Decarbonizing and desulphurizing bacterium agent for difficultly-selected gold ore and application thereof
A desulfurization bacteria, gold mine technology, applied in the direction of bacteria, process efficiency improvement, microorganism-based methods, etc., can solve problems such as poor carbide effect, reduce industrial energy consumption, avoid environmental pollution, and have good tolerance Effect
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Embodiment 1
[0029] A decarburization and desulfurization bacterial agent for refractory gold ore, which is formed by mixing the following raw materials in parts by weight:
[0030] Alicyclobacillus 10 parts, acidophilus Thiobacillus ferrooxidans 8 parts, Pseudomonas aeruginosa 6 parts, Bacillus subtilis 4 parts, Bacillus megaterium 4 parts, Burkholderia 3 parts.
[0031] The Alicyclobacillus is Alicyclobacillus, CGMCC4500 (refer to CN2011100247602 public use), the culture medium of Alicyclobacillus is BAM medium, cultivated at 30°C until the number of viable bacteria reaches 1.0×10 8pcs / g.
[0032] The acidophilus ferrooxidans is Acidithiobacillus ferrooxidans ATCC 53993 (see A genomic island provides Acidithiobacillus ferrooxidans ATCC 53993 additional copper resistance: a possible competitive advantage. Appl Microbiol Biotechnol. 2011); The acidophilic Thiobacillus ferrooxidans in 9K medium ((NH 4 ) SO 4 3g / L, KCl 0.1g / L, K 2 HPO 4 0.5g / L, Ca(NO 3 ) 2 0.01g / L, FeSO 4 ·7H 2 O...
Embodiment 2
[0038] The gold ore selected as the material is a carbonaceous gold ore in Gansu Province. The main metallic minerals are pyrite and chalcopyrite, etc. The main non-metallic minerals are mica, followed by quartz, graphite and carbon. The main element analysis results of the ore samples are shown in Table 1.
[0039] Table 1 Analysis of each element composition
[0040] element Fe Cu S C As Au content% 20.1 0.69 15.1 8.3 2.1 0.0048
[0041] A method for processing refractory gold ore using the bacterial agent prepared in embodiment 1, it may further comprise the steps:
[0042] The refractory gold ore is crushed by a crusher, then sent to a ball mill, and ground to a particle size of 100 mesh ore powder, wherein an activator is added to the ball mill, and the amount added is one thousandth of the mass of the gold ore; the activator is in accordance with Sodium silicate: carbon tetrachloride is mixed at a mass ratio of 1:1; water is added to...
Embodiment 3
[0046] Bacteria effect test:
[0047] It is divided into four groups, wherein the comparative test is divided into three groups, specifically the control group 1, the control group 2, the control group 3, and the experimental group is the embodiment 2 group of the present invention.
[0048] Control group 1: The bacterial agent is prepared from acidophilus Thiobacillus ferrooxidans and acidophilic sulfur bacteria according to the ratio of 1:1, the dosage of sodium cyanide is 5kg / t, and the rest is the same as in Example 2; Control group 2: The bacterial agent is Alicyclobacillus, Acidithiobacillus and Leptospira ferrophilum were prepared according to the ratio of 2:1:1, the amount of sodium cyanide was 2kg / t, and the rest were the same as in Example 2; Control 3: no activator was added , the processing method is the same as in Example 2. The desulfurization rate, decarburization rate and gold leaching rate were obtained through detection, see Table 2.
[0049] Table 2
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