562 results about "Pseudomona aeruginosa" patented technology

The current invention provides high-affinity antibodies to the Pseudomonas aeruginosa PcrV protein that have reduced immunogenicity when administered to treat Pseudomonas aeruginosa infections.

The invention discloses a pseudomonas aeruginosa D10 which is preserved in China Typical Culture Preservation Center, and the preservation number is CCTCC NO: M 209104. A colony on a beef paste peptone culture medium appears brown, round, glossy and wet, and has trim edges; the diameter of the colony is 0.2-0.5 cm; and the thallus is in a short bar shape and does not have gemma. The invention also discloses a preparation method and applications of the pseudomonas aeruginosa D10. The pseudomonas aeruginosa D10 of the invention can inhibit corn northern and southern leaf blight, has strong antergic functions on corn northern leaf blight, corn southern leaf blight, corn curvalaria leaf spot, cotton verticillium wilt, wheat fusarium graminearum, banana colletotrichum toilette and banana fusarium wilt, has the advantages of wide bacteriostatic range, high prevention efficiency and favorable environmental safety, and has favorable prospects for development and applications.

The invention discloses a Pseudomonas aeruginosa strain TBPY, which is screened from tribromophenol(TBP)-polluted sludge and purified. The purified strain is domesticated by a pressure type domesticating method with gradually increased TBP concentration in an organic salt culture medium, and the domesticated strain can be used for the biological control of waste water containing persistent organic pollutants such as phenol, pyrocatechol, resorcinol, benzoic acid, p-hydroxybenzoic acid, vanillin, 4-chlorophenol, 2,4-dichlorophenol, trichlorophenol and tribromophenol and has high research and application values.

The invention discloses a strong emulsibility microbe wax cleaning and preventing bacterial agent and an application, which belongs to the oil field chemical technology field. The wax cleaning and preventing bacterial agent mainly comprises pseudomonas aeruginosa and geobacillus sp, has strong emulsification capability, and is capable of dispersing crude oil, promoting crude oil to flow, changing adherence force of borehole wall, preventing the accumulation of wax crystal on the borehole wall, and playing the paraffin inhibition and wax cleaning effects. By increasing the initial application amount of the bacteria liquid and prolong the well closing time, a stable paraffin inhibition zone can be formed due to adhesion of bacteria on borehole wall, near wellbore formation can be cleaned, and the paraffin inhibition and oil increase effects can be increased. After on-site enforcement, the well cleaning and chemical paraffin inhibition works can not be carried out in recent half year in the test wells, so that the wax cleaning and preventing bacterial agent has good paraffin inhibition effect in the well. The oil production is increased by about 30% daily, pump efficiency is increased about 10%, the surface tension of the output liquid is decreased to 20-25%, and the crude oil condensation point is decreased by 1-3 DEG C.

The invention discloses pseudomonas aeruginosa and an application thereof in degrading zearalenone. The preservation number of the pseudomonas aeruginosa is CGMCC No.8727. As a biologic material for degrading zearalenone, the pseudomonas aeruginosa is good in application prospect in no matter developing a novel biologic degrading bacterium agent or a biologic degrading bacterium-free preparation.

The present invention provides a gene chip for quickly detecting pathogens from food source, discloses the preparation method of said gene chip and provides 26 oligonucleotide probe sequences for detection. Said gene chip can quickly, accurately and high-effectively detect and identify the class of the pathogens in food, and its detection range includes staphylococcus aureus, Shiga's bacillus, salmonella, colibacillus 0157, bacillus proteus, mononuclear hyperplastic listerella, enterocolitis yersinia, aeruginous pseudomonads, vibrio parahaemolyticus, vibrio cholerae, bacillus cereus, beta hemolytic streptococcus, coconut fermentation pseudomonads, boticin, vibrio jejuni and bacillus perfringens, etc.

Provided is a novel compound which is useful as a pharmaceutical composition by inhibiting an LpxC activity, thereby exhibiting potent antimicrobial activity against gram-negative bacteria including Pseudomonas aeruginosa and its drug resistant bacteria. Provided is a hydroxamic acid derivative represented by the following general formula [1] or a pharmaceutically acceptable salt thereof:

The invention belongs to the technical field of microorganisms and particularly relates to a kitchen garbage bio-drying and stabilizing microbial agent as well as a preparation method and an application thereof. According to the technical scheme, pseudomonas aeruginosa is conserved in CGMCC (China General Microbiological Culture Collection Center) with the conservation number being CGMCC No.17868.By means of the pseudomonas aeruginosa, microbial composition including starch degrading bacteria, protein degrading bacteria, cellulose degrading bacteria, oil degrading bacteria and deodorization bacteria is provided; and the oil degrading bacteria comprise pseudomonas aeruginosa. The microbial composition can treat kitchen garbage in a targeted manner and achieve the purposes of dewatering, drying, reduction and recycling of the kitchen waste.

The invention relates to a biology preparation for reducing nitrosamine content in tobacco, and the manufacturing method and application, which belongs to biology technology field. The fungus Pseudomonas aeruginosa KenLXP30 product in the invention is preserved in normal microbe centre of china microbe preservation management committee in Jul 28 2003, the preserving number is CCTCC NO.0985. The invention picks out a fungus KenLXP30 in large amount of tobacco inner bacteria which can eliminates the amino nitrous acid content in tobacco, produces the biology preparation which can reduce the hard of tobacco. The preparation can reduce the amino nitrous acid content in white rib tobacco prominently, and it has good application value in tobacco industry.

The invention provides a compound bacterial agent for purifying a black and odorous water body and a preparation method thereof. The compound bacterial agent comprises the following components: actinomycetes, bacillus subtilis, nitrifying bacteria, lactic acid bacteria, candida utilis and pseudomonas aeruginosa in a weight ratio of 2:2:2:1:1:1. The invention has the following advantages: the COD content, ammonia nitrogen content and phosphate salt in the black and odorous water body can be effectively reduced, and the odor of the black and odorous water body can be eliminated; and meanwhile, by adding the ammonia nitrogen removal strain, the transparency of the water body can be gradually improved.

The invention relates to the technical field of medical detection, and in particular, relates to a primer and probe combination and a kit for detecting human pathogenic bacteria. The kit comprises theprimer and probe combination for detecting pseudomonas aeruginosa, escherichia coli, klebsiella pneumoniae, staphylococcus epidermidis, staphylococcus aureus, enterococcus faecium, human staphylococcus and acinetobacter baumannii. According to the kit, the primer and probe combination is adopted, multiple digital PCR is combined, the pathogenic bacteria types and the target nucleic acid copy number in the detection range can be rapidly and accurately identified, the detection method has high sensitivity, and positive detection of low-copy nucleic acid fragments of pathogenic bacteria is guaranteed; meanwhile, the variety of the pathogenic bacteria or the change of the copy number of the target nucleic acid fragment in the body of a pathogenic bacteria infected patient can be dynamically monitored, the treatment effect is assisted and evaluated in time, and reference is provided for optimization of a doctor clinical scheme.

The invention discloses a loop-mediated isothermal amplification (LAMP) detection primer pair of Pseudomonas aeruginosa, a detection method and a detection kit. Aiming at the ecfX gene of Pseudomonas aeruginosa, the invention designs and screens a set of specific detection primer pair, a detection kit containing the detection primer pair and an LAMP detection method utilizing the detection kit. The detection method is adopted to detect a sample to be detected, specially for drinking water to confirm whether the sample contains the special gene segment of Pseudomonas aeruginosa and further confirm whether the sample contains Pseudomonas aeruginosa. The detection kit and detection method disclosed by the invention have high sensitivity and specificity and short detection time, and the wholeprocess from sample processing to result reporting is only 24h; and the polymerase chain reaction (PCR) amplifier and electrophoresis meter are not adopted, the operation process is simple and the method and kit are especially suitable for the self-checking of grassroots detection institutions and drinking water processing enterprises.

The invention discloses a microbial limit detection method of miconazole nitrate suppository. The method comprises dissolving sample with sodium chloride-peptone buffer solution (pH 7.0) containing 10 vol% polysorbate 80 (Tween 80), adding 20ml of isopropyl myristate, and washing with aqueous solution containing 0.1 vol% polysorbate 80 and 0.1 wt% peptone. Membrane filtration method is adopted for bacteria, mildew, yeast, Staphyloccocus aureus and Monilia albican, with recovery rate all above 70%. Culture medium dilution method is adopted to detect Pseudomonas Aeruginosa. The addition of isopropyl myristate can accelerate fat/water layer separation, shorten experiment time, stabilize flow rate, reduce test cost, and achieve strong operability.

A genetic recombinant method of pseudomonas aeruginosa for high-yield producing rhamnolipid includes following steps: (1) designing a primer for amplifying RhlAB gene according to the RhlAB gene sequence of rhamnolipid synthetase; (2) with chromosome of wild pseudomonas aeruginosa producing rhamnolipid as a template, performing PCR amplification to the RhlAB gene and performing rubber cutting purification to target genes through a purification kit; (3) performing enzyme digestion to the purified RhlAB gene to introduce the purified RhlAB gene into an escherichia coli-pseudomonas aeruginosa shuttle expression vector pAK1900 to obtain new plasmid pAK1900-AB; (4) introducing the constructed shuttle expression vector pAK1900-AB into a recipient bacterium pseudomonas aeruginosa competent cell through a heat-shock conversion method, coating a carboxyl-benzyl resistance plate with the competent cell and performing PCR verification to the constructed engineering bacteria; and (5) performing fermentation in a shake flask and measuring the yield of the rhamnolipid in a sulfuric acid-anthranone manner. The method can greatly reduce the production cost of the rhamnolipid, provides basis for industrial application of the rhamnolipid and can promote high-yield production of the rhamnolipid.

The invention discloses a Pseudomonas aeruginosa strain PAH-1 capable of degrading PAHs, and its preservation number is CGMCC No.4773. Pseudomonas aeruginosa of the present invention can use humus as an electron acceptor under anaerobic conditions to oxidize and degrade polycyclic aromatic hydrocarbons, and can significantly improve the degradation rate of phenanthrene under glucose or fructose and phenanthrene co-metabolism conditions, and can be widely used in mild In situ bioremediation of polycyclic aromatic hydrocarbon contaminated soil.

The invention relates to a synergistic effect of multiple bacterial strains on degradation of polyphenol pollutants and a research method thereof. According to the invention, a bacterial suspension is prepared from one or a mixture of two or three selected from the group consisting of pseudomonas aeruginosa GIMT1.074, sphingobacterium multivorum CICC 23249 and ochrobactrum sp. BJS2; then the bacterial suspension is inoculated to inorganic a salt medium containing phenol, meta-cresol and 4-chlorophenol with different concentrations for culture; parts of a culture solution are taken out at different time intervals to measure concentrations of phenol, meta-cresol and 4-chlorophenol in the culture solution so as to determine degradation rates at different times, and cell concentrations in the culture solution are measured to investigate growth conditions of different cells. According to research on synergistic degradation effects of the three phenol degrading bacteria, a mixed bacterial strain has superior capability in degradation of mixed phenolic pollutants and has much better degradation efficiency and effects compared to every individual bacterial strain; moreover, biomass of the mixed bacterial strain is increased to two times of the biomass of an original optimal bacterial strain, i.e., the pseudomonas aeruginosa GIMT1.074.

Rhamnolipid is an anionic surfactant which is free of toxicity and pollution, biodegradable, high in selectivity and specificity and excellent in surface activity and has unique application prospects in multiple industrial fields such as medicine, food, cosmetics and environment protection. The invention provides Pseudomonas aeruginosa TIB-R02 CGMCC No.9376 with the high rhamnolipid yield and the high conversion rate through screening. Related fermentation equipment is transformed according to the fermentation characteristic of Pseudomonas aeruginosa, and a series connection fermentation experiment of 300L fermentation tanks is realized. According to the production state of the producing strain and the characteristic of the equipment, the production process of producing rhamnolipid in a fermented mode through Pseudomonas aeruginosa TIB-R02 CGMCC No.9376 is developed, and while the rhamnolipid yield is high, the liquid leakage problem caused by surface activity brought by a great deal of rhamnolipid can be controlled. By using the equipment and the fermentation process, when palm oil with the concentration of 75 g/L serves as a carbon source, the rhamnolipid yield reaches 60.6 g/l, and the conversion rate reaches 80% or more. Therefore, the strain and the production process have good industrial application prospects.

The invention discloses pseudomonas aeruginosa JT86 and an application of the pseudomonas aeruginosa JT86 in prevention and treatment of sclerotiniose. The pseudomonas aeruginosa JT86 is screened fromsoil, and the strain is collected in the Guangdong Microbiological Culture Collection Center on September 30, 2019, and has a collection number of GDMCC NO:60799. The strain and a volatile matter thereof can completely inhibit sclerotium germination of rhizoctonia solani, sclerotium rolfsii and sclerotinia sclerotiorum and inhibit growth of sclerotium hyphae, so that the sclerotium hyphae have flakes and wrinkles; the strain also has a good inhibition effect on the rhizoctonia solani, the sclerotium rolfsii, the sclerotinia sclerotiorum, magnaporthe grisea, fusarium oxysporum f. sp. cubense,brassica parachinensis anthracnose, colletotrichum gloeosporioides and colletotrichum capsici; and therefore, the strain can treat recalcitrant soil-borne pathogenic bacteria in soil in a targeted manner, effectively inhibits the sclerotiniose, and has a good application prospect in preparation of a soil treatment agent for preventing and treating soil-borne fungal diseases.

The invention discloses a method for producing a seedling raising substrate by using cotton straw and a substrate prepared with the same. The method comprises the following steps of: smashing cotton straw into sections of 10-50 millimeters; adding water till the water content is 60-80 percent according to mass percentage; adding 2-3kg of urea, 0.5-1kg of calcium superphosphate, 1-2kg of gypsum and 50-100g of fermentation inoculum into each cubic meter of substances obtained in the last step according to the volume ratio; mixing uniformly; and sealing, piling and fermenting at the air temperature of 15-40 DEG C, wherein the adopted compound fermentation inoculum is prepared by mixing pseudomonas aeruginosa, streptomyces albus and flavobacterium in the ratio of 2:3:1, and can be used by fermenting, airing in a scattered way and drying. In the method, a main raw material is derived from a large amount of waste cotton straw generated during the production of cotton, the raw material source is rich, a preparation process is simple, the aim of utilizing resources is fulfilled, and resource waste of cotton straw caused by direct smashing and returning to field can be reduced; and the method has a wide application value.

Traditional fermentation generally adopts a freshwater resource. The invention discloses a novel seawater fermentation strain generating a biosurfactant. Fermentation with natural seawater as fermentation water and waste edible grease as a single carbon source is carried out to produce the biosurfactant rhamnolipid. Seawater fermentation enterprises can be built in coastal regions lacking a freshwater resource and having bad water quality to solve the dependence of the traditional fermentation industry on the freshwater resource. The strain is separated and purified through sweater enrichment culture, hypersalemia flat primary screening, synthetic medium secondary screening and seawater shaking bottle fermentation verification, and is determined as Pseudomonas aeruginosa through physiologic biochemical experiments and molecular identification analysis, is preserved in China General Microbiological Culture Collection Center on April 25, 2014, and has a preservation number of CGMCC No.9091. The strain uses the advantages of the seawater fermentation, and the obtained crude product rhamnolipid can be used in regional market promotion, and has a potential application prospect in the fields of the agriculture, the petroleum chemical industry, environment treatment, soil restoration, the cosmetic industry, the pharmacy industry and the food industry.

The invention provides a group of peptide nucleic acid-cell penetrating peptide antisense antibacterial sequences taking bacterial gene rpoD as a target. The cell penetrating peptide-mediated antibacterial RNA polymerase sigma 70 factor gene rpoD which is antisense nucleic acid of a specificity target can selectively inhibit the expression of in-vivo ropD genes of Gram-negative bacterium (containing sensitive and multidrug resisting clinical pathogenicity Escherichia coli, salmonellatyphimurium, Klebsiella pneumoniae and pseudomonas aeruginosa) or gram-positive bacterium (containing sensitiveand multidrug resisting staphylococcus aureus) and further inhibit bacteria growth and reproduction, thus having the advantages of good antibacterial effect, low toxicity, good stability and better tolerance. The invention also discloses a chemical preparation method of a peptide nucleic acid-cell penetrating peptide solid phase. The antibacterial peptide synthesized in the invention can be used for preparing multidrug-resistant bacteria infection proofing antisense drugs and has the potency of being developed into a broad-spectrum antisense antibacterial agent which is expressed by efficiently transferred and peculiarly blocked bacterium disease-causing genes.

The invention relates to a microbial compound fungicide for treating oil base drilling cuttings as well as a preparation method and application of the microbial compound fungicide. The compound fungicide comprises pseudomonas aeruginosa with a collection number of CGMCC NO:8983 and acinetobacter baumannii with the collection number of CGMCCNO:8984. The invention further discloses the preparation method of the microbial compound fungicide and application of the compound fungicide in treatment of oil base drilling cuttings. The preparation method includes preparation methods of liquid and solid fungicides.