The invention discloses thermophilic
esterase derived from an aquifex aeolicus strain and
functional verification of the thermophilic
esterase, and belongs to the field of bioengineering technology. According to the invention, a novel thermophilic
esterase gene is discovered, three expression systems are constructed, and efficient expression and research of enzymatic properties are achieved. Construction of an eukaryotic expression
system: preferably, a vector pPIC9K is adopted for
expression vector construction, and a
pichia pastoris host, preferably GS115, is transformed, so that efficient expression is achieved; construction of a prokaryotic
escherichia coli expression
system: preferably, a vector MBP3 is adopted for
expression vector construction, and
escherichia coli hosts, preferably BL21 and Origami2, are transformed, so that efficient expression is achieved; and construction of a prokaryotic
bacillus megaterium expression
system: preferably, a vector pHIS1525 is adopted for
expression vector construction, and a
bacillus megaterium host, preferably YYBm1, is transformed, so that efficient expression is achieved. The
recombinant enzyme (the thermophilic esterase) has the advantages of esterase activity, ,thermophilic characteristic,
thermal stability and the like; and the
recombinant enzyme has a great potential in industrial application under a high-temperature condition.