24 results about "Aspergillus terreus" patented technology

The invention discloses a detection method of a monacolin compound. According to the application, a non-targeted screening thought is adopted, four key daughter ions of the monacolin compound are found for the first time, and non-targeted screening methods of the monacolin compound based on key daughter ion detection are respectively established in a high-resolution mass spectrum and a triple quadrupole mass spectrum by utilizing the property that the compound is easy to crack in a source in a mass spectrum; the distribution and the content of the monacolin compound can be exclusively presented. Compared with a basic analysis method of original substances, the method has the advantages that secondary mass spectrum analysis is not needed, the retention time of the compounds can be quickly locked, and the monacolin compounds are subjected to non-targeted screening; compared with the original analysis method of the monacolin compound, the method can filter out a large part of interference of other compounds, and specifically shows the distribution and content of the monacolin compound. The method can be applied to fermentation products of monascus, aspergillus terreus and other monacolin compound producing strains and products of the fermentation products to detect distribution and relative content of the monacolin compounds, and can also be applied to quality control methods such as establishment of monacolin compound fingerprints, content determination and the like in the matrix.

This invention discloses a genetically engineered bacterium lacking the acetyltransferase gene asp12, its construction method, and its applications. This invention utilizes CRISPR / Cas9 to knock out the asp12 gene encoding acetyltransferase in Aspergillus terreus strain GZU-31-1, constructing the genetically engineered bacterium Δasp12. This invention found that compared to the wild-type Aspergillus terreus strain GZU-31-1, the genetically engineered bacterium Δasp12 exhibits increased accumulation and significantly higher yield of the compound Aspermeroterpene F, which possesses anti-inflammatory and anti-hepatic fibrosis activities. Based on the genetically engineered bacterium Δasp12, this invention also provides a method for preparing the compound Aspermeroterpene F, further improving the yield of the compound by optimizing the fermentation culture medium. This invention is beneficial for the development of anti-inflammatory and anti-hepatic fibrosis drugs.

This invention provides an itaconic acid-producing strain, its construction method, and its application. E.coli W3110, as the chassis strain, upregulated the gltA, acnA, ACS, and gatA genes, knocked out the aceA and Pta genes, downregulated the transcription level of the icd gene using the BBa_J23115 promoter, and carried the high-copy plasmid PET-28a-WAL. The plasmid was used with the T7 promoter to overexpress a gene derived from... Aspergillus terreus The strain constructed using the CAD1 gene exhibits excellent itaconic acid synthesis capabilities and stable performance. It can efficiently and stably synthesize itaconic acid de novo using glucose as a carbon source through fermentation, with low production costs and high acid production efficiency, thus achieving efficient itaconic acid production and showing excellent prospects for industrial applications.

The invention relates to the technical field of microorganisms, and particularly provides Aspergillus terreus DZ-Q1-1 and an application of the Aspergillus terreus DZ-Q1-1 in salt tolerance. The strain is an endophytic fungus separated from the root system of a halophyte, namely, sedum lineare, and is preserved in the China Center for Type Culture Collection on August 21, 2025, and the preservation number is CCTCC NO: M 20251873. Experiments show that the strain can still grow well in a culture medium containing 0.75 M of NaCl and shows strong salt tolerance. The strain and corn seedlings are co-cultured, so that the growth indexes such as plant height, root length and fresh weight of corn under salt stress can be remarkably improved, the activity of superoxide dismutase and peroxidase and the content of chlorophyll are enhanced, and cell membrane damage is reduced. The invention provides a new microbial resource for improvement of salinized soil and improvement of plant salt tolerance, and has important agricultural application value.

The invention relates to the technical field of green agriculture, in particular to a tersterione conjugated derivative as well as a preparation method and application thereof. The structural formula of the terreus ketone conjugated derivative is as shown in a formula 1, wherein R2 in the structural formula 1 comprises at least one of hydrogen, C1-3 alkyl, C2-3 alkenyl, C2-3 alkynyl, C1-3 alkoxy, C3-5 cycloalkyl, C3-5 cycloalkyloxy, halogen, nitryl, cyano, trifluoromethyl, 3-7-membered heterocyclic radical, C6-10 aryl and C6-10 heteroaryl. The structural formula of the terreus ketone conjugated derivative is as shown in a formula 1, and R2 in the structural formula 1 comprises at least one of hydrogen, C1-3 alkyl, C2-3 alkenyl, C2-3 alkynyl, C1-3 alkoxy, C3-5 cycloalkyl, C3-5 cycloalkyloxy, halogen, nitryl, cyano, trifluoromethyl, 3-7-membered heterocyclic radical, C6-10 aryl and C6-10 heteroaryl. The terleastone conjugated derivative has a good inhibition effect on radicles and germs of euphorbia lunulata, the activity of the terleastone conjugated derivative is obviously higher than that of terleastone, and the terleastone conjugated derivative has a wide application prospect in the aspect of biological weeding.

The application discloses a butenolide compound from a marine fungus, a preparation method and application thereof, and has the advantages that the butenolide compound is a novel compound discovered from metabolic products of Aspergillus terreus, similar skeleton compounds have not been reported, and the application proves for the first time that the butenolide compound has better tumor cell proliferation inhibition ability on colon cancer cells compared with other butenolide compounds.

The invention relates to the technical field of biology, in particular to yarrowia lipolytica engineering bacteria with an optimized metabolic pathway and an itaconic acid production process of the yarrowia lipolytica engineering bacteria. The engineering bacterium is constructed by taking yarrowia lipolytica as a host strain through genome multi-site modification, and the optimization of a metabolic pathway of the engineering bacterium comprises the following steps: introducing itaconic acid to synthesize a key enzyme gene: integrating a cis-aconitic acid decarboxylase gene CadA (SEQIDNO: 1) derived from Aspergillus in a strain genome, driving high-level expression through a strong promoter TEF1 and a terminator XPR2, and carrying out high-level expression through a high-level expression vector; and catalyzing the decarboxylation of intracellular cis-aconitic acid to generate itaconic acid. The metabolic bottleneck is obviously reduced, and the carbon flow continuity in the pathway is improved, so that the synthesis rate and the product cumulant of itaconic acid are effectively improved, and the problem of low acid production efficiency in a single gene expression system is solved.

This invention discloses Aspergillus terreus Aspergillus terreus Applications for reducing nicotine content. Specifically, these applications include Aspergillus terreus. Aspergillus terreus This invention relates to the method for reducing nicotine content in tobacco raw materials, tobacco waste, or tobacco processing byproducts. The method is characterized by high degradation efficiency, mild conditions, and environmental friendliness, providing a new and effective tool for biologically reducing nicotine content in tobacco leaves. It shows promising application prospects in improving tobacco quality and reducing harmful substances and tar content.

The invention discloses a recombinant yarrowia lipolytica strain with high yield of itaconic acid and a construction method and application thereof, and belongs to the field of strain cultivation, the recombinant yarrowia lipolytica strain is obtained by metabolism reconstruction of a wild type yarrowia lipolytica strain, a cis-aconitic acid decarboxylase gene cadA derived from Aspergillus terreus ATCC20542 is inserted into a genome, and a stable expression cassette is formed through a TEF1 promoter and a CYC1 terminator; a CIT1 gene front-end signal peptide is fused at the N end of the cadA to realize directional expression of mitochondria; iDH1, ACL1 and ADH1 genes are knocked out at the same time; cIT1 and ACO1 genes are overexpressed to enhance the flux of citric acid in the direction of cis-aconitic acid; and the noxE gene of Lactococcus lactis is introduced to regulate the balance of NADH (Nicotinamide Adenine Dinucleotide Hormone) in the cell. The strain is fermented for 72 hours under the conditions that glucose is used as a carbon source, the temperature is 30 DEG C and the pH value is 5.5-6.0, and the yield of itaconic acid can reach 12 g / L or above. According to the construction method, gene knockout and homologous recombination integration are realized by adopting CRISPR-Cas9, and a target strain is obtained after screening and verification. The method is used for preparing degradable polymers, plasticizers and medical intermediates. The method has the beneficial effects that the itaconic acid yield and metabolism efficiency are improved.

The invention provides a culture of aspergillus terreus in an iron-limited culture medium and application of the culture in organic pollutant treatment, and relates to the field of microorganisms and environmental remediation. According to the method, an iron-limited culture environment is adopted, aspergillus terreus is induced to secrete an iron carrier with metal chelating capacity, an iron source is captured, and therefore an efficient Fenton-like reaction system is constructed, generation of hydroxyl free radicals is promoted, and the method is suitable for in-situ green remediation of refractory organic pollutants.

The invention discloses application of aminocaproic acid to improvement of drought resistance of plants, and belongs to the field of plant stress resistance, micromolecular aminocaproic acid obtained through identification from aspergillus terreus metabolites is externally applied to roots of crops, and the drought resistance of the plants can be remarkably improved under the drought stress condition. According to the method, aminocaproic acid with effective concentration is applied in a root irrigation mode, so that crops can still keep high plant height, root length, fresh weight, stem diameter and water content in an environment with insufficient water, and inhibition of drought on plant growth is effectively relieved. Pot experiment results show that aminocaproic acid has remarkable drought-resisting and growth-promoting effects on crops such as corn and soybeans, and can improve the water steady state of plants and enhance root development. The invention further provides an agricultural composition containing aminocaproic acid and application of the agricultural composition as a drought-resistant growth promoting agent, and a new technical scheme is provided for developing novel and small-molecule stress-resistant preparations.

The application discloses Aspergillus terreus HBJ5-32 and application thereof in extracting total flavones from camellia sinensis, and the fermentation liquor obtained by culturing the Aspergillus terreus HBJ5-32 is filtered, and the collected filtrate is crude enzyme liquor; the crude enzyme liquor is mixed with camellia sinensis powder, and enzymolysis is carried out at 35-40 DEG C for 4-6 h; then, anhydrous ethanol is added to the enzymolysis system, and ultrasonic extraction is carried out; suction filtration is carried out, and total flavone extract liquor is obtained; the total flavone extract liquor is evaporated under reduced pressure, dissolved in anhydrous ethanol, concentrated and vacuum dried, and total flavone extract is obtained. The camellia sinensis microbial enzymolysis method provided by the application can increase the yield of total flavones extracted from camellia sinensis by 26.8% compared with the direct ethanol ultrasonic extraction method.

The amino acid sequence of a w-transaminase mutant derived from mutation of ω-transaminase from Aspergillus terreus, is as shown in SEQ ID NO. 4 or SEQ ID NO. 6. Compared with the wild-type enzyme, the half-lives of the ω-transaminase mutants are all above 24 h, while the half-life of the wild-type is only 6.90 min. The half-inactivation temperature of the mutants are 49.00° C. and 49.03° C., respectively, which are about 11° C. higher than that of the wild-type (37.89° C.), such that the thermal stability is significantly improved.

The invention relates to an application of a transporter and a gene thereof in improving tolerance of filamentous fungi to ionic liquid, and belongs to the technical field of genes. The invention aims to solve the problems that the stability of filamentous fungi in ionic liquid is poor at present, so that a fungus and ionic liquid synergistic technology is difficult to effectively apply and the like. The invention provides a transporter for improving tolerance of fungi to ionic liquid and a gene of the transporter. The nucleotide sequence of the transporter gene is shown as SEQ ID NO.1; the amino acid sequence of the transporter is as shown in SEQ ID NO. 2. After gene overexpression of the transporter provided by the invention, tolerance of filamentous fungus aspergillus terreus to ionic liquid is improved, including improvement of growth ability of a strain in the ionic liquid and improvement of activity of cellulase and hemicellulase of the strain in the ionic liquid.

The invention discloses a sacubitril intermediate synthetic transaminase mutant with high conversion rate and application thereof, and belongs to the technical field of enzyme engineering. Transaminase from aspergillus terreus is used as a starting sequence, mutation sites are screened through a novel enzyme modification technology, a series of mutants capable of improving amino transfer and sacubitril intermediate synthesis are found, heterologous expression verification is carried out after the mutants are introduced into genetic engineering escherichia coli, and the mutants can be used for improving the amino transfer and the sacubitril intermediate synthesis. Compared with a wild enzyme before mutation, when the obtained mutant enzyme is used as a catalyst to prepare a sacubitril intermediate, the substrate conversion rate is at least improved by 416.67%, the product generation rate is at least improved by 500%, and the mutant enzyme has very wide application prospects in industrial production.

This invention relates to a strain that produces exoglucanase, named *Aspergillus terreus* (…). Aspergillus terreus CB10, deposited on February 17, 2022, at the China General Microbiological Culture Collection Center (CGMCC), located at No. 3, Courtyard 1, Beichen West Road, Chaoyang District, Beijing, Institute of Microbiology, Chinese Academy of Sciences, with accession number CGMCC No. 40080. Fermentation culture using this strain can produce highly active exoglucanase, thereby expanding the range of exoglucanase-producing strains. This invention also provides a method for preparing exoglucanase by fermentation using Aspergillus terreus strain. Specifically, Aspergillus terreus strain CB10 is subjected to liquid fermentation, and the fermentation supernatant obtained after centrifugation to remove the cell bodies is the exoglucanase. The enzyme activity reaches 110.7 U / ml under conditions of pH 5.0 and 50℃. This invention is of great significance for improving the degradation efficiency of natural cellulose.

A use of a meroterpenoid compound I in the preparation of a heart failure prevention drug. By using a meroterpenoid compound I isolated and purified from the marine fungus Aspergillus terreus GZU-311 to treat mouse heart failure models induced by doxorubicin, left anterior descending coronary artery ligation, or angiotensin II, it was found that the meroterpenoid compound I can improve cardiac function in mice, reduce cardiomyocyte injury, inhibit myocardial connective tissue thickening, improve disordered arrangement of cardiomyocytes, and reduce inflammatory cell infiltration, thereby having a heart failure prevention effect. Accordingly, the use of the meroterpenoid compound I in the preparation of a heart failure prevention drug is provided. The drug source molecules that can be used for treating heart failure are enriched, which is helpful for the development of heart failure prevention drugs and clinical treatment using same.

The invention relates to the technical field of molecular detection of pathogenic microorganisms, and particularly provides a primer probe combination capable of qualitatively or quantitatively detecting four aspergillus at the same time and application of the primer probe combination. The primer probe combination comprises specific primer pairs and probes respectively aiming at aspergillus fumigatus, aspergillus flavus, aspergillus niger and aspergillus terreus. According to the invention, two efficient detection paths are established: rapid qualitative screening of'double-tube quadruple 'can be realized through a multiple fluorescent PCR method, and accurate quantitative analysis of'single-chip quadruple' can also be realized through a multiple quantitative digital PCR method. The verification result shows that aiming at four aspergillus targets, the detection limit of the multiple fluorescent PCR method can reach 75 copies / mL, and the detection limit of the multiple quantitative digital PCR method can reach 300 copies / mL. The scheme has high throughput, high sensitivity and high specificity, and an efficient integrated molecular detection solution is provided for early diagnosis and identification of invasive aspergillosis.

The invention belongs to the technical field of biological enzymes and microorganisms, and particularly relates to a chimeric enzyme and application thereof in synthesis of isocoumarin compounds. Specifically, a polyketide synthase coding gene ColB in the Chaetomium olivum SD-80A and a polyketide synthase coding gene TerA from Aspergillus terreus are used for constructing the chimeric enzyme in a structural domain replacement manner for the first time, heterologous expression of the chimeric enzyme coding gene ColB-TerA-IV is realized by constructing a heterologous expression cassette, the function of the chimeric enzyme is determined, and the polyketide synthase coding gene ColB in the Chaetomium olivum SD-80A and the polyketide synthase coding gene TerA derived from Aspergillus terreus are used for preparing the polyketide synthase. The biosynthetic pathway of the fungus metabolite isocoumarin compound normal sporine is expanded, and lead compounds can be provided for novel herbicides and related drugs, so that the method has good practical application value.

The invention discloses a synthetic bacterial agent for preventing and controlling tobacco black shank and relieving successive cropping obstacles as well as a preparation method and application thereof, and belongs to the technical field of biological prevention and control of plant diseases. The synthetic bacterial agent is prepared by mixing Aspergillus terreus JD-6, Aspergillus terreus JD-8, Aspergillus fumigatus JD-33, Actinomucor elegans JD-25, Mucor circinelloides JD-27 and Penicillium chrysogenum JD-38 according to the proportion of biomass and the like, and the synthetic bacterial agent is prepared by mixing Aspergillus terreus JD-6, Aspergillus terreus JD-8, Aspergillus fumigatus JD-33, Actinomucor elegans JD-25, Mucor circinelloides JD-27 and Penicillium chrysogenum JD-38 according to the proportion of biomass and the like. The synthetic microbial agent provided by the invention has no antagonistic effect among microbial members, can significantly inhibit the growth of phytophthora nicotianae, reduces the incidence rate of tobacco and promotes the growth of continuous cropping tobacco, and has an effect superior to that of a single microbial strain.

The invention relates to the technical field of green agriculture, in particular to an aspergillus terreus ketone ester derivative as well as a preparation method and application thereof. The structural formula of the terreus ketone ester derivative is as shown in a formula 1, wherein the structure of R1 in the structural formula 1 comprises at least one of hydrogen, C1-3 alkyl, C2-3 alkenyl, C2-3 alkynyl, C1-3 alkoxy, C3-5 cycloalkyl, C3-5 cycloalkyloxy, halogen, nitryl, cyano, trifluoromethyl, 3-7-membered heterocyclic radical, C6-10 aryl and C6-10 heteroaryl. The prevention and control effect of the aspergillus terreus ketone ester derivative is remarkably improved, the solubility is increased, the weed prevention and control spectrum is expanded, and the aspergillus terreus ketone ester derivative has the potential of being developed into a microbial herbicide.

This invention belongs to the field of bioenzyme and microbial technology, specifically relating to a chimeric enzyme and its application in the synthesis of isocoumarin compounds. Specifically, this invention is the first to utilize olive-colored hairy shells (… Chaetomium olivaceum A polyketide synthase encoding gene in SD-80A ColB With Aspergillus terreus ( Aspergillus terreus A polyketide synthase encoding gene from ( ) TerA Chimeric enzymes were constructed using domain substitution, and heterologous expression cassettes were built to achieve expression of the chimeric enzyme encoding gene. ColB‑TerA‑Ⅳ The heterologous expression of this chimeric enzyme confirmed its function, expanded the biosynthetic pathway of orthosporain, a fungal metabolite, and helped provide lead compounds for novel herbicides and related drugs, thus having good practical application value.

This invention discloses a bamboo vinegar powder extract and its identification method and application. The bamboo vinegar powder extract contains at least one of pyrogallol, 4-methylcatechol, p-hydroxyphenylethanol, p-methylbenzoic acid, dihydrocaffeic acid, and 1,4-dibenzene. Among them, 4-methylcatechol and p-methylbenzoic acid have broad-spectrum antifungal effects, exhibiting very good antifungal activity against Aspergillus terreus CICC40377, Trichoderma longibrachiatum CICC40380, and Paecilomyces variotii CICC40716.

The invention relates to a peculiar smell removing agent based on complex zinc and a preparation method of the peculiar smell removing agent, and relates to the technical field of environment purification. 5-35 parts of a main complexing agent; 0-20 parts of an auxiliary complexing agent; 5 to 15 parts of an auxiliary agent; 1-5 parts of a pH regulator; and the balance of deionized water. Wherein the zinc salt, the main complexing agent and the auxiliary complexing agent form complex zinc in the solution; the main complexing agent is prepared by fermenting aspergillus terreus and then is a polyhydroxy-carboxyl compound which is subjected to ultrafiltration purification by an ultrafiltration membrane and has the content of more than or equal to 99%; the auxiliary complexing agent is selected from at least one of L-glutamic acid and sodium gluconate. According to the invention, through a complexing system zinc complex, the problems that a traditional deodorant is easy to hydrolyze and precipitate and poor in water solubility are solved, the deodorant shows excellent removal efficiency on malodorous gases such as ammonia gas, hydrogen sulfide and sulfur-containing organic matters, and meanwhile, the deodorant has good storage stability and use safety.