifically lyse Bacillus cereus, and has a broad host spectrum. This bacteriophage possesses a short latency period and high burst capacity, enabling efficient lysis of host bacteria. Furthermore, WAH1 exhibits strong tolerance to factors such as temperature, pH, and osmotic pressure, demonstrating strong environmental adaptability and resistance to inactivation during application. This bacteriophage shows significant inhibitory effects on the target bacteria in liquid culture media, milk, and rice. In summary, WAH1 bacteriophage shows promising application prospects in controlling Bacillus cereus contamination in food.

This invention discloses a method for promoting rice growth using a compound microbial agent and humic acid. Humic acid and the compound microbial agent are added sequentially to rice-grown soil. The amount of humic acid added is 0.5-2% of the soil mass, preferably 1% of the soil mass. The solid-liquid ratio of humic acid to the compound microbial agent (g:ml) is 0.5-1.1:15-30, preferably 1:20. The compound microbial agent is obtained by mixing any two or more monofunctional bacteria in equal volume ratios. The monofunctional bacteria include *Bacillus amyloliquefaciens* FH1, *Bacillus shortwave diversicolor* NH1, *Glucosidobacterium tumefaciens* QZR14, *Bacillus cereus* S112, and *Pantothecinus flocculationensis*. 、 The compound microbial agent of this invention, along with humic acid, can effectively promote rice growth, optimize soil microbial community and function, and provide a scientific basis for the research and application of new compound microbial fertilizers, thus contributing to the development of sustainable agriculture.

The application belongs to the technical field of biology, and particularly relates to a bacillus cereus for preventing and treating root-knot nematode and application thereof. The bacillus cereus is named as bacillus cereus ZWL-B-24, is preserved in Guangdong Microbial Culture Collection Center (GDMCC) with an address of 5th floor, building 59, Guangdong Academy of Microbiology, 100, Middle Junli Street, Guangzhou, and a preservation number of GDMCC No: 63680, and a preservation time of July 24, 2023. The bacillus cereus obtained in the application is derived from sweet potato soil, has a strong soil colonization capacity, and due to a long-term coexistence with sweet potato root systems, mutual symbiosis, a series of similar survival mechanisms are formed, and the bacillus cereus is applied to the prevention and treatment of sweet potato root-knot nematode disease, and is more safe and reliable for the growth, edible and processing products of sweet potato.

This invention discloses a compound microbial agent for the efficient degradation of lignin in tobacco straw, its preparation method, and its application. The compound microbial agent is composed of *Bacillus velezensis* L13 and *Bacillus cereus* L15. The compound agent contains at least 1 × 10⁻⁶ viable bacteria in each component. 7 The above two strains, each containing CFU / mL, were mixed in a 1:1 volume ratio. This invention also provides a method for preparing this compound microbial agent and its application in degrading lignin in tobacco straw and producing organic fertilizer. Furthermore, this invention provides a method for producing organic fertilizer, which involves mixing crushed tobacco straw and sheep manure at a mass ratio of 7:3, inoculating with the compound microbial agent, adjusting the carbon-to-nitrogen ratio to 25:1 and the moisture content to 60%-65%, and then aerobically fermenting at 40-60℃ for 50-60 days. This compound microbial agent can efficiently and synergistically degrade lignocellulose in tobacco straw, significantly accelerate the composting process, improve the quality of compost products, and realize the resource utilization of agricultural waste.

This invention discloses a microbial community for improving the salt tolerance of economic plants and its application. The compound microbial community of this invention contains *Burkholderia* spp. HNMD5-1, *Priscilla megaterium* 3475-4, *Bacillus glabrata* 3174-3, and *Bacillus cereus* NSTJ2Y-4. Experiments show that this compound microbial community can be used to improve the salt tolerance of tiger nuts, soybeans, and Chinese cabbage, and promote plant growth. Therefore, the compound microbial community of this invention has important application value in the cultivation and production of tiger nuts, soybeans, and Chinese cabbage.

This invention discloses a compound microbial agent for fecal degradation and deodorization, its preparation method, and its application. The agent comprises a core degrading bacterial group (Bacillus cereus), a nitrogen-retaining and deodorizing bacterial group (Bacillus nitrosporioides BM62), an antibiotic-reducing bacterial group (Gottfried bacteria TW007), a low-temperature-adapting bacterial group (Cryptobrachium frutescens TW009), and basic microecological regulating bacterial groups (Lactobacillus, yeast, and photosynthetic bacteria). The agent also includes auxiliary materials such as water, brown sugar, potassium dihydrogen phosphate, bovine bone peptone, yeast powder, and wheat bran. The preparation method includes nutrient base preparation, organic nitrogen source compounding, stepwise activation, and sealed maturation steps. This invention's agent achieves multi-target synergistic treatment of fecal waste through the synergistic effect of functional bacterial groups. The agent is low-cost and simple to prepare, suitable for various scenarios such as toilet septic tanks, livestock farm manure composting, and wastewater purification. The treated fecal residue can be directly used as bio-organic fertilizer, exhibiting significant environmental and economic benefits.

The present invention provides a composite biocontrol bacterial seed treatment agent containing Streptococcus thermophilus, a preparation method and an application, belonging to the technical field of biological pesticides. The seed treatment agent is a solid powder consisting of 80% to 90% effective active component and 10% to 20% auxiliary materials. The effective active component is an equal-proportion composite bacterial fermentation broth concentrate solidification product of Bacillus subtilis, Bacillus cereus, Bacillus thuringiensis and Streptococcus thermophilus. In the present invention, the fermentation concentrate of multiple bacterial strains is mixed with a carrier and spray- dried to obtain the composite bacterial fermentation broth concentrate solidification product; then the solidification product is mixed with the auxiliary materials to obtain the present invention.

This invention proposes a droplet digital PCR method for simultaneously detecting the quantity of four foodborne pathogens. The steps include: 1. Preparing the DNA sample to be tested; 2. Preparing primers and probes for the four bacteria to be tested; the four bacteria to be tested are Staphylococcus aureus, Listeria monocytogenes, Salmonella typhi, and Bacillus cereus; 3. Using a ddPCR amplification reaction system, which includes the primers and probes for the four bacteria to be tested in step 2, amplifying the DNA sample to be tested in step 1, detecting the amplification products, and obtaining the quantitative results of the four bacteria. This invention can simultaneously detect four pathogenic microorganisms: Staphylococcus aureus, Listeria monocytogenes, Salmonella typhi, and Bacillus cereus. It establishes a precise, rapid, and absolute quantitative detection system for the simultaneous detection of these four pathogens using multiplex ddPCR technology, exhibiting high specificity, high precision, and strong repeatability.

This invention belongs to the field of biomedical engineering and ophthalmic anti-infective therapy technology, specifically relating to a bacteriophage XP2 for treating Bacillus cereus endophthalmitis and its applications. Bacteriophage XP2 was deposited on November 18, 2025, at the China General Microbiological Culture Collection Center (CGMCC), with accession number CGMCC No. 47001. The bacteriophage provided by this invention exhibits high specificity in killing host bacteria, acting only against the host bacteria without disrupting normal flora or inducing widespread drug resistance. Simultaneously, the bacteriophage itself is non-toxic to normal retinal function and demonstrates excellent preservation of retinal function, even superior to vancomycin treatment, with a shorter onset time than vancomycin, approximately 30 minutes. This invention confirms the effectiveness of bacteriophage XP2 in directly reducing the bacterial load in Bacillus cereus-induced endophthalmitis and indirectly controlling inflammatory damage. By rapidly killing bacteria, XP2 minimizes neutrophil-induced inflammatory responses while protecting retinal structure and function.

The application belongs to the technical field of microbial culture, and particularly relates to a bacillus cereus B5 and application thereof. The bacillus cereus B5 is isolated from an alpine environment of Qinghai-Tibet Plateau, and the preservation number is GDMCC No: 65417. The strain and secreted metabolites thereof can improve the mycelium formation rate of biocontrol fungi and insect resource fungi, and accelerate the formation process of conidia. Indoor detection is conducted on the influence of B5 on the growth of insect pathogenic fungi and the efficiency of killing host insects, and the results show that B5 can improve the mycelium formation rate of the tested strains, and simultaneously accelerate the growth rate of conidia, thereby providing technical support for fungal insecticides and artificial cultivation of fungal resource industry.