37 results about "Aspergillus terreus" patented technology

The invention discloses a compound bacterium system and application thereof in degradation of corn straw, and relates to the technical field of microorganisms. The compound bacterium system comprises candida guilliermondii, aspergillus terreus and funalia trogii. The multifunctional efficient degradation compound bacterial system provided by the invention can efficiently degrade cellulose, hemicellulose and lignin in tobacco straws at the same time, and has no negative influence on crop growth. In the application, the high-yield strain candida guilliermondii of the biological membrane is added, so that a biological membrane compound is formed by the compound bacterial system, the straw and the straw degrading enzyme, and the synergistic degradation of lignocellulose is promoted. The compound bacterial system shows a remarkable degradation effect and application potential, the corn straw degradation rate is 38.64%, and the compound bacterial system is remarkable in contrast, and has important significance in promoting agricultural sustainable development and ecological environment protection.

The invention relates to the technical field of agricultural microorganisms, and provides a microbial agent for promoting growth of fruits and vegetables, the microbial agent is mainly prepared from a compound bacteria liquid, the total viable count of the compound bacteria liquid is greater than or equal to 1 * 10 < 10 > CFU / g, and the compound bacteria liquid comprises the following components in percentage by viable bacteria: 35-40% of aspergillus terreus, 10-20% of aspergillus niger, 5-10% of aspergillus niger, 5-10% of aspergillus niger, and the balance of water. 10 to 20% of bacillus subtilis; 15 to 20% of streptomyces sp. 10-12% of saccharomyces cerevisiae; and 18 to 25% of pseudomonas chlororaphis. The invention overcomes the defects of the prior art, is reasonable in design and relatively comprehensive in function, and also solves the technical problems that the function of the existing single microbial agent is limited and the activity of the complex microbial agent is relatively low under a high-temperature condition.

The application discloses application of a heteroterpene compound I in preparation of a heart failure resisting drug. The heteroterpene compound I is obtained by separation and purification from a marine fungus Aspergillus terreus GZU-311, and is used to treat a mouse heart failure model induced by adriamycin or left anterior descending coronary artery ligation, and it is found that the heteroterpene compound I can improve the heart function of the mouse, reduce myocardial cell damage, inhibit myocardial connective tissue thickening, improve myocardial cell arrangement disorder, reduce inflammatory cell infiltration, and has an effect of resisting heart failure. Therefore, the application provides application of the heteroterpene compound I in preparation of a heart failure resisting drug. The application enriches drug source molecules that can be used for treating heart failure, and is helpful to development of an anti-heart failure drug and clinical treatment.

The invention belongs to the technical field of medicines, and particularly relates to an application of butyrolactone metabolic ketone I separated from a rat excrement metabolite of an aspergillus terreus secondary metabolite butyrolactone I in preparation of an antioxidant drug. The compound has an obvious anti-oxidation effect; the raw materials for preparing the compound are rich in resources, the extraction and separation technical difficulty is small, the solvent can be recycled, and the production cost is low.

The invention discloses a detection method of a monacolin compound. According to the application, a non-targeted screening thought is adopted, four key daughter ions of the monacolin compound are found for the first time, and non-targeted screening methods of the monacolin compound based on key daughter ion detection are respectively established in a high-resolution mass spectrum and a triple quadrupole mass spectrum by utilizing the property that the compound is easy to crack in a source in a mass spectrum; the distribution and the content of the monacolin compound can be exclusively presented. Compared with a basic analysis method of original substances, the method has the advantages that secondary mass spectrum analysis is not needed, the retention time of the compounds can be quickly locked, and the monacolin compounds are subjected to non-targeted screening; compared with the original analysis method of the monacolin compound, the method can filter out a large part of interference of other compounds, and specifically shows the distribution and content of the monacolin compound. The method can be applied to fermentation products of monascus, aspergillus terreus and other monacolin compound producing strains and products of the fermentation products to detect distribution and relative content of the monacolin compounds, and can also be applied to quality control methods such as establishment of monacolin compound fingerprints, content determination and the like in the matrix.

Disclosed are Aspergillus terreus ANU-301 strain (Accession Number: KACC49929), and ANU-301 strain (Accession Number: KACC49929) and a composition for controlling bacterial plant diseases using the culture filtrate. Since the strain or culture filtrate, according to the present disclosure, enhances the stress resistance of plants, it enables sustainable agriculture to eliminate or minimize the use of chemical pesticides.

The present invention belongs to the field of microbial technology, and particularly relates to a phosphate-solubilizing Aspergillus terreus and its application in drought-resistant probiotics; the Aspergillus terreus of the present invention is obtained by screening the rhizosphere soil of pepper, and the Aspergillus terreus CY-05 ( Aspergillus terreus var. fluffy ) is a growth-promoting microbial strain with disease resistance, high yield of terreus ketone and phosphate solubilization ability, and is an excellent strain with strong survival ability and multiple functions such as improving crop drought tolerance. The present invention also provides a probiotic agent obtained by solid-state fermentation of Aspergillus terreus CY-05. The application of this agent can increase the tolerance of crops to drought, improve the yield and survival ability of crops under drought stress conditions, promote the conversion and utilization of insoluble phosphorus, and prevent and control the occurrence of root soil-borne diseases. Strain CY-05 is an indigenous fungus in the rhizosphere of pepper. It is non-toxic and harmless to humans and animals, does not cause environmental pollution, and helps to solve the problems of water shortage and phosphorus deficiency faced by agricultural production in large semi-arid planting areas. It has important economic significance.

This invention discloses a genetically engineered bacterium lacking the acetyltransferase gene asp12, its construction method, and its applications. This invention utilizes CRISPR / Cas9 to knock out the asp12 gene encoding acetyltransferase in Aspergillus terreus strain GZU-31-1, constructing the genetically engineered bacterium Δasp12. This invention found that compared to the wild-type Aspergillus terreus strain GZU-31-1, the genetically engineered bacterium Δasp12 exhibits increased accumulation and significantly higher yield of the compound Aspermeroterpene F, which possesses anti-inflammatory and anti-hepatic fibrosis activities. Based on the genetically engineered bacterium Δasp12, this invention also provides a method for preparing the compound Aspermeroterpene F, further improving the yield of the compound by optimizing the fermentation culture medium. This invention is beneficial for the development of anti-inflammatory and anti-hepatic fibrosis drugs.

This invention provides an itaconic acid-producing strain, its construction method, and its application. E.coli W3110, as the chassis strain, upregulated the gltA, acnA, ACS, and gatA genes, knocked out the aceA and Pta genes, downregulated the transcription level of the icd gene using the BBa_J23115 promoter, and carried the high-copy plasmid PET-28a-WAL. The plasmid was used with the T7 promoter to overexpress a gene derived from... Aspergillus terreus The strain constructed using the CAD1 gene exhibits excellent itaconic acid synthesis capabilities and stable performance. It can efficiently and stably synthesize itaconic acid de novo using glucose as a carbon source through fermentation, with low production costs and high acid production efficiency, thus achieving efficient itaconic acid production and showing excellent prospects for industrial applications.

The invention relates to the technical field of microorganisms, and particularly provides Aspergillus terreus DZ-Q1-1 and an application of the Aspergillus terreus DZ-Q1-1 in salt tolerance. The strain is an endophytic fungus separated from the root system of a halophyte, namely, sedum lineare, and is preserved in the China Center for Type Culture Collection on August 21, 2025, and the preservation number is CCTCC NO: M 20251873. Experiments show that the strain can still grow well in a culture medium containing 0.75 M of NaCl and shows strong salt tolerance. The strain and corn seedlings are co-cultured, so that the growth indexes such as plant height, root length and fresh weight of corn under salt stress can be remarkably improved, the activity of superoxide dismutase and peroxidase and the content of chlorophyll are enhanced, and cell membrane damage is reduced. The invention provides a new microbial resource for improvement of salinized soil and improvement of plant salt tolerance, and has important agricultural application value.

The present invention provides a strain of Aspergillus terreus JM-F5-6 and its application in promoting tomato growth and improving soil nutrients, belonging to the field of microbial technology. The preservation number of the Aspergillus terreus JM-F5-6 is CCTCC NO: M20232616. The conidia of the Aspergillus terreus JM-F5-6 are spherical or oval, with a smooth surface. The hyphae are colorless or slightly colored, with thick walls and septa. The hyphal branches are laterally connected to form a reticular topology. The Aspergillus terreus JM-F5-6 has strong proteolytic ability and acid-producing ability, which helps to improve the soil environment, enhance plant photosynthesis and nitrogen absorption, improve plant health and growth, and at the same time effectively increase the contents of hydrolyzable nitrogen, available phosphorus, available potassium and organic matter in the soil, improve soil fertility, and has broad market application prospects.

The invention relates to the technical field of green agriculture, in particular to a tersterione conjugated derivative as well as a preparation method and application thereof. The structural formula of the terreus ketone conjugated derivative is as shown in a formula 1, wherein R2 in the structural formula 1 comprises at least one of hydrogen, C1-3 alkyl, C2-3 alkenyl, C2-3 alkynyl, C1-3 alkoxy, C3-5 cycloalkyl, C3-5 cycloalkyloxy, halogen, nitryl, cyano, trifluoromethyl, 3-7-membered heterocyclic radical, C6-10 aryl and C6-10 heteroaryl. The structural formula of the terreus ketone conjugated derivative is as shown in a formula 1, and R2 in the structural formula 1 comprises at least one of hydrogen, C1-3 alkyl, C2-3 alkenyl, C2-3 alkynyl, C1-3 alkoxy, C3-5 cycloalkyl, C3-5 cycloalkyloxy, halogen, nitryl, cyano, trifluoromethyl, 3-7-membered heterocyclic radical, C6-10 aryl and C6-10 heteroaryl. The terleastone conjugated derivative has a good inhibition effect on radicles and germs of euphorbia lunulata, the activity of the terleastone conjugated derivative is obviously higher than that of terleastone, and the terleastone conjugated derivative has a wide application prospect in the aspect of biological weeding.

The invention belongs to the technical field of microbial strain fermentation, and particularly discloses a method for promoting aspergillus terreus growth and acid production based on exogenous amino acid. The method is used for solving the problem of long acid production period caused by low nitrogen source utilization efficiency and limited metabolic flux in the existing aspergillus terreus fermentation process. Comprising the following steps: (1) preparing a seed culture medium; (2) preparing a fermentation culture medium: adding exogenous amino acid into a seed culture medium, wherein the exogenous amino acid is one or more of L-glutamic acid, L-leucine and L-proline; (3) seed culture: inoculating the aspergillus terreus spores into a seed culture medium, and culturing for 24 hours under the conditions of 37 DEG C and 220rpm to obtain a seed solution; and (4) fermentation culture: inoculating the seed solution into a fermentation culture medium according to the inoculum size with the volume percentage concentration of 5%, and carrying out fermentation culture for 72 hours under the conditions of 37 DEG C and 220rpm. The method is easy and convenient to operate and low in cost, and the itaconic acid yield is effectively increased.

The application discloses a butenolide compound from a marine fungus, a preparation method and application thereof, and has the advantages that the butenolide compound is a novel compound discovered from metabolic products of Aspergillus terreus, similar skeleton compounds have not been reported, and the application proves for the first time that the butenolide compound has better tumor cell proliferation inhibition ability on colon cancer cells compared with other butenolide compounds.

The invention relates to the technical field of biology, in particular to yarrowia lipolytica engineering bacteria with an optimized metabolic pathway and an itaconic acid production process of the yarrowia lipolytica engineering bacteria. The engineering bacterium is constructed by taking yarrowia lipolytica as a host strain through genome multi-site modification, and the optimization of a metabolic pathway of the engineering bacterium comprises the following steps: introducing itaconic acid to synthesize a key enzyme gene: integrating a cis-aconitic acid decarboxylase gene CadA (SEQIDNO: 1) derived from Aspergillus in a strain genome, driving high-level expression through a strong promoter TEF1 and a terminator XPR2, and carrying out high-level expression through a high-level expression vector; and catalyzing the decarboxylation of intracellular cis-aconitic acid to generate itaconic acid. The metabolic bottleneck is obviously reduced, and the carbon flow continuity in the pathway is improved, so that the synthesis rate and the product cumulant of itaconic acid are effectively improved, and the problem of low acid production efficiency in a single gene expression system is solved.

This invention discloses Aspergillus terreus Aspergillus terreus Applications for reducing nicotine content. Specifically, these applications include Aspergillus terreus. Aspergillus terreus This invention relates to the method for reducing nicotine content in tobacco raw materials, tobacco waste, or tobacco processing byproducts. The method is characterized by high degradation efficiency, mild conditions, and environmental friendliness, providing a new and effective tool for biologically reducing nicotine content in tobacco leaves. It shows promising application prospects in improving tobacco quality and reducing harmful substances and tar content.

The invention discloses a recombinant yarrowia lipolytica strain with high yield of itaconic acid and a construction method and application thereof, and belongs to the field of strain cultivation, the recombinant yarrowia lipolytica strain is obtained by metabolism reconstruction of a wild type yarrowia lipolytica strain, a cis-aconitic acid decarboxylase gene cadA derived from Aspergillus terreus ATCC20542 is inserted into a genome, and a stable expression cassette is formed through a TEF1 promoter and a CYC1 terminator; a CIT1 gene front-end signal peptide is fused at the N end of the cadA to realize directional expression of mitochondria; iDH1, ACL1 and ADH1 genes are knocked out at the same time; cIT1 and ACO1 genes are overexpressed to enhance the flux of citric acid in the direction of cis-aconitic acid; and the noxE gene of Lactococcus lactis is introduced to regulate the balance of NADH (Nicotinamide Adenine Dinucleotide Hormone) in the cell. The strain is fermented for 72 hours under the conditions that glucose is used as a carbon source, the temperature is 30 DEG C and the pH value is 5.5-6.0, and the yield of itaconic acid can reach 12 g / L or above. According to the construction method, gene knockout and homologous recombination integration are realized by adopting CRISPR-Cas9, and a target strain is obtained after screening and verification. The method is used for preparing degradable polymers, plasticizers and medical intermediates. The method has the beneficial effects that the itaconic acid yield and metabolism efficiency are improved.

The present invention discloses an ω-transaminase ancestral enzyme mutant and application for efficiently catalyzing the synthesis of (R)-chiral amine compounds, relating to the field of molecular biology technology. Obtained by mutation of the ancestral enzyme Ancata-101 of the ω-transaminase from Aspergillus terreus, the amino acid sequence of the ω-transaminase mutant is shown in SEQ ID NO.6. Compared with the ancestral enzyme, the ω-transaminase ancestral enzyme mutant has significantly improved activity on non-natural substrates 1-acetylnaphthalene, N-tert-butyloxycarbonyl-3-piperidone or 3-oxopyrrolidine-1-carboxylic acid tert-butyl ester.

The invention provides a culture of aspergillus terreus in an iron-limited culture medium and application of the culture in organic pollutant treatment, and relates to the field of microorganisms and environmental remediation. According to the method, an iron-limited culture environment is adopted, aspergillus terreus is induced to secrete an iron carrier with metal chelating capacity, an iron source is captured, and therefore an efficient Fenton-like reaction system is constructed, generation of hydroxyl free radicals is promoted, and the method is suitable for in-situ green remediation of refractory organic pollutants.

The invention discloses application of aminocaproic acid to improvement of drought resistance of plants, and belongs to the field of plant stress resistance, micromolecular aminocaproic acid obtained through identification from aspergillus terreus metabolites is externally applied to roots of crops, and the drought resistance of the plants can be remarkably improved under the drought stress condition. According to the method, aminocaproic acid with effective concentration is applied in a root irrigation mode, so that crops can still keep high plant height, root length, fresh weight, stem diameter and water content in an environment with insufficient water, and inhibition of drought on plant growth is effectively relieved. Pot experiment results show that aminocaproic acid has remarkable drought-resisting and growth-promoting effects on crops such as corn and soybeans, and can improve the water steady state of plants and enhance root development. The invention further provides an agricultural composition containing aminocaproic acid and application of the agricultural composition as a drought-resistant growth promoting agent, and a new technical scheme is provided for developing novel and small-molecule stress-resistant preparations.

The present invention discloses an ω-transaminase mutant based on ancestral sequence reconstruction and relates to the technical field of molecular biology. The amino acid sequence of said ω-transaminase mutant derived from mutation of ω-transaminase from Aspergillus terreus, is as shown in SEQ ID NO. 4 or SEQ ID NO. 6. Compared with the wild-type enzyme, the half-lives of the ω-transaminase mutants are all above 24 h, while the half-life of the wild-type is only 6.90 min. The half-inactivation temperature of the mutants are 49.00° C. and 49.03° C., respectively, which are about 11° C. higher than that of the wild-type (37.89° C.), such that the thermal stability is significantly improved.

The application discloses Aspergillus terreus HBJ5-32 and application thereof in extracting total flavones from camellia sinensis, and the fermentation liquor obtained by culturing the Aspergillus terreus HBJ5-32 is filtered, and the collected filtrate is crude enzyme liquor; the crude enzyme liquor is mixed with camellia sinensis powder, and enzymolysis is carried out at 35-40 DEG C for 4-6 h; then, anhydrous ethanol is added to the enzymolysis system, and ultrasonic extraction is carried out; suction filtration is carried out, and total flavone extract liquor is obtained; the total flavone extract liquor is evaporated under reduced pressure, dissolved in anhydrous ethanol, concentrated and vacuum dried, and total flavone extract is obtained. The camellia sinensis microbial enzymolysis method provided by the application can increase the yield of total flavones extracted from camellia sinensis by 26.8% compared with the direct ethanol ultrasonic extraction method.

The amino acid sequence of a w-transaminase mutant derived from mutation of ω-transaminase from Aspergillus terreus, is as shown in SEQ ID NO. 4 or SEQ ID NO. 6. Compared with the wild-type enzyme, the half-lives of the ω-transaminase mutants are all above 24 h, while the half-life of the wild-type is only 6.90 min. The half-inactivation temperature of the mutants are 49.00° C. and 49.03° C., respectively, which are about 11° C. higher than that of the wild-type (37.89° C.), such that the thermal stability is significantly improved.

The invention relates to an application of a transporter and a gene thereof in improving tolerance of filamentous fungi to ionic liquid, and belongs to the technical field of genes. The invention aims to solve the problems that the stability of filamentous fungi in ionic liquid is poor at present, so that a fungus and ionic liquid synergistic technology is difficult to effectively apply and the like. The invention provides a transporter for improving tolerance of fungi to ionic liquid and a gene of the transporter. The nucleotide sequence of the transporter gene is shown as SEQ ID NO.1; the amino acid sequence of the transporter is as shown in SEQ ID NO. 2. After gene overexpression of the transporter provided by the invention, tolerance of filamentous fungus aspergillus terreus to ionic liquid is improved, including improvement of growth ability of a strain in the ionic liquid and improvement of activity of cellulase and hemicellulase of the strain in the ionic liquid.

The invention discloses a sacubitril intermediate synthetic transaminase mutant with high conversion rate and application thereof, and belongs to the technical field of enzyme engineering. Transaminase from aspergillus terreus is used as a starting sequence, mutation sites are screened through a novel enzyme modification technology, a series of mutants capable of improving amino transfer and sacubitril intermediate synthesis are found, heterologous expression verification is carried out after the mutants are introduced into genetic engineering escherichia coli, and the mutants can be used for improving the amino transfer and the sacubitril intermediate synthesis. Compared with a wild enzyme before mutation, when the obtained mutant enzyme is used as a catalyst to prepare a sacubitril intermediate, the substrate conversion rate is at least improved by 416.67%, the product generation rate is at least improved by 500%, and the mutant enzyme has very wide application prospects in industrial production.

This invention relates to a strain that produces exoglucanase, named *Aspergillus terreus* (…). Aspergillus terreus CB10, deposited on February 17, 2022, at the China General Microbiological Culture Collection Center (CGMCC), located at No. 3, Courtyard 1, Beichen West Road, Chaoyang District, Beijing, Institute of Microbiology, Chinese Academy of Sciences, with accession number CGMCC No. 40080. Fermentation culture using this strain can produce highly active exoglucanase, thereby expanding the range of exoglucanase-producing strains. This invention also provides a method for preparing exoglucanase by fermentation using Aspergillus terreus strain. Specifically, Aspergillus terreus strain CB10 is subjected to liquid fermentation, and the fermentation supernatant obtained after centrifugation to remove the cell bodies is the exoglucanase. The enzyme activity reaches 110.7 U / ml under conditions of pH 5.0 and 50℃. This invention is of great significance for improving the degradation efficiency of natural cellulose.

A use of a meroterpenoid compound I in the preparation of a heart failure prevention drug. By using a meroterpenoid compound I isolated and purified from the marine fungus Aspergillus terreus GZU-311 to treat mouse heart failure models induced by doxorubicin, left anterior descending coronary artery ligation, or angiotensin II, it was found that the meroterpenoid compound I can improve cardiac function in mice, reduce cardiomyocyte injury, inhibit myocardial connective tissue thickening, improve disordered arrangement of cardiomyocytes, and reduce inflammatory cell infiltration, thereby having a heart failure prevention effect. Accordingly, the use of the meroterpenoid compound I in the preparation of a heart failure prevention drug is provided. The drug source molecules that can be used for treating heart failure are enriched, which is helpful for the development of heart failure prevention drugs and clinical treatment using same.

The invention relates to the technical field of molecular detection of pathogenic microorganisms, and particularly provides a primer probe combination capable of qualitatively or quantitatively detecting four aspergillus at the same time and application of the primer probe combination. The primer probe combination comprises specific primer pairs and probes respectively aiming at aspergillus fumigatus, aspergillus flavus, aspergillus niger and aspergillus terreus. According to the invention, two efficient detection paths are established: rapid qualitative screening of'double-tube quadruple 'can be realized through a multiple fluorescent PCR method, and accurate quantitative analysis of'single-chip quadruple' can also be realized through a multiple quantitative digital PCR method. The verification result shows that aiming at four aspergillus targets, the detection limit of the multiple fluorescent PCR method can reach 75 copies / mL, and the detection limit of the multiple quantitative digital PCR method can reach 300 copies / mL. The scheme has high throughput, high sensitivity and high specificity, and an efficient integrated molecular detection solution is provided for early diagnosis and identification of invasive aspergillosis.

The invention belongs to the technical field of biological enzymes and microorganisms, and particularly relates to a chimeric enzyme and application thereof in synthesis of isocoumarin compounds. Specifically, a polyketide synthase coding gene ColB in the Chaetomium olivum SD-80A and a polyketide synthase coding gene TerA from Aspergillus terreus are used for constructing the chimeric enzyme in a structural domain replacement manner for the first time, heterologous expression of the chimeric enzyme coding gene ColB-TerA-IV is realized by constructing a heterologous expression cassette, the function of the chimeric enzyme is determined, and the polyketide synthase coding gene ColB in the Chaetomium olivum SD-80A and the polyketide synthase coding gene TerA derived from Aspergillus terreus are used for preparing the polyketide synthase. The biosynthetic pathway of the fungus metabolite isocoumarin compound normal sporine is expanded, and lead compounds can be provided for novel herbicides and related drugs, so that the method has good practical application value.

This invention provides a method for preparing trans-aconitic acid using Aspergillus terrestris fermentation and its application, relating to the field of fermentation engineering technology. By adjusting the pH and dissolved oxygen (DO) during the fermentation process, this invention can significantly increase the trans-aconitic acid content in the fermentation broth. Simultaneously, it solves the problem of excessively high production costs caused by low trans-aconitic acid content during subsequent separation and purification, greatly reducing the production cost of trans-aconitic acid, simplifying the production steps, and providing a simple and widely applicable method for trans-aconitic acid production.