90results about How to "Antagonistic" patented technology

The invention discloses bacillus subtilis A16, which is preserved in China Center for Type Culture Collection (CCTCC) with CCTCC NO. 209105. The bacterial colony of the Bacillus subtilis A16 is oyster white on a beef extract peptone culture medium, has irregular shape and no luster, is not wet, has irregular edge, has diameter of 0.5-1cm, and has microfold surface, straight rod-shaped thallus and elliptic spore. The invention also discloses a preparation method and application of the bacillus subtilis A16. The bacillus subtilis A16 has broad bacteriostasis, can suppress exserohilum turcicum and helminthosporium maydis, has higher antagonism for exserohilum turcicum bacteria, helminthosporium maydis bacteria, maize curvularia leaf spot fungus, verticillium dahliae kleb, wheat scab pathogen, colletotrichum musae and banana vascular wilt bacteria, and has high prevention cure efficiency, and good safety and development application prospect.

The invention discloses bacillus velezensis HNt7 with broad-spectrum antibacterial activity as well as a preparation method and application. The antagonistic bacterium bacillus velezensis HNt7 has a number of CCTCC (China Center for Type Culture Collection) M 2018022. The preparation method comprises the following steps: A, selecting a bacillus velezensis HNt7 strain individual bacterial colony under a sterile condition and inoculating into a culture medium; adjusting the temperature and carrying out shake culture; B, filling a fermenting solution into each bottle and adding individual bacterial colony bacterium liquid according to the ratio; putting the bottle into a large-amplitude constant-temprature freezing shaking table and carrying out the shake culture; C, separating the antagonistic bacterium: immersing with alcohol with concentration and rinsing with sterile water; carrying out dark culture in a culture box and transferring a grown bacterial colony into a new culture medium and carrying out lineation separation and purification on the bacterial colony; D, after separating and purifying, obtaining the bacillus velezensis HNt7. The bacterium is used for preparing a medicinefor treating or preventing pear fruit rot disease. The strain has a remarkable disease-prevention effect and the prevention effect reaches 70.76 percent or more. After the bacillus velezensis HNt7 isapplied to pear trees, advanced defoliation of the pear trees is remarkably inhibited and the tree vigor is easy to enhance.

The invention discloses an environment-friendly fertilizer for strawberries and a preparation method thereof. The environment-friendly fertilizer is prepared from the following raw materials in parts by weight: 70-80 parts of organic fertilizer, 10-25 parts of inorganic fertilizer, 0.5-1 part of minor element, 0.1-0.3 part of fermentation composite bacteria, 1-2 parts of ferment bacteria, 3-7 parts of humic acid, 3-7 parts of amino acid and 0.2-0.7 part of antibiotics. The method comprises the following steps: uniformly mixing the fermentation composite bacteria with water to obtain a dilute bacterium solution; adding the dilute bacterium solution into the organic fertilizer, uniformly mixing, piling, and circularly overturning to obtain primary leavening; adding the ferment bacteria, fermenting until the materials are completely rotten, drying at low temperature, and pulverizing to obtain secondary leavening; and finally, adding the inorganic fertilizer, minor element, humic acid, amino acid and antibiotics, uniformly mixing, granulating, and sorting to obtain the environment-friendly fertilizer for strawberries. The environment-friendly fertilizer for strawberries is mainly prepared from agriculture and animal husbandry wastes, thereby solving the problem in crop nutrient supply and restricting the pathogenic microorganisms from reproduction.

The invention relates to a stachyose probiotics solid beverage which changes nutritional properties of food and comprises the following components by weight percent: 5-20 percent of stachyose, 2-10 percent of bifidobacterium powder, 2-10 percent of lactobacillus acidophilus powder, 3-8 percent of whey protein powder, 5-15 percent of milk powder, 10-40 percent of grain puffing powder, 5-15 percent of steviol glycoside, 5-15 percent of maltodextrin, 5-15 percent of citric acid and 0.5-2 percent of edible essence, wherein the sum of weight percentage of all the components is 100%. The stachyose probiotics solid beverage adopts natural raw materials, is scientific in formulation, nutritionally balanced, unique in flavor, and sweet and sour, and palatable in taste, not only is beneficial to intestinal health, but also has the health functions of improving immunity of the organisms, delaying senescence, inhibiting tumor and regulating blood lipids, and is convenient to carry and brew.

The invention relates to bacillus subtilis CH-001, which was collected in China Center for Type Culture Collection (CCTCC) on April 8th, 2011. The collection number is CCTCC NO: M 2011111. The bacillus subtilis has the characteristic of degrading keratin in a broad spectrum manner and has mild degradation and fermentation conditions, high enzyme production activity and high stability. The bacillus subtilis can generate high-activity keratinase, can degrade collagen, has antagonism on pathogenic bacteria such as staphylococcus aureus and the like, has the characteristics of probiotics, and is applicable to engineering bacteria development for fermentation production of animal protein feed additives and keratinase. The bacillus subtilis avoids the conflict between high-activity keratinase generated by most microorganisms and pathogenicity of the microorganisms on human or poultry and the broad spectrum characteristic on the keratin, and the antagonism on the related pathogenic bacteria is researched. The bacillus subtilis is a low-carbon, economic, high-efficiency, safe and green biological resource and has huge potential value for creation of economic and social benefits.

The invention discloses a Bacillus subtilis Y13 with the preservation number of CCTCC NO.M 208264, for effectively controlling oil tea anthrax, pertaining to the field of plant protection technology. At the same time, the invention discloses its separation method and applications, the Bacillus subtilis Y13 is obtained by flat plate facedown experiment screening, and can effectively inhibit the growth of oil tea anthrax bacteria, which has high proofing effect, good environment safety and favorable development and application prospects.

The invention discloses a soil conditioner capable of reducing activity of heavy metal in soil and a preparation method thereof. The soil conditioner is prepared from sulfydryl modified plant straw powder, an inorganic porous material, caustic dross, humic acid mineral powder, a compound microbial fertilizer and a heavy metal chelating agent. According to parts by weight of the sulfydryl modifiedplant straw powder, the inorganic porous material, the caustic dross, the humic acid mineral powder, the compound microbial fertilizer and the heavy metal chelating agent, the sulfydryl modified plantstraw powder is 3 to 7 parts, the inorganic porous material is 15 to 45 parts, the caustic dross is 1 to 5 parts, the humic acid mineral powder is 20 to 40 parts, the compound microbial fertilizer is2 to 8 parts, and the heavy metal chelating agent is 0.5 to 3 parts. The soil conditioner disclosed by the invention can effectively reduce activity of the heavy metal in acidic heavy metal contaminated soil and further has the advantages of low cost, easiness in implementation, greenness and environmental friendliness.

The invention discloses a ginger multi-component compounded microorganism fertilizer and a preparation method thereof. The compounded microorganism fertilizer for preventing ginger bacterial wilt comprises the following components in parts by weight: 3-6 parts of bacillus megaterium, 1-4 parts of bacillus laevolacticus, 5-8 parts of a compound microorganism bacterium agent, 900-1000 parts of an organic fertilizer, 440-480 parts of an inorganic fertilizer, 40-50 parts of a micronutrient fertilizer, 30-32 parts of an adhesive and 0.4-0.6 part of an effect-stabilizer auxiliaries. The utilization rate of the multi-component microorganism fertilizer is enhanced; the fertilizer has the effects of remarkable growth promotion, insect killing, sterilization and weeding and is pollution-free and low in residue, thereby having good economical and social benefits.

The invention discloses bacillus licheniformis 201 for high effectively preventing banana wilt, and the preservation number of the bacillus licheniformis 201 is CCTCCM207092. Bacterial colony on beef extract-peptone medium is greyish-white, round, flat and lackluster, the edge of the bacterial colony is in order, the diameter of the bacterial colony is generally 2 to 3 millimeters, bacteria are of straight rod shape, the size of the bacteria is 2.06* 0.70 micrometers, and spore is of elliptical shape. Simultaneously, the invention also discloses a process for preparation and usage. The bacillus licheniformis 201 is capable of strongly preventing fusarium oxysporum and has wide antimicrobial spectrum, which is capable of performing antagonistic action on various pathogenic fungi, such as fusarium oxysporum, fusarium oxysporum f. sp. niveum, fusarium oxysporum f. sp. lycopersici, gloeosporium musarum, colletotrichum gloeosporioides, maize curvularia leaf spot fungus, verticillium wilt pathogen, wheat scab pathogen and the like, furthermore, the invention is high effective in prevention, good in environmental security and good in the prospect of development and application.

The invention discloses a Bacillus licheniformis 201, which is preserved in China Center for Type Culture Collection that is located in Wuhan University, Logosun, Wuchang, Wuhan, Hubei, and with CCTCC NO. of M207092. The invention simultaneously discloses a preparation method and applications of the Bacillus licheniformis 201, which has strong bacteriostasis of banana wilt fusarium, wide bacteriostasis spectrum and antagonism to various pathogenic fungi including banana wilt fusarium, watermelon wilt fusarium, tomato wilt fusarium, banana colletotrichum, gloeocystidium colletotrichum, corn leaf spot curvularia, cotton verticillium lecanii, gibberella zeae, and the like, and the Bacillus licheniformis 201 has high control efficiency, good environmental security and good development and application prospect.

The invention provides a composite fertilizer for repairing lead-polluted soil, which includes nitrogen, phosphorus and potassium nutrients and the following components, in proper ratio: agricultural rare earth nitrates, waste catalyst, chitosan, humic acid mineral powder, sepiolite, plant ash, kaolin and zeolite. When the fertilizer is applied in lead-polluted soil, lead in soil is effectively passivated and immobilized under a synergistic effect of the components above, and meanwhile, the polluted soil is effectively repaired, so that activity of lead in soil is significantly reduced and adsorption of crops to lead is effectively reduced, thereby reducing lead content in crops significantly. The composite fertilizer is excellent in stability and environment-friendly property, is free of secondary pollution, is low in cost and is easy to popularize in large scale. When the fertilizer is applied in lead-polluted soil, the use quantity is only 10-200 kg / hm<2>. The composite fertilizer can significantly reduce the lead content in crops, so that the lead content in crops reaches national food safety standard.

Disclosed is a novel compound that acts as an antagonist for P2X3 and / or P2X2 / 3 receptors. Also disclosed is a pharmaceutical composition that exhibits analgesic activity or acts to ameliorate urinary problems, said composition containing: a compound represented by formula (I), a pharmacologically permitted salt thereof, or a solvate of either. In the formula, Rh and Rj together form a bond; Ra and Rb, and / or Rd and Re, together form an oxo or the like; Rc represents hydrogen, a substituted or unsubstituted alkyl, or the like; -Rf represents -(CR4aR4b) n -R2; R4a and R4b represent hydrogen, a substituted or unsubstituted alkyl, or the like; R2 represents a substituted or unsubstituted cycloalkyl or the like; n represents an integer from 1 to 4; -Rg represents -X-R3; -X- represents -O-, -S-, or the like; and R3 represents a substituted or unsubstituted cycloalkyl or the like.

The invention discloses a biocontrol streptomyces for preventing and treating camellia oleifera diseases and its application. A bacterial strain of the invention is streptomyces globisporus supsp.globisporus CSUFTAC0906002 and the preservation number is CCTCC M 2011012. The method comprises that: mixing fermentation broth of the bacterial strain with rice bran, naturally air-drying and grinding to produce raw powder; adding 40wt% of raw powder, 38wt% of kaolin, 10wt% of sodium lignosulfonate, 7wt% of SDS and 5 wt% of humic acid for preparing inocula; diluting inocula for directly applied to prevent and treat camellia oleifera diseases by a sprinkling and pouring root mode. The bacterial strain has preventive and therapeutic effect on camellia oleifera diseases, and has an antagonistic action on a plurality of pathogenic fungi. The bacterial strain has the advantages of various action modes and rapid breeding speed to the pathogen, especially has strong inhibiting effect on camellia anthracnose. The invention has important significance on preventing and treating camellia anthracnose, which has good application prospect for developing.

Disclosed is a novel compound having P2X3 or P2X2 / 3 receptor antagonistic effect. Specifically disclosed is a compound represented by formula (I). (In the formula, (a) Ra and Rb combine together to form =Z, and Rc is a group represented by R1c, or alternatively (b) Rb and Rc combine together to form a bond, and Ra is a group represented by -Y-R1a; R1a and R1c each independently represents a hydrogen atom, a substituted or unsubstituted alkyl group or the like; R2 and R3 each independently represents a substituted or unsubstituted aryl group or the like; R4a and R4b each independently represents a hydrogen atom, a substituted or unsubstituted alkyl group or the like; X represents -N(R5)- or the like; R5 represents a hydrogen atom, a substituted or unsubstituted lower alkyl group or the like; Y represents -O- or the like; Z represents =O or the like; and n represents an integer of 0-4.).

The invention relates to the field of micro-organisms, and particularly relates to a multifunctional Streptomyces amritsarensis and application thereof. Streptomyces amritsarensis HG-16, with a preservation number of CCTCC-M-2018237. The streptomycin cannot only kill the microcystin, but also antagonize the fusarium graminearum. The fermentation liquid and the active secretion can effectively inhibit the production of the microcystin in a short time, can inhibit the expression of the synthetic gene of 90% microcystin within 9 hours, and can completely inhibit the expression of the microcystingene after 36 hours. Three days after the addition of the Streptomyces, the inhibition rate of the algae toxin is 93%; while for the algicidal bacteria which has been reported to have the effect of inhibiting the production of the microcystin needs more than 3 days to inhibit the synthesis of the microcystin.

The invention discloses an oil-tea disease-resistant bacterial strain and an application thereof, in particular relates to an oil-tea anthracnose-resistant bacterial strain belonging to the technical field of plant protection. The bacterial strain is Bacillus brevis Z26, and the preservation number of the bacterial strain is China center for type culture collection (CCTCC) No: M20110113. The oil-tea disease-resistant bacterial strain is obtained by first screening by means of the a confrontation test and second screening by means of liquid fermentation liquid , can be effectively preventing and curing various plant diseases such as oil-tea anthracnose, oil-tea root rot, oil-tea half mad, citrus anthracnose, citrus root rot, aloe root rot and the like, particularly can be used for effectively restraining the growth of oil-tea anthracnose germ, is high in prevention efficiency, is good in environment security, and is very good in development and application foreground.

The invention relates to a cadmium-contaminated soil remedying agent, and belongs to the field of soil remediation. Attapulgite and isocyanate are mixed and modified by a silane coupling agent, so that a molecular bridge between an inorganic component interface and an organic component interface can be built to enhance the acting activity; meanwhile, Zn<2+> contained in salt can antagonize cadmiumions, so that absorption of the cadmium ions by plants and the toxic effect of the cadmium ions on crops are reduced, the mobility of the cadmium ions in soil is reduced, and the effects of passivating and remedying cadmium-contaminated soil are achieved; white mica and basalt are treated to form a three-dimensional crosslinked network so as to fix cadmium and reduce the bioavailability of the cadmium in the soil; protein can be destroyed by the cadmium ions, so that the viscosity is increased and a spatial structure becomes loose; the cadmium ions can be wrapped, so that the effect of reducing the mobility of the cadmium ions in the soil is achieved; and the problem that the current soil remedying agent commonly used for the cadmium-contaminated soil has poor remedying effect on the cadmium-contaminated soil is solved.

The invention belongs to the field of waste resource utilization, and particularly relates to an efficient chicken manure fermentation composite bacterium preparation. According to the invention, thepreparation is characterized in that the preparation is prepared from a yeast composition preparation with the total inoculum concentration being 5 percent and a lactobacillus composite preparation with the total inoculum concentration being 5 percent; the yeast composition preparation is prepared from Wickerhamomyces anomalus, candida tropicalis and candida utili according to the inoculum concentration ratio of 1:(2 to 3):(2 to 3), and is used for the aerobic fermentation of the chicken manure; the lactobacillus composite preparation is prepared from lactobacillus acidophilus, lactobacillus plantarum and lactobacillus farciminis according to the inoculum concentration ratio of 1:1:1, and is used for the anaerobic fermentation of the chicken manure. The chicken manure fermentation composite bacterium preparation provided by the invention comprises an aerobic fermentation preparation and an anaerobic fermentation preparation, so that the conventional whole-process anaerobic fermentationidea is broken; the real protein content in the chicken manure is effectively improved; the feeding value of the fermentation chicken manure is increased. The preparation can be directly used in practical production and has the important guidance significance on the subsequent further study.

The invention discloses a special disinsection and sterilization polyelement microorganism fertilizer for preventing cabbage downy mildew. The special disinsection and sterilization polyelement microorganism fertilizer comprises components of raw materials in parts by weight as follows: 3-5 parts of bacillus subtilis, 1-4 parts of trichoderma reesei, 4-8 parts of a fertilizer leavening agent, 1000-1100 parts of an organic fertilizer, 350-500 parts of an inorganic fertilizer, 65-85 parts of a trace element, 5-7 parts of a plant growth regulator, 25-35 parts of an adhesion agent, Chinese herbal medicinal ingredients and the like. The special disinsection and sterilization polyelement microorganism fertilizer for preventing the cabbage downy mildew can reduce the morbidity of the cabbage downy mildew greatly, and reduce bacterial and fungal diseases of leaves of a plant obviously, the plant can grow more vigorously, the yield of cabbages isincreased greatly, the quality is improved, and probiotics in the soil are also increased.

The invention discloses a pathogen verticillium antagonistic bacterium and application thereof, and belongs to the technical field of biological control over crop diseases. Bacillus pumilus Y106 is adopted as the pathogen verticillium antagonistic bacterium, the preservation number is CCTCC NO:M2014423, the preservation date is September 17, 2014, the preservation place is China Center for Type Culture Collection (CCTCC), and the preservation address is Wuhan University in Wuhan China. The bacterial strain is Gram-positive, mesophilic and aerobiotic and can resist the high temperature of 65 DEG C; the bacterial strain comprises the main morphological characteristics that the bacterial strain is shaped like a thin rod, the two ends are bluntly round, and the bacterial strain mostly exists in a single mode or in a chain-arranged mode. The pathogen verticillium antagonistic bacterium has the antagonistic effect on pathogen verticillium-verticillium dahliae, can inhibit germination of pathogen spores and can inhibit paroxysm of cotton verticillium wilt to a certain degree.

The invention discloses a micro-ecologic preparation for preparing a high-sterilization-capability micro-organism bacterial fertilizer and a preparation method thereof. The micro-ecologic preparation for preparing the high-sterilization-capability micro-organism bacterial fertilizer consists of the following compositions including a culture medium, a matrix and strains, wherein the strains include a strain A and a strain B. The preparation method of the micro-ecologic preparation for preparing the high-sterilization-capability micro-organism bacterial fertilizer comprises the three stages including a bacterium stage, a mould stage and a dehydration stage in the preparation process. The micro-ecologic preparation can increase soil fertility, prevent and control plant diseases and pests and is harmless and environment-friendly.

The invention provides 5-methyl-1,2,3-thiadiazole-containing 1,3,4-thiadiazole derivatives and their preparation method and use. The 5-methyl-1,2,3-thiadiazole-containing 1,3,4-thiadiazole derivatives are 1,2,3-thiadiazole-containing heterocyclic compounds. Intermediates of the 5-methyl-1,2,3-thiadiazole-containing 1,3,4-thiadiazole derivatives and the 5-methyl-1,2,3-thiadiazole-containing 1,3,4-thiadiazole derivatives respectively have a general structural formula III and a general structural formula IV. The invention discloses the general structural formula and the preparation method of the 5-methyl-1,2,3-thiadiazole-containing 1,3,4-thiadiazole derivatives, and also discloses a use of the 5-methyl-1,2,3-thiadiazole-containing 1,3,4-thiadiazole derivatives as bactericides, plant virus resistance agents and plant activators. After being mixing with agriculture acceptable assistants or synergists, the 5-methyl-1,2,3-thiadiazole-containing 1,3,4-thiadiazole derivatives can be used for preparation of bactericides, plant virus resistance agents and plant activators. The invention also discloses a use of the 5-methyl-1,2,3-thiadiazole-containing 1,3,4-thiadiazole derivatives combined with commercial insecticides, bactericides, plant virus resistance agents and plant activators in prevention and treatment on agricultural, forest and gardening diseases, insect pests and viral diseases, and also discloses a preparation method thereof.

The invention provides a set of production technology for preventing ralstonia solanacearum from growing and reproducing in solanaceae crops to increase crop yield by aiming at the defects of the prior art for controlling the damages of the ralstonia solanacearum to the crops. Brevibacillus choshinensis X23 has a wide bacteriostatic spectrum and good environmental safety and has antagonism effects to various pathogenic fungi, such as phytophthora parasitica var nicotianae, pyricularia grisea, alternaria alternate, phytophthora infestans and the like, so that the brevibacillus choshinensis X23 has favorable development prospect and application potential.

The invention discloses hydrogen-oxidizing bacteria pseudornonas fluorescens (SDW-16), which was deposited by the China Center for Type Culture Collection on September 19, 2018, and has an accession number of CCTCC No: M 2018640. The hydrogen-oxidizing bacteria have antagonism on cytospora sp. and botrytis cinerea and have a growth promotion effect on wheat, plant growth can be promoted, the yieldis improved, and the fertilizer efficiency and plant resistance are improved.

The invention discloses an endophytic antagonistic strain for controlling soft rot of camellia oleifera and application thereof. The strain is bacillus amyloliquefaciens sp. L46, with a collection number of CCTCC M2010324. The bacillus amyloliquefaciens L46 is obtained by flat-plate antagonistic test sieving, can be used for effectively suppressing growth of soft rot pathogenic bacteria of camellia oleifera, and has the advantages of high prevention effect, high environmental safety and broad development and application prospect.

The invention discloses walnut endophyte and a use thereof. The walnut endophyte is Bacillus amyloliquefaciens Ht-q6, is preserved in China general microbiological culture collection center in No.2, Courtyard 1, Beichen West Road, Chaoyang District, Beijing, Z. C. 100101 and has a preservation number of 9401. The walnut endophyte is separated and selected from walnut green peel, is Bacillus amyloliquefaciens and is named as Ht-q6. The walnut endophyte has antagonistic action on plant pathogenic fungi. The invention determines the optimal formula and fermentation conditions of the fermentation nutrient solution and provides a bacterial strain resource and a theoretical basis for development of an antibiological inoculants. The walnut endophyte is separated from plant tissue so that the endophyte easily colonize in plants and especially has stable effects of prevention and control of walnut diseases.

The invention relates to a streptomyces tubercidicus strain and an application thereof. The streptomyces tubercidicus strain 7-2 is preserved in China General Microbiological Culture Collection Center, has the preservation number of CGMCC No.14965 and is preserved on November 28, 2017. The streptomyces tubercidicus strain 7-2 has antagonistic effects on phomopsis asparagi, magnaporthe oryzae, mushroom monilinia fructicola, colletotrichum truncatum and other various plant pathogens. Fermentation broth has good control effect on asparagus stem blight, rice blast, rice false smut, colletotrichumdestructivum and mushroom brown rot, and significantly improves the control effect of bactericides when being mixed with the bactericides.

The invention discloses bacillus licheniformis 202 for high effectively preventing banana wilt, and the preservation number of the bacillus licheniformis 202 is CCTCCM207093. The invention simultaneously discloses a process for preparation and usage. The bacillus licheniformis 202 is obtained after being selected by being cultured face to face on a plate and doing pot experiment, which is capable of effectively preventing the growth of fusarium oxysporum and has wide antimicrobial spectrum. The invention is also capable of performing antagonistic action on various pathogenic fungi, such as cotton-wilt fusarium, verticillium wilt pathogen, fusarium oxysporum f. sp. niveum, fusarium oxysporum f. sp. lycopersici, curvulavia lunata or colletotrichum musae and the like, which is good in environmental security and good in prospect of development and application.

The invention discloses a cottonseed meal feed and a preparation method thereof. According to the cottonseed meal feed, the content of gossypol is less than or equal to 100mg / kg. The preparation method comprises the following steps: adding water 2-6 times of the weight of cottonseed meal to the cotton seed meal, uniformly stirring and heating for 1-3 hours; respectively culturing an aspergillus niger strain and a bacillus natto strain; adjusting the pH of the cottonseed meal subjected to pretreatment to 6.5-7.5, adding cane molasses, carrying out heat treatment at 60-90 DEG C for 1 hour, then inoculating the strains, and fermenting for 48-96 hours; and drying and crushing the fermented product to obtain detoxified cottonseed meal. The content of gossypol in the cottonseed meal obtained by final fermentation is less than or equal to 80mg / kg, the small molecule peptide and crude protein content is greatly increased and the cottonseed meal feed can completely used as an animal feed.