55 results about "Genetically modified organism" patented technology

The present invention relates to the field of genetic engineering. Specifically, the present invention relates to cytosine deaminase and use thereof in base editing. More specifically, the present invention relates to a base editing system based on a newly identified cytosine deaminase, a method for base editing a target sequence in the genome of an organism (e.g., a plant) using the base editing system, and a genetically modified organism (e.g., a plant) produced by the method and progenies thereof.

The invention relates to the field of gene engineering. In particular, the present invention relates to cytosine deaminases and their use in base editing. More specifically, the invention relates to a method for screening and identifying a deaminase, a base editing system based on a newly identified cytosine deaminase, a method for editing a target sequence in a genome of an organism (such as a plant) by using the base editing system, and a method for screening and identifying the target sequence. As well as genetically modified organisms (e.g., plants) and progeny thereof produced by the method.

The invention discloses application of SmCLE mature peptide in inhibiting plant aging, and belongs to the technical field of plant biology. The invention discloses application of a SmCLE mature peptide in inhibiting plant aging. The amino acid sequence of the SmCLE mature peptide is SEQ ID NO: 1. The CLE peptide is applied to the aspect of inhibiting plant aging, solves the problem that the existing CLE peptide cannot be applied to anti-aging of plants, especially medicinal plants or non-mode crops, has the advantages of small molecular weight, easiness in synthesis, low action concentration and good biocompatibility, can realize accurate regulation and control of leaf aging through exogenous application, and has a wide application prospect. And the safety problem of transgenic organisms is avoided.

The present invention relates to the field of genome-editing, more specific of difficult to transform organisms such as e.g. invertebrates and in particular Arthropoda. The present disclosure provides a combination comprising one or more cationic nanoparticles, one or more endosomal escape reagent(s), and one or more ribonucleoprotein (RNP) complexes wherein said RNP comprises a Cas RNA-guided nuclease associated with a guide RNA (gRNA), and the use thereof in the production of a non-human genetically modified organism. The invention further provides a combination as defined herein for use in CRISPR based methods.

This invention provides the nucleic acid molecules encoding novel small monomeric near-infrared fluorescent protein miRFP670nano, variants and derivatives thereof as well as proteins and peptides encoded by these nucleic acids. The invention also relates to derivatives, homologues, or mutants of the specific proteins referenced above as well as fragments of the nucleic acids and the peptides encoded thereby. The invention further relates to host-cells, stable cell lines and transgenic organisms comprising above-referenced nucleic acid molecules. The present invention also refers to methods of making and using small monomeric near-infrared fluorescent proteins derived from cyanobacteriochromes. The presented protein and its derivatives find use in a variety of applications and approaches, including labeling of biomolecules, cells or cell organelles, detecting protein-protein interactions, and generation of genetically encoded fluorescent biosensor.

The invention relates to a transgenic insect-resistant poplar survival competitive capacity evaluation method and application, and belongs to the technical field of transgenic poplar breeding and environmental safety evaluation. According to the characteristics of sexual and asexual propagation of poplar, transgenic insect-resistant poplar seeds and cutting slips are used as materials, the stability investigation of growth traits such as wasteland and cultivation land seed germination rate, cutting slip survival rate, plant height, ground diameter and the like and exogenous genes and the comparison of cutting slip storage modes are carried out, and a comprehensive evaluation method for the survival competitiveness of the transgenic insect-resistant poplar is established. Survival competitive capacity evaluation is an important part of transgenic organism safety evaluation, it is proved that transgenic poplar and non-transgenic poplar do not have the possibility of weed formation under wasteland conditions, and introduction of exogenous insect-resistant genes does not affect the survival competitive capacity of the poplar. The method solves the problem that there is no transgenic insect-resistant poplar survival competitive capacity evaluation method at present, and guarantees the safety evaluation of transgenic poplar organisms.

The present disclosure aims to provide a method for producing a trans-polyisoprenoid (trans-1,4-polyisoprene) with a molecular weight of more than 105 in an enzymatic manner. The present disclosure relate to a method for producing a trans-polyisoprenoid, which includes binding to a lipid membrane in vitro a trans-prenyltransferase (tPT) family protein capable of producing a product with a molecular weight of 104 or more when not bound to any lipid membrane.

The present invention relates to a CRISPR-Cas nickase system-based precise genome editing method. The genome editing method of the present invention can improve the precision of genome editing by reducing off-target effects, and thus can be effectively used in various fields such as new drug development, genetic disease treatment, genetically modified organism (GMO) development, and production of model animals used for organ transplantation.

The present invention relates to the field of biotechnology, specifically to ScGAME9 The invention discloses a gene for regulating the cold resistance of potatoes. ScGAME9 A gene, the nucleotide sequence of which is as shown in (a), (b) or (c), (a) the nucleotide sequence shown in SEQ ID NO.2; (b) a nucleotide sequence that hybridizes with and encodes the nucleotide sequence shown in SEQ ID NO.2; (c) a nucleotide sequence that has more than 80% homology with the nucleotide sequence shown in SEQ ID NO.2 and encodes. ScGAME9 Or its transgenic biological materials can regulate the cold tolerance of potato plants, providing important theoretical support for the screening of genetically transformed positive plants and the study of the mechanism of potato resistance to low temperature stress, and have broad application prospects.

The present invention relates to the field of genome-editing, more specific of difficult to transform organisms such as e.g. invertebrates and in particular Arthropoda. The present disclosure provides a combination comprising one or more cationic nanoparticles, one or more endosomal escape reagent(s), and one or more ribonucleoprotein (RNP) complexes wherein said RNP comprises a Cas RNA-guided nuclease associated with a guide RNA (gRNA), and the use thereof in the production of a non-human genetically modified organism. The invention further provides a combination as defined herein for use in CRISPR based methods.

This disclosure provides genetically engineered organisms with genetic modifications that are useful for producing desirable alkaloids. This disclosure also provides methods of making genetically engineered organisms that can produce desirable alkaloids. The organisms described herein produce alkaloids in amounts beyond that produced from comparable wild-type organisms. In certain embodiments, the genetically engineered organisms are fungi from the Basidiomycota division.

Provided are transgenic organisms, such as plants and plant parts, cells, and related compositions and methods for producing and monitoring the genotype for enhanced production of montbretin A and / or its precursors. For example, provided is a transgenic organism comprising at least one heterologous nucleic acid operatively linked to a promoter, wherein the heterologous nucleic acid encodes at least one enzyme in a montbretin A (MbA) metabolic pathway. The organisms can be a plant, plant part, or plant cell, or a microorganism such as a yeast. Also provided is a method for producing at least one montbretin A (MbA) precursor and / or MbA, comprising permitting the expression of the at least one heterologous nucleic acid in the transgenic organism. The disclosure also provides isolated nucleic acid molecules that comprise sequence encoding at least one enzyme in a montbretin A (MbA) metabolic pathway and vectors comprising the nucleic acids.

A control strategy for RNA-based bacterial nucleic acid amplification tests (NAATs), such as reverse transcription PCR (RT-PCR) and reverse transcription loop-mediated isothermal amplification (RT-LAMP), that allows ready discrimination of RNA from DNA templates using self-splicing bacterial introns, such that those nucleic acids ultimately encode different sequences. Described are two exemplary vectors encoding synthetic transgenes based on this principle, one that is active in the gram-negative bacterium Escherichia coli and one that functions in both E. coli and the gram-positive organism Staphylococcus aureus. Using multiplexed fluorescent RT-LAMP of heat-lysed specimens, the practicality of deploying such transgenic organisms as an internal control to ascertain sample integrity and assay performance during clinical diagnostic testing is demonstrated. The approach has broad utility for RNA-based bacterial nucleic acid amplification tests, especially point-of-care assays and other applications where nucleic acids are nonspecifically liberated for testing.

A process is disclosed for production of a polyhydroxyalkanoate that includes depolymerization of a post-consumer polyhydroxyalkanoate and utilization of the hydroxyalkanoate monomer thus produced as a carbon source for a microorganism capable of production of a polyhydroxyalkanoate. Methods can be utilized for true cyclic use of polyhydroxyalkanoates including polyhydroxybutyrates. Various aspects are described including simultaneous depolymerization and polymer production, utilization of purified depolymerase enzymes and / or microorganisms that express a depolymerase in conjunction with a microorganism that produces polymer, utilization of microorganisms that produce both a depolymerase and a new polymer, and utilization of genetically modified organisms to produce natural or modified depolymerase enzymes.

Provided is a computer implemented method for mapping transgene integration into n organism by providing a custom genome comprising a reference genome sequence and a transgene sequence, aligning sequence reads of the transgenic organism to the custom genome, identifying candidate junction reads, wherein the candidate junction reads correspond to sequence reads comprising sequence regions that align to the transgene sequence and to the host sequence, categorizing the candidate junction reads into junction clusters, wherein each of the junction clusters comprises a plurality of the candidate junction reads each with an alignment segment terminus within a cluster proximity region of the custom genome, determining a transgene junction based on the junction clusters that possesses at least three of the candidate junction reads within the cluster proximity region of the custom genome and mapping the integration of the transgene into the transgenic organism based on the transgene junctions.

The invention relates to a defoliating pest resistance evaluation method for insect-resistant transgenic poplar in a field environment and application, and belongs to the technical field of transgenic poplar breeding and environmental safety evaluation. According to the method, insect-resistant transgenic poplar and non-transgenic poplar are used as materials, a three-level classification standard based on damage area proportion is established through an orientation equilibrium sampling method by utilizing environmental monitoring and leaf damage detection, and fine evaluation of the defoliating insect resistance of the insect-resistant transgenic poplar is further realized. Field defoliating pest resistance evaluation is an important part of insect-resistant transgenic organism safety evaluation, and it is proved that insect-resistant transgenic poplars have good defoliating pest resistance. Through standardized sampling, high-precision detection and a scientific grading system, the problems of insufficient sample representativeness, inaccurate damage quantification and unscientific grading standard of an existing method for evaluating the field defoliating pest resistance of the transgenic insect-resistant poplar are solved, and a reliable basis is provided for biological safety evaluation and insect-resistant variety breeding of the transgenic poplar.

Polynucleotides comprising genetic regulatory elements, as well as constructs, host cells, and transgenic organisms comprising the same are described. The polynucleotides can control the expression of an operably linked gene in a host cell or organism, such as a plant cell or a plant. Methods of using the polynucleotide to control the expression of an operably linked gene of interest in a plant or plant cell are further provided.

Described herein are recombinant proteins comprising a sequence at least at least 70%, at least 80%, at least 85%, at least 90%, at least 95%, at least 97%, at least 99%, or 100% identical to SEQ ID NO:1, 2, or 3; and a secretion signal sequence heterologous thereto. The disclosure also provides genetically engineered microorganisms and fermentation methods for the production of said recombinant proteins.

Along with wide planting of transgenic crops, many countries start to carry out quantitative identification on transgenic products, but the prior art still faces huge challenges in the aspect of absolute quantitative detection, so that the invention provides a vortex-driven droplet digital CRISPR / Cas (VTD-CRISPR) detection method, and belongs to the field of nucleic acid detection. According to the invention, experimental parameters of the VTD-CRISPR technology are systematically optimized, so that the reliability and the accuracy of a detection result are ensured to the greatest extent; next, the detection specificity and sensitivity of the VTD-CRISPR are further verified by comparison with the commercially used qPCR and ddPCR technologies. In addition, the invention also explores the possibility that manual operation replaces vortex to generate liquid drops and is combined with a CRISPR (Clustered Regularly Interspaced Short Palindromic Repeats) technology. In conclusion, the VTD-CRISPR technology has an excellent qualitative function and an absolute quantitative function, and compared with other technologies, the VTD-CRISPR technology has the advantages of shorter detection time and lower cost, so that the VTD-CRISPR technology has great potential in practical application of quantitative detection of transgenic organisms.

The invention relates to a Burkholderia sp.S3-7 strain, which is characterized in that the Burkholderia sp.S3-7 strain is preserved in Guangdong Microbial Culture Collection Center on July 15, 2025, the preservation number is GDMCC NO.66685, and the preservation address is the 5th floor, No. 59 building, No. 100 Courtyard, Xianlie Middle Road, Guangzhou. The Burkholderia sp.S3-7 provided by the invention has relatively high degradation capacity and safety on collagen, can remarkably improve the yield of fish skin collagen peptide, does not have safety risks possibly brought by transgenic organisms, is high in public acceptability, is more suitable for being applied to the fields of food, health care products and the like, ensures the safety of the product and widens the application range of the product.

The present disclosure provides polypeptides having xanthan lyase activity, compositions, and uses thereof. The present disclosure also provides polynucleotides, expression vectors, host cells, and genetically modified organisms (e.g., bacteria) encoding xanthan lyases.

A control strategy for RNA-based bacterial nucleic acid amplification tests (NAATs), such as reverse transcription PCR (RT-PCR) and reverse transcription loop-mediated isothermal amplification (RT-LAMP), that allows ready discrimination of RNA from DNA templates using self-splicing bacterial introns, such that those nucleic acids ultimately encode different sequences. Described are two exemplary vectors encoding synthetic transgenes based on this principle, one that is active in the gram-negative bacterium Escherichia coli and one that functions in both E. coli and the gram-positive organism Staphylococcus aureus. Using multiplexed fluorescent RT-LAMP of heat-lysed specimens, the practicality of deploying such transgenic organisms as an internal control to ascertain sample integrity and assay performance during clinical diagnostic testing is demonstrated. The approach has broad utility for RNA-based bacterial nucleic acid amplification tests, especially point-of-care assays and other applications where nucleic acids are nonspecifically liberated for testing.

The present invention describes the expression cassette for transforming eukaryotic cell which comprises the peptide encoding non-natural sequence of nucleotides with xylose isomerase feature (SEQ ID NO: 1), optionally also comprising other genes of pentose phosphate route. Additionally, it is described the microorganism filed under the number DSM28739, which, in addition to the above-mentioned modifications, also present genetic modifications from adaptive evolution. The described microorganism shows efficient consumption of xylose and conversion of ethanol when compared to its correspondent without said genetic modifications and mutations from evolution. It is also described the process for producing biofuels e biochemicals, preferably ethanol, mainly from the lignocellulosic portion of the vegetal biomass. Biofuels, preferably ethanol, and biochemicals produced by the process of the invention are also described.

Systems and methods relate to transgenic organisms and their use as biosensors are described. In some embodiments, the systems and methods include a first population of transgenic organisms that includes a first constitutively expressed reporter gene, and a first transgene that includes a first inducible promoter from a response pathway gene, wherein the first inducible promoter is coupled to a first reporter gene. Other embodiments are described.

The present invention relates to the field of recombinant production of santalene and related products. In particular, the present invention relates to a method for producing a composition comprising at least one santalene, comprising a step of converting farnesyl pyrophosphate to at least one santalene, said conversion being carried out by a polypeptide exhibiting santalene synthase activity. Furthermore, the present invention contemplates a composition comprising a mixture of β-santalene and α-santalene, comprising an excess of β-santalene, obtainable by the method of the present invention. The present invention also relates to the use of a santalene synthase polypeptide, a heterologous polynucleotide encoding it, a vector or genetic construct comprising said polynucleotide, a host cell or a non-human transgenic organism comprising said genetic construct or vector, for producing a composition comprising at least one santalene, preferably β-santalene, more preferably a mixture of β-santalene and α-santalene. The present invention further relates to a method for producing a composition comprising at least one santalol, preferably β-santalol, comprising producing a composition comprising at least one santalene by the above-mentioned method of the present invention and oxidizing said at least one santalene, preferably β-santalene, to the respective alcohol to produce a composition comprising at least one santalol, preferably β-santalol.The present invention further relates to a kit for producing a composition comprising at least one santalene, comprising the above-mentioned polypeptide, heterologous polynucleotide, vector or genetic construct, host cell or non-human transgenic organism, and to a non-human host cell or non-human transgenic organism expressing a polypeptide exhibiting santalene synthase activity from the above-mentioned heterologous polynucleotide, vector or genetic construct.

A process is disclosed for production of a polyhydroxyalkanoate that includes depolymerization of a post-consumer polyhydroxyalkanoate and utilization of the hydroxyalkanoate monomer thus produced as a carbon source for a microorganism capable of production of a polyhydroxyalkanoate. Methods can be utilized for true cyclic use of polyhydroxyalkanoates including polyhydroxybutyrates. Various aspects are described including simultaneous depolymerization and polymer production, utilization of purified depolymerase enzymes and / or microorganisms that express a depolymerase in conjunction with a microorganism that produces polymer, utilization of microorganisms that produce both a depolymerase and a new polymer, and utilization of genetically modified organisms to produce natural or modified depolymerase enzymes.

This invention discloses a hybridoma cell line containing the herbicide-resistant protein GAT, its produced antibody, and its applications. The hybridoma cell line is deposited at the China General Microbiological Culture Collection Center (CGMCC), accession number CGMCC No. 46332. The preparation method includes: a) purifying prokaryotic expression to obtain recombinant GAT protein; b) immunizing animals: using the recombinant GAT protein as an antigen to immunize BALB / c mice; c) cell fusion: collecting spleen cells from immunized BALB / c mice and fusing them with SP2 / 0 cells; d) cell line establishment: subcloning using the limiting dilution method, performing ELISA detection on subclones after 5-7 days, until a stable hybridoma cell line secreting positive antibodies is selected for expansion, reculturing, and preservation. The monoclonal antibody secreted by the hybridoma cell line of this invention lays the foundation for the detection of the herbicide-resistant protein GAT in transgenic crops, thus supporting the development of transgenic crops. street The development, seed production, and preservation of genetically modified crops have ensured the survival of genetically modified organisms (GMOs). street The industrialization of genetically modified crops is progressing steadily, and the sustainable application of related transformants is advancing.

This invention relates to the field of genetic engineering. Specifically, it relates to cytosine deaminases and their use in base editing. More specifically, it relates to a method for screening and identifying deaminases, a base editing system based on newly identified cytosine deaminases, a method for base editing a target sequence in the genome of an organism (e.g., a plant) using this base editing system, and genetically modified organisms (e.g., plants) and their offspring produced by said method.

The invention belongs to the technical field of safety evaluation of soil microbiology and transgenic organisms, and particularly relates to a method for evaluating the influence of transgenic crops on soil microbial communities. According to the method, rhizosphere and rhizosphere soil samples before planting, in different growth periods and after harvesting of transgenic crops are collected, and the influence of the transgenic crops and non-transgenic control crops on soil microbial communities is evaluated through the steps of pretreatment, microbial culture, statistical analysis and the like. The method is suitable for a variety of crops, and is also suitable for comparative analysis of the influence of herbicide spraying on herbicide-resistant transgenic crop soil microflora. The method provides an efficient, simple, convenient and economical evaluation scheme for ecological safety evaluation of the transgenic crops, and has important significance for promoting sustainable utilization of the transgenic crops.