6 results about "Genetically modified organism" patented technology

This invention relates to the field of genetic engineering. Specifically, it relates to cytosine deaminases and their use in base editing. More specifically, it relates to a method for screening and identifying deaminases, a base editing system based on newly identified cytosine deaminases, a method for base editing a target sequence in the genome of an organism (e.g., a plant) using this base editing system, and genetically modified organisms (e.g., plants) and their offspring produced by said method.

A biosynthetic method and composition for producing indigo dyes comprising indigotin and isatin. A tryptophanase (TRP) and a flavin monooxygenase (FMO) can be produced through a transgenic organism having carrier encoded tryptophanase and flavin monooxygenase. The produced tryptophanase and flavin monooxygenase convert L-tryptophan into indigo dyes in the presence of one or more bioactive additives selected from cysteine, 2-hydroxyindole, or 2-indoxyl, and the weight ratio of indigotin to isatin in the dyes is between 1:0.1 to 1:4. The dyes have a hue of X: 0.1920 to 0.2481, Y: 0.2537 to 0.2019, Z: 0.5543 to 0.5501 in the CIE XYZ color space definition. Selectable and repeatable dyeing hues are provided.

This invention provides enzymes that promote the stable and / or intracellularly abundant synthesis of luciferin from precursor compounds, and the reduction of oxyluciferin to luciferin, as well as uses thereof. [Solution] A method for preparing compounds identical to fungal luciferin and preluciferin in vitro or in vivo is provided; an expression cassette comprising nucleic acids, vectors, and regulatory elements necessary for nucleic acid expression in selected host cells; further, a combination of nucleic acids for obtaining cells, stable cell lines, transgenic organisms (e.g., plants, animals, fungi, or microorganisms), autoluminescent cells, cell lines, or transgenic organisms containing nucleic acids, vectors, or expression cassettes; a combination of proteins for producing luciferin or its precursors from simpler compounds; and a kit comprising nucleic acids, vectors, or expression cassettes for producing luminescent cells, cell lines, or transgenic organisms.

Provided is a computer implemented method for mapping transgene integration into n organism by providing a custom genome comprising a reference genome sequence and a transgene sequence, aligning sequence reads of the transgenic organism to the custom genome, identifying candidate junction reads, wherein the candidate junction reads correspond to sequence reads comprising sequence regions that align to the transgene sequence and to the host sequence, categorizing the candidate junction reads into junction clusters, wherein each of the junction clusters comprises a plurality of the candidate junction reads each with an alignment segment terminus within a cluster proximity region of the custom genome, determining a transgene junction based on the junction clusters that possesses at least three of the candidate junction reads within the cluster proximity region of the custom genome and mapping the integration of the transgene into the transgenic organism based on the transgene junctions.

  42      Abstract  The present invention is directed to nucleic acid molecules which encode novel luciferases, functional fragments thereof, homologs and mutants, as well as to proteins encoded by said nucleic acids. The nucleic acid molecules of interest are isolated from fungi or obtained by genetic engineering methods. Also, host cells, stable cell lines and transgenic organisms comprising said nucleic acid molecules are provided. In addition, antibodies specific to the proteins of the present invention are provided. Said proteins and nucleic acids are used in many applications and methods, in particular, in labelling organisms, cells, cellular organelles, or proteins. Also, said protein and nucleotide compositions are used in methods for detecting protein-protein interactions, for testing promoter activity under various conditions. Finally, provided are kits for the use of proteins and nucleic acids of the present invention in the diversity of methods and applications. Abstract The present invention is directed to nucleic acid molecules which encode novel luciferases, functional fragments thereof, homologs and mutants, as well as to proteins encoded by said nucleic acids. The nucleic acid molecules of interest are isolated from fungi or obtained by genetic engineering methods. Also, host cells, stable cell lines and transgenic organisms comprising said nucleic acid molecules are provided. In addition, antibodies specific to the proteins of the present invention are provided. Said proteins and nucleic acids are used in many applications and methods, in particular, in labelling organisms, cells, cellular organelles, or proteins. Also, said protein and nucleotide compositions are used in methods for detecting protein-protein interactions, for testing promoter activity under various conditions. Finally, provided are kits for the use of proteins and nucleic acids of the present invention in the diversity of methods and applications. 42 20 24 20 33 78 21 M ay 2 02 4 A b s t r a c t 2 0 2 4 2 0 3 3 7 8 2 1 M a y 2 0 2 4

This invention provides a strain of Akkermansia myxophilus ( Akkermansia muciniphila The present invention provides a probiotic preparation comprising the aforementioned Akkermansia myxophilus or its post-genetically modified organism (PGA). The Akkermansia myxophilus or its post-genetically modified organism (PGA) of the present invention can improve vascular endothelial cell function, increase testosterone secretion, and thus improve erectile dysfunction and reproductive capacity. The probiotic preparation of the present invention may comprise the aforementioned Akkermansia myxophilus or its post-genetically modified organism (PGA) and yohimbine. This preparation can improve erectile dysfunction caused by vascular endothelial dysfunction, improve reproductive function in aged mice, and the components in this preparation have a synergistic effect.