122 results about "Guaiacol" patented technology

The invention belongs to the technical field of white spirit brewing, and particularly relates to bacillus velezensis BL-LM and application thereof in preparation of special-flavor white spirit yeast. The bacterial strain is classified and named as Bacillus velezensis, and is preserved in the China General Microbiological Culture Collection Center (CGMCC), and the preservation number is CGMCC No.30130. The invention further discloses a preparation method of the bacterial strain. When the bacillus velezensis BL-LM is added into starter propagation raw materials, in the starter propagation process, the top fire temperature of a starter blank is high, the temperature rising time is long, the liquefying power and saccharifying power activity of the starter blank is higher, the capacity of producing white spirit characteristic flavor substances such as pyrazines, guaiacol and 4-vinyl guaiacol is higher, and the content of organic acid is high. The bacillus velezensis BL-LM disclosed by the invention has a relatively good promotion effect on physicochemical property optimization and flavor substance generation of the specially-fragrant yeast, and has a very obvious effect in the aspect of modifying the quality of the specially-fragrant yeast.

The invention provides a catalyst for preparing phenol through catalytic hydrogenation of guaiacol as well as a preparation method and application of the catalyst. The preparation method of the catalyst comprises the following steps: fully dissolving magnesium nitrate, P123 and urea in deionized water, carrying out hydrothermal reaction, filtering, washing, drying and roasting to obtain an MgO carrier; immersing the MgO carrier in an alkali metal nitrate aqueous solution, drying and roasting to obtain an alkali metal element loaded MgO carrier; the alkali metal is selected from one of Li, Na, K or Cs; and immersing the alkali metal element loaded MgO carrier in a methanol solution of chloroplatinic acid, removing methanol, drying, and then carrying out reduction treatment to obtain the catalyst. The catalyst disclosed by the invention is applied to catalytic hydrodeoxygenation of guaiacol and shows excellent catalytic performance, the hydrodeoxygenation reaction conditions are mild, and the target product phenol can be obtained with high selectivity and high yield.

The invention provides a cronobacter malonate strain and application thereof, the strain is preserved in China Center for Type Culture Collection on January 15, 2025, the preservation date is January 15, 2025, and the preservation number is CCTCC M 2025146. The strain has excellent capability of producing 4-vinyl guaiacol, and the yield in a sorghum juice fermentation culture medium is 2.70 + / -0.15 mg / L; meanwhile, the strain can resist ferulic acid and has good capability of converting ferulic acid to generate 4-vinyl guaiacol, and when 0.1% (w / w) of ferulic acid is added into a fermentation culture medium, the content of 4-vinyl guaiacol reaches 2.68 + / -0.04 g / L. The strain is applied to production of Maotai-flavor liquor, the content of 4-ethyl guaiacol in raw liquor reaches 3.20 + / -0.26 mg / L and is remarkably increased by 223.23% (P is smaller than 0.01) compared with the content of 4-ethyl guaiacol without the strain, the Maotai flavor is prominent through sensory evaluation, the flower, fruit and burnt flavor is obvious, and the strain has important significance on improvement of the health quality of the liquor.

The invention discloses Klebsiella and application thereof, and belongs to the technical field of microorganisms. According to the invention, firstly, a strain LLMJ-DNXJ-01 with high yield of tannase is screened from wine-making yeast, the identification result shows that the strain belongs to klebsiella, then the tannase activity of the strain is investigated, and the result shows that the enzyme activity reaches 8.8 + / -0.02 U / mL. In addition, the invention also investigates the application of the strain in degrading sorghum tannin, the sorghum tannin degradation rate reaches 34.95%, and isoamyl alcohol, isopentenol, caryophyllene, phenethyl alcohol, 4-vinyl guaiacol and other volatile aroma substances with Daqu characteristics can be generated through fermentation. Therefore, by integrating the tannase production capability of the strain LLMJ-DNXJ-01 and the sorghum tannin degradation and fermentation aroma production capability, the strain has relatively good development potential in the aspects of manufacturing of reinforced yeast for making hard liquor and quality improvement and aroma enhancement of white spirit.

The invention belongs to the technical field of microbial fermentation, and particularly relates to a new enterobacter rorrhizae strain as well as a separation and screening method and application thereof. In order to enrich microbial resources for producing pyrazine substances through metabolism in the white spirit industry, an enterobacter rorrhizae strain is separated and screened from a mixture of yeast for making hard liquor and fermented grains, and the preservation number is CGMCC No.33262. The strain has good tolerance to pH, salt and alcohol, and can adapt to the growth environment in the white spirit production process; and various pyrazine substances represented by tetramethylpyrazine can be produced. Besides, the strain has strong adaptability to fermented grains, the yield of tetramethylpyrazine is high in a culture medium taking bran, wheat and five grains as the fermented grains, and meanwhile, various flavor substances such as ethyl acetate, ethyl hexanoate, ethyl caprylate, 2, 3-butanediol, guaiacol and 4-vinyl guaiacol can be produced. Therefore, the enterobacter rorrhizae disclosed by the invention can be widely used for brewing white spirit.

The invention relates to a composition with functions of protecting gingiva and repairing enamel and a preparation method of the composition, and belongs to the technical field of oral care. The composition contains a functional additive, a plant compound fermentation extract and the like, the functional additive is a mixture of corn starch, calcium phosphosilicate, hydroxyapatite, ubiquinone, sodium guaiazulene sulfonate, menthol and vitamin E, and the plant compound fermentation extract is obtained by fermenting and purifying honeysuckle flowers, radix polygonati officinalis and dandelions according to a specific proportion. Through the synergistic effect of the fermentation extract and the functional components, the problems that an existing toothpaste body is hardened, the storage stability is poor, the gingival protecting effect is short, and the enamel repairing persistence is insufficient are solved, the gingival protecting effect can be enhanced, the enamel repairing effect can be improved, the stability of the toothpaste body can be optimized, the antibacterial property and the oral mucosa relieving effect are achieved, and the oral cavity repairing effect is achieved. And the preparation process is scientific and reasonable, and is suitable for daily oral care.

The invention relates to the technical field of insecticides, in particular to an insecticide composition for solanaceous vegetables and a preparation method thereof.The insecticide composition is prepared from, by mass, 92-95 parts of composite emulsion, 3-5 parts of loaded particles and 0.5-1 part of synergist; the composite emulsion is prepared from sodium alginate, flurala sodium, guaiacol, solvent oil, alkyl glycoside and deionized water; the sodium alginate is obtained by mixing ultra-high molecular weight sodium alginate and low molecular weight sodium alginate in a mass ratio of 9: (1-1.5); the loaded particles are prepared from polysuccinimide, D-glutamic acid, fluralan, guaiacol and sodium dodecyl sulfate; the synergist is prepared from esterified starch, esterified chitosan, glycerol and 2-hydroxy-4 '-(2-hydroxyethoxy)-2-methyl propiophenone, and the synergist is prepared from the esterified starch, the esterified chitosan, the glycerol and the 2-hydroxy-4'-(2-hydroxyethoxy) Therefore, the pesticide composition disclosed by the invention has higher pesticide effect stability, longer lasting period and wider application prospect in areas with larger temperature difference in the morning and evening and dry environment.

The invention discloses a body acne-removing composition containing guaiacol extract and application of the body acne-removing composition, and relates to the technical field of cosmetics. The invention provides an acne removing composition. The acne removing composition is prepared from the following components in parts by weight: 0.01 to 0.1 part of saponin A, 0.1 to 1 part of saponin B, 5 to 15 parts of buckwheat extract, 1 to 10 parts of cassia occidentalis seed extract and 1 to 10 parts of guaiacol extract. The saponin A comprises asperuloside and aucubin, the saponin B is ziyuglycoside, and the ziyuglycoside comprises ziyuglycoside I and ziyuglycoside II. When the saponin B and the saponin A cooperate with the buckwheat extract, the guaiacol extract and the cassia seed extract, on one hand, the whole acne-removing composition has excellent effects of removing heat, detoxifying and slowing down heat sensation, and can effectively prevent acne from growing on the skin; on the other hand, permeation of active substances in a skin barrier is promoted, dry skin is relieved, healing and repairing of existing acnes are accelerated, and the grease amount of the skin and the surface roughness of the skin are further improved.

The invention relates to the technical field of catalytic materials, in particular to a catalyst and a preparation method and application thereof. The invention provides a catalyst. The catalyst comprises organosilane modified mesoporous silica and nickel-molybdenum bimetallic loaded in the organosilane modified mesoporous silica. Nickel in the catalyst can promote hydrogen activation and hydrogenation, and molybdenum plays a role in catalytic cracking reaction. On the catalyst, the synergistic effect generated by combination of bimetal and acidic sites is more beneficial to efficient HDO reaction (catalytic hydrodeoxygenation reaction). The result of the embodiment shows that when the conversion rate of the model compound guaiacol is 100%, the highest selectivity of the obtained product naphthenic alcohol can reach 50.1%; when the catalyst is applied to the hydrodeoxygenation conversion reaction process of biomass alcoholysis derivative oil, the yield of naphthenic alcohol is greatly increased, and the relative content is increased from 3.5% to 49.8%.

The invention discloses a method for brewing high-quality antioxidant Luzhou-flavor liquor in a semi-solid state. The method comprises the following steps: separating glutinous sorghum serving as a wine brewing raw material into sorghum rice and sorghum peel through rice milling, recovering residual starch in the sorghum peel by adopting alpha-amylase and saccharifying enzyme, releasing different tannins by adopting cellulase, and depolymerizing tannin dissolved in the sorghum peel and a solution by adopting laccase to release tannin with different structures, so as to obtain tannin enzymatic hydrolysate; the enzymatic hydrolysate is added into the steamed peeled sorghum rice according to a certain proportion, so that the thermal destructive effect of the steamed material on tannin in the sorghum rice is avoided; and adding yeast for making the white spirit in a semi-solid state to obtain the white spirit which has the antioxidant activity exceeding 80% compared with that of the existing white spirit and meets the national standard of semi-solid Luzhou-flavor white spirit. Compared with the traditional baijiu brewed by steaming sorghum, the total ester content of the baijiu brewed by adding the tannin enzymatic hydrolysate is increased by 87.50-102.27%, the free radical scavenging rate of DPPH is increased by 134.16-158.71%, and the content of guaiacol substances (4-ethyl guaiacol and guaiacol) is increased by 160.69-166.90%.

The present invention addresses the problem of improving the change in smell of stearyl glycyrrhetinate,. This composition is characterized by containing components (A)–(C). (A) Stearyl glycyrrhetinate, (B) niacinamide or tranexamic acid, and (C) at least one compound selected from the group that consists of anisyl alcohol, farnesol, eugenol, citronellol, α-pinene, 1-hexanol (hexen-1-ol), linalool, cedryl acetate, Iso E Super, Sandalore, Ambroxan, γ-terpinene, ambrettolide, β-ionone, borneol, isobutyl quinoline, citral, guaiacol, patchouli alcohol, cedrol, ethyl vanillin, maltol, linalyl acetate, α-terpineol, benzyl benzoate, damascenone, anisaldehyde, benzaldehyde, Magnolan, Mayol, phenylacetaldehyde dimethyl acetal, nerol, dimethyl benzyl carbinol, Bacdanol, methyl anthranilate, Muscenone, benzyl acetate, phenylethyl alcohol, phenylethyl acetate, and heliotropine.

The invention belongs to the technical field of microorganisms, and discloses bacillus licheniformis and application thereof. The strain is specifically bacillus licheniformis DY-1, is preserved in the China General Microbiological Culture Collection Center (CGMCC) on July 1, 2024, and has the preservation number of CGMCC No.31125. The strain is screened from fermented grains, belongs to a food safety level strain, and can produce amylase and various flavor substances. The strain and a fermentation product thereof can be used for preparing amylase; the production of one or a combination of more of acetoin, 2, 3-butanediol, 4-ethyl guaiacol, pyrazine and phenethyl alcohol can be selectively controlled by changing a fermentation substrate, and edible flavors with different high added values can be selectively obtained; the method can be applied to production of white spirit, produced amylase is beneficial to decomposition of starch in grain raw materials in the white spirit brewing process, produced flavor substances are beneficial to improvement of the overall flavor of the white spirit, and the method has good application prospects and practical value.

The present embodiments relate to mixtures, systems, kits, and methods useful to detect strains of Alicyclobacillus which express novel genetic variants of the vdcC gene. Alicyclobacillus strains which express these variants produce guaiacol which causes spoilage in fruit juice products. The methods and mixtures may be used to predict the spoilage potential of fruit juice products that are contaminated with Alicyclobacillus.

The invention discloses an eucommia ulmoides wood controllable carbonization-gradient hooping phenol fragrance barrel for white spirit, and belongs to the technical field of wine brewing equipment. In order to solve the problems that a traditional oak barrel is high in cost, an eucommia wood barrel is incomplete in pretreatment, uneven in structural stress and poor in flavor controllability, eucommia wood with the tree age of 18-22 years is adopted as a base material, and pretreatment of segmented steaming, microwave degreasing and gradient drying is conducted; the barrel body is provided with a controllable carbonization modification layer, a 304 stainless steel gradient hoop enhancement layer, a triple-closing sealing layer and a flow guide groove functional layer, and a composite fragrant juice activation process is matched. The barrel can dissolve active ingredients of eucommia ulmoides such as geniposide and aucubin, the leakage rate is smaller than or equal to 0.05%, the content of guaiacol is increased by 42 times compared with a traditional pottery jar, the cost is reduced by 60% compared with an imported oak barrel, and the barrel is suitable for high-quality ageing of Chinese high-degree white spirit.

The invention discloses Leishan fish sauce sour essence as well as a preparation method and application thereof. The leishan fish sauce acid essence is prepared from ethyl acetate, absolute ethyl alcohol, ethyl isovalerate, ethyl hexanoate, isoamyl alcohol, furfuryl alcohol, ethyl lactate, ethyl caprylate, acetic acid, diethyl succinate, methyl salicylate, ethyl benzoate, ethyl laurate, hexanoic acid, phenethyl alcohol, 4-methyl guaiacol, ethyl myristate, ethyl palmitate, ethyl oleate, capsicum oleoresin, ethyl phenylacetate and ginger oil. And lactic acid, glycerol triacetate, ocimene, ethyl isovalerate, ethyl butyrate, anethole and phenylacetaldehyde. The Leishan fish sauce sour essence disclosed by the invention is soft and harmonious in overall fragrance, natural and vivid, still has stable flavor characteristics at high temperature, can make up the problem of flavor deficiency in sour soup products, realizes accurate reproduction and controllable release of composite fragrance, improves the standardization level of products, and effectively improves the preference degree of consumers.

The present application belongs to the technical field of electronic atomization, and particularly relates to a tobacco extract composition, a preparation method and application thereof. The tobacco extract composition comprises 2,6-dimethylpyrazine, 3-acetylpyridine, dihydroactinidiolide, cembranoid diol, maackiain, furfuryl alcohol and guaiacol. The mass ratio of (2,6-dimethylpyrazine+3-acetylpyridine) to dihydroactinidiolide is greater than 0.02; the dihydroactinidiolide content is greater than or equal to 0.02 mg / g in terms of dry matter mass; and the guaiacol content is 0.004-0.025 mg / g. The overall roasting characteristics are sufficient, and the smoke-like feeling is strong. The preparation method comprises the following steps: first extracting tobacco raw materials to obtain extract A, low-temperature secondary extraction of the extract A to obtain tobacco flavor, high-temperature secondary extraction of the extract A to obtain smoke flavor, and mixing the tobacco flavor and the smoke flavor in a certain proportion to obtain the tobacco extract composition.

The present disclosure provides compositions, methods, and kits for detecting guaiacol-producing microorganisms, in particular heat resistant and acid resistant bacteria (TAB), in food. In one example, a method comprises: (a) contacting a set of oligonucleotides with a sample, where the set of oligonucleotides comprises: a first set of primers targeting a nucleic acid of a guaiacol-producing microorganism; and a second set of primers, the second set of primers being targeted to a nucleic acid of alicyclic bacillus acidoterrestris, the first set of primers being a nucleic acid of alicyclic bacillus acidoterrestris, and the second set of primers being a nucleic acid of alicyclic bacillus acidoterrestris. (b) amplifying the DNA in the sample with the set of oligonucleotides under a multiplex polymerase chain reaction (PCR); and (c) determining the presence of the PCR product of step (b), wherein the presence of the PCR product in the sample indicates that the sample is contaminated by both the guaiacol-producing microorganism and the Alicyclobacillus acidoterrestris.

This invention relates to a method for producing phenolic compounds by pyrolysis of lignin catalyzed by a double perovskite, comprising: preparation of a MOF precursor; calcination of the MOF precursor prepared in step one to prepare a MOF-based double perovskite AA' with tunable morphology, specific surface area, and pore structure. 1‑x K x B₂O₆, where A is a rare earth metal, A' is an alkaline earth metal, and B is a transition metal, the molar ratio of the metals in MOFs-based double perovskites is A:A':K:B = 1:(1- x ): x :2, where 0.01≤ x ≤0.40; MOFs-based double perovskite catalytic pyrolysis of lignin to produce phenolic compounds, the phenolic compounds in the liquid phase product include phenols, guaiacol, and syringol, with a total selectivity ≥65%; regeneration of double perovskite. This invention utilizes double perovskite catalytic pyrolysis of lignin to produce phenolic compounds, which can convert lignin into high-value-added chemicals.

The invention relates to the technical field of traditional Chinese medicine detection, in particular to a method for determining the content of various components in blumea balsamifera oil. According to the method, a gas chromatography-mass spectrometry (GC-MS) technology is adopted, and chromatographic conditions are as follows: a chromatographic column is an SH-Stabilwax (30m * 0.32 mm, and the film thickness is 0.25 [mu] m) capillary column; the temperature of a sample inlet is 230 DEG C; the split ratio is 10: 1; the sample size is 1 microliter; the mass spectrum conditions are as follows: the ion source temperature is 200 DEG C; the interface temperature is 230 DEG C; the ionization mode is electron impact ion (EI); the detection voltage is 0.2 KV; the solvent is delayed for 2 minutes; the mass number scanning range is 30 to 400 amu; and SIM mode. According to the method disclosed by the invention, the contents of alpha-pinene, camphene, beta-pinene, limonene, p-cymene, camphor, alpha-coumarin, linalool, bornyl acetate, beta-caryophyllene, myrtenal, alpha-caryophyllene, L-borneol, perillaldehyde, caryophyllene oxide, guaiacol, beta-eudesmol and xanthoxylin in the blumea balsamifera oil can be simultaneously determined; the method provided by the invention has good precision, repeatability and stability, and the quality consistency of the blumea balsamifera oil can be quickly, accurately and effectively evaluated through detection of multiple components.

The invention belongs to the technical field of daily chemicals, and particularly relates to Chinese herbal medicine three-color strip toothpaste and a preparation method thereof. The Chinese herbal medicine three-color strip toothpaste comprises a faint yellow paste body, a green paste body and a blue paste body, the faint yellow paste body comprises, by mass, 0.1%-2% of chitosan quaternary ammonium salt and 0.1%-1% of an ovateleaf holly bark extract, and the green paste body comprises, by mass, 0.002%-0.15% of sodium copper chlorophyllin and 0.1%-1% of the ovateleaf holly bark extract. The blue paste is prepared from the following components in percentage by mass: 0.03 to 0.15 percent of guaiazulene sodium sulfonate and 0.1 to 1 percent of ovateleaf holly bark extract. According to the Chinese herbal medicine three-color-strip toothpaste and the preparation method thereof provided by the invention, the three-color-strip toothpaste is obtained under the condition that exogenous pigments are not added; the toothpaste can achieve the compound effects of freshening breath, inhibiting dental plaque and oral pathogenic bacteria, resisting inflammation, diminishing swelling, resisting allergy, relieving gingivitis and promoting healing and repairing of recurrent oral ulceration, can promote enamel remineralization and pore repairing, and is high in effect synergy, paste stability and use safety.

The application discloses a natural tobacco flavor characteristic spice suitable for low-temperature cigarettes, and has the following weight parts: a smoking agent 40-70, a taste improver 5-20, and a tobacco flavor characteristic spice base 10-30; wherein the tobacco flavor characteristic spice base is composed of extracts of medlar, sea buckthorn and hyssop, and further comprises 2-acetylpyrazine, 2,3-dimethylpyrazine, 2,3,5-trimethylpyrazine, beta-damascenone and guaiacol as main spices, and further comprises a blending agent, a fixative, a flavor enhancer and a diluent; the natural tobacco flavor characteristic spice suitable for low-temperature cigarettes and the preparation method have the following advantages: different natural extracts, such as medlar, sea buckthorn and hyssop, can be used to provide unique tobacco flavors for cigarettes, and can increase the sour and sweet aroma of low-temperature cigarettes, so that the low-temperature cigarettes are more in line with the taste preferences of consumers; other auxiliary spices and chemicals, such as the blending agent, the fixative, the flavor enhancer and the diluent, are added to provide more rich levels and tastes.

The invention discloses dark baked barley rich in wheat fragrance as well as a preparation method and application of the dark baked barley, and belongs to the technical field of wheat making. Comprising the following steps: selecting barley of which the basic amino acid content is more than or equal to 30mg / L and the lignin content is less than or equal to 100mg / g as The barley baking method is characterized in that barley is baked in a sectional manner, and the barley baking method comprises the following steps: in the first stage, the barley is heated to 50-55 DEG C at a constant speed, and the water content of the barley is 12-15%; in the second stage, the temperature is rapidly increased to 90 DEG C, and the water content of the barley is reduced to 8-10%; in the third stage, the baking temperature continues to be increased to 210-220 DEG C, and baking is conducted for 20-30 min; in the fourth stage, when the crispness of the baked barley reaches 98% or above, the baking temperature is lowered to 190-210 DEG C, and then the baked barley is rapidly cooled to the room temperature. The preparation method is applied to the aspect of drinks, and solves the problems that although the color of the baked barley obtained by the existing dark-color baked barley preparation process meets the requirement, the barley fragrance cannot be highlighted due to the low pyrazine content, the smoky flavor is large due to the high guaiacol content, and the technical requirement that pure and rich barley fragrance can be obtained by extracting a small amount of the baked barley cannot be met.

The present application belongs to the technical field of electronic atomization, and particularly relates to a tobacco extract composition, a preparation method and application thereof. The tobacco extract composition comprises methylcyclopentenolone, guaiacol, neophytadiene, cedrene diol, dihydroactinidiolide, maltol, furaneol, palmitic acid, 6-ethyl-5,6-dihydro-2H-pyran-2-one and scopoletin; the range of (methylcyclopentenolone+guaiacol) / neophytadiene is 0.01-2.5 by mass; the guaiacol content is 0.003-0.02 mg / g by dry matter mass; the tobacco roasting aroma is richer, the tobacco roasting sweet note is more obvious, and the tobacco-like feeling is better. The preparation method comprises: extracting tobacco raw materials twice at medium and low temperatures to obtain tobacco flavor substances, extracting the tobacco raw materials at high temperature to obtain smoke flavor substances, and mixing the tobacco flavor substances and the smoke flavor substances at a certain ratio to obtain the tobacco extract composition.

The present disclosure relates to methods, compositions, and kits for determining whether a liquid contains a guaiacol-producing bacterium. In embodiments, a method comprises the steps of (a) mixing a media that changes color with a change in pH with a volume of a liquid that is potentially contaminated with one or more guaiacol-producing bacteria to create a sample, (b) placing the sample in a sterile container, (c) incubating the sample in the sterile container at about 30°C to about 60°C, (d) determining whether the sample has undergone a color change, an odor change, and / or a change in clarity following step (c), and (e) determining whether the liquid contains a guaiacol-producing bacteria based upon the determination in step (d). In embodiments, the liquid that is potentially contaminated with one or more guaiacol-producing bacteria is an acidic fruit juice. In embodiments, the guaiacol-producing bacteria are guaiacol-positive thermophilic acidophilic bacteria.

The application relates to a method for preparing natural gas by taking biomass and polyhydroxy compounds as raw materials. The method comprises the following steps: mixing the raw materials with water and a catalyst, and carrying out a reduction reaction in a reducing atmosphere to obtain a product containing natural gas; the raw materials are selected from biomass raw materials or / and polyhydroxy compounds; the biomass raw materials are selected from at least one of beech sawdust, poplar sawdust, pine sawdust, birch sawdust, wheat straw or corn straw; the polyhydroxy compounds are selected from at least one of cellulose, hemicellulose, cellobiose, xylose, guaiacol, sorbitol, isosorbide, glycerol, erythritol, xylitol or glucose; the catalyst comprises a carrier and an active component loaded on the surface of the carrier; the active component is selected from metal elements; the metal elements are selected from at least one of the single elements of ruthenium, rhodium, platinum, palladium or iridium. The method provides a path for converting biomass such as sawdust and polyhydroxy compounds to prepare natural gas, and has important industrial application prospects.

The invention provides oak chips for ageing high-quality beer as well as a preparation method and application of the oak chips. Every 100 g of the oak sheet is prepared from the following components by mass: 1600 to 15000 mg of vanillin, 750 to 1600 mg of whisky lactone, 4500 to 22500 mg of furfural, 25 to 80 mg of guaiacol, and 55 to 70 mg of eugenol. The preparation method of the oak chips for aging beer comprises the steps of baking treatment and ultraviolet photolysis treatment of raw oak chips, the temperature of the baking treatment is 150-240 DEG C, and the optical power density of the ultraviolet photolysis treatment is 0.6-0.8 W / m < 2 >. The oak chips disclosed by the invention are high in content of phenols and lactones, and high-quality beer which is excellent in balance degree and harmony of various fragrances can be obtained in a short time.

This invention belongs to the field of atomization technology, specifically relating to a coffee extract, an atomizing matrix, and an electronic atomization device. The coffee extract provided by this invention, by mass fraction, comprises 350-450 parts furfuryl alcohol, 5-10 parts furfuryl formate, 2-5 parts 2-acetylfuran, 5-10 parts 5-methylfurfural, 20-30 parts furfuryl acetate, 10-20 parts guaiacol, 30-40 parts maltol, 5-15 parts 4-ethylguaiacol, 5-15 parts difurfuryl ether, and 10-20 parts 4-vinylguaiacol. The coffee extract provided by this invention has a low caffeine content but a strong characteristic coffee aroma; when applied to an atomizing matrix, it can enhance the taste and inhalation experience of the aerosol.

The invention belongs to the technical field of enzyme activity determination. The combined determination method comprises the following steps: (1) adding plant tissues into liquid nitrogen, grinding, adding a phosphate buffer solution with the pH value of 7.8, continuously grinding until no crude fiber exists, and centrifuging to obtain an enzyme extracting solution; (2) determining the activity of SOD in the enzyme extracting solution by adopting a nitrogen blue tetrazole photochemical reduction method; (3) preparing a reaction system by using a phosphate buffer solution with the concentration of 50mmol. L <-1 > and the pH value of 5.5, and determining the POD activity in the enzyme extracting solution by adopting a guaiacol colorimetric method; and (4) determining by adopting an ultraviolet spectrophotometer method, immediately starting timing after H2O2 is added, reading once every 1min until the OD value is unchanged, and calculating the CAT activity by using the average value of OD change per minute. The method can solve the problems of tedious experimental steps, low efficiency, unstable data and the like due to respective extraction of three enzymes, ensures the accuracy of enzyme activity determination, simplifies the experimental steps, and improves the experimental efficiency and the data stability.

To provide a coffee extract with increased coffee-specific aroma and reduced off-flavors.SOLUTION: A coffee extract comprises 2,3-butanedione, guaiacol and 4-ethylguaiacol. In the coffee extract, the ratio of (a) the concentration (ppb) of 2,3-butanedione to (b) the total concentration (ppb) of guaiacol and 4-ethylguaiacol [(a) / (b)] is 3.1 or more, and the coffee solid content (Brix) is 20-40%.SELECTED DRAWING: None

The present application provides a kind of marine bacteria capable of degrading lignin and removing nitrite, which is a deep-sea seabed sulfide source, and has the function of degrading lignin and removing nitrite.The strain of Halomonas sp.has a preservation number of CGMCC No.34690.The strain screened in the present application can grow and reproduce in seawater, can utilize guaiacol as a carbon source, and can remove nitrite.The strain of the present application has laccase activity, has laccase gene and nitrite reductase gene.The experimental results show that the cumulative removal rate of guaiacol after 2 days of culture is 56%, and the removal rate of nitrite after 4 days of culture is close to 70%, which has great application potential in the marine aquaculture industry.