Alicyclobacillus immunomagnetic microspheres and application thereof

Abstract

The invention discloses alicyclobacillus immunomagnetic microspheres and an application thereof. The disclosed alicyclobacillus immunomagnetic microspheres are obtained by coating aminated magnetic microspheres with oxidized alicyclobacillus specific antibodies. A preparation method of the oxidized alicyclobacillus specific antibodies comprises the following steps: alicyclobacillus specific antibodies are oxidized by using a PBS solution of sodium persulfate, and then the oxidized alicyclobacillus specific antibodies are collected to obtain. The disclosed alicyclobacillus immunomagnetic microspheres can be used for effectively separating alicyclobacillus in fruit juice especially in apple juice.

Description

technical field

[0001] The invention belongs to the technical field of food microorganism detection, and in particular relates to immunomagnetic microspheres of Alicyclobacillus and applications thereof. Background technique

[0002] Alicyclobacillus, commonly known as acidophilic and thermostable bacteria, belongs to Gram-positive bacillus, rod-shaped, spore-forming, strictly aerobic, and can survive and grow in a wide temperature range of pH 2.5-6.0 and 20-60°C , is a common contaminating bacteria in apple and other fruit juices. Due to its heat resistance and acid resistance, ordinary pasteurization is difficult to kill it. The bacterium does not multiply in concentrated fruit juice, but when the concentrated fruit juice is diluted into low-concentration fruit juice, it can metabolize and produce substances with unpleasant flavors - guaiacol and 2,6-dibromophenol, which make the taste and flavor of the juice worse , forming a white precipitate or foggy turbidity, which ...

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