264 results about "DPPH" patented technology

The invention discloses bacillus velezensis as well as fermentation product filtrate and a biological product thereof, and belongs to the technical field of microorganisms, a bacillus velezensis BTN-HB-F4 strain is separated and purified from prinsepia utilis royle in Harba snow mountain in Yunnan province, the fermentation product filtrate of the bacillus velezensis has excellent HAS inhibition capacity and remarkable DPPH and ABTS free radical scavenging capacity, and the biological product of the bacillus velezensis has the advantages that the biological product can be used for preparing biological products of the prinsepia utilis royle; a good anti-oxidation effect can be realized; secondly, under various concentrations, fermentation product filtrate can remarkably reduce the proportion of SA-beta-Gal positive cells, gene expression of matrix metalloproteinase 3 can be remarkably inhibited, and a good anti-aging effect is achieved; in addition, fermentation product filtrate can remarkably inhibit generation of zebra fish melanin and has a good whitening effect; the secretion amount of cell inflammatory factors IL-6 and TNF-alpha can be remarkably inhibited, and a good relieving effect is achieved; finally, the fermentation product filtrate can effectively inhibit the growth of pathogenic bacteria staphylococcus aureus, and has the potential of regulating the skin micro-ecology.

The invention relates to polygonum multiflorum exosome-like vesicles and application thereof, the polygonum multiflorum exosome-like vesicles are applied to preparation of antioxidant products, anti-inflammatory products and anti-ultraviolet products, the antioxidant products are products for removing one or more of DPPH free radicals, ABTS free radicals and hydroxyl free radicals, the anti-inflammatory products are products for inhibiting hyaluronidase, and the anti-ultraviolet products are products for removing one or more of DPPH free radicals, ABTS free radicals and hydroxyl free radicals. And the anti-ultraviolet product is a product capable of absorbing UVB. Compared with the prior art, the polygonum multiflorum exosome-like vesicles prepared by the method disclosed by the invention are low in cost and large in treatment capacity, and the obtained polygonum multiflorum exosome-like vesicles have excellent oxidation resistance, inflammation resistance and ultraviolet resistance.

The present invention provides a novel antioxidant Lactiplantibacillus paracasei TDM-2, its cell-free extract and applications, belonging to the technical field of lactic acid bacteria. The Lactiplantibacillus paracasei TDM-2 provided by the present invention is deposited in the China General Microbiological Culture Collection Center, located at No. 3, Yard 1, Beichen West Road, Chaoyang District, Beijing. The deposit date is February 20, 2023, and the deposit number is CGMCC NO. 26579. The Lactiplantibacillus paracasei TDM-2 and its cell-free extract provided by the present invention have good scavenging rates of DPPH free radicals and tolerance to H2O2, and also have good reducing ability and scavenging ability for OH ‑ ions.

The invention discloses a Pediococcus pentosaceus strain and an application thereof. Belongs to the technical field of functional component conversion. According to the content of total saponin and polysaccharide in American ginseng, a lactic acid bacterium capable of efficiently converting saponin and polysaccharide is screened by taking a comprehensive score as an index, is identified as pediococcus pentosaceus through morphology, physiology, biochemistry and molecular biology and is preserved in the China General Microbiological Culture Collection Center (CGMCC), and the preservation number is CGMCC No.33430. The contents of total saponins in the transformed American ginseng and ginseng are respectively increased by 53.90% and 209.61%, and the contents of polysaccharides are respectively increased by 124.68% and 68.16%. The DPPH antioxidant capacity, the ABTS antioxidant capacity, the blood sugar reducing capacity and the blood fat reducing capacity of the converted American ginseng and ginseng total saponin extracting solution and the polysaccharide extracting solution are all improved. The pediococcus pentosaceus obtained by screening has a wide application prospect.

The invention belongs to the technical field of plant fermentation, and relates to fructus arctii fermentation liquor with soothing and anti-inflammatory effects as well as a preparation method and application of the fructus arctii fermentation liquor. The fructus arctii fermentation liquid is obtained by fermenting fructus arctii through schizosaccharomyces pombe JM2-16, and the fructus arctii fermentation liquid is obtained by fermenting fructus arctii through schizosaccharomyces pombe JM2-16. According to the invention, after the fructus arctii is used as a raw material and is fermented by the schizosaccharomyces pombe, the hyaluronidase inhibition capability, the elastase inhibition capability and the DPPH free radical scavenging capability of the fermentation product can be improved, so that the fermentation product with better soothing, anti-inflammatory and anti-oxidation capabilities is obtained, and the requirements of the market on efficient, natural and safe skin care components are met.

The invention discloses a Bacillus velez, a fermentation product filtrate thereof and a biological product thereof, belonging to the field of microbial technology. A Bacillus velez BTN-HB-F4 strain is obtained by isolating and purifying green prunes from Haba Snow Mountain in Yunnan Province. The fermentation product filtrate thereof has excellent HAS inhibition ability and significant DPPH and ABTS free radical scavenging ability, and can achieve good antioxidant effect. Secondly, at various concentrations, the fermentation product filtrate thereof can significantly reduce the proportion of SA-β-Gal-positive cells, can significantly inhibit the gene expression of matrix metalloproteinase 3, and has good anti-aging effect. In addition, the fermentation product filtrate thereof can significantly inhibit the production of zebrafish melanin, and has good whitening effect. The fermentation product filtrate can significantly inhibit the secretion of cellular inflammatory factors IL-6 and TNF-α, and has good soothing effect. Finally, the fermentation product filtrate thereof can also effectively inhibit the growth of pathogenic bacteria Staphylococcus aureus, and has the potential to regulate skin microecology.

The invention belongs to the field of biological medicines, and particularly relates to a preparation method of a pomegranate peel extract. The method comprises the following steps: pretreating fresh pomegranate rind, and extracting with mixed alcohol solvents with different concentrations. According to the extraction method provided by the invention, the pomegranate rind can be fully swelled after being soaked in the alginate oligosaccharide aqueous solution, and active ingredients in the pomegranate rind can be fully extracted after subsequent treatment, so that the extraction efficiency is improved, and the solubility and the stability of the active ingredients are improved; according to the preparation method, the mixed alcohol solvent is added in a stepped manner, so that the extraction rate of active ingredients is increased, and meanwhile, the prepared extract is increased in removal rate of DPPH free radicals and has excellent oxidation resistance.

The invention discloses a strain of Blastococcus atamensis YC-1 and application of the strain of Blastococcus atamensis YC-1, and belongs to the field of microbial strains. The streptococcus sp. YC-1 provided by the invention has high antioxidant activity and also has the capability of producing DHHB (docosahexaenoic acid); meanwhile, the YC-1FLE is prepared by adopting absolute ethyl alcohol as a solvent, has no toxicity to HaCaT cells, is relatively high in reducing capacity, has a scavenging effect on DPPH free radicals, ABTS cation free radicals and hydroxyl free radicals, has a tyrosinase inhibition effect and shows good antioxidant and whitening effects; when the YC-1FLE is applied to the sunscreen lotion, the sunscreen coefficient of the sunscreen lotion containing the YC-1FLE is obviously higher than that of a blank control group, which shows that the YC-1FLE has better ultraviolet resistance. Therefore, the strain and the lysate extract of the fermentation product of the strain have huge application potential in the field of cosmetics.

The invention discloses a corn active peptide with effects of dispelling effects of alcohol, reducing uric acid and resisting oxidation, a preparation and application of the corn active peptide, and belongs to the technical field of biological active peptides. The amino acid sequence of the corn active peptide is at least one of LMFP, FEGLFR, FLR and QLPSYR. The corn active peptide with the effects of dispelling the effects of alcohol, reducing uric acid and resisting oxidation is separated from corn germ meal through enzymolysis, ultrafiltration and other technologies, amino acid sequence determination is conducted on the separated peptide fragments, screening is conducted in combination with the bioinformatics technology, and finally four peptide fragments are obtained. According to the present invention, the corn active peptides are identified, chemical synthesis is performed according to the identified peptide fragment sequence, the effects of alcohol effect dispelling, uric acid reducing and oxidation resistance of the synthesized single peptide fragment and the compounded peptide fragment are determined, and the corn active peptides have good XOD inhibition rate, good DPPH clearance rate and good ADH activation rate, and have important application prospects in the development of the products with effects of alcohol effect dispelling, uric acid reducing and oxidation resistance.

The invention discloses a gelatinized and / or micronized nucleoside drug and a preparation method thereof, and belongs to the technical field of drugs. The preparation method comprises the following steps: mixing lipoic acid, a nucleoside drug and an esterification catalyst, carrying out a grafting reaction in a reaction solvent to obtain a lipoic acid grafted nucleoside drug, dispersing the lipoic acid grafted nucleoside drug in an organic solvent to obtain a dispersion liquid, dropwise adding the dispersion liquid into hot water, and carrying out ring opening polymerization to obtain the lipoic acid grafted nucleoside drug. The nucleoside drug nanogel, nanoparticle or nanofiber is obtained, and the structure and performance improvement based on the drug itself is realized. The gelated and / or micronized nucleoside medicine prepared by the invention has obviously improved scavenging rates of DPPH free radicals and ABTS free radicals, and the method can improve the oxidation resistance of the nucleoside medicine and has the characteristic of improving the stability and functionality of the medicine.

The invention discloses a method for increasing the extraction yield of tremella polysaccharide and application, dry tremella is used as a raw material, tremella polysaccharide is effectively extracted by utilizing an ultrasonic-cellulase wall breaking coupling green solvent method, the extraction yield of tremella polysaccharide is effectively increased, the method is green and environment-friendly and is easy to control, and the obtained tremella polysaccharide has excellent biological activity and can be used for preparing the tremella polysaccharide. The water loss rate of water can be reduced, and the moisturizing and anti-wrinkle capabilities are good; the caenorhabditis elegans can effectively remove hydroxyl free radicals, DPPH free radicals and ABTS free radicals, shows strong oxidation resistance, can significantly prolong the survival rate of caenorhabditis elegans under hydrogen peroxide stress, and shows that the caenorhabditis elegans has good oxidative stress resistance.

The invention provides application of an immature bitter orange extract in preparation of anti-oxidation and anti-inflammatory drugs. The fructus aurantii immaturus is extracted by using different solvents, and the extract is researched, so that the fructus aurantii immaturus extract has the capability of removing superoxide anion free radicals, ABTS free radicals and DPPH free radicals; in addition, the immature bitter orange extract can also reduce the content of nitric oxide, tumor necrosis factor-alpha, interleukin-1beta and interleukin-6 released by LPS-induced macrophages. The fructus aurantii immaturus extract has anti-oxidation and anti-inflammatory effects, and is used as an anti-oxidation and anti-inflammatory drug to develop a new application of an old drug of the fructus aurantii immaturus extract.

The invention discloses a broad-spectrum antibacterial photo-thermal biological film as well as a preparation method and application thereof, and belongs to the technical field of daily chemistry and food science. Keratin and carboxymethyl cellulose are used as raw materials, phenolated lignin nanoparticles are used as an antibacterial agent, and the prepared biological film shows excellent mechanical toughness (elongation at break gt; 150%) and self-adhesion, can form a tight package after simple pressing, has rapid photoresponse ability, can be heated to 50 DEG C or above within 1 minute, has broad-spectrum photothermal antibacterial ability and antioxidant ability, has a staphylococcus aureus and escherichia coli inhibition rate of more than 90%, and has a DPPH free radical scavenging rate gt; and the fresh-keeping period of fresh vegetables and fruits can be effectively prolonged. The prepared biological film can replace a plastic film to be applied to packaging of foods such as vegetables, fruits and grains, loss is reduced while plastic pollution is reduced, and a solution is provided for construction of a sustainable packaging system.

The invention discloses low-molecular-weight enteromorpha polysaccharide with antioxidant activity and a preparation method of the low-molecular-weight enteromorpha polysaccharide, and belongs to the technical field of polysaccharide preparation. The preparation method comprises the following steps: taking an enteromorpha crude polysaccharide solution, adding H2O2 to enable the concentration of the enteromorpha crude polysaccharide solution to be 0.04-0.08 M, and degrading for 90-120 minutes under ultraviolet rays to obtain the low-molecular-weight enteromorpha polysaccharide, the low-molecular-weight enteromorpha polysaccharide is composed of monosaccharides of rhamnose, glucose, galactose, xylose, mannose and arabinose according to the ratio of 1.08: 1.00: 0.83: 0.58: 0.69: 0.06, the scavenging capacity and the iron reducing capacity of DPPH, ABTS and hydroxyl free radicals are improved, and the low-molecular-weight enteromorpha polysaccharide is obtained. The compound has excellent antioxidant activity and can be used as or used for preparing an antioxidant active preparation. The preparation method disclosed by the invention is efficient and stable, can be used for preparing the low-molecular-weight enteromorpha polysaccharide with lighter color, lower molecular weight and higher antioxidant activity, and has higher potential application value.

The invention discloses nano-enzyme coating recombinant probiotics as well as a preparation method and application thereof, and belongs to the technical field of biotechnology and nano medicine. According to the invention, engineered Escherichia coli Nissle 1917 (ECN (DE3)) is taken as a host, and glutathione peroxidase (GPX) and glutathione reductase (GR) genes are over-expressed through a genetic engineering means, so that an efficient antioxidant enzyme system is constructed. And sequentially modifying chitosan and cerium dioxide nano-enzyme (CNP) on the surfaces of the thalli by using an electrostatic layer-by-layer self-assembly technology to form a protective coating. The nano-enzyme coating significantly improves the survival rate of the recombinant probiotics in severe environments such as simulated gastric acid (pH 2.5) and bile salt (0.3%), and enhances the ability of the recombinant probiotics to remove reactive oxygen species (ROS), including hydrogen peroxide, superoxide anions, hydroxyl free radicals and DPPH free radicals.

The invention discloses a catechol ether compound as well as a pharmaceutical composition and application thereof. The structure of the compound is as shown in a formula I, the compound further comprises pharmaceutically acceptable salt of the compound, the compound has PDE4 inhibition and antioxidation dual functions, has inhibition and removal effects on PDE4 and free radical DPPH at the same time at the micromolar concentration level, shows anti-inflammatory and antioxidative stress dual effects in vivo and in vitro, and can be used for preparing a medicine for preventing and treating PDE4 and free radical DPPH. Therefore, the compound has a wide application prospect in the aspect of treating diseases related to inflammation and oxidative stress. # imgabs0 #

The invention relates to glassine ester as well as a preparation method and application thereof. The structural formula of the glassine ester is shown as a formula (I), each R1 is independently selected from hydrogen and-C (= O) R, and at least one R1 is-C (= O) R; r is selected from C7-C21 alkyl groups and C7-C21 unsaturated chain alkyl groups. The glassine ester has a remarkable DPPH free radical scavenging effect, can remarkably improve expression of elastin, hyaluronic acid and I-type collagen in cells, and has excellent effects of oxidation resistance, moisturizing and / or tightening and wrinkle resistance when being added into cosmetics as a functional component. # imgabs0 #

The invention provides application of epiphyllum callus extract in preparation of cosmetics, and belongs to the technical field of new biological materials. Compared with a common epiphyllum extract, the epiphyllum callus extract provided by the invention is rich in high-content active ingredients, such as kaempferol, general flavone, total polyphenol, total protein and total sugar; the antioxidant skincare effect is achieved, and the removal efficiency of cells on DPPH free radicals can be improved. The skin care effect of promoting ATP synthesis is achieved, ATP synthesis can be remarkably promoted, and energy metabolism of damaged mitochondria is improved. Effective components of epiphyllum calluses are extracted, the effect of the epiphyllum calluses on human immortal keratinocytes is evaluated through cell experiments, it is proved that the epiphyllum calluses have excellent effects of resisting oxidation, resisting inflammation and repairing the keratinocytes, and the epiphyllum calluses are suitable for being used as raw materials to be applied to the cosmetic industry.

The invention discloses a preparation method and application of a blueberry anthocyanin extract, and belongs to the technical field of natural product extraction. The preparation method comprises the following steps: mixing blueberry pomace powder and water according to a solid-liquid ratio of 1: (10-50), exhausting, performing ultrasonic-assisted extraction, adding hemicellulase accounting for 0.6-1.4% of the mass of the pomace powder, extracting at 40-60 DEG C for 1-4 hours, and finally centrifuging to obtain a blueberry anthocyanin extracting solution. According to the method, the blueberry pomace is cooperatively treated by ultrasonic waves and hemicellulase, so that the defects of low efficiency and easy degradation of anthocyanin in a traditional extraction method are overcome, and the method has the outstanding advantages of high extraction rate, good activity maintenance and green and environment-friendly process. The obtained extract shows excellent DPPH and ABTS < + > free radical scavenging ability, and can be widely applied to functional food, health care products and cosmetics as a natural antioxidant.

The invention discloses an antioxidant repairing composite composition containing a picea norvegensis leaf extract and a preparation method thereof, and relates to the field of skincare products, the picea norvegensis leaf extract, leontopodium alpinum and citrus fruit extract are used as main materials, and the picea norvegensis leaf extract and leontopodium alpinum can play a role in the whole pathway of Nrf2 and NF-kappa B, so that the antioxidant repairing composite composition has the advantages that the antioxidant repairing composite composition is free of toxic and side effects; and the citrus fruit extract can inhibit the activation of TRPV1 and relieve the discomfort of the sensitive skin, so that the composition not only can improve the skin barrier of the sensitive skin, but also can inhibit the activity of the TRPV1, and the TRPV1 can be activated only by breaking the barrier and damaging the barrier under the two actions, so that the skin barrier can be well repaired. And the vicious circle of barrier injury can be aggravated by the excessive activation of the TRPV1, so that the stabilization and repair of the skin are realized, and the emulsion provided by the invention has a relatively good DPPH clearance rate and relatively good oxidation resistance.

The invention belongs to the technical field of fermentation and metagen preparations, and particularly relates to application of bifidobacterium breve metagen in the aspects of scavenging free radicals and relieving oxidative aging. According to the present invention, the oxidative free radical scavenging experiment results show that the bifidobacterium breve NKU BB 1-2 metagen has good oxidative free radical scavenging ability, and has good scavenging ability on hydroxyl free radicals, superoxide anions and DPPH free radicals; meanwhile, through component analysis, it is found that the bifidobacterium breve NKU BB 1-2 metagen contains various antioxidant and anti-aging bioactive substances such as flavonoids, indoles, amino acids, bile acids and ceramides; a cell model experiment verifies that the bifidobacterium breve NKU BB 1-2 metagen can obviously relieve oxidative damage to the cells and improve the oxidation resistance of the cells, so that oxidative aging is relieved.

The invention provides a composition containing exosome, which is prepared from the following components in parts by weight: 10 to 20 parts of exosome, 5 to 15 parts of extract, 0.3 to 0.5 part of antioxidant and 0.05 to 0.1 part of preservative, the extract is a mixture of 3-8 parts of aloe extract, 1-5 parts of camellia flower extract and 1-3 parts of cranberry extract; the preservative is one or more of benzoic acid, salicylic acid, boric acid and sorbic acid; the antioxidant is propyl gallate. When the composition is applied to an anti-aging product, the temperature sensing effect and the DPPH free radical scavenging rate are obviously improved.

The application discloses a kind of earthworm protein with twelve 3 peptides, which is prepared by degrading earthworm protein with catalytic amount of Pro-Ala-Lys. The application also discloses the preparation method of the earthworm protein with twelve 3 peptides and the application of the earthworm protein with twelve 3 peptides in removing ·OH free radicals and DPPH free radicals. Experimental results show that the earthworm protein with twelve 3 peptides has excellent effects in removing ·OH free radicals and DPPH free radicals. Therefore, the earthworm protein with twelve 3 peptides provides an effective technical means for preparing ·OH free radical scavenger and DPPH free radical scavenger.

The present invention belongs to the field of cosmetics and discloses a composition, which includes prickly ash fruit extract, epigallocatechin gallate, diacetylboldine, sanguisorba root extract, and niacinamide. The composition has excellent antioxidant efficacy as detected by the DPPH method. At the same time, the present invention also discloses a composition for resisting heat stimulation and UV stimulation. By using a nematode model for detection, it can significantly inhibit the level of lipofuscin generation in nematodes after heat stimulation and ultraviolet light stimulation, and there is a significant synergistic effect on the inhibition of lipofuscin generation in nematodes after heat stimulation and ultraviolet light stimulation when the composition is used in combination with withania somnifera root extract. It shows that the composition of the present invention has very good anti-wrinkle effects of anti-photoaging and anti-thermal aging, and can effectively prevent the formation of skin spots.

The invention discloses a method for adjusting functional properties and oxidation resistance of potato protein, and relates to the technical field of food processing. The method comprises the following steps: extracting potato protein by adopting an alkali-solution and acid-isolation method, and modifying the potato protein by utilizing an ultrasonic technology; the ultrasonic frequency is set to be 20 kHz, the amplitude is set to be 40%, the ultrasonic time is 0-10 min, and the change rule of the potato protein structure and the functional property under different ultrasonic time is analyzed. After ultrasonic treatment, the particle size and polydispersity index of the potato protein are obviously reduced, the Zeta-potential is increased, but main strips are not changed; after 6 minutes of ultrasonic treatment, the particle size is 266.90 + / -2.26 nm, the PDI is 0.27 + / -0.03, and the Zeta-potential is-26.25 + / -0.93 mV. After 6 minutes of ultrasonic treatment, the solubility of potato protein is the highest, the foamability is improved by 1.18 times, the emulsifying activity is improved by 1.54 times, and the digestibility of simulated intestines is remarkably improved. Under the condition, the reducing power of the potato protein and the scavenging rates of DPPH, superoxide anions and hydroxyl radicals reach the maximum value.

The preparation method of the camellia oil fermentation product comprises the following steps that a fermentation base solution containing camellia oil and a strain seed solution are mixed, the strain seed solution comprises at least one of lactobacillus plantarum and saccharomyces cerevisiae, fermentation culture is conducted, and the camellia oil fermentation product is obtained. The fermentation liquor containing the camellia oil leavening is obtained. Compared with a camellia oil fermentation product obtained by fermenting other strains, the camellia oil fermentation product provided by the scheme has higher antioxidant activity (DPPH and ABTS free radical scavenging ability), has stronger ability of inhibiting cells from spontaneously generating NO (nitric oxide), shows good biocompatibility in a cell experiment, and can be used for preparing a camellia oil fermentation product. The obvious adverse effect on the cell activity is not generated.

The invention discloses a small molecule peptide with antioxidant activity as well as a preparation method and application of the small molecule peptide. According to the technical scheme, the method comprises the following steps: 1) carrying out enzymolysis on squid viscera by adopting neutral protease (4000U / g, pH 7.0, 50 DEG C and 8 hours), carrying out freeze drying, and identifying peptide fragments through MALDI-TOF mass spectrometry; 2) binding molecular docking (AutoDock Vina screening binding energy lt; the method comprises the following steps of: screening out two small molecular peptides YVPGKFRQTN and GLPQTMEVYLR with antioxidant activity (-8.0 kcal / mol) and molecular dynamics simulation verification, namely YVPGKFRQTN and GLPQTMEVYLR with antioxidant activity; and 3) experimental verification shows that the DPPH free radical scavenging rates of the two are respectively 89.4% and 83.1% when the concentration of the two is 2.5 mg / mL, and oxidative stress is remarkably inhibited by up-regulating an Nrf2 pathway (protein expression is 3.0-3.5 times, and HO-1 / SOD1 gene expression is 3.6-4.1 times). The obtained antioxidant activity small molecule peptide can be applied to development of functional food, cosmetics and anti-aging drugs, and a new way is provided for high-value utilization of squid byproducts.

The application discloses a South China Sea Decapterus maruadsi polypeptide with uric acid reducing and antioxidant activities and an application thereof, and relates to the technical field of biology. The polypeptide with high uric acid reducing activity and antioxidant activity is screened through activity prediction and molecular docking, and the amino acid sequence is FYDWWK. The polypeptide can be stably combined with the active center of xanthine oxidase, forms multiple hydrogen bonds and hydrophobic interaction, significantly inhibits the generation of uric acid, and has an in-vitro xanthine oxidase inhibition IC 50 of 3.068±0.2547 mmol / L. Meanwhile, the polypeptide has excellent DPPH free radical scavenging capacity, and an IC 50 of 5.033±0.5476 mmol / L. The polypeptide has good stability, still has high efficient inhibitory activity after simulated gastrointestinal digestion, and has oral development potential. The polypeptide is a natural marine small molecule active peptide, is safe and easy to absorb, and can be applied to the preparation of products related to uric acid reducing and / or antioxidant.

The invention belongs to the technical field of biology, and particularly relates to acetylated raffinose and application thereof. According to the invention, acetylation modification is carried out on raffinose by using an acetylation reagent to obtain acetylated raffinose, the acetylated raffinose can significantly improve the scavenging ability and total antioxidant ability of raffinose on DPPH free radicals, and the scavenging ability on hydroxyl free radicals and the reduction ability on iron ions are also improved to a certain extent. The acetylated raffinose provided by the invention has potential application value in development of functional foods and antioxidants.

The invention discloses an antibacterial and antioxidant sodium alginate film as well as a preparation method and application thereof, and belongs to the technical field of antibacterial materials. Epsilon-polylysine and tannic acid are synergistically compatible and are loaded on the surface of a sodium alginate matrix by adopting an atomization spraying technology, so that an antibacterial and antioxidant sodium alginate film is formed. The membrane successfully integrates the broad-spectrum antibacterial property of EPL and the strong antioxidant activity of TA, solves the problem of single function in the prior art, and realizes the synergistic interaction of antibacterial property and antioxidant property; secondly, due to the stable combination and slow release characteristics between TA and sodium alginate molecules, the membrane shows remarkable and lasting functionality, for example, the clearance rate of the obtained SA / EPL / TA membrane to DPPH free radicals can still be kept at 90% or above 7 days later, and the DPPH free radicals can be kept at 90% or above. The defect of short efficacy caused by volatilization or rapid migration of a traditional film added with a small-molecule antioxidant (such as plant essential oil) is effectively overcome.