Compound microorganism deodorization fungicide as well as preparation method and application thereof

Abstract

The invention relates to a compound microorganism deodorization fungicide as well as a preparation method and application thereof, and belongs to the technical field of microorganism application. The invention provides a compound microorganism deodorization bacterium agent. The compound microorganism deodorization bacterium agent is prepared from bacillus cereus GXMU-Q5.3, streptomyces beihaiensis GXMU-J15 and lactobacillus paracasei GXMU-G1, the viable count ratio of the bacillus cereus GXMU-Q5.3 to the streptomyces beihaiensis GXMU-J15 to the lactobacillus paracasei GXMU-G1 is (1 to 2): (1 to 2): (1 to 2). The strain of the composite microbial deodorant agent is screened from soil and water at the stacking place of the marine product processing waste, the decomposition and deodorization effects on the marine product processing waste are obvious, the bacteria have a synergistic effect, and the deodorization effect of the composite microbial agent is improved by 40% or above compared with that of a single strain; the cost is low, the efficiency is high, the application range is wide, the deodorization period is short, and the safety is high.

Description

Technical Field

[0001] The present invention relates to the technical field of microbial application, and in particular to a composite microbial deodorizing agent and a preparation method and application thereof. Background Art

[0002] my country has vast sea areas and huge seafood production. In the process of seafood processing, fish heads, fish skins, fish bones, fish viscera, shrimp heads, shrimp shells, shrimp tails, crab shells and other seafood processing wastes are generated. Seafood processing wastes are rich in chitin, protein, peptides, lipids and other organic matter, but they have a bad smell and are not eaten by livestock, so most of them are directly discarded. If they are not properly processed, they will not only cause serious pollution to the marine and terrestrial environments, but also cause serious waste of resources. Using microorganisms to degrade seafood processing wastes into plant-usable substances such as chitosan oligosaccharides, amino acids, and active peptides, and through the compounding and chelation of different degradation products, a series of products such as compound crop-specific organic fertilizers, water-soluble fertilizers or soil conditioners are developed according to the growth needs of various plants and the soil conditions in various places. This can increase the added value of seafood processing wastes and is expected to produce significant economic, social and ecological benefits.

[0003] However, during the degradation of seafood processing waste raw materials, smelly gases will be generated, mainly nitrogen-containing compounds (ammonia, amines, phthalamides, etc.), sulfur-containing compounds (hydrogen sulfide, thiols, sulfur dioxide, thioether compounds, etc.), halogens and their derivatives (chlorine, halogenated hydrocarbons, etc.) and oxygen-containing organic compounds (aromatic rings such as phenols, alcohols, organic acids, aldehydes and ketones, etc.). The generation of a large amount of smelly gases will not only pollute the environment and cause harm to human health, but also cause the loss of nitrogen and sulfur elements in the raw materials.

[0004] At present, the commonly used methods of deodorization can be divided into three categories: physical, chemical and biological deodorization. The physical deodorization method mainly uses some adsorbents or masking agents to absorb odorous substances into porous carriers, temporarily adsorbing and covering up part of the odor, but cannot deodorize fundamentally; and the adsorbent is easily saturated and difficult to desorb, resulting in the inability to reuse, causing secondary pollution to the environment. The chemical deodorization method mainly uses oxidants to oxidize some odorous components, which has the characteristics of strong targeting and quick effect. However, due to its singleness in removing odorous gases, when it encounters complex odorous gas components, its deodorization effect will be greatly reduced. At the same time, the cost of use is high, and it is easy to cause secondary pollution to the environment. The biological deodorization method mainly uses the physiological metabolism of microorganisms to decompose and transform odorous components, or block the generation of odorous components; compared with physical and chemical methods, it has the advantages of thorough deodorization, high efficiency, and not easy to produce secondary pollution. It has broad application prospects and has become a hot spot in the research and application of odor prevention and control at home and abroad.

[0005] Many existing deodorizing bacteria have shown good deodorizing effects in tests at room temperature, but the salt content of seafood processing waste is high, and during composting, the high temperature stage is the key stage for high temperature aerobic composting of organic solid waste. Most organic matter is oxidized and decomposed in this process, and the release of ammonia and hydrogen sulfide is the highest. However, the current microbial deodorizing bacteria have poor salt and high temperature resistance, and are not very applicable in the field of organic composting of seafood processing waste. Therefore, for the deodorization of seafood processing waste, a new high-efficiency deodorant still needs to be developed to solve the odor pollution problem caused by the fertilizer production process of seafood processing waste.

[0006] Based on this, the present invention is proposed. Summary of the invention

[0007] The purpose of the present invention is to provide a composite microbial deodorant and a preparation method and application thereof, so as to solve the problem that the microbial deodorants in the prior art have poor salt and high temperature resistance and are not highly applicable in the field of organic composting of seafood processing waste.

[0008] In order to achieve the above-mentioned object of the invention, the present invention provides the following technical solutions:

[0009] The invention provides a composite microbial deodorizing agent, comprising Bacillus cereus GXMU-Q5.3, Streptomyces niger GXMU-J15 and Lactobacillus paracasei GXMU-G1;

[0010] The ratio of the number of live bacteria of Bacillus cereus GXMU-Q5.3: Streptomyces cerevisiae GXMU-J15: Lactobacillus paracasei GXMU-G1 is 1-2:1-2:1-2;

[0011] The Latin name of the Bacillus cereus GXMU-Q5.3 is Bacillus cereus GXMU-Q5.3, which is deposited in Guangdong Microbiological Culture Collection Center with a deposit number of GDMCC NO: 62890, a deposit date of October 14, 2022, and a deposit address of 5th Floor, Building 59, No. 100 Xianlie Middle Road, Guangzhou;

[0012] The Latin name of the Beihai Streptomyces GXMU-J15 is Streptomyces sp. GXMU-J15, which is deposited in Guangdong Microbiological Culture Collection Center with the deposit number GDMCC NO: 63901, the deposit date is October 20, 2023, and the deposit address is 5th Floor, Building 59, No. 100 Xianlie Middle Road, Guangzhou;

[0013] The Latin name of the Lactobacillus paracasei GXMU-G1 is Lacticaseibacillus paracaseiGXMU-G1, which is preserved in the Guangdong Provincial Microbiological Culture Collection Center with a preservation number of GDMCCNO: 63900, a preservation date of October 20, 2023, and a preservation address of 5th Floor, Building 59, No. 100 Xianlie Middle Road, Guangzhou.

[0014] The invention provides application of the composite microbial deodorizing agent in preparing deodorizing products.

[0015] The present invention provides a liquid composite microbial deodorant, which is prepared from the composite microbial deodorant, wherein the total number of viable bacteria of Bacillus cereus GXMU-Q5.3, Streptomyces niger GXMU-J15 and Lactobacillus paracasei GXMU-G1 in the liquid composite microbial deodorant is greater than 4×10 8 CFU / mL.

[0016] The present invention provides a method for preparing the liquid composite microbial deodorant, comprising the following steps:

[0017] (1) activating Bacillus cereus GXMU-Q5.3, Streptomyces niger GXMU-J15 and Lactobacillus paracasei GXMU-G1 respectively to obtain activated Bacillus cereus GXMU-Q5.3, Streptomyces niger GXMU-J15 and Lactobacillus paracasei GXMU-G1;

[0018] (2) fermenting and culturing the activated Bacillus cereus GXMU-Q5.3, Streptomyces spp. GXMU-J15 and Lactobacillus paracasei GXMU-G1 at a temperature of 30-35° C. to obtain Bacillus cereus GXMU-Q5.3 fermentation liquid, Streptomyces spp. GXMU-J15 fermentation liquid and Lactobacillus paracasei GXMU-G1 fermentation liquid;

[0019] (3) The fermentation liquid of Bacillus cereus GXMU-Q5.3, the fermentation liquid of Streptomyces niger GXMU-J15 and the fermentation liquid of Lactobacillus paracasei GXMU-G1 obtained in step (2) are mixed according to the ratio of viable bacteria count to obtain a liquid composite microbial deodorant.

[0020] The present invention provides a solid composite microbial deodorant, which is prepared from the composite microbial deodorant, wherein the total number of viable bacteria of Bacillus cereus GXMU-Q5.3, Streptomyces niger GXMU-J15 and Lactobacillus paracasei GXMU-G1 in the solid composite microbial deodorant is greater than 1×10 9 CFU / g.

[0021] The present invention provides a method for preparing the solid composite microbial deodorant, comprising the following steps:

[0022] (1) activating Bacillus cereus GXMU-Q5.3, Streptomyces niger GXMU-J15 and Lactobacillus paracasei GXMU-G1 respectively to obtain activated Bacillus cereus GXMU-Q5.3, Streptomyces niger GXMU-J15 and Lactobacillus paracasei GXMU-G1;

[0023] (2) fermenting and culturing the activated Bacillus cereus GXMU-Q5.3, Streptomyces spp. GXMU-J15 and Lactobacillus paracasei GXMU-G1 at a temperature of 30-35° C. to obtain Bacillus cereus GXMU-Q5.3 fermentation liquid, Streptomyces spp. GXMU-J15 fermentation liquid and Lactobacillus paracasei GXMU-G1 fermentation liquid;

[0024] (3) collecting the Bacillus cereus GXMU-Q5.3 bacterial cells in the fermentation broth of step (2), drying, and crushing to obtain Bacillus cereus GXMU-Q5.3 bacterial powder;

[0025] (4) collecting the bacterial cells in the fermentation broth of Streptomyces beihaiensis GXMU-J15 and the fermentation broth of Lactobacillus paracasei GXMU-G1, freeze-drying and pulverizing to obtain bacterial powder of Streptomyces beihaiensis GXMU-J15 and bacterial powder of Lactobacillus paracasei GXMU-G1;

[0026] (5) The Bacillus cereus GXMU-Q5.3 powder, the North Sea Streptomyces GXMU-J15 powder and the Lactobacillus paracasei GXMU-G1 powder are mixed according to the ratio of viable bacteria count to obtain a solid composite microbial deodorant.

[0027] Preferably, the drying temperature in step (3) is 50-70° C., and the crushed particle size is 100-300 mesh;

[0028] The freeze-drying temperature in step (4) is -75 to -85°C, the freeze-drying time is 5 to 7 hours, and the particle size of the crushed product is 100 to 300 meshes.

[0029] The present invention also provides the use of the liquid composite microbial deodorant or the liquid composite microbial deodorant prepared by the preparation method or the solid composite microbial deodorant or the solid composite microbial deodorant prepared by the preparation method in removing odorous substances; the odorous substances include one or more of nitrogen-containing compounds, sulfur-containing compounds, halogens and their derivatives, and oxygen-containing compounds;

[0030] The nitrogen-containing compound includes ammonia, amine or phthalamide;

[0031] The sulfur-containing compound includes hydrogen sulfide, mercaptan, sulfur dioxide or thioether;

[0032] The halogen and its derivatives include chlorine or halogenated hydrocarbons;

[0033] The oxygen-containing compound includes phenol, alcohol, organic acid or aldehyde or ketone.

[0034] The present invention also provides a method for removing odorous substances, which comprises mixing a solid composite microbial deodorizing agent or a liquid composite microbial deodorizing agent with water and spraying the mixture on a place where the odorous substances are generated;

[0035] The liquid composite microbial deodorant is the liquid composite microbial deodorant or the liquid composite microbial deodorant prepared by the preparation method;

[0036] The solid composite microbial deodorizing agent is the solid composite microbial deodorizing agent described above or the solid composite microbial deodorizing agent prepared by the preparation method described above.

[0037] Preferably, the mass ratio of the solid composite microbial deodorant to water is 1:23-27; the mass ratio of the liquid composite microbial deodorant to water is 1:8-12;

[0038] The odorous substances include one or more of nitrogen-containing compounds, sulfur-containing compounds, halogens and their derivatives, and oxygen-containing compounds;

[0039] The nitrogen-containing compound includes ammonia, amine or phthalamide;

[0040] The sulfur-containing compound includes hydrogen sulfide, mercaptan, sulfur dioxide or thioether;

[0041] The halogen and its derivatives include chlorine or halogenated hydrocarbons;

[0042] The oxygen-containing compound includes phenol, alcohol, organic acid or aldehyde ketone;

[0043] The places where the odorous substances are produced include seafood processing waste, garbage stations, public toilets or livestock and poultry farms.

[0044] The present invention has the following technical effects and advantages:

[0045] (1) The strains used in the present invention are naturally screened from the soil and water bodies where seafood processing waste is piled up, and have obvious effects on the decomposition and odor suppression of seafood processing waste. Bacillus cereus GXMU-Q5.3 can effectively inhibit the decomposition and conversion of nitrogen-containing organic matter to release ammonia, and can also secrete chitinase to degrade chitin components such as shrimp and crab shells in seafood; North Sea Streptomyces GXMU-J15 can secrete chitinase, protease, etc. to degrade chitin, protein and other components, and at the same time can use the secreted metabolites to inhibit the growth of miscellaneous bacteria and reduce the generation of odorous gases; Lactobacillus paracasei GXMU-G1 can use organic matter in seafood processing waste to produce organic acids, which can effectively inhibit the release of ammonia.

[0046] (2) The composite microbial deodorant of the present invention has a synergistic effect with Bacillus cereus GXMU-Q5.3, Streptomyces cerevisiae GXMU-J15 and Lactobacillus paracasei GXMU-G1. The composite microbial deodorant has a 40% higher deodorizing effect than a single strain. The composite microbial deodorant can effectively reduce the odor substances in seafood processing waste, including indole, NH 3 , H 2 S and E. coli content, reduce the emission of harmful substances, avoid the loss of available components in seafood processing waste, and can efficiently convert proteins in seafood processing waste into small molecule peptides and amino acids, solving the problem of difficult efficient conversion and full utilization of seafood waste, and the processing time is short, with the advantages of simplicity, low cost, mild reaction conditions, and better economy. It can be used for resource treatment of seafood processing waste and deodorization of garbage stations, public toilets and livestock and poultry farms.

[0047] (3) The composite microbial deodorant provided by the present invention has low preparation cost, high efficiency, wide adaptability and short deodorization cycle. The composite microbial deodorant is prepared by the cooperation of multiple microorganisms. By configuring the culture solution and active bacterial solution in advance, different microbial compositions can be obtained by one fermentation, which shortens the production cycle, improves the utilization rate of production equipment, is suitable for most existing fermentation processes and equipment, reduces production costs, and is conducive to large-scale promotion and application.

[0048] (4) The composite microbial deodorant of the present invention is highly safe, and conditional pathogens such as Enterococcus faecalis and Pseudomonas aeruginosa are avoided when selecting strains, making it safer for the human body and the environment. BRIEF DESCRIPTION OF THE DRAWINGS

[0049] Figure 1 The removal rates of ammonia and hydrogen sulfide by different strains. The blue color represents the removal rate of ammonia, and the red color represents the removal rate of hydrogen sulfide.

[0050] Biological Deposit Description

[0051] The Latin name of Bacillus cereus GXMU-Q5.3 is Bacillus cereus GXMU-Q5.3, which is deposited in Guangdong Microbiological Culture Collection Center with the deposit number GDMCC NO: 62890, the deposit date is October 14, 2022, and the deposit address is 5th Floor, Building 59, No. 100 Xianlie Middle Road, Guangzhou;

[0052] The Latin name of Beihai Streptomyces GXMU-J15 is Streptomyces sp.GXMU-J15, which is deposited in Guangdong Microbiological Culture Collection Center with the deposit number GDMCC NO: 63901, the deposit date is October 20, 2023, and the deposit address is 5th Floor, Building 59, No. 100 Xianlie Middle Road, Guangzhou;

[0053] The Latin name of Lactobacillus paracasei GXMU-G1 is Lacticaseibacillusparacasei GXMU-G1, which is deposited in Guangdong Provincial Microbiological Culture Collection Center with the deposit number GDMCC NO: 63900. The deposit date is October 20, 2023, and the deposit address is 5th Floor, Building 59, No. 100 Xianlie Middle Road, Guangzhou. DETAILED DESCRIPTION

[0054] The invention provides a composite microbial deodorizing agent, comprising Bacillus cereus GXMU-Q5.3, Streptomyces niger GXMU-J15 and Lactobacillus paracasei GXMU-G1;

[0055] The ratio of the number of live bacteria of Bacillus cereus GXMU-Q5.3: Streptomyces niger GXMU-J15: Lactobacillus paracasei GXMU-G1 is 1-2:1-2:1-2, preferably 1:1:1;

[0056] The Latin name of the Bacillus cereus GXMU-Q5.3 is Bacillus cereus GXMU-Q5.3, which is deposited in Guangdong Microbiological Culture Collection Center with a deposit number of GDMCC NO: 62890, a deposit date of October 14, 2022, and a deposit address of 5th Floor, Building 59, No. 100 Xianlie Middle Road, Guangzhou;

[0057] The Latin name of the Beihai Streptomyces GXMU-J15 is Streptomyces sp. GXMU-J15, which is deposited in Guangdong Microbiological Culture Collection Center with the deposit number GDMCC NO: 63901, the deposit date is October 20, 2023, and the deposit address is 5th Floor, Building 59, No. 100 Xianlie Middle Road, Guangzhou;

[0058] The Latin name of the Lactobacillus paracasei GXMU-G1 is Lacticaseibacillus paracaseiGXMU-G1, which is preserved in the Guangdong Provincial Microbiological Culture Collection Center with a preservation number of GDMCCNO: 63900, a preservation date of October 20, 2023, and a preservation address of 5th Floor, Building 59, No. 100 Xianlie Middle Road, Guangzhou.

[0059] The invention provides application of the composite microbial deodorizing agent in preparing deodorizing products.

[0060] The present invention provides a liquid composite microbial deodorant, which is prepared from the composite microbial deodorant, wherein the total number of viable bacteria of Bacillus cereus GXMU-Q5.3, Streptomyces niger GXMU-J15 and Lactobacillus paracasei GXMU-G1 in the liquid composite microbial deodorant is greater than 4×10 8 CFU / mL.

[0061] The present invention provides a method for preparing the liquid composite microbial deodorant, comprising the following steps:

[0062] (1) activating Bacillus cereus GXMU-Q5.3, Streptomyces niger GXMU-J15 and Lactobacillus paracasei GXMU-G1 respectively to obtain activated Bacillus cereus GXMU-Q5.3, Streptomyces niger GXMU-J15 and Lactobacillus paracasei GXMU-G1;

[0063] (2) fermenting and culturing the activated Bacillus cereus GXMU-Q5.3, Streptomyces spp. GXMU-J15 and Lactobacillus paracasei GXMU-G1 at a temperature of 30-35° C. to obtain Bacillus cereus GXMU-Q5.3 fermentation liquid, Streptomyces spp. GXMU-J15 fermentation liquid and Lactobacillus paracasei GXMU-G1 fermentation liquid, respectively, wherein the fermentation and culturing temperature is preferably 33° C.;

[0064] (3) The fermentation liquid of Bacillus cereus GXMU-Q5.3, the fermentation liquid of Streptomyces niger GXMU-J15 and the fermentation liquid of Lactobacillus paracasei GXMU-G1 obtained in step (2) are mixed according to the ratio of viable bacteria count to obtain a liquid composite microbial deodorant.

[0065] The present invention provides a solid composite microbial deodorant, which is prepared from the composite microbial deodorant, wherein the total number of viable bacteria of Bacillus cereus GXMU-Q5.3, Streptomyces niger GXMU-J15 and Lactobacillus paracasei GXMU-G1 in the solid composite microbial deodorant is greater than 1×10 9 CFU / g.

[0066] The present invention provides a method for preparing the solid composite microbial deodorant, comprising the following steps:

[0067] (1) activating Bacillus cereus GXMU-Q5.3, Streptomyces niger GXMU-J15 and Lactobacillus paracasei GXMU-G1 respectively to obtain activated Bacillus cereus GXMU-Q5.3, Streptomyces niger GXMU-J15 and Lactobacillus paracasei GXMU-G1;

[0068] (2) fermenting and culturing the activated Bacillus cereus GXMU-Q5.3, Streptomyces spp. GXMU-J15 and Lactobacillus paracasei GXMU-G1 at a temperature of 30-35° C. to obtain Bacillus cereus GXMU-Q5.3 fermentation liquid, Streptomyces spp. GXMU-J15 fermentation liquid and Lactobacillus paracasei GXMU-G1 fermentation liquid, respectively, wherein the fermentation and culturing temperature is preferably 33° C.;

[0069] (3) collecting the Bacillus cereus GXMU-Q5.3 bacterial cells in the fermentation broth of step (2), drying, and crushing to obtain Bacillus cereus GXMU-Q5.3 bacterial powder;

[0070] (4) collecting the bacterial cells in the fermentation broth of Streptomyces beihaiensis GXMU-J15 and the fermentation broth of Lactobacillus paracasei GXMU-G1, freeze-drying and pulverizing to obtain bacterial powder of Streptomyces beihaiensis GXMU-J15 and bacterial powder of Lactobacillus paracasei GXMU-G1;

[0071] (5) The Bacillus cereus GXMU-Q5.3 powder, the North Sea Streptomyces GXMU-J15 powder and the Lactobacillus paracasei GXMU-G1 powder are mixed according to the ratio of viable bacteria count to obtain a solid composite microbial deodorant.

[0072] In the present invention, the method for collecting the fermentation broth cells in step (3) and step (4) is to centrifuge the fermentation broth at a rotation speed of 8000-12000 rpm for 4-6 minutes, preferably at a rotation speed of 10000 rpm for 5 minutes.

[0073] In the present invention, the drying temperature in step (3) is 50-70°C, preferably 60°C, and the particle size of the crushed particles is 100-300 mesh, preferably 200 mesh;

[0074] The freeze-drying temperature in step (4) is -75 to -85°C, the freeze-drying time is 5 to 7 hours, the freeze-drying temperature is preferably -80°C, the freeze-drying time is preferably 6 hours, and the crushed particle size is 100 to 300 mesh, preferably 200 mesh.

[0075] The present invention also provides the use of the liquid composite microbial deodorant or the liquid composite microbial deodorant prepared by the preparation method or the solid composite microbial deodorant or the solid composite microbial deodorant prepared by the preparation method in removing odorous substances; the odorous substances include one or more of nitrogen-containing compounds, sulfur-containing compounds, halogens and their derivatives, and oxygen-containing compounds;

[0076] The nitrogen-containing compound includes ammonia, amine or phthalamide;

[0077] The sulfur-containing compound includes hydrogen sulfide, mercaptan, sulfur dioxide or thioether;

[0078] The halogen and its derivatives include chlorine or halogenated hydrocarbons;

[0079] The oxygen-containing compound includes phenol, alcohol, organic acid or aldehyde or ketone.

[0080] The present invention also provides a method for removing odorous substances, which comprises mixing a solid composite microbial deodorizing agent or a liquid composite microbial deodorizing agent with water and spraying the mixture on a place where the odorous substances are generated;

[0081] The liquid composite microbial deodorant is the liquid composite microbial deodorant or the liquid composite microbial deodorant prepared by the preparation method;

[0082] The solid composite microbial deodorizing agent is the solid composite microbial deodorizing agent described above or the solid composite microbial deodorizing agent prepared by the preparation method described above.

[0083] In the present invention, the mass ratio of the solid composite microbial deodorant to water is 1:23-27, preferably 1:25; the mass ratio of the liquid composite microbial deodorant to water is 1:8-12, preferably 1:10;

[0084] The odorous substances include one or more of nitrogen-containing compounds, sulfur-containing compounds, halogens and their derivatives, and oxygen-containing compounds;

[0085] The nitrogen-containing compound includes ammonia, amine or phthalamide;

[0086] The sulfur-containing compound includes hydrogen sulfide, mercaptan, sulfur dioxide or thioether;

[0087] The halogen and its derivatives include chlorine or halogenated hydrocarbons;

[0088] The oxygen-containing compound includes phenol, alcohol, organic acid or aldehyde ketone;

[0089] The places where the odorous substances are produced include seafood processing waste, garbage stations, public toilets or livestock and poultry farms.

[0090] The technical solutions provided by the present invention are described in detail below in conjunction with the embodiments, but they should not be construed as limiting the protection scope of the present invention.

[0091] The preparation method of the culture medium in the embodiment of the present invention is as follows:

[0092] LB liquid medium: Mix 10.0 g of tryptone, 5.0 g of yeast extract, 10.0 g of NaCl and 1000 mL of distilled water, adjust the pH to 7.0, and sterilize at 121° C. for 20 min to obtain LB liquid medium.

[0093] LB solid medium: Mix 10.0 g of tryptone, 5.0 g of yeast extract, 10.0 g of NaCl, 20.0 g of agar and 1000 mL of distilled water, adjust the pH to 7.0, and sterilize at 121° C. for 20 min to obtain LB solid medium.

[0094] ISP2 liquid culture medium: Mix 2.0 g of yeast extract, 10.0 g of malt extract, 4.0 g of glucose and 1000 mL of distilled water, adjust the pH to 7.0, and sterilize at 121°C for 20 min to obtain ISP2 liquid culture medium.

[0095] ISP2 solid culture medium: Mix 2.0 g of yeast extract, 10.0 g of malt extract, 4.0 g of glucose, 20.0 g of agar and 1000 mL of distilled water, adjust the pH to 7.0, and sterilize at 121°C for 20 min to obtain ISP2 solid culture medium.

[0096] MRS liquid culture medium: add 10.0 g of peptone, 10.0 g of beef extract, 5.0 g of yeast extract, 2.0 g of diammonium hydrogen citrate, 20.0 g of glucose, 1.0 mL of Tween 80, 5.0 g of sodium acetate, 2.0 g of dipotassium hydrogen phosphate, 0.58 g of magnesium sulfate, and 0.25 g of manganese sulfate to 1000 mL of distilled water, adjust the pH to 7.0, and sterilize at 115°C for 30 min to obtain MRS liquid culture medium.

[0097] MRS solid culture medium: add 10.0 g of peptone, 10.0 g of beef extract, 5.0 g of yeast extract, 2.0 g of diammonium hydrogen citrate, 20.0 g of glucose, 1.0 mL of Tween 80, 5.0 g of sodium acetate, 2.0 g of dipotassium hydrogen phosphate, 0.58 g of magnesium sulfate, 0.25 g of manganese sulfate, 20.0 g of agar and 1000 mL of distilled water, adjust the pH to 7.0, and sterilize at 115°C for 30 min to obtain MRS solid culture medium.

[0098] Example 1

[0099] Screening of compound microbial deodorant strains

[0100] The invention screens out 5 strains from soil and water bodies at a dumping site of seafood processing waste, and the strains are named GXMU-Q5.3, GXMU-Q5.9, GXMU-J15, GXMU-J23 and GXMU-G1 respectively. It is found through identification that GXMU-Q5.3 is Bacillus cereus, GXMU-Q5.9 is Bacillus velez, GXMU-J15 is Streptomyces norvegicus, GXMU-J23 is Bacillus subtilis and GXMU-G1 is Lactobacillus paracasei.

[0101] Take the same volume of LB liquid medium, ISP2 liquid medium and MRS liquid medium, add ammonia water and Na2CO3 with a final concentration of 1.5% (v / v) respectively. 2 S·9H 2 O is filtered and added to S 2- A hydrogen sulfide liquid with a final concentration of 80 mg / L was prepared to obtain LB liquid culture medium, ISP2 liquid culture medium and MRS liquid culture medium containing ammonia water and hydrogen sulfide, and then the screened Bacillus cereus GXMU-Q5.3 and Bacillus Velezii GXMU-Q5.9 were inoculated in LB liquid culture medium containing ammonia water and hydrogen sulfide, respectively, and Streptomyces cerevisiae GXMU-J15 and Bacillus subtilis GXMU-J23 were inoculated in ISP2 liquid culture medium containing ammonia water and hydrogen sulfide, respectively, and Lactobacillus paracasei GXMU-G1 was inoculated in MRS liquid culture medium containing ammonia water and hydrogen sulfide. The inoculation amount of each strain was 1% (v / v). After the inoculation was completed, the culture medium was fermented at 35°C for 28 hours to obtain a fermentation broth. The LB liquid culture medium, ISP2 liquid culture medium and MRS liquid culture medium containing ammonia water and hydrogen sulfide were used as the control group, and then the release of ammonia and hydrogen sulfide in the fermentation broth of each strain and the control group was determined, and the removal rate of ammonia and hydrogen sulfide of each strain was calculated. The results are as follows: Figure 1 In the figure, the horizontal axis represents the strain, the vertical axis represents the removal rate (%), blue represents the removal rate of ammonia, red represents the removal rate of hydrogen sulfide, and in the figure, Q5.3 represents Bacillus cereus GXMU-Q5.3, Q5.9 represents Bacillus Velezii GXMU-Q5.9, J15 represents North Sea Streptomyces GXMU-J15, J23 represents Bacillus subtilis GXMU-J23, and G1 represents Lactobacillus paracasei GXMU-G1.

[0102] Ammonia was determined by Nessler's reagent spectrophotometry, and hydrogen sulfide was determined by methylene blue spectrophotometry.

[0103] The calculation formula of ammonia removal rate is: Ammonia removal rate = (ammonia release of the control group - ammonia release of the test group) / ammonia release of the control group × 100%

[0104] The calculation formula of hydrogen sulfide removal rate is: Hydrogen sulfide removal rate = (hydrogen sulfide release of control group - hydrogen sulfide release of test group) / hydrogen sulfide release of control group × 100%

[0105] according to Figure 1 It can be seen that the removal rates of ammonia and hydrogen sulfide odorous gases by Bacillus cereus GXMU-Q5.3, Streptomyces beihaiensis GXMU-J15 and Lactobacillus paracasei GXMZ-G are higher than those by Bacillus Velez GXMU-Q5.9 and Bacillus subtilis GXMU-J23. Therefore, Bacillus cereus GXMU-Q5.3, Streptomyces beihaiensis GXMU-J15 and Lactobacillus paracasei GXMZ-G were selected as strains of the composite microbial deodorant, and the three strains were preserved in the Guangdong Provincial Microbial Culture Collection Center.

[0106] Example 2

[0107] Comparison of deodorizing effects of single strain and combined strain

[0108] (1) Preparation of bacterial strains: Take one strain each of Bacillus cereus GXMU-Q5.3, Streptomyces niger GXMU-J15 and Lactobacillus paracasei GXMU-G1 preserved in Example 1;

[0109] (2) Activation of bacterial strains: Bacillus cereus GXMU-Q5.3, Streptomyces niger GXMU-J15 and Lactobacillus paracasei GXMU-G1 were inoculated into LB, ISP2 and MRS solid culture media, respectively, and cultured at 35° C. for 48 h to obtain activated Bacillus cereus GXMU-Q5.3, Streptomyces niger GXMU-J15 and Lactobacillus paracasei GXMZ-G;

[0110] (3) Expansion culture: The activated Bacillus cereus GXMU-Q5.3, Streptomyces cerevisiae GXMU-J15 and Lactobacillus paracasei GXMZ-G were inoculated into LB, ISP2 and MRS liquid culture media, respectively, and fermented at 35°C to obtain a viable cell count of 1×10 9 CFU / mL of Bacillus cereus GXMU-Q5.3 fermentation broth, Streptomyces hohmannii GXMU-J15 fermentation broth, and Lactobacillus paracasei GXMZ-G fermentation broth;

[0111] (4) Deodorization test: The test was divided into 5 groups, the first group was the control group, the second group was the fermentation liquid of Bacillus cereus GXMU-Q5.3, the third group was the fermentation liquid of Streptomyces cerae GXMU-J15, the fourth group was the fermentation liquid of Lactobacillus paracasei GXMZ-G, and the fifth group was the mixed fermentation liquid with the live bacteria count ratio of Bacillus cereus GXMU-Q5.3 fermentation liquid, Streptomyces cerae GXMU-J15 fermentation liquid and Lactobacillus paracasei GXMU-G1 fermentation liquid being 1:1:1. The test method was to take 50 mL of the fermentation liquid of groups 2 to 5 and mix it with 500 mL of tap water, and directly spray it on the organic fertilizer produced by marine processing waste. After 24 hours of spraying, the content of ammonia and hydrogen sulfide in the organic fertilizer was determined. Each group was repeated three times, and the removal rate of ammonia and hydrogen sulfide in each group was calculated, and the results were averaged. The results are shown in Table 1.

[0112] Table 1 Deodorization effect of single strain and composite strain

[0113]

[0114] According to Table 1, after the combination of Bacillus cereus GXMU-Q5.3, Streptomyces cerevisiae GXMU-J15 and Lactobacillus paracasei GXMU-G1, the ammonia removal rate reached 87.55%, which was much higher than the ammonia removal effect of the single strains of Bacillus cereus GXMU-Q5.3, Streptomyces cerevisiae GXMU-J15 and Lactobacillus paracasei GXMU-G1; the hydrogen sulfide removal effect of the composite strain was 90.32%, which was much higher than the hydrogen sulfide removal effect of the single strains of Bacillus cereus GXMU-Q5.3, Streptomyces cerevisiae GXMU-J15 and Lactobacillus paracasei GXMU-G1. This shows that the combination of the three strains will not produce an antagonistic effect, but will greatly increase the deodorization effect.

[0115] Example 3

[0116] Comparison of deodorizing effects of different strain ratios in composite microbial deodorizing agents

[0117] The number of viable bacteria obtained by culturing in Example 2 was 1×10 9CFU / mL of Bacillus cereus GXMU-Q5.3, Streptomyces cerevisiae GXMU-J15 and Lactobacillus paracasei GXMZ-G fermentation liquid were compounded according to the ratio of Bacillus cereus: Streptomyces cerevisiae: Lactobacillus paracasei live bacteria, and the live bacteria ratio of the first group was 0:1:1; the live bacteria ratio of the second group was 1:0:1; the live bacteria ratio of the third group was 1:1:0; the live bacteria ratio of the fourth group was 1:1:1; the live bacteria ratio of the fifth group was 1:2:2; the live bacteria ratio of the sixth group was 2:1:2; and the live bacteria ratio of the seventh group was 2:2:1. The test method was to take 50mL of the mixed liquid of each group and mix it with 500mL of tap water, and directly spray it on the organic fertilizer produced by marine processing waste. After 24 hours of spraying, the content of ammonia and hydrogen sulfide in the organic fertilizer was determined. Each group was repeated three times, and the removal rate of ammonia and hydrogen sulfide in each group was calculated, and the results were averaged. The results are shown in Table 2.

[0118] Table 2 Deodorization effect of different strain ratios in composite microbial deodorant

[0119] Group Proportion of viable bacteria Ammonia removal rate (%) Hydrogen sulfide removal rate (%) one 0：1：1 79.822±0.75 63.43±1.21 two 1：0：1 74.31±0.34 81.72±1.32 three 1：1：0 81.43±0.56 73.46±1.67 Four 1：1：1 88.59±0.77 90.88±1.31 five 1：2：2 83.45±0.89 86.45±1.52 six 2：1：2 84.43±0.56 79.46±1.67 seven 2：2：1 79.38±1.44 87.31±1.44

[0120] According to Table 2, microbial deodorants with different proportions have a certain deodorizing effect on ammonia and hydrogen sulfide, but the proportion of microbial strains is the key to improving the effect of deodorants. Only by adjusting to the optimal configuration ratio and forming a stable microecological flora with synergistic effects can the best deodorizing effect be achieved. The deodorizing effect of three strains is better than that of two strains. When the ratio of the live bacteria count of Bacillus cereus GXMU-Q5.3, Streptomyces cerevisiae GXMU-J15 and Lactobacillus paracasei GXMU-G1 is 1:1:1, the removal effect of ammonia and hydrogen sulfide is the best, with ammonia removal rate of 88.59% and hydrogen sulfide removal rate of 90.88%.

[0121] Example 4

[0122] Preparation of a liquid composite microbial deodorant

[0123] The Bacillus cereus GXMU-Q5.3, Streptomyces cerevisiae GXMU-J15 and Lactobacillus paracasei GXMU-G1 preserved in Example 1 were inoculated into LB, ISP2 and MRS solid culture media, respectively, and activated for 48 h at 30 ° C to obtain activated Bacillus cereus GXMU-Q5.3, Streptomyces cerevisiae GXMU-J15 and Lactobacillus paracasei GXMU-G1; the activated Bacillus cereus GXMU-Q5.3, Streptomyces cerevisiae GXMU-J15 and Lactobacillus paracasei GXMU-G1 were inoculated into LB, ISP2 and MRS liquid culture media, respectively, and fermented and cultured at 33 ° C to obtain viable bacteria with a count of 1×10 9CFU / mL of Bacillus cereus GXMU-Q5.3 fermentation broth, Streptomyces cereus GXMU-J15 fermentation broth and Lactobacillus paracasei GXMU-G1 fermentation broth; the fermentation broths were mixed in a ratio of 1:1:1 to obtain a total viable count of 1×10 9 CFU / mL of liquid composite microbial deodorant.

[0124] Example 5

[0125] Preparation of a liquid composite microbial deodorant

[0126] The Bacillus cereus GXMU-Q5.3, Streptomyces cerevisiae GXMU-J15 and Lactobacillus paracasei GXMU-G1 preserved in Example 1 were inoculated into LB, ISP2 and MRS solid culture media, respectively, and activated at 37°C for 40h to obtain activated Bacillus cereus GXMU-Q5.3, Streptomyces cerevisiae GXMU-J15 and Lactobacillus paracasei GXMU-G1; the activated Bacillus cereus GXMU-Q5.3, Streptomyces cerevisiae GXMU-J15 and Lactobacillus paracasei GXMU-G1 were inoculated into LB, ISP2 and MRS liquid culture media, respectively, and fermented and cultured at 33°C to obtain viable bacteria with a count of 1×10 9 CFU / mL of Bacillus cereus GXMU-Q5.3 fermentation broth, Streptomyces cerevisiae GXMU-J15 fermentation broth and Lactobacillus paracasei GXMU-G1 fermentation broth; the fermentation broth was mixed in a ratio of 1:2:2 of Bacillus cereus GXMU-Q5.3: Streptomyces cerevisiae GXMU-J15: Lactobacillus paracasei GXMU-G1 to obtain a total viable count of 1×10 9 CFU / mL of liquid composite microbial deodorant.

[0127] Example 6

[0128] Preparation of a solid composite microbial deodorant

[0129] The Bacillus cereus GXMU-Q5.3, Streptomyces cerevisiae GXMU-J15 and Lactobacillus paracasei GXMU-G1 preserved in Example 1 were inoculated into LB, ISP2 and MRS solid culture media, respectively, and activated for 48 h at 32 ° C to obtain activated Bacillus cereus GXMU-Q5.3, Streptomyces cerevisiae GXMU-J15 and Lactobacillus paracasei GXMU-G1; the activated Bacillus cereus GXMU-Q5.3, Streptomyces cerevisiae GXMU-J15 and Lactobacillus paracasei GXMU-G1 were inoculated into LB, ISP2 and MRS liquid culture media, respectively, and fermented and cultured at 33 ° C to obtain viable bacteria with a count of 1 × 10 9CFU / mL of Bacillus cereus GXMU-Q5.3 fermentation broth, Streptomyces beijingensis GXMU-J15 fermentation broth and Lactobacillus paracasei GXMU-G1 fermentation broth; the Bacillus cereus GXMU-Q5.3 fermentation broth was centrifuged at a speed of 10000 rpm for 5 min, the cells were collected, dried at 50°C to a moisture content of 35% (w / w), and then crushed to a particle size of 200 mesh to obtain a viable cell count of 2×10 9 CFU / g of Bacillus cereus GXMU-Q5.3 powder; the fermentation broth of Streptomyces cereus GXMU-J15 and the fermentation broth of Lactobacillus paracasei GXMU-G1 were centrifuged at 10000 rpm for 5 min, the cells were collected, and then freeze-dried at -80 ° C for 6 h, and then crushed to a particle size of 200 mesh, and the viable counts were 2×10 9 CFU / g of Streptomyces GXMU-J15 powder and Lactobacillus paracasei GXMU-G1 powder; Bacillus cereus GXMU-Q5.3 powder: Streptomyces GXMU-J15 powder: Lactobacillus paracasei GXMU-G1 powder were mixed at a live bacterial count ratio of 1:1:1 to obtain a total live bacterial count of 2×10 9 CFU / mL solid composite microbial deodorant.

[0130] Example 7

[0131] Preparation of a solid composite microbial deodorant

[0132] The Bacillus cereus GXMU-Q5.3, Streptomyces cerevisiae GXMU-J15 and Lactobacillus paracasei GXMU-G1 preserved in Example 1 were inoculated into LB, ISP2 and MRS solid culture media, respectively, and activated for 40 h at 35 ° C to obtain activated Bacillus cereus GXMU-Q5.3, Streptomyces cerevisiae GXMU-J15 and Lactobacillus paracasei GXMU-G1; the activated Bacillus cereus GXMU-Q5.3, Streptomyces cerevisiae GXMU-J15 and Lactobacillus paracasei GXMU-G1 were inoculated into LB, ISP2 and MRS liquid culture media, respectively, and fermented and cultured at 33 ° C to obtain viable bacteria with a count of 1×10 9 CFU / mL of Bacillus cereus GXMU-Q5.3 fermentation broth, Streptomyces beijingensis GXMU-J15 fermentation broth and Lactobacillus paracasei GXMU-G1 fermentation broth; the Bacillus cereus GXMU-Q5.3 fermentation broth was centrifuged at a speed of 10000 rpm for 5 min, the cells were collected, dried at 50°C to a moisture content of 35% (w / w), and then crushed to a particle size of 200 mesh to obtain a viable cell count of 2×10 9CFU / g of Bacillus cereus GXMU-Q5.3 powder; the fermentation broth of Streptomyces cereus GXMU-J15 and the fermentation broth of Lactobacillus paracasei GXMU-G1 were centrifuged at 10000 rpm for 5 min, the cells were collected, and then freeze-dried at -80 ° C for 6 h, and then crushed to a particle size of 200 mesh, and the viable counts were 2×10 9 CFU / g of Streptomyces GXMU-J15 powder and Lactobacillus paracasei GXMU-G1 powder; Bacillus cereus GXMU-Q5.3 powder: Streptomyces GXMU-J15 powder: Lactobacillus paracasei GXMU-G1 powder were mixed in a ratio of 1:2:2 to obtain a total live bacterial count of 2×10 9 CFU / g solid composite microbial deodorant.

[0133] Example 8

[0134] Preparation of a solid composite microbial deodorant

[0135] The Bacillus cereus GXMU-Q5.3, Streptomyces cerevisiae GXMU-J15 and Lactobacillus paracasei GXMU-G1 preserved in Example 1 were inoculated into LB, ISP2 and MRS solid culture media, respectively, and activated at 30°C for 50h to obtain activated Bacillus cereus GXMU-Q5.3, Streptomyces cerevisiae GXMU-J15 and Lactobacillus paracasei GXMU-G1; the activated Bacillus cereus GXMU-Q5.3, Streptomyces cerevisiae GXMU-J15 and Lactobacillus paracasei GXMU-G1 were inoculated into LB, ISP2 and MRS liquid culture media, respectively, and fermented and cultured at 33°C to obtain viable bacteria with a count of 1×10 9 CFU / mL of Bacillus cereus GXMU-Q5.3 fermentation broth, Streptomyces beijingensis GXMU-J15 fermentation broth and Lactobacillus paracasei GXMU-G1 fermentation broth; the Bacillus cereus GXMU-Q5.3 fermentation broth was centrifuged at a speed of 10000 rpm for 5 min, the cells were collected, dried at 50°C to a moisture content of 35% (w / w), and then crushed to a particle size of 200 mesh to obtain a viable cell count of 2×10 9 CFU / g of Bacillus cereus GXMU-Q5.3 powder; the fermentation broth of Streptomyces cereus GXMU-J15 and the fermentation broth of Lactobacillus paracasei GXMU-G1 were centrifuged at 10000 rpm for 5 min, the cells were collected, and then freeze-dried at -80 ° C for 6 h, and then crushed to a particle size of 200 mesh, and the viable counts were 2×10 9CFU / g of Streptomyces GXMU-J15 powder and Lactobacillus paracasei GXMU-G1 powder; Bacillus cereus GXMU-Q5.3 powder: Streptomyces GXMU-J15 powder: Lactobacillus paracasei GXMU-G1 powder were mixed in a ratio of 2:1:2 to obtain a total live bacterial count of 2×10 9 CFU / g solid composite microbial deodorant.

[0136] Example 9

[0137] Deodorizing effect of liquid composite microbial deodorizing agent

[0138] Take 10g of the liquid composite microbial deodorant prepared in Example 4 and add 100g, 500g and 1000g of tap water to mix, respectively, to obtain bacterial solutions with water mass ratios of 1:10, 1:50 and 1:100, respectively. Spray the bacterial solutions with different mass ratios directly on the organic fertilizer produced by livestock and poultry manure, and repeat three times for each mass ratio of bacterial solution. After 24h of spraying, determine the content of ammonia and hydrogen sulfide in the organic fertilizer to calculate the removal rate and take the average value. The results are shown in Table 3.

[0139] Table 3 Deodorization effect of liquid composite microbial deodorant with different water addition ratios

[0140]

[0141]

[0142] According to Table 3, when the mass ratio of liquid composite microbial deodorant to water is 1:10, the removal rates of ammonia and hydrogen sulfide are the highest, which are 85.82% and 83.43% respectively.

[0143] Example 10

[0144] Deodorizing effect of solid composite microbial deodorizing agent

[0145] Take 10g of the solid composite microbial deodorant prepared in Example 6 and add 250g, 500g and 1000g of tap water to mix, respectively, to obtain bacterial solutions with water mass ratios of 1:25, 1:50 and 1:100, respectively. Spray the bacterial solutions with different mass ratios directly on the organic fertilizer produced by livestock and poultry manure, and repeat three times for each mass ratio of bacterial solution. After 24h of spraying, determine the content of ammonia and hydrogen sulfide in the organic fertilizer to calculate the removal rate and take the average value. The results are shown in Table 4.

[0146] Table 4 Deodorization effect of solid composite microbial deodorant with different water addition ratios

[0147] Water mass ratio Ammonia removal rate (%) Hydrogen sulfide removal rate (%) 1：25 86.34±0.36 84.56±1.42 1：50 75.35±0.42 82.44±1.05 1：100 72.56±0.80 73.41±1.22

[0148] According to Table 4, when the mass ratio of liquid composite microbial deodorant to water is 1:25, the removal rates of ammonia and hydrogen sulfide are the highest, which are 86.34% and 84.56% respectively.

[0149] As can be seen from the above embodiments, the present invention provides a composite microbial deodorizing agent. The strains of the composite microbial deodorizing agent of the present invention are screened from the soil and water bodies at the dumping site of seafood processing waste, and have obvious effects on decomposing and suppressing odor of seafood processing waste. The bacteria have a synergistic effect, and the composite microbial agent has a deodorizing effect of more than 40% higher than that of a single strain, and has low cost, high efficiency, wide adaptability, short deodorizing cycle, and high safety.

[0150] The above is only a preferred embodiment of the present invention. It should be pointed out that for ordinary technicians in this technical field, several improvements and modifications can be made without departing from the principle of the present invention. These improvements and modifications should also be regarded as the scope of protection of the present invention.

Claims

1. A composite microbial deodorant, characterized in that: These include Bacillus cereus GXMU-Q5.3, Streptomyces norvegicus GXMU-J15, and Lactobacillus paracasei GXMU-G1; The ratio of the number of live bacteria of Bacillus cereus GXMU-Q5.3: Streptomyces cerevisiae GXMU-J15: Lactobacillus paracasei GXMU-G1 is 1-2:1-2:1-2; The Latin name of the Bacillus cereus GXMU-Q5.3 is Bacillus cereus GXMU-Q5.3, which is deposited in Guangdong Microbiological Culture Collection Center with a deposit number of GDMCC NO: 62890, a deposit date of October 14, 2022, and a deposit address of 5th Floor, Building 59, No. 100 Xianlie Middle Road, Guangzhou; The Latin name of the Beihai Streptomyces GXMU-J15 is Streptomyces sp. GXMU-J15, which is deposited in Guangdong Microbiological Culture Collection Center with the deposit number GDMCC NO: 63901, the deposit date is October 20, 2023, and the deposit address is 5th Floor, Building 59, No. 100 Xianlie Middle Road, Guangzhou; The Latin name of the Lactobacillus paracasei GXMU-G1 is Lacticaseibacillus paracasei GXMU-G1, which is preserved in the Guangdong Provincial Microbiological Culture Collection Center with a preservation number of GDMCCNO: 63900, a preservation date of October 20, 2023, and a preservation address of 5th Floor, Building 59, No. 100 Xianlie Middle Road, Guangzhou.

2. Use of the composite microbial deodorant agent according to claim 1 in the preparation of deodorant products.

3. A liquid composite microbial deodorant, characterized in that: The liquid composite microbial deodorant is prepared from the composite microbial deodorant according to claim 1, wherein the total number of viable bacteria of Bacillus cereus GXMU-Q5.3, Streptomyces niger GXMU-J15 and Lactobacillus paracasei GXMU-G1 in the liquid composite microbial deodorant is greater than 4×10 8 CFU / mL.

4. The method for preparing the liquid composite microbial deodorant according to claim 3, characterized in that: The steps include: (1) activating Bacillus cereus GXMU-Q5.3, Streptomyces niger GXMU-J15 and Lactobacillus paracasei GXMU-G1 respectively to obtain activated Bacillus cereus GXMU-Q5.3, Streptomyces niger GXMU-J15 and Lactobacillus paracasei GXMU-G1; (2) fermenting and culturing the activated Bacillus cereus GXMU-Q5.3, Streptomyces cerevisiae GXMU-J15 and Lactobacillus paracasei GXMU-G1 at a temperature of 30 to 35° C. to obtain Bacillus cereus GXMU-Q5.3 fermentation liquid, Streptomyces cerevisiae GXMU-J15 fermentation liquid and Lactobacillus paracasei GXMU-G1 fermentation liquid; (3) The fermentation liquid of Bacillus cereus GXMU-Q5.3, the fermentation liquid of Streptomyces niger GXMU-J15 and the fermentation liquid of Lactobacillus paracasei GXMU-G1 obtained in step (2) are mixed according to the ratio of viable bacteria count to obtain a liquid composite microbial deodorant.

5. A solid composite microbial deodorant, characterized in that: The solid composite microbial deodorant is prepared from the composite microbial deodorant according to claim 1, wherein the total number of viable bacteria of Bacillus cereus GXMU-Q5.3, Streptomyces niger GXMU-J15 and Lactobacillus paracasei GXMU-G1 in the solid composite microbial deodorant is greater than 1×10 9 CFU / g.

6. The method for preparing the solid composite microbial deodorant according to claim 5, characterized in that: The steps include: (1) activating Bacillus cereus GXMU-Q5.3, Streptomyces niger GXMU-J15 and Lactobacillus paracasei GXMU-G1 respectively to obtain activated Bacillus cereus GXMU-Q5.3, Streptomyces niger GXMU-J15 and Lactobacillus paracasei GXMU-G1; (2) fermenting and culturing the activated Bacillus cereus GXMU-Q5.3, Streptomyces spp. GXMU-J15 and Lactobacillus paracasei GXMU-G1 at a temperature of 30-35° C. to obtain Bacillus cereus GXMU-Q5.3 fermentation liquid, Streptomyces spp. GXMU-J15 fermentation liquid and Lactobacillus paracasei GXMU-G1 fermentation liquid; (3) collecting the Bacillus cereus GXMU-Q5.3 bacterial cells in the fermentation broth of step (2), drying, and crushing to obtain Bacillus cereus GXMU-Q5.3 bacterial powder; (4) collecting the bacterial cells in the fermentation broth of Streptomyces beihaiensis GXMU-J15 and the fermentation broth of Lactobacillus paracasei GXMU-G1, freeze-drying and pulverizing to obtain bacterial powder of Streptomyces beihaiensis GXMU-J15 and bacterial powder of Lactobacillus paracasei GXMU-G1; (5) The Bacillus cereus GXMU-Q5.3 powder, the North Sea Streptomyces GXMU-J15 powder and the Lactobacillus paracasei GXMU-G1 powder are mixed according to the ratio of viable bacteria count to obtain a solid composite microbial deodorant.

7. The preparation method according to claim 6, characterized in that: The drying temperature in step (3) is 50-70° C., and the particle size of the crushed particles is 100-300 meshes; The freeze-drying temperature in step (4) is -75 to -85°C, the freeze-drying time is 5 to 7 hours, and the particle size of the crushed product is 100 to 300 meshes.

8. Use of the liquid composite microbial deodorant according to claim 3 or the liquid composite microbial deodorant prepared by the preparation method according to claim 4 or the solid composite microbial deodorant according to claim 5 or the solid composite microbial deodorant prepared by the preparation method according to any one of claims 6 to 7 in removing odorous substances; the odorous substances include one or more of nitrogen-containing compounds, sulfur-containing compounds, halogens and their derivatives, and oxygen-containing compounds; The nitrogen-containing compound includes ammonia, amine or phthalamide; The sulfur-containing compound includes hydrogen sulfide, mercaptan, sulfur dioxide or thioether; The halogen and its derivatives include chlorine or halogenated hydrocarbons; The oxygen-containing compound includes phenol, alcohol, organic acid or aldehyde or ketone.

9. A method for removing odorous substances, characterized in that: Mix the solid composite microbial deodorant or the liquid composite microbial deodorant with water and spray it on the place where the odorous substances are produced; The liquid composite microbial deodorant is the liquid composite microbial deodorant according to claim 3 or the liquid composite microbial deodorant prepared by the preparation method according to claim 4; The solid composite microbial deodorant is the solid composite microbial deodorant according to claim 5 or the solid composite microbial deodorant prepared by the preparation method according to any one of claims 6 to 7.

10. The method according to claim 9, characterized in that The mass ratio of the solid composite microbial deodorant to water is 1:23-27; the mass ratio of the liquid composite microbial deodorant to water is 1:8-12. The odorous substances include one or more of nitrogen-containing compounds, sulfur-containing compounds, halogens and their derivatives, and oxygen-containing compounds; The nitrogen-containing compound includes ammonia, amine or phthalamide; The sulfur-containing compound includes hydrogen sulfide, mercaptan, sulfur dioxide or thioether; The halogen and its derivatives include chlorine or halogenated hydrocarbons; The oxygen-containing compound includes phenol, alcohol, organic acid or aldehyde ketone; The places where the odorous substances are produced include seafood processing waste, garbage stations, public toilets or livestock and poultry farms.

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