207 results about "Aspergillus terreus" patented technology

The invention relates to an antibacterial mildewproof polylactic acid composition and a preparation method thereof. The composition comprises the following mixed components: polylactic acid, an antibacterial mildewproof agent, and a dispersant; wherein on a basis of 100 parts by weight of polylactic acid, the composition comprises 0.3-2 parts by weight of antibacterial mildewproof agents, and 0-0.5 parts by weight of dispersants; the antibacterial mildewproof agent is a polyguanidine pyrithione antibacterial mildewproof agent which is obtained by mixing an aqueous solution of water-soluble polyguanidine inorganic acid salts or organic acid salts and an aqueous solution of sodium pyrithione; the molar ratio of the water-soluble polyguanidine inorganic acid salts or organic acid salts and sodium pyrithione is 1:(0.1-5). The polylactic acid composition of the invention has good antibacterial and mildewproof effect. The antibacterial effect on staphylococcus aureus and escherichia coli is up to 99.9%; the mildewproof grade for mold such as aspergillus niger, aspergillus terreus, paecilomyces varioti bainier, and the like reaches 0 grade; the composition has good water resistant stability, and has wide application prospects.

The invention provides a disulfide-bond-introduced omega-aminotransferase mutant and application thereof. The amino acid sequence of the omega-aminotransferase mutant is disclosed as SEQ ID NO.1. The omega-aminotransferase mutant is obtained by respectively mutating 131st-site arginine and 134th-site aspartic acid of Aspergillus terreus omega-aminotransferase into cysteines. Compared with the wild type, the half-inactivation temperature of the omega-aminotransferase mutant is enhanced by 1.6 DEG C, the half life at 40 DEG C is 10.4 minutes which is prolonged by 3.5 minutes (50.7%), and the heat stability is greatly enhanced.

The invention discloses a high polymer quaternary phosphonium salt antibacterial material and a preparation method thereof. The high polymer quaternary phosphonium salt antibacterial material is prepared by bonding halogenacylhalide type monomers onto macromolecules of an ethylene-vinyl alcohol copolymer (EVOH) through grafting reaction and further performing quaternary phosphonium reaction with trialkylphosphine. The high polymer quaternary phosphonium salt antibacterial material disclosed by the invention has the advantages of broad-spectrum, high-efficient and lasting antibacterial function and good safety, can be shaped and processed into products with the antibacterial function alone, and mixed with polyethylene, polypropylene and other non-polar resin and other types of resin for being shaped and processed into the products with the antibacterial function, and further has broad application prospects. The sterilization efficiency of the high polymer quaternary phosphonium salt antibacterial material prepared by the preparation method disclosed by the invention against escherichia coli, staphylococcus aureus and a variety of bacteria is more than 99%, and the mildew-resistant grade against aspergillus niger, aspergillus terreus and a variety of mildew is 1-0 grade.

The invention relates to a fire-retardant polyethylene cable material with mould-proof function, which is characterized by comprising the following components by weight portion: 45 to 70 portions of high density polyethylene resin, 30 to 55 portions of linear low density polyethylene resin, 2.35 to 2.85 portions of carbon black, 35 to 65 portions of fire retardant, 3 to 6 portions of lubricant, 0.5 to 2 portions of antioxidant, and 1 to 2 portions of antibacterial agent. The method for preparing the fire-retardant polyethylene cable material with the mould-proof function comprises the following steps: weighing raw materials according to a formulation, mixing the materials, and performing the processes of extruding, granulating and drying the materials to prepare the mould-proof fire-retardant polyethylene cable material. The mould-proof fire-retardant polyethylene cable material can effectively inhibit the growth, the forming and the reproduction of eight types of mould fungi including aspergillus niger, aspergillus terreus, aureobasidium pullulans which can easily grow on a cable under the condition that the original performances and the cabling process of the fire-retardant polyethylene cable material are not influenced, thus the cable is prevented from being infringed by the mould fungi, and a number of unnecessary loss and hidden hazards to the society are avoided.

The invention discloses a biological straw decomposition agent and a preparation method thereof and particularly relates to a biological straw decomposition agent taking funguses and bacteria as raw materials and a production method of the biological straw decomposition agent. According to the biological straw decomposition agent, trichoderma longibrachiatum, aspergillus terreus, phanerochaete chrysosporium, paenibacillus amylolyticus and torulopsis candida are taken as compound stains; a cellulolytic enzyme, a semi-cellulolytic enzyme and a ligninolytic enzyme are mainly produced; the strains have good mutualistic symbiosis and metabiosis effects and have no antagonisms. When the biological straw decomposition agent is used, the decomposition agent is uniformly scattered into fields after crop straws (wheat, corns and rice) are crushed and dispersed on the ground; the dosage of the biological straw decomposition agent is 30kg per 1hm<2> and then the straws and the decomposition agent are ploughed under the ground.

The invention relates to a preparation method and application of a marine fungi aspergillus terreus butyrolactone compound butyrolactone-I. The invention discloses application of the marine fungi aspergillus terreus butyrolactone compound butyrolactone-I as shown in a formula (I) to preparation of medicaments for resisting to peripheral and neurogenic inflammation and resisting to neurodegenerative diseases. The formula is shown in the description. The invention finds that besides a DPPH free radical, butyrolactone-I can also well remove an ABTS free radical and an OH free radical; the marinefungi aspergillus terreus butyrolactone compound butyrolactone-I has better oxidation resistance and inflammation resistance and has better neuroprotection activity; therefore, the marine fungi aspergillus terreus butyrolactone compound butyrolactone-I has a wide application prospect in the aspect of preparing the medicaments for resisting to peripheral and neurogenic inflammation and resisting toneurodegenerative diseases. Meanwhile, by the preparation method of the butyrolactone-I, which is provided by the invention, the butyrolactone-I can be successfully prepared; the method is simple andeasy to realize large-scale production; culture conditions can also be optimized by addition of an inducer, so that yield of the butyrolactone-I is greatly improved.

The invention relates to improved transaminase, an encoding gene and application thereof in preparation of (R)-3-aminobutanol. The improved transaminase is higher than wild transaminase in enzymatic activity. The amino acid sequence of the wild transaminase is shown in SEQ ID No.2 and is rooted in Aspergillus terreus NIH2624. The improved transaminase comprises an amino acid sequence with at least 95% sequence homology as the amino acid sequence shown in the SEQ ID No.2, and the 215th bit corresponding to the amino acid sequence shown in SEQ ID No.2 is a proline residue. The reaction conditions are mild when the improved transaminase is used for preparing (R)-3-aminobutanol, the product is high in chirality purity and yield, the concentration of a substrate reaches up to 50 g/L, and the good industrial application prospect is achieved.

The invention discloses a novel marine fungus-derived skeleton meroterpenoid derivative and an application thereof in preparation of an anti-inflammatory drug. According to the invention, the marine fungus, namely Aspergillus terreus GZU-311 is obtained through separation and screening; and the strain is preserved in the Guangdong Microbial Culture Collection Center on December 17th, 2019, and hasa preservation number of GDMCC No: 60789. Moreover, a meroterpenoid derivative is separated and identified from a fermentation culture product of the fungus; the meroterpenoid derivative has high NOproduction resisting activity; the anti-inflammatory activity of the meroterpenoid derivative is remarkably improved by about 2 times compared with the anti-inflammatory activity of a positive controlindomethacin; meanwhile, the meroterpenoid derivative has a high safety coefficient; thus, the meroterpenoid derivative provided by the invention has good clinical application prospect in preparationof anti-inflammatory drugs, specifically in preparation of drugs for inhibiting LPS-induced NO production.

The invention relates to marine fungi aspergillus terreus C23-3, a fermentation liquor active extract as well as a preparation method and application thereof. The marine fungi aspergillus terreus C23-3 is collected in the Guangdong Microbiological Culture Collection Center (GDMCC) on Jan 22th, 2018 with the collection number GDMCC No. 60316. Active substances of standing fermentation liquor of theaspergillus terreus C23-3 are subjected to extraction and purification; a fermentation liquor total extract of a bacterial strain can well resist oxidization and restrain the activity of acetylcholinesterase; an active extract prepared by purifying the fermentation liquor of the bacterial strain has very high oxidization resistance and/or acetylcholin esterase restraining activity, and has a very good application prospect on the aspect of preparation of an anti-senile dementia medicament and on the aspect of preparation of an anti-oxidization and/or acetylcholin esterase activity restrainingmedicament.

The invention discloses a Omega-transaminase mutant and an application thereof. The amino acid sequence of the Omega-transaminase mutant is shown as SEQ ID NO.2. The Omega-transaminase mutant disclosed by the invention is acquired by respectively mutating isoleucine on locus 77, methionine on locus 150, histidine on locus 210 and methionine on locus 280 of aspergillus terreus Omega-transaminase into leucine, cysteine, asparaginate and cysteine. Semi-inactivation temperature (T5010) of the Omega-transaminase mutant is at 50.3 plus or minus 0.5 DEG C and is 11.8 DEG C higher than that of wild type; the half-life period (t1/2) at 40 DEG C is 114.6 plus or minus 2.8 min and is 16.6 times of that of wild type; heat stability is obviously promoted.

The invention provides a mutant of alpha-L-rhamnosidase from aspergillus terreus CCF 3059 and application thereof. The mutant comprises a gene D594Q shown in SEQ ID NO:2, a gene D594R shown in SEQ ID NO:3, a gene D594C shown in SEQ ID NO:4, a gene G827K shown in SEQ ID NO:5, a gene G827M shown in SEQ ID NO:6 and a gene G828A shown in SEQ ID NO:7. The mutant has the beneficial effects that the optimum temperatures of a mutant enzyme MRha-D594Q and a proenzyme MRha are 65 DEG C, but compared with the proenzyme MRha, the mutant enzyme MRha-D594Q still maintains higher enzymatic activity at 70 DEG C and 75 DEG C; the heat stability of the mutant enzyme MRha-D594Q can be further improved by adding sorbitol and is improved by 7.8 times in the half-life period at 70 DEG C.

The present invention relates to extraction and purification of lovastatin by resin through the fermentation broth from cultivation of Aspergillus terreus, which comprises the following steps: (1) Alkalizing the fermentation broth to transform lovastatin into salt, thus releasing said salt from intraspore to extraspore, followed by filtering; (2) Absorbing and extracting lovastatin from the filtrate by resin; (3) Concentrating, cyclizing, and crystallizing; (4) Centrifugating, washing, and drying the crystalline sloution; (5) Recrystallizing the crude product. In the present invention, only small amount solvent is required to provide qualified lovastatin final product, moreover the whole procedure is simple and the yield is high, thus is economic and convenient to be carried out.

The invention discloses symbiotic and epiphytic aspergillus terreus of sponge and use of aspergillus terreus for preparation of (+)- terrain. The symbiotic and epiphytic aspergillus terreus bacterial strain of the sponge is aspergillus terreus PF26CGMCC NO.5208. The step of symbiotic and epiphytic aspergillus terreus bacterial strain of the sponge for the preparation of (+)- terrain comprises a step of fermenting symbiotic and epiphytic aspergillus terreus bacterial strain of the sponge. The method concretely comprises the steps of sterilizing fermentation medium for 30 minutes at 115 DEG C; inoculating spores of aspergillus terreus PF26 into sterilized fermentation medium, wherein the inoculation amount is 4*10<6> pore/mL, and cultivating for 24 days in a shakerat 180rpm at 28 DEG C. The symbiotic and epiphytic aspergillus terreus disclosed by the invention is separated from phakellia fusca on the yongxing island on the South China Sea, and the yield of the (+)- terrain is greatly improved in comparison with the other bacterial strains at present and can be up to 3.71g/L.

The invention belongs to the technical field of bioengineering and discloses a method for increasing yield of itaconic acid produced by fermentation of aspergillus terreus. By adoption of acid-induced promoters derived from aspergillus niger, lateral respiratory chain protein AOX of the aspergillus niger is introduced into the aspergillus terreus to improve supply of an intracellular NAD+ bank, and accordingly glycolytic pathway can be enhanced to realize increase of itaconic acid yield. The itaconic acid yield of recombinant aspergillus terreus strains obtained by the method is increased by about 20% to reach 60g/L. The method provides a fresh idea for itaconic acid production.

The invention relates to a microbe and application thereof, in particular to an aspergillus terreus strain M11 for high production of cellulase, and a new method for fermenting the cellulase to produce ethanol by using the strain comprehensive bioprocessing. The technology provided by the invention comprises the following steps of: obtaining the aspergillus terreus M11 for producing heat-resisting and acid-resisting cellulose with high yield, wherein the produced cellulase has the highest enzyme activity when the pH is between 2 and 3 and the temperature is between 60 DEG C and 65 DEG C; and finishing enzyme production, hydrolysis saccharification and ethanol fermentation in one system by using the strain. According to the new comprehensive bio-processing fermentation method, the preprocessed lignocellulose is directly fermented to produce the ethanol in one step, therefore the comprehensive bio-processing fermentation is realized, and the production cost is greatly saved.

The invention provides a method for producing lovastatin by fermentation and a fermentation medium used by the same. The fermentation medium takes maltose as a carbon source and does not contain glucose. The method for producing lovastatin by fermentation by using the fermentation medium is characterized in that: Aspergillus terreus for producing lovastatin is cultured by fermentation in the fermentation medium provided by the invention. The method provided by the invention improves the fermentation unit of lovastatin by about 10%, simultaneously shortens the fermentation period, lowers the production cost, and is suitable for industrialized production.

The method to produce itaconic acid is a solid-state fermentation method. Sugarcane pressmud or peeled sugarcane pressmud is the support used to adsorb liquid medium for the production of itaconic acid by an Aspergillus terreus mutant strain. This mutant strain was derived from Aspergillus terreus ATCC 10020 by successive mutation. Beside the remaining sucrose in the sugarcane pressmud, other carbon source can be added, i.e. glucose, fructose, sucrose, or starch hydrolysate. Appropriate amounts of nitrogen source, such as ammonium nitrate, or ammonium sulfate are added. Mineral salts, such as potassium dihydrogen phosphate, magnesium sulfate, calcium sulfate, ferric chloride, zinc sulfate, and copper sulfate can be added to the medium. The suitable amount of liquid medium that can be added to the support is 4 to 6 times its dry weight for the sugarcane pressmud and 8 to 14 times its dry weight for the peeled sugarcane pressmud. The optimal pH of the medium is between 2.0-3.0. The fermentation temperature is between 30-40 degrees centigrade.

The invention relates to the field of gene engineering, and in particular relates to an itaconic acid fermentation yield improvement bacterial strain, a construction method thereof and an itaconic acid production method using the bacterial strain. The bacterial strain is recombinant aspergillus terreus obtained by inserting an aconitate decarboxylase gene in aspergillus terreus. A plasmid with a cis-aconitate decarboxylase gene expression box PgpdAt1-cad-TtrpC is constructed by gene engineering transformation, and then is inserted into the aspergillus terreus to catalyze cis-aconitate to obtain overexpression cis-aconitate decarboxylase to obtain the recombinant aspergillus terreus. The itaconic acid fermentation yield of the recombinant bacterial strain is generally higher than that of a starting bacterial strain.

The invention discloses a marine fungus, novel skeleton meroterpenoid derivatives prepared from the marine fungus as well as a preparation method and application of the novel skeleton meroterpenoid derivatives. The marine fungus Aspergillus terreus GZU-31-1 is preserved in Guangdong Microbiological Culture Collection Center on December 17, 2019 and has a preservation number of GDMCC No:60789. Sixnovel skeleton meroterpenoid derivatives are separated from fermented liquid of the marine fungus, have novel structures and are capable of remarkably reducing the activity of triglyceride; particularly, when the concentrations of the compounds 1-5 are 2 microliters and 5 microliters, the content of triglyceride in cells is remarkably reduced; especially the lipid decreasing activity of the compound 1 at a concentration of 5 microliters is even superior to that of a positive control, namely berberine at the same concentration. The compounds provided by the invention have a great application value to lipid decreasing and can be prepared into lipid decreasing drugs for use.

The invention discloses a moldproof polyethylene sheath material for cables and a preparation method thereof. The moldproof polyethylene sheath material for cables is prepared from the following raw materials in parts by weight: 80-90 parts of linear low density polyethylene, 15-20 parts of chlorosulfonated polyethylene, 10-15 parts of poly(4-methyl-1-pentene), 20-30 parts of carbon black N660, 4-8 parts of turpentine, 5-10 parts of dioctyl terephthalate, 1-2 parts of anilinomethyl triethoxy silane, 3-5 parts of methyl p-hydroxybenzoate, 8-12 parts of decabromodiphenyl oxide, 5-10 parts of zinc borate, 10-15 parts of aluminum hydroxide, 4-8 parts of brucite powder, 5-10 parts of tri(2-chloropropyl)phosphate, 2-4 parts of light magnesium oxide, 1-2 parts of tributyltin chloride, 2-3 parts of barium stearate, 3-6 parts of calcium hydroxide, 3-4 parts of oxidized polyethylene wax, 5-8 parts of quartz powder, 3-5 parts of nano silver and 4-6 parts of composite filler. On the premise that the excellent moldproof function of the sheath material is ensured, the sheath material can effectively inhibit Aspergillus niger, Aspergillus terreus and other molds from growth and reproduction, so that the cable is free from mold invasion, and the service life of the sheath is prolonged.

The present invention provides a method for preparing a moxifloxacin side chain through a biological method, particularly to a method for preparing a compound represented by a formula 2. The method comprises: catalyzing a compound represented by a formula 4 with transaminase to form a compound represented by formula 3, and carrying out spontaneous ring closure on the compound represented by the formula 3 to obtain the compound represented by the formula 2, wherein the transaminase is selected from the omega-transaminase in Arthrobacter, Aspergillus terreus, Vibrio fluvialis, Bacillus megaterium, Sphingomonas paucimobilis, Hyphomonasneptunium, and Chromobacterium violaceum, and in the formulas 2, 3 and 4, R is selected from benzyloxycarbonyl, benzyl and ethoxycarbonyl. The formulas 2, 3 and 4 are defined in the specification.

The invention discloses a gene chip for detecting 15 clinical common pathogenic microorganisms. The gene chip comprises specific detection probes fixed on a solid phase vector, wherein the probes are designed for 16SrRNA gene and ITS gene sequences of staphylococcus aureus, klebsiella pneumoniae, pseudomonas aeruginosa, proteus mirabilis, Escherichia coli, shigella, enterobacter aerogenes, pseudomonas fluorescens, candida albicans, candida glabrata, candida tropicalis, candida parapsilosis, aspergillus terreus, aspergillus flavus and rhizopus oryzae, and screened and verified by repeated tests, and have high hybridization specificity and accuracy. The chip provided by the invention ensures simple detection operations and relatively lower cost, saves time, is applied to the screening of the clinical common pathogenic microorganisms, the early rapid diagnosis of critical patients and the diagnosis of suddenly occurring infection event pathogens and favorable for clinically rapidly diagnosing infection sources and functions in the early detection and early diagnosis of clinical diseases.

The process of extracting chitosan from the waste Aspergillus terreus mycelium from itaconic acid producing process includes: pre-treating to eliminate impurity and water from the waste Aspergillus terreus mycelium, eliminating protein with dilute alkali solution at controlled temperature to obtain solid mycelium and water washing to neutrality, treating with concentrated alkali solution at controlled temperature to eliminate acetyl radical and water washing to neutrality, dissolving the neutral mycelium in dilute acid solution at controlled temperature, time and solid/liquid ratio to obtain clear solution, decolorizing with hydrogen peroxide at controlled temperature, regulating the pH value of the clear solution with alkali and separating out precipitate, dewatering the precipitate with organic solvent, and drying to obtain chitosan product. The present invention has simple technological process, high purity of the product, low power consumption and no pollution.

The invention discloses a primer and a probe of invasive mucor pathogenic bacteria, an implementation method and a detection system thereof. The method comprises the following steps of: comparing whole genome data of all strains of the obtained invasive mucor pathogenic bacteria to obtain a plurality of conserved genes of the invasive mucor pathogenic bacteria; and designing a universal primer and a probe of the invasive mucor pathogenic bacteria with specificity meeting the requirement and a species-specific primer and a probe of all strains of the invasive mucor pathogenic bacteria by utilizing the plurality of conserved genes of the invasive mucor pathogenic bacteria obtained by whole genome data comparison. By utilizing the primer and the probe designed by the invention, the sensitivity and the specificity of detection of the invasive mucor pathogenic bacteria can be improved, and various strains including aspergillus fumigatus, aspergillus flavus, aspergillus terreus, aspergillus niger and aspergillus nidulans can be detected; and the primer and the probe are suitable for different clinical samples such as BALF and FFPE tissues and exudates (pus).