The invention provides a preparation method of a novel chiral chromatographic column stationary phase based on phenylalanine. The preparation method comprises the following steps: 1, carrying out surface treatment on a silica gel sphere; 2, preparing a phenylalanine chiral selector monomer; and 3, preparing the chiral chromatographic column stationary phase. The method has the advantages of simple process route, low cost and operation convenience, and the prepared chiral chromatographic column stationary phase has the advantages of wide chiral selectivity, wide mobile phase application range, good bonding force, difficult loss, long service life, short separation time, no tailing, high column efficiency and the like.