48 results about "Alicyclobacillus pohliae" patented technology

A genetically modified organism comprising: at least one nucleic acid sequence and/or at least one recombinant nucleic acid isolated from Alicyclobacillus acidocaldarius and encoding a polypeptide involved in at least partially degrading, cleaving, transporting, metabolizing, or removing polysaccharides, cellulose, lignocellulose, hemicellulose, lignin, starch, sugars, sugar oligomers, carbohydrates, complex carbohydrates, chitin, heteroxylans, glycosides, xylan-, glucan-, galactan, or mannan-decorating groups; and at least one nucleic acid sequence and/or at least one recombinant nucleic acid encoding a polypeptide involved in fermenting sugar molecules to a product. Additionally, enzymatic and/or proteinaceous extracts may be isolated from one or more genetically modified organisms. The extracts are utilized to convert biomass into a product. Further provided are methods of converting biomass into products comprising: placing the genetically modified organism and/or enzymatic extracts thereof in fluid contact with polysaccharides, cellulose, lignocellulose, hemicellulose, lignin, starch, sugars, sugar oligomers, carbohydrates, complex carbohydrates, chitin, heteroxylans, glycosides, and/or xylan-, glucan-, galactan, or mannan-decorating groups.

The invention relates to a new alicyclobacillus strain and an application thereof in bioleaching. The conservation number of the screened alicyclobacillus strain is CGMCC 4500; and the strain is cultured at the temperature of 25-35 DEG C and grows within the pH range of 1.5-5.0. The strain can be applied in the bioleaching field such as pyrite leaching. The alicyclobacillus strain has an acidophilic character, and can grow by taking ferrous ions as an energy matrix and fixed CO2 as a carbon source and meanwhile can rapidly grow by virtue of organicnutrition, thus the strain is suitable for large-scale amplification culture so as to be applied in the bioleaching field. Studies show that the new alicyclobacillus strain has the advantages of high leaching rate, short leaching time, no environmental pollution and the like, and metal ions therein have high tolerance and are free from inhibition of organic matters in the system.

The invention relates to pullulanase producing strain and manufacturing method. It is named alieycloeacillus. And its preservation register number is CGMCC No.1504. The formed strain D-1 is one new species of alieycloeacillus. Its pullulanase pH is 3.5-4.5. If pH is higher then 5.0 or lower than 3.0, enzyme is inactive; if it is 4.0, residue enzyme energy is 88%; the optimum temperature is 60 centigrade degree; enzyme reaction is not need metal ion while temperature is 55-65 centigrade degree. Thus the enzyme can be used in starch processing saccharification stage. Compared with acidity pullulanase, the enzyme has better heat resistance and pH stability.

The invention discloses an indirect ELISA (enzyme linked immunosorbent assay) kit for alicyclobacillus, which comprises components (1)-(10). Simultaneously, the invention further discloses a using method of the indirect ELISA kit for alicyclobacillus. The indirect ELISA kit for alicyclobacillus provided by the invention fills in the blank that an ELISA method against alicyclobacillus is not established in China, and further has higher sensitivity and specificity.

The invention belongs to the field of metallurgical technology, and relates to a decarbonizing and desulphurizing bacterium agent for a difficultly-selected gold ore. The bacterium agent is formed by mixing alicyclobacillus, acidithiobacillus ferrooxidans, pseudomonas aeruginosa, bacillus subtilis, bacillus megatherium and burkholderia bacteria. The invention further discloses the method for treating the difficultly-selected gold ore by utilizing the decarbonizing and desulphurizing bacterium agent. The bacterium agent provided by the invention can be used for treating the high-carbon high-sulfur gold ore containing the sulfur content above 15% and the carbon content above 8%, and greatly lowering the quantity of sodium cyanide required for extracting the gold in a cyaniding manner. As a result, the industrial cost is lowered.

The invention discloses recombinant escherichia coli for heterologously synthesizing ambrein and a construction method thereof. The construction method comprises the following steps of: (1) fusing saccharomyces-cerevisiae squalene synthase gene ERG9 with truncation of 26 amino acid residues at a C end and homologous arms at the upstream and downstream of a lacZ site of the escherichia coli into adonor DNA; constructing plasmid 1; transforming the donor DNA and the plasmid 1 together into the escherichia coli, eliminating the plasmid 1, obtaining the recombinant escherichia coli for synthesizing squalene, and naming the recombinant escherichia coli as a strain 1; (2) connecting alicyclobacillus-acidocardarius squalene-hopene cyclase gene D377C SHC with mutation from the 377th amino acid residue into cysteine residue and bacillus-megaterium cyclase gene BmeTC into one segment, then inserting the segment into escherichia-coli expression plasmid p5C to obtain plasmid 4; (3) transforming the plasmid 4 into the strain 1 to obtain the recombinant escherichia coli for heterologously synthesizing the ambrein, and naming the recombinant escherichia coli as a strain 4. Proved by experiments,the recombinant escherichia coli for heterologously synthesizing the ambrein is fermented to obtain the ambrein.

The present invention discloses a method for increasing a metal ore leaching rate, and a special strain thereof. According to the present invention, Alicyclobacillus sp. SJ-68 is provided, the preservation number is CGMCC No.7682, and a bacterial agent for leaching the target metal from metal ore is further provided, and comprises Alicyclobacillus sp. SJ-68 described in the claim 1 and Acidithiobacillus caldus SM-1 described in the claim 1; and experiment results prove that the Alicyclobacillus sp. SJ-68CGMCC No.7682 can be separately used for leaching the valuable metal in the sulphide ore or be synergistically used with other strains so as to co-leach the valuable metal in the sulphide ore, can be used for deepening extraction of noble metals or rare metals in sulphide ore concentrates, abandoned mine, lean ore, metallurgical slag and difficultly-treated complex sulphide ores, and has important industrial application prospects in the biological mineral leaching field.

The invention provides an oligographic bacterium composition. The strain category name of the oligographic bacterium composition is alicyclobacillus sp., and the strain codes are as follows: Biometek-A, Biometek-B and Biometek-AP-AC. The Depository Authority is China General Microbiological Culture Collection Center (CGMCC) and the address is Institute of Microbiology of Chinese Academy of Sciences, 3#, No.1 Yard, West Beichen Road, Chaoyang District, Beijing; the preservation date is December 26, 2012 and the preservation numbers are as follows: CGMCC No.7038, CGMCC No.7039 and CGMCC No.7040; and the bacterium composition is formed by mixing the strains in the ratio of 1 to 1 to 1. According to the composition provided by the invention, an additional carbon source can be utilized for reducing ferric ions in a system, so that the generation of jarosite is inhibited.

The invention relates to a microbiological oxidation and reduction coupling leaching method for valuable metal in manganese oxide ore, characterized in that the leaching microbe is alicyclobacillus BM strains with the preservation number of CGMCC4500, and ferrous ions are added to the culture medium of the alicyclobacillus BM strains. In the invention, the leaching strain alicyclobacillus BM is used for leaching the valuable metal in the manganese oxide ore. The microbe not only can use carbon dioxide as a carbon source and use ferrous oxide ions to acquire energy, but also can use organic matters to realize quick and massive proliferation, thereby improving the leaching rate and the leaching effect, overcoming the defects of slow growth and small quantity of chemoautotrophic microbes, and enabling bioleaching to be applied to industrial leaching of low-grade manganese ore.

The invention discloses alicyclobacillus immunomagnetic microspheres and an application thereof. The disclosed alicyclobacillus immunomagnetic microspheres are obtained by coating aminated magnetic microspheres with oxidized alicyclobacillus specific antibodies. A preparation method of the oxidized alicyclobacillus specific antibodies comprises the following steps: alicyclobacillus specific antibodies are oxidized by using a PBS solution of sodium persulfate, and then the oxidized alicyclobacillus specific antibodies are collected to obtain. The disclosed alicyclobacillus immunomagnetic microspheres can be used for effectively separating alicyclobacillus in fruit juice especially in apple juice.

An antimicrobial agent including hop acids is disclosed that inhibits the growth of acid-resistant and heat-resistant bacteria such as Alicyclobacillus acidoterrestris and Alicyclobacillus acidocaldarius in a medium such as fruit juice.

The invention discloses a method for treating printing and dyeing wastewater in textile mills, which comprises the following steps: S1: the printing and dyeing wastewater is first passed into a hydrolytic acidification pool, and the brevis and Alicyclobacillus inoculated in the hydrolytic acidification pool are used to treat the printing and dyeing wastewater The macromolecular organic matter in the water is degraded, and the wastewater treated in the hydrolytic acidification tank described in S2:S1 is passed into the anoxic tank, and the Bacillus cereus and Pseudomonas cerevisiae inoculated in the anoxic tank detoxify the wastewater Nitrogen treatment, S3: The wastewater treated in the anoxic tank described in S2 is passed into the anaerobic tank, and the ethanol thermophilic anaerobic bacteria inoculated in the anaerobic tank perform nitrogen and phosphorus removal treatment on the wastewater. The invention is economical and practical, and the sludge produced in the aerobic tank is sequentially processed through solid-liquid separation equipment, drying equipment, extrusion equipment and cutting equipment, and the sludge produced in the aerobic tank finally becomes small solid sludge , making the sludge produced in the aerobic tank easy to transport.

The invention discloses a primer and an application thereof in PCR detection of alicyclobacillus spp. in fruit juice. The method comprises the following steps: acquiring the specific primer according to the design of a conserved domain of a 16S rDNA gene sequence of the alicyclobacillus spp.; establishing an SYBR Green I fluorescent quantitative PCR method system; carrying out amplification on a standard positive sample, establishing a standard curve and drawing a solubility curve; carrying out immunomagnetic separation and DNA extraction on the alicyclobacillus spp. in a fruit juice sample; and carrying out analysis and judgment on the result. According to the invention, the established fluorescent quantitative detection method can detect ninety-six samples within three hours, and has the characteristics of rapidness and simplicity, low detection cost, simplicity in realizing standard operation, good accuracy, and high sensitivity.

The invention relates to a primer and a probe for alicyclobacillus acidoterrestris PCR detection and applications thereof. The primer and the probe are designed according to a conserved domain of an A.a cidoterrestris16S rDNA gene, and are used for quantitatively detecting the nucleic acid copy number of A.a cidoterrestris in a sample to be detected. Specifically, an upstream primer of the primer has a nucleotide sequence of EQ ID NO.1 in a sequence table, and a downstream primer has a nucleotide sequence of EQ ID NO.2 in the sequence table; the probe has a nucleotide sequence of EQ ID NO.3 in the sequence table. A related method is implemented by taking the total DNA of a sample as a template through carrying out real-time fluorescent PCR amplification by using the primer and the probe, acquiring data after each cycle is completed, and determining results according to an amplification curve after the reaction is reacted. The probe disclosed by the invention has the characteristics of good specificity, rapid detection speed, good accuracy and high sensitivity.

The invention relates to a preparation method of an alicyclic acid bacillus resisting monoclonal antibody. The method comprises the following steps: (1) preparing an immunized mouse; (2) culturing myeloma cells for 3-4 days to obtain cultured mouse myeloma cells; (3) inoculating alicyclic acid bacillus in the abdominal cavity of the immunized mouse for immunization and obtaining spleen cells; dispersing the spleen cells to obtain a spleen cell precipitate; (4) centrifuging the cultured mouse myeloma cells to obtain a myeloma cell precipitate; (5) respectively suspending and mixing the spleen cell precipitate and the myeloma cell precipitate in a DMEM high-sugar culture solution, and centrifuging to obtain a precipitate; centrifuging and suspending the precipitate to obtain fused cells; (7) culturing and assaying the fused cells to determine hybridoma cells; cloning and screening the hybridoma cells to obtain a hybridoma cell line; and (8) preparing a monoclonal antibody, namely performing monoclonal antibody production on the hybridoma cell line according to a general method. The preparation method disclosed by the invention is rapid and high in specificity.

Isolated and/or purified polypeptides and nucleic acid sequences encoding polypeptides from Alicyclobacillus acidocaldarius are provided. Further provided are methods of at least partially degrading, cleaving, or removing polysaccharides, lignocellulose, cellulose, hemicellulose, lignin, starch, chitin, polyhydroxybutyrate, heteroxylans, glycosides, xylan-, glucan-, galactan-, or mannan-decorating groups using isolated and/or purified polypeptides and nucleic acid sequences encoding polypeptides from Alicyclobacillus acidocaldarius.

The invention provides a method for controlling the pollution of Alicyclobacillus in water, which sterilizes and irradiates Alicyclobacillus in water with medium-pressure ultraviolet rays, thereby preventing the bacteria from entering subsequent production links from the source and causing cross-contamination among production equipment, furthermore thereby ensuring the quality of end products of fruit and vegetable industries. The medium-pressure ultraviolet light is combined with hydrogen peroxide, the method can properly reduce the irradiation dose of the medium-pressure ultraviolet ray on the premise of obtaining better sterilization effect, does not need to introduce new chemical substances in the sterilization process, and is safer to use in the food production and processing process,and has good application prospect especially in treating the processing and cleaning water of fruits and vegetables and other production water.