45 results about "Alicyclobacillus" patented technology

Tools and methods for detecting the presence bacteria, yeast and mold in a sample obtained from a food sample are provided. The methods employ a polymerase chain reaction and primer and probe sets that are based on the 16S rRNA and squalene-hopene cyclase genes of Alicyclobacillus and Geobacillus and the 18S rDNA gene of mold and yeast. The present invention also relates to primer and probe sets. Each primer and probe set comprises a forward primer and a reverse primer, both of which are from 15 to 35 nucleotides in length and a probe.

The invention relates to a new alicyclobacillus strain and an application thereof in bioleaching. The conservation number of the screened alicyclobacillus strain is CGMCC 4500; and the strain is cultured at the temperature of 25-35 DEG C and grows within the pH range of 1.5-5.0. The strain can be applied in the bioleaching field such as pyrite leaching. The alicyclobacillus strain has an acidophilic character, and can grow by taking ferrous ions as an energy matrix and fixed CO2 as a carbon source and meanwhile can rapidly grow by virtue of organicnutrition, thus the strain is suitable for large-scale amplification culture so as to be applied in the bioleaching field. Studies show that the new alicyclobacillus strain has the advantages of high leaching rate, short leaching time, no environmental pollution and the like, and metal ions therein have high tolerance and are free from inhibition of organic matters in the system.

The invention discloses an indirect ELISA (enzyme linked immunosorbent assay) kit for alicyclobacillus, which comprises components (1)-(10). Simultaneously, the invention further discloses a using method of the indirect ELISA kit for alicyclobacillus. The indirect ELISA kit for alicyclobacillus provided by the invention fills in the blank that an ELISA method against alicyclobacillus is not established in China, and further has higher sensitivity and specificity.

The invention relates to a microbial flue gas desulfurizer, which is characterized by comprising bacterial solution of active bacteria of Alicyclobacillus BM No.CGMCC 4500 and active bacteria of any one or more acidophilic bacteria which get energy from ferrous ions and/or reduced sulfur and/or element sulfur. Compared with the conventional pure-pyrolusite desulfurizer, the desulfurizer provided by the invention can improve the SO2 oxidization rate due to the addition of microbes which can oxide the reduced sulfur and fermentation liquor containing rich iron ions, improves the yield of manganese(II) dithionate serving as a byproduct and thus improves removal rate.

The invention relates to the gene engineering field, in particular to a xylanase XYNA4 and gene and application thereof. The invention provides a xylanase XYNA4 derived from alicyclic acid bacillus Alicyclobacillus hesperidumA4 (CGMCC No. 3147), amino acid sequence thereof is shown as SEQ ID NO. 1, and the invention also provides gene xynA4 coding the xylanase. The xylanase of the invention has the following properties: optimal pH is 7.0, optimal temperature is 55 DEG C, and specific activity is 4.20.2U/mg; and the xylanase has pH stability with extreme range, higher pH adaptability and better thermostability. The xylanase is used as a novel enzymic preparation can be widely applied to industries of animal feed, food, papermaking and energy sources.

The invention belongs to the field of metallurgical technology, and relates to a decarbonizing and desulphurizing bacterium agent for a difficultly-selected gold ore. The bacterium agent is formed by mixing alicyclobacillus, acidithiobacillus ferrooxidans, pseudomonas aeruginosa, bacillus subtilis, bacillus megatherium and burkholderia bacteria. The invention further discloses the method for treating the difficultly-selected gold ore by utilizing the decarbonizing and desulphurizing bacterium agent. The bacterium agent provided by the invention can be used for treating the high-carbon high-sulfur gold ore containing the sulfur content above 15% and the carbon content above 8%, and greatly lowering the quantity of sodium cyanide required for extracting the gold in a cyaniding manner. As a result, the industrial cost is lowered.

The invention discloses a microorganic aerobic composting method of kitchen garbage. The microorganic aerobic composting method comprises the following steps: water is used for cleaning kitchen waste so as to obtain an oil-water mixture and solid garbage; wood chips and alicyclobacillus are added into the solid garbage, and then aerobic composting is carried out so as to obtain a composting product. According to the invention, reducing, harmless and resourceful treatment to kitchen waste is carried out by adopting a microbic aerobic composting way. According to the invention, oil-water separation and reverse osmosis water treatment methods are adopted for treating kitchen waste, so as to reduce oil components in the kitchen waste and improve the composting effect, and the water resource reutilization is achieved. In the aerobic composting treating process, carbon-nitrogen ratio and water content are adjusted by adding cunninghamia lanceolata chips, the high-temperature-resistant and acid-proof alicyclobacillus are used, during linked optimization processes of heating, stirring and time controlling, energy conservation is achieved, the treatment efficiency is improved, the composting product meeting national standards and industrial standards is produced, and requirements of environment protection and sustainable development are met.

The invention relates to the field of gene engineering, and in particular relates to high-temperature resistance arabinfuranosidease Abf51B8, as well as a gene and an application thereof. The invention provides the arabinfuranosidease Abf51B8 coming from Alicyclobacillussp.B8 with an amino acid sequence shown as SEQ ID NO.1, and a coding gene abf51B8 for coding the arabinfuranosidease. The arabinfuranosidease has the characteristics of optimal pH of 6.0, optimal temperature of 60 DEG C and good thermal stability at 70 DEG C, and can be widely applied to feeds, foods, energy industries and the like serving as a novel enzyme preparation.

The invention discloses recombinant escherichia coli for heterologously synthesizing ambrein and a construction method thereof. The construction method comprises the following steps of: (1) fusing saccharomyces-cerevisiae squalene synthase gene ERG9 with truncation of 26 amino acid residues at a C end and homologous arms at the upstream and downstream of a lacZ site of the escherichia coli into adonor DNA; constructing plasmid 1; transforming the donor DNA and the plasmid 1 together into the escherichia coli, eliminating the plasmid 1, obtaining the recombinant escherichia coli for synthesizing squalene, and naming the recombinant escherichia coli as a strain 1; (2) connecting alicyclobacillus-acidocardarius squalene-hopene cyclase gene D377C SHC with mutation from the 377th amino acid residue into cysteine residue and bacillus-megaterium cyclase gene BmeTC into one segment, then inserting the segment into escherichia-coli expression plasmid p5C to obtain plasmid 4; (3) transforming the plasmid 4 into the strain 1 to obtain the recombinant escherichia coli for heterologously synthesizing the ambrein, and naming the recombinant escherichia coli as a strain 4. Proved by experiments,the recombinant escherichia coli for heterologously synthesizing the ambrein is fermented to obtain the ambrein.

The invention relates to a microbiological oxidation and reduction coupling leaching method for valuable metal in manganese oxide ore, characterized in that the leaching microbe is alicyclobacillus BM strains with the preservation number of CGMCC4500, and ferrous ions are added to the culture medium of the alicyclobacillus BM strains. In the invention, the leaching strain alicyclobacillus BM is used for leaching the valuable metal in the manganese oxide ore. The microbe not only can use carbon dioxide as a carbon source and use ferrous oxide ions to acquire energy, but also can use organic matters to realize quick and massive proliferation, thereby improving the leaching rate and the leaching effect, overcoming the defects of slow growth and small quantity of chemoautotrophic microbes, and enabling bioleaching to be applied to industrial leaching of low-grade manganese ore.

The invention discloses PCR (polymerase chain reaction) detection primers for three bacteria and the application of the PCR detection primers. The three bacteria are alicyclobacillus aacillus, escherichia coli and salmonella. The upstream detection primer of the alicyclobacillus aacillus has the SEQIDNO.1, and the downstream detection primer has the SEQIDNO. 2. The upstream detection primer of the escherichia coli has the SEQIDNO.3, and the downstream detection primer has the SEQIDNO.4. The upstream detection primer of the salmonella has the SEQIDNO.5, and the downstream detection primer has the SEQIDNO.6. The multiple PCR detection method provided by the invention has the biggest advantages that the one PCR tube can be used for detecting three bacteria, the PCR detection efficiency is improved, and the errors caused by misoperation during detection are reduced.

The invention discloses alicyclobacillus immunomagnetic microspheres and an application thereof. The disclosed alicyclobacillus immunomagnetic microspheres are obtained by coating aminated magnetic microspheres with oxidized alicyclobacillus specific antibodies. A preparation method of the oxidized alicyclobacillus specific antibodies comprises the following steps: alicyclobacillus specific antibodies are oxidized by using a PBS solution of sodium persulfate, and then the oxidized alicyclobacillus specific antibodies are collected to obtain. The disclosed alicyclobacillus immunomagnetic microspheres can be used for effectively separating alicyclobacillus in fruit juice especially in apple juice.

The invention discloses a method for quickly and specifically detecting Alicyclobacillus in apple juice through polymerase chain reaction (PCR). In the method, DNA is directly extracted from a juice sample by microwaves; a pair of specific primers are designed according to a gene sequence of squalene cyclase (Shc) of AlicyclobacillusacidoterrestrisDSM3922 provided by a GenBank; a 25ul system consisting of template DNA, a primer JLX-F and a primer JLX-R is used for PCR amplification; and a target strip is obtained at a position of 648bp through 1 percent agarose gel electrophoresis detection, and a detection result is obtained. The specificity of the primers, the amplification effect of microwave extracted DNA, and detection sensitivity are researched, and through inspection, the method has the advantages of short detection time and low detection cost and can realize online detection of apple juice production.

The invention discloses a method for treating printing and dyeing wastewater in textile mills, which comprises the following steps: S1: the printing and dyeing wastewater is first passed into a hydrolytic acidification pool, and the brevis and Alicyclobacillus inoculated in the hydrolytic acidification pool are used to treat the printing and dyeing wastewater The macromolecular organic matter in the water is degraded, and the wastewater treated in the hydrolytic acidification tank described in S2:S1 is passed into the anoxic tank, and the Bacillus cereus and Pseudomonas cerevisiae inoculated in the anoxic tank detoxify the wastewater Nitrogen treatment, S3: The wastewater treated in the anoxic tank described in S2 is passed into the anaerobic tank, and the ethanol thermophilic anaerobic bacteria inoculated in the anaerobic tank perform nitrogen and phosphorus removal treatment on the wastewater. The invention is economical and practical, and the sludge produced in the aerobic tank is sequentially processed through solid-liquid separation equipment, drying equipment, extrusion equipment and cutting equipment, and the sludge produced in the aerobic tank finally becomes small solid sludge , making the sludge produced in the aerobic tank easy to transport.

The invention relates to a chromogenic culture medium for detecting alicyclobacillus as well as application method and use thereof. The chromogenic culture medium comprises a component A, a component B and a component C, wherein the component A comprises the following substances in parts by weight: 2-4 parts of a yeast extract, 5-10 parts of glucose, 0.25-0.4 part of CaCl2.2H2O, 0.3-0.5 part of MgSO4.7H2O, 0.1-0.3 part of (NH4)2SO4 and 2.5-3.5 parts of KH2PO4; the component B comprises the following substances in parts by weight: 15-20 parts of agar and 0.08-0.12 part of beta-galactosidase substrate; and 1 part by weight of the component C comprises the following substances in parts by weight: 0.18*10<-3>-0.2*10<-3> part of ZnSO4.7H2O, 0.16*10<-3>-0.18*10<-3> part of CuSO4.5H2O, 0.15*10<-3>-0.17*10<-3> part of MnSO4.H2O, 0.18*10<-3>-0.2*10<-3> part of COCl2.6H2O, 0.1*10<-3>-0.12*10<-3> part of H3BO3 and 0.3*10<-3>-0.5*10<-3> part of NaMoO4.2H2O. The chromogenic culture medium providedby the invention has strong capability of supporting growth of the alicyclobacillus and can be used for detecting the target alicyclobacillus with high specificity.

Primers specific for an Alicyclobacillus spp. are used to amplify specific regions of 16S rDNA gene of the Alicyclobacillus spp., and the presence or absence of the amplified product is confirmed to detect the Alicyclobacillus spp. (especially Alicyclobacillus acidoterrestris).

The invention discloses a primer and an application thereof in PCR detection of alicyclobacillus spp. in fruit juice. The method comprises the following steps: acquiring the specific primer according to the design of a conserved domain of a 16S rDNA gene sequence of the alicyclobacillus spp.; establishing an SYBR Green I fluorescent quantitative PCR method system; carrying out amplification on a standard positive sample, establishing a standard curve and drawing a solubility curve; carrying out immunomagnetic separation and DNA extraction on the alicyclobacillus spp. in a fruit juice sample; and carrying out analysis and judgment on the result. According to the invention, the established fluorescent quantitative detection method can detect ninety-six samples within three hours, and has the characteristics of rapidness and simplicity, low detection cost, simplicity in realizing standard operation, good accuracy, and high sensitivity.

The invention relates to a production method of high fructose corn syrup without alicyclobacillus acidoter-restris. Starch milk is subjected to a series of treatment such as high-concentration liquefaction, saccharification, isomerization, chromatography, refining, membrane filtration, sterilization filtration, concentration and the like, and orderly cleaning and disinfection measures are taken for all procedures in the production process, so that the final production of the high fructose corn syrup product without alicyclobacillus acidoter-restris is ensured. The method has the advantages that: the high-concentration liquefaction is adopted for liquefaction, so that a certain bacteriostatic effect and an energy-saving effect are achieved; in order to ensure the introduction risk of alicyclobacillus acidoter-restris of source feed liquid, the high-temperature sterilization is carried out at a spray liquefaction temperature of 125-148 DEG C in the liquefaction process; and in addition,certain disinfection measures are made for isomerization and chromatography processes in the production process, so that alicyclobacillus acidoter-restris is prevented from breeding in the productionprocess, and the dead corner for alicyclobacillus acidoter-restris breeding is avoided as much as possible in the production process. The process is simple, convenient and feasible, facilitates industrial production control, and improves the quality of high fructose corn syrup products.

The invention provides a method for controlling the pollution of Alicyclobacillus in water, which sterilizes and irradiates Alicyclobacillus in water with medium-pressure ultraviolet rays, thereby preventing the bacteria from entering subsequent production links from the source and causing cross-contamination among production equipment, furthermore thereby ensuring the quality of end products of fruit and vegetable industries. The medium-pressure ultraviolet light is combined with hydrogen peroxide, the method can properly reduce the irradiation dose of the medium-pressure ultraviolet ray on the premise of obtaining better sterilization effect, does not need to introduce new chemical substances in the sterilization process, and is safer to use in the food production and processing process,and has good application prospect especially in treating the processing and cleaning water of fruits and vegetables and other production water.

The invention discloses an alicyclobacillus aeris for degrading an anionic surfactant. The alicyclobacillus aeris is alicyclobacillus aeris A-YW and is preserved in the China Center for Type Culture Collection (CCTCC) on April 25, 2021, the strain preservation number of the alicyclobacillus aeris is CCTCC M 2021451, and the preservation address of the alicyclobacillus aeris is Luojia mountain on bayi road of Wuchang District, Wuhan City, Hubei Province. According to the invention, the sludge collected from a sewage outlet of a detergent manufacturer is separated to obtain alicyclobacillus aeris A-YW, an anionic surfactant degradation test shows that the strain has a good degradation effect on anionic surface activity; the strain is an environment-friendly strain with potential significance in sewage treatment, and the method has the characteristics of high degradation efficiency, high degradation rate and the like, and provides help for environmental protection in China.

The invention provides a method for synchronously controlling alicyclobacillus in water and guaiacol as an odor metabolism pollutant of the alicyclobacillus. Medium-pressure ultraviolet rays are adopted to sterilize and irradiate alicyclobacillus in water, so that the alicyclobacillus is prevented from entering a subsequent production link to cause cross contamination among production equipment from the source, and therefore, the quality of terminal products in the fruit and vegetable industry is ensured. Medium-pressure ultraviolet rays and potassium persulfate are combined for use, so that the irradiation dose of the medium-pressure ultraviolet rays can be properly reduced on the premise of ensuring a relatively good sterilization effect, a large amount of harmful disinfection byproducts cannot be generated in a treatment process, the method is safer to use in a food production and processing process, and particularly, the method has a relatively good application prospect in the aspects of processing and cleaning water for fruits and vegetables and disinfecting water for other foods.

The invention relates to an alicyclobacillus colloidal gold label test strip. The alicyclobacillus colloidal gold label test strip comprises a lining plate, and is characterized in that the lining plate is coated with a double-faced adhesive tape layer, wherein one end of the lining plate is provided with a handheld end water absorption layer, and the other end of the lining plate is provided with a test end water absorption layer; the lining plate between the handheld end water absorption layer and the test end water absorption layer is sequentially provided with a detection layer and an anti-alicyclobacillus gold label antibody layer; one end of the detection layer is bonded with the handheld end water absorption layer, the other end of the detection layer is bonded with the anti-alicyclobacillus gold label antibody layer, and the anti-alicyclobacillus gold label antibody layer is bonded with the test end water absorption layer; the detection layer is sequentially provided with a detection line and a control line along the flow direction of a sample; the handheld end water absorption layer and the test end water absorption layer are respectively provided with a single-faced adhesive tape protection layer. The invention also discloses a preparation method of the alicyclobacillus colloidal gold label test strip. The alicyclobacillus colloidal gold label test strip has characteristics of high specificity, high sensitivity, and simplicity and convenience in operation.