316 results about "DPPH" patented technology

The invention discloses bacillus velezensis as well as fermentation product filtrate and a biological product thereof, and belongs to the technical field of microorganisms, a bacillus velezensis BTN-HB-F4 strain is separated and purified from prinsepia utilis royle in Harba snow mountain in Yunnan province, the fermentation product filtrate of the bacillus velezensis has excellent HAS inhibition capacity and remarkable DPPH and ABTS free radical scavenging capacity, and the biological product of the bacillus velezensis has the advantages that the biological product can be used for preparing biological products of the prinsepia utilis royle; a good anti-oxidation effect can be realized; secondly, under various concentrations, fermentation product filtrate can remarkably reduce the proportion of SA-beta-Gal positive cells, gene expression of matrix metalloproteinase 3 can be remarkably inhibited, and a good anti-aging effect is achieved; in addition, fermentation product filtrate can remarkably inhibit generation of zebra fish melanin and has a good whitening effect; the secretion amount of cell inflammatory factors IL-6 and TNF-alpha can be remarkably inhibited, and a good relieving effect is achieved; finally, the fermentation product filtrate can effectively inhibit the growth of pathogenic bacteria staphylococcus aureus, and has the potential of regulating the skin micro-ecology.

The invention relates to polygonum multiflorum exosome-like vesicles and application thereof, the polygonum multiflorum exosome-like vesicles are applied to preparation of antioxidant products, anti-inflammatory products and anti-ultraviolet products, the antioxidant products are products for removing one or more of DPPH free radicals, ABTS free radicals and hydroxyl free radicals, the anti-inflammatory products are products for inhibiting hyaluronidase, and the anti-ultraviolet products are products for removing one or more of DPPH free radicals, ABTS free radicals and hydroxyl free radicals. And the anti-ultraviolet product is a product capable of absorbing UVB. Compared with the prior art, the polygonum multiflorum exosome-like vesicles prepared by the method disclosed by the invention are low in cost and large in treatment capacity, and the obtained polygonum multiflorum exosome-like vesicles have excellent oxidation resistance, inflammation resistance and ultraviolet resistance.

The invention relates to the field of biomedical materials, and particularly discloses a manganese dioxide nano-enzyme for treating inflammatory bowel disease as well as a preparation method and application of the manganese dioxide nano-enzyme. The preparation method of the manganese dioxide nano-enzyme comprises the following steps: uniformly mixing an organic solution containing manganese nitrate hexahydrate with a montmorillonite aqueous solution to obtain a mixed reaction solution; the pH value of the mixed reaction solution is adjusted to be under the alkaline condition through alkali liquor, a synthetic reaction is carried out, then centrifugation and washing are carried out, the montmorillonite-loaded manganese dioxide nano-enzyme is obtained, and the manganese dioxide nano-enzyme is of a lamellar structure with the length being 20-400 nm. The manganese dioxide nano enzyme has excellent activity similar to superoxide dismutase, catalase and glutathione peroxidase; meanwhile, the compound also has the capability of removing various free radicals such as DPPH and ABTS, can be used for treating the inflammatory bowel disease, and has a wide application prospect.

The invention belongs to the field of cosmetics, and discloses a composition which comprises a pepper fruit extract, epigallocatechin gallyl glucoside, diacetylboldine, a sanguisorba officinalis root extract and nicotinamide. The composition is detected by a DPPH method to have an excellent antioxidant effect. Meanwhile, the invention also discloses a composition capable of resisting thermal stimulation and UV stimulation, which can obviously inhibit the lipofuscin generation level of nematodes after thermal stimulation and UV stimulation by adopting nematode model detection. And after the composition is used together with the withanolaria nipponensis root extract, the composition has a remarkable synergistic effect on inhibition of lipofuscin generation of nematodes subjected to thermal stimulation and ultraviolet light stimulation. The composition provided by the invention has very good anti-wrinkle effects of light aging resistance and thermal aging resistance, and can effectively prevent the generation of color spots.

The invention belongs to the technical field of biological medicines, and particularly relates to an application of a Pucurbita pepo polypeptide Nacrein-LP20 in preparation of a medicine for promoting wound healing and a preparation method of the Pucurbita pepo polypeptide Nacrein-LP20. The amino acid sequence of the Hepu pearl polypeptide Nacrein-LP20 is as shown in SEQ ID NO: 1 (Sequence Identity Number 1). According to the present invention, the Pucurbita pepo active polypeptide Nacrein-LP20 has characteristics of strong DPPH free radical and hydroxyl free radical removing ability and excellent antioxidant activity, can significantly promote HaCaT cell proliferation in the high glucose environment, and can promote migration and repair of HaCaT cells in the high glucose environment; the traditional Chinese medicine composition has a remarkable healing promoting effect on full-cortical resection wound surfaces of normal rats and diabetic rats.

The invention relates to gastrodia elata fermentation liquor as well as a preparation method and application thereof, and belongs to the technical field of bioengineering. The invention provides a preparation method of gastrodia elata fermentation liquor, which comprises the following steps: (1) mixing gastrodia elata, mixed enzyme and water, and performing enzymolysis to obtain gastrodia elata enzymatic hydrolysate; and (2) inoculating zymophyte into the gastrodia elata enzymatic hydrolysate obtained in the step (1), fermenting to obtain fermentation liquor, centrifuging the fermentation liquor, taking supernate, and filtering the supernate to obtain gastrodia elata fermentation liquor. The gastrodia elata fermentation liquor obtained according to the scheme disclosed by the invention contains high concentration of p-hydroxybenzyl alcohol and parishin, so that the capacity of removing DPPH free radicals and OH-free radicals is improved, and the total antioxidant capacity of ABTS is also improved.

The present invention provides a novel antioxidant Lactiplantibacillus paracasei TDM-2, its cell-free extract and applications, belonging to the technical field of lactic acid bacteria. The Lactiplantibacillus paracasei TDM-2 provided by the present invention is deposited in the China General Microbiological Culture Collection Center, located at No. 3, Yard 1, Beichen West Road, Chaoyang District, Beijing. The deposit date is February 20, 2023, and the deposit number is CGMCC NO. 26579. The Lactiplantibacillus paracasei TDM-2 and its cell-free extract provided by the present invention have good scavenging rates of DPPH free radicals and tolerance to H2O2, and also have good reducing ability and scavenging ability for OH ‑ ions.

The invention discloses a Pediococcus pentosaceus strain and an application thereof. Belongs to the technical field of functional component conversion. According to the content of total saponin and polysaccharide in American ginseng, a lactic acid bacterium capable of efficiently converting saponin and polysaccharide is screened by taking a comprehensive score as an index, is identified as pediococcus pentosaceus through morphology, physiology, biochemistry and molecular biology and is preserved in the China General Microbiological Culture Collection Center (CGMCC), and the preservation number is CGMCC No.33430. The contents of total saponins in the transformed American ginseng and ginseng are respectively increased by 53.90% and 209.61%, and the contents of polysaccharides are respectively increased by 124.68% and 68.16%. The DPPH antioxidant capacity, the ABTS antioxidant capacity, the blood sugar reducing capacity and the blood fat reducing capacity of the converted American ginseng and ginseng total saponin extracting solution and the polysaccharide extracting solution are all improved. The pediococcus pentosaceus obtained by screening has a wide application prospect.

The invention relates to an extraction method and application of a sarcodia montagneana extract for inhibiting bacteria, removing dandruff, controlling oil and relieving, and belongs to the technical field of sarcodia montagneana extracts. The extraction method of the sarcodia montagneana extract comprises the following steps: cleaning fresh sarcodia montagneana with deionized water, soaking the cleaned sarcodia montagneana in a compound solution, grinding the soaked sarcodia montagneana to obtain slurry, freeze-drying the slurry, putting the freeze-dried slurry into a supercritical fluid extractor, adjusting corresponding temperature and pressure, and then extracting the freeze-dried sarcodia montagneana by using a supercritical fluid extractor; and fully contacting the supercritical fluid with the sarcodia montagneana powder, performing extraction treatment, and adjusting pressure through a pressure reducer for separation to obtain the sarcodia montagneana extract. The sarcodia montagneana extract contains galactose, fucose, mannose and glucuronic acid, has a remarkable removal effect on ROS and DPPH free radicals, has high antioxidant activity, can serve as a natural antioxidant and has good effects of inhibiting bacteria, removing dandruff, controlling oil and relieving.

The invention discloses a preparation method and application of a glabridin nanocomposite. The glabridin nanocomposite is based on alcohol body encapsulating glabridin, which greatly improves the stability and solubility of glabridin in a preparation. The glabridin nanocomposite is made into a gel product, which has the advantages of good flexibility, heat dissipation, skin affinity and the like, and the viscosity system of the gel reduces the aggregation of alcohol body, which can increase the stability of alcohol body, and the gel has strong antioxidant activity such as the ability to scavenge ABTS free radicals and DPPH free radicals, and the whitening effect and safety can be guaranteed, thereby promoting the application prospect of glabridin in skin whitening and freckle removal.

The invention discloses a Hepu pearl polypeptide Nacrein-LP20 for treating cataract and application of the Hepu pearl polypeptide Nacrein-LP20, and belongs to the technical field of biological medicine. The amino acid sequence of the gene is as shown in SEQ ID NO. 1. The active polypeptide Nacrein-LP20 provided by the invention has strong capability of removing DPPH free radicals and hydroxyl free radicals, and has a certain application value in the aspect of oxidation resistance. The active polypeptide Nacrein-LP20 provided by the invention has a remarkable protective effect on oxidative stress of human lens cells, can improve the cataract lesion of selenium rats, and has a certain application value in the aspect of cataract treatment.

The invention belongs to the technical field of plant fermentation, and relates to fructus arctii fermentation liquor with soothing and anti-inflammatory effects as well as a preparation method and application of the fructus arctii fermentation liquor. The fructus arctii fermentation liquid is obtained by fermenting fructus arctii through schizosaccharomyces pombe JM2-16, and the fructus arctii fermentation liquid is obtained by fermenting fructus arctii through schizosaccharomyces pombe JM2-16. According to the invention, after the fructus arctii is used as a raw material and is fermented by the schizosaccharomyces pombe, the hyaluronidase inhibition capability, the elastase inhibition capability and the DPPH free radical scavenging capability of the fermentation product can be improved, so that the fermentation product with better soothing, anti-inflammatory and anti-oxidation capabilities is obtained, and the requirements of the market on efficient, natural and safe skin care components are met.

The invention provides collagen tripeptide as well as a preparation method and application thereof, and belongs to the technical field of active peptide. The invention provides a preparation method of collagen tripeptide, which comprises the following steps: carrying out enzymolysis treatment on tilapia scales and high-temperature-resistant protease, then carrying out enzymolysis treatment by using alkaline protease and neutral protease in sequence, filtering enzymatic hydrolysate by using a ceramic membrane and an organic filter membrane in sequence, and intercepting by using a 400Da nanofiltration membrane to obtain collagen tripeptide. The permeate contains active peptide mainly composed of collagen tripeptide. After the permeate is concentrated and dried, the collagen tripeptide powder is detected, and the protein content reaches 96.2%, the GPH content is 3.8%, and the CTP content is 35.09%. The collagen tripeptide powder has better DPPH (1, 1-diphenyl-2-picrylhydrazyl) scavenging activity; the traditional Chinese medicine composition has an obvious ROS removing effect, a beta-galactosidase activity inhibiting effect and a telomerase activity enhancing promoting effect on a hydrogen peroxide induced zebrafish aging model, and shows an obvious anti-aging effect.

The invention provides colla corii asini peptide beneficial for removing free radicals of a human body and a preparation method thereof. According to the invention, three active oligopeptides are separated and purified from donkey-hide gelatin for the first time, and the donkey-hide gelatin peptide has good anti-free radical activity, can effectively remove residual DPPH free radicals and ABTS free radicals in a body, and has a great application prospect.

The invention discloses a Bacillus velez, a fermentation product filtrate thereof and a biological product thereof, belonging to the field of microbial technology. A Bacillus velez BTN-HB-F4 strain is obtained by isolating and purifying green prunes from Haba Snow Mountain in Yunnan Province. The fermentation product filtrate thereof has excellent HAS inhibition ability and significant DPPH and ABTS free radical scavenging ability, and can achieve good antioxidant effect. Secondly, at various concentrations, the fermentation product filtrate thereof can significantly reduce the proportion of SA-β-Gal-positive cells, can significantly inhibit the gene expression of matrix metalloproteinase 3, and has good anti-aging effect. In addition, the fermentation product filtrate thereof can significantly inhibit the production of zebrafish melanin, and has good whitening effect. The fermentation product filtrate can significantly inhibit the secretion of cellular inflammatory factors IL-6 and TNF-α, and has good soothing effect. Finally, the fermentation product filtrate thereof can also effectively inhibit the growth of pathogenic bacteria Staphylococcus aureus, and has the potential to regulate skin microecology.

The invention belongs to the field of biological medicines, and particularly relates to a preparation method of a pomegranate peel extract. The method comprises the following steps: pretreating fresh pomegranate rind, and extracting with mixed alcohol solvents with different concentrations. According to the extraction method provided by the invention, the pomegranate rind can be fully swelled after being soaked in the alginate oligosaccharide aqueous solution, and active ingredients in the pomegranate rind can be fully extracted after subsequent treatment, so that the extraction efficiency is improved, and the solubility and the stability of the active ingredients are improved; according to the preparation method, the mixed alcohol solvent is added in a stepped manner, so that the extraction rate of active ingredients is increased, and meanwhile, the prepared extract is increased in removal rate of DPPH free radicals and has excellent oxidation resistance.

The invention discloses a strain of Blastococcus atamensis YC-1 and application of the strain of Blastococcus atamensis YC-1, and belongs to the field of microbial strains. The streptococcus sp. YC-1 provided by the invention has high antioxidant activity and also has the capability of producing DHHB (docosahexaenoic acid); meanwhile, the YC-1FLE is prepared by adopting absolute ethyl alcohol as a solvent, has no toxicity to HaCaT cells, is relatively high in reducing capacity, has a scavenging effect on DPPH free radicals, ABTS cation free radicals and hydroxyl free radicals, has a tyrosinase inhibition effect and shows good antioxidant and whitening effects; when the YC-1FLE is applied to the sunscreen lotion, the sunscreen coefficient of the sunscreen lotion containing the YC-1FLE is obviously higher than that of a blank control group, which shows that the YC-1FLE has better ultraviolet resistance. Therefore, the strain and the lysate extract of the fermentation product of the strain have huge application potential in the field of cosmetics.

The invention discloses a corn active peptide with effects of dispelling effects of alcohol, reducing uric acid and resisting oxidation, a preparation and application of the corn active peptide, and belongs to the technical field of biological active peptides. The amino acid sequence of the corn active peptide is at least one of LMFP, FEGLFR, FLR and QLPSYR. The corn active peptide with the effects of dispelling the effects of alcohol, reducing uric acid and resisting oxidation is separated from corn germ meal through enzymolysis, ultrafiltration and other technologies, amino acid sequence determination is conducted on the separated peptide fragments, screening is conducted in combination with the bioinformatics technology, and finally four peptide fragments are obtained. According to the present invention, the corn active peptides are identified, chemical synthesis is performed according to the identified peptide fragment sequence, the effects of alcohol effect dispelling, uric acid reducing and oxidation resistance of the synthesized single peptide fragment and the compounded peptide fragment are determined, and the corn active peptides have good XOD inhibition rate, good DPPH clearance rate and good ADH activation rate, and have important application prospects in the development of the products with effects of alcohol effect dispelling, uric acid reducing and oxidation resistance.

The invention discloses a gelatinized and / or micronized nucleoside drug and a preparation method thereof, and belongs to the technical field of drugs. The preparation method comprises the following steps: mixing lipoic acid, a nucleoside drug and an esterification catalyst, carrying out a grafting reaction in a reaction solvent to obtain a lipoic acid grafted nucleoside drug, dispersing the lipoic acid grafted nucleoside drug in an organic solvent to obtain a dispersion liquid, dropwise adding the dispersion liquid into hot water, and carrying out ring opening polymerization to obtain the lipoic acid grafted nucleoside drug. The nucleoside drug nanogel, nanoparticle or nanofiber is obtained, and the structure and performance improvement based on the drug itself is realized. The gelated and / or micronized nucleoside medicine prepared by the invention has obviously improved scavenging rates of DPPH free radicals and ABTS free radicals, and the method can improve the oxidation resistance of the nucleoside medicine and has the characteristic of improving the stability and functionality of the medicine.

The invention discloses a method for increasing the extraction yield of tremella polysaccharide and application, dry tremella is used as a raw material, tremella polysaccharide is effectively extracted by utilizing an ultrasonic-cellulase wall breaking coupling green solvent method, the extraction yield of tremella polysaccharide is effectively increased, the method is green and environment-friendly and is easy to control, and the obtained tremella polysaccharide has excellent biological activity and can be used for preparing the tremella polysaccharide. The water loss rate of water can be reduced, and the moisturizing and anti-wrinkle capabilities are good; the caenorhabditis elegans can effectively remove hydroxyl free radicals, DPPH free radicals and ABTS free radicals, shows strong oxidation resistance, can significantly prolong the survival rate of caenorhabditis elegans under hydrogen peroxide stress, and shows that the caenorhabditis elegans has good oxidative stress resistance.

The invention relates to the technical field of microorganisms, in particular to lactobacillus brevicornu MPA01 capable of adsorbing microplastics and degrading uric acid and application of the lactobacillus brevicornu MPA01. The preservation number of the lactobacillus brevis MPA01 is CGMCC (China General Microbiological Culture Collection Center) No. 31977. According to the present invention, the Lactobacillus brevicornu is the marine-derived natural strain, and has significant adsorption effects on the micro-plastics PS and PE, and the Lactobacillus brevicornu MPA01 and the plant lactobacillus MPs-68 are compounded so as to provide the synergistic effect on the adsorption effects on the micro-plastics PS and PE; besides, the strain has very high DPPH free radical scavenging rate and antioxidant capacity, is resistant to gastric acid and cholate, has better intestinal colonization capacity, also has certain inhibition capacity on xanthine oxidase, has remarkable capacity of degrading uric acid, and can be applied to the field of intestinal colonization. The method has important application value in the fields of food or health care products and the like for assisting adsorption of micro-plastics and reduction of uric acid.

The invention provides application of an immature bitter orange extract in preparation of anti-oxidation and anti-inflammatory drugs. The fructus aurantii immaturus is extracted by using different solvents, and the extract is researched, so that the fructus aurantii immaturus extract has the capability of removing superoxide anion free radicals, ABTS free radicals and DPPH free radicals; in addition, the immature bitter orange extract can also reduce the content of nitric oxide, tumor necrosis factor-alpha, interleukin-1beta and interleukin-6 released by LPS-induced macrophages. The fructus aurantii immaturus extract has anti-oxidation and anti-inflammatory effects, and is used as an anti-oxidation and anti-inflammatory drug to develop a new application of an old drug of the fructus aurantii immaturus extract.

The invention provides a polypeptide beverage containing PQQ and arginine and a preparation method of the polypeptide beverage. It is found for the first time that PQQ and arginine can effectively improve the antioxidant activity of the beverage, the beverage has good DPPH free radical scavenging rate and superoxide anion scavenging rate, and a beverage product with nutritional and healthy functions can be prepared.

The invention discloses a broad-spectrum antibacterial photo-thermal biological film as well as a preparation method and application thereof, and belongs to the technical field of daily chemistry and food science. Keratin and carboxymethyl cellulose are used as raw materials, phenolated lignin nanoparticles are used as an antibacterial agent, and the prepared biological film shows excellent mechanical toughness (elongation at break gt; 150%) and self-adhesion, can form a tight package after simple pressing, has rapid photoresponse ability, can be heated to 50 DEG C or above within 1 minute, has broad-spectrum photothermal antibacterial ability and antioxidant ability, has a staphylococcus aureus and escherichia coli inhibition rate of more than 90%, and has a DPPH free radical scavenging rate gt; and the fresh-keeping period of fresh vegetables and fruits can be effectively prolonged. The prepared biological film can replace a plastic film to be applied to packaging of foods such as vegetables, fruits and grains, loss is reduced while plastic pollution is reduced, and a solution is provided for construction of a sustainable packaging system.

The invention discloses low-molecular-weight enteromorpha polysaccharide with antioxidant activity and a preparation method of the low-molecular-weight enteromorpha polysaccharide, and belongs to the technical field of polysaccharide preparation. The preparation method comprises the following steps: taking an enteromorpha crude polysaccharide solution, adding H2O2 to enable the concentration of the enteromorpha crude polysaccharide solution to be 0.04-0.08 M, and degrading for 90-120 minutes under ultraviolet rays to obtain the low-molecular-weight enteromorpha polysaccharide, the low-molecular-weight enteromorpha polysaccharide is composed of monosaccharides of rhamnose, glucose, galactose, xylose, mannose and arabinose according to the ratio of 1.08: 1.00: 0.83: 0.58: 0.69: 0.06, the scavenging capacity and the iron reducing capacity of DPPH, ABTS and hydroxyl free radicals are improved, and the low-molecular-weight enteromorpha polysaccharide is obtained. The compound has excellent antioxidant activity and can be used as or used for preparing an antioxidant active preparation. The preparation method disclosed by the invention is efficient and stable, can be used for preparing the low-molecular-weight enteromorpha polysaccharide with lighter color, lower molecular weight and higher antioxidant activity, and has higher potential application value.

The invention discloses nano-enzyme coating recombinant probiotics as well as a preparation method and application thereof, and belongs to the technical field of biotechnology and nano medicine. According to the invention, engineered Escherichia coli Nissle 1917 (ECN (DE3)) is taken as a host, and glutathione peroxidase (GPX) and glutathione reductase (GR) genes are over-expressed through a genetic engineering means, so that an efficient antioxidant enzyme system is constructed. And sequentially modifying chitosan and cerium dioxide nano-enzyme (CNP) on the surfaces of the thalli by using an electrostatic layer-by-layer self-assembly technology to form a protective coating. The nano-enzyme coating significantly improves the survival rate of the recombinant probiotics in severe environments such as simulated gastric acid (pH 2.5) and bile salt (0.3%), and enhances the ability of the recombinant probiotics to remove reactive oxygen species (ROS), including hydrogen peroxide, superoxide anions, hydroxyl free radicals and DPPH free radicals.

The invention discloses a catechol ether compound as well as a pharmaceutical composition and application thereof. The structure of the compound is as shown in a formula I, the compound further comprises pharmaceutically acceptable salt of the compound, the compound has PDE4 inhibition and antioxidation dual functions, has inhibition and removal effects on PDE4 and free radical DPPH at the same time at the micromolar concentration level, shows anti-inflammatory and antioxidative stress dual effects in vivo and in vitro, and can be used for preparing a medicine for preventing and treating PDE4 and free radical DPPH. Therefore, the compound has a wide application prospect in the aspect of treating diseases related to inflammation and oxidative stress. # imgabs0 #

The invention relates to glassine ester as well as a preparation method and application thereof. The structural formula of the glassine ester is shown as a formula (I), each R1 is independently selected from hydrogen and-C (= O) R, and at least one R1 is-C (= O) R; r is selected from C7-C21 alkyl groups and C7-C21 unsaturated chain alkyl groups. The glassine ester has a remarkable DPPH free radical scavenging effect, can remarkably improve expression of elastin, hyaluronic acid and I-type collagen in cells, and has excellent effects of oxidation resistance, moisturizing and / or tightening and wrinkle resistance when being added into cosmetics as a functional component. # imgabs0 #

The invention discloses tartary buckwheat vinasse polysaccharide with antioxidant activity and a preparation method of the tartary buckwheat vinasse polysaccharide, and belongs to the technical field of polysaccharide. The molecular weight of the tartary buckwheat vinasse polysaccharide with the antioxidant activity is 3.84 * 10 < 5 >, and the monosaccharide is prepared from Man-mannose, Rib-ribose, Rham-rhamnose, GlcUA-glucuronic acid, GalUA-galacturonic acid, Glc-glucose, Gal-galactose, Xyl-xylose and Ara-arabinose according to the molar ratio of (41.60): (20.20): (1.70): (2.84): (4.45): (8.90): (14.39): (6.27): (5.82). The tartary buckwheat vinasse polysaccharide is subjected to crude extraction through an ultrasonic-assisted water extraction and alcohol precipitation method, the extracted crude polysaccharide is purified through ion exchange column and sephadex column chromatography, and the prepared tartary buckwheat vinasse polysaccharide has a good scavenging effect on ABTS < + > free radicals, DPPH free radicals and hydroxyl free radicals and has antioxidant activity.

The invention provides application of epiphyllum callus extract in preparation of cosmetics, and belongs to the technical field of new biological materials. Compared with a common epiphyllum extract, the epiphyllum callus extract provided by the invention is rich in high-content active ingredients, such as kaempferol, general flavone, total polyphenol, total protein and total sugar; the antioxidant skincare effect is achieved, and the removal efficiency of cells on DPPH free radicals can be improved. The skin care effect of promoting ATP synthesis is achieved, ATP synthesis can be remarkably promoted, and energy metabolism of damaged mitochondria is improved. Effective components of epiphyllum calluses are extracted, the effect of the epiphyllum calluses on human immortal keratinocytes is evaluated through cell experiments, it is proved that the epiphyllum calluses have excellent effects of resisting oxidation, resisting inflammation and repairing the keratinocytes, and the epiphyllum calluses are suitable for being used as raw materials to be applied to the cosmetic industry.