103 results about "DPPH" patented technology

The invention belongs to the field of biological medicines, and particularly relates to a preparation method of a pomegranate peel extract. The method comprises the following steps: pretreating fresh pomegranate rind, and extracting with mixed alcohol solvents with different concentrations. According to the extraction method provided by the invention, the pomegranate rind can be fully swelled after being soaked in the alginate oligosaccharide aqueous solution, and active ingredients in the pomegranate rind can be fully extracted after subsequent treatment, so that the extraction efficiency is improved, and the solubility and the stability of the active ingredients are improved; according to the preparation method, the mixed alcohol solvent is added in a stepped manner, so that the extraction rate of active ingredients is increased, and meanwhile, the prepared extract is increased in removal rate of DPPH free radicals and has excellent oxidation resistance.

The invention discloses a strain of Blastococcus atamensis YC-1 and application of the strain of Blastococcus atamensis YC-1, and belongs to the field of microbial strains. The streptococcus sp. YC-1 provided by the invention has high antioxidant activity and also has the capability of producing DHHB (docosahexaenoic acid); meanwhile, the YC-1FLE is prepared by adopting absolute ethyl alcohol as a solvent, has no toxicity to HaCaT cells, is relatively high in reducing capacity, has a scavenging effect on DPPH free radicals, ABTS cation free radicals and hydroxyl free radicals, has a tyrosinase inhibition effect and shows good antioxidant and whitening effects; when the YC-1FLE is applied to the sunscreen lotion, the sunscreen coefficient of the sunscreen lotion containing the YC-1FLE is obviously higher than that of a blank control group, which shows that the YC-1FLE has better ultraviolet resistance. Therefore, the strain and the lysate extract of the fermentation product of the strain have huge application potential in the field of cosmetics.

The invention discloses nano-enzyme coating recombinant probiotics as well as a preparation method and application thereof, and belongs to the technical field of biotechnology and nano medicine. According to the invention, engineered Escherichia coli Nissle 1917 (ECN (DE3)) is taken as a host, and glutathione peroxidase (GPX) and glutathione reductase (GR) genes are over-expressed through a genetic engineering means, so that an efficient antioxidant enzyme system is constructed. And sequentially modifying chitosan and cerium dioxide nano-enzyme (CNP) on the surfaces of the thalli by using an electrostatic layer-by-layer self-assembly technology to form a protective coating. The nano-enzyme coating significantly improves the survival rate of the recombinant probiotics in severe environments such as simulated gastric acid (pH 2.5) and bile salt (0.3%), and enhances the ability of the recombinant probiotics to remove reactive oxygen species (ROS), including hydrogen peroxide, superoxide anions, hydroxyl free radicals and DPPH free radicals.

The application discloses a kind of earthworm protein with twelve 3 peptides, which is prepared by degrading earthworm protein with catalytic amount of Pro-Ala-Lys. The application also discloses the preparation method of the earthworm protein with twelve 3 peptides and the application of the earthworm protein with twelve 3 peptides in removing ·OH free radicals and DPPH free radicals. Experimental results show that the earthworm protein with twelve 3 peptides has excellent effects in removing ·OH free radicals and DPPH free radicals. Therefore, the earthworm protein with twelve 3 peptides provides an effective technical means for preparing ·OH free radical scavenger and DPPH free radical scavenger.

The invention belongs to the technical field of biology, and particularly relates to acetylated raffinose and application thereof. According to the invention, acetylation modification is carried out on raffinose by using an acetylation reagent to obtain acetylated raffinose, the acetylated raffinose can significantly improve the scavenging ability and total antioxidant ability of raffinose on DPPH free radicals, and the scavenging ability on hydroxyl free radicals and the reduction ability on iron ions are also improved to a certain extent. The acetylated raffinose provided by the invention has potential application value in development of functional foods and antioxidants.

The invention discloses an antibacterial and antioxidant sodium alginate film as well as a preparation method and application thereof, and belongs to the technical field of antibacterial materials. Epsilon-polylysine and tannic acid are synergistically compatible and are loaded on the surface of a sodium alginate matrix by adopting an atomization spraying technology, so that an antibacterial and antioxidant sodium alginate film is formed. The membrane successfully integrates the broad-spectrum antibacterial property of EPL and the strong antioxidant activity of TA, solves the problem of single function in the prior art, and realizes the synergistic interaction of antibacterial property and antioxidant property; secondly, due to the stable combination and slow release characteristics between TA and sodium alginate molecules, the membrane shows remarkable and lasting functionality, for example, the clearance rate of the obtained SA / EPL / TA membrane to DPPH free radicals can still be kept at 90% or above 7 days later, and the DPPH free radicals can be kept at 90% or above. The defect of short efficacy caused by volatilization or rapid migration of a traditional film added with a small-molecule antioxidant (such as plant essential oil) is effectively overcome.

The invention discloses protein soft sweets based on an olive pomace extract as well as a preparation method and application of the protein soft sweets. The protein soft sweets mainly consist of the following components in parts by mass: 10-50 parts of the olive pomace extract, 10-80 parts of collagen, 10-40 parts of gelatin, 1-20 parts of inulin, 1-10 parts of glucose syrup, 1-5 parts of malic acid and 1-5 parts of sodium citrate. The protein soft sweets based on the olive pomace extract have olive flavor, are amber, are crystal clear, glossy and rich in olive flavor before being sprayed with powdered sugar, and are moderate in viscosity, not sticky to teeth and good in elasticity and resilience after being sprayed with powdered sugar; the olive pomace extract prepared by the preparation method disclosed by the invention also has relatively high oxidation resistance, shows very high scavenging ability in the aspects of DPPH free radical scavenging ability and ABTS < + > free radical scavenging ability, has the free radical scavenging ability of the olive pomace extract, is beneficial to preventing oxidative damage and maintaining health, and can be used as an antioxidant functional food.

The invention discloses a method for improving the anti-oxidation characteristic of a soybean whey protein-dialdehyde starch composite film by using chlorogenic acid, and belongs to the technical field of food processing. The preparation method comprises the following steps: step 1, preparing soybean whey protein; step 2, preparation of dialdehyde starch; 3, preparing a film forming solution; and step 4, preparing the soybean whey protein-dialdehyde starch composite film added with chlorogenic acid. The chlorogenic acids with different concentrations are added into a soybean whey protein-dialdehyde starch solution to prepare the composite membrane, and the influence of the chlorogenic acids with different concentrations on the antioxidant property of the soybean whey protein-dialdehyde starch composite membrane is comprehensively evaluated by measuring the ABTS < + > and DPPH free radical scavenging rates of the composite membrane. And a theoretical basis is provided for improving the comprehensive performance of the edible film and promoting the application of novel food packaging. Comprehensive analysis shows that along with the increase of the concentration of the added chlorogenic acid, the anti-oxidation characteristic of the composite membrane is gradually enhanced, and the chlorogenic acid can effectively improve the anti-oxidation characteristic of the composite membrane.

The application discloses a strain of yeast for efficiently transforming ginsenosides and polysaccharides and application thereof, and belongs to the technical field of functional component transformation. According to the total ginsenoside and polysaccharide content in ginseng, a strain of yeast for efficiently transforming ginsenosides and polysaccharides is screened by taking a comprehensive score as an index, and is identified as Zygosaccharomyces bisporus by morphology, physiology and biochemistry and molecular biology, and is preserved in the China Center for Type Culture Collection with a preservation number of CCTCC NO: M 2020912. After transformation, the total ginsenoside content in American ginseng and ginseng is respectively increased by 67.81% and 107.03%, and the polysaccharide content is respectively increased by 238.61% and 205.87%. The DPPH antioxidant capacity, ABTS antioxidant capacity, blood sugar lowering capacity and blood lipid lowering capacity of the total ginsenoside extract and polysaccharide extract of the American ginseng and ginseng after transformation are all improved. The yeast screened by the application has a wide application prospect.

This invention aims to address the technical bottlenecks of existing milk curd products, such as weak functional activity, unclear process parameters, and unstable flavor and quality. It utilizes *Pediococcus pentosaceus* HM4, *Streptococcus thermophilus* HM6, *Lactococcus lactis* JY4, and *Lactobacillus plantarum* NC1, employing these four strains in an optimal ratio as a compound fermentation agent to achieve synergistic effects. Using milk and goji berries as raw materials, the fermentation process of milk curds and goji berry milk curds was systematically optimized through single-factor experiments, Plackett-Burman experiments, and response surface methodology. The physicochemical indicators and flavor characteristics of the products under different process conditions were analyzed. The obtained milk curds achieved a DPPH free radical scavenging rate of 91.38%–94.15%, providing technical support for the high-value utilization of milk curds and enriching the variety of functional dairy products.

This invention discloses the application of a porcine-derived Bacillus in antioxidant activity, wherein the porcine-derived Bacillus is used to prepare antioxidant products. The porcine-derived Bacillus is Bacillus subtilis YB-X-721, classified as Bacillus subtilis, and was deposited at the China General Microbiological Culture Collection Center on November 10, 2023, with accession number CGMCC No. 28942. The fermentation supernatant, bacterial suspension, and cell-free extract of the porcine-derived Bacillus subtilis YB-X-721 of this invention all possess certain DPPH free radical, superoxide anion free radical, and hydroxyl free radical scavenging abilities, as well as a certain reducing ability, and can be used to prepare antioxidant products. Furthermore, when the porcine-derived Bacillus subtilis YB-X-721 of this invention is added to piglet feed as an animal feed additive, it can improve the growth performance of weaned piglets, significantly increasing average daily weight gain, and significantly reducing average daily feed intake, feed conversion ratio, and diarrhea rate.

This invention relates to the field of peptide technology, and more particularly to a cyclic tetrapeptide compound, its preparation method, and its applications. The peptide compound has the structure shown in Formula I, or its stereoisomer or salt thereof: c(AA1-AA2-AA3-AA4), Formula I. The cyclic tetrapeptide compound can simultaneously inhibit the release of the inflammatory factor IL-6 and effectively scavenge reactive oxygen species DPPH, ABTS, and PTIO. It can effectively inhibit the production of reactive oxygen species, alleviate skin inflammation, and improve skin condition, showing great promise in the fields of anti-inflammatory and antioxidant applications.

The invention discloses an anti-oxidation and anti-aging composition and application thereof.The anti-oxidation and anti-aging composition comprises a chlorella extract and a rice chaff extract, practice shows that after the chlorella extract and the rice chaff extract are combined, the anti-oxidation and anti-aging effect can be achieved, and the obvious amplification can be achieved in an anti-oxidation test-DPPH free radical scavenging experiment; and after combination, no negative influence is caused on the activity of the fibroblast, and the expression level of the COL I of the fibroblast is obviously improved. In addition, an obvious effect can be achieved under the condition of low concentration, large-amount addition is not needed, and the effect is stable.

The invention discloses a method for predicting the turbidity tendency of beer caused by micromolecular dextrin, and relates to the technical field of beer quality detection. According to the method disclosed by the invention, micromolecular dextrin is determined as a core turbid substance through systematic analysis on beer components. According to the method, colloidal substances are separated from beer to be detected through membrane filtration and centrifugation, the essence of starch substances is verified through an enzymolysis experiment, structural characterization is conducted through Fourier transform infrared spectroscopy, nuclear magnetic resonance and other experiments, and correlation between the colloidal substances and turbidity is verified through FTIR, an electron microscope and DSC (differential scanning calorimetry). Based on the concentration of micromolecule dextrin, the concentration of tannic acid and the DPPH free radical scavenging rate, a multiple linear regression prediction equation is established, accurate prediction of the turbidity tendency of beer is achieved, and technical support is provided for beer production process optimization and quality control.

The invention discloses hump conjugated linoleic acid, a hump conjugated linoleic acid microcapsule and a preparation method and application of the hump conjugated linoleic acid microcapsule, and belongs to the technical field of deep processing of hump fat. According to the method, petroleum ether is used as a solvent, crude fat is extracted from hump fat through a Soxhlet extraction method, NaOH-ethanol is used for saponifying the crude fat, then a low-temperature crystallization method is combined with a urea adduction method, and finally, the conjugated linoleic acid is effectively separated and purified. The determination of the free radical scavenging ability of DPPH and ABTS shows that the obtained CLA has oxidation resistance. Extracted and purified conjugated linoleic acid is used as a core material, whey protein isolate and maltodextrin are selected as wall materials, an emulsion is prepared, and microcapsule powder is prepared by a direct vacuum freeze-drying method. Experiments show that the prepared conjugated linoleic acid microcapsule has a complete structure and good thermal stability.

The invention discloses an algae-derived functional active peptide, a preparation and application, and belongs to the technical field of biological active peptides. The amino acid sequence of the algae-derived functional active peptide is at least one of FPRFR and FLF. According to the preparation method, two bioactive peptides with the effects of resisting oxidation and reducing uric acid are obtained through separation and purification by taking micrococcus as a raw material, chemical synthesis is carried out according to an identified peptide fragment sequence, the effects of reducing uric acid and resisting oxidation of a synthesized single peptide fragment and a compounded peptide fragment are determined, and the bioactive peptides have relatively good XOD inhibition rate and DPPH clearance rate. Therefore, the algae-derived functional active peptides not only can be used as natural antioxidants in food, medicines and / or cosmetics, but also can be used as natural uric acid-reducing supplements in medicines; the method has an important application prospect in the development of products with the effects of reducing uric acid and resisting oxidation.

The present application belongs to the technical field of application of fungal polysaccharides, and particularly relates to a Boletus edulis fruiting body polysaccharide as well as a preparation method and application thereof. Polysaccharides in Boletus edulis fruiting bodies are extracted, and the chemical structure of BDP-F is characterized by ultraviolet-visible light, Fourier transform infrared spectroscopy, one-dimensional nuclear magnetic resonance and two-dimensional nuclear magnetic resonance. The research results show that BDP-F is mainly composed of mannose, glucose, galactose and fucose. Methylation and nuclear magnetic resonance analysis show that the glycosidic bond of BDP-F is (1→6)-alpha-d-galactose, the main chain is composed of ->6)-alpha-d-galp-(1→ residues, and the O-2 position is branched by alpha-L-Fucp-(1→ and alpha-D-Manp-(1→ residues. Through DPPH free radical, hydroxyl radical and superoxide anion radical scavenging experiments, it is shown that BDP-F has the ability to serve as a natural antioxidant, and BDP-F can also reduce alcohol-induced GES-1 cell damage through antioxidant and anti-inflammatory effects within the experimental concentration range.

This invention discloses a high-strength, tough, and antibacterial polyurethane elastomer and its preparation method. Using polyols as soft segments, isocyanates as hard segments, plant polyphenolic polyols as chain extenders, and iron salts as ligands, a high-performance polyurethane elastomer based on metal ion coordination is prepared. Due to the synergistic effect of the reversible breakage-reorganization mechanism of the dynamic coordination bonds between iron ions and phenolic hydroxyl groups and the separation of the polyurethane microphase, this polyurethane elastomer exhibits excellent mechanical properties. Furthermore, although iron ion coordination reduces the DPPH scavenging rate from 94.17% to 88.89%, it significantly enhances the photothermal conversion performance of the material. After irradiation with 808 nm near-infrared light, the PU-DVO-Fe... 1 / 4 Polyurethane achieved 100% antibacterial rate against both E. coli and S. aureus.

The invention provides a method for extracting navel orange peel polyphenol by using a deep eutectic solvent, and belongs to the technical field of natural product extraction. The method comprises the following steps: firstly, preparing a deep eutectic solvent consisting of choline chloride and ethylene glycol according to a molar ratio of 1: 2, and then extracting pretreated navel orange peel powder by using the solvent. Through optimization of a response surface method, optimal extraction process parameters are determined as follows: the water content of a solvent is 38%, the material-liquid ratio is 1: 31 g / mL, and the extraction time is 41 min. Under the conditions, the extraction yield of the navel orange peel polyphenol is high, and the extraction process is green and environment-friendly. The obtained polyphenol extract shows excellent scavenging capacity on DPPH free radicals, ABTS cation free radicals and hydroxyl free radicals, is remarkable in antioxidant activity and can be used in the fields of food, health care products, cosmetics and the like.

The invention belongs to the technical field of biochemical pharmacy, and particularly relates to a compound bezoar composition, decoction piece type compound bezoar and a preparation method of the decoction piece type compound bezoar. The compound calculus bovis composition provided by the invention comprises 94-96% by mass of main agents and 8 kinds of calculus bovis intermediates, the mass content of the auxiliary agent is 4-6%, and the auxiliary agent is six commercially available finished product combined cholic acid. The bilirubin content of the bezoar intermediate used in the invention is increased by more than 30% and the yield is increased by more than 25% compared with the existing biochemical reaction process of oxgall. The DPPH free radical scavenging rate of the compound bezoar composition reaches 89.6% + / -2.1% and is increased by 25.7-36.9% compared with 58.3% + / -3.5% of a product prepared from ox bile, and through t inspection, p is smaller than 0.01 (n is equal to 5). Free cholic acid intervention is strictly prohibited, lithocholic acid addition is completely eradicated, and the composition has the advantages of being high in bilirubin content, high in oxidation resistance and high in biological activity and adapts to the physiological characteristics of the human body.

This invention discloses a method for extracting polysaccharides from *Microcystis aeruginosa* and its applications. The method includes fat removal, alcohol-soluble molecule removal, ultrasonic water extraction, flavonoid removal, protein removal, pigment removal, ethanol precipitation, small molecule substance removal, and freeze-drying. This invention combines directional stepwise impurity removal with ultrasonic water extraction to achieve efficient separation of polysaccharides from impurities. The optimal ultrasonic extraction process was determined using response surface methodology: a fixed ultrasonic extraction power of 100W, an ultrasonic extraction temperature of 49℃, an extraction time of 61 min, a solid-liquid ratio of 1:20.5 g / mL, and three extractions. Under these conditions, the polysaccharide yield reached 1.45%. The obtained *Microcystis aeruginosa* polysaccharides exhibit significant antioxidant activity, showing good scavenging effects against DPPH free radicals, hydroxyl free radicals, and ABTS free radicals, with the total reducing power increasing in a dose-dependent manner. This invention provides a simple and controllable extraction process, yielding polysaccharides with high purity and good activity, which can be used as natural antioxidants in functional foods, health products, and cosmetics.

The invention discloses a preparation method of polypeptide with antioxidant activity, and relates to the technical field of biology. The preparation method comprises the step of preparing polypeptide with an amino acid sequence shown as SEQ ID NO.3 through a solid-phase synthesis method. According to the invention, a plurality of antioxidant active peptides are obtained by using separation and purification and mass spectrum identification technologies and by means of a saury protein database; multiple rounds of screening are carried out by utilizing the DPPH free radical scavenging rate, reducing power, water solubility, biotoxicity and other properties of the active peptide; the interaction between the antioxidant active peptide and the receptor MPO is explored by combining molecular docking, and the in-vitro antioxidant activity of the polypeptide is verified through solid-phase synthesis and cell experiments. Results show that the active peptide obtained through screening has in-vitro antioxidant activity, has good water solubility and is free of biotoxicity.

The application discloses selenium urea modified starch, a preparation method and application thereof, and belongs to the technical field of chemical industry.The structure of the selenium urea modified starch is as follows: the average value range of n is 5-12000, and R is any one of the following structures:,, and.The selenium urea modified starch has the advantages that selenium urea groups are grafted into starch through a chemical method, the obtained selenium urea modified starch has good water solubility and excellent antioxidant activity, especially has an excellent scavenging effect on DPPH free radicals, and is a good antioxidant; the preparation method has fewer steps, consumes less time and energy, and has the feasibility of large-scale preparation.

The invention discloses saccharomycetes capable of efficiently converting saponin and polysaccharide and application of the saccharomycetes. Belongs to the technical field of functional component conversion. According to the content of total saponins and polysaccharides in ginseng, a saccharomycete capable of efficiently converting saponins and polysaccharides is screened by taking a comprehensive score as an index, and the saccharomycete is identified as zygosaccharomycete dispora through morphology, physiology, biochemistry and molecular biology and is preserved in the China Center for Type Culture Collection (CCTCC) with the preservation number of CCTCC NO: M 2020912. The contents of total saponins in the transformed American ginseng and ginseng are respectively increased by 67.81% and 107.03%, and the contents of polysaccharides are respectively increased by 238.61% and 205.87%. The DPPH antioxidant capacity, the ABTS antioxidant capacity, the blood sugar reducing capacity and the blood fat reducing capacity of the converted American ginseng and ginseng total saponin extracting solution and the polysaccharide extracting solution are all improved. The saccharomycetes obtained by screening has a wide application prospect.

The invention provides Kineococcus G2 and application of the Kineococcus G2 in preparation of a pigment with antioxidant activity, the Kineococcus new strain G2 is CCTCC No. M20242602, the strain is spherical, the size is 1.0 * 1.5 mu m, the optimal growth temperature is 30-37 DEG C, the pH value is 7.0-9.0, and the salt concentration is 1.0%-2.0%. The strain G2 is not sensitive to bacitracin, ciprofloxacin and norfloxacin, and is sensitive to novobiocin, polymyxin, gentamicin, piperacillin, ofloxacin, erythromycin, streptomycin, kanamycin, rifampicin, ampicillin, vancomycin, carbenicillin, chloramphenicol, tetracycline, penicillin, cefoperazone, oxacillin, amoxicillin and neomycin. Pigment generated by fermentation of the G2 strain has good antioxidant activity, and when the concentration is 10 micrograms / mL, the scavenging activity on DPPH is 25.00%, the scavenging activity on hydroxyl radicals is 80.58%, and the iron ion reducing capacity is 1.57, which are all higher than those of contrast beta-carotenoid. The application of the new Kineococcus strain G2 can lay a foundation for the research of antioxidant drugs such as medical treatment and health care product development.

The invention discloses a Schiff base compound generated by condensation of 1-pyrenecarboxaldehyde and 4-(1, 2, 4-triazole-1-yl methyl) aniline as well as a preparation method and application of the Schiff base compound. The Schiff base compound has certain fluorescence and anti-DPPH free radical activity, and has potential application value in preparation of fluorescent materials and antioxidants.

This invention discloses a method for pretreating camellia seeds using low-temperature plasma, relating to the field of bioengineering technology. The method involves discharging the entire surface of the camellia seed using DBD, JET, DF, or RF at atmospheric pressure, preferably DBD 120kV×1min or RF 3m / min at a distance of 0-3mm for 20 passes. This achieves microporousization of the seed coat and targeted disruption of the cell wall to promote mass transfer. The treatment reduces lipase and lipoxygenase activities by approximately 31.72% and 35.69% (or 29.54%), respectively, and significantly enhances the antioxidant capacity of total phenols and DPPH / ABTS / FRAP in the methanol extract (up to 1.58 times the total phenols). This method simultaneously promotes the release of active ingredients, inhibits lipid oxidation, and improves oil yield and quality under low-temperature, non-thermal conditions, making it suitable for continuous industrial applications.

The invention belongs to the technical field of biological peptides and application, and particularly relates to an antioxidant peptide with a Keap1 inhibiting effect and application of the antioxidant peptide. The antioxidant peptide provided by the invention comprises antioxidant pentapeptide, antioxidant tripeptide and the like which all show good in-vitro antioxidant activity, the scavenging rate of ABTS free radicals reaches 0.69-1.11 [mu] mol TE / [mu] mol, the scavenging rate of DPPH free radicals reaches 30.11%-34.94%, in addition, the antioxidant peptide can activate the expression of Nrf2 and downstream genes HO-1 and NQO1 thereof by inhibiting the expression level of Keap1 protein, so that the antioxidant activity of the antioxidant peptide is improved. Therefore, the compound has a wide application prospect in the fields of cosmetics, medicines, foods and the like.

The invention relates to the technical field of health care product preparation, in particular to an anti-oxidation and anti-aging composition containing dendrobium officinale polysaccharide and application of the anti-oxidation and anti-aging composition in beauty and health care products. Comprising the following steps: mixing dendrobium officinale with water, extracting, filtering to obtain a filtrate, concentrating to obtain a concentrated solution, and carrying out alcohol precipitation to obtain a precipitate; extracting the precipitate with an extracting agent, concentrating under reduced pressure, and freeze-drying to obtain crude polysaccharide; and dissolving the crude polysaccharide with water, and sequentially loading on an ion exchange column and a gel column for purification to obtain the dendrobium officinale polysaccharide. According to the anti-oxidation and anti-aging composition containing the dendrobium officinale polysaccharide, provided by the invention, an efficient comprehensive anti-aging function is realized through a multi-target and multi-channel synergistic effect. The composition not only can strongly remove various free radicals such as DPPH free radicals, hydroxyl free radicals and superoxide anions and relieve oxidative stress injury, but also can effectively inhibit generation of melanin, has definite whitening and spot-fading effects, and is wide in application prospect.

The application discloses an antibacterial and antioxidative intelligent indication double-layer preservative film and a preparation method and application thereof. The beet red is modified to generate a beet red-acylated product, so that the beet red-acylated product has better thermal stability and stronger DPPH free radical scavenging rate. The beet red-acylated product is prepared into a first layer of preservative film together with a film-forming material. The plant essential oil is prepared into a Pickering emulsion, and then prepared into a second layer of preservative film together with the film-forming material, so that the antibacterial, antioxidative and intelligent indication double-layer preservative film is prepared.