635 results about "DPPH" patented technology

The invention relates to a mussel meat protein antioxidative peptide and a preparation method thereof. The invention is characterized in that the antioxidative peptide is a pentapeptide compound with an amino acid sequence of YPPAK (Tyr-Pro-Pro-Ala-Lys), and according to ESI-MS detection, a molecular ion peak of m/z [M+H]<+> 575.26 is given out. The preparation method comprises the steps consisting of slurry homogenizing, degreasing, mixing, enzymatic hydrolysis, desalination, ultrafiltration, chromatography and the like. The prepared high-activity antioxidative peptide YPPAK (Tyr-Pro-Pro-Ala-Lys) has a good removal effect on DPPH free radicals, hydroxyl free radicals and superoxide anion free radicals; meanwhile, the YPPAK (Tyr-Pro-Pro-Ala-Lys) shows a good inhibitory effect on lipid peroxidation. The YPPAK (Tyr-Pro-Pro-Ala-Lys) has the advantages of safety, no toxic and side effects, good antioxidant activity, easy digestion and absorption, etc., and can be used as a medicine, a health food or foodstuff additive and the like.

The invention discloses a process for preparing organosilicon oil which contains hydrogen, in particular to a process for preparing organosilicon oil which contains hydrogen and methyl-phenyl silica chain link. The invention utilizes dimethyl silicone cyclic siloxane(Dn), polymethyl hydrogen cyclic siloxane(DmH), hybrid ring body of methyl and phenyl (DpPh), hydrolysis hybrid ring body of methyl and phenyl (D'qPh) to be polymerization reaction monomer, the process comprises adding the polymerization reaction monomer and blocking agent into organic solvent under the protection of N2 gas, controlling the reaction temperature and the reaction time, reacting under the function of catalyst, distilling to remove dissolvent in ordinary pressure after reacting, decompressing to withdraw low-boiling residue to get target product, purifying the product with activated carbon, and then the degree of purity of the product is high. The invention has the advantages of low cost, moderate contiditon, simple technology, simple and convenient operation and no pollution, and raw material are easily obtained, and the invention is suitable for industrialization. Products which are prepared by the invention have an excellent effect in the process of sealing and packing of LED.

The invention relates to a duck wheat anti- oxidizing health- care wine, which is prepared by adding flavone extract in duck wheat- based wine. The detail process is as follows: extracting brewer's by-products with edible alcohol, condensing filterate and recovering solvent; absorbing condensed liquid with large- porous resin, washing with water and edible alcohol, collecting washing liquid, condensing and drying and getting brewer's by-products flavone extract; adding brewer's by-products flavone extract into wheat- based wine, heating for dissolution, blending and getting duck wheat anti- oxidant health- care wine. The ability to remove DPPH free residue and anti- oxidizing are increased by more than 8.1 and 33.3 times compared with original wheat- based wine, and the anti- oxidizing performance is distinctive.

The invention provides a method for preparing a traditional Chinese medicine fruit and vegetable enzyme beverage, and relates to the food field. The method comprises the steps: mixing a fruit and vegetable mixture with rock candy according to the mass ratio of 3:(0.5-1.5), placing the mixture in a fermentation tank, and in a sealed state and under a condition of the temperature of 2-30 DEG C, fermenting for 160-200 days, to obtain a fruit and vegetable fermented liquid; mixing the fruit and vegetable fermented liquid with a traditional Chinese medicine decoction liquid, inoculating with one or two or more of lactobacillus delbrueckii subsp.bulgaricus, lactobacillus plantarum subsp.plantarum, yeast and bifidobacterium animalis, and in a sealed state and at the temperature of 35-40 DEG C, fermenting for 3-8 days, to obtain the traditional Chinese medicine fruit and vegetable enzyme beverage. The medicine and food homology and mutual harmony and complementation effects are achieved; the obtained traditional Chinese medicine fruit and vegetable enzyme beverage has the advantages of high total phenol content, high DPPH(.) scavenging rate and high SOD enzyme activity, shows strong antioxidant capacity, can improve the human body gastrointestinal health-care functions, and can be used as a daily drinking health drink.

The invention discloses a method for quickly producing carotenoid by utilizing microaerobic fermentation of rhodobacter sphaeroides. The method comprises the following particular steps that the activated rhodobacter sphaeroides is inoculated to an MMS (methylmercuric sulfate) culture medium; aerobic culture is performed until OD600 (optical density 600) is 0.6-0.8; a culture condition is adjusted to be microaerobic; the carotenoid is induced to be quickly and greatly synthesized; and then extraction and purification are performed. The time for producing the carotenoid by the method is short and only 36h; the yield of thallus is up to 6g/L; the yield and the productivity of the carotenoid are 8.287mg/L and 1911mug/g respectively; the carotenoid has good capacity for removing DPPH (1,1-diphenyl-2-picryl-hydrazyl) radicals; and IC50 (half maximal inhibitory concentration) is about 8mug/mL.

The invention belongs to the technical fields of natural medicines and natural foods, in particular to a method for preparing total glycollic glucoside by using royal paulownia (original variety) fortune paulownia wood as raw material and applications of the same to foods and medicines. The total glycollic glucoside of royal paulownia (original variety) fortune paulownia wood with the purity of 40-60 percent is prepared by using the royal paulownia (original variety) fortune paulownia wood as the raw material and extracting, leaching and separating the raw material, can remarkably eliminate DPPH free radicals and can be used for preparing oxidation-resistant foods and oxidation-resistant medicines.

The invention discloses an extraction and an application of exopolysaccharide metabolite of a bacillus amyloliquefaciens C-1 strain. A 16s rRNA amplified fragment of the bacillus amyloliquefaciens C-1 strain is sequenced and compared with a 16s rRNA sequence of bacteria in a NCBI (national center of biotechnology information) database; a comparison result proves that the bacillus amyloliquefaciens C-1 strain belongs to B.amyloliquefaciens; and an NCBI register number of the 16s rRNA sequence of the bacillus amyloliquefaciens C-1 strain is JN974457. The bacillus amyloliquefaciens C-1 strain is collected in china center for type culture collection (CCTCC), has the effects of reducing exopolysaccharide, removing DPPH (1,1-diphenyl-2-picryl-hydrazyl) free radical, scavenging hydroxyl free radical (.OH), scavenging superoxide anion (O<2->), inhibiting lipid peroxidation and inhibiting human tumor cell proliferation, and opens up a new way and a new research field for exploring anti-tumor drugs.

The invention discloses a preparation method of an antioxidative peptide of a hairtail. The preparation method comprises the following steps of 1, adding water into hairtails, and mincing the hairtails into hairtail paste, 2, adding the hairtail paste into an enzymolysis tank, and adding endoprotease into the hairtail paste for hydrolysis, wherein the mass of the endoprotease is 0.2 to 0.9% of the mass of the hairtails, 3, carrying out centrifugation of a hydrolysate obtained by the step 2, and collecting a supernatant, 4, carrying out microfiltration of the supernatant, adding the micro-filtrated supernatant into the enzymolysis tank, and adding excision protease into the enzymolysis tank for hydrolysis, wherein the mass of the excision protease is 0.1 to 0.9% of the mass of the hairtails, 5, in the hydrolysis, pumping an enzymatic hydrolysate into an ultrafiltration membrane separator from the enzymolysis tank so that the enzymatic hydrolysate can be circulated at a flow of 0.1 to 0.5L/min, and 6, collecting an ultrafiltration membrane permeate, carrying out reduced pressure concentration until a volume of the ultrafiltration membrane permeate is 1/10 to 1/5 of the original volume, keeping a temperature of -18 to -20 DEG C for a night, and carrying out freeze drying at a temperature of -60 to -65 DEG C under the pressure of 0.005 to 0.0054 mbar to obtain the antioxidative peptide of hairtails. The antioxidative peptide of hairtails has strong antioxidation activity, a hydroxyl radical removal rate of 82.0%, a superoxide anion radical removal rate of 85.2% and a DPPH radical removal rate of 61.5%.

The invention relates to a method for increasing the contents of tanshinone and salvianolic acid in a salvia miltiorrhiza hairy root by using a transgene AtMYC2, belonging to the technical field of gene engineering. The method comprises the steps of constructing a high-efficiency expression vector of a plant by using an arabidopsis transcription factor AtMYC2, and carrying out genetic transformation on salvia miltiorrhiza leaves to obtain a gene AtMYC2 overexpressed transgenetic salvia miltiorrhiza hairy root; analyzing the expression of AtMYC2 in the transgenetic salvia miltiorrhiza hairy root and related genes in biosynthetic pathways of tanshinone and salvianolic acid through qRT-PCR; measuring the contents of tanshinone and salvianolic acid in the transgenetic salvia miltiorrhiza hairy root by using a high-performance liquid chromatography (HPLC); and measuring the antioxidant activity of tanshinone and salvianolic acid in the transgenetic salvia miltiorrhiza hairy root by using a DPPH free radical scavenging method. The invention provides the method for simultaneously increasing the contents of tanshinone and salvianolic acid in salvia miltiorrhiza hairy root and also provides a novel high-quality raw material for producing tanshinone and salvianolic acid with important clinic demands so as to have the positive promoting significance and application value for relieving the problem that the drug resources of tanshinone and salvianolic acid are short.

The present invention relates to emblic extract and its preparation process and application. Dried emblic powder is first microwave assisted solvent extracted, and the emblic extract is then extracted with diethyl ether and ethyl acetate and macroporous resin purified to obtain purified emblic extract. The purified emblic extract is one kind of natural antioxidant with emblic polyphenol in the content over 93 % as the main effective component. The emblic extract as natural antioxidant is superior to available synthetic antioxidants, and has high DPPH free radical eliminating activity, high lipid peroxide inhibiting activity and high peroxidation resisting capacity.

The invention discloses coumarin compounds. The preparation method comprises the following steps: extracting from rattan of Derris eriocarpa How by the inventor; according to the detection introduction of thin-layer chromatography, carrying out reversed-phase silica gel column chromatography, carrying out gradient elution by a petroleum ether-ethyl acetate system for further systematic separation, and recrystallizing. The antioxidation activity experiment proves that the compounds 1 have favorable antioxidation actions, the IC50 value for superoxide anion removal activity is less than that of the positive drug control ascorbic acid, the IC50 value DPPH.free radical removal activity has no great difference from the positive drug, and the compounds 1 have excellent antioxidation activity as compared with the coumarin compounds 6,7-dihydroxycoumarin, daphnetin and daphnoretin which are reported in documents. Therefore, the coumarin compounds disclosed by the invention can be used as a pharmaceutical or non-pharmaceutical antioxidant and the like.

The invention provides a spectrum-effect relationship-based propolis quality control method. The spectrum-effect relationship-based propolis quality control method can forecast the antioxidant activity of propolis according to a propolis fingerprint. The spectrum-effect relationship-based propolis quality control method utilizes high performance liquid chromatography (HPLC) to build a propolis fingerprint and realizes evaluation of antioxidant activity according to potassium permanganate fading time, a DPPH free radical clearance rate, a phosphomolybdic acid binding rate and a hydroxyl radical clearance rate. Through analysis of a propolis fingerprint chromatograph and spectral characteristics, the spectrum-effect relationship-based propolis quality control method determines 25 characteristic peaks and 5 characteristic peak groups. Through fitting of a polynomial, the spectrum-effect relationship-based propolis quality control method builds a mathematical model for forecasting the antioxidant activity of propolis according to a propolis fingerprint. According to the characteristic peaks and the characteristic peak groups, only through the mathematical model, the spectrum-effect relationship-based propolis quality control method can directly determine strength of the antioxidant activity of a propolis drug without an anti-oxidation test thereby determining quality. The spectrum-effect relationship-based propolis quality control method has the advantages of simple processes, strong objectivity and more characteristic peaks and realizes perfect and scientific propolis quality control.

The invention provides a preparation method of Sinonovacula constricta enzymatic hydrolysis polypeptide, and the invention is characterized in that the method: shelling Sinonovacula constricta, cleaning and rubbing, adding water according to a mass ratio which is 1:1-4 between the material and the water for homogenate, and adjusting the pH value to 7-8; adding protease for enzymatic hydrolysis, and the addition of the protease is 0.2-0.3 m% of Sinonovacula constricta, and the enzymatic hydrolysis temperature is 60-70 DEG C, and the hydrolysis time is 2-6 hours; after enzymatic hydrolysis, killing enzyme, fetching an upper clear liquid by centrifugation, and obtaining an enzymatic hydrolysis liquid; obtaining the required Sinonovacula constricta enzymatic hydrolysis polypeptide by ultrafiltration, G-25 gel chromatographic column chromatography and reversed-phase high performance liquid chromatography elution of the enzymatic hydrolysis liquid. The invention has the advantages of simple preparation technology, and the prepared Sinonovacula constricta enzymatic hydrolysis polypeptide has the advantages of high sensitivity, good stability and little side-effect, good hydroxyl radical clearance, DPPH free radical clearance and redox performance with substantial antioxidation effect; the Sinonovacula constricta enzymatic hydrolysis polypeptide can be used for external antioxidation, and has an important meaning for further research and development of functional foodstuff and medicament based on Sinonovacula constricta enzymatic hydrolysis polypeptide, and improvement of economic value of Sinonovacula constricta.

The present invention discloses a functional health edible salt added with suaeda salsal, and a preparation method thereof. The process comprises: extracting effective components of suaeda salsal with ethanol and an aqueous solution, adding the effective components to sun-baked sea salt, completely mixing, and carrying out drying crystallization to obtain the solid salt particles. According to the present invention, a ratio of the suaeda salsal solid amount to the edible salt solid weight in the suaeda salsal salt is 5-30%; a variety of minerals, trace elements, vitamins and rich amino acids can be supplemented in the daily edible salt intake process so as to provide the diet nutrition balancing effect; the obtained salt has the high polyphenol content and further has good DPPH free radical scavenging capacity, and the anti-oxidation function can provides good prevention and control effects for a lot of chronic diseases; and the advantages of natural raw materials, no addition during the production process, simple preparation process, no influence on the taste of the finished product salt, high nutrients, good absorption, stable performance, broad market prospects, and the like are provided.

The invention discloses a method for detecting free radical ration in coal tar. The method comprises the steps of removing moisture in the tar in a high speed centrifuging manner, and preserving a tar sample under low temperature or inert atmosphere; adopting a diamagnetism substance to deliquate DPPH(1,1-Diphenyl-2-picrylhydrazyl radical 2,2-Diphenyl-1-(2,4,6-trinitrophenyl) hydrazyl) to prepare a plurality of DPPH standard samples with different free radical concentrations, carrying out EPR (electron paramagnetic resonance) measurement, carrying out secondary integration on an EPR spectrogram, and recording the corresponding secondary integration area; establishing a standard curve according to the free radical contents of the DPPH standard sample with different free radical concentrations and the secondary integration area of the corresponding EPR spectrogram; and filling the tar sample into a pipe, measuring according to the same EPR measuring condition, carrying out secondary integration on the measured EPR spectrogram, and substituting the secondary integration area into the standard curve so as to obtain the free radical content N. The method provided by the invention can exactly measure free radical content in coal tar, meanwhile, the change of the content of the free radical in the coal tar along with the preservation condition is exactly measured, and the method for detecting ration is provided for the research of the free radical in the coal tar and is very simple and easy.

The invention relates to a preparation method and application of a marine fungi aspergillus terreus butyrolactone compound butyrolactone-I. The invention discloses application of the marine fungi aspergillus terreus butyrolactone compound butyrolactone-I as shown in a formula (I) to preparation of medicaments for resisting to peripheral and neurogenic inflammation and resisting to neurodegenerative diseases. The formula is shown in the description. The invention finds that besides a DPPH free radical, butyrolactone-I can also well remove an ABTS free radical and an OH free radical; the marinefungi aspergillus terreus butyrolactone compound butyrolactone-I has better oxidation resistance and inflammation resistance and has better neuroprotection activity; therefore, the marine fungi aspergillus terreus butyrolactone compound butyrolactone-I has a wide application prospect in the aspect of preparing the medicaments for resisting to peripheral and neurogenic inflammation and resisting toneurodegenerative diseases. Meanwhile, by the preparation method of the butyrolactone-I, which is provided by the invention, the butyrolactone-I can be successfully prepared; the method is simple andeasy to realize large-scale production; culture conditions can also be optimized by addition of an inducer, so that yield of the butyrolactone-I is greatly improved.

The invention relates to a preparation method of chicken bone paste zymolyte with antioxidant activity, which can obtain the zymolyte capable of effectively removed DPPH radical and hydroxy radical and has reducibility, and belongs to the technical field of biological enzymolysis. The method comprises the following steps: crushing chicken bone to prepare bone paste so as to prepare chicken bone paste suspension; heating and pre-treating the suspension for 20 to 30min at 80 to 100DEG C and cooling to the most appropriate temperature of enzyme reaction, adjusting the Ph, adding in prolease to react for a certain period of time; and increasing the temperature to 85 to 90DEG C and maintaining the temperature for 10 to 20min to inactivate enzyme to obtain light yellow powder, i.e. the chicken bone hydrolyzed protein. The chicken bone hydrolyzed protein obtained from the invention has the capability of removing DPPH free radical, hydroxyl radical and reducing, is potential natural antioxidant and functional food, has high safety, and plays the physiological roles of effectively protecting human cells and tissues and a cardiovascular circulatory system, resisting cancer, resisting aging and the like.

The invention discloses brefeldin A ester derivatives, and preparation and application thereof. The brefeldin A ester derivative compounds have antioxidation activity and antitumor activity, wherein the brefeldin A ester derivative compound disclosed as Formula (I-1), (I-3), (I-5) or (I-7) has favorable antioxidation effect and can remove DPPH, ABTS and super oxygen free radical (O2<->), and the compound (I-7) has better removal effect on DPPH, ABTS and super oxygen free radical (O2<->) than vitamin E; and the compound disclosed as Formula (I-1), (I-3), (I-5) or (I-7) has favorable inhibition activity for human lung cancer cells A549. Therefore, the result shows that the derivatives have wide application prospects in the drug development system, provide a new and broader idea for synthesizing and screening BFA (brefeldin A) derivative drugs and provide a more efficient way for treating related diseases.

The invention provides an extraction method for high-purity high-oxidative-activity aronia melanocarpa procyanidine and belongs to the technical field of extraction and purification of procyanidine. The method comprises the following steps: as aronia melanocarpa is taken as a raw material, firstly, adopting a freeze drying technology to obtain aronia melanocarpa freeze-dried powder; then, extracting the aronia melanocarpa freeze-dried powder by adopting a supercritical CO2 extraction technology; finally, purifying the aronia melanocarpa procyanidine by adopting an AB-8 type macroporous adsorption resin purification technology. According to the extraction method, the freeze drying technology and the supercritical CO2 extraction technology are adopted so that the extraction efficiency is improved and the biological activity is kept; the macroporous resin purification technology is adopted so that the extraction purity of the aronia melanocarpa procyanidine is improved and the purity reaches to 90.1%-98.6%; the cleaning capability of the aronia melanocarpa procyanidine to DPPH (1,6-bis(diphenylphosphino)hexane) is within a range from 11.2 micrograms/milliliter to 15.6 micrograms/milliliter; the obtained procyanidine is high in purity and good in activity, can be directly applied to medicines and health-care foods.

The invention relates to a hydroxypropionic acid compound and a preparation method thereof and a medical application, in particular to a (S)-2-hydroxypropionic acid-(3,4-dihydroxy phenyl) ethyl ester compound separated from medicinal blaps rynchopetera fairmaire, an extraction, separation and preparation method of the compound and an medical application of the compound to preparation of antioxidants. The compound has biological activities remarkably removing DPPH (1,1-diphenyl-2-picryhydrazyl), IC50 value of the compound removing the DPPH is 9.99+-1.61 microgrammes/milliliters and is equivalent to positive control vitamin C (IC50 value is 6.92+-0.89 microgrammes/milliliters). The compound shown as the formula (I) has novelty and great potential developing into novel antioxidants and can be expectedly developed into the antioxidants preventing and treating physiological changes or diseases related to free radicals.

The invention relates to a method for extracting effective components of chrysanthemum, in particular to a method for extracting total flavonoids of chrysanthemum. Chrysanthemum morifolium ramat as the raw material is dried and smashed, extraction is primarily conducted through enzymes and ultrasonic waves and secondarily conducted through distilled water, two extraction solutions are concentrated, ultrasonic extraction is conducted in petroleum ether, column chromatography isolation is conducted, eluant is collected, concentrating and drying are conducted, and extract of total flavonoids of chrysanthemum is obtained. The method is recyclable in solvent, easy to operate and low in cost; the obtaining rate of extract of total flavonoids of chrysanthemum is larger than or equal to 43%, the purity of total flavonoids is larger than or equal to 82%, the obtaining rate of extract of total flavonoids is high, the purity of total flavonoids is high, the removal capacity of extract of total flavonoids for DPPH is high, and the antioxidant activity of total flavonoids is high.

The present invention discloses a preparation method for increasing antioxidation activity of flammulina velutipes polysaccharide, wherein an enzymolysis control method is adopted to regulate and control molecular weight distribution of flammulina velutipes polysaccharide, flammulina velutipes is sequentially subjected to crushing, impurity removing, hot water extraction, protein removing, concentration and ethanol fractional precipitation separation to obtain the flammulina velutipes polysaccharide, and an enzyme method is adopted to carry out degradation control so as to reduce a molecular weight of the flammulina velutipes polysaccharide to 19000-30000 Da. The flammulina velutipes polysaccharide prepared by using the method is suitable for industrial production, wherein a polysaccharide extraction rate is increased by 2.5-3.5 times compared with a polysaccharide extraction rate of the traditional water extraction method, significant antioxidation activity is provided, IC50 of the flammulina velutipes polysaccharide in a DPPH.radical system is 1.69-2.12 mg/mL, and an ORAC value can be up to 923 mumol Trolox/g.

The invention discloses a carrot seed antioxidant tripeptide as well as a preparation method and an application thereof. The sequence of the carrot seed antioxidant tripeptide is Tyr-Ser-Leu (YSL). The in-vitro experiment shows that DPPH and ABTS free radicals can be effectively removed by the tripeptide, and meanwhile the tripeptide is relatively good in stability. The carrot seed antioxidant tripeptide disclosed by the invention has the characteristics of simple structure, high antioxidant activity, and the like, can be used as assistant and substitute of an artificially synthesized antioxidant, and has important values for development and application of novel antioxidant healthcare products.