14 results about "Esterase Gene" patented technology

The invention discloses SGNH family esterase SH2 screened in a Tibetan hot spring environment and application of the SGNH family esterase SH2, and belongs to the technical field of gene engineering. According to the invention, a new SGNH family esterase gene is screened from a Tibetan hot spring environment, and recombinant expression is realized in escherichia coli. The recombinant esterase prepared by the invention has the characteristics of thermal stability, salt resistance, organic solvent resistance and the like, can be safely applied to tooth whitening or skin exfoliating products, avoids damage of traditional chemical bleaching agents to enamel, and has important application value in the fields of daily chemicals, medicines, food processing and the like.

The invention discloses an application of an overexpression level transfer gene GlpQ in improving the fertility of male silkworms, and the nucleotide sequence of the GlpQ is shown as SEQ ID NO.3. After the GlpQ1 gene is overexpressed, the reproductive activity of the male silkworms is obviously enhanced, the number of female silkworms capable of mating with each male silkworm on average is obviously increased, and the reproductive activity of the male silkworms is obviously improved. Correspondingly, the number of female silkworms capable of being fertilized by the male silkworms is also obviously increased, the testis development process can be promoted, the gene may act on the testis outer membrane to promote the increase of the testis volume and act on the seminal vesicle to promote sperm formation, and therefore the gene can be used for the fertility of the male silkworms.

This invention discloses the SGNH family esterase SH2 screened from the hot spring environment of Tibet and its applications, belonging to the field of genetic engineering technology. This invention screened a novel SGNH family esterase gene from the hot spring environment of Tibet and achieved recombinant expression in *E. coli*. The recombinant esterase prepared by this invention is thermally stable, salt-resistant, and resistant to organic solvents, and can be safely used in teeth whitening or skin exfoliation products, avoiding damage to tooth enamel caused by traditional chemical bleaching agents. It has significant application value in the fields of daily chemicals, pharmaceuticals, and food processing.

Recombinant cells and methods for producing methyl ketones, such as medium-chain methyl ketones. The recombinant cells include recombinant acyl-ACP thioesterase genes, recombinant β-ketoacyl-CoA thioesterase genes, and recombinant acyl-CoA synthetase genes, in addition to other modifications. The methods include culturing the recombinant cells to produce the methyl ketones and isolating the produced methyl ketones.

The invention discloses an engineering strain for producing urolithin A and application of the engineering strain. The engineering strain contains a tannin acyl hydrolase gene tah, an esterase gene lip, a gallic acid decarboxylase gene gdo and a dopamine dehydroxylase gene ascd. The four artificially synthesized genes are jointly constructed on an escherichia coli expression vector, so that a brand-new vector containing four co-expression genes is constructed, then the brand-new vector is transferred into a chassis strain cell to realize co-expression of the four genes, and four enzymes expressed in the cell all have activity; and the obtained heavy engineering strain can stably convert the extracellular added ellagic tannin into the urolithin A, so that the whole-cell catalysis process of producing the urolithin A by taking the ellagic tannin as a substrate is realized.

The invention belongs to the field of biotechnology and biocatalysis, and discloses application of ester hydrolase in preparation of beta-keto acid compounds. A new esterase gene is subjected to heterologous overexpression in escherichia coli through a genetic engineering means to obtain a genetically engineered bacterium for efficiently expressing esterase, and the genetically engineered bacterium is obtained by taking escherichia coli as a host and transferring a recombinant plasmid carrying an esterase gene. The engineering bacterium can efficiently express and produce ester hydrolase, has the advantages of strong substrate tolerance, high concentration feeding, high catalytic efficiency, conversion rate of 99% or more, no by-product generation, short reaction period, less biocatalyst dosage, simple and convenient preparation method, mild conditions and environmental friendliness, and has good industrialization prospects.

The invention discloses a recombinant esterase, an engineering bacterium and application of the recombinant esterase in hydrolysis of spirotetramat. The esterase gene provided by the invention can be cloned and expressed in escherichia coli. The recombinant esterase CXest is used for catalytic hydrolysis of spirotetramat, the substrate concentration is 200 mg / L, the catalysis time is 1 h, the conversion rate is 74.6%, and the main product is spirotetramat.

The application discloses application of a broad-spectrum type III esterase derived from Rhodococcus in phthalate ester pollution bioremediation, and overcomes the limitation of low degradation efficiency of a natural strain; through genetic engineering technology, the esterase 5359 gene is cloned into a Rhodococcus-E.coli shuttle vector pNV18 to construct a recombinant Rhodococcus engineering bacterium WT-pNV18-5359 overexpressing the esterase. Experimental results show that the engineering bacterium significantly improves the degradation performance: in single PAEs degradation test, the degradation rates of long-chain PAEs such as DEHP, DnOP and DiNP are respectively increased by 15.71%, 13.99% and 12.82% compared with the wild type; in a mixed PAEs system, the degradation of long-chain PAEs is obviously superior, and the engineering bacterium has good genetic stability and does not affect growth. Through genetic engineering to strengthen the application of known enzymes, efficient and stable PAEs pollution bioremediation is realized.

The present application belongs to the technical field of plant genetic engineering, and particularly relates to a moso bamboo pectin acetyl esterase gene PePAE6 as well as an extraction method and application thereof. The nucleotide sequence of the gene is shown as SEQ ID NO:1. The gene has the functions of reducing the pectin acetylation level of plant cell walls, promoting pectin accumulation in plants, improving the photosynthetic capacity of plants, and promoting the growth of plants. The moso bamboo pectin acetyl esterase gene PePAE6 is introduced into rice for verification, and the transgenic rice plants have a lower pectin acetylation level of cell walls, a higher pectin accumulation amount, a stronger photosynthetic capacity of leaves, and a faster and better growth than wild-type rice plants.

When employed, a recombinant expression vector carrying a daptomycin production gene including a foreign fatty acyl AMP ligase (FAAL) gene, a thioesterase (TE) gene, and a fatty acid synthase (FAS) gene according to the present invention can enhance specificity for decanoic acid, and produce daptomycin without supplementation of lipopeptides, such as decanoic acid, from the outside of the strain. In addition, no by-products are generated during the production process, thereby enabling production of daptomycin with high purity and high yield.

The tea geometrid antennal esterase EoblCXE27 gene and the interfering RNA of the gene and the application thereof belong to the technical field of biotechnology.The application provides the application of the tea geometrid antennal esterase EoblCXE27 gene and the application of the interfering RNA.The results show that the tea geometrid antennal esterase EoblCXE27 gene can be successfully interfered after the siEoblCXE27 is injected into the larvae and female adults.Compared with the control group, the sensitivity of the tea geometrid larvae after the antennal esterase EoblCXE27 gene is interfered to bifenthrin is significantly increased.The application will help to develop the green control technology of the tea geometrid with the antennal esterase EoblCXE27 gene as a target.

The invention provides a method for preparing cholinesterase through an escherichia coli expression system, which comprises the following steps: synthesizing a cholinesterase gene, amplifying the synthesized cholinesterase gene, carrying out electrophoresis on a target fragment of the cholinesterase gene, and adding a linearized vector pET28a and the target fragment of the cholinesterase gene subjected to double enzyme digestion into an escherichia coli expression system, the preparation method comprises the following steps of: adding a recombinant plasmid pET28a-chE into a T4 ligase reaction system, incubating and connecting to construct a recombinant plasmid pET28a-chE, converting the recombinant plasmid pET28a-chE into E. coli BL21 (DE3) escherichia coli competent cells, and culturing; and the recombinant strain E.coli BL21 (DE3) / pET28a-chE is obtained. According to the invention, high-efficiency expression of cholinesterase in escherichia coli is successfully realized, the problems of long cell culture period, high culture medium cost, difficulty in process amplification and the like due to dependence of mammalian cell expression systems in the existing cholinesterase preparation process can be solved, and the obtained enzyme amount can reach 1mg / 1g thalli.

The invention relates to the technical field of genetic engineering, in particular to bemisia tabaci pectin methylesterase, a BtPME1 gene, dsRNA and application of the bemisia tabaci pectin methylesterase to prevention and treatment of bemisia tabaci. The amino acid sequence of the bemisia tabaci pectin methylesterase provided by the invention is as shown in SEQ ID NO. 2. Based on bemisia tabaci genome data in the early stage of a laboratory, a bemisia tabaci horizontal transfer gene is analyzed, it is found that a plant-derived horizontally-transferred pectin-like methylesterase coding gene BtPME1 exists in bemisia tabaci, and the amino acid sequence of pectin-like methylesterase expressed by the coding gene BtPME1 is shown as SEQ ID NO.2. After the BtPME1 gene is silenced, the survival rate and egg laying amount of bemisia tabaci on a host plant can be effectively reduced. The invention provides a new target for a bemisia tabaci green control technology based on RNA interference guidance, and opens up a new way for improving the insect resistance of crops.